阅读说明：本技术 一种生物有机肥及其制备方法 (Biological organic fertilizer and preparation method thereof ) 是由 安忠芹 吴佳芮 于 2021-10-12 设计创作，主要内容包括：本发明公开了一种生物有机肥及其制备方法。它包括如下重量份数的组分：灵芝100-150份、玉米30-50份、牡蛎20-40份、灵杆菌制剂2-8份、水200-800份。本发明还公开了上述有机肥的制备方法,灵杆菌驯化方式为物理调节培养温度,利用高低温度变化使菌株增强活性,获得更丰富的次生代谢物。本发明制备的生物有机肥喷洒于植物上,能提升光合作用效能,对水稻,玉米,花生等作物有显著的增产、抗逆效果。(The invention discloses a biological organic fertilizer and a preparation method thereof. The paint comprises the following components in parts by weight: 100-150 parts of ganoderma lucidum, 30-50 parts of corn, 20-40 parts of oyster, 2-8 parts of bacillus prodigiosus preparation and 800 parts of water. The invention also discloses a preparation method of the organic fertilizer, the domestication mode of the bacillus prodigiosus is to physically adjust the culture temperature, and the activity of the strain is enhanced by utilizing the change of high and low temperature, so that richer secondary metabolites are obtained. The biological organic fertilizer prepared by the invention is sprayed on plants, can improve the photosynthesis efficiency, and has obvious yield increase and stress resistance effects on crops such as rice, corn, peanut and the like.)

1. The biological organic fertilizer is characterized by comprising the following components in parts by weight: 100-150 parts of ganoderma lucidum, 30-50 parts of corn, 20-40 parts of oyster, 2-8 parts of bacillus prodigiosus preparation and 800 parts of water.

2. The bio-organic fertilizer as claimed in claim 1, wherein the viable count of the bacillus prodigiosus in the bacillus prodigiosus preparation is 3-8X10⁷cfu/g。

3. The bio-organic fertilizer according to claim 1, wherein said bacillus prodigiosus is serratia marcescensSerratia marcescens NS-17。

4. The preparation method of the bio-organic fertilizer is characterized by comprising the following steps of:

(1) according to the weight portion, 100 portions of ganoderma lucidum, 30 to 50 portions of corn and 20 to 40 portions of oyster are crushed by 40 to 80 meshes and evenly mixed;

(2) adding 800 parts of water at 50-65 ℃, inoculating 2-8 parts of bacillus prodigiosus preparation, and fermenting for 18-32 h;

(3) cooling to 25-35 deg.C, fermenting for 10-20 hr, and filtering to obtain filtrate.

5. The method for preparing the bio-organic fertilizer as claimed in claim 4, wherein the filtering is performed by using a filtering net, and the mesh number of the filtering net is 100-200 meshes.

6. The preparation method of the biological organic fertilizer as claimed in claim 4, wherein the filtrate prepared in the step (3) is diluted by 2-30 times.

7. The use of the bio-organic fertilizer of claim 1 for promoting photosynthesis efficiency of plants.

8. The use of the bio-organic fertilizer of claim 1 for promoting crop yield increase.

9. The use of the bio-organic fertilizer of claim 1 for improving crop stress tolerance.

Technical Field

The invention belongs to the technical field of organic fertilizer preparation, and particularly relates to a biological organic fertilizer and a preparation method thereof.

Background

The Ganoderma has umbrella shape and pileus kidney shape, and is fruiting body of Ganoderma of Polyporaceae. Has effects of invigorating qi, tranquilizing mind, relieving cough and asthma, and can be used for treating vertigo, insomnia, palpitation, short breath, and cough and asthma due to asthenia. Ganoderma contains abundant ganoderan, ergosterol, triterpenes, coumarins, volatile oil, stearic acid, benzoic acid, alkaloid, vitamin B2 and vitamin C; the spore also contains mannitol, trehalose, etc.

The bacillus prodigiosus is also called serratia marcescens, belongs to the eubacteriales, enterobacteriaceae and serratia, and is the most representative genus of enterobacteriaceae. The species is gram-negative bacteria, exists in water, soil and human intestinal tract, the cells are rod-shaped or spherical, and the strains can usually produce red prodigiosin. Neutral lipopolysaccharide containing in its cell wall outer membrane a biological activity of resisting cancer, inhibiting infection, enhancing immunity, etc. is called Ganoderma lucidum polysaccharide.

People often research the medicinal value of lucid ganoderma and lucid ganoderma fermentation products, and at present, no report on preparation of fertilizer by fermenting lucid ganoderma by adopting lucid ganoderma through lucid ganoderma is reported.

Disclosure of Invention

The invention aims to provide a biological organic fertilizer and a preparation method thereof.

The biological organic fertilizer comprises the following components in parts by weight: 100-150 parts of ganoderma lucidum, 30-50 parts of corn, 20-40 parts of oyster, 2-8 parts of bacillus prodigiosus preparation and 800 parts of water.

The number of viable bacteria of the bacillus prodigiosus in the bacillus prodigiosus preparation is 3-8X10⁷cfu/g。

The bacillus prodigiosus is Serratia marcescens NS-17.

A preparation method of a biological organic fertilizer comprises the following steps:

(1) according to the weight portion, 100 portions of ganoderma lucidum, 30 to 50 portions of corn and 20 to 40 portions of oyster are crushed by 40 to 80 meshes and evenly mixed;

(2) adding 800 parts of water at 50-65 ℃, inoculating 2-8 parts of bacillus prodigiosus preparation, and fermenting for 18-32 h;

(3) cooling to 25-35 deg.C, fermenting for 10-20 hr, and filtering to obtain filtrate.

The filtration adopts a filter screen, and the mesh number of the filter screen is 100 meshes and 200 meshes.

And (4) diluting the filtrate prepared in the step (3) by 2-30 times.

The biological organic fertilizer is applied to promoting plants to improve photosynthesis efficiency.

The biological organic fertilizer is applied to promoting crop yield increase.

The biological organic fertilizer is applied to improving crop stress resistance.

The invention has the beneficial effects that: the strain is inoculated into a culture medium which takes lucid ganoderma as a main component and fermented to obtain the ganoderin compound microbial polysaccharide, the ganoderin compound microbial polysaccharide contains a treponema conformation, and the main monosaccharide component of the ganoderin compound polysaccharide is analyzed by GC-MS to be arabinose and a small amount of galactose, mannose, xylose and idose; the polysaccharide is mainly in a (4 → 7) glycosidic bond connection configuration and is accompanied by a small number of structures connected by branched chains at the 1 → 6 position, the ganoderin microbial compound polysaccharide is glucan connected by arabinose units through beta- (4 → 7) glycosidic bonds, and the main configuration characteristic is a (4 → 7) beta-D-linear connection framework structure. The domestication mode is to physically adjust the culture temperature, and the activity of the strain is enhanced by utilizing the change of high and low temperature, so as to obtain richer secondary metabolites. The biological organic fertilizer prepared by the invention is sprayed on plants, can improve the photosynthesis efficiency, and has obvious yield increase and stress resistance effects on crops such as rice, corn, peanut and the like.

Detailed Description

In order that the invention may be more fully understood, reference will now be made to the following description. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

In the following examples, the Bacillus species is Serratia marcescens NS-17, which is purchased from Shanghai Haosheng Biotech Co., Ltd, and the viable count of Bacillus is 5X10 in the cultured Bacillus preparation⁷cfu/g。

Example 1

The biological organic fertilizer comprises the following components in parts by weight: 120 parts of lucid ganoderma, 40 parts of corn, 30 parts of oyster, 5 parts of bacillus prodigiosus and 500 parts of water.

The preparation method of the biological organic fertilizer comprises the following steps:

(1) according to the weight parts, 120 parts of lucid ganoderma, 40 parts of corn and 30 parts of oyster are crushed by 60 meshes and uniformly mixed;

(2) adding 500 parts of water with the temperature of 60 ℃, inoculating 5 parts of bacillus prodigiosus preparation, and fermenting for 24 hours;

(3) cooling to 30 deg.C, fermenting for 15h, and filtering with 150 mesh filter screen to obtain filtrate.

Example 2

The biological organic fertilizer comprises the following components in parts by weight: 100 parts of lucid ganoderma, 30 parts of corn, 20 parts of oyster, 3 parts of bacillus prodigiosus and 300 parts of water.

The preparation method of the biological organic fertilizer comprises the following steps:

(1) according to the weight parts, 100 parts of lucid ganoderma, 30 parts of corn and 20 parts of oyster are crushed by 50 meshes and uniformly mixed;

(2) adding 300 parts of water with the temperature of 50 ℃, inoculating 3 parts of bacillus prodigiosus preparation, and fermenting for 32 hours;

(3) cooling to 25 deg.C, fermenting for 18h, and filtering with 120 mesh filter screen to obtain filtrate.

Example 3

The biological organic fertilizer comprises the following components in parts by weight: 150 parts of lucid ganoderma, 50 parts of corn, 40 parts of oyster, 7 parts of bacillus prodigiosus and 700 parts of water.

The preparation method of the biological organic fertilizer comprises the following steps:

(1) according to the weight parts, 150 parts of lucid ganoderma, 50 parts of corn and 40 parts of oyster are crushed by 70 meshes and uniformly mixed;

(2) adding 700 parts of water with the temperature of 65 ℃, inoculating 7 parts of bacillus prodigiosus preparation, and fermenting for 18 hours;

(3) cooling to 35 deg.C, fermenting for 12h, and filtering with 200 mesh filter screen to obtain filtrate.

Comparative example 1

The biological organic fertilizer comprises the following components in parts by weight: 120 parts of lucid ganoderma, 40 parts of corn, 30 parts of oyster, 5 parts of bacillus prodigiosus and 500 parts of water.

The preparation method of the biological organic fertilizer comprises the following steps:

(1) according to the weight parts, 120 parts of lucid ganoderma, 40 parts of corn and 30 parts of oyster are crushed by 60 meshes and uniformly mixed;

(2) adding 500 parts of water with the temperature of 60 ℃, inoculating 5 parts of bacillus prodigiosus preparation, and fermenting for 24 hours;

(3) cooling to 30 deg.C, fermenting for 15h, and filtering with 150 mesh filter screen to obtain filtrate.

Comparative example 1

The biological organic fertilizer comprises the following components in parts by weight: 150 parts of lucid ganoderma, 40 parts of corn, 5 parts of bacillus prodigiosus preparation and 500 parts of water.

The preparation method of the biological organic fertilizer comprises the following steps:

(1) according to the weight parts, 150 parts of lucid ganoderma and 40 parts of corn are crushed by 60 meshes and uniformly mixed;

(2) adding 500 parts of water with the temperature of 60 ℃, inoculating 5 parts of bacillus prodigiosus preparation, and fermenting for 24 hours;

(3) cooling to 30 deg.C, fermenting for 15h, and filtering with 150 mesh filter screen to obtain filtrate.

Comparative example 2

The biological organic fertilizer comprises the following components in parts by weight: 40 parts of corn, 150 parts of oyster, 5 parts of bacillus prodigiosus preparation and 500 parts of water.

The preparation method of the biological organic fertilizer comprises the following steps:

(1) according to the weight portion, 40 portions of corn and 150 portions of oyster are crushed by 60 meshes and evenly mixed;

(2) adding 500 parts of water with the temperature of 60 ℃, inoculating 5 parts of bacillus prodigiosus preparation, and fermenting for 24 hours;

(3) cooling to 30 deg.C, fermenting for 15h, and filtering with 150 mesh filter screen to obtain filtrate.

Experimental example 1:

the inventor divides 1.8 mu of rice test field with same fertility, sunshine and water source into 18 parts, 15 parts are divided into 5 groups on average, and the organic fertilizer of the invention of the embodiment 1-3 and the comparative example 1-2 is used, and is sprayed on rice leaves after water is diluted by 8 times in the rice filling period, and is sprayed once every 5 days; 3 parts of the fertilizer is used as a control group, clear water is sprayed on rice leaves once every 5 days, the same planting method is used during the growth period of crops until the crops are mature, the yield per minute is counted and converted into the yield per mu, and the experimental result is as follows:

TABLE 1

Note: represents P <0.05 compared to control group; # represents P <0.05 compared to example 1.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.