阅读说明：本技术 一种水稻种子处理剂及其应用 (Rice seed treating agent and application thereof ) 是由 胡立勇 吉晓晨 江洋 罗冉 袁国保 于 2021-08-26 设计创作，主要内容包括：本发明公开了一种水稻种子处理剂,由2-甲氧基-4-甲基苯酚和硫酸锌组成；还公开了其用于种子引发：将种子经次氯酸钠消毒冲洗后,采用浓度为45～90 mg/ml的2-甲氧基-4-甲基苯酚和0.16 mg/ml的硫酸锌溶液浸泡水稻种子,种子与处理剂按照1:5的比例在温度为26±1℃的黑暗环境中进行浸种引发12h；取出种子用清水冲洗3～5次后晾干；还公开了其用于种子包膜：采用浓度为90～180 mg/ml的2-甲氧基-4-甲基苯酚和1.6 mg/ml的硫酸锌溶液与包膜剂按2：8的比例混合均匀,然后加入4～5倍体积的水稀释形成包膜液,置于干净的器皿中；按照液：种=55：1的比例对种子进行包膜作业。本发明首次提出2-甲氧基-4-甲基苯酚在适宜浓度下进行种子引发或包膜,具有促进植物种子萌发、生根与茎叶生长的作用。(The invention discloses a rice seed treating agent, which consists of 2-methoxy-4-methylphenol and zinc sulfate; it is also disclosed for seed priming: after the seeds are disinfected and washed by sodium hypochlorite, soaking the rice seeds in a 2-methoxy-4-methylphenol solution with the concentration of 45-90 mg/ml and a zinc sulfate solution with the concentration of 0.16 mg/ml, and soaking the seeds and a treating agent in a dark environment with the temperature of 26 +/-1 ℃ according to the ratio of 1:5 for initiation for 12 hours; taking out the seeds, washing the seeds for 3-5 times by using clear water, and then airing the seeds; it is also disclosed for seed coating: adopting 90-180 mg/ml 2-methoxy-4-methylphenol and 1.6 mg/ml zinc sulfate solution and a coating agent according to the ratio of 2: 8, then adding water with the volume of 4-5 times of that of the mixture to dilute the mixture to form a coating liquid, and placing the coating liquid in a clean vessel; according to the liquid: seed = 55: 1, coating the seeds. The invention firstly proposes that the 2-methoxy-4-methylphenol is subjected to seed initiation or envelope at a proper concentration, and has the effects of promoting the germination, rooting and the growth of stems and leaves of plants.)

1. A rice seed treatment agent characterized by: it is a solution prepared by using 2-methoxy-4-methylphenol as a main material and zinc sulfate as an auxiliary material.

2. The rice seed treatment of claim 1, comprising the steps of:

adopting a sodium hypochlorite solution with the concentration of 0.1% according to the seed: sodium hypochlorite solution = 1:5 (m/v) disinfecting the seeds for 10min, and then washing the seeds with distilled water for 4-5 times;

soaking rice seeds in a 2-methoxy-4-methylphenol solution with the concentration of 45-90 mg/ml and a zinc sulfate solution with the concentration of 0.16 mg/ml, and soaking the seeds and a treating agent in a dark environment with the temperature of 26 +/-1 ℃ according to the ratio of 1:5 (m/v) for 12 hours; and taking out the seeds, washing the seeds for 3-5 times by using clear water, and then airing the seeds.

3. The rice seed treatment agent for seed coating according to claim 1, comprising the steps of:

adopting 90-180 mg/ml 2-methoxy-4-methylphenol and 1.6 mg/ml zinc sulfate solution and a coating agent according to the ratio of 2: 8 (v/v), adding water with the volume of 4-5 times of that of the mixture to dilute the mixture to form a coating liquid, and placing the coating liquid in a clean vessel; according to the liquid: seed = 55: 1 (ml/kg), using a special seed coating machine to atomize the coating solution and uniformly coat the coating solution on the surface of the seeds, and then rapidly drying the water in the coating solution on the surface of the seeds through a fluidized bed to ensure that the dried 2-methoxy-4-methylphenol is firmly attached to the surface of the seeds in a film form.

4. The application of the rice seed treating agent as claimed in claim 3, wherein the coating agent is Bian Liang jin Yi rice seed coating agent.

5. The application of the rice seed treating agent according to claim 3, wherein the special seed coating machine is a modern agricultural package 5BW50-100 type coating machine in Beijing.

6. The use of the rice seed treatment agent according to claim 3, wherein the fluidized bed is a reciprocating fluidized bed dryer model WLG-6X1.2 of Jiangsu Bo Si Wei chemical engineering Co.

Technical Field

The invention belongs to the field of plant growth conditioners, and particularly relates to a rice seed treating agent based on 2-methoxy-4-methylphenol, and an initiating and coating agent for rice seeds.

Background

With the increase of labor cost and the enhancement of the demand of farmers for simplifying high-efficiency cultivation, the area of applying the rice direct seeding technology in China is rapidly expanded in recent years. The direct seeding technology of rice is a cultivation technology for directly seeding dry rice or initiated (seed soaking and germination) bud and grain to a field in a manual, mechanical or unmanned aerial vehicle mode and the like without traditional seedling raising and transplanting. It can be divided into wet direct seeding, water direct seeding, dry direct seeding and the like. Compared with the traditional transplanting technology, the direct seeding technology does not need preparation of seedling beds, seedling management, preparation of local fields such as field soaking, field weeding and the like before transplanting, greatly reduces the consumption of fresh water resources, relieves the pressure of water resource shortage, reduces the investment of rural labor force and reduces the production cost of rice. Therefore, the direct seeding technology has the advantages of labor saving, water saving, cost saving and the like. However, the low emergence rate, irregular emergence and low growth vigor of direct sowing in the field are critical problems affecting the direct seeding and seedling formation of rice. How to improve the seedling forming capability of plants by improving the seed activity has become an important technology for direct seeding and planting of rice at present.

The existing research shows that the plant growth regulator can effectively control the growth and development of plants and improve the yield of rice. The chemical control technology of rice by taking the application of plant growth regulator as a means has become an important technology for high-yield, high-quality and high-efficiency production of rice and plays an increasingly important role.

The plant growth regulating substance is applied to seed treatment before sowing, the environmental conditions, concentration and time for using the medicament are relatively easy to control, the application effect is stable compared with that of a field, the influence of the regulating substance can be continued to the growth and development of the plant at the later stage, and the plant morphological index, physiological and biochemical reaction and stress resistance are continuously influenced, so that the use effect of the regulator is improved, and meanwhile, the labor investment for applying the medicament in the plant growth process and the possibly generated adverse environmental influence can be remarkably reduced. Therefore, the seed treatment is a technology with high efficiency, environmental protection and wide application prospect.

2-Methoxy-4-methylphenol (2-Methoxy-4-Methyl Phenol), also known as 4-Methyl guaiacol, is an organic chemical substance naturally present in anise seed essential oil, jasmine essential oil, ylang-ylang essential oil, coffee, cocoa, beer, rum, sherry, malt liquor, whisky. Has the sensory characteristics of spicy fragrance, medicinal fragrance, clove and vanilla fragrance. Can be used as flavoring agent for whisky, or blending edible essence such as flos Caryophylli, smoked meat, vanilla, etc. and tobacco essence. However, at present, no research and report on the adoption of 2-methoxy-4-methylphenol as a plant growth regulator and a seed treatment agent exist. The development of the 2-methoxy-4-methylphenol is beneficial to high-efficiency crop production and green ecological environment protection.

Disclosure of Invention

One of the purposes of the invention is to provide a rice seed treating agent, and relates to the application of 2-methoxy-4-methylphenol in regulating the growth activity of plants.

The technical scheme adopted by the invention for solving the technical problems is as follows: a rice seed treating agent is a solution prepared by taking 2-methoxy-4-methylphenol as a main material and zinc sulfate as an auxiliary material. The 2-methoxy-4-methylphenol (Gamma-butyrolactone) is a colorless oily liquid organic matter, and the molecular formula is C₈H₁₀O₂Molecular weight 138.16, structural formula:

。

the invention also aims to provide the seed treatment agent for seed priming, which comprises the following steps:

adopting a sodium hypochlorite solution with the concentration of 0.1% according to the seed: sodium hypochlorite solution = 1:5 (m/v) disinfecting the seeds for 10min, and then washing the seeds with distilled water for 4-5 times; soaking rice seeds in a 2-methoxy-4-methylphenol solution with the concentration of 45-90 mg/ml and a zinc sulfate solution with the concentration of 0.16 mg/ml, and soaking the seeds and a treating agent in a dark environment with the temperature of 26 +/-1 ℃ according to the ratio of 1:5 (m/v) for 12 hours; and taking out the seeds, washing the seeds for 3-5 times by using clear water, and then airing the seeds.

The invention also aims to provide the seed treating agent for seed coating, which comprises the following steps:

adopting 90-180 mg/ml 2-methoxy-4-methylphenol and 1.6 mg/ml zinc sulfate solution and a coating agent according to the ratio of 2: 8 (v/v), adding water with the volume of 4-5 times of that of the mixture to dilute the mixture to form a coating liquid, and placing the coating liquid in a clean vessel; when the crop seeds are subjected to coating operation, the coating liquid comprises the following components: seed = 55: 1 (ml/kg), using a special seed coating machine to atomize the coating solution and uniformly coat the coating solution on the surface of the seeds, and then rapidly drying the water in the coating solution on the surface of the seeds through a fluidized bed to ensure that the dried 2-methoxy-4-methylphenol is firmly attached to the surface of the seeds in a film form.

The rice seed treating agent is a Bian Liang Jinyi rice seed coating agent.

The application of the rice seed treating agent is characterized in that a special seed coating machine is a Beijing modern agricultural 5BW50-100 type coating machine.

The application of the rice seed treating agent can be realized by drying the rice seed by a fluidized bed after the rice seed is coated by a seed coating machine. The fluidized bed is a WLG-6X1.2 reciprocating fluidized bed dryer of Jiangsu Bo Si Wei chemical engineering Co

The invention has the beneficial effects that: the 2-methoxy-4-methylphenol has low-concentration promoting and high-concentration inhibiting effects on plant seeds, can be used as a novel plant seed initiator component, is applied to rice or other crop seeds, can adjust the seed vitality, effectively accelerates the germination and emergence of the seeds, improves the emergence rate, and promotes the growth of seedlings.

Detailed Description

The present invention will be described in further detail with reference to examples, but the present invention is not limited thereto.

The invention relates to a rice seed treating agent, which is a solution prepared by taking 2-methoxy-4-methylphenol as a main material and zinc sulfate as an auxiliary material.

The invention also relates to application of the 2-methoxy-4-methylphenol in promoting seed germination and emergence. The 2-methoxy-4-methylphenol has low-concentration promotion and high-concentration inhibition effects on rice seeds, and can be used as a novel seed initiator component in the fields of agriculture, forestry, gardening and the like.

The embodiment provides an application method of an active ingredient seed treatment agent for promoting rice germination and emergence, which comprises the following steps:

seed priming: adopting a sodium hypochlorite solution with the concentration of 0.1% according to the seed: sodium hypochlorite solution = 1:5 (m/v) disinfecting the seeds for 10min, and then washing the seeds with distilled water for 4-5 times; soaking rice seeds in a 2-methoxy-4-methylphenol solution with the concentration of 45-90 mg/ml and a zinc sulfate solution with the concentration of 0.16 mg/ml, and soaking the seeds and a treating agent in a dark environment with the temperature of 26 +/-1 ℃ according to the ratio of 1:5 (m/v) for 12 hours; and taking out the seeds, washing the seeds for 3-5 times by using clear water, and then airing the seeds.

Coating the seeds: adopting 90-180 mg/ml 2-methoxy-4-methylphenol and 1.6 mg/ml zinc sulfate solution and a coating agent according to the ratio of 2: 8 (v/v), adding water with the volume of 4-5 times of that of the mixture to dilute the mixture to form a coating solution, and placing the diluted coating solution in a clean container. Coating agent (ml) and seed medicine: the seed ratio is 55: 1 (ml/kg). If a common container is adopted, the seeds are quickly stirred by a glass rod after being added, so that the coating solution is uniformly coated on the surfaces of the seeds, and then the seeds are dried in the shade and ventilated places for storage or sowing. If a coating machine is adopted to carry out coating operation on seeds, the coating solution is atomized in the coating machine and then uniformly coated on the surfaces of the seeds, and the coated seeds are quickly dried by a fluidized bed and then preserved or sowed.

The 2-methoxy-4-methylphenol is a colorless to light yellow liquid, is slightly soluble in water, is dissolved in organic flux such as ethanol and the like, and has a molecular formula of C₈H₁₀O₂Molecular weight 138.16, structural formula:

。

(1) test materials: the test variety is rice variety Huanghuazhan.

(2) The test treatment method comprises the following steps:

seed priming: according to the seed: treatment agent = 1:5 (m/v), disinfecting the mixture for about 10min by using a 0.1% sodium hypochlorite solution, then washing the mixture for 4-5 times by using distilled water, and washing off the residual solution of the sodium hypochlorite on the surface. The tested seeds are soaked in the 2-methoxy-4-methylphenol with the concentration of 60 mg/ml and the zinc sulfate solution with the concentration of 0.16 mg/ml for 12 hours, washed by clear water, dried and sown in a germination box.

Coating the seeds: the test seeds adopt 120 mg/ml 2-methoxy-4-methylphenol and 1.6 mg/ml zinc sulfate treating agent and coating agent according to the proportion of 2: 8 volume ratio (v/v) to uniformly coat the surface of the seeds with the coating solution, and airing or drying the coated seeds and then sowing the seeds in a germination box.

The treated seeds are placed in an artificial climate incubator for growth. The incubator is set to light (13000 lx)/dark (0 lx), and the light/dark time is set to 12 h/12 h. The night/day temperature was set at 25/30 ℃. The seeds were evenly placed in germination boxes (volume 12X 12 cm) covered with 3 layers of autoclaved filter paper and added with 12ml of distilled water, 50 seeds were placed in order in each germination box, and the procedure was repeated three times.

(3) The measuring and calculating method comprises the following steps: recording the germination rate every day after sowing; the rate of emergence was counted daily from the date of emergence. On day 7, sampling seedlings, and determining the fresh weight and the dry weight of the seedlings. And calculating indexes of germination rate, emergence rate, average germination time (MGT), Germination Index (GI) and the like of different crops.

And (4) after sowing, placing the incubator for 24h, and counting indexes such as germination rate, emergence rate and the like according to the same time every day. The germination rate of the seeds is counted by taking the 'radicle breaking through seed coat' as a standard, and the emergence rate is counted by taking the 'radicle exceeding the length of the seeds and the embryo reaching half of the length of the seeds' as a standard.

The indexes of germination rate, emergence rate, average germination time (MGT), average emergence time (MET), Germination Index (GI), Seedling Vigor Index (SVI) and the like are calculated according to the following formulas.

Germination rate: GP = Gn/100 × 100% (Gn represents the total number of germinated seeds);

emergence rate: EP = En/100 × 100% (En represents the total number of seeds that emerge);

average germination time:(gi represents the number of seeds germinated on day i, ti represents day i since sowing);

average time of emergence:(ei represents the number of seeds emerging on day i, ti represents day i since emergence);

germination index:；

seedling vitality index: SVI = GI x m. (m is individual seedling dry weight).

The results of the effect of the obtained 2-methoxy-4-methylphenol priming treatment on the emergence of seeds are shown in the following table.

The results of the effect of seed priming and coating treatments on germination and emergence obtained in this example are shown in the following table:

the results of the effect of the obtained seed priming and coating treatments on seedling growth are shown in the following table:

the results of the two tables show that after the appropriate concentration of the 2-methoxy-4-methylphenol determined by the invention is adopted for seed initiation and coating treatment, the indexes of the average germination and emergence time, the germination and emergence rate, the height and the root length of the seedling, the weight of the stem and the leaf of the seedling, the dry weight and the total dry weight of the root and the like of the drought-resistant rice can reach significant levels compared with the control. Has the effects of improving the seed vitality, effectively accelerating the seed germination speed, improving the germination rate and the emergence rate and promoting strong seedlings.

The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.