阅读说明：本技术 转阀及pcr芯片 (Rotary valve and PCR chip ) 是由 蒋兴宇 郑锦涛 牟磊 于 2021-08-03 设计创作，主要内容包括：本发明公开了一种转阀及PCR芯片,包括密封件和支撑件。其中,密封件能够发生弹性变形,密封件上设置有连接孔和阀通道。支撑件采用硬质材料制成,支撑件包括连接部和支撑部,连接部固定于支撑部,支撑部远离连接部的一端插设于连接孔。具有一定弹性的密封件,在应用于PCR芯片时,能够有效改善密封性,提高实验的准确度。同时,支撑件通过插接的方式与密封件相配合,能够为密封件提供有效的支撑,确保了转阀能够正常使用。由此,在实际使用时转阀无需再与其它元件进行配合,从而使得转阀在确保具有良好密封性的同时,精简了自身结构。(The invention discloses a rotary valve and a PCR chip. Wherein, the sealing member can take place elastic deformation, is provided with connecting hole and valve passageway on the sealing member. The supporting piece is made of hard materials and comprises a connecting portion and a supporting portion, the connecting portion is fixed on the supporting portion, and one end, far away from the connecting portion, of the supporting portion is inserted into the connecting hole. The sealing element with certain elasticity can effectively improve the sealing property and improve the accuracy of an experiment when being applied to a PCR chip. Meanwhile, the supporting piece is matched with the sealing piece in an inserting mode, effective support can be provided for the sealing piece, and normal use of the rotary valve is guaranteed. Therefore, the rotary valve does not need to be matched with other elements in actual use, so that the rotary valve is simple in structure while good sealing performance is ensured.)

1. A rotary valve, comprising:

the sealing element can be elastically deformed and is provided with a connecting hole and a valve channel;

the supporting part comprises a connecting part and a supporting part, the connecting part is fixed on the supporting part, and one end, far away from the connecting part, of the supporting part is inserted into the connecting hole.

2. The rotary valve according to claim 1, wherein the support member further comprises a torsion portion fixed to the connecting portion, the torsion portion being provided with a torsion groove.

3. The rotary valve according to claim 1, wherein the connection portion is provided with a fool-proof groove for indicating a position of the connection portion.

A PCR chip, comprising:

the chip comprises a chip main body, wherein a processing cavity and an amplification cavity are arranged in the chip main body, a first accommodating groove, a second accommodating groove and an air outlet are arranged on the chip main body, the first accommodating groove is positioned between the processing cavity and the amplification cavity and is communicated with the processing cavity and the amplification cavity, and the second accommodating groove is positioned between the amplification cavity and the air outlet and is communicated with the amplification cavity and the air outlet;

at least two rotary valves according to any one of claims 1 to 3, one of said rotary valves being disposed in said first receiving chamber and the other of said rotary valves being disposed in said second receiving chamber, said rotary valve at said first receiving chamber being a first rotary valve and said rotary valve at said second receiving chamber being a second rotary valve, said valve passage of said first rotary valve rotated to a first predetermined position communicating said process chamber with said amplification chamber, said valve passage of said second rotary valve rotated to a second predetermined position communicating said amplification chamber with said gas outlet, said first rotary valve rotated from said first predetermined position maintaining said amplification chamber sealed with said second rotary valve rotated from said second predetermined position.

5. The PCR chip of claim 4, wherein the PCR chip comprises 3 rotary valves, the chip body further comprises a first air passage, a second air passage and a plurality of feeding chambers independent of each other, the chip body further comprises a third receiving chamber, the third receiving chamber is located between the feeding chambers and the processing chambers, the third receiving chamber is communicated with the processing chambers and the feeding chambers, the third receiving chamber is provided with one rotary valve, the rotary valve in the third receiving chamber is a third rotary valve, the valve passage of the third rotary valve rotated to a third preset position enables the feeding chambers to be communicated with the processing chambers, the first air passage is communicated with the processing chambers and the first receiving chamber, the second air passage is communicated with the first receiving chamber and the air outlet, and the valve passage of the first rotary valve rotated to a fourth preset position enables the first air passage to be communicated with the second air passage The method is simple.

6. The PCR chip of claim 5, further comprising a negative pressure air pump disposed at the air outlet.

7. The PCR chip of claim 6, wherein a buffer chamber is further disposed in the chip body, and the buffer chamber is located between the air outlet and the second receiving groove and is communicated with the air outlet and the second receiving groove.

8. The PCR chip of claim 5, further comprising a positive pressure air pump, wherein the chip body is further provided with an air blowing port, the air blowing port is communicated with the second air passage, and the positive pressure air pump is arranged at the air blowing port.

9. The PCR chip of claim 5, wherein a plurality of collection holes are further disposed in the chip body and are in communication with the amplification chamber, and the collection holes are independent of each other.

10. The PCR chip of claim 9, wherein the aperture of the collection well gradually decreases away from the amplification chamber.

Technical Field

The invention relates to the field of microfluidic detection, in particular to a rotary valve and a PCR chip.

Background

In the related art, the rotary valve in the PCR chip is usually made of a hard material, and when the rotary valve is actually used, the sealing performance is often poor, and in order to improve the sealing performance, a sealing ring is additionally added, so that the internal structure of the PCR chip is complicated.

Disclosure of Invention

The present invention is directed to solving at least one of the problems of the prior art. Therefore, the invention provides a rotary valve which can ensure the sealing performance while ensuring the simplification of the structure.

The invention also provides a PCR chip with the rotary valve.

An embodiment of the first aspect of the invention provides a rotary valve, comprising:

the sealing element can be elastically deformed and is provided with a connecting hole and a valve channel;

the supporting part comprises a connecting part and a supporting part, the connecting part is fixed on the supporting part, and one end, far away from the connecting part, of the supporting part is inserted into the connecting hole.

The rotary valve provided by the embodiment of the invention has at least the following technical effects:

the sealing element with certain elasticity can effectively improve the sealing property and improve the accuracy of an experiment when being applied to a PCR chip. Meanwhile, the supporting piece is matched with the sealing piece in an inserting mode, effective support can be provided for the sealing piece, and normal use of the rotary valve is guaranteed. Therefore, the rotary valve does not need to be matched with other elements in actual use, so that the rotary valve is simple in structure while good sealing performance is ensured.

According to the rotary valve of some embodiments of the present invention, the support member further includes a torsion portion fixed to the connection portion, and the torsion portion is provided with a torsion groove.

According to the rotary valve of some embodiments of the present invention, the connection portion is provided with a fool-proof groove for indicating a position of the connection portion.

In a second aspect, embodiments of the present invention provide a PCR chip, including:

the chip comprises a chip main body, wherein a processing cavity and an amplification cavity are arranged in the chip main body, a first accommodating groove, a second accommodating groove and an air outlet are arranged on the chip main body, the first accommodating groove is positioned between the processing cavity and the amplification cavity and is communicated with the processing cavity and the amplification cavity, and the second accommodating groove is positioned between the amplification cavity and the air outlet and is communicated with the amplification cavity and the air outlet;

two rotary valves according to the above first aspect of the present invention, one of the rotary valves is disposed in the first receiving chamber, the other rotary valve is disposed in the second receiving chamber, the rotary valve at the first receiving chamber is a first rotary valve, the rotary valve at the second receiving chamber is a second rotary valve, the valve passage of the first rotary valve rotated to a first preset position connects the processing chamber and the amplification chamber, the valve passage of the second rotary valve rotated to a second preset position connects the amplification chamber and the air outlet, and the first rotary valve rotated from the first preset position and the second rotary valve rotated from the second preset position keep the amplification chamber sealed.

The PCR chip provided by the embodiment of the invention at least has the following technical effects:

by adopting the rotary valve, the sealing performance of the amplification cavity in the detection process is effectively optimized, and the structure of the PCR chip is simplified.

According to the PCR chip of some embodiments of the invention, the number of the rotary valves is three, the chip body is also provided with a first air passage, a second air passage and a plurality of mutually independent feeding cavities, the chip main body is also provided with a third containing groove which is positioned between the feeding cavity and the processing cavity, the third containing groove is communicated with the processing cavity and each feeding cavity, the third containing groove is provided with the rotary valve, the rotary valve at the third containing groove is taken as a third rotary valve, the valve channel of the third rotary valve which rotates to a third preset position enables the feeding cavity to be communicated with the processing cavity, the first air passage is communicated with the processing cavity and the first accommodating groove, the second air passage is communicated with the first accommodating groove and the air outlet, and the valve passage of the first rotary valve which rotates to a fourth preset position enables the first air passage to be communicated with the second air passage.

The PCR chip according to some embodiments of the present invention further comprises a negative pressure air pump disposed at the air outlet.

According to the PCR chip of some embodiments of the present invention, a buffer chamber is further disposed in the chip body, and the buffer chamber is located between the air outlet and the second receiving groove and is communicated with the air outlet and the second receiving groove.

According to some embodiments of the invention, the PCR chip further comprises a positive pressure air pump, the chip main body is further provided with an air blowing port, the air blowing port is communicated with the second air passage, and the positive pressure air pump is arranged at the air blowing port.

According to the PCR chip of some embodiments of the invention, a plurality of collecting holes communicated with the amplification cavity are further arranged in the chip body, and the collecting holes are independent from each other.

According to the PCR chip of some embodiments of the present invention, the aperture of the collection well gradually decreases toward a direction away from the amplification chamber.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is a schematic perspective view of a rotary valve in an embodiment of the present invention;

FIG. 2 is an exploded view of a rotary valve according to an embodiment of the present invention;

FIG. 3 is a schematic perspective view of a PCR chip according to an embodiment of the present invention;

FIG. 4 is a schematic perspective view of a PCR chip according to an embodiment of the present invention.

Reference numerals: the first rotary valve 101, the second rotary valve 102, the third rotary valve 103, the sealing member 110, the connection hole 111, the valve passage 112, the support member 120, the connection portion 121, the support portion 122, the torsion portion 123, the torsion groove 124, the fool-proof groove 125, the chip body 200, the first receiving groove 201, the second receiving groove 202, the third receiving groove 203, the processing chamber 210, the amplification chamber 220, the collection hole 221, the gas outlet 230, the loading chamber 240, the liquid inlet 241, the first gas passage 250, the second gas passage 260, the buffer chamber 270, and the EP tube 300.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.

In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, the meaning of a plurality of means is one or more, the meaning of a plurality of means is two or more, and larger, smaller, larger, etc. are understood as excluding the number, and larger, smaller, inner, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

A rotary valve according to an embodiment of the present invention will be described below with reference to fig. 1 and 2.

A rotary valve according to an embodiment of the first aspect of the present invention includes a sealing member 110 and a support member 120.

Wherein, the sealing member 110 is elastically deformable, and the sealing member 110 is provided with a connection hole 111 and a valve passage 112. The supporting member 120 includes a connecting portion 121 and a supporting portion 122, the connecting portion 121 is fixed to the supporting portion 122, and one end of the supporting portion 122 far from the connecting portion 121 is inserted into the connecting hole 111.

Specifically, the supporter 120 includes a connection part 121 and at least one support part 122. The connecting portion 121 and the supporting portion 122 are both cylindrical, one end surface of the supporting portion 122 is fixed on the bottom surface of the connecting portion 121, and the axis of the connecting portion 121 is parallel to the axis of the supporting portion 122. The supporter 120 may be made of a hard material such as aluminum alloy, stainless steel, etc. by machining, integral injection molding, 3D printing, etc., so that it has certain rigidity and strength.

The sealing member 110 is made of flexible material such as PDMS or silicone, and is formed by integral injection molding or 3D printing, so that it has certain elasticity. The sealing member 110 is in a circular truncated cone shape, a cylindrical shape or a shape of splicing a circular truncated cone and a cylindrical shape, at least one connecting hole 111 is formed in the bottom surface of one side of the sealing member, the number of the connecting holes is the same as that of the supporting parts 122, and the two positions are in one-to-one correspondence. The hole diameter of the communication hole is slightly smaller than the diameter of the support portion 122 so that the support portion 122 can be inserted into the communication hole with interference. Thus, the support member 120 is fixed to the seal member 110 by the interfitting of the support portion 122 and the communication hole, and at this time the axis of the connecting portion 121 is collinear with the axis of the seal member 110. When a torque is applied to the support member 120 in the axial direction, the support member 120 can drive the sealing member 110 to synchronously rotate in the axial direction.

Preferably, the supporting portion 122 and the connecting hole 111 are both provided with a plurality of supporting portions, so as to ensure that the supporting member 120 does not rotate with respect to the sealing member 110, avoid a slip between the supporting portion and the sealing member, and ensure that the supporting member 120 can effectively drive the sealing member 110 to rotate synchronously.

Meanwhile, a valve passage 112 is provided inside the sealing member 110. The valve passage 112 has at least two openings on the side surface of the sealing member 110, that is, when the rotary valve is a two-way valve, the valve passage 112 has two openings on the side surface of the sealing member 110; when the rotary valve is a three-way valve, etc., the valve passage 112 has three or more openings on the side surface of the sealing member 110, and the number of the openings is adaptively changed according to the type of the rotary valve.

In a specific use process, the rotary valve mounted on the chip body 200 of the PCR chip will be in contact with the chip body 200 of the PCR chip through the sealing member 110. It will be understood that the sealing member 110 having a certain elasticity is closely attached to the groove wall of the container provided on the chip body 200, thereby securing the sealability of the rotary valve. When the sealing member 110 is required to rotate to change the object connected to the valve passage 112, a torque is applied to the supporting member 120, and the supporting member 120 drives the sealing member 110 to rotate. The plug-in fit between the support member 120 and the sealing member 110 also effectively simplifies the structure of the rotary valve.

In conclusion, the design simplifies the specific structure of the rotary valve while ensuring good sealing performance of the rotary valve.

In some embodiments of the present invention, the supporting member 120 further includes a torsion portion 123, the torsion portion 123 is fixed to the connecting portion 121, and the torsion portion 123 is provided with a torsion groove 124.

Specifically, the supporting member 120 further includes a torsion portion 123, and the torsion portion 123 is used for cooperating with an output end of the driving structure, so that the driving member can drive the connecting portion 121 to drive the supporting portion 122 to rotate. The torsion portion 123 is fixed to the bottom surface of the connecting portion 121 remote from the support portion 122, and is also cylindrical. The torsion groove 124 is formed in the bottom face of the torsion portion 123 on the side away from the connecting portion 121, the torsion groove 124 can be in a cross shape or a straight shape, and the depth, the length and the width of the torsion groove 124 are matched with the end portion of the output end of the driving structure, so that the end portion of the output end of the driving structure can be embedded into the torsion groove 124, and the torque output to the connecting portion 121 is achieved by applying acting force to the groove wall of the torsion groove 124. The driving structure can be a rotary air cylinder or a rotary motor and the like.

It can be understood that the torsion portion 123 and the torsion groove 124 disposed thereon are configured such that the driving structure can indirectly apply a torque to the supporting portion 122, and the operating state of the driving structure can be preset in advance, such that the rotation angle of the rotary valve can be automatically controlled, thereby improving the degree of automation.

In some embodiments of the present invention, the connection portion 121 is provided with a fool-proof groove 125, and the fool-proof groove 125 is used for indicating the position of the connection portion 121.

Specifically, since the openings of the valve passage 112 provided on the side of the sealing member 110 need to communicate with the corresponding cavities, the mounting direction of the rotary valve needs to be confirmed when the rotary valve is mounted on the chip body 200 of the PCR chip. To improve the positioning of the assembly, a fool-proof groove 125 is provided on the connection portion 121.

The fool-proof groove 125 is provided at a side of the connection portion 121 far from the support portion 122, and at an edge of the connection portion 121, so that it is easy for an installer to find. The effect of preventing slow-witted groove 125 is assistance-localization real-time, through setting up to prevent slow-witted groove 125, the staff can discern correct installation direction rapidly when will changeing the valve and install the relevant position on chip main part 200 to installation effectiveness has been improved.

A PCR chip according to an embodiment of the present invention is described below with reference to FIGS. 3 and 4.

The PCR chip according to the second embodiment of the present invention comprises a chip body 200 and two rotary valves according to the first embodiment of the present invention.

The chip body 200 is provided with a processing cavity 210 and an amplification cavity 220, the chip body is provided with a first accommodating groove 201, a second accommodating groove 202 and an air outlet 230, the first accommodating groove 201 is located between the processing cavity 210 and the amplification cavity 220 and is communicated with the processing cavity 210 and the amplification cavity 220, and the second accommodating groove 202 is located between the amplification cavity 220 and the air outlet 230 and is communicated with the amplification cavity 220 and the air outlet 230. One rotary valve is arranged in the first containing groove 201, the other rotary valve is arranged in the second containing groove 202, the rotary valve at the first containing groove 201 is taken as the first rotary valve 101, the rotary valve at the second containing groove 202 is taken as the second rotary valve 102, the valve channel 112 of the first rotary valve 101 which is rotated to the first preset position enables the processing cavity 210 to be communicated with the amplification cavity 220, the valve channel 112 of the second rotary valve 102 which is rotated to the second preset position enables the amplification cavity 220 to be communicated with the air outlet 230, and the first rotary valve 101 which is rotated away from the first preset position and the second rotary valve 102 which is rotated away from the second preset position enable the amplification cavity 220 to be kept sealed.

Specifically, the chip main body 200 is in the form of a strip plate, and is made of a hard plastic material such as PP, PC, etc., a processing chamber 210 and an amplification chamber 220 are disposed therein, and a first receiving groove 201, a second receiving groove 202, and an air outlet 230 are sequentially disposed on a side surface with a larger area, where the first receiving groove 201 is located between the processing chamber 210 and the amplification chamber 220, and the second receiving groove 202 is located between the air outlet 230 and the amplification chamber 220. In addition, a plurality of microchannels are further disposed in the chip body 200, and the processing chamber 210, the first receiving chamber 201, the amplification chamber 220, the second receiving chamber 202 and the gas outlet 230 are sequentially communicated with each other through the microchannels.

A rotary valve is disposed in each of the first receiving chamber 201 and the second receiving chamber 202, and for the convenience of distinguishing, the rotary valve disposed in the first receiving chamber 201 is named as a first rotary valve 101, and the rotary valve disposed in the second receiving chamber 202 is named as a second rotary valve 102. The sealing element 110 in the first rotary valve 101 is completely accommodated in the first accommodating groove 201, and the torque is applied to the supporting element 120 in the first rotary valve 101 to drive the sealing element 110 in the first accommodating groove 201 to rotate synchronously, and during the rotation process, the position of the opening of the valve passage 112 disposed on the sealing element 110 is changed, so that the position of the first rotary valve 101 when the processing chamber 210 and the amplification chamber 220 can be communicated through the valve passage 112 is set as a first preset position.

Correspondingly, the sealing element 110 in the second rotary valve 102 is also completely accommodated in the second accommodating groove 202, and the torque is applied to the supporting element 120 in the second rotary valve 102, so as to drive the sealing element 110 in the second accommodating groove 202 to rotate synchronously, and during the rotation process, the position of the opening of the valve channel 112 disposed on the sealing element 110 is changed, and the position of the second rotary valve 102 when the air outlet 230 and the amplification chamber 220 are communicated through the valve channel 112 is set as a second preset position.

Be provided with on the inner wall of amplification chamber 220 and gather hole 221, be provided with microcentrifuge tube (EP pipe 300) in the below of gathering hole 221, when the liquid that awaits measuring in the processing chamber 210 flows to gathering hole 221 department, the liquid that awaits measuring can flow into in the EP pipe 300.

In a specific using process, firstly, a liquid to be detected, which needs to be detected, is input into the processing chamber 210, then the first rotary valve 101 is rotated to a first preset position, the second rotary valve 102 is rotated to a second preset position, and at this time, the processing chamber 210, the amplification chamber 220 and the air outlet 230 are communicated through the valve channel 112 of the first rotary valve 101 and the valve channel 112 of the second rotary valve 102. Then, the liquid to be measured in the processing chamber 210 flows to the amplification chamber 220 through the valve channel 112 of the first rotary valve 101 under the action of its own gravity or the action of the negative pressure air valve disposed at the air outlet 230, and in the process, the air in the amplification chamber 220 is pushed out from the air outlet 230. The liquid to be detected flowing into the amplification chamber 220 falls into the EP tube 300 when flowing to the collection hole 221. After a sufficient amount of the liquid to be measured is contained in the EP tube 300, the first rotary valve 101 and the second rotary valve 102 are respectively rotated, so that the amplification chamber 220 is no longer communicated with the gas outlet 230 and the processing chamber 210, thereby sealing the amplification chamber 220.

It can be understood that, by adopting the rotary valve, the sealing performance of the amplification chamber 220 in the detection process is effectively optimized, and the structure of the PCR chip is simplified.

In some embodiments of the present invention, there are three rotary valves, the chip main body 200 further has a first air passage 250, a second air passage 260 and a plurality of independent material loading chambers 240, the chip main body 200 further has a third receiving groove 203, the third receiving groove 203 is located between the material loading chambers 240 and the processing chamber 210, the third receiving groove 203 is communicated with the processing chamber 210 and each material loading chamber 240, one rotary valve is disposed at the third receiving groove 203, the rotary valve at the third receiving groove 203 is taken as the third rotary valve 103, the valve passage 112 of the third rotary valve 103 rotated to the third predetermined position enables the material charging chamber 240 to be communicated with the processing chamber 210, the first air passage 250 is communicated with the processing chamber 210 and the first receiving groove 201, the second air passage 260 is communicated with the first receiving groove 201 and the air outlet 230, and the valve passage 112 of the first rotary valve 101 rotated to the fourth predetermined position enables the first air passage 250 to be communicated with the second air passage 260.

Specifically, a plurality of feeding cavities 240 are further disposed in the chip main body 200, and the feeding cavities 240 are independent from each other, that is, the feeding cavities 240 are not directly communicated with each other. The buffer solution, the magnetic bead solution, the ethanol solution, the nucleic acid eluent, or the nucleic acid amplification premix, etc. are disposed in each feeding chamber 240. At this time, the loading chamber 240, the processing chamber 210, and the amplification chamber 220 are disposed in sequence. The chip main body 200 is further provided with a third containing groove 203 between each charging cavity 240 and the processing cavity 210, and the third containing groove 203 is respectively communicated with each charging cavity 240 and the processing cavity 210 through micro-pipes.

Meanwhile, the chip main body 200 is further provided with liquid inlets 241 corresponding to the feeding cavities 240 one by one, and corresponding liquid in the feeding cavities 240 is input through the liquid inlets 241.

The PCR chip is further provided with a third rotary valve, and the third rotary valve is installed in the third containing groove 203. For the convenience of distinguishing, the rotary valve in the third containing groove 203 is named as a third rotary valve 103. At this time, the sealing element 110 of the third rotary valve 103 is completely accommodated in the third accommodating groove 203, and the torque is applied to the supporting element 120 of the third rotary valve 103, so as to drive the sealing element 110 in the third accommodating groove 203 to rotate synchronously, and during the rotation process, the position of the opening of the valve channel 112 disposed on the sealing element 110 is changed, so that the position of the third rotary valve 103 when the feeding chamber 240 and the amplification chamber 220 can communicate through the valve channel 112 is set as a third preset position. It will be appreciated that the third predetermined position is generally indicated as a plurality of independent feed chambers 240 are provided, and only one feed chamber 240 is provided which communicates with the process chamber 210 via the valve passage 112 at a time, i.e. the third predetermined position comprises a number of sub-positions equal to the number of feed chambers 240 during operation.

In addition, a first air channel 250 and a second air channel 260 are disposed in the chip body 200, and the first air channel 250 and the second air channel 260 function to exhaust the air in the processing chamber 210 directly from the air outlet 230 by bypassing the amplification chamber 220. The first air passage 250 communicates the processing chamber 210 with the first receiving groove 201, and the second air passage 260 communicates the first receiving groove 201 with the air outlet 230. At this time, the position of the first rotary valve 101 when the valve passage 112 in the first rotary valve 101 communicates the first air passage 250 with the second air passage 260 is set to the fourth preset position. It will be appreciated that the first rotary valve 101 at the fourth predetermined position will block the communication between the treatment chamber 210 and the amplification chamber 220, and the first rotary valve 101 at the first predetermined position will also block the communication between the first air passage 250 and the second air passage 260.

In a specific use process, firstly, a corresponding liquid is input into each feeding cavity 240 through the liquid inlet 241, then the feeding cavity 240 where the liquid which needs to be fed into the processing cavity 210 in advance is located is selected, and then the third rotary valve 103 is rotated to a sub-position corresponding to the feeding cavity 240 in the third preset position, so that the feeding cavity 240 is communicated with the processing cavity 210. At this time, the first rotary valve 101 is rotated to the fourth preset position, so that the first air passage 250 is communicated with the second air passage 260, and then the liquid flows into the processing chamber 210 under the self-gravity of the liquid in the charging chamber 240 or the negative pressure air valve provided at the air outlet 230. In the process, the air in the processing chamber 210 passes through the first air passage 250, the valve passage 112 of the first rotary valve 101 and the second air passage 260 and is discharged from the air outlet 230.

Similarly, when it is necessary to add the liquid in the other feeding chamber 240 to the processing chamber 210, it is only necessary to rotate the third rotary valve 103 to the sub-position corresponding to the third preset position. The liquid in the processing chamber 210 becomes the liquid to be measured after being pre-processed by mixing or heating.

Finally, the first rotary valve 101 is rotated to the first preset position, and the liquid to be tested in the processing chamber 210 is transferred to the amplification chamber 220 and finally falls into the EP tube 300 as described above.

It can be understood that, by such a design, a plurality of different types of liquids can be added at a time, and these liquids can be mixed in the processing chamber 210 by themselves, and finally, the liquids are combined into the liquid to be detected, and then enter the amplification chamber 220, and are collected by the corresponding EP tube 300, so that the detection efficiency is effectively improved.

In some embodiments of the present invention, a negative pressure air pump is further included, and the negative pressure air pump is disposed at the air outlet 230.

Specifically, a negative pressure air pump is provided at the air outlet 230. The negative pressure air pump is used for pumping air out of the processing chamber 210 and/or the amplification chamber 220 to form negative pressure in the processing chamber 210 and/or the amplification chamber 220, so that the flowing speed of the liquid in the processing chamber 210 and/or the amplification chamber 220 is increased, and the detection efficiency is improved.

In some embodiments of the present invention, a buffer cavity 270 is further disposed in the chip main body 200, and the buffer cavity 270 is located between the air outlet 230 and the second receiving groove 202 and is communicated with the air outlet 230 and the second receiving groove 202.

Specifically, the chip main body 200 is further provided with a buffer cavity 270 between the air outlet 230 and the second receiving groove 202, and the buffer cavity 270 is respectively communicated with the air outlet 230 and the second receiving groove 202 through the micro channel. In practical use, when the second rotary valve 102 is located at the second preset position, the negative pressure air pump will pump out air in the amplification chamber 220, so that a negative pressure is formed in the amplification chamber 220, and thus the liquid to be measured is driven to flow in the amplification chamber 220.

It can be understood that, in this process, it is inevitable that a part of the liquid to be tested flows out from the amplification chamber 220 through the valve channel 112 in the second rotary valve 102 and flows towards the gas outlet 230, and the buffer chamber 270 is configured to enable the part of the liquid to be tested to be effectively buffered, so as to prevent the liquid to be tested from flowing into the gas outlet 230 and causing the gas outlet 230 to be blocked or flowing outside the chip main body 200.

In some embodiments of the present invention, the chip main body 200 further includes a positive pressure air pump, the air blowing port is connected to the second air channel 260, and the positive pressure air pump is disposed at the air blowing port.

Specifically, the chip main body 200 is further provided with an air blowing port communicated with the second air channel 260, and the air blowing port is provided with a positive pressure air pump. After a sufficient amount of liquid to be detected has been collected in the EP tube 300, the sealing performance of the amplification chamber 220 does not need to be maintained continuously, at this time, the first rotary valve 101 is rotated to the fourth preset position, so that the first air passage 250 is communicated with the second air passage 260, at this time, the positive pressure air pump blows air towards the second air passage 260 through the air blowing port, the air flow is finally blown into the treatment chamber 210, and then the residual liquid in the treatment chamber 210 is blown back into the feeding chamber 240 communicated with the valve channel 112 in the third rotary valve 103 rotated to the third preset position at this time.

It will be appreciated that such a design eliminates the need for cleaning the process chamber 210 at a later time, reduces cleaning steps, and improves cleaning efficiency.

As an improvement of the above scheme, the positive pressure air pump and the negative pressure air pump are the same air pump, and can respectively perform the functions of air suction and air blowing at different times, and at the moment, the air outlet 230 and the air blowing opening are the same opening. It will be appreciated that such a design simplifies the structure of the PCR chip.

In some embodiments of the present invention, a plurality of collection holes 221 are further disposed in the chip body 200 and are in communication with the amplification chamber 220, and the collection holes 221 are independent of each other.

Specifically, a plurality of collecting holes 221 are disposed on the inner wall of the amplification chamber 220, and the collecting holes 221 are independent from each other and are respectively communicated with the first receiving groove 201 and the second receiving groove 202 through microchannels which are not directly communicated with each other. It will be appreciated that the first predetermined position also includes a plurality of sub-positions, and that the valve passage 112 of the first rotary valve 101 at different sub-positions is connected to different ones of the pick-up holes 221. Depending on the liquids added to the processing chamber 210, the mixed liquids may be sequentially introduced into the amplification chamber 220 through the valve passages 112 of the first rotary valve 101 at the corresponding sub-positions, and introduced into the desired EP tube 300 through the desired collection holes 221. The design enables the PCR chip to carry out 'single-sample multi-index' detection.

In some embodiments of the invention, the aperture of the collection well 221 decreases in a direction away from the amplification chamber 220.

Specifically, the collection hole 221 is in the shape of a circular truncated cone, and the diameter of the collection hole gradually decreases toward the direction away from the amplification chamber 220. It can be understood that, with such a design, the liquid flowing into the EP tube 300 through the collecting hole 221 is guided by the sidewall of the collecting hole 221 with a certain inclination angle during the flowing process, and the large caliber of the contact part of the collecting hole 221 and the amplification cavity 220 also accelerates the flowing speed of the liquid to be detected, thereby improving the detection efficiency.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention.