阅读说明：本技术 一种化妆品用刺五加提取物及其应用 (Acanthopanax senticosus extract for cosmetics and application thereof ) 是由 刘玉亮 苏荣生 邓静 吴晓璇 于 2021-08-10 设计创作，主要内容包括：本发明涉及IPC A61K36/25领域,尤其涉及一种化妆品用刺五加提取物及其应用。其制备步骤包括：S1.收集刺五加植物体,并对刺五加植物体进行干燥,粉碎；S2.将粉碎后的刺五加植物体加入第一溶剂中进行提取,得到提取液；S3.对提取液进行减压浓缩,加入第二溶剂,得到混悬液；S4.使用第三溶剂对悬混液进行萃取,得到刺五加提取物成品。本发明提供了一种化妆品用刺五加提取物,采用绿色环保的提取工艺得到一种抗氧化功能的植物提取物,具有良好的抗氧化效果,有利于缓解皮肤衰老,安全无刺激,可行性强。同时还提供一种护肤组合物,所述护肤组合物能够明显改善皮肤纹理,提升皮肤抗氧化能力。(The invention relates to the field of IPC A61K36/25, in particular to an acanthopanax extract for cosmetics and application thereof. The preparation method comprises the following steps: s1, collecting acanthopanax plants, drying the acanthopanax plants, and crushing; s2, adding the crushed acanthopanax plant into a first solvent for extraction to obtain an extracting solution; s3, carrying out reduced pressure concentration on the extracting solution, and adding a second solvent to obtain a suspension; and S4, extracting the suspension by using a third solvent to obtain a finished product of the acanthopanax senticosus extract. The invention provides an acanthopanax extract for cosmetics, which is a plant extract with an antioxidant function obtained by adopting a green and environment-friendly extraction process, has a good antioxidant effect, is beneficial to relieving skin aging, is safe and non-irritant, and has strong feasibility. Meanwhile, the skin care composition can obviously improve skin texture and improve the oxidation resistance of the skin.)

1. An acanthopanax extract for cosmetics is characterized by comprising the following preparation steps:

s1, collecting acanthopanax plants, drying the acanthopanax plants, and crushing;

s2, adding the crushed acanthopanax plant into a first solvent for extraction to obtain an extracting solution;

s3, carrying out reduced pressure concentration on the extracting solution, and adding a second solvent to obtain a suspension;

and S4, extracting the suspension by using a third solvent to obtain a finished product of the acanthopanax senticosus extract.

2. The Acanthopanax senticosus extract as claimed in claim 1, wherein the Acanthopanax senticosus plant comprises one or more of root of Acanthopanax senticosus, stem of Acanthopanax senticosus, leaf of Acanthopanax senticosus, fruit of Acanthopanax senticosus, flower of Acanthopanax senticosus, and seed of Acanthopanax senticosus.

3. The Acanthopanax senticosus extract for cosmetics according to claim 1 or 2, wherein the first solvent is a mixture of a small molecular alcohol and water; the volume ratio of the small molecular alcohol to the water is (60-98): (2-40).

4. The Acanthopanax senticosus extract for cosmetics according to any one of claims 1 to 3, wherein the extraction in the step S2 is specifically performed by adding the pulverized Acanthopanax senticosus plant into a first solvent, heating and refluxing for 1-10h, filtering, and collecting the filtrate to obtain an extract.

5. The Acanthopanax senticosus extract for cosmetics according to claim 4, wherein the temperature of the heating reflux in the step S2 is 40-80 ℃.

6. The Acanthopanax senticosus extract for cosmetics according to any one of claims 1 to 5, wherein the third solvent comprises one or more of methanol, ethanol, 1, 3-butanediol, ether, ethyl acetate, n-hexane, and chloroform.

7. The use of the Acanthopanax senticosus extract for cosmetics according to any one of claims 1 to 6, wherein the Acanthopanax senticosus extract is used in a skin care composition; the raw materials of the skin care composition comprise:

phase A: 2-19% of plant extract;

phase B: 0.1-5% of micromolecular polyalcohol, 0.1-1% of amino acid and the balance of water;

and C phase: 0.1-1% of vitamin derivative and 0.5-6% of emulsifier;

phase D: 0.1 to 0.5 percent of preservative;

phase E: 0.1-1% of essence;

wherein, the sum of the weight ratios of the phase A, the phase B, the phase C, the phase D and the phase E is 100 percent;

the plant extract comprises at least the Acanthopanax senticosus extract obtained according to any one of claims 1 to 6.

8. The use of Acanthopanax senticosus extract for cosmetics according to claim 7, wherein the small molecule polyol comprises one or more of glycerol, butylene glycol, pentylene glycol, and octylene glycol.

9. The use of Acanthopanax senticosus extract as claimed in claim 7 or 8, wherein the amino acids include alanine, arginine, aspartic acid, cysteine, glutamic acid, glycine, histidine, leucine, isoleucine, lysine, phenylalanine, 2-thioimidazole amino acids.

10. The use of Acanthopanax senticosus extract for cosmetics according to any one of claims 7-9, wherein the emulsifier comprises one or more of PEG-hydrogenated castor oil, glyceryl stearate, polydimethylsiloxane, stearyl alcohol, ceteareth, polyglyceryl-distearate, and ammonium acryloyldimethyltaurate/VP copolymer.

Technical Field

The invention relates to the field of IPC A61K36/25, in particular to an acanthopanax extract for cosmetics and application thereof.

Background

With the increase of age, the pace of life is accelerated and the air environment is worsened, free radicals continuously generated in the skin accelerate cell aging, the antioxidant defense capacity of the skin is reduced, and the problems of fine wrinkles, loose and rough skin, pigmentation spots and the like are frequently caused. Substances or compositions which are derived from natural plants and have the effects of moisturizing and nourishing have the advantages of high safety, low cost and the like, and become popular choices in the field of cosmetics nowadays. Chinese patent CN201510636787.5 discloses a moisturizing and skin-care facial mask liquid and a preparation method thereof, wherein the moisturizing and skin-care facial mask liquid is prepared by mixing components such as a thickening agent, an alcohol humectant, hyaluronic acid, an acanthopanax root extract and the like, and the moisturizing, water locking and whitening effects are achieved. Chinese patent CN201510396088.8 discloses a process for preparing an anti-skin-aging skin care lotion by utilizing alfalfa, which adopts a mode of combining ultrasonic-assisted extraction, microbial fermentation and ultrasonic-assisted alcohol extraction processes to extract components such as acanthopanax, polygonum multiflorum, gynostemma pentaphyllum, saffron and the like, thereby improving the anti-aging and beauty maintaining effects of the skin care lotion. In the prior art, the plant extract is applied to cosmetics, and (1) the activity of the extract is low, and the effect of the plant extract applied to the cosmetics is not ideal; (2) the high-activity plant extract is obtained by adopting a complicated extraction process, so that the popularization difficulty is high. Under the background, the research of a natural plant extract for cosmetics with high feasibility and obvious skin care effect becomes a problem to be solved in the field.

Disclosure of Invention

The invention provides the acanthopanax senticosus extract for cosmetics, solves the problems of low extraction efficiency and low activity of plant extracts in the prior art, and realizes the natural plant extract for cosmetics with high operation feasibility and obvious skin care effect.

In order to solve the above problems, the present invention provides a cosmetic acanthopanax senticosus extract, which comprises the following steps:

s1, collecting acanthopanax plants, drying the acanthopanax plants, and crushing;

s2, adding the crushed acanthopanax plant into a first solvent for extraction to obtain an extracting solution;

s3, carrying out reduced pressure concentration on the extracting solution, and adding a second solvent to obtain a suspension;

and S4, extracting the suspension by using a third solvent to obtain a finished product of the acanthopanax senticosus extract.

In some preferred embodiments, the Acanthopanax senticosus plant body comprises a combination of one or more of Acanthopanax senticosus root, Acanthopanax senticosus stem, Acanthopanax senticosus leaf, Acanthopanax senticosus fruit, Acanthopanax senticosus flower, and Acanthopanax senticosus seed.

Further preferably, the Acanthopanax senticosus plant body comprises root, stem, leaf and fruit of Acanthopanax senticosus. In the process of preparing the acanthopanax senticosus extract, the acanthopanax senticosus root, the acanthopanax senticosus stem, the acanthopanax senticosus leaf and the acanthopanax senticosus fruit are respectively subjected to S1-S4 operations, and finally the obtained acanthopanax senticosus plant body extracts are combined to obtain the acanthopanax senticosus extract with high safety and high moisturizing and moistening effects.

In some preferred embodiments, the size of the pulverized acanthopanax senticosus extract in the S1 step is 20-600 mesh.

In some preferred embodiments, the first solvent is a mixture of a small molecule alcohol and water; the volume ratio of the small molecular alcohol to the water is (60-98): (2-40), preferably 75: 2.

further preferably, the small molecule alcohol comprises one or more of methanol, ethanol, 1-propanol, 1-butanol, 1-pentanol, 1-hexanol, 1-octanol, 2-methyl-1-propanol, cyclopentanol and ethylene glycol.

In some preferred embodiments, the extracting operation is specifically that the pulverized acanthopanax plant is added into a first solvent, heated and refluxed for extraction for 1-10 hours, and filtered to collect filtrate, namely the extracting solution.

More preferably, the extraction operation specifically comprises adding the pulverized acanthopanax senticosus plant into a first solvent, heating and refluxing for 1-3h, and filtering to obtain a first filter residue and a first filtrate; adding the filter residue I into a first solvent, heating and refluxing for 1-3h, and filtering to obtain a filter residue II and a filtrate II; adding the filter residue II into the first solvent, heating and refluxing for 1-3h, and filtering to obtain filter residue III and filtrate III; and mixing the first filtrate, the second filtrate and the third filtrate to obtain an extracting solution.

In some preferred embodiments, the temperature of the heating reflux in the S2 step is 40 to 80 ℃.

In some preferred embodiments, the vacuum degree of the reduced pressure concentration operation is 0.01 to 0.1 MPa.

In some preferred embodiments, the second solvent is water; the volume ratio of the extracting solution to the water is 1: (0.5-2).

In some preferred embodiments, the third solvent comprises a combination of one or more of methanol, ethanol, 1, 3-butanediol, ether, ethyl acetate, n-hexane, chloroform; further preferred is ethyl acetate.

Acanthopanax root: the Latin scientific name Acanthopanaxsenicosus (Rupr. Maxim.) Harms is a wild plant of Araliaceae and Araliaceae, and has high medicinal value; the plant contains rich nutrient components such as glycosides, flavones, polysaccharides, vitamins, minerals, amino acids, etc., and can effectively remove free radicals from human body, inhibit aging, enhance immunity, regulate energy metabolism, and improve skin condition. According to the invention, a large number of experiments prove that the acanthopanax extract with high purity and high free radical inhibition rate can be obtained by adopting a specific preparation method. Especially, by adopting the process flows of crushing, alcohol extraction, concentration and extraction, the nutrient components in the acanthopanax plant can be leached to the maximum extent, and meanwhile, the extract has extremely high free radical scavenging capacity. Further, the acanthopanax senticosus plant is extracted by using a 75% ethanol aqueous solution, and then an organic solvent (especially ethyl acetate) is used for extracting the extract, so that the obtained acanthopanax senticosus extract has strong capability of removing free radicals and higher medium compatibility, can play a synergistic role together with small molecular polyol, amino acid and the like when being applied to a skin care composition, effectively improves skin texture, fully plays an antioxidation role and activates the skin elasticity state.

The second aspect of the invention provides an application of an acanthopanax extract for cosmetics, wherein the acanthopanax extract is applied to a skin care composition and accounts for 1-15% of the total mass of the skin care composition; the raw materials of the skin care composition comprise:

phase A: 2-19% of plant extract;

phase B: 0.1-5% of micromolecular polyalcohol, 0.1-1% of amino acid and the balance of water;

and C phase: 0.1-1% of vitamin derivative and 0.5-6% of emulsifier;

phase D: 0.1 to 0.5 percent of preservative;

phase E: 0.1 to 1 percent of essence.

Wherein the sum of the weight ratios of the phase A, the phase B, the phase C, the phase D and the phase E is 100 percent.

The plant extract at least comprises the acanthopanax senticosus extract.

In some preferred embodiments, the plant extract comprises a combination of one or more of acanthopanax senticosus extract, aloe barbadensis extract and olive fruit extract, purslane extract, centella asiatica extract, ginseng extract, jojoba extract, and pueraria lobata extract.

Further preferably, the plant extracts include acanthopanax senticosus extract, aloe barbadensis extract and olive fruit extract; the extract comprises radix Acanthopanacis Senticosi extract, Aloe Barbadensis Miller extract and fructus Canarii albi extract at weight ratio of (2-15): (0.1-2%): (0.5-5).

The small molecular polyol comprises one or more of glycerol, butanediol, pentanediol and octanediol.

The small molecular polyol comprises glycerol and caprylyl glycol; the weight ratio of the glycerol to the caprylyl glycol is (7-12): 1.

the amino acid comprises one or more of alanine, arginine, aspartic acid, semi-fat ammonia acid, glutamic acid, glycine, histidine, leucine, isoleucine, lysine, phenylalanine, 2-thioimidazole amino acid; 2-Thioimidazolamino is preferred.

As examples of vitamin derivatives, but not limited to, tocopherol acetate.

In some preferred embodiments, the emulsifier comprises a combination of one or more of PEG-hydrogenated castor oil, glyceryl stearate, dimethicone, stearyl alcohol, ceteareth, polyglyceryl-distearate, ammonium acryloyldimethyl taurate/VP copolymer.

Examples of preservatives include, but are not limited to, phenoxyethanol, caprylhydroxamic acid, ethylhexylglycerin, p-hydroxyacetophenone.

In some preferred embodiments, the skin care composition is prepared by a process comprising:

(1) adding the raw material of phase B into a water phase pot, and mixing at 80-85 deg.C;

(2) adding the C-phase raw material into an emulsifying pot, and uniformly mixing at 80-85 ℃; then pumping the C-phase raw material mixture in the emulsifying pot into a water phase pot, and uniformly stirring;

(3) cooling the water phase pot to 40-50 deg.C, adding phase A raw materials, mixing, cooling to 38-42 deg.C, adding phase D and phase E raw materials, mixing, filtering, and discharging to obtain the final product.

The invention further discovers that when the acanthopanax senticosus extract is applied to the skin care composition, the acanthopanax senticosus extract can quickly improve textures in a short period when being added in an amount of 1-15 wt%, inhibit the generation of fine lines and dark lines and enable a loose skin barrier to regain elasticity. Further, the acanthopanax extract prepared by the invention is selected as a plant extract to act together with the aloe vera extract and the olive fruit extract, and 2-thioimidazole amino is selected, the skin texture of the face can be obviously improved after the acanthopanax extract is used for 4 weeks, the change rate of the skin texture of 45% of subjects is more than 25% after the acanthopanax extract is used for 4 weeks, the acanthopanax extract can be used for well improving the skin aging problem, the skin oxidation resistance is improved from the inner source, the skin barrier is effectively repaired, and the skin defense capability is improved.

In some preferred embodiments, the skin care composition can be used to prepare a smoothing toner, a serum, an emulsion, a cream, and the like.

Has the advantages that:

the invention provides an acanthopanax extract for cosmetics, which is a plant extract with an antioxidant function obtained by adopting a green and environment-friendly extraction process, has a good antioxidant effect, is beneficial to relieving skin aging, is safe and non-irritant, and has strong feasibility. Meanwhile, the application of the acanthopanax senticosus extract in cosmetics is provided, so that the skin care composition is obtained, the skin texture can be obviously improved, the oxidation resistance of the skin is improved, the skin care composition can be widely used for smoothing toner, essence, emulsion or cream and the like, and the application prospect is wide.

Detailed Description

Example 1.

The embodiment provides an acanthopanax extract for cosmetics, which comprises the following preparation steps:

s1, collecting acanthopanax plants, drying the acanthopanax plants, and crushing;

s2, adding the crushed acanthopanax plant into a first solvent for extraction to obtain an extracting solution;

s3, carrying out reduced pressure concentration on the extracting solution, and adding a second solvent to obtain a suspension;

and S4, extracting the suspension by using a third solvent, and removing the third solvent under reduced pressure to obtain a finished product of the acanthopanax senticosus extract.

The Acanthopanax senticosus plant comprises root part, stem part, leaf part and fruit of Acanthopanax senticosus. In the process of preparing the acanthopanax senticosus extract, the acanthopanax senticosus root, the acanthopanax senticosus stem, the acanthopanax senticosus leaf and the acanthopanax senticosus fruit are respectively subjected to S1-S4 operations, and finally the obtained acanthopanax senticosus plant body extracts are combined to obtain the acanthopanax senticosus extract.

The particle size of the pulverized acanthopanax senticosus extract in the step S1 is 80 meshes.

The first solvent is a mixture of small molecular alcohol and water; the volume ratio of the small molecular alcohol to the water is 75: 2.

the small molecular alcohol is ethanol.

The extraction operation in the step S2 is specifically that the crushed acanthopanax senticosus plant is added into a first solvent, heated and refluxed for 2 hours, and filtered by a 200-mesh sieve to obtain a first filter residue and a first filtrate; adding the filter residue I into a first solvent, heating and refluxing for 2h, and filtering by a 200-mesh sieve to obtain a filter residue II and a filtrate II; adding the filter residue II into the first solvent, heating and refluxing for 2h, and filtering by a 200-mesh sieve to obtain filter residue III and filtrate III; and mixing the first filtrate, the second filtrate and the third filtrate to obtain an extracting solution.

The heating reflux temperature in the step S2 was 60 ℃.

In the step S2, the weight ratio of the acanthopanax senticosus plant body to the first solvent, the weight ratio of the first filter residue to the first solvent, and the weight ratio of the second filter residue to the first solvent are both 1: 10.

the vacuum degree of the decompression concentration operation is 0.05 MPa.

The second solvent is water; the volume ratio of the extracting solution to the water is 1: 1.

the third solvent is ethyl acetate; the weight ratio of the third solvent to the suspension is 1: 1.

acanthopanax root: the latin scientific name acanthopanaxsentiosus (rupr. maxim.) Harms; the acanthopanax root, the acanthopanax stem, the acanthopanax leaf and the acanthopanax fruit are all derived from a fortune seedling planting base of the lotus seed.

Example 2.

The embodiment provides an application of an acanthopanax senticosus extract for cosmetics, wherein the acanthopanax senticosus extract is applied to a skin care composition, and the skin care composition comprises the following raw materials:

phase A: 12% of plant extract;

phase B: 3.3 percent of micromolecular polyalcohol, 0.3 percent of amino acid and the balance of water;

and C phase: 0.5% of vitamin derivative and 2.3% of emulsifier;

phase D: 0.3 percent of preservative;

phase E: 0.1 percent of essence.

Wherein the sum of the weight ratios of the phase A, the phase B, the phase C, the phase D and the phase E is 100 percent.

The plant extract is selected from radix Acanthopanacis Senticosi extract, Aloe Barbadensis Miller extract and fructus Canarii albi extract; the extract comprises radix Acanthopanacis Senticosi extract, Aloe Barbadensis Miller extract and fructus Canarii albi extract at a weight ratio of 10: 0.5: 1.5.

the micromolecular polyalcohol is glycerol and caprylyl glycol; the weight ratio of the glycerol to the caprylyl glycol is 10: 1.

the amino acid is 2-thioimidazole amino acid, and the CAS number is 497-30-3.

The vitamin derivative is tocopherol acetate.

The emulsifier is PEG-40 hydrogenated castor oil, glyceryl stearate, polydimethylsiloxane and stearyl alcohol; the mass ratio of PEG-40 hydrogenated castor oil, glycerol stearate, polydimethylsiloxane to stearyl alcohol is 0.15:1:1:0.1.

The preservative is phenoxyethanol; the essence is from Jiangxi Baolin natural spice limited company.

The acanthopanax senticosus extract is the acanthopanax senticosus extract provided by the embodiment 1; the Aloe vera (L.) burm. f.) extract and the olive fruit (Canarium album (Lour.) Raeusch.) extract were both from chuxiang (shanghai) biotechnology limited.

The skin care composition is prepared by the steps of:

(1) adding the raw material of phase B into a water phase pot, and uniformly mixing at 85 ℃;

(2) adding the C-phase raw material into an emulsifying pot, and uniformly mixing at 85 ℃; then pumping the C-phase raw material mixture in the emulsifying pot into a water phase pot, and uniformly stirring;

(3) cooling the water phase pot to 45 deg.C, adding phase A raw materials, mixing, cooling to 40 deg.C, adding phase D and phase E raw materials, mixing, filtering with 200 mesh sieve, and discharging to obtain the final product.

Example 3.

The embodiment provides an application of an acanthopanax senticosus extract for cosmetics, wherein the acanthopanax senticosus extract is applied to a skin care composition, and the skin care composition comprises the following raw materials:

phase A: 8% of plant extract;

phase B: 3.3 percent of micromolecular polyalcohol, 0.3 percent of amino acid and the balance of water;

and C phase: 0.5% of vitamin derivative and 2.3% of emulsifier;

phase D: 0.3 percent of preservative;

phase E: 0.1 percent of essence.

Wherein the sum of the weight ratios of the phase A, the phase B, the phase C, the phase D and the phase E is 100 percent.

The plant extract is selected from radix Acanthopanacis Senticosi extract, Aloe Barbadensis Miller extract and fructus Canarii albi extract; the extract comprises radix Acanthopanacis Senticosi extract, Aloe Barbadensis Miller extract and fructus Canarii albi extract at weight ratio of 6: 0.5: 1.5.

the micromolecular polyalcohol is glycerol and caprylyl glycol; the weight ratio of the glycerol to the caprylyl glycol is 10: 1.

the amino acid is 2-thioimidazole amino acid, and the CAS number is 497-30-3.

The vitamin derivative is tocopherol acetate.

The emulsifier is PEG-40 hydrogenated castor oil, glyceryl stearate, polydimethylsiloxane and stearyl alcohol; the mass ratio of PEG-40 hydrogenated castor oil, glycerol stearate, polydimethylsiloxane to stearyl alcohol is 0.15:1:1:0.1.

The preservative is phenoxyethanol; the essence is from Jiangxi Baolin natural spice limited company.

The acanthopanax senticosus extract is the acanthopanax senticosus extract provided by the embodiment 1; the Aloe vera (L.) burm. f.) extract and the olive fruit (Canarium album (Lour.) Raeusch.) extract were both from chuxiang (shanghai) biotechnology limited.

The skin care composition is prepared by the steps of:

(1) adding the raw material of phase B into a water phase pot, and uniformly mixing at 85 ℃;

(2) adding the C-phase raw material into an emulsifying pot, and uniformly mixing at 85 ℃; then pumping the C-phase raw material mixture in the emulsifying pot into a water phase pot, and uniformly stirring;

(3) cooling the water phase pot to 45 deg.C, adding phase A raw materials, mixing, cooling to 40 deg.C, adding phase D and phase E raw materials, mixing, filtering with 200 mesh sieve, and discharging to obtain the final product.

Example 4.

The embodiment provides an application of an acanthopanax senticosus extract for cosmetics, wherein the acanthopanax senticosus extract is applied to a skin care composition, and the skin care composition comprises the following raw materials:

phase A: 2.001% of plant extract;

phase B: 3.3 percent of micromolecular polyalcohol, 0.3 percent of amino acid and the balance of water;

and C phase: 0.5% of vitamin derivative and 2.3% of emulsifier;

phase D: 0.3 percent of preservative;

phase E: 0.1 percent of essence.

Wherein the sum of the weight ratios of the phase A, the phase B, the phase C, the phase D and the phase E is 100 percent.

The plant extract is selected from radix Acanthopanacis Senticosi extract, Aloe Barbadensis Miller extract and fructus Canarii albi extract; the extract comprises radix Acanthopanacis Senticosi extract, Aloe Barbadensis Miller extract and fructus Canarii albi extract at a weight ratio of 0.001: 0.5: 1.5.

the micromolecular polyalcohol is glycerol and caprylyl glycol; the weight ratio of the glycerol to the caprylyl glycol is 10: 1.

the amino acid is 2-thioimidazole amino acid, and the CAS number is 497-30-3.

The vitamin derivative is tocopherol acetate.

The emulsifier is PEG-40 hydrogenated castor oil, glyceryl stearate, polydimethylsiloxane and stearyl alcohol; the mass ratio of PEG-40 hydrogenated castor oil, glycerol stearate, polydimethylsiloxane to stearyl alcohol is 0.15:1:1:0.1.

The preservative is phenoxyethanol; the essence is from Jiangxi Baolin natural spice limited company.

The acanthopanax senticosus extract is the acanthopanax senticosus extract provided by the embodiment 1; the Aloe vera (L.) burm. f.) extract and the olive fruit (Canarium album (Lour.) Raeusch.) extract were both from chuxiang (shanghai) biotechnology limited.

The skin care composition is prepared by the steps of:

(1) adding the raw material of phase B into a water phase pot, and uniformly mixing at 85 ℃;

(2) adding the C-phase raw material into an emulsifying pot, and uniformly mixing at 85 ℃; then pumping the C-phase raw material mixture in the emulsifying pot into a water phase pot, and uniformly stirring;

(3) cooling the water phase pot to 45 deg.C, adding phase A raw materials, mixing, cooling to 40 deg.C, adding phase D and phase E raw materials, mixing, filtering with 200 mesh sieve, and discharging to obtain the final product.

Performance test method

1. Free radical scavenging ability test:

(1) DPPH method

Accurately weighing 6mg of DPPH (1, 1-diphenyl-2-trinitrophenylhydrazine), dissolving with 95% VOL ethanol water solution, dissolving with the aid of ultrasonic wave, then fixing the volume to a 50mL volumetric flask, and diluting with 95% VOL ethanol water solution until the DPPH concentration is 3mg/100mL to obtain DPPH mother liquor; the DPPH mother liquor is stored in a refrigerator at 0-4 ℃ in a dark place.

Dissolving 2g of the acanthopanax senticosus extract obtained in the example 1 in deionized water to obtain a sample to be detected, adding an equal volume of DPPH mother liquor into the sample to be detected, carrying out a light-shielding reaction at 26 ℃ for 30min, measuring the absorbance at 525nm, and calculating the IC50 (half inhibition concentration); see table 1.

At the same time, 2g of ascorbic acid was used as a control group, and the above-mentioned operation was repeated to determine the half inhibitory concentration of ascorbic acid against DPPH.

The lower the semi-inhibitory concentration to DPPH, the greater the free radical scavenging ability of the sample.

(2) ABTS method

Preparing ABTS (2, 2-diazo-bis (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt) aqueous solution with the concentration of 7mmol/L, mixing the ABTS aqueous solution with 2.45mmol/L potassium persulfate aqueous solution according to the weight ratio of 1:1, stirring the mixture for 16 hours in a dark place, and then adding PBS solution (phosphate buffer solution) to dilute the mixture until the light absorption value at 734nm is 0.70 +/-0.02 to obtain ABTS mother liquor.

Diluting the Acanthopanax senticosus extract of example 1 with 50% VOL petroleum ether ethanol solution to 3. mu.g/mL, 6. mu.g/mL, 13. mu.g/mL, 25. mu.g/mL, 50. mu.g/mL; mixing the samples with the concentration gradient with ABTS mother liquor respectively, wherein the volume ratio of the samples to the ABTS mother liquor is 1: 20; after mixing, the mixture was placed at 26 ℃ in the dark for 6min, and the absorbance at 734nm was measured to calculate the ABTS clearance D, see Table 2.

D ═ 100% (1-a1/a0) ×, where a1 is the absorbance of the ABTS mother liquor and a0 is the absorbance measured after mixing the ABTS mother liquor with the sample.

The higher the ABTS clearance rate D, the stronger the free radical scavenging ability and the oxidation resistance of the sample.

2. Skin care effect:

180 subjects were screened and divided into 3 groups of 60 persons each. Selecting the left and right cheekbones of the face of a subject, the left and right cheeks at the intersection of the nose tip and the pupil, and the left and right sides at the intersection of the temple and the mouth corner as test areas, firstly, measuring the skin texture of the test part before applying the cosmetics by a technician by using a visia facial image analyzer, measuring five times at each point, and taking an average value. The subjects continued to use the skin care products of examples 2-4 for four weeks (1 each day in the morning and evening), and again tested for skin texture. Counting the skin texture degree value measured at each time of the experimental part of the subject, and analyzing the change rule of the skin texture, thereby determining the effect of the skin care product of the embodiment 2-4 on improving the skin texture; see table 3.

Performance test data

Table 1.

Sample (I)	IC50(μg/mL)
		Example 1	0.31
Ascorbic acid	2.58

Table 2.

Concentration of Acanthopanax senticosus extract (. mu.g/mL)	ABTS clearance D (%)
		3	30.2
6	39.5
		13	51.4
25	67.3
		50	84.1

Table 3.

The combination of the results shows that the acanthopanax extract provided by the invention has excellent free radical scavenging capacity, the half inhibition concentration of DPPH can be as low as 0.31 mu g/mL, and the free radical scavenging capacity is superior to that of a positive control ascorbic acid; meanwhile, the acanthopanax senticosus extract has strong ABTS removing capacity, and the ABTS removing rate is up to more than 80%; the subjects using the skin care compositions of examples 2 to 3 showed significant improvement in skin texture after 4 weeks, and it was demonstrated that the acanthopanax senticosus extract of the present invention added to the skin care composition could sufficiently exert the antioxidant activity and improve the skin condition.