Compound for nucleic acid liquid phase synthesis carrier and preparation method and application thereof

文档序号：400799 发布日期：2021-12-17 浏览：16次中文

阅读说明：本技术 一种用于核酸液相合成载体的化合物及其制备方法和用途 (Compound for nucleic acid liquid phase synthesis carrier and preparation method and application thereof ) 是由李进陈兴王星秦志奇范国盛万金桥于 2021-06-15 设计创作，主要内容包括：本发明涉及一种用于核酸液相合成载体的化合物及其制备方法和用途,具体公开了一种可以作为核酸液相合成载体的稠环化合物,其具有如下式I所示,其中,X选自O、NH、-(CH-(2))-(n)O-或化学键,Y选自CH或N,为单键或者双键；m的取值为1或2；n的取值为1或2。本发明的液相合成载体化合物制备简单,反应效率高,可重复利用。(The invention relates to a compound for a nucleic acid liquid phase synthesis carrier, and a preparation method and application thereof, and particularly discloses a fused ring compound capable of being used as a nucleic acid liquid phase synthesis carrier, which is shown as the following formula I, wherein X is selected from O, NH, - (CH) 2 ) n O-or a chemical bond, Y is selected from CH or N, is a single bond or a double bond; m takes the value of 1 or 2; n takes the value of 1 or 2. The liquid-phase synthesis carrier compound is simple to prepare, high in reaction efficiency and capable of being repeatedly used.)

1. A compound for use in nucleic acid liquid phase synthesis of a carrier, characterized by:

wherein X is selected from O, NH, - (CH)₂)_nO-or a chemical bond, Y is selected from CH or N,is a single bond or a double bond; m takes the value of 1 or 2; n takes the value of 1 or 2;

R₂、R₃each independently selected from hydrogen or C₁～C₁₀Or R is₂、R₃Connecting to form a 5-to 8-membered aromatic ring; and/or the 5-to 8-membered aromatic ring is substituted by 0 to 5R₄Substitution; r₁0 to 4;

each R₁、R₄Each independently selected from hydrogen OR-OR₅Said R is₅Is selected from C₁～C₁₀₀Alkyl radical, C₂～C₁₀₀Alkenyl radical, C₂～C₁₀₀Alkynyl, C₃～C₁₀₀Cycloalkyl radical, C₁～C₁₀₀Alkyl substituted C₃～C₁₀₀Cycloalkyl radical, C₁～C₅₀Alkoxy-substituted C₃～C₁₀₀Cycloalkyl, substituted C₁～C₁₀₀An alkyl group;

substituted C₁～C₁₀₀The number of substituents of the alkyl group is one or more; the substituents of the substituted alkyl groups being independently of one another selected from C₁～C₅₀Alkoxy radical, C₃～C₁₀₀Cycloalkyl radical, C₁～C₅₀Alkoxy-substituted C₃～C₁₀₀Cycloalkyl, 5-to 8-membered aryl, C₁～C₅₀Alkoxy-substituted 5-to 8-membered aryl.

2. The compound of claim 1, wherein: the compound of the formula I is:

wherein X is selected from O, NH, - (CH)₂)_nO-or a chemical bond; y is selected from CH or N; n takes the value of 1 or 2; r₁、R₄0-4 respectively;

3. The compound of claim 2, wherein: the compound of the formula I is:

4. the compound of claim 1, wherein: the compound of the formula I is:

wherein X is selected from O, NH or- (CH)₂)_nO-; m takes the value of 1 or 2;n is 1 or 2, and Y is selected from CH or N; r₁0 to 4;

each R₁Each independently selected from hydrogen OR-OR₅Said R is₅Is selected from C₁～C₁₀₀Alkyl radical, C₂～C₁₀₀Alkenyl radical, C₂～C₁₀₀Alkynyl, C₃～C₁₀₀Cycloalkyl radical, C₁～C₁₀₀Alkyl substituted C₃～C₁₀₀Cycloalkyl radical, C₁～C₅₀Alkoxy-substituted C₃～C₁₀₀Cycloalkyl, substituted C₁～C₁₀₀An alkyl group;

5. The compound of claim 4, wherein: the compound of the formula I is:

6. the compound of claim 1, wherein: the compound of the formula I is:

x is selected from O, NH or- (CH)₂)_nO-; n is 1 or 2, and Y is selected from CH or N; r₁0 to 4;

R₁each independently selected from hydrogen OR-OR₅Said R is₅Is selected from C₁～C₁₀₀Alkyl radical, C₂～C₁₀₀Alkenyl radical, C₂～C₁₀₀Alkynyl, C₃～C₁₀₀Cycloalkyl radical, C₁～C₁₀₀Alkyl substituted C₃～C₁₀₀Cycloalkyl radical, C₁～C₅₀Alkoxy-substituted C₃～C₁₀₀Cycloalkyl, substituted C₁～C₁₀₀An alkyl group;

7. The compound of claim 6, wherein: the compound of the formula I is:

8. use of a compound according to claim 1 as a support for liquid phase synthesis.

9. Use of the compound of claim 1 for the liquid phase synthesis of nucleic acids.

10. A method for synthesizing nucleic acid, which comprises using the compound as shown in claim 1 as a liquid phase synthesis carrier, and sequentially coupling different nucleoside monomer fragments on the functional groups of the compound as shown in formula I.

Technical Field

The invention belongs to the technical field of nucleic acid synthesis, and particularly relates to a compound for a nucleic acid liquid phase synthesis carrier, and a preparation method and application thereof.

Background

Chemically synthesized oligonucleotides refer to the process of joining multiple nucleotide units into an oligonucleotide chain by promoting the formation of 5 '-3' phosphodiester bonds between nucleotide monomers, involving the synthesis of protected nucleotides. The main synthesis method comprises the following steps: a phosphotriester process, an H-phosphonate process, a phosphoramidite process, and the like.

The current general oligonucleotide synthesis method is a solid phase synthesis method using the phosphoramidite method, in which the 5 '-OH on the nucleotide is first protected with di-p-methoxytrityl (DMT), the amino group on the base is protected with benzoyl, and the 3' -OH is activated with an amino phosphite compound. Combining the 3' -OH of the first nucleotide with the solid phase resin, and removing the protecting group on the 5' -OH to form a phosphite triester between the exposed 5' -OH and the 3' -OH of the second nucleotide activated with the amino phosphite compound, oxidizing the phosphite triester with iodine to form a phosphate triester, and adding trichloroacetic acid to remove the protecting group on the 5' -OH of the second nucleotide. To this end, the oligonucleotide strand has been extended by one nucleotide unit and can be put into the next round of extension reaction. After several rounds of extension reactions, the oligonucleotide fragment is eluted from the solid phase resin with concentrated ammonium hydroxide after the synthesis of the whole oligonucleotide fragment, and the oligonucleotide is obtained through deprotection and purification.

The solid phase method is advantageous in view of speed, since the method has been optimized and automation has been developed. However, it has the disadvantages that: scale-up is limited due to equipment limitations, excess reagents and starting materials are used, and determination of the state of reaction progress in intermediate steps, analysis of intermediate structures, and the like are difficult.

In order to improve the defects of the traditional solid-phase synthesis method in recent years, scientists invent a method of combining liquid-phase synthesis and liquid-phase carriers for nucleotide compound synthesis, and the liquid-phase carriers with specific structures are designed, nucleotide coupling is carried out in the liquid-phase synthesis, and nucleic acid synthesis is carried out by combining the purification and post-treatment methods of solid-phase synthesis and liquid-phase synthesis intermediates.

Disclosure of Invention

In order to improve the disadvantages of the solid phase synthesis method, the invention discloses a condensed ring compound which can be used as a nucleic acid liquid phase carrier and has the following formula (I):

wherein X is selected from O, NH, - (CH)₂)_nO-or a chemical bond, Y is selected from CH or N,is a single bond or a double bond; m takes the value of 1 or 2; n takes the value of 1 or 2;

R₁、R₄each independently selected from hydrogen OR-OR₅Said R is₅Is selected from C₁～C₁₀₀Alkyl radical, C₂～C₁₀₀Alkenyl radical, C₂～C₁₀₀Alkynyl, C₃～C₁₀₀Cycloalkyl radical, C₁～C₁₀₀Alkyl substituted C₃～C₁₀₀Cycloalkyl radical, C₁～C₅₀Alkoxy-substituted C₃～C₁₀₀Cycloalkyl, substituted C₁～C₁₀₀An alkyl group;

As preferred; the compound of the formula I is:

wherein X is selected from O, NH or- (CH)₂)_nO-or a chemical bond; y is selected from CH or N; n is 1 or 2, R₁、R₄0-4 respectively;

substituted C₁～C₁₀₀The number of substituents of the alkyl group is one or more; substituted alkanesThe substituents of the radicals being independently of one another selected from C₁～C₅₀Alkoxy radical, C₃～C₁₀₀Cycloalkyl radical, C₁～C₅₀Alkoxy-substituted C₃～C₁₀₀Cycloalkyl, 5-to 8-membered aryl, C₁～C₅₀Alkoxy-substituted 5-to 8-membered aryl.

Further, the compound of formula I is:

preferably, the compound of formula I is:

wherein X is selected from O, NH or- (CH)₂)_nO-; m takes the value of 1 or 2; n is 1 or 2, and Y is selected from CH or N; r₁0 to 4;

Further, the compound of formula I is:

preferably, the compound of formula I is:

x is selected from O, NH or- (CH)₂)_nO-; n is 1 or 2, and Y is selected from CH or N; r₁0 to 4;

Further, the compound of formula I is:

in another aspect, the invention provides the use of a fused ring compound of formula 1 as a support for liquid phase synthesis.

In another aspect, the invention provides the use of the fused cyclic compound of formula 1 for the liquid phase synthesis of nucleic acids.

In still another aspect, the present invention provides a method for synthesizing a nucleic acid, which comprises using the compound of claim 1 as a liquid phase synthesis support, and sequentially coupling different nucleosidic monomer fragments to the functional groups of the compound of formula I.

The invention provides a nucleic acid synthesis method, which takes a compound shown in formula I as a liquid phase synthesis carrier, takes a coupling precursor of a nucleoside or nucleoside polymer protected by 5 '-DMTr (4,4' -dimethoxytriphenylmethyl) on a functional group of the compound shown in formula I as a starting material, carries out deprotection and coupling with a nucleic acid monomer or a polymer (such as dimer and trimer) compound to produce a nucleic acid polymer, or carries out oxidation or thio reaction on the nucleic acid polymer, then further repeats similar circulation until the synthesis of the final nucleic acid is finished, and finally carries out excision of the liquid phase synthesis carrier and removal of a protecting group to obtain a corresponding target nucleic acid polymer compound or salt.

Has the advantages that:

1. the liquid phase carrier prepared by the invention is used for nucleic acid synthesis, has simple and convenient operation compared with the traditional liquid phase synthesis, does not need special reaction equipment, such as: DNA synthesizer, etc.;

2. the liquid phase carrier prepared by the invention is used for nucleic acid synthesis, and the synthesis scale is larger than that of the traditional solid phase synthesis;

3. the liquid phase carrier prepared by the invention is used for nucleic acid synthesis, has better reaction effect, is easy to recover and can be repeatedly used;

4. the liquid phase carrier prepared by the invention is used for nucleic acid synthesis, can effectively reduce impurities and improve the quality of nucleic acid products;

5. the liquid phase carrier prepared by the invention is used for nucleic acid synthesis, can greatly reduce the use of monomer materials, reagents and solvents, has the cost advantage compared with solid phase synthesis, generates less waste and has more green and environment-friendly process.

Definitions of terms used in connection with the present invention: the initial definitions provided herein for a group or term apply to that group or term throughout the specification unless otherwise indicated; for terms not specifically defined herein, the meanings that would be given to them by a person skilled in the art are to be given in light of the disclosure and the context.

"substituted" means that a hydrogen atom in a molecule is replaced by a different atom or molecule.

The minimum and maximum values of the carbon atom content in the hydrocarbon group are indicated by a prefix, e.g. prefix (C)_a～C_b) Alkyl means any alkyl group containing from "a" to "b" carbon atoms. Thus, for example, C₁～C₂₀The alkyl group is a straight-chain or branched alkyl group having 1 to 20 carbon atoms.

Alkyl means a straight or branched hydrocarbon radical in an alkane molecule, e.g. methyl-CH₃ethyl-CH₂CH₃methylene-CH₂-; the alkyl group may also be part of another group, such as C₁～C₆Alkoxy radical, C₁～C₆An alkylamino group.

Alkoxy groups: means that the alkyl radical is bound to an oxygen atom to form a substituent, e.g. methoxy is-OCH₃。

Cycloalkyl groups: refers to saturated or partially saturated cyclic groups having multiple carbon atoms and no ring heteroatoms and having a single ring or multiple rings (including fused, bridged, and spiro ring systems).

5-to 8-membered aromatic ring/radical: refers to an aromatic single ring or multiple cyclic groups composed of C atoms and containing no hetero atoms.

Alkenyl: including straight or branched alkenyl groups.

Alkynyl: including straight or branched alkynyl groups.

Obviously, many modifications, substitutions, and variations are possible in light of the above teachings of the invention, without departing from the basic technical spirit of the invention, as defined by the following claims.

The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.

Detailed Description

The raw materials and equipment used in the invention are known products and are obtained by purchasing commercial products.

In the invention, the term "room temperature" means 20-25 ℃.

The embodiment of the invention adopts the following characters of shorthand or English: DMF: n, N-dimethylformamide; DMAP: 4-dimethylaminopyridine; DCM: dichloromethane; cholesterol: cholesterol; the DIAD: diisopropyl azodicarboxylate; THF: tetrahydrofuran; 2-Bromoethane: bromoethanol; 1-Bromooctadecanoe: 1-bromooctadecane; succinic anhydride: succinic anhydride.

The term "mass% as used herein means mass%.

Example 1 Synthesis of Compound 1

Step 1: synthesis of Compound 1b

Dissolve 1a (7.0g,35.7mmol) in N, N-dimethylformamide (140.0mL) at room temperature, add potassium carbonate (9.8g,71.4mmol), 1-bromooctadecane (23.7g,71.4mmol), stir the reaction mixture at 80 ℃ for 15 hours, add water to the reaction mixture, extract with dichloromethane, dry concentrate to give crude product, add methanol to the crude product and filter to give 1b (11.2g, 24.5mmol) as a white solid in 70% yield.¹H-NMR(400MHz,CDCl₃)δ＝8.44(d,J＝4.0Hz,1H),8.34(d,J＝4.0Hz,1H),7.78-7.24(m,5H),4.06(m,2H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝448.4。

Step 2: synthesis of Compound 1c

Dissolving 1b (11.2g, 24.5mmol) in methanol (112.0mL) at room temperature, adding sodium borohydride (1.4g,36.7mmol) under ice bath, stirring the reaction mixture at 0 ℃ for 0.5 h, adding saturated aqueous ammonium chloride solution to the reaction solution, extracting with dichloromethane, drying and concentrating to obtain a crude product, adding methanol to the crude product, and filtering to obtain 1c (9.9g, 22.05mmol) white solid with 90% yield。¹H-NMR(400MHz,CDCl₃)δ＝8.44(d,J＝4.0Hz,1H),8.34(d,J＝4.0Hz,1H),7.78-7.24(m,5H),6.43(s,1H),5.60(s,1H),4.06(m,2H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝450.4。

And step 3: synthesis of Compound 1

1c (9.9g, 22.05mmol) was dissolved in anhydrous dichloromethane (100.0mL) at room temperature, triethylamine (5.5g,55.1mmol), 4-dimethylaminopyridine (6.7g,55.1mmol), succinic anhydride (3.3g,33.1mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, drying was concentrated to give a crude product, the crude product was filtered by adding methanol to give 1(10.3g, 18.7mmol) as a white solid in 85% yield.¹H-NMR(400MHz,CDCl₃)δ＝11.01(s,1H),8.44(d,J＝4.0Hz,1H),8.34(d,J＝4.0Hz,1H),7.78-7.24(m,5H),6.43(s,1H),5.60(s,1H),4.06(m,2H),2.83-2.52(m,4H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝551.3。

By following the same procedure as above, with other conditions being maintained, different alkyl bromides are added in step 1 to obtain different R-substituted compounds 1. R is-OC₁₈H₃₃,-OC₁₉H₃₉,-OC₂₇H₄₅,-OC₃₇H₆₇,-OC₅₉H₁₁₉,-OC₆₁H₁₁₅,-OC₆₁H₁₂₁。

Example 2 Synthesis of Compound 2

Step 1: synthesis of Compound 2c

Dissolve 2b (5.0g,11.1mmol) in methanol (100.0mL) at room temperature, add ammonium formate (7.0g,111mmol), 10% palladium on carbon (700mg), stir the reaction mixture at room temperature under nitrogen for 15 hours, filter the reaction and wash with dichloromethane 3 times, concentrate the mother liquor to give crude product, add methanol to the crude product and filter to give 2c (2.99g, 6.66mmol) as a white solid in 60% yield.¹H-NMR(400MHz,CDCl₃)δ＝8.44(d,J＝4.0Hz,1H),8.34(d,J＝4.0Hz,1H),7.78-7.24(m,5H),5.11(s,1H),5.01(s,1H),4.06(m,2H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝450.4。

Step 2: synthesis of Compound 2

Dissolve 2c (2.99g, 6.66mmol) in anhydrous dichloromethane (50.0mL) at room temperature, add triethylamine (1.3g,13.3mmol), 4-dimethylaminopyridine (1.6g,13.3mmol), succinic anhydride (9.9g,9.99mmol), stir the reaction mixture at room temperature for 15 hours, add water to the reaction mixture, extract with dichloromethane, dry and concentrate to give a crude product, add methanol to the crude product and filter to give 2(2.9g, 5.3mmol) as a white solid in 80% yield.¹H-NMR(400MHz,CDCl₃)δ＝11.12(s,1H),8.44(d,J＝4.0Hz,1H),8.34(d,J＝4.0Hz,1H),7.78-7.24(m,5H),5.11(s,1H),5.01(s,1H),4.06(m,2H),2.83-2.52(m,4H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝550.3。

By following the same procedure as above, compound 2 substituted with different R can be obtained by compound 2b with different R, keeping other conditions unchanged. R is-OC₁₈H₃₃,-OC₁₉H₃₉,-OC₂₇H₄₅,-OC₃₇H₆₇,-OC₅₉H₁₁₉,-OC₆₁H₁₁₅,-OC₆₁H₁₂₁。

Example 3 Synthesis of Compound 3

Step 1: synthesis of Compound 3b

Dissolve 3a (5.0g,27.3mmol) in N, N-dimethylformamide (100.0mL) at room temperature, add potassium carbonate (7.5g,54.6mmol), 1-bromooctadecane (13.6g,40.95mmol), stir the reaction mixture at 50 ℃ for 8 hours, add water to the reaction, extract with dichloromethane, dry concentrate to give crude product, add methanol to the crude product and filter to give 3b (5.9g, 13.6mmol) as a white solid in 50% yield.¹H-NMR(400MHz,CDCl₃)δ＝10.01(s,1H),8.44(d,J＝4.0Hz,1H),8.34(d,J＝4.0Hz,1H),7.78-7.24(m,5H),4.06(m,2H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝436.3。

Step 2: synthesis of Compound 3c

Dissolving 3b (5.9g, 13.6mmol) in N, N-dimethylformamide (120.0mL) at room temperature, adding potassium hydroxide (2.1g,38.2mmol) and bromoethanol (4.7g,38.2mmol), and stirring the reaction mixture at 50 ℃ for 6 hours; water was added to the reaction solution, extracted with dichloromethane, dried and concentrated to give the crude product, which was filtered by addition of methanol to give 3c (4.5g, 9.5mmol) as a white solid in 70% yield.¹H-NMR(400MHz,CDCl₃)δ＝10.01(s,1H),8.44(d,J＝4.0Hz,1H),8.34(d,J＝4.0Hz,1H),7.78-7.24(m,5H),4.47(m,2H),4.06(m,2H),3.63(m,2H),3.62(s,1H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝480.4。

And step 3: synthesis of Compound 3

3c (4.5g, 9.5mmol) was dissolved in anhydrous dichloromethane (90.0mL) at room temperature, triethylamine (1.9g,19.0mmol), 4-dimethylaminopyridine (2.3g,19.0mmol), succinic anhydride (1.4g,14.2mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, dried and concentrated to give a crude product, which was filtered by adding methanol to give 3(4.4g, 7.6mmol) as a white solid in 80% yield.¹H-NMR(400MHz,CDCl₃)δ＝10.01(s,1H),8.44(d,J＝4.0Hz,1H),8.34(d,J＝4.0Hz,1H),7.78-7.24(m,5H),4.47(m,2H),4.06(m,2H),3.63(m,2H),3.62(s,1H),2.83-2.52(m,4H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝580.4。

By following the same procedure as described above, with other conditions being maintained, different alkyl bromides are added in step 1 to give compounds 3 with different R substitutions. R is-OC₃₇H₆₇。

Example 4 Synthesis of Compound 4

Step 1: synthesis of Compound 4b

4a (5.0g,23.5mmol) was dissolved in N, N-dimethylformamide at room temperature(150.0mL), potassium carbonate (16.5g,117.5mmol), 1-bromooctadecane (11.7g,35.2mmol) were added, the reaction mixture was stirred at 80 ℃ for 15 hours, water was added to the reaction mixture, dichloromethane was used for extraction, drying and concentration were carried out to obtain a crude product, and methanol was added to the crude product to obtain 4b (10.1g, 14.1mmol) as a white solid in 60% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.76(d,J＝4.0Hz,2H),7.06(d,J＝4.0Hz,2H),6.89(d,J＝4.0Hz,2H),4.06(m,4H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝717.6。

Step 2: synthesis of Compound 4c

Dissolve 4b (10.1g, 14.1mmol) in methanol (200.0mL) at room temperature, add sodium borohydride (803mg,21.1mmol) under ice bath, stir the reaction mixture at 0 ℃ for 0.5 h, add saturated aqueous ammonium chloride to the reaction, extract with dichloromethane, dry concentrate to give crude product, add methanol to the crude product and filter to give 4c (9.1g, 12.6mmol) as a white solid in 90% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.76(d,J＝4.0Hz,2H),7.06(d,J＝4.0Hz,2H),6.89(d,J＝4.0Hz,2H),6.43(s,1H),5.60(s,1H),4.06(m,4H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝719.6。

And step 3: synthesis of Compound 4

4c (9.1g, 12.6mmol) was dissolved in anhydrous dichloromethane (180.0mL) at room temperature, triethylamine (2.5g,25.2mmol), 4-dimethylaminopyridine (3.1g,25.2mmol), succinic anhydride (1.9g,18.9mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, dried and concentrated to give a crude product, which was filtered by adding methanol to give 4(8.7g, 10.7mmol) as a white solid in 85% yield.¹H-NMR(400MHz,CDCl₃)δ＝11.12(s,1H),7.76(d,J＝4.0Hz,2H),7.06(d,J＝4.0Hz,2H),6.89(d,J＝4.0Hz,2H),6.43(s,1H),5.60(s,1H),4.06(m,4H),2.83-2.52(m,4H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝819.6。

By following the same procedure as described above, with other conditions being maintained, different alkyl bromides are added in step 1 to give compounds 4 substituted with different R. R is-OC₂₇H₄₅，-OC₃₇H₆₇，-OC₆₁H₁₁₅。

Example 5 Synthesis of Compound 5

Step 1: synthesis of Compound 5c

4b1(10.5g,11.1mmol) was dissolved in methanol (200.0mL) at room temperature, ammonium formate (7.0g,111mmol), 10% palladium on carbon (1.05g) were added, the reaction mixture was stirred at room temperature under nitrogen for 15 hours, the reaction was filtered and washed with dichloromethane 3 times, the mother liquor was concentrated to give crude product, which was filtered to give 5c (6.3g, 6.66mmol) as a white solid in 60% yield by adding methanol.¹H-NMR(400MHz,CDCl₃)δ＝7.76(d,J＝4.0Hz,2H),7.06(d,J＝4.0Hz,2H),6.89(d,J＝4.0Hz,2H),5.11(s,1H),5.01(s,1H),4.06(m,4H),1.76(m,4H),1.43-1.31(m,100H),0.88(m,6H)；MS(ESI)m/z＝950.7。

Step 2: synthesis of Compound 5

5c (6.3g, 6.66mmol) was dissolved in anhydrous dichloromethane (120.0mL) at room temperature, triethylamine (1.3g,13.3mmol), 4-dimethylaminopyridine (1.6g,13.3mmol), succinic anhydride (9.9g,9.99mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, dried and concentrated to give a crude product, which was filtered by adding methanol to give 5(5.5g, 5.3mmol) as a white solid in 80% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.76(d,J＝4.0Hz,2H),7.06(d,J＝4.0Hz,2H),6.89(d,J＝4.0Hz,2H),5.11(s,1H),5.01(s,1H),4.06(m,4H),2.83-2.52(m,4H),1.76(m,4H),1.43-1.31(m,100H),0.88(m,6H)；MS(ESI)m/z＝1050.7。

Example 6 Synthesis of Compound 6

Step 1: synthesis of Compound 6b

6a (2.2g,11.1mmol) was dissolved in tetrahydrofuran (50.0mL) at room temperature and triphenylphosphine (5.8g, 22.1 mmol) was added2mmol), cholesterol (4.2g,11.1mmol), diisopropyl azodicarboxylate (4.5g,22.2mmol) was added under ice bath, the reaction mixture was stirred at room temperature for 15 hours under nitrogen protection, water was added to the reaction solution, dichloromethane was extracted and concentrated to give crude product, which was filtered by adding methanol to give 6b (6.2g, 6.66mmol) as a white solid in 60% yield.¹H-NMR(400MHz,CDCl₃)δ＝10.01(s,1H),7.76(d,J＝4.0Hz,2H),7.06(d,J＝4.0Hz,2H),6.89(d,J＝4.0Hz,2H),4.06(m,4H),1.76(m,4H),1.43-1.31(m,100H),0.88(m,6H)；MS(ESI)m/z＝936.7。

Step 2: synthesis of Compound 6

6b (6.2g, 6.66mmol) was dissolved in anhydrous dichloromethane (120.0mL) at room temperature, triethylamine (1.3g,13.3mmol), 4-dimethylaminopyridine (1.6g,13.3mmol), succinic anhydride (9.9g,9.99mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, drying was concentrated to give a crude product, and the crude product was filtered by adding methanol to give 6(5.5g, 5.3mmol) as a white solid in 80% yield.¹H-NMR(400MHz,CDCl₃)δ＝10.01(s,1H),7.76(d,J＝4.0Hz,2H),7.06(d,J＝4.0Hz,2H),6.89(d,J＝4.0Hz,2H),4.06(m,4H),2.83-2.52(m,4H),1.76(m,4H),1.43-1.31(m,100H),0.88(m,6H)；MS(ESI)m/z＝1036.7。

Example 7 Synthesis of Compound 7

Step 1: synthesis of Compound 7c

Dissolve 6b (17.8g, 19.11mmol) in N, N-dimethylformamide (356.0mL) at room temperature, add potassium hydroxide (2.1g,38.2mmol), bromoethanol (4.7g,38.2mmol), stir the reaction mixture at 50 ℃ for 6 hours, add water to the reaction mixture, extract with dichloromethane, dry concentrate to give crude product, add methanol to the crude product and filter to give 7c (13.1g, 13.3mmol) as a white solid in 70% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.76(d,J＝4.0Hz,2H),7.06(d,J＝4.0Hz,2H),6.89(d,J＝4.0Hz,2H),4.47(m,2H),4.06(m,2H),3.63(m,2H),3.62(s,1H),1.76(m,4H),1.43-1.31(m,100H),0.88(m,6H)；MS(ESI)m/z＝980.7。

Step 2: synthesis of Compound 7

7c (13.1g, 13.3mmol) was dissolved in anhydrous dichloromethane (260.0mL) at room temperature, triethylamine (2.6g,26.6mmol), 4-dimethylaminopyridine (3.2g,26.6mmol), succinic anhydride (1.9g,19.9mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, dried and concentrated to give a crude product, which was filtered by adding methanol to give 7(11.4g, 10.6mmol) as a white solid in 80% yield.¹H-NMR(400MHz,CDCl₃)δ＝11.12(s,1H),7.76(d,J＝4.0Hz,2H),7.06(d,J＝4.0Hz,2H),6.89(d,J＝4.0Hz,2H),4.47(m,2H),4.06(m,2H),3.63(m,2H),3.62(s,1H),2.83-2.52(m,4H),1.76(m,4H),1.43-1.31(m,100H),0.88(m,6H)；MS(ESI)m/z＝1080.7。

Example 8 Synthesis of Compound 8

Step 1: synthesis of Compound 8b

Dissolve 8a (5.2g,35.7mmol) in N, N-dimethylformamide (50.0mL) at room temperature, add potassium carbonate (24.6g,178.5mmol), 1-bromooctadecane (17.8g,53.5mmol), stir the reaction mixture at 50 ℃ for 15 hours, add water to the reaction, extract with dichloromethane, dry concentrate to give crude product, add methanol to the crude product and filter to give 8b (9.8g, 24.5mmol) as a white solid in 70% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.09(d,J＝4.0Hz,1H),6.71(m,2H),2.86(m,2H),2.31(m,2H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝401.3。

Step 2: synthesis of Compound 8c

Dissolve 8b (9.8g, 24.5mmol) in methanol (200.0mL) at room temperature, add sodium borohydride (1.4g,36.7mmol) under ice bath, stir the reaction mixture at 0 deg.C for 0.5 h, add saturated aqueous ammonium chloride to the reaction, extract with dichloromethane, dry concentrate to give crude, add methanol to the crude and filter to give 8c (8.9g, 22.05mmol) as a white solid in 90% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.09(d,J＝4.0Hz,1H),6.71(m,2H),4.64(m,1H),4.06(m,2H),2.86(m,2H),2.31(m,2H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝403.3。

And step 3: synthesis of Compound 8

8c (8.9g, 22.05mmol) was dissolved in anhydrous dichloromethane (180.0mL) at room temperature, triethylamine (5.5g,55.1mmol), 4-dimethylaminopyridine (6.7g,55.1mmol), succinic anhydride (3.3g,33.1mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, dried and concentrated to give a crude product, which was filtered by adding methanol to give 8(9.4g, 18.7mmol) as a white solid in 85% yield.¹H-NMR(400MHz,CDCl₃)δ＝11.12(s,1H),7.09(d,J＝4.0Hz,1H),6.71(m,2H),4.64(m,1H),4.06(m,2H),3.65(m,1H),2.86(m,2H),2.83-2.52(m,4H),2.31(m,2H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝503.3。

By following the same procedure as described above, with other conditions being maintained, different alkyl bromides are added in step 1 to give compounds 8 substituted with different R. R is-OC₂₇H₄₅，-OC₃₇H₆₇，-OC₆₁H₁₁₅。

Example 9 Synthesis of Compound 9

Step 1: synthesis of Compound 9b

9a (1.8g,11.1mmol) was dissolved in tetrahydrofuran (40.0mL) at room temperature, triphenylphosphine (5.8g,22.2mmol), (6Z,9Z,28Z,31Z) -heptatriaconta-6, 9,28, 31-tetraene-19-methanol (6.3g,11.1mmol) was added, diisopropyl azodicarboxylate (4.5g,22.2mmol) was added under ice bath, the reaction mixture was stirred at room temperature for 15 hours under nitrogen protection, water was added to the reaction solution, dichloromethane was extracted and concentrated to give crude product, and methanol was added to the crude product to give 9b (4.5g, 6.66mmol) as a white solid in 60% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.09(d,J＝4.0Hz,1H),6.71(m,2H),5.43(m,8H),2.86(m,2H),2.83-2.52(m,4H),2.50(m,2H),2.31(m,2H),1.76-1.31(m,54H),0.88(m,6H)；MS(ESI)m/z＝673.5。

Step 2: synthesis of Compound 9c

Dissolve 9b (4.5g, 6.66mmol) in methanol (90.0mL) at room temperature, add sodium borohydride (303mg,7.9mmol) under ice bath, stir the reaction mixture at 0 ℃ for 0.5 h, add saturated aqueous ammonium chloride to the reaction, extract with dichloromethane, dry concentrate to give crude product, add methanol to the crude product and filter to give 9c (4.0g, 6.0mmol) as a white solid in 90% yield. Ms (esi) m/z 675.6.

And step 3: synthesis of Compound 9

9c (4.0g, 6.0mmol) was dissolved in anhydrous dichloromethane (80.0mL) at room temperature, triethylamine (1.2g,12.0mmol), 4-dimethylaminopyridine (1.5g,12.0mmol), succinic anhydride (900mg,9.0mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, dried and concentrated to give a crude product, which was filtered by adding methanol to give 9(3.9g, 5.1mmol) as a white solid in 85% yield.¹H-NMR(400MHz,CDCl₃)δ＝11.12(s,1H),7.09(d,J＝4.0Hz,1H),6.71(m,2H),5.43(m,8H),3.65(m,1H),2.86(m,2H),2.83-2.52(m,4H),2.50(m,2H),2.31(m,2H),1.76-1.31(m,54H),0.88(m,6H)；MS(ESI)m/z＝775.6。

Example 10 Synthesis of Compound 10

Step 1: synthesis of Compound 10b

10a (5.8g,35.7mmol) was dissolved in N, N-dimethylformamide (110.0mL) at room temperature, potassium carbonate (24.6g,178.5mmol), 1-bromooctadecane (23.7g,71.4mmol) were added, the reaction mixture was stirred at 50 ℃ for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, drying and concentration were carried out to obtain a crude product, and the crude product was filtered by adding methanol to obtain 10b (14.3g, 21.4mmol) as a white solid in 60% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.09(d,J＝4.0Hz,2H),2.86(m,2H),2.31(m,2H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝669.6。

Step 2: synthesis of Compound 10c

Dissolve 10b (14.3g, 21.4mmol) in methanol (280.0mL) at room temperature, add sodium borohydride (1.2g,32.1mmol) under ice bath, stir the reaction mixture at 0 ℃ for 0.5 h, add saturated aqueous ammonium chloride to the reaction, extract with dichloromethane, dry concentrate to give crude product, add methanol to the crude product and filter to give 10c (12.9g, 19.2mmol) as a white solid in 90% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.09(d,J＝4.0Hz,2H),4.64(m,1H),4.04(m,1H),2.86(m,2H),2.31(m,2H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝671.6。

And step 3: synthesis of Compound 10

10c (12.9g, 19.2mmol) was dissolved in anhydrous dichloromethane (200.0mL) at room temperature, triethylamine (3.8g,38.4mmol), 4-dimethylaminopyridine (4.6g,38.4mmol), succinic anhydride (2.8g,28.8mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, the reaction mixture was added with water, dichloromethane was extracted, dried and concentrated to give a crude product, which was filtered with methanol to give 10(14.8g, 16.3mmol) as a white solid in 85% yield.¹H-NMR(400MHz,CDCl₃)δ＝11.12(s,1H),7.09(d,J＝4.0Hz,2H),4.64(m,1H),2.86(m,2H),2.83-2.52(m,4H),2.31(m,2H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝771.6。

Example 11 Synthesis of Compound 11

Step 1: synthesis of Compound 11b

11a (6.3g,35.7mmol) was dissolved in N, N-dimethylformamide (120.0mL) at room temperature, potassium carbonate (24.6g,178.5mmol), 1-bromooctadecane (23.7g,71.4mmol) were added, the reaction mixture was stirred at 50 ℃ for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, drying and concentration were carried out to obtain a crude product, and the crude product was filtered by adding methanol to obtain 11b (16.5g, 24.5mmol) as a white solid in 70% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.09(d,J＝4.0Hz,2H),2.86(m,2H),2.83-2.52(m,4H),2.50(m,2H),2.31(m,2H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝683.6。

Step 2: synthesis of Compound 11c

11b (16.5g, 24.5mmol) was dissolved in methanol (240.0mL) at room temperature, sodium borohydride (1.4g,36.7mmol) was added under ice-bath, and the reaction mixture was stirred at 0 ℃ for 0.5 h. Saturated aqueous ammonium chloride was added to the reaction solution, extracted with dichloromethane, dried and concentrated to give the crude product, which was filtered with methanol to give 11c (15.1g, 22.05mmol) as a white solid in 90% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.09(d,J＝4.0Hz,2H),4.64(m,1H),4.01(m,1H),2.86(m,2H),2.83-2.52(m,4H),2.50(m,2H),2.31(m,2H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝685.6。

And step 3: synthesis of Compound 11

11c (15.1g, 22.05mmol) was dissolved in dry dichloromethane (300.0mL) at room temperature, triethylamine (5.5g,55.1mmol), 4-dimethylaminopyridine (6.7g,55.1mmol), succinic anhydride (3.3g,33.1mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, dried and concentrated to give a crude product, which was filtered by adding methanol to give 11(14.6g, 18.7mmol) as a white solid in 85% yield.¹H-NMR(400MHz,CDCl₃)δ＝11.12(s,1H),7.09(d,J＝4.0Hz,2H),4.64(m,1H),2.86(m,2H),2.83-2.52(m,4H),2.50(m,2H),2.31(m,2H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝785.6。

Example 12 Synthesis of Compound 12

Step 1: synthesis of Compound 12b

Dissolving 12a (5.0g,22.5mmol) in anhydrous dichloromethane (50.0mL) at room temperature, slowly adding boron tribromide in dichloromethane (78.8mL,78.8mmol,1M/DCM) at-30 deg.C, stirring the reaction mixture at-30 deg.C for 2 hr, slowly adding saturated aqueous sodium bicarbonate solution to neutralize to neutrality, extracting with dichloromethane, drying and concentrating to obtain crude product 12b (2.8g )15.7mmol) of white solid, 70% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.09(s,1H),5.35(m,3H),2.86(m,2H),2.31(m,2H)；MS(ESI)m/z＝181.0。

Step 2: synthesis of Compound 12c

12b (2.8g, 15.7mmol) was dissolved in N, N-dimethylformamide (100.0mL) at room temperature, potassium carbonate (31g,225.0mmol), 1-bromooctadecane (18.2g,54.9mmol) were added, the reaction mixture was stirred at 50 ℃ for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, drying was concentrated to give crude product, which was filtered by adding methanol to give 12c (8.8g,9.4mmol) as a white solid in 60% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.09(s,1H),2.86(m,2H),2.31(m,2H),1.76(m,6H),1.43-1.31(m,90H),0.88(m,9H)；MS(ESI)m/z＝937.8。

And step 3: synthesis of Compound 12d

12c (8.8g,9.4mmol) was dissolved in methanol (163.0mL) at room temperature, sodium borohydride (535mg,14.1mmol) was added under ice-bath, the reaction mixture was stirred at 0 ℃ for 0.5 h, saturated aqueous ammonium chloride was added, dichloromethane was extracted, dried and concentrated to give the crude product, which was filtered by adding methanol to give 12d (7.9g, 8.46mmol) as a white solid in 90% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.09(s,1H),4.64(m,1H),4.01(m,1H),2.86(m,2H),2.31(m,2H),1.76(m,6H),1.43-1.31(m,90H),0.88(m,9H)；MS(ESI)m/z＝939.9。

And 4, step 4: synthesis of Compound 12

12d (7.9g, 8.46mmol) was dissolved in anhydrous dichloromethane (160.0mL) at room temperature, triethylamine (1.7g,16.9mmol), 4-dimethylaminopyridine (2.1g,55.1mmol), succinic anhydride (1.2g,12.6mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, drying was concentrated to give a crude product, the crude product was filtered by adding methanol to give 12(7.1g, 6.7mmol) as a white solid in 80% yield.¹H-NMR(400MHz,CDCl₃)δ＝11.12(s,1H),7.09(s,1H),4.64(m,1H),2.86(m,2H),2.83-2.52(m,4H),2.31(m,2H),1.76(m,6H),1.43-1.31(m,90H),0.88(m,9H)；MS(ESI)m/z＝1039.9。

Example 13 Synthesis of Compound 13

Step 1: synthesis of Compound 13b

Dissolving 13a (2.0g,15.0mmol) in N, N-dimethylformamide (50.0mL) at room temperature, adding potassium carbonate (10.3g,75.1mmol) and 1-bromooctadecane (7.5g,22.5mmol), stirring the reaction mixture at 50 ℃ for 15 hours, adding water to the reaction solution, extracting with dichloromethane, drying and concentrating to obtain a crude product, adding methanol to the crude product, and filtering to obtain 13b (2.9g, 7.5mmol) as a white solid with a yield of 50%.¹H-NMR(400MHz,CDCl₃)δ＝10.1(m,1H),7.78-7.24(m,5H),4.06(m,2H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝386.3。

Step 2: synthesis of Compound 13c

Dissolving 13b (2.9g, 7.5mmol) in N, N-dimethylformamide (50.0mL) at room temperature, adding potassium hydroxide (3.1g,22.5mmol) and bromoethanol (2.8g,22.5mmol), stirring the reaction mixture at 50 ℃ for 6 hours, adding water to the reaction mixture, extracting with dichloromethane, drying and concentrating to obtain a crude product, adding methanol to the crude product, and filtering to obtain 13c (2.2g, 5.2mmol) as a white solid with a yield of 70%.¹H-NMR(400MHz,CDCl₃)δ＝7.78-7.24(m,5H),4.47(m,2H),4.06(m,2H),3.63(m,2H),3.61(m,1H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝430.3。

And step 3: synthesis of Compound 13

13c (2.2g, 5.2mmol) was dissolved in anhydrous dichloromethane (50.0mL) at room temperature, triethylamine (1.0g,10.4mmol), 4-dimethylaminopyridine (1.2g,10.4mmol), succinic anhydride (0.78g,7.8mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, dried and concentrated to give a crude product, which was filtered by adding methanol to give 13(2.2g, 4.16mmol) as a white solid in 80% yield.¹H-NMR(400MHz,CDCl₃)δ＝11.01(s,1H),7.78-7.24(m,5H),4.47(m,2H),4.06(m,2H),3.63(m,2H),2.83-2.53(m,4H),1.76(m,2H),1.43-1.31(m,30H),0.88(m,3H)；MS(ESI)m/z＝530.3。

Example 14 Synthesis of Compound 14

Step 1: synthesis of Compound 14b

Dissolve 14a (3.0g,20.1mmol) in N, N-dimethylformamide (60.0mL) at room temperature, add potassium carbonate (13.9g,100.5mmol), 1-bromooctadecane (13.6g,40.95mmol), stir the reaction mixture at 50 ℃ for 15 hours, add water to the reaction, extract with dichloromethane, dry concentrate to give crude product, add methanol to the crude product and filter to give 14b (6.5g, 10.0mmol) as a white solid in 50% yield.¹H-NMR(400MHz,CDCl₃)δ＝10.1(m,1H),7.78-7.24(m,4H),4.06(m,4H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝654.6。

Step 2: synthesis of Compound 14c

14b (6.5g, 10.0mmol) was dissolved in N, N-dimethylformamide (130.0mL) at room temperature, potassium hydroxide (2.1g,38.2mmol) and bromoethanol (4.7g,38.2mmol) were added, and the reaction mixture was stirred at 50 ℃ for 6 hours. Water was added to the reaction mixture, extracted with dichloromethane, dried and concentrated to give crude which was filtered with methanol to give 14c (4.9g, 7.0mmol) as a white solid in 70% yield.¹H-NMR(400MHz,CDCl₃)δ＝7.78-7.24(m,4H),4.06(m,4H),4.06(m,2H),3.63(m,2H),3.61(m,1H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝698.6。

And step 3: synthesis of Compound 14

14c (4.9g, 7.0mmol) was dissolved in dry dichloromethane (100.0mL) at room temperature, triethylamine (1.4g,14.0mmol), 4-dimethylaminopyridine (1.7g,14.0mmol), succinic anhydride (1.1g,10.5mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, water was added to the reaction mixture, dichloromethane was extracted, dried and concentrated to give a crude product, which was filtered by adding methanol to give 14(8.5g, 10.6mmol) as a white solid in 80% yield.¹H-NMR(400MHz,CDCl₃)δ＝11.01(s,1H),7.78-7.24(m,4H),4.06(m,4H),4.06(m,2H),3.63(m,2H),3.61(m,1H),2.83-2.53(m,4H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝798.6。

Example 15 Synthesis of Compound 15

Compound 4(5.0g,6.1mmol) was dissolved in dichloromethane (50.0mL) at room temperature, 4-dimethylaminopyridine (1.1g,9.1mmol), diisopropylethylamine (1.1g,9.1mmol), benzotriazole-N, N, N ', N ' -tetramethylurea hexafluorophosphate (3.4g,9.1mmol), N-acetyl-5 ' -O- (4,4' -dimethoxytrityl) -2' -deoxycytidine (4.2g,7.3mmol) were added, the reaction mixture was stirred at room temperature for 15 hours, 200.0mL of methanol was added to the reaction mixture to precipitate a solid, and the mixture was filtered to obtain compound 15(7.5g, 5.5mmol) as a white solid in 90% yield.¹H-NMR(400MHz,CDCl₃)δ＝10.12(s,1H),7.76(d,J＝4.0Hz,2H),7.80-7.12(m,15H),7.06(d,J＝4.0Hz,2H),6.89(d,J＝4.0Hz,2H),6.52(m,1H),6.43(s,1H),5.60(s,1H),4.56(m,1H),4.06(m,4H),3.89(m,1H),3.63(s,6H),3.01-2.89(m,2H),2.45-2.41(m,2H),2.83-2.52(m,4H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝1372.8。

Example 16 Synthesis of Compound 16

Compound 15(7.5g, 5.5mmol) was dissolved in 3% (v/v) dichloroacetic acid/dichloromethane solution (75.0mL) at room temperature, pyrrole (3.6g,55.0mmol) was added, the reaction mixture was stirred at room temperature for 30 minutes, pyridine (2.1mL,27.0mmol) was added to the reaction mixture to neutralize, and then 7.5g molecular sieve, N-acetyl-5 '-O- (4,4' -dimethoxytrityl) -2 '-deoxycytidine-3' - [ O- (2-cyanoethyl) - (N, N-diisopropyl) was added]Adding methylene chloride solution of phosphoramidite (8.5g,11.0mmol) into the reaction solution, stirring the reaction mixture at room temperature for 15 min, adding 0.25M 5-ethylthio tetrazole/acetonitrile (88.0mL,22.0mmol), stirring the reaction mixture at room temperature for 1 h, adding 0.2M iodine/pyridine (50.0 mL)/water (5.0mL) solution, stirring at room temperature for 10 min, and adding saturated sulfurous acidStirring the sodium methanol solution for 10 minutes at room temperature; 500.0mL of dichloromethane was added to the reaction mixture, the mixture was filtered to obtain a filtrate, the filtrate was concentrated and methanol was added to precipitate a solid, which was filtered to obtain 16(8.7g, 4.95mmol) as a white solid in 90% yield.¹H-NMR(400MHz,CDCl₃)δ＝10.12(s,2H),7.76(d,J＝4.0Hz,2H),7.80-7.12(m,17H),7.06(d,J＝4.0Hz,2H),6.89(d,J＝4.0Hz,2H),6.52(m,2H),6.43(s,1H),5.60(s,1H),4.56(m,2H),4.06(m,4H),3.89(m,2H),3.63(s,12H),3.01-2.89(m,4H),2.45-2.41(m,8H),2.83-2.52(m,4H),1.76(m,4H),1.43-1.31(m,60H),0.88(m,6H)；MS(ESI)m/z＝1756.9。

Example 17 Synthesis of Compound 17

The procedure of example 16 was repeated 15 times by replacing N-acetyl-5 '-O- (4,4' -dimethoxytrityl) -2 '-deoxycytidine-3' - [ O- (2-cyanoethyl) - (N, N-diisopropyl) ] -phosphoramidite with the corresponding different starting material to give compound 17(7.4 g).

Example 18 Synthesis of Compound 18

Adding compound 17(7.4g) into 50.0mL ethanol at room temperature, adding 100.0mL 37% (wt%) methylamine aqueous solution, reacting the reaction mixture at 45 ℃ for 1 hour, adding dichloromethane for extraction for 3 times, freeze-drying the aqueous phase to obtain compound 18, HPLC refining the compound 18 crude raw material by GE Source 15Q ion exchange column chromatography (1.5M NaBr/40mM Tris-HBr buffer pH 8.0), mixing qualified product segments after refining, desalting, and freeze-drying to obtain the target compound. Ms (esi) m/z 4841.9.

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