Self-assembled small peptide chelated zinc feed additive and preparation method thereof
阅读说明：本技术 一种自组装小肽螯合锌饲料添加剂及其制备方法 (Self-assembled small peptide chelated zinc feed additive and preparation method thereof ) 是由 山成明 邓国为 苏义雄 于 2021-09-18 设计创作，主要内容包括：本发明饲料技术领域,特别涉及一种自组装小肽螯合锌饲料添加剂及其制备方法,包括以下步骤：S1.制备小肽粉将氨基酸与强酸树脂混合在70～90℃温度下,搅拌2～5h,去除强酸树脂,干燥得到小肽粉；S2.制备小肽螯合锌将步骤S1得到的小肽粉溶解后加入无机锌溶液,在60～80℃温度下,反应0.8～2.5h；反应后浓缩,然后加入甲醇析出小肽螯合锌,纯化后即得。采用强酸树脂作为模板和催化剂,得到的小肽与无机锌螯合更加稳定。所得到的小肽螯合锌复合物在动物胃内的稳定性好,营养更加全面,可用作饲用添加剂,补充日粮的微量元素,提高动物体的免疫机能和生长速度。(The invention belongs to the technical field of feed, and particularly relates to a self-assembled small peptide chelated zinc feed additive and a preparation method thereof, wherein the preparation method comprises the following steps: s1, preparing small peptide powder, mixing amino acid and strong acid resin at the temperature of 70-90 ℃, stirring for 2-5 hours, removing the strong acid resin, and drying to obtain small peptide powder; s2, preparing small peptide chelated zinc, dissolving the small peptide powder obtained in the step S1, adding an inorganic zinc solution, and reacting at the temperature of 60-80 ℃ for 0.8-2.5 h; concentrating after reaction, adding methanol to separate out small peptide chelated zinc, and purifying to obtain the product. The strong acid resin is used as a template and a catalyst, and the obtained small peptide is chelated with inorganic zinc more stably. The obtained small peptide chelated zinc compound has good stability in the stomach of an animal and more comprehensive nutrition, can be used as a feed additive, supplements trace elements of daily ration, and improves the immune function and growth speed of the animal.)
1. A preparation method of a self-assembled small peptide chelated zinc feed additive is characterized by comprising the following steps:
s1, preparing small peptide powder
Mixing amino acid and strong acid resin at the temperature of 70-90 ℃, stirring for 2-5 h, removing the strong acid resin, and drying to obtain small peptide powder;
s2, preparing small peptide chelated zinc
Dissolving the small peptide powder obtained in the step S1, adding an inorganic zinc solution, and reacting at the temperature of 70-90 ℃ for 0.8-2.5 h; concentrating after reaction, adding methanol to separate out small peptide chelated zinc, and purifying to obtain the product.
2. The method of preparing the self-assembling small peptide chelated zinc feed additive according to claim 1, wherein the amino acid comprises one or more of glycine, alanine, valine, leucine, isoleucine, methionine, proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, aspartic acid, glutamic acid, lysine, arginine, histidine, and selenocysteine.
3. The preparation method of the self-assembly small peptide chelated zinc feed additive according to claim 1, wherein the concentration of the amino acid is 180-230 g/L.
4. The method for preparing the self-assembled small peptide chelated zinc feed additive according to claim 1, wherein the mass of the strong acid resin is 3-6% of the mass of the amino acid.
5. The preparation method of the self-assembly small peptide chelated zinc feed additive according to claim 1, wherein the inorganic zinc solution is a zinc carbonate solution with a mass fraction of 10-15%.
6. The method for preparing the self-assembly small peptide chelated zinc feed additive according to claim 1, wherein the mass ratio of the small peptide powder to the inorganic zinc added in the step S2 is (3-5): 1.
7. The method for preparing a self-assembled small peptide chelated zinc feed additive as claimed in claim 1, wherein the reaction conditions in step S1 are 80 ℃ for 3 h.
8. The method for preparing a self-assembled small peptide chelated zinc feed additive as claimed in claim 2, wherein the reaction conditions in step S2 are 80 ℃ for 2 h.
9. The small peptide chelated zinc feed additive obtained by the preparation method of the self-assembled small peptide chelated zinc feed additive as described in any one of claims 1 to 8.
Technical Field
The invention belongs to the technical field of feeds, and particularly relates to a self-assembled small peptide chelated zinc feed additive and a preparation method thereof.
Background
The animal needs specific trace elements in order to ensure the normal functions of each cell, tissue, organ, gland and system, the trace elements are indispensable nutrient substances for the animal, and the addition of certain high-quality and high-utilization rate trace elements in daily ration has very important effects on the health of the animal and the maximum exertion of the production potential of the animal.
Zinc is a necessary microelement for animal organisms, and plays an extremely important role in important physiological processes of animal growth and development, reproductive inheritance, immunity, endocrine and the like. Zinc is present in a number of enzyme systems, such as carbonic anhydrase, respiratory enzyme, lactic acid dehydro acid, superoxide dismutase, alkaline phosphatase, DNA and RNA polymerase, and the like, and is an essential substance for the synthesis of nucleic acids, proteins, carbohydrates and vitamin A utilization. The zinc has the important functions of promoting the growth and development of animals, improving the appetite of the animals, enhancing the immunity of organisms, promoting the healing of wounds and wounds, maintaining the normal sperm production function of males and the like. In the production process of livestock and poultry, zinc deficiency is also a common nutritional deficiency disease of animals, and the deficiency of zinc directly causes inappetence, growth retardation, production performance decline, abnormal reproductive function, high morbidity, depilation, chapping and other diseases.
Because the small peptide chelated zinc is a form of zinc element which is close to the natural form in the animal body, the small peptide chelated zinc has good chemical stability, higher biological value, easy digestion and absorption, interference resistance, no stimulation and no toxic action, is more superior than the corresponding organic acid state zinc and amino acid chelated zinc, and is considered as an ideal zinc supplement for feed at present. Therefore, the development of the small peptide chelated zinc with high absorption and utilization rate, good biochemical stability, high nutritional value and small side effect has very important market significance.
Disclosure of Invention
In order to overcome the problems of low bioavailability of organic trace elements and the like in the prior art, a self-assembled small peptide chelated zinc feed additive and a preparation method thereof are provided.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a preparation method of a self-assembled small peptide chelated zinc feed additive comprises the following steps:
s1, preparing small peptide powder
Mixing amino acid and strong acid resin at the temperature of 70-90 ℃, stirring for 2-5 h, removing the strong acid resin, and drying to obtain small peptide powder;
s2, preparing small peptide chelated zinc
Dissolving the small peptide powder obtained in the step S1, adding an inorganic zinc solution, and reacting at the temperature of 70-90 ℃ for 0.8-2.5 h; concentrating after reaction, adding methanol to separate out small peptide chelated zinc, and purifying to obtain the product.
Preferably, the amino acid comprises one or more of glycine, alanine, valine, leucine, isoleucine, methionine, proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, aspartic acid, glutamic acid, lysine, arginine, histidine and selenocysteine.
Preferably, the concentration of the amino acid is 180-230 g/L.
Preferably, the mass of the strong acid resin is 3-6% of the mass of the amino acid.
Preferably, the inorganic zinc solution is a zinc carbonate solution with the mass fraction of 10-15%.
Preferably, in the step S2, the mass ratio of the small peptide powder to the inorganic zinc is (3-5): 1.
Preferably, in the step S1, the reaction condition is that the reaction is carried out for 3 hours at the temperature of 80 ℃.
Preferably, in the step S2, the reaction condition is that the reaction is carried out for 2h at the temperature of 80 ℃.
The small peptide chelated zinc feed additive obtained by the preparation method of the self-assembled small peptide chelated zinc feed additive.
Compared with the prior art, the invention has the following technical effects:
the invention discloses a preparation method of a self-assembly small peptide chelated zinc feed additive, which adopts strong acid resin as a template and a catalyst, and the obtained small peptide is chelated with inorganic zinc more stably. The obtained small peptide chelated zinc compound has good stability in the stomach of an animal and more comprehensive nutrition, can be used as a feed additive, supplements trace elements of daily ration, and improves the immune function and growth speed of the animal.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The test methods used in the following experimental examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
Example 1
A preparation method of a self-assembled small peptide chelated zinc feed additive comprises the following steps:
s1, preparing small peptide powder
Mixing 230g/L amino acid and strong acid resin at 70 ℃, stirring for 5h, removing the strong acid resin, and drying to obtain small peptide powder, wherein the mass of the strong acid resin is 6% of that of the amino acid;
s2, preparing small peptide chelated zinc
Dissolving the small peptide powder obtained in the step S1, adding a zinc sulfate solution with the mass fraction of 10%, and reacting at the temperature of 80 ℃ for 0.8 h; concentrating after reaction, adding methanol to separate out small peptide chelated zinc, and purifying to obtain the small peptide chelated zinc, wherein the mass ratio of the small peptide powder to the inorganic zinc is 5: 1.
The amino acid comprises glycine, alanine, valine, leucine, isoleucine, methionine, proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, aspartic acid, glutamic acid, lysine, arginine, histidine and selenium cysteine, and is proportioned according to the requirements of the pig feed.
Example 2
A preparation method of a self-assembled small peptide chelated zinc feed additive comprises the following steps:
s1, preparing small peptide powder
Mixing amino acid with the concentration of 180g/L and strong acid resin at the temperature of 90 ℃, stirring for 2h, removing the strong acid resin, and drying to obtain small peptide powder, wherein the mass of the strong acid resin is 3% of that of the amino acid;
s2, preparing small peptide chelated zinc
Dissolving the small peptide powder obtained in the step S1, adding a zinc sulfate solution with the mass fraction of 15%, and reacting at the temperature of 60 ℃ for 2.5 h; concentrating after reaction, adding methanol to separate out small peptide chelated zinc, and purifying to obtain the small peptide chelated zinc, wherein the mass ratio of the small peptide powder to the inorganic zinc is 3: 1.
The amino acid comprises glycine, alanine, valine, leucine, isoleucine, methionine, proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, aspartic acid, glutamic acid, lysine, arginine, histidine and selenium cysteine, and is proportioned according to the requirements of the pig feed.
Example 3
A preparation method of a self-assembled small peptide chelated zinc feed additive comprises the following steps:
s1, preparing small peptide powder
Mixing 200g/L amino acid and strong acid resin at 80 ℃, stirring for 3h, removing the strong acid resin, and drying to obtain small peptide powder, wherein the mass of the strong acid resin is 3-6% of that of the amino acid;
s2, preparing small peptide chelated zinc
Dissolving the small peptide powder obtained in the step S1, adding a zinc sulfate solution with the mass fraction of 12%, and reacting for 2 hours at the temperature of 80 ℃; concentrating after reaction, adding methanol to separate out small peptide chelated zinc, and purifying to obtain the small peptide chelated zinc, wherein the mass ratio of the small peptide powder to the inorganic zinc is 4: 1.
The amino acid comprises glycine, alanine, valine, leucine, isoleucine, methionine, proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, aspartic acid, glutamic acid, lysine, arginine, histidine and selenium cysteine, and is proportioned according to the requirements of the pig feed.
Examples of the experiments
The basic diet is prepared according to NRC (2012) pig nutrition requirements in the pig feeding Standard (NY/T65-2004) of China, the basic diet adopts a corn-soybean meal type, and a control group adopts inorganic trace elements (blue vitriol) as a nutrition supplement; the test group added the small peptide chelated copper obtained in example 3 to replace equivalent copper sulfate pentahydrate, and the basic feed composition and various nutritional indexes are shown in the following table:
item
Control group
Test group
Raw materials (air drying foundation)
Corn (corn)
63.45
63.58
Bean pulp (CP 46%)
17.68
17.60
Bran
4.3
4.5
Wheat middling
1.2
1.1
Fish meal
2.5
2.5
Lysine (98%)
0.42
0.42
Methionine (99%)
0.1
0.1
Threonine (98.5%)
0.09
0.09
Soybean oil
1.5
1.5
Premix (Dry peptide powder + copper sulfate)
4.00
-
Premix (Small peptide chelated copper)
-
4.00
Total up to
100
100
Nutrition level (Dry matter basis)
Crude protein
18.11
18.42
Lysine
1.01
1.03
Methionine
0.71
0.73
Threonine
0.87
0.76
Calcium carbonate
0.70
0.72
Total phosphorus
0.65
0.67
Available phosphorus
0.35
0.37
Crude fat
8.20
8.50
Digestion energy (MJ/kg)
16.23
18.30
The premix is provided for each kilogram of feed: 50mg of zinc sulfate monohydrate/small chelated zinc, 20mg of copper sulfate pentahydrate, 500mg of ferrous sulfate heptahydrate, 10mg of magnesium sulfate, 0.2mg of potassium iodide, 20mg of manganese sulfate monohydrate, VA 5600IU, VD 3180 IU, VB 19mg, 17mg of citric acid, 3mg of antioxidant, 5mg of chromium picolinate, 250mg of calcium dihydrogen phosphate, 300mg of mountain flour and zeolite powder as a carrier.
The nutrient levels are calculated values.
A single-factor test is adopted to involve that 80 healthy piglets with similar weights in 45 +/-1 days are selected and randomly divided into 2 groups, each group is 8 in number of repetitions, each piglet is 10 in number of repetitions, and the 2 kinds of feed are respectively fed to the piglets, and the test period is 75 days. The grouping of the experimental piglets is as follows:
group of
Additive for different zinc sources in daily ration
Control group
Small peptide powder and zinc sulfate monohydrate
Test group
Small peptide chelated zinc
Measurement indexes are as follows: initial average weight, final average weight, average daily feed intake, average daily gain, material-to-weight ratio, survival rate, the results are shown in the following table:
item
Control group
Test group
Initial weight average/kg
10.27±0.32
10.89±0.27
End average weight/kg
23.08±0.80
34.58±0.54
Average daily food intake/(kg/d)
0.59±0.05
0.63±0.02
Average daily gain/(kg/d)
0.37±0.04
0.41±0.05
Material to weight ratio
1.75±0.08
1.70±0.03
Diarrhea Rate (%)
5..13±0.61
4.22±0.22
From the table above, it can be seen that the growth speed of the piglets in the experimental group is higher, and the feed consumption is less, which indicates that the feed of the invention is more beneficial to the digestion and absorption of the piglets, and improves the immune function and the growth speed of the animals.
The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.