阅读说明：本技术 带有对称参比电极的封闭式双极电极阵列的分析检测装置 (Analysis and detection device of closed bipolar electrode array with symmetrical reference electrodes ) 是由 蒋星宇 张加栋 俞伟渐 肖赟 周苏闽 朱秀芳 李进 韩海峰 赵建强 张大兵 于 2021-08-18 设计创作，主要内容包括：本发明涉及分析检测技术领域,公开了一种带有对称参比电极的封闭式双极电极阵列的分析检测装置,包括多个对称设置在承载层上的反应池A阵列、反应池B阵列,反应池A包括双极电极的端点A、驱动电极A以及参比电极A,反应池B包括双极电极的端点B、驱动电极B以及参比电极B；所述端点A和端点B仅通过承载层上的导电层中位于两端点之间的导电条带阵列互相连接,驱动电极A、参比电极A、驱动电极B以及参比电极B通过所述导电层与外界电源连接。本发明采用拥有对称参比电极的封闭式双极电极阵列可有效地解决现有封闭式双极电极在电沉积、电化学修饰端点A阵列和端点B阵列时难以精准控制的问题,拓展了双极电极在分析检测领域上的应用前景。(The invention relates to the technical field of analysis and detection, and discloses an analysis and detection device of a closed bipolar electrode array with symmetrical reference electrodes, which comprises a plurality of reaction pool A arrays and reaction pool B arrays, wherein the reaction pool A arrays and the reaction pool B arrays are symmetrically arranged on a bearing layer; the end points A and B are only connected with each other through a conductive strip array positioned between the two end points in a conductive layer on the bearing layer, and the driving electrode A, the reference electrode A, the driving electrode B and the reference electrode B are connected with an external power supply through the conductive layer. The invention adopts the closed bipolar electrode array with the symmetrical reference electrode, can effectively solve the problem that the prior closed bipolar electrode is difficult to accurately control in the processes of electrodeposition and electrochemical modification of the endpoint A array and the endpoint B array, and expands the application prospect of the bipolar electrode in the field of analysis and detection.)

1. The analysis and detection device is characterized by comprising a plurality of reaction cell A (11) arrays and reaction cell B (12) arrays which are symmetrically arranged on a bearing layer, wherein the reaction cell A (11) comprises an end point A (5) of a bipolar electrode, a driving electrode A (7) and a reference electrode A (8), and the reaction cell B (12) comprises an end point B (6) of the bipolar electrode, a driving electrode B (9) and a reference electrode B (10); the terminal A (5) and the terminal B (6) are connected with each other only through a conductive strip (13) array positioned between the two terminals in the conductive layer (2) on the bearing layer, and the driving electrode A (7), the reference electrode A (8), the driving electrode B (9) and the reference electrode B (10) are connected with an external power supply through the conductive layer (2).

2. The device for analyzing and detecting the closed bipolar electrode array with the symmetrical reference electrode according to claim 1, wherein the bearing layer is formed by overlapping a glass substrate (1), an insulating sandwich film (3) and a cover plate (4), the microwells on the cover plate (4) and the insulating sandwich film (3) form the reaction cell A (12) and the reaction cell B (11), the holes on the insulating sandwich film (3) and the conductive layer (2) on the glass substrate (1) form the end point A (5), the end point B (6), the driving electrode A (7), the reference electrode A (8), the driving electrode B (9) and the reference electrode B (10), and the end point A (5) array and the end point B (6) array are connected with the external power supply through the conductive strip (13) array.

3. The analytical test device for closed bipolar electrode arrays with symmetric reference electrodes according to claim 2, wherein: when the endpoint a (5) array is electrochemically modified, the external power source is connected in a manner that: the working electrode wiring of the external power supply is connected with the conductive strip (13) array, the reference electrode wiring of the external power supply is connected with the reference electrode A (8) array, and the counter electrode wiring of the external power supply is connected with the driving electrode A (7) array.

4. The analytical test device for closed bipolar electrode arrays with symmetric reference electrodes according to claim 2, wherein: when the endpoint B (6) array is electrochemically modified, the external power source is connected in the following manner: the working electrode wiring of the external power supply is connected with the conductive strip (13) array, the reference electrode wiring of the external power supply is connected with the reference electrode B (10) array, and the counter electrode wiring of the external power supply is connected with the driving electrode B (9) array.

5. The analytical test device for closed bipolar electrode arrays with symmetric reference electrodes according to claim 1, wherein: when the analysis detection device is used for detection, the connection mode of the external power supply is as follows: the working electrode wiring of the external power supply is connected with the driving electrode A (7) array, the reference electrode wiring of the external power supply is connected with the reference electrode B (10) array, and the counter electrode wiring of the external power supply is connected with the driving electrode B (9) array.

6. The analytical detection device of a closed bipolar electrode array with symmetric reference electrodes according to any one of claims 1 to 5, wherein: the conducting layer (2) is made of indium tin oxide, gold, platinum or a conducting carbon material.

7. The analytical detection device of a closed bipolar electrode array with symmetric reference electrodes according to any one of claims 1 to 5, wherein: the reference electrode A (8) and the reference electrode B (10) are made of Ag/AgCl, indium tin oxide, gold, platinum or conductive carbon materials.

8. The analytical detection device of a closed bipolar electrode array with symmetric reference electrodes according to any one of claims 1 to 5, wherein: the thickness of the insulating sandwich film (3) is 0.1 mm-0.5 mm.

9. The analytical test device for a closed bipolar electrode array with symmetric reference electrodes of claim 8, wherein: the insulating sandwich film (3) is made of polydimethylsiloxane or polymethyl methacrylate material.

10. The analytical detection device of a closed bipolar electrode array with symmetric reference electrodes according to any one of claims 1 to 5, wherein: the terminal A (5) and the terminal B (6) are electrodes capable of generating electrochemical, electrochemiluminescence, electrochromism or electroluminescent signals under a certain voltage, and the electrode material is ITO, Au, Pt, graphite or a surface modification electrode.

Technical Field

The invention relates to the technical field of analysis and detection, in particular to an analysis and detection device of a closed bipolar electrode array with a symmetrical reference electrode.

Background

The closed bipolar electrode is one kind of bipolar electrode, and has two physically separated ends connected via wires and two ends with oxidation-reduction reaction under certain voltage. As an analysis and detection platform, detection signals such as electrochemiluminescence, electroluminescence, electrochromism and the like generated by the signal end of the bipolar electrode are often used as detection signals to reflect the condition of the detection end of the bipolar electrode. The closed structure can effectively avoid the mutual interference of signals at two ends of the electrode, improve the sensitivity, reduce the detection limit and increase the application range. The detection device is constructed in combination with an array form, so that high-flux visual detection of multiple targets can be realized, and the application range of electrochemistry is greatly widened.

The existing closed bipolar electrode has the problem that the potential is difficult to accurately control when the electrochemical modification of the end point is carried out, and is very unfavorable for carrying out subsequent analysis and detection work. Therefore, it is desired to solve the above problems.

Disclosure of Invention

The purpose of the invention is as follows: the invention provides an analysis and detection device of a closed bipolar electrode array with a symmetric reference electrode, aiming at the problems in the prior art, and the invention adopts the closed bipolar electrode array with the symmetric reference electrode to effectively solve the problem that the prior closed bipolar electrode is difficult to accurately control in electrodeposition and electrochemical modification of an end point A array and an end point B array, thereby further expanding the application prospect of the bipolar electrode in the analysis and detection field.

The technical scheme is as follows: the invention provides an analysis and detection device of a closed bipolar electrode array with symmetrical reference electrodes, which comprises a plurality of reaction pool A arrays and reaction pool B arrays, wherein the reaction pool A arrays and the reaction pool B arrays are symmetrically arranged on a bearing layer, the reaction pool A comprises an endpoint A of a bipolar electrode, a driving electrode A and a reference electrode A, and the reaction pool B comprises an endpoint B of the bipolar electrode, a driving electrode B and a reference electrode B; the end points A and B are only connected with each other through a conductive strip array positioned between the two end points in a conductive layer on the bearing layer, and the driving electrode A, the reference electrode A, the driving electrode B and the reference electrode B are connected with an external power supply through the conductive layer.

Furthermore, the bearing layer is formed by overlapping a glass substrate, an insulating sandwich film and a cover plate, the microwell on the cover plate and the insulating sandwich film form the reaction cell A and the reaction cell B, the hole on the insulating sandwich film and the conductive layer on the glass substrate form the end point A, the end point B, the driving electrode A, the reference electrode A, the driving electrode B and the reference electrode B, and the end point A array and the end point B array are connected with the external power supply through the conductive strip array.

Further, when the endpoint a array is modified by an electrochemical method, the external power source is connected in a manner of: the working electrode wiring of the external power supply is connected with the conductive strip array, the reference electrode wiring of the external power supply is connected with the reference electrode A array, and the counter electrode wiring of the external power supply is connected with the driving electrode A array.

Further, when the endpoint B array is modified by an electrochemical method, the external power source is connected in the following manner: the working electrode wiring of the external power supply is connected with the conductive strip array, the reference electrode wiring of the external power supply is connected with the reference electrode B array, and the counter electrode wiring of the external power supply is connected with the driving electrode B array.

Further, when the analysis detection device is used for detection, the connection mode of the external power supply is as follows: the working electrode wiring of the external power supply is connected with the driving electrode A array, the reference electrode wiring of the external power supply is connected with the reference electrode B array, and the counter electrode wiring of the external power supply is connected with the driving electrode B array.

Preferably, the conductive layer is made of indium tin oxide, gold, platinum or a conductive carbon material. The conductive carbon material can be a graphite material, the shape of the conductive layer is covered with ink in a screen printing mode to form a protective layer, and then an area which is not protected by the ink is etched (the glass substrate is provided with the conductive material such as ITO glass), or the conductive layer is coated on the unprotected area in a magnetron sputtering mode (the glass substrate is not covered with the conductive layer such as Au, Pt and graphite).

Preferably, reference electrode A and reference electrode B are made of Ag/AgCl, indium tin oxide, gold, platinum, or a conductive carbon material.

Preferably, the thickness of the insulating sandwich film is 0.1 mm-0.5 mm, and the insulating sandwich film is made of polydimethylsiloxane or polymethyl methacrylate material.

Preferably, the terminal a and the terminal B are electrodes capable of generating electrochemical, electrochemiluminescence, electrochromism, or electroluminescent signals under a certain voltage, and the electrode material is ITO, Au, Pt, graphite, or a surface modified electrode.

Has the advantages that: the analysis and detection device of the closed bipolar electrode array with the symmetrical reference electrodes adopts the symmetrical reference electrode arrays at two sides and allows the symmetrical reference electrode arrays to be directly connected with an external power supply through the exposed conductive area between the end points, thereby effectively solving the problem that the prior closed bipolar electrode is difficult to accurately control when the end points are electrochemically modified, and further expanding the application prospect of the bipolar electrode in the analysis and detection field.

The analysis and detection device of the closed bipolar electrode array with the symmetrical reference electrode is a novel photoelectric analysis device, and both ends of the bipolar electrode can be subjected to operations such as electrochemical modification; by adopting the closed structure, the mutual interference between the end points A and B of the bipolar electrode can be effectively avoided, and simultaneously different detection objects can be detected simultaneously with high flux by adopting the form of an array.

Thirdly, the invention has the characteristics of low preparation cost, wide application, convenient operation and the like.

Drawings

FIG. 1 is a schematic diagram of an analytical test device with a closed bipolar electrode array with symmetrical reference electrodes;

FIG. 2 is a schematic diagram of the test of the present invention

FIG. 3 is a graph of the results of tests conducted in accordance with the present invention;

wherein, 1 is a substrate, 2 is a conductive layer, 3 is an insulating sandwich film, 4 is a cover plate, 5 is an end point A of a bipolar electrode, 6 is an end point B of the bipolar electrode, 7 is a driving electrode A, 8 is a reference electrode A, 9 is a driving electrode B, 10 is a reference electrode B, 11 is a reaction cell A, 12 is a reaction cell B, and 13 is a conductive strip.

Detailed Description

The modification of the electrochromic reagent prussian blue on the endpoint B (6) array based on a closed bipolar electrode array device in combination with electrochemical plating is further explained below with reference to the drawings.

The structure of the analysis and detection device of the closed bipolar electrode array with the symmetrical reference electrode comprises the following components:

as shown in fig. 1 and 2, the analytical detection device includes six arrays of reaction cells a11 and B12 symmetrically disposed on a carrier layer, the reaction cell a11 includes an end point A5 of a bipolar electrode, a driving electrode A7 and a reference electrode A8, and the reaction cell B12 includes an end point B6 of a bipolar electrode, a driving electrode B9 and a reference electrode B10; the terminals A5 and B6 are connected to each other only by the array of conductive strips 13 located between the terminals in the conductive layer 2 on the carrier layer, and the driving electrode A7, the reference electrode A8, the driving electrode B9 and the reference electrode B10 are connected to an external power supply through the conductive layer 2. The conductive layer 2 is preferably made of an indium tin oxide material.

The bearing layer is formed by overlapping a glass substrate 1, an insulating sandwich film 3 and a cover plate 4, a reaction pool A12 and a reaction pool B11 are formed by a micro-well on the cover plate 4 and the insulating sandwich film 3, an end point A5, an end point B6, a driving electrode A7, a reference electrode A8, a driving electrode B9 and a reference electrode B10 are formed by a hole on the insulating sandwich film 3 and a conductive layer 2 on the glass substrate 1, and an end point A5 array and an end point B6 array are connected with an external power supply through a conductive strip 13 array.

Secondly, the manufacturing method of the analysis and detection device of the closed bipolar electrode array with the symmetrical reference electrode comprises the following steps:

1.1 method for making a glass substrate 1 with a conductive layer as follows: cutting commercial ITO glass into squares with the length and width of 65mm, designing a silk screen pattern by using drawing software, processing the silk screen pattern into a silk screen, brushing ITO ink on the ITO glass by using a manual silk screen printing table, and protecting a covered area, namely a conductive area on the glass substrate 1, by the ink after drying at normal temperature; etching solution (FeCl) was used at constant temperature₃ 、HCl 、HNO₃Mixed solution of (a) and then the ink is washed away using an alkali solution to etch away the ITO not covered with the ink, and a patterned ITO glass substrate 1, here, ITO, i.e., a conductive layer 2 on the glass substrate 1, is obtained.

1.2 the method of making the PDMS cover 4 is as follows: mixing the monomers and a curing agent according to the proportion of 10:1, and curing, wherein the thickness is 5mm, and the size is 50mm x 50 mm; using a special punch (length 20mm, width 5 mm) in PDMS cover 4 with 3mm distance between the 6 parallel micro well.

1.3 preparation method of insulating sandwich film 3: a PDMS film was used as the insulating sandwich film 3, and the composition of the PDMS film was the same as the PDMS cover sheet, with a thickness of 0.2mm and a size of 50mm by 50 mm.

1.4 bonding a PDMS cover 4 and a PDMS insulating sandwich film 3 through air plasma to form 6 pairs of micro-cells which are respectively used as a reaction cell A11 array and a reaction cell B12 array, punching the PDMS sandwich film by using different punches in the micro-cells, wherein the aperture of the films for preparing an endpoint A5 and an endpoint B6 of a bipolar electrode, a driving electrode A7, a reference electrode A8, a driving electrode B9 and a reference electrode B10 is 3mm, and cutting off the cover 4 and the insulating sandwich film 3 on the conductive strip 13 array. Finally, the glass substrate 1 and the above-described structure thus prepared are bonded together by hydrophobic interaction, thereby producing a closed bipolar electrode array device, as shown in fig. 1.

And thirdly, modifying an endpoint B6 array by an electrochemical method by using the analysis and detection device of the closed bipolar electrode array with the symmetrical reference electrode:

2.1 external power supply: the working electrode wiring of the electrochemical workstation (Shanghai Hua CHI660 e) was connected to the array of conductive strips 13 using conductive tape, and the array of reference electrodes B10 and the array of drive electrodes B9 were connected to the reference electrode wiring and the counter electrode wiring of the CHI660e electrochemical workstation, respectively.

2.2 mu.l of Prussian blue plating solution was added to each array of reaction cells B12.

2.3 through a chronoamperometry method, the voltage applied to the driving electrode B9 of the bipolar electrode array by the electrochemical workstation is changed, when the voltage is set to be 0.2V (the reduction potential of the Prussian blue is 0.1V), the chronoamperometry is set to be 2000s, the Prussian blue is deposited on the endpoint B6 array in the reaction cell B12, and the effect is obvious.

Electrochemical modification on the endpoint A5 array can also be achieved in the same manner as described above.

Fourthly, the analysis and detection device of the closed bipolar electrode array with the symmetrical reference electrodes is used for detection:

3.1 an electrode A8 array was drop coated with a layer of Ag/AgCl as a reference electrode, and an end A5 array was coated with cadmium sulfide quantum dots.

3.2 external power supply: the working electrode wiring of the electrochemical workstation (shanghai hua CHI660 e) was connected to the array of drive electrodes B9 using conductive tape, and the array of reference electrodes a8 and the array of drive electrodes a7 were connected to the reference electrode wiring and the counter electrode wiring of the CHI660e electrochemical workstation, respectively.

3.3 Add 300. mu.l of ascorbic acid solution of different concentrations to each reaction well A11 array using a pipette; then 300 μ l of PBS buffer (pH =7.4, 0.1M) was added to each reaction well B12 array using a pipette.

3.4 set the potential of the electrochemical workstation to about 0.9V, fine tune the potential of the system by way of timing current, use the ultraviolet light source with wavelength of 410nm to irradiate on the endpoint A5 array coated with cadmium sulfide quantum dots, through the irradiation of 300s, can find the Prussian blue on the endpoint B6 array, turn from blue to white gradually. The degree of whitening of prussian blue from blue varies with different concentrations of ascorbic acid. The concentration of the ascorbic acid can be indirectly reflected by different degrees of color change.

The above embodiments are merely illustrative of the technical concepts and features of the present invention, and the purpose of the embodiments is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.