Non-human primate animal behavioral interactive training inducer, preparation method and application method thereof
阅读说明：本技术 一种非人灵长类动物行为学互动训练诱导剂、制备方法及其应用方法 (Non-human primate animal behavioral interactive training inducer, preparation method and application method thereof ) 是由 孙国栋 刘晓文 沃金 周立兵 李志忠 于 2021-09-30 设计创作，主要内容包括：本发明公开一种非人类灵长类动物行为学互动训练诱导剂、制备方法及其应用方法。本发明所述的非人类灵长类动物行为学互动训练诱导剂按其重量份包括以下成分：麦芽糊精15-25重量份；食用糖霜15-25重量份；蔗糖或木糖醇20重量份；山楂粉20重量份；奶粉10-20重量份；低聚果糖粉0-10重量份。本发明所制得灵长类动物行为学互动训练诱导剂,添加在猕猴的行为学实验中饲料中,可以明显提升动物行为学数据采集持续时间。猕猴在手术后,恢复期间,添加本发明所述的诱导剂可使非人灵长类动物术后立刻进食,有助于动物术后的康复,并且食物过量食用后不会使动物产生食物厌倦。(The invention discloses a non-human primate behavioristics interactive training inducer, a preparation method and an application method thereof. The non-human primate behavioral interactive training inducer comprises the following components in parts by weight: 15-25 parts of maltodextrin; 15-25 parts of edible sugar cream; 20 parts of sucrose or xylitol; 20 parts of hawthorn powder; 10-20 parts of milk powder; 0-10 parts of fructo-oligosaccharide powder. The primate behavioural interactive training inducer prepared by the invention is added into feed in behavioural experiments of macaques, so that the animal behavioural data acquisition duration can be obviously prolonged. After the operation and in the recovery period of the macaque, the non-human primate can eat immediately after the operation by adding the inducer, the postoperative rehabilitation of the macaque is facilitated, and the animals cannot be tired of food after the excessive food is eaten.)
1. A non-human primate animal behavioral interactive training inducer is characterized by comprising the following components in parts by weight:
2. the non-human primate behavioral training inducer according to claim 1, characterized by comprising the following components in parts by weight:
3. the non-human primate behavioural interactive training inducer of claim 2, wherein:
the edible sugar cream is a mixture of cooked corn starch and sucrose powder, and the weight ratio of the cooked corn starch to the sucrose powder is (0.7-1): (0.7-1).
4. The non-human primate behavioural interactive training inducer of claim 3, wherein:
the edible sugar cream is a mixture of cooked corn starch and sucrose powder, and the weight ratio of the cooked corn starch to the sucrose powder is 0.85: 0.85.
5. The non-human primate behavioural interactive training inducer of claim 4, wherein:
the maltodextrin, edible sugar cream, sucrose, xylitol, hawthorn powder, milk powder and fructo-oligosaccharide powder are all food grade.
6. A method for preparing the non-human primate behavioral interactive training inducer according to any one of claims 1-5, characterized in that:
mixing maltodextrin, edible sugar cream, sucrose or xylitol, fructus crataegi powder and milk powder at a certain proportion, and making into inducer.
7. An application method for preparing the non-human primate animal behavioral interactive training inducer as claimed in any one of claims 1 to 5, wherein the inducer is added into the feed of a primate animal, and the weight ratio of the inducer to the feed is 3: 10.
Technical Field
The invention belongs to the field of animal feed, and relates to a primate behavioristics interactive training inducer, and a preparation method and an application method thereof.
Background
Animal experiments are often required to gain new knowledge about biology, medicine, etc. or to solve specific problems. The animals used in the animal experiments are usually mice, rats, guinea pigs, hamster, rabbit, dog and monkey, experimental miniature pigs and macaques, etc.
The non-human primate used in the present experiment generally refers to a macaque (Rhesus monkey; Macaca multata zimmermann) belonging to the mammalia, primates, monkeys, macaque. The macaque is a general term of macaques, has 12 species in total, is a non-human primate animal, is a close animal of human, is similar to human in the aspects of tissue structure, physiology, metabolic function and the like, is most easy to solve human similar diseases and pathogenesis, and some human diseases can be successfully treated only by using the macaque as an experiment and cannot be replaced by other experimental animals. For example, modeling of spinal cord injury experiments, which have been performed with rodents (mice), is generally performed, but rodents are too different from humans in neural structure and spinal cord immune function, and therefore, non-human primate experiments are required to fill the gap. The behavioral testing of the non-human primate is an important evaluation index after the spinal cord injury experiment modeling.
When the ethology of the non-human primates is tested, common feed or sugar is single in taste, and after the ethology is rewarded for a long time, the duration of the collection of ethological effective data is gradually reduced, so that the long-time test cannot be maintained. In addition, since the use of a large amount of anesthetic during animal experimental surgery reduces the physical functions of non-human primates, including appetite, mental state, etc., there is a need for food to promote recovery of appetite after surgery.
Disclosure of Invention
The invention aims to provide a primate behavioristics interactive training inducer which is added into feed of macaques in animal experiments, is helpful for animals to eat and helps postoperative recovery. In addition, the inducer can reward the non-human primates in the behavioural detection process, improve the degree of matching in the behavioural detection process of the non-human primates, and prolong the time for collecting effective data.
It is necessary to provide a method for preparing the above inducer.
Further, there is a need to provide methods for the use of the above-mentioned inducers.
A non-human primate animal behavioral interactive training inducer comprises the following components in parts by weight:
15-25 parts of maltodextrin;
15-25 parts of edible sugar cream;
20 parts of sucrose or xylitol;
20 parts of hawthorn powder;
10-20 parts of milk powder;
0-10 parts of fructo-oligosaccharide powder.
Preferably, the non-human primate animal behavioral interaction training inducer comprises the following components in parts by weight:
20 parts of maltodextrin;
20 parts of edible sugar cream;
20 parts of sucrose or xylitol;
20 parts of hawthorn powder;
15 parts of milk powder;
5 parts of fructo-oligosaccharide powder.
Wherein the edible sugar cream is a mixture of cooked corn starch and sucrose powder, and the weight ratio of the cooked corn starch to the sucrose powder is (0.7-1): (0.7-1).
Preferably, the edible sugar cream is a mixture of cooked corn starch and sucrose powder, and the weight ratio of the cooked corn starch to the sucrose powder is 0.85: 0.85.
Wherein the maltodextrin, edible sugar cream, sucrose, xylitol, hawthorn powder, milk powder and fructo-oligosaccharide powder are all food grade.
A method for preparing the non-human primate animal behavioral interactive training inducer,
mixing maltodextrin, edible sugar cream, sucrose or xylitol, fructus crataegi powder and milk powder at a certain proportion, and making into inducer.
The application method for preparing the non-human primate animal behavioral interactive training inducer comprises the following steps of adding the inducer into feed of a primate animal, wherein the weight ratio of the inducer to the feed is 3: 10.
the fructo-oligosaccharide powder is fructo-oligosaccharide.
The primate behavioural interactive training inducer prepared by the invention is added into feed in behavioural experiments of macaques, so that the animal behavioural data acquisition duration can be obviously prolonged. After the operation and in the recovery period of the macaque, the non-human primate can eat immediately after the operation by adding the inducer, the postoperative rehabilitation of the macaque is facilitated, and the animals cannot be tired of food after the excessive food is eaten.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments in order to make the technical field better understand the scheme of the present invention.
Description of the raw materials
Model number
Manufacturer(s)
Maltodextrin
Food grade maltodextrin
No particular manufacturer (Tianmao 'mountain family flagship shop')
Sugar cream
Taigu 1Edible sugar cream
Chengdu Taigu sugar industry Co Ltd
Fructo-oligosaccharide powder (fructo-oligosaccharide)
Food grade
No particular manufacturer (Tianmao 'mountain family flagship shop')
Sucrose
Taigu white granulated sugar
Chengdu Taigu sugar industry Co Ltd
Xylitol, its preparation method and use
Taigu xylitol
Chengdu Taigu sugar industry Co Ltd
Hawthorn powder
Food-grade denucleated superfine hawthorn powder
High-grade seedless hawthorn powder without specific merchant
Milk powder
Lamb milk powder for animals
Normal milk replacer without specific trade company
Example 1
Mixing cooked corn starch and sucrose powder in a ratio of 0.7: 1 to obtain edible sugar cream, and then evenly mixing maltodextrin, the edible sugar cream, cane sugar or xylitol, hawthorn powder, milk powder and fructo-oligosaccharide powder (fructo-oligosaccharide) according to the weight parts shown in the table 1 to obtain the inducer.
Example 2
Mixing cooked corn starch and sucrose powder at a ratio of 0.85:0.85 weight ratio to obtain edible sugar cream, and then evenly mixing maltodextrin, the edible sugar cream, cane sugar or xylitol, hawthorn powder, milk powder and fructo-oligosaccharide powder (fructo-oligosaccharide) according to the weight parts shown in the table 1 to obtain the inducer.
Example 3
Mixing cooked corn starch and sucrose powder in a ratio of 1: 0.7 weight ratio to obtain edible sugar cream, and then evenly mixing maltodextrin, the edible sugar cream, cane sugar or xylitol, hawthorn powder, milk powder and fructo-oligosaccharide powder (fructo-oligosaccharide) according to the weight parts shown in the table 1 to obtain the inducer.
Comparative example 1
Mixing cooked corn starch and sucrose powder at a ratio of 0.85:0.85 weight ratio to obtain edible sugar cream, and then evenly mixing maltodextrin, the edible sugar cream, cane sugar or xylitol, hawthorn powder, milk powder and fructo-oligosaccharide powder (fructo-oligosaccharide) according to the weight parts shown in the table 1 to obtain the inducer.
Comparative example 2
Mixing cooked corn starch and sucrose powder at a ratio of 0.85:0.85 weight ratio to obtain edible sugar cream, and then evenly mixing maltodextrin, the edible sugar cream, cane sugar or xylitol, hawthorn powder, milk powder and fructo-oligosaccharide powder (fructo-oligosaccharide) according to the weight parts shown in the table 1 to obtain the inducer.
Comparative example 3
Mixing cooked corn starch and sucrose powder at a ratio of 0.85:0.85 weight ratio to obtain edible sugar cream, and then evenly mixing maltodextrin, the edible sugar cream, cane sugar or xylitol, hawthorn powder, milk powder and fructo-oligosaccharide powder (fructo-oligosaccharide) according to the weight parts shown in the table 1 to obtain the inducer.
Comparative example 4
Mixing cooked corn starch and sucrose powder at a ratio of 0.85:0.85 weight ratio to obtain edible sugar cream, and then evenly mixing maltodextrin, the edible sugar cream, cane sugar or xylitol, hawthorn powder, milk powder and fructo-oligosaccharide powder (fructo-oligosaccharide) according to the weight parts shown in the table 1 to obtain the inducer.
Comparative example 5
Mixing cooked corn starch and sucrose powder at a ratio of 0.85:0.85 weight ratio to obtain edible sugar cream, and then evenly mixing maltodextrin, the edible sugar cream, cane sugar or xylitol, hawthorn powder, milk powder and fructo-oligosaccharide powder (fructo-oligosaccharide) according to the weight parts shown in the table 1 to obtain the inducer.
TABLE 1 materials used in examples 1-3 and comparative examples 1-5
Example 1
Example 2
Example 3
Comparative example 1
Comparative example 2
Comparative example 3
Comparative example 4
Comparative example 5
Maltodextrin
15
20
25
0
20
20
20
20
Edible sugar cream
25
20
15
20
0
20
20
20
Sucrose
20
20
0
20
20
0
20
20
Xylitol, its preparation method and use
0
0
20
0
0
0
0
0
Hawthorn powder
20
20
20
20
20
20
0
20
Milk powder
10
15
20
15
15
15
15
0
Fructo-oligosaccharide powder
10
5
0
5
5
5
5
5
Method of implementation
The inducers prepared in examples 1 to 3 and comparative examples 1 to 5 were added to the feed of macaques to prepare feed tablets with a weight of 1g, respectively. The feed used in the present embodiment was a feed for experimental monkeys (product model HWC9201) of cooperative pharmaceutical and bioengineering, llc of jiangsu province, and the amount of the inducer added was 30 wt% (the weight ratio of the inducer to the feed was 3: 10).
Animal number: 27 macaques are divided into 9 groups, after the animals are subjected to spinal cord injury modeling operation, feeding the macaques after each group of operations within 6 hours, feeding the macaques in an inducer group, feeding the macaques in a control group without the inducer, and recording the average food intake and the interval time of food intake of each macaque. In addition, at one week after the postoperative rehabilitation of two groups of macaques, the animal was subjected to the behavioral testing, and the duration of animal behavioral data collection and the frequency of food boredom were recorded in the test process by administering the ordinary feed and the feeds to which the inducers prepared in examples 1 to 3 and comparative examples 1 to 5 were added, respectively, in groups, and are shown in table 2.
Average food intake: during the experiment, each animal in each group was given 3min of behavioural training and was rewarded with 5 feed tablets of 1g weight. The behavioral tests were performed 10 times in total and the weight of the animals consumed food was recorded, i.e. the average food intake was calculated.
Animal behavioural data acquisition duration: the ethological collection lasts for 3min each time, and 10 times total collection is performed. The acquisition process of the behavioral gait data is completed by at least four stereo cameras in cooperation with an analysis terminal provided with behavioral gait analysis related software Motion Recorder software, and the experimental animals are marked on multiple joints of the body including the bilateral iliac crest, hip joint, knee joint, ankle joint, toe joint, shoulder joint, elbow joint and wrist joint by fluorescent paint spraying before data acquisition; then, the experimental animal walks on an animal movement device of a behavioural gait analyzer at the speed of 3km/h, four three-dimensional cameras acquire animal gait data, the image pickup frequency is 60Hz, the acquired gait data are transmitted to an analysis terminal, the gait data are filtered and automatically identified and processed through 3 DCalgorithm software in the analysis terminal, the real-time 3D coordinates of each fluorescent marker are acquired, and the acquired three-dimensional model data are converted into a rod-shaped three-dimensional model. The gait meter capable of being automatically identified by the analysis terminal can be used for making standard gait, timing in seconds and counting as the duration time of behavioural data acquisition.
Frequency of food boredom: the 5g feed tablets were administered 10 times consecutively and the number of times in which the animals were not fed or boredom fed was recorded (boredom fed is recorded as 5g of food not consumed each time).
TABLE 2 feed effect of general feed, feed supplemented with examples and comparative examples
As can be seen from the data in Table 2, the feed added in the examples 1 to 3 is obviously more popular with primates, the frequency of food boredom is obviously reduced, the duration of animal behavior data acquisition is longer, and the animal experiment is more favorably carried out.
The above-described embodiments are merely preferred embodiments of the present invention, and it should be noted that, for those skilled in the art, several modifications can be made without departing from the principle of the present invention, and these modifications should also be construed as the protection scope of the present invention.