阅读说明：本技术 一种快速止血液体创可贴及其制备方法 (Liquid band-aid capable of rapidly stopping bleeding and preparation method thereof ) 是由 邵恺元 李晓峰 金叙杭 谭博文 倪思文 王庆伟 余博 于 2021-09-14 设计创作，主要内容包括：本发明公开了一种快速止血液体创可贴及其制备方法,包含如下步骤：制备改性的墨鱼骨粉；取18～36份去离子水,在其中加入2～4份聚乙烯醇；溶胀后,将其逐渐加热到90～100℃,再加入2～4份聚乙烯醇,溶解后降温至55～65℃,得到第一溶液；将0.05～1份羧甲基纤维素钠、0.05～1份聚氧化乙烯、1～5份海藻酸钠和余下的2～4份聚乙烯醇混合并倒入容器,再加入42～84份去离子水,水浴加热至粉末完全溶解,降温至55～65℃,得到第二溶液；在55～65℃的温度下,将第二溶液缓慢加入第一溶液中,并加入0.05～1份抑菌剂、2～6份生物粘附剂、1～3份苯甲醇、1～7份甘油,得到第三溶液；在第三溶液中加入改性墨鱼骨粉0.05～1份,然后进行高压蒸汽灭菌,得到液体创可贴。本发明制备的液体创可贴无毒、无刺激性,适于伤口愈合。(The invention discloses a liquid band-aid capable of rapidly stopping bleeding and a preparation method thereof, wherein the liquid band-aid comprises the following steps: preparing modified cuttlefish bone meal; taking 18-36 parts of deionized water, and adding 2-4 parts of polyvinyl alcohol into the deionized water; after swelling, gradually heating the mixture to 90-100 ℃, adding 2-4 parts of polyvinyl alcohol, dissolving, and cooling to 55-65 ℃ to obtain a first solution; mixing 0.05-1 part of sodium carboxymethylcellulose, 0.05-1 part of polyethylene oxide, 1-5 parts of sodium alginate and the rest 2-4 parts of polyvinyl alcohol, pouring into a container, adding 42-84 parts of deionized water, heating in a water bath until the powder is completely dissolved, and cooling to 55-65 ℃ to obtain a second solution; slowly adding the second solution into the first solution at the temperature of 55-65 ℃, and adding 0.05-1 part of bacteriostatic agent, 2-6 parts of biological adhesive, 1-3 parts of benzyl alcohol and 1-7 parts of glycerol to obtain a third solution; and adding 0.05-1 part of modified cuttlefish bone meal into the third solution, and then performing high-pressure steam sterilization to obtain the liquid band-aid. The liquid band-aid prepared by the invention is nontoxic, nonirritant and suitable for wound healing.)

1. A preparation method of a liquid woundplast for rapidly stopping bleeding is characterized by comprising the following steps:

s1, preparing modified cuttlefish bone meal: coating a layer of surfactant on the surface of the cuttlefish bone meal;

s2, taking 18-36 parts by mass of deionized water, adding 2-4 parts of polyvinyl alcohol, gradually heating to 90-100 ℃ after swelling, adding 2-4 parts of polyvinyl alcohol after the polyvinyl alcohol is completely dissolved, cooling to 55-65 ℃ after the polyvinyl alcohol is completely dissolved, and maintaining the temperature to obtain a first solution;

s3, mixing and grinding 0.05-1 part of sodium carboxymethylcellulose, 0.05-1 part of polyethylene oxide, 1-5 parts of sodium alginate and the rest 2-4 parts of polyvinyl alcohol into powder, pouring the powder into a container, adding the rest 42-84 parts of deionized water, heating in a water bath until the powder is completely dissolved, cooling to 55-65 ℃, and maintaining the temperature to obtain a second solution;

s4, slowly adding the second solution into the first solution at the temperature of 55-65 ℃, and adding 0.05-1 part of bacteriostatic agent, 2-6 parts of biological adhesive, 1-3 parts of benzyl alcohol and 1-7 parts of glycerol; stirring uniformly to obtain a third solution;

s5, adding 0.05-1 part of the modified cuttlefish bone meal prepared in the step S1 into the third solution, uniformly stirring, treating with ultrasonic waves, and then performing high-pressure steam sterilization to obtain the liquid band-aid.

2. The method for preparing a liquid wound patch for rapid hemostasis as claimed in claim 1, wherein the steps are

The step of modifying cuttlefish bone meal in S1 includes:

s1.1, grinding and sieving commercially available cuttlefish bone meal;

s1.2, placing the cuttlefish bone meal ground in the step S1.1 in a weak acid buffer solution, stirring, filtering and washing for multiple times until the filtrate is neutral;

s1.3, placing the cuttlefish bone meal obtained in the step S1.2 in a surfactant solution, stirring, standing for 8-12 hours, and then treating with ultrasonic waves for 0.5-1 hour to obtain a suspension;

s1.4, centrifuging the suspension obtained in the step S1.3, and placing the obtained precipitate in a dryer for 12-48 hours to obtain the modified cuttlefish bone meal.

3. The method for preparing the liquid woundplast for rapid hemostasis as claimed in claim 1, wherein: in step S1, tween-40 is selected as the surfactant.

4. The method for preparing the liquid woundplast for rapid hemostasis as claimed in claim 1, wherein: in step S4, the bioadhesive is gelatin or aqueous polyurethane.

5. The method for preparing the liquid woundplast for rapid hemostasis as claimed in claim 4, wherein: the waterborne polyurethane is anionic or nonionic waterborne polyurethane.

6. The method for preparing the liquid woundplast for rapid hemostasis as claimed in claim 4, wherein: the hard segment of the waterborne polyurethane is made of isophorone diisocyanate.

7. The method for preparing the liquid woundplast for rapid hemostasis as claimed in claim 1, wherein: the polyvinyl alcohol is PVA-2399.

8. A quick hemostasis liquid band-aid prepared by the method of any one of claims 1 to 7, which is characterized by comprising the following raw materials in parts by mass: 6-12 parts of polyvinyl alcohol, 1-5 parts of sodium alginate, 0.05-1 part of carboxymethyl cellulose, 0.05-1 part of polyoxyethylene, 1-7 parts of glycerol, 2-6 parts of a biological adhesive, 0.05-1 part of a bacteriostatic agent, 0.05-1 part of modified cuttlefish bone meal, 1-3 parts of benzyl alcohol and 60-120 parts of deionized water.

Technical Field

The invention relates to the technical field of medical supplies, in particular to a liquid band-aid capable of rapidly stopping bleeding and a preparation method thereof.

Background

A wound pad is the most commonly used medical device in people's lives for treating wounds. The traditional band-aid is mainly composed of a strip-shaped adhesive tape, a small piece of gauze soaked with benzalkonium chloride is attached to the middle of the strip-shaped adhesive tape, and the gauze is attached to a wound to play roles in protecting the wound, absorbing exudates, temporarily stopping bleeding and the like. Its advantages are low cost, small size and convenient carrying about. However, they have obvious disadvantages such as poor water resistance, poor air permeability, and failure to fit well to the skin at the joints.

Liquid bandages (liquid dressings) are a new type of wound dressing that has emerged at the end of the 20 th century, at the beginning of the 21 st century. It is in liquid form before use, and when applied to a wound, it rapidly forms a protective film on the wound by physical or chemical means and provides a suitable healing environment for the wound. Compared with the traditional band-aid, the liquid band-aid has the advantages of strong waterproofness, good air permeability, antibiosis, attractive appearance, scar reduction and the like. However, some of the liquid band-aids on the market also have the disadvantages of strong irritation and pungent smell. Taking a good-selling-amount liquid woundplast for Japan and German SOS brands as examples, the film-forming materials are respectively low-nitrogen cellulose nitrate and acrylates, the solvents are ethyl acetate, butyl acetate and the like, and the film-forming materials have strong irritation to wounds, particularly, the low-nitrogen cellulose nitrate can bring great pain to the wounds, namely Wangtianyu, Zhang Meijing, House Machil, the rest, Songhong waves, research progress of the waterproof liquid woundplast [ J ] new medicine, New medicine, Chinese J2016, 25 (04): 433 and 438.

Therefore, it is an urgent problem to develop a liquid adhesive bandage which has no irritation to wound, strong hemostatic property, antibacterial property and no pungent smell to meet the requirement of people on wound treatment.

Disclosure of Invention

The invention aims to overcome the defects of strong irritation and the like in the existing liquid band-aid, and provides the quick hemostasis liquid band-aid which has no irritation, can quickly stanch, has good water resistance and air permeability, good elasticity, transparent color after film forming and can be well adhered to skin.

In order to achieve the above object, the present invention provides a method for preparing a liquid woundplast for rapid hemostasis, comprising the following steps:

s1, preparing modified cuttlefish bone meal: coating a layer of surfactant on the surface of the cuttlefish bone meal;

s2, taking 18-36 parts by mass of deionized water, adding 2-4 parts of polyvinyl alcohol, gradually heating to 90-100 ℃ after swelling, adding 2-4 parts of polyvinyl alcohol after the polyvinyl alcohol is completely dissolved, cooling to 55-65 ℃ after the polyvinyl alcohol is completely dissolved, and maintaining the temperature to obtain a first solution;

s3, mixing and grinding 0.05-1 part of sodium carboxymethylcellulose, 0.05-1 part of polyethylene oxide, 1-5 parts of sodium alginate and the rest 2-4 parts of polyvinyl alcohol into powder, pouring the powder into a container, adding the rest 42-84 parts of deionized water, heating in a water bath until the powder is completely dissolved, cooling to 55-65 ℃, and maintaining the temperature to obtain a second solution;

s4, slowly adding the second solution into the first solution at the temperature of 55-65 ℃, and adding 0.05-1 part of bacteriostatic agent, 2-6 parts of biological adhesive, 1-3 parts of benzyl alcohol and 1-7 parts of glycerol; stirring uniformly to obtain a third solution;

s5, adding 0.05-1 part of the modified cuttlefish bone meal prepared in the step S1 into the third solution, uniformly stirring, treating with ultrasonic waves, and then performing high-pressure steam sterilization to obtain the liquid band-aid.

Preferably, the step of modifying the cuttlefish bone meal in step S1 includes:

s1.1, grinding and sieving commercially available cuttlefish bone meal;

s1.2, placing the cuttlefish bone meal ground in the step S1.1 in a weak acid buffer solution, stirring, filtering and washing for multiple times until the filtrate is neutral;

s1.3, placing the cuttlefish bone meal obtained in the step S1.2 in a surfactant solution, stirring, standing for 8-12 hours, and then treating with ultrasonic waves for 0.5-1 hour to obtain a suspension;

s1.4, centrifuging the suspension obtained in the step S1.3, and placing the obtained precipitate in a dryer for 12-48 hours to obtain the modified cuttlefish bone meal.

Preferably, in step S1, tween-40 is selected as the surfactant.

Preferably, in step S4, the bioadhesive is gelatin or aqueous polyurethane.

Preferably, the aqueous polyurethane is anionic or nonionic aqueous polyurethane.

Preferably, the hard segment of the waterborne polyurethane is selected from isophorone diisocyanate.

Preferably, the polyvinyl alcohol is PVA-2399.

The invention also discloses a quick hemostasis liquid band-aid prepared by using the method, which comprises the following raw materials in parts by weight: 6-12 parts of polyvinyl alcohol, 1-5 parts of sodium alginate, 0.05-1 part of carboxymethyl cellulose, 0.05-1 part of polyoxyethylene, 1-7 parts of glycerol, 2-6 parts of a biological adhesive, 0.05-1 part of a bacteriostatic agent, 0.05-1 part of modified cuttlefish bone meal, 1-3 parts of benzyl alcohol and 60-120 parts of deionized water.

Compared with the prior art, the liquid band-aid provided by the invention has the beneficial effects that:

(1) the material and the solvent required by the preparation of the liquid band-aid are nontoxic and nonirritating;

(2) the sodium alginate can improve the hemostatic performance and the air permeability of the liquid band-aid and can improve the tensile strength of the liquid band-aid after film forming;

(3) calcium carbonate, colloid and organic matters contained in the cuttlefish bone meal have the hemostatic effect and promote wound healing;

(4) the biological adhesive such as water-based polyurethane, gelatin and the like improves the water contact angle of the polyvinyl alcohol film, so that the polyvinyl alcohol film is more suitable for wound healing.

Drawings

FIG. 1 is a flow chart of a method of making a liquid band-aid of the present invention;

FIG. 2 is a line graph showing the swelling of the liquid adhesive bandage obtained in example 1 in physiological saline after forming a film;

fig. 3 is a schematic diagram of the water contact angle of the liquid woundplast obtained in example 1 after film formation.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "first", "second", and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

The invention discloses a liquid band-aid capable of rapidly stopping bleeding, which is composed of the following raw materials in parts by weight: 6-12 parts of polyvinyl alcohol, 1-5 parts of sodium alginate, 0.05-2 parts of sodium carboxymethylcellulose, 0.05-2 parts of polyoxyethylene, 1-7 parts of glycerol, 2-6 parts of a biological adhesive, 0.05-1 part of a bacteriostatic agent, 0.05-1 part of modified cuttlefish bone meal, 1-3 parts of benzyl alcohol and 60-120 parts of deionized water.

The preparation method of the quick hemostasis liquid band-aid is shown in figure 1 and comprises the following steps:

s1, preparing modified cuttlefish bone meal; coating a layer of surfactant on the surface of the cuttlefish bone meal;

s2, firstly, taking 18-36 parts of deionized water, adding 2-4 parts of polyvinyl alcohol into the deionized water, and swelling; swelling for half an hour, gradually heating the mixture to 90-100 ℃ until the polyvinyl alcohol is completely dissolved, then adding 2-4 parts of polyvinyl alcohol, cooling to 55-65 ℃ after the polyvinyl alcohol is completely dissolved, and maintaining the temperature to obtain a first solution;

s3, mixing and grinding 0.05-2 parts of sodium carboxymethylcellulose, 0.05-2 parts of polyethylene oxide, 1-5 parts of sodium alginate and the rest 2-4 parts of polyvinyl alcohol into powder, pouring the powder into a sealable container, adding the rest 42-84 parts of deionized water, sealing the container, heating the container in a water bath at 100 ℃ until the powder is completely dissolved, cooling to 55-65 ℃, and maintaining the temperature to obtain a second solution;

s4, slowly adding the second solution into the first solution at the temperature of 55-65 ℃, and adding 0.05-1 part of bacteriostatic agent, 2-6 parts of biological adhesive, 1-3 parts of benzyl alcohol and 1-7 parts of glycerol; stirring uniformly to obtain a third solution;

s5, adding 0.05-1 part of the modified cuttlefish bone meal prepared in the step S1 into the third solution, stirring the mixture by using a mechanical stirrer at the rotating speed of 60-120 rmp until the mixture is uniformly stirred, treating the mixture for 1 hour by using ultrasonic waves, putting the mixture into a high-pressure steam cooker for sterilization, and sterilizing the mixture for 30 minutes at the pressure of 103.4kPa and the temperature of 121 ℃ to obtain the liquid band-aid.

In S2-S4, polyvinyl alcohol and deionized water are added step by step, because the finished product of the liquid band-aid is thick, if all raw materials are added at one time, the phenomena of incomplete dissolution, bottom pasting, slow dissolution and the like can occur, and the raw materials are added step by step to control the viscosity, so that the operation is convenient. The reason for separately preparing and then mixing the first solution and the second solution is not only to control the viscosity but also to avoid precipitation of the bioadhesive at high temperatures.

Preferably, the polyvinyl alcohol is selected from one or a mixture of PVA1799 and PVA2399, and the polyvinyl alcohol with alcoholysis degree of 99% is difficult to dissolve in cold water and easy to dissolve in hot water with a temperature of more than 90 ℃, so that the polyvinyl alcohol can be dissolved in water under a heating condition, and the finally obtained liquid band-aid has water resistance at normal temperature, and is not easy to fall off when being applied to hands for washing hands; further, PVA2399 was selected because PVA2399 has a higher degree of polymerization and a higher strength.

The addition of sodium alginate into the formula of the liquid band-aid improves the film forming property and greatly enhances the hemostatic property of the liquid band-aid. The guluronic acid component in the sodium alginate can react with calcium ions, iron ions and the like in blood exuded from wounds to crosslink, and is quickly cured at bleeding parts, so that the liquid band-aid has very good hemostatic performance. The addition of sodium alginate can also improve the air permeability of the liquid band-aid film, and is more beneficial to wound healing.

After sodium carboxymethylcellulose is added into sodium alginate, the two macromolecules can generate the interaction of hydrogen bonds and the like, so that the tensile strength of the liquid adhesive bandage is improved, and the same effect can be generated by adding polyoxyethylene.

The glycerol is a plasticizer and is used for improving the elongation at break of the film after the liquid band-aid is formed into the film.

The benzyl alcohol is a preservative and is used for preventing the liquid band-aid from deteriorating before use.

The surfactant is selected from gelatin or waterborne polyurethane, and the waterborne polyurethane is preferred because the waterborne polyurethane has better adhesiveness; further, the aqueous polyurethane is anionic or nonionic aqueous polyurethane, because cationic aqueous polyurethane reacts with sodium alginate with anions and precipitates. The hard segment of the waterborne polyurethane is preferably isophorone diisocyanate, and compared with other isocyanates, the isophorone diisocyanate is lowest in toxicity. Further, the polyurethane is treated by using a blocking agent to eliminate free isocyanate, because the isocyanate has stronger toxicity; the end-capping reagent can be ethanol or ethylene glycol. Sampling and detecting, wherein when the waterborne polyurethane is detected by a toluene/di-n-butylamine-hydrochloric acid titration method in GB/T12009.4-2016, the existence of isocyanic acid radical cannot be detected.

Preferably, the bacteriostatic agent is one of chlorhexidine gluconate or benzalkonium chloride; further, benzalkonium chloride is preferable.

The traditional Chinese medicine component cuttlefish bone powder is added into the formula of the liquid adhesive bandage. Calcium carbonate contained in cuttlefish bone powder can promote inhibition of wound surface inflammation, stop bleeding, and relieve local pain. The colloid and organic matter in the cuttlefish bone powder can be used for coagulating blood and promoting wound healing.

In step S1, the purpose of the modification of the cuttlefish bone powder is to coat a layer of surfactant on the surface of the cuttlefish bone powder so that the cuttlefish bone powder can be easily suspended in the solution. The surfactant is Tween-40 (polysorbate-40), and because Tween-40 has low viscosity, the modified dispersion effect in water can be improved, and the modification of cuttlefish bone meal is facilitated. The modification step comprises the following steps:

s1.1, grinding commercially available cuttlefish bone meal until the sieving rate of 400 meshes is more than 95%, and sieving;

s1.2, placing the cuttlefish bone meal obtained in the step S1.1 in an acetic acid-sodium acetate buffer solution with the concentration of 0.11% and the buffer ratio of 1: 1, stirring at the speed of 60-120 rpm for 5-10 minutes, filtering, washing with deionized water, and filtering for multiple times until the pH value of the filtrate is 7; the purpose of treating with weak acid buffer solution is to clean the cuttlefish bone powder and corrode the surface of the cuttlefish bone powder to form a porous structure, so that the cuttlefish bone powder is conveniently coated with a surfactant;

s1.3, placing the cuttlefish bone meal obtained in the step S1.2 in a surfactant with the concentration of 5-15% (in the example, a Tween-40 solution), stirring at the speed of 60-120 rpm for 5-10 minutes, standing overnight, and treating with ultrasonic waves for 1 hour to obtain a suspension;

s1.4, putting the suspension obtained in the step S1.3 into a centrifugal tube, centrifuging at the rotating speed of 6000rpm for 3 minutes, taking the precipitate at the lower part of the centrifugal tube, and placing in a dryer for 24 hours to obtain the modified cuttlefish bone meal; the centrifugal method has the advantages that the centrifugal method has high efficiency in preparation, and the coating layer of the surfactant is not easy to damage.

The technical effects of the present invention are illustrated below by specific examples and comparative examples.

Example 1

S1, preparing modified cuttlefish bone meal;

s2, 30g of deionized water was taken, and 3.4g of polyvinyl alcohol PVA-2399 was added thereto. Swelling for half an hour, gradually heating to 95 deg.C until polyvinyl alcohol is completely dissolved, adding 3.3g polyvinyl alcohol PVA-2399, completely dissolving, cooling to 60 deg.C, and maintaining the temperature;

s3, mixing and grinding 3.3g of polyvinyl alcohol PVA-2399, 0.6g of sodium alginate, 0.06g of sodium carboxymethylcellulose and 0.06g of polyethylene oxide into powder, pouring the powder into a sealable container, adding 60g of deionized water, sealing the container, heating the container in a water bath at 100 ℃ until the powder is completely dissolved, cooling to 60 ℃ and maintaining the temperature;

s4, slowly adding the solution prepared in the step S3 into the solution prepared in the step S2 at 60 ℃, adding 0.1g of bacteriostatic agent, 4g of aqueous polyurethane solution, 2g of benzyl alcohol and 2g of glycerol, and uniformly stirring;

s5, adding 0.05g of modified cuttlefish bone meal into the solution prepared in the step S4, stirring the mixture by using a mechanical stirrer at the rotating speed of 60-120 rmp until the mixture is uniformly stirred, treating the mixture for 1 hour by using ultrasonic waves, putting the mixture into a high-pressure steam cooker for sterilization, and sterilizing the mixture for 30 minutes at the pressure of 103.4kPa and the temperature of 121 ℃ to obtain the liquid band-aid.

Example 2

In this example, S1, S2, S4 and S5 are the same as in example 1.

S3, mixing and grinding 3.3g of polyvinyl alcohol PVA-2399, 1.25g of sodium alginate, 0.125g of sodium carboxymethylcellulose and 0.125g of polyethylene oxide into powder, pouring the powder into a sealable container, adding 60g of deionized water, sealing the container, heating the container in a water bath at 100 ℃ until the powder is completely dissolved, cooling to 60 ℃ and maintaining the temperature.

Example 3

In this example, S1, S2, S4 and S5 are the same as in example 1.

S3, mixing and grinding 3.3g of polyvinyl alcohol PVA-2399, 2.5g of sodium alginate, 0.25g of sodium carboxymethylcellulose and 0.25g of polyethylene oxide into powder, pouring the powder into a sealable container, adding 60g of deionized water, sealing the container, heating the container in a water bath at 100 ℃ until the powder is completely dissolved, cooling to 60 ℃ and maintaining the temperature.

Example 4

In this example, S1 to S3 and S5 are the same as those in example 1.

S4, slowly adding the solution prepared in the step S3 into the solution prepared in the step S2 at 60 ℃, adding 0.1g of bacteriostatic agent, 1g of gelatin (powder), 2g of benzyl alcohol and 2g of glycerol, and uniformly stirring.

Example 5

In this example, S1 to S3 and S5 are the same as in example 2, and S4 is the same as in example 4.

Example 6

In this example, S1 to S3 and S5 are the same as in example 3, and S4 is the same as in example 4.

Comparative example

12g of polyvinyl alcohol PVA2399 are added to 90g of deionized water, and after swelling for half an hour, the mixture is gradually heated to 95 ℃ until the polyvinyl alcohol is completely dissolved.

The performance of the pastes in examples 1-6 and comparative examples were then tested.

(1) After the liquid woundplast is dried into a film, the mechanical property of the film is tested

The above film was formed into a dumbbell-shaped specimen in accordance with GB/T528-2009, wherein the total length was 75mm, the width of the end portion was 12.5mm, the length of the narrow portion was 25mm, and the width of the narrow portion was 4mm), and a tensile test was conducted with a universal material testing machine at a pull-up speed of 50 mm/min. The mechanical properties of the films were evaluated by calculating the tensile strength and elongation at break of the films.

The tensile strength σ was calculated by the following formula_b：

σ_b＝F_b/S_o

In the formula: f_bThe maximum force to which the material is subjected when it breaks; s_oIs the original cross-sectional area of the narrow portion of the dumbbell-shaped membrane.

The elongation at break E was calculated using the formula:

E＝(L’-L)/L

in the formula: l is the original length of the material; l' is the length at break after deformation by force.

The measurement results are shown in table 1:

TABLE 1 measurement results of tensile Strength and elongation at Break of examples and comparative examples

As can be seen from table 1, examples 1, 2, 4 and 5 have excellent mechanical properties.

After the aqueous polyurethane, the gelatin, the sodium alginate and the sodium carboxymethyl cellulose are added, the mechanical strength of the liquid band-aid is higher than that of pure polyvinyl alcohol. This is because, when a small amount of the above-mentioned material is added, it has good compatibility with polyvinyl alcohol and undergoes hydrogen bond interaction, which enhances the strength of the film.

With the addition of more sodium alginate and sodium carboxymethyl cellulose, the mechanical strength of the liquid band-aid is reduced. The compatibility of sodium alginate, sodium carboxymethylcellulose and the like with polyvinyl alcohol is poor along with the increase of the content of sodium alginate, sodium carboxymethylcellulose and the like, so that the number of pores in the film is increased, when the number of pores is too large, a stress concentration phenomenon is easily caused under the stretching of an external force, and at the moment, the tensile strength and the elongation at break of the film are obviously reduced.

(2) Test of adhesion, hemostasis and anti-infection effects of liquid band-aid

14 healthy C57 mice were selected, divided into 7 groups, and the hair on the back was shaved off, and a 8mm long wound was made on each of the left and right sides of the back of the mice to the dermis with a scalpel. In which the left wounds of all mice were treated with physiological saline only as a control experiment, and the right wounds of each group of mice were smeared with the liquid wound dressings prepared in 6 examples and 1 control example, respectively. The hemostasis time of the wound of the mouse, the adhesion effect of the liquid band-aid after 24 hours, whether the wound is infected with suppuration and the like are observed.

Wounds on the left side of the mice generally stop bleeding by themselves after 25 minutes, and all wounds on the left side of the mice scab after 24 hours, wherein 6 wounds have more obvious suppuration symptoms.

The wounds on the right side of the mouse were stopped almost immediately after the application of the liquid band-aids prepared in the 6 examples, while the average stopping time was 45 seconds after the application of the control example. After 24 hours, the liquid wound dressing films of example 3 and the control example were peeled off the wound, and the films of the remaining groups were still well adhered. Careful observation showed that no symptoms of wound scabbing and suppuration occurred in any of the 6 groups of the wound dressing example. The wound applied in the control example had scabbed, but no obvious suppuration symptom occurred. The details are shown in Table 2.

TABLE 2 medical Effect of examples and comparative examples

Numbering	Hemostatic effect	Anti-infective effect	Skin adhesion
				Example 1	Good effect	Good effect	Good effect
Example 2	Good effect	Good effect	Good effect
				Example 3	Good effect	Good effect	Is poor
Example 4	Good effect	Good effect	Good effect
				Example 5	Good effect	Good effect	Good effect
Example 6	Good effect	Good effect	Good effect
				Comparative example	Is poor	In general	Is poor

As is clear from Table 2, examples 1 to 6 all had good hemostatic and anti-infective properties, and examples 1 to 2 and examples 4 to 6 all had excellent skin adhesion properties. The cuttlefish bone powder serving as a traditional Chinese medicinal material has hemostatic property, the guluronic acid component in a sodium alginate molecular chain can generate an ion exchange reaction after contacting with calcium ions and ferrous ions in human blood, and guluronic acid units are stacked to form a cross-linked network structure. Thereby leading the liquid band-aid to be quickly solidified on the surface of the wound to form hydrogel and having good hemostatic effect.

(3) Test of film forming time of liquid woundplast solution on human skin surface

The liquid band-aid was applied to the skin of the arm of a subject (the average temperature of the epidermis of the human body was 34.5 ℃ and the relative humidity of the environment was 50%), the time required for drying to form a film was measured, and the average value was taken.

The average film forming time of the liquid woundplast is shown in table 3:

TABLE 3 average film-Forming time of liquid band-aid

As can be seen from Table 3, the liquid adhesive bandage prepared by adding polyurethane into the raw materials is faster in drying and film forming, and is more beneficial to protecting the wound in time within 5-6 minutes; the addition of gelatin to the raw material is somewhat slow, whereas pure polyvinyl alcohol solutions are difficult to dry.

(4) Swelling performance test of liquid woundplast film

Cutting the liquid adhesive bandage film sample into 4cm × 2cm rectangles, putting the rectangles into a dryer for 12 hours to completely dry the liquid adhesive bandage film sample, and recording the mass of the liquid adhesive bandage film sample as W_oThen, the film was immersed in 0.9% physiological saline, and the mass of the film was weighed at 10 th, 20 th, 40 th, 60 th, 90 th, 120 th, 150 th, 180 th, 210 th, and 240 th min, respectively, and fig. 2 shows the change in the weight gain percentage with the immersion time in the case of immersion in 0.9% physiological saline in example 1, and it can be seen that the mass of the liquid band-aid did not substantially change after immersion for 240min, and the mass measured at 240min was denoted as W.

The swell gain was calculated using the formula:

the results are shown in Table 4.

TABLE 4 swelling weight gain ratio of liquid band-aid

The liquid band-aid is generally required to have higher swelling rate as a wound dressing, so that the wound dressing can absorb a small amount of wound exudate through swelling, and is beneficial to healing of a wound surface. According to the data, the swelling performance of the liquid adhesive bandage film can be enhanced by gradually adding sodium alginate and sodium carboxymethylcellulose into the PVA matrix, the insolubility of the liquid adhesive bandage film can be reduced with the addition of soluble substances such as sodium alginate, and when a large amount of sodium alginate is added, the liquid adhesive bandage film tends to swell first and then dissolve. From the contents of table 4, it is understood that the swelling properties of examples 1, 2, 4 and 5 are the most excellent.

(5) Surface hydrophilicity test of liquid band-aid film

The hydrophilic property of the liquid adhesive bandage film is evaluated by measuring the water contact angle of the liquid adhesive bandage film. As shown in FIG. 3, photographs of the contact between deionized water and the liquid wound dressing film were taken by a water contact angle measuring instrument (model: JC2000D), and the water contact angles were analyzed by software as shown in Table 5:

TABLE 5 Water contact Angle of liquid Woundplast film

Numbering	Water contact angle
		Example 1	78.5°
Example 2	74.0°
		Example 3	65.0°
Example 4	36.5°
		Example 5	35.5°
Example 6	35.0°
		Comparative example	17.0°

In the process of wound healing, cell adhesion and proliferation depend largely on the surface properties of the material to which the cells adhere. It is generally believed that a wound dressing having a water contact angle in the range of 60 ° to 80 ° is most suitable for wound healing. Biological adhesives such as water-based polyurethane, gelatin and the like are added into the PVA matrix, so that the water contact angle of the surface of the liquid woundplast film can be improved, and the defect that the surface of the PVA matrix is too hydrophilic and is not suitable for cell attachment is overcome. Since the solubility of the aqueous polyurethane is less than that of gelatin, the water contact angles of examples 1-3 are significantly higher than those of examples 4-6. Examples 1, 2, 3 are the most cell attachment friendly.

(6) Infrared spectroscopic analysis of liquid band-aid

And measuring the infrared spectrum of the liquid woundplast film by a potassium bromide tabletting method. Grinding 20 mg liquid woundplast film and 500 mg spectrum grade potassium bromide into powder, oven drying, pressing into sheet, and measuring with Fourier infrared spectrometer (model: VERTEX 70) at 4000cm^-1～400cm^-1Scanning in a range with a scanning resolution of 2cm^-1。

The infrared spectrum measurement result is as follows: all liquid woundplast films were at 3400cm^-1～3150cm^-1Both broad absorption peaks, which are due to glycerol and polyvinyl alcohol in the filmDue to hydroxyl groups; at 2940cm^-1～2900cm^-1All have a relatively sharp absorption peak due to the-CH of the polyvinyl alcohol in the film₂-due to the stretching vibrations of the skeleton. In addition, all fingerprint regions are obviously characterized by polyvinyl alcohol; 1648cm in the film sample with the addition of the aqueous polyurethane^-1A carbonyl absorption peak appears in the vicinity.

(7) Scanning Electron Microscope (SEM) analysis of liquid band-aid

A sample of the liquid adhesive bandage film was cut into filaments having a width of about 0.5mm, stuck on a conductive adhesive, and subjected to a sputtering gold-spraying treatment by a scanning electron microscope (model: EM-30AX) for observation. The magnification is 200 times, 500 times and 1000 times respectively. Observing the change of the surface appearance of the liquid band-aid after film forming to obtain that:

polyvinyl alcohol, sodium alginate, sodium carboxymethyl cellulose and other materials are completely dissolved to form a continuous phase.

After the water polyurethane and the gelatin are added, the surface has higher porosity, so that the water vapor passing rate of the liquid woundplast after film forming is improved. Particularly, after the water-based polyurethane is added, the network density of the polyvinyl alcohol is reduced, and the defects caused by high crystallinity of the polyvinyl alcohol are improved.

After the water polyurethane is added, the surface is shrunk to form a regular texture, which obviously influences the mechanical property of the film and improves the elasticity of the film during stretching.

The 7 groups of experiments show that the examples 1-6 have good hemostatic performance; example 1, example 2, example 4, example 5, example 6 had good skin adhesion; examples 1, 2, 4 and 5 have excellent mechanical properties and higher swelling properties; examples 1, 2 and 3 have suitable hydrophilicity. Combining the above experimental data, the liquid band-aid obtained in examples 1 and 4 has the best performance, wherein example 1 is better.

In conclusion, the liquid band-aid provided by the invention has higher strength after being filmed, and if the tensile strength is up to 60MPa, the liquid band-aid has strong elasticity; compared with other film forming materials and liquid band-aid prepared by organic solvent, the irritation is low; the sodium alginate can be used as a hemostatic agent, so that the mechanical property of the liquid band-aid film after film formation is improved, and the air permeability of the film is improved; the modified cuttlefish bone powder can be used for stopping bleeding, diminishing inflammation, and promoting wound healing; the water-based polyurethane can enhance the adhesion effect on skin and improve the hydrophilic property of the polyvinyl alcohol.

While the present invention has been described in detail with reference to the preferred embodiments, it should be understood that the above description should not be taken as limiting the invention. Various modifications and alterations to this invention will become apparent to those skilled in the art upon reading the foregoing description. Accordingly, the scope of the invention should be determined from the following claims.