阅读说明：本技术 一种提高机体免疫力的猪饲料及其制备方法 (Pig feed for improving immunity of organism and preparation method thereof ) 是由 王大文 徐林香 贾友松 戚玉环 于 2021-09-02 设计创作，主要内容包括：本发明公开了一种提高机体免疫力的猪饲料及其制备方法。所制备的猪饲料为猪喜爱的酸甜口味,并具有水果、甘草、谷实综合香味。促采食、口感好、易消化、易吸收,并有助于促进生长,保持肠道健康,降低粪便臭味,有效提高猪的免疫力和抗病能力。(The invention discloses a pig feed for improving immunity of an organism and a preparation method thereof. The prepared pig feed has a sour and sweet taste which is loved by pigs, and has comprehensive fragrance of fruits, liquorice and grains. The pig feed has the advantages of promoting ingestion, good taste, easy digestion and absorption, promoting growth, keeping intestinal health, reducing feces odor, and effectively improving immunity and disease resistance of pigs.)

1. The preparation method of the pig feed for improving the immunity of the organism is characterized by comprising the following steps:

1) mixing 84-130 parts of active ingredients, 20-35 parts of grass meal, 0.2-0.3 part of multivitamin, 0.4-0.7 part of garlicin and 10-20 parts of water uniformly by weight to obtain a premix;

2) and (3) putting the premix into a granulator for granulation to obtain the pig feed for improving the immunity of the organism.

2. The method for preparing the swine feed for improving immunity according to claim 1, wherein the premix further comprises 6 to 14 parts of polysaccharide additive.

3. The method of claim 2, wherein the polysaccharide additive is brown algae polysaccharide and/or nicandra physaloides seed polysaccharide.

4. The method for preparing the pig feed for improving the immunity of the organism according to claim 3, wherein the method for preparing the nicandra physaloides seed polysaccharide comprises the following steps:

putting the nicandra physaloides seeds into water at the temperature of 30-50 ℃, stirring for 10-20min at the speed of 100-300rpm under the assistance of 400-800W ultrasonic waves at the frequency of 25-30kHz, wherein the material-liquid ratio is 1 g: (20-50) mL, separating a liquid phase from a solid phase by using a separator after stirring, taking the liquid phase, and freeze-drying to obtain physalis pseudo-acid seed polysaccharide;

the preparation method of the brown algae polysaccharide comprises the following steps:

washing brown algae with water, drying the brown algae in an oven at 40-55 ℃ for 2-4 days, taking out the brown algae, crushing the brown algae, and sieving the crushed brown algae with a 20-40-mesh sieve to obtain brown algae powder, wherein the ratio of the brown algae powder to the water is 1 g: (30-60) mL, mixing and soaking for 0.5-1h, then heating and extracting at 100 ℃ for 1-4h, naturally cooling and then carrying out suction filtration to obtain brown algae polysaccharide crude extract, carrying out rotary evaporation concentration on the brown algae polysaccharide crude extract at 60-80 ℃ to 1/3-1/2 of the original volume to obtain concentrated solution, adding absolute ethyl alcohol of which the volume is 3-5 times that of the concentrated solution, standing and precipitating at 2-4 ℃ for 0.5-2d, centrifuging at 2000-4000rpm for 5-10min to remove supernatant to obtain alcohol precipitated solid, and placing the alcohol precipitated solid in an oven at 40-55 ℃ for drying for 5-8h to obtain the brown algae polysaccharide.

5. The method for preparing the pig feed for improving the immunity of the organism according to claim 1, wherein the preparation method of the active ingredients comprises the following steps:

(1) uniformly mixing 30-50 parts of corn flour, 20-45 parts of sorghum flour, 15-30 parts of bran and 8-20 parts of bran coat according to parts by weight to obtain a coarse mixture;

(2) mixing bean dregs, bacillus subtilis, the crude mixture and 1-3 wt% of sodium chloride aqueous solution according to the material-liquid ratio (10-20) g: (2-5) g: (80-100) g: (50-80) mL to obtain a premix, putting the premix into a fermentation tank, and carrying out aerobic fermentation for 24-48h at 35-37 ℃ to obtain a fermentation main material;

(3) the mass ratio of the fermentation main material to the premix is (4-10): (80-120) and uniformly mixing the small peptide chelate salt to obtain the active ingredient.

6. The method for preparing the pig feed for improving the immunity of the organism according to claim 1, wherein the preparation method of the active ingredients comprises the following steps:

(1) uniformly mixing 30-50 parts of corn flour, 20-45 parts of sorghum flour, 15-30 parts of bran and 8-20 parts of bran coat according to parts by weight to obtain a coarse mixture;

(2) mixing bean dregs, bacillus subtilis, the crude mixture and 1-3 wt% of sodium chloride aqueous solution according to the material-liquid ratio (10-20) g: (2-5) g: (80-100) g: (50-80) mL to obtain a premix, and adding the premix in a mass ratio of (4-10): (80-120), mixing uniformly, placing in a fermentation tank, and carrying out aerobic fermentation at 35-37 ℃ for 24-48h to obtain the active ingredient.

7. The method for preparing the pig feed for improving the immunity of the organism according to claim 5 or 6, wherein the method for preparing the small peptide chelate salt comprises the following steps:

s1, cleaning shrimp shells, airing water, crushing, and screening by a 40-100-mesh sieve to obtain shrimp shell powder;

s2, placing the shrimp shell powder in water at the temperature of 50-70 ℃, adjusting the pH value to 8-9.5 by using NaOH aqueous solution, and adding alkaline protease for enzymolysis, wherein the feed-liquid ratio of the shrimp shell powder to the alkaline protease to the water is (30-50) g: (4-8) g: (100) 120 mL, performing enzymolysis for 6-10h, naturally cooling to room temperature, performing ultrasonic treatment at 200-;

s3, heating the shrimp shell small peptide liquid to 60-70 ℃ at a heating rate of 3-5 ℃/min, and then adding sulfate, wherein the ratio of the sulfate to the shrimp shell small peptide liquid is 1 g: (5-12) mL, then regulating the pH value to 6-7 with a hydrochloric acid solution, stirring at 600rpm of 300-;

and S4, carrying out freeze drying on the small peptide chelate salt solution to obtain the small peptide chelate salt.

8. The method of claim 7, wherein the sulfate is a mixture of ferrous sulfate, copper sulfate, manganese sulfate and zinc sulfate.

9. The method for preparing the pig feed for improving the immunity of the organism according to claim 1, wherein the method for preparing the green grass powder comprises the following steps: mixing Chinese cabbage, herba Medicaginis, herba Cichorii stem and leaf, and herba Amaranthi Tricoloris uniformly, pulverizing, and sieving with 20-40 mesh sieve to obtain green grass powder.

10. A pig feed for enhancing immunity, prepared by the method of any one of claims 1 to 9.

Technical Field

The invention belongs to the technical field of feeds, and particularly relates to a preparation method of a pig feed for improving immunity of an organism.

Background

The pork, one of the main poultry, contains rich protein, fat, carbohydrate, calcium, iron, phosphorus and other nutrients. Pork is one of important animal food on a dining table, and has a particularly delicious meat taste after cooking because fibers are thin and soft, connective tissues are few, and muscle tissues contain much intramuscular fat. At present, pork accounts for about 67 percent of the meat consumption structure of residents in China, and is a main meat product consumed by people. With the improvement of the living standard of people, the pursuit of people for the life with healthy quality is increasing day by day, and pork which is healthy, good in taste and high in nutrition is favored.

At present, the pig farmers in large scale in China feed with the existing pig feed formula, in order to improve the growth speed of pork and the feed reward and improve the immunocompetence of pigs, most pig feeds contain more antibiotics and chemically synthesized medicines, which not only harm the health of pigs, but also cause the hidden danger of food safety, and then harm the health of human beings. The Chinese invention patent CN 108522819A discloses a compound pig feed for improving immunity and promoting growth and a preparation method thereof, wherein the compound feed comprises the following components in parts by weight: 30-55 parts of corn flour, 30-55 parts of potato flour, 20-45 parts of sorghum flour, 5-20 parts of bran, 2-12 parts of bran coat, 1-5 parts of high-quality fish meal, 20-40 parts of green grass powder, 1-5 parts of compound vitamin and 5-20 parts of Chinese herbal medicine additive powder; the invention also provides a preparation method of the compound pig feed. The feed disclosed by the invention is reasonable in combination, can improve the digestion and absorption capacity and promote growth, and the pigs can comprehensively absorb nutrition and promote growth during growth, so that the immunity of the pigs can be effectively improved.

However, the digestion and absorption of pigs are easily affected by a large amount of crude fiber mixture, the appetite of pigs can be weakened due to Chinese herbal medicine components such as houttuynia cordata and the like, and the pigs are easily affected by diseases and even die due to lack of nutrition particularly in seasonal changes, so that huge economic loss is caused.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides a preparation method of a pig feed for improving the immunity of the organism, which comprises the following steps:

1) mixing 84-130 parts of active ingredients, 20-35 parts of grass meal, 0.2-0.3 part of multivitamin, 0.4-0.7 part of garlicin and 10-20 parts of water uniformly by weight to obtain a premix;

2) and (3) putting the premix into a granulator for granulation to obtain the pig feed for improving the immunity of the organism.

The preparation method of the active ingredients comprises the following steps:

(1) uniformly mixing 30-50 parts of corn flour, 20-45 parts of sorghum flour, 15-30 parts of bran and 8-20 parts of bran coat according to parts by weight to obtain a coarse mixture;

(2) mixing bean dregs, bacillus subtilis, the crude mixture and 1-3 wt% of sodium chloride aqueous solution according to the material-liquid ratio (10-20) g: (2-5) g: (80-100) g: (50-80) mL to obtain a premix, putting the premix into a fermentation tank, and carrying out aerobic fermentation at 35-37 ℃ for 24-48h to obtain a fermentation main material.

(3) The mass ratio of the fermentation main material to the premix is (4-10): (80-120) and uniformly mixing the small peptide chelate salt to obtain the active ingredient.

After the bacillus subtilis is fermented, enzymes such as beta-mannase, alpha-amylase, protease, peptidase, lipase, cellulase and the like can be generated, so that cellulose, hemicellulose and the like in corn flour, sorghum flour, bran and bran coat are decomposed into oligosaccharide and monosaccharide, the feed is more palatable, the absorption and digestion of pigs are facilitated, the absorption and utilization of nutrients by the pigs are promoted, the conversion rate of the pigs to the feed is improved, and the phenomenon that the pigs cannot eat meat is avoided.

The fermentation of the bean dregs can improve the taste, provide rich amino acid, eliminate harmful substances in the bean dregs, such as gossypol, aspergillus flavus, staphylococcus and other toxins, and avoid diarrhea caused by the pollution of animal intestinal tracts. The bean dregs are fermented to generate a large amount of probiotics to form a beneficial flora, in the intestinal tract, the bacillus subtilis causes intestinal hypoxia to promote the growth of beneficial anaerobes and indirectly inhibit the growth of other pathogenic bacteria, so that the microbial balance in the intestinal tract of the pig is synergistically maintained, the immunity and disease resistance of the pig are improved, and the pig is rarely ill or cannot be ill.

The preparation method of the small peptide chelate salt comprises the following steps:

s1, cleaning shrimp shells, airing water, crushing, and screening by a 40-100-mesh sieve to obtain shrimp shell powder;

s2, placing the shrimp shell powder in water at the temperature of 50-70 ℃, adjusting the pH to 8-9.5 by using 1-2M NaOH aqueous solution, adding alkaline protease for enzymolysis, wherein the feed-liquid ratio of the shrimp shell powder to the alkaline protease to the water is (30-50) g: (4-8) g: (100) 120 mL, performing enzymolysis for 6-10h, naturally cooling to room temperature, performing ultrasonic treatment at 200-;

s3, heating the shrimp shell small peptide liquid to 60-70 ℃ at a heating rate of 3-5 ℃/min, and then adding sulfate, wherein the ratio of the sulfate to the shrimp shell small peptide liquid is 1 g: (5-12) mL, then regulating the pH value to 6-7 by using a 1-2M hydrochloric acid solution, stirring at 600rpm of 300-;

and S4, carrying out freeze drying on the small peptide chelate salt solution to obtain the small peptide chelate salt.

The sulfate is a mixture of ferrous sulfate, copper sulfate, manganese sulfate and zinc sulfate.

Under the action of alkaline protease, the three-dimensional helical structure of collagen in the shrimp shells is opened and then cut into different fragments to form small peptides with small molecular weight. After eating, the small peptide can not be hydrolyzed by pepsin, pancreatin, amylase and digestive enzyme for the second time, can directly enter the small intestine in an intact form to be absorbed, enters blood circulation, directly participates in the synthesis of tissue protein, and plays a role in physiological regulation. Metal ions such as iron, manganese, zinc, copper and the like are indispensable microelements in the pig body and are very important for regulating the immune function. However, inorganic salt added separately in the feed is easy to precipitate with components such as phytic acid and the like, and is difficult to digest and absorb, thereby causing negative effects.

The small peptide chelate salt is a novel structure in which metal ions are embedded between two small peptide molecules, the small peptide molecules clamp the metal ions like crab pincers to form a stable chelate structure, and then the metal ions are conveyed into blood through a small peptide channel, so that the metal ions and the small peptide are absorbed by an organism together, and the absorption rate is effectively improved. The shrimp shell also contains abundant chitin and calcium carbonate, and can regulate immunity and supplement calcium.

The preparation method of the green grass powder comprises the following steps: mixing Chinese cabbage, herba Medicaginis, herba Cichorii stem and leaf, and herba Amaranthi Tricoloris uniformly, pulverizing, and sieving with 20-40 mesh sieve to obtain green grass powder.

The addition of the green grass powder can reduce the cost of the feed, and meanwhile, the green grass powder is fresh, tender and juicy, is favorable for the taste of pigs and can promote the ingestion. Chicory can also strengthen stomach and promote digestion, and the Amaranthus viridis has the effects of killing insect poison and facilitating urination and defecation.

Preferably, the premix also comprises 6-14 parts of polysaccharide additive.

The polysaccharide additive is brown algae polysaccharide and/or nicandra physaloides seed polysaccharide.

Preferably, the polysaccharide additive is prepared from brown algae polysaccharide and nicandra physaloides seed polysaccharide in a mass ratio of 1: (1.5-2.2).

The preparation method of the nicandra physaloides seed polysaccharide comprises the following steps:

putting the nicandra physaloides seeds into water at the temperature of 30-50 ℃, stirring for 10-20min at the speed of 100-300rpm under the assistance of 400-800W ultrasonic waves at the frequency of 25-30kHz, wherein the material-liquid ratio is 1 g: (20-50) mL, separating the liquid phase and the solid phase by using a separator after stirring, taking the liquid phase, and freeze-drying to obtain the physalis pseudo-acid seed polysaccharide.

The preparation method of the brown algae polysaccharide comprises the following steps:

washing brown algae with water, drying the brown algae in an oven at 40-55 ℃ for 2-4 days, taking out the brown algae, crushing the brown algae, and sieving the crushed brown algae with a 20-40-mesh sieve to obtain brown algae powder, wherein the ratio of the brown algae powder to the water is 1 g: (30-60) mL, mixing and soaking for 0.5-1h, then heating and extracting at 100 ℃ for 1-4h, naturally cooling and then carrying out suction filtration to obtain brown algae polysaccharide crude extract, carrying out rotary evaporation concentration on the brown algae polysaccharide crude extract at 60-80 ℃ to 1/3-1/2 of the original volume to obtain concentrated solution, adding absolute ethyl alcohol of which the volume is 3-5 times that of the concentrated solution, standing and precipitating at 2-4 ℃ for 0.5-2d, centrifuging at 2000-4000rpm for 5-10min to remove supernatant to obtain alcohol precipitated solid, and placing the alcohol precipitated solid in an oven at 40-55 ℃ for drying for 5-8h to obtain the brown algae polysaccharide.

The polysaccharide extract of Nicandra physaloides seed has effects of clearing away heat and toxic materials. In the mixing process, the nicandra physaloides seed polysaccharide is combined with metal ions such as iron, manganese, zinc, copper and the like in calcium and small peptide chelate salt in the shrimp shell powder to form gel, so that the gel has a coating effect on active ingredients such as fermentation main materials, green grass powder, vitamins and allicin, effectively thickens, keeps fresh, preserves the activity of the active ingredients, and prolongs the storage life of the feed. In addition, because of strong water absorption, the pigs have an increased satiety after eating the feed, which is helpful for avoiding dyspepsia caused by excessive eating.

The main components of fucoidan are algin, fucoidan, and brown algae starch. The pig feed has the effects of promoting cell growth, improving immunity and the like, absorbs water to generate gel, has good water retention, and delays the reduction of the content of active effective ingredients caused by excessive water loss in the storage process of the pig feed.

Further preferably, the preparation method of the pig feed for improving the immunity of the organism comprises the following steps:

1) according to the weight parts, 84-130 parts of active ingredients, 20-35 parts of grass meal, 6-14 parts of polysaccharide additives, 0.2-0.3 part of compound vitamins, 0.4-0.7 part of allicin and 10-20 parts of water are uniformly mixed and stirred to obtain premix;

2) and (3) putting the premix into a granulator for granulation to obtain the pig feed for improving the immunity of the organism.

The preparation method of the active ingredients comprises the following steps:

(1) uniformly mixing 30-50 parts of corn flour, 20-45 parts of sorghum flour, 15-30 parts of bran and 8-20 parts of bran coat according to parts by weight to obtain a coarse mixture;

(2) mixing bean dregs, bacillus subtilis, the crude mixture and 1-3 wt% of sodium chloride aqueous solution according to the material-liquid ratio (10-20) g: (2-5) g: (80-100) g: (50-80) mL to obtain a premix, and adding the premix in a mass ratio of (4-10): (80-120), mixing uniformly, placing in a fermentation tank, and carrying out aerobic fermentation at 35-37 ℃ for 24-48h to obtain the active ingredient.

The preparation method of the small peptide chelate salt comprises the following steps:

s1, cleaning shrimp shells, airing water, crushing, and screening by a 40-100-mesh sieve to obtain shrimp shell powder;

s2, placing the shrimp shell powder in water at the temperature of 50-70 ℃, adjusting the pH to 8-9.5 by using 1-2M NaOH aqueous solution, adding alkaline protease for enzymolysis, wherein the feed-liquid ratio of the shrimp shell powder to the alkaline protease to the water is (30-50) g: (4-8) g: (100) 120 mL, performing enzymolysis for 6-10h, naturally cooling to room temperature, performing ultrasonic treatment at 200-;

s3, heating the shrimp shell small peptide liquid to 60-70 ℃ at a heating rate of 5 ℃/min, and then adding sulfate, wherein the ratio of the sulfate to the shrimp shell small peptide liquid is 1 g: (5-12) mL, then adjusting the pH value to 6.5 by using a 1M hydrochloric acid solution, stirring at 600rpm of 300-;

and S4, carrying out freeze drying on the small peptide chelate salt solution to obtain the small peptide chelate salt.

The sulfate is a mixture of ferrous sulfate, copper sulfate, manganese sulfate and zinc sulfate; preferably, the sulfate is ferrous sulfate, copper sulfate, manganese sulfate and zinc sulfate according to a mass ratio of 1: (1-2): (2-3): (2-3).

The polysaccharide additive is brown algae polysaccharide and/or nicandra physaloides seed polysaccharide.

Preferably, the polysaccharide additive is prepared from brown algae polysaccharide and nicandra physaloides seed polysaccharide in a mass ratio of 1: (1.5-2.2).

The preparation method of the nicandra physaloides seed polysaccharide comprises the following steps:

putting the nicandra physaloides seeds into water at the temperature of 30-50 ℃, stirring for 10-20min at the speed of 100-300rpm under the assistance of 400-800W ultrasonic waves at the frequency of 25-30kHz, wherein the material-liquid ratio is 1 g: (20-50) mL, separating the liquid phase and the solid phase by using a separator after stirring, taking the liquid phase, and freeze-drying to obtain the physalis pseudo-acid seed polysaccharide.

The preparation method of the brown algae polysaccharide comprises the following steps:

washing brown algae with water, drying the brown algae in an oven at 40-55 ℃ for 2-4 days, taking out the brown algae, crushing the brown algae, and sieving the crushed brown algae with a 20-40-mesh sieve to obtain brown algae powder, wherein the ratio of the brown algae powder to the water is 1 g: (30-60) mL, mixing and soaking for 0.5-1h, then heating and extracting at 100 ℃ for 1-4h, naturally cooling and then carrying out suction filtration to obtain brown algae polysaccharide crude extract, carrying out rotary evaporation concentration on the brown algae polysaccharide crude extract at 60-80 ℃ to 1/3-1/2 of the original volume to obtain concentrated solution, adding absolute ethyl alcohol of which the volume is 3-5 times that of the concentrated solution, standing and precipitating at 2-4 ℃ for 0.5-2d, centrifuging at 2000-4000rpm for 5-10min to remove supernatant to obtain alcohol precipitated solid, and placing the alcohol precipitated solid in an oven at 40-55 ℃ for drying for 5-8h to obtain the brown algae polysaccharide.

The preparation method of the green grass powder comprises the following steps: mixing Chinese cabbage, herba Medicaginis, herba Cichorii stem and leaf, and herba Amaranthi Tricoloris uniformly, pulverizing, and sieving with 20-40 mesh sieve to obtain green grass powder. The Chinese cabbage, the alfalfa, the chicory stem and leaf and the amaranthus viridis are mixed according to the mass ratio of 1: (1-2): (1-2): (1-2).

In the fermentation process, when small peptide chelate salt exists, a nitrogen source required for enzyme production is added to the bacillus subtilis, and metal ions in the nitrogen source can effectively promote the enzyme production rate, so that the enzymolysis speed of crude fibers is increased. Particularly, the manganese ions can remarkably promote the production rate of the beta-mannase, the beta-mannase can eliminate the interference of mannan in the feed on glucose absorption, and the energy digestibility of the corn bean dregs and the like is greatly improved. The beta-mannase is a multifunctional growth promoter, and can promote the secretion of insulin-like growth factors, the synthesis of proteins, the lean meat percentage and the growth.

At this time, part of the small peptides in the small peptide chelate salt are decomposed into amino acids, and then converted into amino acid chelate salts. Under normal conditions, the absorption speed and the protein synthesis speed of the small peptide are faster than those of amino acid, and the biological value is higher. When pigs eat feed, the bacillus subtilis quickly consumes free oxygen in intestinal tracts to cause intestinal tract hypoxia, so that the growth of anaerobic probiotics is facilitated on one hand, and the absorption of small peptides is inhibited on the other hand, because the absorption of the small peptides mainly depends on H⁺Or Ca²⁺The ion concentration conductance is a transport process that requires energy consumption. The absorption of free amino acids is an active dependence on Na⁺The active transfer process of the pump is not influenced in a low oxygen environment, so that the biological value is higher when the small peptide and the amino acid exist simultaneously in the formula。

Has the advantages that: the pig feed for improving the immunity of the organism, which is prepared by the invention, has a sour and sweet taste which is loved by pigs, and has comprehensive fragrance of fruits, liquorice and grains. The pig feed has the advantages of promoting ingestion, good taste, easy digestion and absorption, promoting growth, keeping intestinal health, reducing feces odor, and effectively improving immunity and disease resistance of pigs.

Detailed Description

The raw materials used in the examples were as follows:

corn flour, purchased from sierra, bioengineering, ltd, cat #: REL 0941.

Sorghum flour, purchased from mingwang food ltd.

Bran, purchased from kyushu chemical limited, shandong, cat # o: 781-23578.

Bran coat, purchased from Shijiazhuang Zhuhui agricultural science and technology Limited, goods number: 020208.

the bean dregs are purchased from information services Co., Ltd, constant in Foshan City, and have a product number of 001.

Bacillus subtilis, latin name: bacillus subtilis, accession number: ACCC 60429, purchased from China agricultural microbial culture Collection management center.

Shrimp shells, purchased from Haixgchang Hai fodder Co.

Multivitamins, product name: heqie multivitamin (piglet formula) is purchased from Heqie animal nutrition science and technology Limited company of Foshan city, and each kilogram of the Heqie multivitamin vitamin A contains 40000000IU, vitamin B68000 mg, vitamin D38500000 IU, vitamin B12100 mg, vitamin K5000 mg, folic acid 4000mg, vitamin E80000 mg, nicotinamide 10000mg, vitamin B18000 mg, calcium pantothenate 50000mg, vitamin B220000 mg and biotin 500 mg.

Alkaline protease, purchased from Anhui Mukokang food Co., Ltd, CAS No: 9014-01-1, enzyme activity: 10 ten thousand U/g.

Zinc sulfate, purchased from the south of china for chemical industry limited, CAS No.: 7733-02-0, cat # s: 06.

manganese sulfate, purchased from jinan connotation chemical limited, CAS No.: 10034-96-5. Copper sulfate, purchased from Shandong Wang Song Biotech Co., Ltd., CAS number: 7758-98-7

Ferrous sulfate, purchased from Zibo Co-Ying chemical technology Co., Ltd, CAS number: 7782-63-0.

Cabbage (Brassica pekinensis (Lour.) Rupr.) is commercially available as Wujin white, alfalfa (Lotuscoronitus L), chicory (Cichorium intybus L.) stem and leaf, and Amaranthus hypochondriacus (Amaranthus hypochondriatus L.).

Nicandra physaloides seed, commercially available.

Brown algae, variety hizikia fusiforme, purchased from the west ao north ao deep sea bab farm in wenzhou city, cargo number: 001.

allicin, purchased from knack chemical limited, goods number: 096.

example 1

A preparation method of pig feed for improving immunity of organisms comprises the following steps:

1) mixing and stirring 100 parts of active ingredients, 30 parts of grass meal, 0.3 part of compound vitamin, 0.6 part of allicin and 15 parts of water uniformly according to parts by weight to obtain a premix;

2) and (3) putting the premix into a granulator for granulation to obtain the feed. The pig feed for improving the immunity of the organism is cylindrical particles, the particle size of the cylindrical particles is 0.6cm, the length of the cylindrical particles is 1.5cm, and the water content of the cylindrical particles is 12%.

The preparation method of the active ingredients comprises the following steps:

(1) uniformly mixing 40 parts of corn flour, 30 parts of sorghum flour, 20 parts of bran and 16 parts of bran coat according to parts by weight to obtain a coarse mixture;

(2) mixing the bean dregs, the bacillus subtilis, the crude mixture and a 2 wt% sodium chloride aqueous solution according to a material-liquid ratio of 15 g: 3 g: 85 g: mixing 60mL of the mixture to obtain a premix, placing the premix in a fermentation tank, and carrying out aerobic fermentation for 36 hours at 37 ℃ to obtain a main fermentation material;

(3) adding the fermentation main material and the premix in a mass ratio of 8: 100 of small peptide chelate salt, and uniformly mixing to obtain the active component.

The preparation method of the small peptide chelate salt comprises the following steps:

s1, cleaning shrimp shells, airing water, crushing, and screening by a 60-mesh sieve to obtain shrimp shell powder;

s2, placing the shrimp shell powder in water at 60 ℃, adjusting the pH to 9 by using 1M NaOH aqueous solution, adding alkaline protease for enzymolysis, wherein the feed-liquid ratio of the shrimp shell powder to the alkaline protease to the water is 40 g: 6 g: 110mL, performing enzymolysis for 8 hours, naturally cooling to room temperature, performing ultrasonic treatment at 300W and 30kHz for 25min to inactivate enzyme, filtering with four layers of common gauze of 100 meshes, and taking filtrate to obtain shrimp shell small peptide liquid;

s3, heating the shrimp shell small peptide liquid to 65 ℃ at a heating rate of 5 ℃/min, and then adding sulfate, wherein the ratio of the sulfate to the shrimp shell small peptide liquid is 1 g: 8mL, then regulating the pH value to 6.5 by using a 1M hydrochloric acid solution, stirring at 400rpm for 6.5h for chelation reaction, naturally cooling to room temperature after the reaction is finished, and performing suction filtration by using a Buchner funnel to obtain a small peptide chelate salt solution;

and S4, carrying out freeze drying on the small peptide chelate salt solution to obtain the small peptide chelate salt.

The sulfate is ferrous sulfate, copper sulfate, manganese sulfate and zinc sulfate according to a mass ratio of 1: 1: 2: 2.

The preparation method of the green grass powder comprises the following steps: the method comprises the following steps of mixing Chinese cabbage, alfalfa, chicory stem leaves and amaranthus viridis according to the mass ratio of 1: 1: 1: 1, uniformly mixing, crushing, and sieving by a 40-mesh sieve to obtain the green grass powder.

Example 2

A preparation method of pig feed for improving immunity of organisms comprises the following steps:

1) mixing and stirring 100 parts of active ingredients, 30 parts of grass meal, 10 parts of polysaccharide additive, 0.3 part of vitamin complex, 0.6 part of garlicin and 15 parts of water uniformly according to parts by weight to obtain a premix;

2) and (3) putting the premix into a granulator for granulation to obtain the feed. The pig feed for improving the immunity of the organism is cylindrical particles, the particle size of the cylindrical particles is 0.6cm, the length of the cylindrical particles is 1.5cm, and the water content of the cylindrical particles is 12%.

The preparation method of the active ingredients comprises the following steps:

(1) uniformly mixing 40 parts of corn flour, 30 parts of sorghum flour, 20 parts of bran and 16 parts of bran coat according to parts by weight to obtain a coarse mixture;

(2) mixing the bean dregs, the bacillus subtilis, the crude mixture and a 2 wt% sodium chloride aqueous solution according to a material-liquid ratio of 15 g: 3 g: 85 g: mixing 60mL of the mixture to obtain a premix, placing the premix in a fermentation tank, and carrying out aerobic fermentation for 36 hours at 37 ℃ to obtain a main fermentation material;

(3) adding the fermentation main material and the premix in a mass ratio of 8: 100 of small peptide chelate salt, and uniformly mixing to obtain the active component.

The preparation method of the small peptide chelate salt comprises the following steps:

s1, cleaning shrimp shells, airing water, crushing, and screening by a 60-mesh sieve to obtain shrimp shell powder;

s2, placing the shrimp shell powder in water at 60 ℃, adjusting the pH to 9 by using 1M NaOH aqueous solution, adding alkaline protease for enzymolysis, wherein the feed-liquid ratio of the shrimp shell powder to the alkaline protease to the water is 40 g: 6 g: 110mL, performing enzymolysis for 8 hours, naturally cooling to room temperature, performing ultrasonic treatment at 300W and 30kHz for 25min to inactivate enzyme, filtering with four layers of common gauze of 100 meshes, and taking filtrate to obtain shrimp shell small peptide liquid;

s3, heating the shrimp shell small peptide liquid to 65 ℃ at a heating rate of 5 ℃/min, and then adding sulfate, wherein the ratio of the sulfate to the shrimp shell small peptide liquid is 1 g: 8mL, then regulating the pH value to 6.5 by using a 1M hydrochloric acid solution, stirring at 400rpm for 6.5h for chelation reaction, naturally cooling to room temperature after the reaction is finished, and performing suction filtration by using a Buchner funnel to obtain a small peptide chelate salt solution;

and S4, carrying out freeze drying on the small peptide chelate salt solution to obtain the small peptide chelate salt.

The sulfate is ferrous sulfate, copper sulfate, manganese sulfate and zinc sulfate according to a mass ratio of 1: 1: 2: 2.

The preparation method of the green grass powder comprises the following steps: the method comprises the following steps of mixing Chinese cabbage, alfalfa, chicory stem leaves and amaranthus viridis according to the mass ratio of 1: 1: 1: 1, uniformly mixing, crushing, and sieving by a 40-mesh sieve to obtain the green grass powder.

The polysaccharide additive is nicandra physaloides seed polysaccharide.

The preparation method of the nicandra physaloides seed polysaccharide comprises the following steps:

putting the nicandra physaloides seeds into water with the temperature of 40 ℃, stirring for 15min at 200rpm under the assistance of 500W and 25kHz ultrasonic waves, wherein the material-liquid ratio is 1 g: 35mL, separating the liquid phase and the solid phase by using a separator after stirring, taking the liquid phase, and freeze-drying to obtain the physalis pseudo-acid seed polysaccharide.

Example 3

A preparation method of pig feed for improving immunity of organisms comprises the following steps:

1) mixing and stirring 100 parts of active ingredients, 30 parts of grass meal, 10 parts of polysaccharide additive, 0.3 part of vitamin complex, 0.6 part of garlicin and 15 parts of water uniformly according to parts by weight to obtain a premix;

2) and (3) putting the premix into a granulator for granulation to obtain the feed. The pig feed for improving the immunity of the organism is cylindrical particles, the particle size of the cylindrical particles is 0.6cm, the length of the cylindrical particles is 1.5cm, and the water content of the cylindrical particles is 12%.

The preparation method of the active ingredients comprises the following steps:

(1) uniformly mixing 40 parts of corn flour, 30 parts of sorghum flour, 20 parts of bran and 16 parts of bran coat according to parts by weight to obtain a coarse mixture;

(2) mixing the bean dregs, the bacillus subtilis, the crude mixture and a 2 wt% sodium chloride aqueous solution according to a material-liquid ratio of 15 g: 3 g: 85 g: mixing 60mL of the mixture to obtain a premix, placing the premix in a fermentation tank, and carrying out aerobic fermentation for 36 hours at 37 ℃ to obtain a main fermentation material;

(3) adding the fermentation main material and the premix in a mass ratio of 8: 100 of small peptide chelate salt, and uniformly mixing to obtain the active component.

The preparation method of the small peptide chelate salt comprises the following steps:

s1, cleaning shrimp shells, airing water, crushing, and screening by a 60-mesh sieve to obtain shrimp shell powder;

s2, placing the shrimp shell powder in water at 60 ℃, adjusting the pH to 9 by using 1M NaOH aqueous solution, adding alkaline protease for enzymolysis, wherein the feed-liquid ratio of the shrimp shell powder to the alkaline protease to the water is 40 g: 6 g: 110mL, performing enzymolysis for 8 hours, naturally cooling to room temperature, performing ultrasonic treatment at 300W and 30kHz for 25min to inactivate enzyme, filtering with four layers of common gauze of 100 meshes, and taking filtrate to obtain shrimp shell small peptide liquid;

s3, heating the shrimp shell small peptide liquid to 65 ℃ at a heating rate of 5 ℃/min, and then adding sulfate, wherein the ratio of the sulfate to the shrimp shell small peptide liquid is 1 g: 8mL, then regulating the pH value to 6.5 by using a 1M hydrochloric acid solution, stirring at 400rpm for 6.5h for chelation reaction, naturally cooling to room temperature after the reaction is finished, and performing suction filtration by using a Buchner funnel to obtain a small peptide chelate salt solution;

and S4, carrying out freeze drying on the small peptide chelate salt solution to obtain the small peptide chelate salt.

The sulfate is ferrous sulfate, copper sulfate, manganese sulfate and zinc sulfate according to a mass ratio of 1: 1: 2: 2.

The preparation method of the green grass powder comprises the following steps: the method comprises the following steps of mixing Chinese cabbage, alfalfa, chicory stem leaves and amaranthus viridis according to the mass ratio of 1: 1: 1: 1, uniformly mixing, crushing, and sieving by a 40-mesh sieve to obtain the green grass powder.

The polysaccharide additive is brown algae polysaccharide.

The preparation method of the brown algae polysaccharide comprises the following steps:

after being washed by water, the brown algae is placed in an oven at 50 ℃ for drying for 3d, taken out and crushed, and sieved by a 30-mesh sieve to obtain brown algae powder, wherein the ratio of the brown algae powder to the water is 1 g: 40mL of the mixture is mixed and soaked for 0.5h, then the mixture is heated and extracted for 2h at 100 ℃, the mixture is naturally cooled and filtered to obtain brown algae polysaccharide crude extract, the brown algae polysaccharide crude extract is subjected to rotary evaporation and concentration at 70 ℃ to 1/3 of the original volume to obtain concentrated solution, absolute ethyl alcohol with the volume 3 times that of the concentrated solution is added, the mixture is kept stand and precipitated for 1d at 4 ℃, the mixture is centrifuged at 3000rpm for 5min to remove supernatant fluid, alcohol precipitated solid is obtained, and the alcohol precipitated solid is placed in a 45 ℃ oven to be dried for 6h, so that brown algae polysaccharide is obtained.

Example 4

A preparation method of pig feed for improving immunity of organisms comprises the following steps:

1) mixing and stirring 100 parts of active ingredients, 30 parts of grass meal, 10 parts of polysaccharide additive, 0.3 part of vitamin complex, 0.6 part of garlicin and 15 parts of water uniformly according to parts by weight to obtain a premix;

2) and (3) putting the premix into a granulator for granulation to obtain the feed. The pig feed for improving the immunity of the organism is cylindrical particles, the particle size of the cylindrical particles is 0.6cm, the length of the cylindrical particles is 1.5cm, and the water content of the cylindrical particles is 12%.

The preparation method of the active ingredients comprises the following steps:

(1) uniformly mixing 40 parts of corn flour, 30 parts of sorghum flour, 20 parts of bran and 16 parts of bran coat according to parts by weight to obtain a coarse mixture;

(2) mixing the bean dregs, the bacillus subtilis, the crude mixture and a 2 wt% sodium chloride aqueous solution according to a material-liquid ratio of 15 g: 3 g: 85 g: mixing 60mL of the mixture to obtain a premix, placing the premix in a fermentation tank, and carrying out aerobic fermentation for 36 hours at 37 ℃ to obtain a main fermentation material;

(3) adding the fermentation main material and the premix in a mass ratio of 8: 100 of small peptide chelate salt, and uniformly mixing to obtain the active component.

The preparation method of the small peptide chelate salt comprises the following steps:

s1, cleaning shrimp shells, airing water, crushing, and screening by a 60-mesh sieve to obtain shrimp shell powder;

s2, placing the shrimp shell powder in water at 60 ℃, adjusting the pH to 9 by using 1M NaOH aqueous solution, adding alkaline protease for enzymolysis, wherein the feed-liquid ratio of the shrimp shell powder to the alkaline protease to the water is 40 g: 6 g: 110mL, performing enzymolysis for 8 hours, naturally cooling to room temperature, performing ultrasonic treatment at 300W and 30kHz for 25min to inactivate enzyme, filtering with four layers of common gauze of 100 meshes, and taking filtrate to obtain shrimp shell small peptide liquid;

s3, heating the shrimp shell small peptide liquid to 65 ℃ at a heating rate of 5 ℃/min, and then adding sulfate, wherein the ratio of the sulfate to the shrimp shell small peptide liquid is 1 g: 8mL, then regulating the pH value to 6.5 by using a 1M hydrochloric acid solution, stirring at 400rpm for 6.5h for chelation reaction, naturally cooling to room temperature after the reaction is finished, and performing suction filtration by using a Buchner funnel to obtain a small peptide chelate salt solution;

and S4, carrying out freeze drying on the small peptide chelate salt solution to obtain the small peptide chelate salt.

The sulfate is ferrous sulfate, copper sulfate, manganese sulfate and zinc sulfate according to a mass ratio of 1: 1: 2: 2.

The preparation method of the green grass powder comprises the following steps: the method comprises the following steps of mixing Chinese cabbage, alfalfa, chicory stem leaves and amaranthus viridis according to the mass ratio of 1: 1: 1: 1, uniformly mixing, crushing, and sieving by a 40-mesh sieve to obtain the green grass powder.

The polysaccharide additive is prepared from brown algae polysaccharide and nicandra physaloides seed polysaccharide in a mass ratio of 1: 2.

The preparation method of the nicandra physaloides seed polysaccharide comprises the following steps:

putting the nicandra physaloides seeds into water with the temperature of 40 ℃, stirring for 15min at 200rpm under the assistance of 500W and 25kHz ultrasonic waves, wherein the material-liquid ratio is 1 g: 35mL, separating the liquid phase and the solid phase by using a separator after stirring, taking the liquid phase, and freeze-drying to obtain the physalis pseudo-acid seed polysaccharide.

The preparation method of the brown algae polysaccharide comprises the following steps:

after being washed by water, the brown algae is placed in an oven at 50 ℃ for drying for 3d, taken out and crushed, and sieved by a 30-mesh sieve to obtain brown algae powder, wherein the ratio of the brown algae powder to the water is 1 g: 40mL of the mixture is mixed and soaked for 0.5h, then the mixture is heated and extracted for 2h at 100 ℃, the mixture is naturally cooled and filtered to obtain brown algae polysaccharide crude extract, the brown algae polysaccharide crude extract is subjected to rotary evaporation and concentration at 70 ℃ to 1/3 of the original volume to obtain concentrated solution, absolute ethyl alcohol with the volume 3 times that of the concentrated solution is added, the mixture is kept stand and precipitated for 1d at 4 ℃, the mixture is centrifuged at 3000rpm for 5min to remove supernatant fluid, alcohol precipitated solid is obtained, and the alcohol precipitated solid is placed in a 45 ℃ oven to be dried for 6h, so that brown algae polysaccharide is obtained.

Example 5

A preparation method of pig feed for improving immunity of organisms comprises the following steps:

1) mixing and stirring 100 parts of active ingredients, 30 parts of grass meal, 10 parts of polysaccharide additive, 0.3 part of vitamin complex, 0.6 part of garlicin and 15 parts of water uniformly according to parts by weight to obtain a premix;

2) and (3) putting the premix into a granulator for granulation to obtain the feed. The pig feed for improving the immunity of the organism is cylindrical particles, the particle size of the cylindrical particles is 0.6cm, the length of the cylindrical particles is 1.5cm, and the water content of the cylindrical particles is 12%.

The preparation method of the active ingredients comprises the following steps:

(1) uniformly mixing 40 parts of corn flour, 30 parts of sorghum flour, 20 parts of bran and 16 parts of bran coat according to parts by weight to obtain a coarse mixture;

(2) mixing the bean dregs, the bacillus subtilis, the crude mixture and a 2 wt% sodium chloride aqueous solution according to a material-liquid ratio of 15 g: 3 g: 85 g: 60mL of the premix was obtained, and the mixture was added in a mass ratio of 8: 100 small peptide chelate salt, uniformly mixing, placing in a fermentation tank, and carrying out aerobic fermentation at 37 ℃ for 36h to obtain the active ingredient.

The preparation method of the small peptide chelate salt comprises the following steps:

s1, cleaning shrimp shells, airing water, crushing, and screening by a 60-mesh sieve to obtain shrimp shell powder;

s2, placing the shrimp shell powder in water at 60 ℃, adjusting the pH to 9 by using 1M NaOH aqueous solution, adding alkaline protease for enzymolysis, wherein the feed-liquid ratio of the shrimp shell powder to the alkaline protease to the water is 40 g: 6 g: 110mL, performing enzymolysis for 8 hours, naturally cooling to room temperature, performing ultrasonic treatment at 300W and 30kHz for 25min to inactivate enzyme, filtering with four layers of common gauze of 100 meshes, and taking filtrate to obtain shrimp shell small peptide liquid;

s3, heating the shrimp shell small peptide liquid to 65 ℃ at a heating rate of 5 ℃/min, and then adding sulfate, wherein the ratio of the sulfate to the shrimp shell small peptide liquid is 1 g: 8mL, then regulating the pH value to 6.5 by using a 1M hydrochloric acid solution, stirring at 400rpm for 6.5h for chelation reaction, naturally cooling to room temperature after the reaction is finished, and performing suction filtration by using a Buchner funnel to obtain a small peptide chelate salt solution;

and S4, carrying out freeze drying on the small peptide chelate salt solution to obtain the small peptide chelate salt.

The sulfate is ferrous sulfate, copper sulfate, manganese sulfate and zinc sulfate according to a mass ratio of 1: 1: 2: 2.

The preparation method of the green grass powder comprises the following steps: the method comprises the following steps of mixing Chinese cabbage, alfalfa, chicory stem leaves and amaranthus viridis according to the mass ratio of 1: 1: 1: 1, uniformly mixing, crushing, and sieving by a 40-mesh sieve to obtain the green grass powder.

The polysaccharide additive is prepared from brown algae polysaccharide and nicandra physaloides seed polysaccharide in a mass ratio of 1: 2.

The preparation method of the nicandra physaloides seed polysaccharide comprises the following steps:

putting the nicandra physaloides seeds into water with the temperature of 40 ℃, stirring for 15min at 200rpm under the assistance of 500W and 25kHz ultrasonic waves, wherein the material-liquid ratio is 1 g: 35mL, separating the liquid phase and the solid phase by using a separator after stirring, taking the liquid phase, and freeze-drying to obtain the physalis pseudo-acid seed polysaccharide.

The preparation method of the brown algae polysaccharide comprises the following steps:

after being washed by water, the brown algae is placed in an oven at 50 ℃ for drying for 3d, taken out and crushed, and sieved by a 30-mesh sieve to obtain brown algae powder, wherein the ratio of the brown algae powder to the water is 1 g: 40mL of the mixture is mixed and soaked for 0.5h, then the mixture is heated and extracted for 2h at 100 ℃, the mixture is naturally cooled and filtered to obtain brown algae polysaccharide crude extract, the brown algae polysaccharide crude extract is subjected to rotary evaporation and concentration at 70 ℃ to 1/3 of the original volume to obtain concentrated solution, absolute ethyl alcohol with the volume 3 times that of the concentrated solution is added, the mixture is kept stand and precipitated for 1d at 4 ℃, the mixture is centrifuged at 3000rpm for 5min to remove supernatant fluid, alcohol precipitated solid is obtained, and the alcohol precipitated solid is placed in a 45 ℃ oven to be dried for 6h, so that brown algae polysaccharide is obtained.

Comparative example 1

Is a commercially available common pig feed which is purchased from Jinmengjie Biotechnology GmbH, and is a mixed fermentation feed with a grain size of 0.6cm, a length of 1.5cm and a water content of 12%.

Test example 1

The detection of the nutrient components is carried out according to GB/T5915-. The test results are shown in Table 1.

Table 1: results of nutrient component detection

	Crude protein/%)	Total amount of amino acids/%)	Crude fiber/%)
				Example 1	16.3	17.5	5.5
Example 4	15.8	16.9	5.1
				Example 5	17.6	20.8	4.2
Comparative example 1	26.3	18.4	23.3

Example 4 the percentage of protein content is slightly reduced, but within the standard range, due to the addition of the polysaccharide additive. Example 5 the small peptide chelate salt participates in fermentation, not only adds a nitrogen source required for enzyme production to bacillus subtilis, but also effectively promotes the enzyme production rate of metal ions in the bacillus subtilis, thereby improving the enzymolysis speed of crude fiber, reducing the content of the crude fiber, improving the content of crude protein, and enabling the feed to be more easily absorbed, digested and utilized.

The content of crude protein and crude fiber in the feed of the comparative example is much higher than that of the example, the proper amount of crude fiber is beneficial to the normal operation of the digestive system of the pig, but the excessive crude fiber can influence the palatability and the digestibility of the feed. The excessive protein content in the feed not only can cause the waste of protein resources, but also can influence the absorption and utilization of other nutrients due to the imbalance between the protein content and the distribution ratio of other nutrients. In addition, the animal and plant source mixed protein is more easily absorbed, digested and utilized after being processed, the use level of the protein is reduced, the utilization rate is improved, and the feed cost is reduced.

Because the protein sources are different, the amino acid structures are greatly different, and the nitrogen contents of different amino acids are also greatly different, the crude protein content of the comparative example is lower than that of the amino acid, and the crude protein content of the examples 1 and 4 is higher than that of the amino acid. Example 5 Small peptide chelated salt participates in fermentation, promotes the production of amino acid, and improves the ratio of total amino acid/crude protein.

Test example 2

And (3) carrying out sanitation index test on each prepared pig feed according to GB/T13078-.

The test of the content of the microbial pollutants specifically refers to GB/T13092-. The test results are shown in Table 2.

After the freshly prepared pig feed is stored in a shady and dry place in a sealed manner for 6 months, the microbial pollutant content test is carried out again, the storage conditions of all the pig feeds are the same, and the test results are shown in table 3.

Table 2: hygiene index test results

Table 3: hygiene index test results after 6 months of storage

Allicin has antibacterial effect, nicandra physaloides seed polysaccharide and brown algae polysaccharide have gel thickening effect, have coating effect on active ingredients of fermentation main materials, grass powder, vitamins and allicin, effectively thicken, keep fresh, preserve the activity of the active ingredients and prolong the storage life of the feed. The gel structure of the brown algae polysaccharide and the nicandra physaloides seed polysaccharide interpenetrates, the gel is more compact, and the preservation effect of the pig feed is synergistically improved.

Test example 3

Referring to GB/T26438-.

The test is divided into 3 stages of an adaptation period, a pre-feeding period and a positive test period, wherein the adaptation period and the pre-feeding period are 7d respectively, and the positive test period is 10 d. After the pilot period, the piglets were weighed and the average total weight gain was calculated, and the test results are shown in table 4.

Table 4: apparent digestion of pig feed can increase weight of piglets

	Apparent digestive energy (MJ/kg)	Average total weight gain (kg)
			Example 1	73.22	9.11
Example 2	79.03	10.56
			Example 3	80.72	10.63
Example 4	85.44	11.06
			Example 5	92.13	11.86
Comparative example 1	65.03	8.28

The pig feed for improving the immunity of the organism is a favorite taste of pigs, is beneficial to improving the ingestion frequency, can effectively adjust and maintain the microbial balance in the intestinal tracts of the pigs, and improves the immunity. In the embodiment 2, the embodiment 3 and the embodiment 4, the polysaccharide additive is added on the basis of the embodiment 1, so that satiety is increased, the stomach volume of piglets is small, the function of a digestive system is weak, and the digestion and absorption are facilitated when the piglets eat more than one meal, and the piglets grow healthily. In experiments, the unexpected discovery that the apparent digestion energy and the average total weight gain are obviously higher than the addition value of the action effect of the nicandra physaloides seed polysaccharide and the brown alga polysaccharide when the nicandra physaloides seed polysaccharide and the brown alga polysaccharide are compounded to be used as the polysaccharide additive, and the growth of pigs is accelerated. On one hand, the preservation effect of the effective active ingredients can be improved after compounding, so that the quality of the feed is kept stable in the testing process; on the other hand, the satiety caused by water absorption of the fed polysaccharide enables the pigs to eat more than one meal less, the brown algae polysaccharide promotes the cell growth, the feeding speed, the digestion and absorption speed and the cell growth speed are more prone to dynamic balance, and the conversion rate of the fed feed is improved, so the apparent digestion energy is high, and the weight gain is fast. The early stage of piglets mainly comprises the growth of bones and muscles, and the fat gain is relatively slow. The pig feed is rich in calcium and can promote bone growth. The shrimp shell collagen peptide is rich in plant and animal proteins, and the bean dregs are fermented, and the shrimp shell collagen is hydrolyzed into small peptide which is easier to absorb and digest and promotes the growth of muscles. And therefore gain weight faster than the comparative example. Example 5 the small peptide chelated salt is involved in fermentation, which can promote the production of beta-mannase, improve the energy digestibility of corn bean dregs and the like, improve the lean meat percentage, and thus promote the growth. In addition, part of small peptide chelate salt is converted into amino acid chelate salt, so that the biological value is higher, and the synthesis of protein and the absorption rate of trace elements are further improved.