阅读说明：本技术 一种防治番木瓜害螨的增效组合物 (Synergistic composition for preventing and treating papaya mites ) 是由 陈燕 郑剑 陈仕淼 余江敏 潘祖建 何江 欧景莉 朱杨帆 黄雪梅 于 2021-08-30 设计创作，主要内容包括：本发明涉及农药技术领域,具体涉及一种防治番木瓜害螨的增效组合物。一种防治番木瓜害螨的增效组合物,所述增效组合物的有效成分由百里香酚与四螨嗪、伊维菌素或多拉菌素二元复配而成；其中,所述四螨嗪和百里香酚的质量比为1-5：10-1；所述伊维菌素和百里香酚的质量比为1-10：30-1；所述多拉菌素和百里香酚的质量比为1-20：40-1。本发明的增效组合物中两种有效成分以一定质量比进行复配时,共毒系数均大于120,表现出增效作用,这样可以显著提高对害螨的防治效果,降低农药施用量,同时还可以降低害螨的防治成本和延缓害螨抗药性的产生；在此基础上,添加农药学上可接受的农药辅料,可以制作成化学药剂,对开发防除害螨的新型化学药剂具有重要意义。(The invention relates to the technical field of pesticides, and particularly relates to a synergistic composition for preventing and treating papaya mites. A synergistic composition for preventing and treating papaya mites comprises the effective components of thymol and clofentezine, ivermectin or doramectin which are compounded in a binary manner; wherein the mass ratio of the clofentezine to the thymol is 1-5: 10-1; the mass ratio of the ivermectin to the thymol is 1-10: 30-1; the mass ratio of doramectin to thymol is 1-20: 40-1. When two effective components in the synergistic composition are compounded according to a certain mass ratio, the co-toxicity coefficient is more than 120, and the synergistic effect is shown, so that the control effect on pest mites can be obviously improved, the application amount of pesticides is reduced, the control cost of the pest mites can be reduced, and the generation of drug resistance of the pest mites can be delayed; on the basis, pesticide auxiliary materials acceptable in the pesticide pharmacy are added, so that the pesticide can be prepared into chemical agents, and the pesticide has important significance for developing novel chemical agents for preventing and killing harmful mites.)

1. The synergistic composition for preventing and treating the papaya mites is characterized in that the effective components of the synergistic composition are prepared by compounding thymol and clofentezine, ivermectin or doramectin; wherein the content of the first and second substances,

the mass ratio of the clofentezine to the thymol is 1-5: 10-1;

the mass ratio of the ivermectin to the thymol is 1-10: 30-1;

the mass ratio of doramectin to thymol is 1-20: 40-1.

2. The synergistic composition for controlling papaya mites as claimed in claim 1, wherein the mass ratio of clofentezine to thymol is 1: 5.

3. the synergistic composition for controlling papaya mites as claimed in claim 1, wherein the mass ratio of ivermectin to thymol is 1: 2.

4. the synergistic composition for controlling papaya mites as claimed in claim 1, where in the mass ratio of doramectin to thymol is 5: 1.

Technical Field

The invention relates to the technical field of pesticides, and particularly relates to a synergistic composition for preventing and treating papaya mites.

Background

Papaya is a evergreen soft woody small tree of papaya genus of family Caricaceae, native to southern Mexico and adjacent central America, and widely planted in Taiwan, Guangdong, Guangxi and Yunnan provinces of China. Ripe fruit of papaya can be used as fruit, immature fruit can be used as cooked vegetable or pickled vegetable, and can also be processed into products such as preserved fruit, fruit juice and jam, and its seed can be used for extracting oil, and its fruit and leaf can be used for medicinal purpose.

Like crops, in the planting process of papaya, the papaya is often attacked by plant diseases and insect pests, common pests include anthracnose, ringspot mosaic disease, downy mildew, pest mites, aphids, coccid, prodenia litura and the like, wherein the pest mites are one of main pest pests which harm papaya and mainly move on the back of papaya leaves to absorb juice, the damaged leaves lack green and turn yellow to form yellow spots, the yellow spots are connected into one piece or patch to influence the photosynthesis of plants, and the leaves fall off in severe cases to further influence the yield of the growth of papaya plants. Chemical agents are common means for preventing and controlling harmful mites, but a series of problems such as drug resistance, pesticide residue, environmental pollution and the like can be caused by long-term use of a single chemical agent, so that the development of novel pesticides for preventing and controlling harmful mites has important significance.

Research shows that many plant-derived phenolic substances show higher contact killing effect on pest mites, such as: eugenol, carvacrol, thymol, and the like. Application number CN200910088809.3 discloses a thymol mite-killing microemulsion and a preparation method thereof, which can effectively prevent and control multiple pesticide mites such as tetranychus cinnabarinus, tetranychus dermatioides and tetranychus bihami; meanwhile, the structural formula, the chemical name and the physical and chemical properties of thymol are also specifically disclosed in the patent.

Compounding the effective components of the pesticide with different action mechanisms is an effective and rapid way for developing new products of the pesticide and preventing and treating resistant plant diseases and insect pests at present. After different pesticide active ingredients are compounded, three action types can be shown: additive action, synergistic action and antagonistic action. Generally, the action types of different pesticide active ingredients after being compounded can be known only through a large number of tests. Screening the compound pesticide with synergistic effect can obviously improve the control effect, reduce the pesticide use amount, reduce the control cost, delay the generation of pest resistance and reduce the pollution to the environment, and is one of the important means of the current comprehensive control of plant diseases and insect pests. The inventor discovers that the synergistic effect is shown when thymol and clofentezine, ivermectin or doramectin are compounded in a certain mass ratio range through a large number of indoor biological activity tests, which has important significance for developing novel pesticides, and related compounding reports are not seen at present.

Disclosure of Invention

The invention aims to provide a synergistic composition for preventing and treating papaya mites, which solves the problem of long-term use of a single chemical agent.

In order to achieve the purpose, the invention provides the following technical scheme:

a synergistic composition for preventing and treating papaya mites comprises the effective components of thymol and clofentezine, ivermectin or doramectin which are compounded in a binary manner; wherein the mass ratio of the clofentezine to the thymol is 1-5: 10-1; the mass ratio of the ivermectin to the thymol is 1-10: 30-1; the mass ratio of doramectin to thymol is 1-20: 40-1.

Preferably, the mass ratio of the clofentezine to the thymol is 1: 5.

preferably, the mass ratio of the ivermectin to the thymol is 1: 2.

preferably, the mass ratio of the doramectin to the thymol is 5: 1.

compared with the prior art, the invention has the following beneficial effects:

when two effective components in the synergistic composition are compounded according to a certain mass ratio, the co-toxicity coefficient is more than 120, and the synergistic effect is shown, so that the control effect on pest mites can be obviously improved, the application amount of pesticides is reduced, the control cost of the pest mites can be reduced, and the generation of drug resistance of the pest mites can be delayed; on the basis, pesticide auxiliary materials acceptable in the pesticide pharmacy are added, so that the pesticide can be prepared into chemical agents, and the pesticide has important significance for developing novel chemical agents for preventing and killing harmful mites.

Detailed Description

In the following, the technical solutions of the present invention will be described clearly and completely, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples: indoor activity test after thymol compounding

Reagent to be tested: 99% of thymol technical, 95% of clofentezine technical, 99% of ivermectin technical and 98% of doramectin technical;

for testing harmful mites: tetranychus urticae adopted from papaya plants is bred, bred and hatched in a laboratory to obtain a first generation nymph;

test method (refer to NT/T1154.13-2008 Standard for indoor pesticide determination 13: leaf dish spraying method)

1. Preparing the original drug into single-dose mother liquor, setting a plurality of groups of mixture ratios, and preparing 5 gradient mass concentrations by using 0.1% Tween-80 solution to mix the single-dose mother liquor and each group of mixture ratios according to an equal ratio method for later use;

2. selecting papaya leaves with consistent growth, and making into a leaf disc by using a puncher; placing a wet sponge block in a culture dish, placing filter paper on the wet sponge block, placing leaf disks on the filter paper, and placing 2 leaf disks in each dish; inoculating test mites on the leaf discs, wherein each leaf disc is inoculated with 20 heads;

3. the spray pressure of the Potter spray tower was adjusted to 1.47X 10⁵Pa; placing the culture dish on a bottom plate of a Potter spray tower for spraying, wherein the spraying liquid amount is 1mL, each treatment is repeated for 4 times, and the treatment without the medicament is used as a blank control; taking out the liquid medicine after the liquid medicine is sunk for 1min and transferring to the temperature (25 +/-1) DEG C, the light cycle L: d, raising under the condition of (16: 8) h;

4.48h later, the death condition of the tested harmful mites is checked, the total number of the harmful mites and the number of the dead mites are respectively recorded, the corrected death rate of each treatment is calculated, the DPS software is used for processing and analyzing the test data, and the LC of each medicament is calculated₅₀And calculating the co-toxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.

P ═ K/N × 100, where P is mortality (%), K is dead number of worms, and N is total number of treated worms;

P₁＝(P_t－P₀)/(1－P₀) In which P is₁To correct for mortality (%), P_tTo address mortality (%), P₀Blank control mortality (%);

measured toxicity index (ATI) ═ standard medicament LC₅₀Test agent LC₅₀)×100；

Theoretical virulence index (TTI) ═ a agent virulence index × percentage of a in the mixture + B agent virulence index × percentage of B in the mixture;

co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ];

criteria are divided according to joint action: the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80< co-toxicity coefficient (CTC) <120 showed additive effect, and the experimental results are shown in tables 1-3.

TABLE 1 indoor bioactivity assay of Tetranyperazine and thymol combinations on Tetranychus urticae

Name and proportion of the medicament	LC50(mg/L)	ATI	TTI	CTC
					Clofentezine	19.32	100.00	--	--
Thymol	124.15	15.56	--	--
					Clofentezine 1: thymol 10	24.83	77.81	23.24	334.84
Clofentezine 1: thymol 8	10.37	186.31	24.94	746.90
					Clofentezine 1: thymol 5	7.23	267.22	29.63	901.71
Clofentezine 1: thymol 2	22.24	86.87	43.71	198.75
					Clofentezine 1: thymol 1	18.06	106.98	57.78	185.14
Clofentezine 2: thymol 1	8.22	235.04	71.85	327.10
					Clofentezine 3: thymol 1	16.74	115.41	78.89	146.29
Clofentezine 5: thymol 1	17.31	111.61	85.93	129.89

As can be seen from Table 1, the weight ratio of clofentezine to thymol is 1-5: the cotoxicity coefficients within the range of 10-1 are all larger than 120, which shows that the biological activity of the clofentezine and the thymol to the Tetranychus urticae after the compounding can show a synergistic effect, and particularly, the mass ratio of the clofentezine to the thymol is 1: and 5, the co-toxicity coefficient reaches 901.71, and the synergistic effect is particularly obvious.

TABLE 2 indoor bioactivity assay of Ivermectin and thymol combinations on Tetranychus urticae

Name and proportion of the medicament	LC50(mg/L)	ATI	TTI	CTC
					Ivermectin	2.63	100.00	--	--
Thymol	124.15	2.12	--	--
					Ivermectin 1: thymol 30	33.37	7.88	5.28	149.38
Ivermectin 1: thymol 20	15.03	17.50	6.78	258.11
					Ivermectin 1: thymol 10	13.35	19.70	11.02	178.82
Ivermectin 1: thymol 8	6.39	41.16	12.99	316.74
					Ivermectin 1: thymol 5	4.26	61.74	18.43	334.95
Ivermectin 1: thymol 2	1.14	230.70	34.75	663.97
					Ivermectin 1: thymol 1	2.46	106.91	51.06	209.39
Ivermectin 2: thymol 1	2.72	96.69	67.37	143.52
					Ivermectin 5: thymol 1	0.86	305.81	83.69	365.43
Ivermectin 8: thymol 1	1.92	136.98	89.12	153.69
					Ivermectin 10: thymol 1	2.31	113.85	91.10	124.97

As can be seen from table 2, ivermectin and thymol are present in a mass ratio of 1-10: the cotoxicity coefficients within the range of 30-1 are all larger than 120, which shows that the biological activity of the compounded ivermectin and thymol on the tetranychus urticae can be shown as a synergistic effect; particularly, the mass ratio of 1: 2, the co-toxicity coefficient reaches 663.97, and the synergistic effect is particularly obvious.

TABLE 3 indoor bioactivity assay of thymol and doramectin in combination with Tetranychus urticae

As can be seen from table 3, doramectin and thymol were added at a mass ratio of 1-20: the cotoxicity coefficients within the range of 40-1 are all larger than 120, which shows that the biological activity of the compounded doramectin and thymol on the tetranychus urticae can be shown as a synergistic effect; especially, the mass ratio of 5: when 1, the co-toxicity coefficient reaches 842.31, and the synergistic effect is particularly obvious.

In conclusion, when the thymol and the clofentezine, the ivermectin or the doramectin are compounded according to a certain mass ratio, the synergistic effect is shown on the biological activity of the papaya pest mites, and compared with a single component, the control effect can be effectively improved.