阅读说明：本技术 一种间充质干细胞储藏盒 (Mesenchymal stem cell storage box ) 是由 刘荣怀 杨西岐 吕雪雅 于 2021-09-06 设计创作，主要内容包括：本发明涉及间充质干细胞储藏技术领域,具体的说是一种间充质干细胞储藏盒,包括盒体、控温单元,还包括驱动单元、螺纹杆、储藏盒和控制器；所述盒体为圆柱形,盒体内设置有圆柱空腔；所述盒体的上表面设置有环形槽；两根所述螺纹杆的另一端穿过盒体延伸至盒体外部固连着一号齿轮,两根螺纹杆与盒体之间转动密封连接,两根螺纹杆之间设有驱动单元；所述盒体下方固连有保护壳；本发明通过控温单元控制间充质干细胞的储藏温度和驱动单元控制螺纹杆从而控制储藏盒的升降之间相配合,使得工作人员对间充质干细胞存储袋的取用更为方便,同时,控温单元设置在储藏盒的内部使得冷量不易流失,提高了冷量利用率,从而提高了本发明的实用性。(The invention relates to the technical field of mesenchymal stem cell storage, in particular to a mesenchymal stem cell storage box, which comprises a box body, a temperature control unit, a driving unit, a threaded rod, a storage box and a controller, wherein the box body is provided with a box body; the box body is cylindrical, and a cylindrical cavity is arranged in the box body; the upper surface of the box body is provided with an annular groove; the other ends of the two threaded rods penetrate through the box body and extend to the outside of the box body, a first gear is fixedly connected with the outside of the box body, the two threaded rods are in rotary sealing connection with the box body, and a driving unit is arranged between the two threaded rods; a protective shell is fixedly connected below the box body; according to the invention, the storage temperature of the mesenchymal stem cells is controlled by the temperature control unit and the lifting of the storage box is controlled by controlling the threaded rod by the driving unit, so that the mesenchymal stem cell storage bag can be taken by a worker more conveniently, meanwhile, the temperature control unit is arranged in the storage box, so that the cold energy is not easy to lose, the utilization rate of the cold energy is improved, and the practicability of the invention is improved.)

1. The utility model provides a mesenchymal stem cell storage box, includes box body (1), accuse temperature unit, its characterized in that: the device also comprises a driving unit, a threaded rod (12), a storage box (11) and a controller; the box body (1) is cylindrical, and a cylindrical cavity (18) is arranged in the box body (1); a temperature control unit is arranged in the cylindrical cavity (18); an annular groove (112) is formed in the upper surface of the box body (1); the annular groove (112) surrounds the cylindrical cavity (18), and the storage box (11) is connected in the annular groove (112) in a sliding manner; a sealing cover (7) is arranged above the storage box (11), and two threaded rods (12) are connected with the storage box (11) through internal threads; the other ends of the two threaded rods (12) penetrate through the box body (1) and extend to the outside of the box body (1) to be fixedly connected with a first gear (27), the two threaded rods (12) are connected with the box body (1) in a rotating and sealing mode, and a driving unit is arranged between the two threaded rods (12); the drive unit can control the rotation of the two threaded rods (12); a protective shell (2) is fixedly connected below the box body (1); the protective shell (2) wraps the other end of the threaded rod (12) and the driving unit.

2. A mesenchymal stem cell storage box according to claim 1, wherein: the temperature control unit comprises an annular pipeline (17), a first air inlet pipe (13), a second air inlet pipe (14), a first air outlet pipe (15) and a second air outlet pipe (16); the upper surface of the box body (1) is fixedly connected with a first air inlet pipe (13), a second air inlet pipe (14), a second air outlet pipe (16) and a first air outlet pipe (15); the first air inlet pipe (13), the second air inlet pipe (14), the second air outlet pipe (16) and the first air outlet pipe (15) penetrate through the box body (1) and are communicated with the cylindrical cavity (18); an annular pipeline (17) is fixedly connected between the first air inlet pipe (13) and the first air outlet pipe (15); the annular duct (17) is located inside a cylindrical cavity (18).

3. A mesenchymal stem cell storage box according to claim 1, wherein: the driving unit comprises a motor (21), a first gear (27) and a second gear (22); the motor (21) is fixedly connected with the lower surface of the box body (1), and the output end of the motor (21) is fixedly connected with a first gear (27); the first gear (27) is meshed with two second gears (22); the two second gears (22) are fixedly connected with one ends, far away from the box body (1), of the two threaded rods (12) respectively.

4. A mesenchymal stem cell storage box according to claim 1, wherein: a vacuum layer (5) is arranged in the box body (1); the vacuum layer (5) encloses the annular groove (112) and the cylindrical cavity (18).

5. A mesenchymal stem cell storage box according to claim 1, wherein: the storage box (11) is in a ring barrel shape; the outer wall of the storage box (11) is uniformly provided with storage grooves (111), and the part of the storage box (11) connected with the threaded rod (12) is solid.

6. A mesenchymal stem cell storage box according to claim 5, wherein: the upper surface of the storage box (11) is fixedly connected with the sealing cover (7).

7. A mesenchymal stem cell storage box according to claim 6, wherein: the storage groove (111) is hinged to a turnover cover (8) through a torsion spring (82) and close to the outer edge of the protective shell (2); one surface of the turnover cover (8) close to the storage groove (111) is fixedly connected with a pull rope (81); the other end of the pull rope (81) is fixedly connected with one surface, far away from the protective shell (2), of the storage groove (111).

8. A mesenchymal stem cell storage box according to claim 7, wherein: an arc-shaped slope (4) is arranged at the corner of the notch of the annular groove, which is close to the box body (1); an annular gasket (3) is arranged at the joint of the sealing cover (7) and the box body (1); the annular gasket (3) is fixedly connected with the box body (1).

9. A mesenchymal stem cell storage box according to claim 8, wherein: a through hole (6) is formed in the groove bottom of the annular groove (112) close to the cylindrical cavity (18); the through hole (6) communicates the annular groove (112) with the cylindrical cavity (18).

10. A mesenchymal stem cell storage box according to claim 1, wherein: a first bevel gear (23) is fixedly connected to the center of the first gear (27); the protective shell (2) is rotatably connected with a transmission rod (25); the transmission rod (25) can slide on the inner wall of the protective shell (2), one end, close to the first bevel gear (23), of the transmission rod (25) extends to the inside of the protective shell (2) and is fixedly connected with the second bevel gear (24), and one end, far away from the second bevel gear (24), of the transmission rod (25) extends to the outside of the protective shell (2) and is fixedly connected with a handle (26); the sliding of the transmission rod (25) can enable the first bevel gear (23) and the second bevel gear (24) to be meshed.

Technical Field

The invention relates to the technical field of mesenchymal stem cell storage, in particular to a mesenchymal stem cell storage box.

Background

Mesenchymal stem cells are pluripotent stem cells that have all of the commonalities of stem cells, namely self-renewal and pluripotency; the application is most in clinical application, and the composition is combined with hematopoietic stem cells to improve the success rate of transplantation and accelerate hematopoietic reconstruction, and one multifunctional cell in tissues can be differentiated into a plurality of tissue cells; the mesenchymal stem cells can repair tissues and organs with living injury and pathological changes, have stronger immunoregulation effect, promote the recovery of hematopoietic function and store the mesenchymal stem cells; when the mesenchymal stem cells are frozen and preserved, the mesenchymal stem cell storage bag needs to be placed in a freezing tank for freezing and preservation, and a temperature control unit in the prior art is positioned outside a mesenchymal stem cell storage box, so that the cold source utilization rate of the preservation mode is low, and energy waste can be caused; meanwhile, the freezing tank needs to be opened to find the container when the container is taken, so that time and labor are wasted, and the time loss of workers is caused.

The Chinese patent with the application number of CN201820273449.9 discloses a dental pulp mesenchymal stem cell storage device, which comprises an outer tank body, an inner tank body and a nitrogen storage layer, wherein a heat insulation layer is arranged between the outer tank body and the nitrogen storage layer, the heat insulation layer is aluminum foil coated with paraffin, the nitrogen storage layer is arranged between the heat insulation layer and the inner tank body, the top parts of the outer tank body, the heat insulation layer, the inner tank body and the nitrogen storage layer are welded with a sealing ring body, the right side of the upper end of the sealing ring body is hinged with a circular sealing cover, the side end of the sealing cover is provided with a handle, an oxygen inlet pipe is arranged in a sedimentation hole arranged on the sealing cover in a threaded fit manner, the oxygen inlet pipe penetrates through the sedimentation hole to fix the sealing ring body, the lower end of the oxygen inlet pipe is connected with the sealing ring body in a threaded fit manner, sliding grooves are symmetrically arranged on the left side and the right side of the inner tank body, the technical scheme realizes the function of the block storage of the serial number of the dental pulp mesenchymal stem cells, and can realize the accurate serial number, has the advantages of simple structure, good freezing effect, etc.; but this technical scheme's temperature control unit is located mesenchymal stem cells's outside, and the cold source utilization ratio is low, and the process of taking mesenchymal stem cells is comparatively complicated to it is inhomogeneous to the cold-stored of mesenchymal stem cells, be unfavorable for mesenchymal stem cells's storage, thereby cause this technical scheme's limitation.

In view of the above, in order to overcome the above technical problems, the present invention provides a mesenchymal stem cell storage box, which solves the above technical problems.

Disclosure of Invention

In order to make up for the defects of the prior art, the invention provides the mesenchymal stem cell storage box, the mesenchymal stem cells are adjusted to be at proper temperature through the temperature control unit, and the storage box is controlled to be lifted and descended through the driving unit, so that the utilization rate of cooling capacity is improved, the energy waste is avoided, the mesenchymal stem cells are taken more conveniently, the working efficiency of workers is improved, and the invention has higher practicability.

The technical scheme adopted by the invention for solving the technical problems is as follows: a mesenchymal stem cell storage box comprises a box body, a temperature control unit, a driving unit, a threaded rod, a storage box and a controller; the box body is cylindrical, and a cylindrical cavity is arranged in the box body; a temperature control unit is arranged in the cylindrical cavity; the upper surface of the box body is provided with an annular groove; the annular groove surrounds the cylindrical cavity, and a storage box is connected in the annular groove in a sliding manner; a sealing cover is arranged above the storage box, and two threaded rods are connected with the inner thread of the storage box; the other ends of the two threaded rods penetrate through the box body and extend to the outside of the box body, a first gear is fixedly connected with the outside of the box body, the two threaded rods are in rotary sealing connection with the box body, and a driving unit is arranged between the two threaded rods; the driving unit can control the rotation of the two threaded rods; a protective shell is fixedly connected below the box body; the protective shell wraps the other end of the threaded rod and the driving unit; the controller is used for controlling the automatic operation of the mesenchymal stem cell storage box;

when the mesenchymal stem cell freezing storage method works, the mesenchymal stem cell storage bag needs to be placed in a freezing tank for freezing storage in the mesenchymal stem cell freezing storage process, and a temperature control unit in the prior art is positioned outside a mesenchymal stem cell storage box, so that the cold source utilization rate of the storage method is low, and energy waste can be caused; meanwhile, when the refrigerator is used, the refrigerator needs to be turned over, so that time and labor are wasted, and the time loss of workers is caused;

therefore, when the storage box is used by a worker, the worker controls the temperature control unit through the controller to enable the temperature in the storage chamber to meet the storage requirement of the mesenchymal stem cells, the controller controls the driving unit to rotate the threaded rod to lift the storage box, the worker puts the storage box into the mesenchymal stem cell storage bag, and at the moment, the controller controls the driving unit to reversely rotate the threaded rod to enable the storage box to descend and cover the sealing cover through manual operation to ensure sealing; when the staff need to take out the mesenchymal stem cell storage bag again, the staff controls the driving unit to drive the threaded rod to rotate by using the controller, so that the storage box is lifted; the temperature control unit is positioned in the storage box, and the cold energy is transmitted to the outside, so that the utilization rate of a cold source is higher and the energy is more fully utilized compared with the prior art in which the temperature control unit is arranged outside the storage box;

according to the invention, the storage temperature of the mesenchymal stem cells is controlled by the temperature control unit and the threaded rod is controlled by the driving unit so as to control the lifting of the storage box to be matched, so that the mesenchymal stem cell storage bag can be taken by a worker more conveniently, the time for taking the mesenchymal stem cells is saved, meanwhile, the temperature control unit is arranged in the storage box so that the cold energy is not easy to lose, the utilization rate of a cold source is improved, the use of the refrigerator is more economical, and the practicability of the refrigerator is further improved.

Preferably, the temperature control unit comprises an annular pipeline, a first air inlet pipe, a second air inlet pipe, a first air outlet pipe and a second air outlet pipe; the upper surface of the box body is fixedly connected with a first air inlet pipe, a second air outlet pipe and a first air outlet pipe; the first air inlet pipe, the second air outlet pipe and the first air outlet pipe penetrate through the box body and are communicated with the cylindrical cavity; an annular pipeline is fixedly connected between the first air inlet pipe and the first air outlet pipe; the annular pipeline is positioned inside the cylindrical cavity;

when the storage box works, the operator controls the second air outlet pipe to discharge air in the cylindrical cavity by using the controller, then the second air inlet pipe is slowly introduced with liquid nitrogen, meanwhile, the operator controls the first air inlet pipe to introduce oxygen into the annular pipeline to discharge air from the first air outlet pipe to form oxygen circulation, the liquid nitrogen generates physical liquefaction reaction in the cylindrical cavity to absorb heat and realize the cooling function, the arrangement of the oxygen annular pipeline can enable cold air to be in contact with the surface of the annular pipeline to realize temperature rise of the air in the cylindrical cavity, so that the temperature difference between the annular pipeline and the cylindrical cavity can be controlled by controlling the flow rate of the oxygen in the annular pipeline, the temperature in the cylindrical cavity is regulated, the application range of the storage box is enlarged, and meanwhile, the surface of the annular pipeline is uniformly in contact with the cold air in the cylindrical cavity when the temperature is regulated, therefore, the temperature regulation and control of the annular pipeline in each direction are synchronous, and the temperature transmitted to the storage box by the cylindrical cavity is uniform; according to the invention, liquid nitrogen enters the second air inlet pipe to cool the cylindrical cavity, and then the liquid nitrogen and oxygen entering the first air inlet pipe cooperate with regulation and control of the temperature in the cavity through the annular pipeline, so that the freezing of mesenchymal stem cells is realized, and the freezing temperature of the mesenchymal stem cells is ensured, thereby improving the practicability of the invention and facilitating the operation of workers.

Preferably, the driving unit comprises a motor, a first gear and a second gear; the motor is fixedly connected with the lower surface of the box body, and the output end of the motor is fixedly connected with a first gear; the first gear is meshed with two second gears; the two second gears are fixedly connected with one ends of the two threaded rods far away from the box body respectively;

when the device works, in the process of needing to take the mesenchymal stem cells, a worker controls the motor to rotate by using the controller, the motor rotates to drive the first gear to rotate, the first gear rotates to drive the second gear to rotate, so that the two threaded rods are driven to rotate, the threaded rods rotate to enable the storage box to ascend, when the storage box ascends to a position where the worker can take the mesenchymal stem cells out, the controller controls the motor to stop rotating, at the moment, the worker takes the mesenchymal stem cells needing to be used, the motor is controlled to reversely rotate by the controller to enable the storage box to move downwards until the storage box returns to the box body, the controller controls the motor to be closed, and the storage box stops moving; according to the invention, the first gear drives the second gear to rotate, so that the threaded rod is driven to rotate, the storage box can be automatically lifted by controlling the motor through the controller, convenience is provided for workers to take and place mesenchymal stem cells, and the invention is more reasonable.

Preferably, a vacuum layer is arranged in the box body; the vacuum layer wraps the annular groove and the cylindrical cavity; when the temperature control device works, the temperature in the storage box is transmitted by the temperature control unit in the cylindrical cavity, and the vacuum layer is arranged between the annular groove and the box body and between the cylindrical cavity and the box body, so that the temperature in the cylindrical cavity is reduced from being transmitted to the outside, the utilization rate of cold energy is increased, the energy waste is reduced, and the temperature control device has practical application effect.

Preferably, the storage box is shaped like a torus; the outer wall of the storage box is uniformly provided with storage grooves, and the part of the storage box connected with the threaded rod is solid; when the storage box works, the connecting part of the storage box and the threaded rod is solid, so that the storage of the mesenchymal stem cells is not influenced by the rotation of the threaded rod; meanwhile, the storage box stores the mesenchymal stem cells, and the mesenchymal stem cell storage bags with different numbers can be respectively placed in different storage tanks, so that the mesenchymal stem cell storage bags with specific numbers can be conveniently searched and taken out; according to the invention, the storage and the taking out of the mesenchymal stem cell storage bag are facilitated through the arrangement of the storage groove, so that the mesenchymal stem cell storage bag can be reasonably stored by workers, and the required mesenchymal stem cell storage bag can be quickly taken out, thereby improving the working efficiency of the workers and further bringing convenience to the workers.

Preferably, the upper surface of the storage box is fixedly connected with the sealing cover; when the storage box is in work, a worker controls the driving unit to rotate the threaded rod through the controller, so that the sealing cover is fixedly connected with the storage box in the downward moving process of the storage box, the storage box is sealed while the sealing cover moves, the step of manually closing the sealing cover by the worker is omitted, the operation process of the worker is omitted, the work efficiency of the worker is further improved, and the storage box has the characteristics of convenience and convenience in operation and simplicity in working process.

Preferably, the storage groove is hinged with a turnover cover through a torsion spring near the outer edge of the protective shell; one surface of the flip cover close to the storage groove is fixedly connected with a pull rope; the other end of the pull rope is fixedly connected with one surface of the storage groove, which is far away from the protective shell; when the storage box is in work, the flip cover on the storage groove is opened under the action of the torsion spring in the process that the storage box moves upwards, and the opening angle of the flip cover is limited by the arrangement of the pull rope; the mesenchymal stem cell storage bag which is taken out or stored by the staff according to the needs is correspondingly taken out or stored, then the controller controls the storage box to descend, the flip cover is in contact with the notch of the annular groove, and the flip cover is pushed to rotate to the position close to the storage groove, so that the flip cover is automatically closed, and the mesenchymal stem cell storage bag is prevented from sliding out of the storage groove in the taking and placing process; according to the invention, the flip cover and the pull rope are connected through the torsion spring and matched, so that the flip cover can be automatically opened and closed in the lifting process of the storage box, and the mesenchymal stem cell storage bag is prevented from sliding out of the storage groove in the taking and placing process, so that the structure of the invention is more precise.

Preferably, an arc-shaped slope is arranged at the corner of the notch of the annular groove, which is close to the box body; an annular sealing gasket is arranged at the joint of the sealing cover and the box body; the annular sealing gasket is fixedly connected with the box body; when the box body is in work, the corner of the notch of the annular groove, which is close to the box body, is arc-shaped, and the arc-shaped slope enables the flip cover to have a buffering angle when the flip cover is closed, so that the flip cover is guided, and the phenomenon that the flip cover is directly closed due to excessive friction generated at the corner of the flip cover and the service life of the flip cover is shortened is avoided; when the storage box enters the box body, the sealing cover is in contact with the annular sealing gasket, so that the interior of the storage box is further sealed, and the storage effect of the mesenchymal stem cells is ensured; the corner of the notch of the annular groove, which is close to the box body, is arranged to be arc-shaped, so that the phenomenon that the turnover cover is excessively rubbed with the box body when being closed to shorten the service life is avoided, and meanwhile, the annular sealing gasket is arranged at the connecting part of the sealing cover and the box body, so that the sealing property of the storage box is ensured.

Preferably, a through hole is formed in the groove bottom of the annular groove, which is close to the cylindrical cavity; the through hole is used for communicating the annular groove with the cylindrical cavity; when the storage tank moves upwards, the space between the bottoms of the annular grooves of the storage box is enlarged, and nitrogen in the cylindrical cavity flows into the space between the storage box and the bottoms of the annular grooves from the through holes; the resistance formed by enlarging the space between the storage box and the bottom of the annular groove is avoided, and the storage box is prevented from rising; according to the invention, through holes are uniformly arranged at the groove bottom of the annular groove close to the cylindrical cavity, the problem that the space between the storage box and the groove bottom of the annular groove is enlarged, so that the pressure intensity is reduced to form resistance, and the storage box is prevented from rising, is avoided, and thus the structure of the invention is more precise.

Preferably, a first bevel gear is fixedly connected to the center of the first gear; the protective shell is rotatably connected with a transmission rod; the transmission rod can slide on the inner wall of the protective shell, one end of the transmission rod, which is close to the first bevel gear, extends to the inside of the protective shell and is fixedly connected with the second bevel gear, and one end of the transmission rod, which is far away from the second bevel gear, extends to the outside of the protective shell and is fixedly connected with a handle; the sliding of the transmission rod can enable the first bevel gear and the second bevel gear to be meshed; when the storage box is in work, a worker holds the handle to push the handle into the protective shell, so that the first bevel gear is meshed with the second bevel gear, the worker rotates the handle, the first bevel gear rotates to drive the first gear to rotate, the second gear rotates, the threaded rod rotates to drive the storage box to ascend, and the storage box reversely rotates in the same way to descend; after the storage box is reset, the handle is pulled out to a position far away from the protective shell, so that the first bevel gear and the second bevel gear are not meshed any more; according to the invention, the mesh of the first bevel gear and the second bevel gear controlled by the handle is matched with the rotation of the handle control transmission rod, so that the aim of manually controlling the lifting of the storage box is realized, and the condition that mesenchymal stem cells cannot be taken out or stored under the condition that a motor is damaged or the power is cut off is avoided, so that an emergency scheme is made for the invention, and the invention has higher practicability.

The invention has the beneficial effects that:

1. according to the invention, the storage temperature of the mesenchymal stem cells is controlled by the temperature control unit and the threaded rod is controlled by the driving unit so as to control the lifting of the storage box to be matched, so that the mesenchymal stem cell storage bag can be taken by a worker more conveniently, the time for taking the mesenchymal stem cells is saved, meanwhile, the temperature control unit is arranged in the storage box so that the cold energy is not easy to lose, the utilization rate of a cold source is improved, the use of the refrigerator is more economical, and the practicability of the refrigerator is further improved.

2. According to the invention, the cylindrical cavity is cooled by the nitrogen entering the second air inlet pipe, and the temperature in the cylindrical cavity is regulated and controlled by the annular pipeline together with the oxygen entering the first air inlet pipe, so that the mesenchymal stem cells are frozen, and meanwhile, the temperature can be controlled to be increased or decreased by controlling the flow rate of the nitrogen or the flow rate of the oxygen when the temperature needs to be regulated, so that the practicability of the invention is improved, and the operation of workers is facilitated.

3. According to the invention, liquid nitrogen enters the second air inlet pipe 14 to cool the cylindrical cavity 18, and then the liquid nitrogen and oxygen enter the first air inlet pipe 13 to regulate and control the temperature in the cavity through the annular pipeline 17, so that the mesenchymal stem cells are frozen, and the freezing temperature of the mesenchymal stem cells is ensured, thereby improving the practicability of the invention and facilitating the operation of workers.

Drawings

This is further explained below with reference to the drawings.

FIG. 1 is a perspective view of the present invention;

FIG. 2 is a cross-sectional view of the present invention;

FIG. 3 is an enlarged view of A in FIG. 2;

FIG. 4 is a perspective view of the storage box of the present invention;

FIG. 5 is a sectional view of the storage box of the present invention;

FIG. 6 is an enlarged view of B in FIG. 5;

in the figure: 1. a box body; 11. a storage box; 111. a storage tank; 112. an annular groove; 12. a threaded rod; 13. a first air inlet pipe; 14. a second air inlet pipe; 15. a first air outlet pipe; 16. a second air outlet pipe; 17. an annular duct; 18. a cylindrical cavity; 2. a protective shell; 21. a motor; 22. a second gear; 23. a first bevel gear; 24. a second bevel gear; 25. a transmission rod; 26. a handle; 27. a first gear; 3. an annular seal; 4. an arc-shaped slope; 5. a vacuum layer; 6. a through hole; 7. a sealing cover; 8. a cover is turned; 81. pulling a rope; 82. a torsion spring.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

As shown in fig. 1 to 6, the mesenchymal stem cell storage box of the present invention comprises a box body 1, a temperature control unit, a driving unit, a threaded rod 12, a storage box 11 and a controller; the box body 1 is cylindrical, and a cylindrical cavity 18 is arranged in the box body 1; a temperature control unit is arranged in the cylindrical cavity 18; the upper surface of the box body 1 is provided with an annular groove 112; the annular groove 112 surrounds the cylindrical cavity 18, and the storage box 11 is connected in the annular groove 112 in a sliding mode; a sealing cover 7 is arranged above the storage box 11, and two threaded rods 12 are connected with the storage box 11 through internal threads; the other ends of the two threaded rods 12 penetrate through the box body 1 and extend to the outside of the box body 1 to be fixedly connected with a first gear 27, the two threaded rods 12 are in rotary sealing connection with the box body 1, and a driving unit is arranged between the two threaded rods 12; the drive unit can control the rotation of the two threaded rods 12; a protective shell 2 is fixedly connected below the box body 1; the protective shell 2 wraps the other end of the threaded rod 12 and the driving unit; the controller is used for controlling the automatic operation of the mesenchymal stem cell storage box;

when the mesenchymal stem cell freezing storage method works, the mesenchymal stem cell storage bag needs to be placed in a freezing tank for freezing storage in the mesenchymal stem cell freezing storage process, and a temperature control unit in the prior art is positioned outside a mesenchymal stem cell storage box, so that the cold source utilization rate of the storage method is low, and energy waste can be caused; meanwhile, when the refrigerator is used, the refrigerator needs to be turned over, so that time and labor are wasted, and the time loss of workers is caused;

therefore, when the storage box is used by a worker, the worker controls the temperature control unit through the controller to enable the temperature in the storage chamber to meet the storage requirement of the mesenchymal stem cells, the controller controls the driving unit to rotate the threaded rod 12 to lift the storage box 11, the worker puts the storage box 11 into a mesenchymal stem cell storage bag, and at the moment, the controller controls the driving unit to reversely rotate the threaded rod 12 to enable the storage box 11 to descend and cover the sealing cover 7 through manual operation to ensure sealing; when the staff needs to take out the mesenchymal stem cell storage bag again, the staff controls the driving unit to drive the threaded rod 12 to rotate by using the controller, so that the storage box 11 is lifted; the temperature control unit is positioned in the storage box 11, and cold energy is transmitted to the outside, so that compared with the prior art that the temperature control unit is arranged outside the storage box 11, the cold source utilization rate is higher, and the energy utilization is more sufficient;

according to the invention, the storage temperature of the mesenchymal stem cells is controlled by the temperature control unit and the lifting of the storage box 11 is controlled by controlling the threaded rod 12 by the driving unit, so that the mesenchymal stem cell storage bag can be taken by a worker more conveniently, the time for taking the mesenchymal stem cells is saved, meanwhile, the temperature control unit is arranged in the storage box 11, so that the cold energy is not easy to lose, the utilization rate of a cold source is improved, the use of the refrigerator is more economic, and the practicability of the refrigerator is further improved.

As an embodiment of the present invention, the temperature control unit includes an annular pipe 17, a first air inlet pipe 13, a second air inlet pipe 14, a first air outlet pipe 15, and a second air outlet pipe 16; the upper surface of the box body 1 is fixedly connected with a first air inlet pipe 13, a second air inlet pipe 14, a second air outlet pipe 16 and a first air outlet pipe 15; the first air inlet pipe 13, the second air inlet pipe 14, the second air outlet pipe 16 and the first air outlet pipe 15 penetrate through the box body 1 and are communicated with the cylindrical cavity 18; an annular pipeline 17 is fixedly connected between the first air inlet pipe 13 and the first air outlet pipe 15; the annular duct 17 is located inside a cylindrical cavity 18;

when the device works, a worker controls the second air outlet pipe 16 to discharge air to discharge the air in the cylindrical cavity 18 by using the controller, then slowly introduces liquid nitrogen into the second air inlet pipe 14, and simultaneously controls the first air inlet pipe 13 to introduce oxygen into the annular pipeline 17 to discharge air from the first air outlet pipe 15 to form oxygen circulation, the liquid nitrogen generates physical liquefaction reaction in the cylindrical cavity 18 to absorb heat and realize the cooling function, the arrangement of the oxygen annular pipeline 17 can enable cold air to be in contact with the surface of the annular pipeline 17 to realize temperature rise of the air in the cylindrical cavity 18, so that the temperature difference between the inside of the annular pipeline 17 and the inside of the cylindrical cavity 18 can be controlled by controlling the flow rate of the oxygen in the annular pipeline 17, the temperature in the cylindrical cavity 18 can be regulated, the application range of the storage box 11 is improved, meanwhile, the arrangement of the annular pipeline 17 enables the surface of the annular pipeline 17 to be in uniform contact with the cold air in the cylindrical cavity 18 when the temperature is regulated, so that the temperature control of the circular duct 17 is synchronized in every direction, and the temperature transferred to the storage box 11 by the cylindrical cavity 18 is uniform; according to the invention, liquid nitrogen enters the second air inlet pipe 14 to cool the cylindrical cavity 18, and then the liquid nitrogen and oxygen enter the first air inlet pipe 13 to regulate and control the temperature in the cavity through the annular pipeline 17, so that the mesenchymal stem cells are frozen, and the freezing temperature of the mesenchymal stem cells is ensured, thereby improving the practicability of the invention and facilitating the operation of workers.

As an embodiment of the present invention, the driving unit includes a motor 21, a first gear 27, and a second gear 22; the motor 21 is fixedly connected with the lower surface of the box body 1, and the output end of the motor 21 is fixedly connected with a first gear 27; the first gear 27 is meshed with the second gear 22; the two second gears 22 are fixedly connected with one ends of the two threaded rods 12 far away from the box body 1 respectively;

when the device works, in the process of needing to take the mesenchymal stem cells, a worker controls the motor 21 to rotate by using the controller, the motor 21 rotates to drive the first gear 27 to rotate, the first gear 27 rotates to drive the second gears 22 to rotate, so that the two threaded rods 12 are driven to rotate, the threaded rods 12 rotate to enable the storage box 11 to ascend, when the storage box 11 ascends to a position where the worker can take out the mesenchymal stem cells, the controller controls the motor 21 to stop rotating, at the moment, the worker takes out the mesenchymal stem cells needing to be used, and controls the motor 21 to reversely rotate by using the controller to enable the storage box 11 to move downwards until the storage box 11 returns to the box body 1, the controller controls the motor 21 to be closed, and the storage box 11 stops moving; according to the invention, the first gear 27 drives the second gear 22 to rotate, so that the threaded rod 12 is driven to rotate, the storage box 11 can be automatically lifted by controlling the motor 21 through the controller, convenience is provided for taking and placing mesenchymal stem cells by workers, and the invention has more rationality.

As an embodiment of the present invention, a vacuum layer 5 is provided in the box body 1; the vacuum layer 5 wraps the annular groove 112 and the cylindrical cavity 18; when the refrigerator works, the temperature in the storage box 11 is transferred by the temperature control unit in the cylindrical cavity 18, and the vacuum layer 5 is arranged between the annular groove 112 and the box body 1 and between the cylindrical cavity 18 and the box body 1, so that the temperature in the cylindrical cavity 18 is reduced from being transferred to the outside, the utilization rate of cold energy is increased, the energy waste is reduced, and the refrigerator has practical application effect.

As an embodiment of the present invention, the storage box 11 has a shape of a torus; the outer wall of the storage box 11 is uniformly provided with storage grooves 111, and the part of the storage box 11 connected with the threaded rod 12 is solid; when the device works, the connecting part of the storage box 11 and the threaded rod 12 is solid, so that the storage of the mesenchymal stem cells is not influenced by the rotation of the threaded rod 12; meanwhile, the storage box 11 stores the mesenchymal stem cells, and the mesenchymal stem cell storage bags with different numbers can be respectively placed in different storage tanks 111, so that the mesenchymal stem cell storage bags with specific numbers can be conveniently searched and taken out; according to the invention, the storage and the taking out of the mesenchymal stem cell storage bag are facilitated through the arrangement of the storage groove 111, so that a worker can reasonably store the mesenchymal stem cell storage bag and quickly take out the mesenchymal stem cell storage bag, the working efficiency of the worker is improved, and convenience is brought to the worker.

As an embodiment of the present invention, the upper surface of the storage box 11 is fixedly connected to the sealing cover 7; when the storage box is in work, a worker controls the driving unit to rotate the threaded rod 12 through the controller, so that the sealing cover 7 is fixedly connected with the storage box 11 in the downward moving process of the storage box 11, the sealing cover 7 seals the storage box 11 while the storage box 11 moves, the step of manually closing the sealing cover 7 by the worker is omitted, the operation process of the worker is saved, the working efficiency of the worker is further improved, and the storage box has practicability, so that the storage box has the characteristics of convenience in operation and simplicity in working process.

As an embodiment of the present invention, the storage groove 111 is hinged to the flip cover 8 near the outer edge of the protective casing 2 through a torsion spring; a pull rope 81 is fixedly connected to one side of the turnover cover 8 close to the storage groove 111; the other end of the pull rope 81 is fixedly connected with one surface of the storage groove 111 far away from the protective shell 2; in operation, when the storage box 11 moves upwards, the flip 8 on the storage slot 111 is opened under the action of the torsion spring, and the opening angle of the flip 8 is limited by the arrangement of the pull rope 81; the mesenchymal stem cell storage bag taken out or stored by the staff correspondingly takes out or stores as required, then the controller controls the storage box to descend, the flip cover 8 is in contact with the notch of the annular groove 112, the flip cover 8 is pushed to rotate towards the position against the storage groove 111, so that the flip cover 8 is automatically closed, and the mesenchymal stem cell storage bag is prevented from sliding out of the storage groove 111 in the taking and placing process; according to the invention, the flip cover 8 and the pull rope 81 are connected through the torsion spring and matched with each other, so that the flip cover 8 can be automatically opened and closed in the lifting process of the storage box 11, and the mesenchymal stem cell storage bag is prevented from sliding out of the storage groove 111 in the taking and placing process, and the structure of the invention is more precise.

As an embodiment of the invention, the notch of the annular groove 112 is provided with an arc-shaped slope 4 at the corner close to the box body 1; an annular sealing gasket 3 is arranged at the joint of the sealing cover 7 and the box body 1; the annular sealing gasket 3 is fixedly connected with the box body 1; when the box body is in operation, the corner of the notch of the annular groove 112, which is close to the box body 1, is arc-shaped, and the arc-shaped slope 4 enables the flip cover 8 to have a buffering angle when the flip cover 8 is closed, so that the flip cover 8 is guided, and the phenomenon that the flip cover 8 is directly closed due to excessive friction generated at the corner is avoided, so that the service life of the flip cover 8 is shortened; when the storage box 11 enters the box body 1, the sealing cover 7 is in contact with the annular sealing gasket 3, so that the internal sealing of the storage box 11 is further realized, and the storage effect of the mesenchymal stem cells is ensured; the corner of the notch of the annular groove 112 close to the box body 1 is arc-shaped, so that the phenomenon that the service life is shortened due to excessive friction between the turnover cover 8 and the box body 1 when the turnover cover 8 is closed is avoided, and meanwhile, the annular sealing gasket 3 is arranged at the joint of the sealing cover 7 and the box body 1, so that the sealing performance of the storage box 11 is ensured.

As an embodiment of the invention, the annular groove 112 is provided with a through hole 6 close to the bottom of the cylindrical cavity 18; the through hole 6 communicates the annular groove 112 with the cylindrical cavity 18; in operation, when the storage tank 111 moves upwards, the space between the bottoms of the annular grooves 112 of the storage box 11 is enlarged, and nitrogen in the cylindrical cavity 18 flows into the space between the storage box 11 and the bottoms of the annular grooves 112 from the through holes 6; the space between the storage box 11 and the bottom of the annular groove 112 is prevented from being enlarged to form resistance to hinder the storage box 11 from rising; according to the invention, through holes 6 are uniformly arranged at the groove bottom of the annular groove 112 close to the cylindrical cavity 18, so that the problem that the space between the storage box 11 and the groove bottom of the annular groove 112 is enlarged to reduce the pressure to form resistance and block the storage box 11 from rising is avoided, and the structure of the invention is more precise.

As an embodiment of the present invention, a first bevel gear 23 is fixedly connected to the center of the first gear 27; a transmission rod 25 is rotatably connected to the protective shell 2; the transmission rod 25 can slide on the inner wall of the protective shell 2, one end of the transmission rod 25, which is close to the first bevel gear 23, extends to the inside of the protective shell 2 and is fixedly connected with the second bevel gear 24, and one end of the transmission rod 25, which is far away from the second bevel gear 24, extends to the outside of the protective shell 2 and is fixedly connected with the handle 26; the sliding of the transmission rod 25 can enable the first bevel gear 23 and the second bevel gear 24 to be meshed; when the storage box is in work, a worker holds the handle 26 to push the handle 26 into the protective shell 2, so that the first bevel gear 23 is meshed with the second bevel gear 24, the worker rotates the handle 26, the first bevel gear 23 rotates to drive the first gear 27 to rotate, the second gear 22 is driven to rotate, the threaded rod 12 rotates to drive the storage box 11 to ascend, and the storage box 11 reversely rotates in the same way to descend; after the storage box 11 is reset, the handle 26 is drawn out to a position far away from the protective shell 2, so that the first bevel gear 23 and the second bevel gear 24 are not engaged; according to the invention, the engagement of the first bevel gear 23 and the second bevel gear 24 is controlled by the handle 26, and the rotation of the transmission rod 25 is controlled by the handle 26 to be matched, so that the aim of manually controlling the lifting of the storage box 11 is realized, and the phenomenon that mesenchymal stem cells cannot be taken out or stored under the condition that the motor 21 is damaged or is powered off is avoided, so that an emergency scheme is made for the invention, and the invention has higher practicability.

The specific working process is as follows:

when the storage box is used by a worker, the worker controls the temperature control unit through the controller to enable the temperature in the storage chamber to meet the storage requirement of mesenchymal stem cells, the controller controls the driving unit to rotate the threaded rod 12 to lift the storage box 11, the worker puts the storage box 11 into a mesenchymal stem cell storage bag, and at the moment, the controller controls the driving unit to reversely rotate the threaded rod 12 to enable the storage box 11 to descend and cover the sealing cover 7 through manual operation to ensure sealing; when the staff needs to take out the mesenchymal stem cell storage bag again, the staff controls the driving unit to drive the threaded rod 12 to rotate by using the controller, so that the storage box 11 is lifted; the temperature control unit is positioned in the storage box 11, and cold energy is transmitted to the outside, so that compared with the prior art that the temperature control unit is arranged outside the storage box 11, the cold source utilization rate is higher, and the energy utilization is more sufficient; the working personnel control the second air outlet pipe 16 to discharge air to discharge the air in the cylindrical cavity 18 by using the controller, then slowly introduce liquid nitrogen into the second air inlet pipe 14, simultaneously, the working personnel control the first air inlet pipe 13 to introduce oxygen into the annular pipeline 17 to discharge air from the first air outlet pipe 15 to form oxygen circulation, the liquid nitrogen generates physical liquefaction reaction in the cylindrical cavity 18 to absorb heat and realize the cooling function, the arrangement of the oxygen annular pipeline 17 can enable cold air to be in contact with the surface of the annular pipeline 17 to realize the temperature rise of the air in the cylindrical cavity 18, therefore, the temperature difference between the annular pipeline 17 and the cylindrical cavity 18 can be controlled by controlling the flow rate of the oxygen in the annular pipeline 17, thereby regulating and controlling the temperature in the cylindrical cavity 18, the application range of the storage box 11 is improved, meanwhile, the arrangement of the annular pipeline 17 enables the surface of the annular pipeline 17 to be in uniform contact with the cold air in the cylindrical cavity 18 when the temperature is regulated and controlled, so that the temperature control of the circular duct 17 is synchronized in every direction, and the temperature transferred to the storage box 11 by the cylindrical cavity 18 is uniform; when mesenchymal stem cells need to be taken out, a worker controls the motor 21 to rotate by using the controller, the motor 21 rotates to drive the first gear 27 to rotate, the first gear 27 rotates to drive the second gears 22 to rotate, so that the two threaded rods 12 are driven to rotate, the threaded rods 12 rotate to enable the storage box 11 to ascend, when the storage box 11 ascends to a position where the worker can take out the mesenchymal stem cells, the controller controls the motor 21 to stop rotating, at the moment, the worker takes out the mesenchymal stem cells needing to be used, and then the controller controls the motor 21 to reversely rotate, so that the storage box 11 moves downwards until the storage box 11 returns to the box body 1, the controller controls the motor 21 to be closed, and the storage box 11 stops moving; the temperature in the storage box 11 is transferred by the temperature control unit in the cylindrical cavity 18, and the vacuum layer 5 is arranged between the annular groove 112 and the box body 1 and between the cylindrical cavity 18 and the box body 1, so that the temperature in the cylindrical cavity 18 is reduced from being transferred to the outside, the utilization rate of cold energy is increased, the energy waste is reduced, and the invention has practical application effect; the connecting part of the storage box 11 and the threaded rod 12 is solid, so that the storage of the mesenchymal stem cells is not influenced by the rotation of the threaded rod 12; meanwhile, the storage box 11 stores the mesenchymal stem cells, and the mesenchymal stem cell storage bags with different numbers can be respectively placed in different storage tanks 111, so that the mesenchymal stem cell storage bags with specific numbers can be conveniently searched and taken out; the working personnel controls the driving unit to rotate the threaded rod 12 through the controller, so that the sealing cover 7 is fixedly connected with the storage box 11 in the process that the storage box 11 moves downwards, the sealing cover 7 seals the storage box 11 while the storage box 11 moves, and the step that the working personnel manually closes the sealing cover 7 is omitted; during the upward movement of the storage box 11, the flip 8 on the storage slot 111 is opened under the action of the torsion spring, and the arrangement of the pull rope 81 limits the opening angle of the flip 8; the mesenchymal stem cell storage bag taken out or stored by the staff correspondingly takes out or stores as required, then the controller controls the storage box to descend, the flip cover 8 is in contact with the notch of the annular groove 112, the flip cover 8 is pushed to rotate towards the position against the storage groove 111, so that the flip cover 8 is automatically closed, and the mesenchymal stem cell storage bag is prevented from sliding out of the storage groove 111 in the taking and placing process; the corner of the notch of the annular groove 112, which is close to the box body 1, is arc-shaped, and the arc-shaped slope 4 enables the flip cover 8 to have a buffering angle when being closed, so that the flip cover 8 is guided, and the phenomenon that the flip cover 8 is directly closed due to excessive friction generated at the corner so as to shorten the service life of the flip cover 8 is avoided; when the storage box 11 enters the box body 1, the sealing cover 7 is in contact with the annular sealing gasket 3, so that the internal sealing of the storage box 11 is further realized, and the storage effect of the mesenchymal stem cells is ensured; when the storage tank 111 moves upward, the space between the bottoms of the annular grooves 112 of the storage box 11 becomes larger, and the nitrogen gas in the cylindrical cavity 18 flows into the space between the storage box 11 and the bottoms of the annular grooves 112 from the through holes 6; the space between the storage box 11 and the bottom of the annular groove 112 is prevented from being enlarged to form resistance to hinder the storage box 11 from rising; an operator holds the handle 26 and pushes the handle into the protective shell 2, so that the first bevel gear 23 is meshed with the second bevel gear 24, at the moment, the operator rotates the handle 26, the first bevel gear 23 rotates to drive the first gear 27 to rotate, the second gear 22 is driven to rotate, the threaded rod 12 rotates to drive the storage box 11 to ascend, and similarly, the threaded rod rotates reversely, so that the storage box 11 descends; after the storage box 11 is reset, the handle 26 is withdrawn to a position away from the protective case 2 so that the first bevel gear 23 and the second bevel gear 24 are no longer engaged.

The foregoing shows and describes the general principles, principal features and advantages of the invention; it will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.