阅读说明：本技术 一种功能性诱食肽饲料添加剂及其制备方法 (Functional food calling peptide feed additive and preparation method thereof ) 是由 张自力 于 2021-09-29 设计创作，主要内容包括：本发明公开了一种功能性诱食肽饲料添加剂及其制备方法,包含以下重要组分：核苷酸和谷氨酸钠；包含以下百分比的重要组分：核苷酸2％-30％,谷氨酸钠5％-70％,氨基酸5％-30％,盐酸吡哆醇0.5％-10％,植物提取物10％-30％,载体酵母抽提物5％-12％；根据配方组合植物原料；将所需原料按比例需要配比后粉碎；用20目筛片进行筛选,将筛选出的原料放入混合机内进行混合；将混合均匀的原料装袋备用；按照所需要的比例数量进行加工提取工艺；本发明包括N甲基-D天冬氨酸与谷氨酸钠配伍核苷酸外源诱食,结合内源调控诱食结合,达到愉悦采食记忆,主要是配伍增效,发挥增效剂盐酸吡哆醇的药理作用。(The invention discloses a functional food calling peptide feed additive and a preparation method thereof, wherein the functional food calling peptide feed additive comprises the following important components: nucleotides and sodium glutamate; comprises the following important components in percentage: 2 to 30 percent of nucleotide, 5 to 70 percent of sodium glutamate, 5 to 30 percent of amino acid, 0.5 to 10 percent of pyridoxine hydrochloride, 10 to 30 percent of plant extract and 5 to 12 percent of carrier yeast extract; combining plant materials according to a formula; the required raw materials are proportioned according to the proportion and then crushed; screening with 20 mesh sieve, and mixing the screened materials in a mixer; bagging the uniformly mixed raw materials for later use; processing and extracting according to the required proportion quantity; the invention comprises the steps of carrying out exogenous feeding attraction by combining N methyl-D aspartic acid and sodium glutamate with compatible nucleotide, combining with endogenous regulation and feeding attraction, achieving joyful feeding memory, mainly carrying out compatibility and synergism, and playing the pharmacological action of a synergist pyridoxine hydrochloride.)

1. A functional food calling peptide feed additive is characterized in that: comprises the following important components: nucleotide and sodium glutamate.

2. The functional phagostimulant peptide feed additive of claim 1, wherein the functional phagostimulant peptide feed additive comprises: comprises the following important components in percentage: 2 to 30 percent of nucleotide, 5 to 70 percent of sodium glutamate, 5 to 30 percent of amino acid, 0.5 to 10 percent of pyridoxine hydrochloride, 10 to 30 percent of plant extract and 5 to 12 percent of carrier yeast extract.

3. The functional phagostimulant peptide feed additive of claim 1, wherein the functional phagostimulant peptide feed additive comprises: also comprises the following important components in percentage: 2 to 30 percent of nucleotide, 5 to 50 percent of sodium glutamate, 5 to 20 percent of amino acid, 0.5 to 10 percent of pyridoxine hydrochloride, 10 to 30 percent of plant extract and 5 to 12 percent of carrier yeast extract.

4. The functional phagostimulant peptide feed additive of claim 3, wherein the functional phagostimulant peptide feed additive comprises the following components: the nucleotide comprises 5 '-disodium adenylate, 5' -disodium guanylate, 5 '-disodium inosinate, disodium cytidylate and 5' -disodium guanylate, and 2-30% of the nucleotide comprises two or more of the above.

5. The functional phagostimulant peptide feed additive of claims 1-4, wherein the functional phagostimulant peptide feed additive comprises: comprises the following important components in percentage: 5-75% of sodium glutamate and 2-20% of nucleotide, wherein 1-10% of disodium 5 '-inosinate and 1-10% of disodium 5' -guanylate.

6. A functional phagostimulant peptide feed additive according to claims 1 to 3 wherein: the amino acid comprises N-methyl-D aspartic acid, D-aspartic acid and aspartic acid, and 5-30% of the amino acid comprises one or more than one of the amino acids.

7. A functional phagostimulant peptide feed additive according to claims 1 to 3 wherein: the plant extract comprises Phasiana vulgaris, Adiantum capillus-veneris, Ceratopteris glabra, Achillea Wilsoniana, Cyrtomium fortunei, Cyperus rotundus, cortex Phellodendri, radix Paeoniae, radix Acanthopanacis Senticosi, cortex et radix Polygalae, rhizoma Acori Graminei, and fructus Aurantii Immaturus; the plant extract 10-30% comprises two or more of the above.

8. A preparation method of a functional food calling peptide feed additive is characterized by comprising the following steps: the preparation method is as follows: .

Combining plant material according to the formulations of claims 2 and 3 in step 1;

step 2, crushing the required raw materials according to the proportion requirement;

step 3, screening by using 20-mesh sieve sheets, and mixing the screened raw materials in a mixer;

step 4, bagging the uniformly mixed raw materials for later use;

and 5, processing and extracting according to the required proportion.

9. The method for preparing the functional food calling peptide feed additive according to claim 8, wherein the method comprises the following steps: the extraction process flow in step 5 is as follows: extracting raw materials with ethanol, concentrating, leaching, extracting with solvent, concentrating, extracting with extractum, spray drying, pulverizing, and packaging to obtain high-concentration extract product containing effective components.

Technical Field

The invention relates to the technical field of animal feed additives, in particular to a functional food calling peptide feed additive and a preparation method thereof.

Background

The animal feeding is a complex process and is influenced by various factors, but the decisive role is the regulation and control of the central nervous system, and the saturated nerve center and the hungry nerve center exist in the central nervous system to regulate the appetite of the animal so as to control the feeding amount of the animal. The nutrition and water of the food are continuously consumed in the body so as to meet the requirements of maintaining basal metabolism, growth and reproduction, stress and immune disease resistance of animals. The smooth muscle of the digestive canal of the animal can be greatly stretched to adapt to the actual requirement.

The stomach can hold more food than the original volume, but the trace amount of acetylcholine can cause the food to contract, and the tension and the physical factors cause negative feedback of the gastrointestinal tract and the digestive tract to determine the important factors of the food intake. The animal can feed more feed to improve the growth performance and develop the maximum growth potential of the animal by the regulation and control of the feed functional additive. In the traditional method, a phagostimulant and a flavoring agent are added into animal feed, but after the animals improve the ingestion, the digestion problem after the animals eat more food cannot be solved, the maintenance time is short, and the ingestion fatigue occurs; the existing phagostimulant takes sodium glutamate as a main formula product, and after animals eat feed containing the phagostimulant, the phenomena of excitability symptom, no silence, mutual putting and nervous symptom occur. The continuous feed intake can not be carried out after the feed is continuously fed, mainly solves the digestion problem after the feed intake is carried out for a plurality of times, causes dyspepsia and diarrhea, and the reasons for the phenomenon are that the amount of glutamic acid is too large, the glutamic acid is an important transmitter of central nerves in the animal body, is easy to generate neurotoxicity, the glutamic acid is easy to accumulate and poison, generates a new stress source, and the dyspepsia causes diarrhea and diarrhea; the prior art uses the raw materials added with the alkaloid compound bacteriostatic additive for preventing and treating diarrhea, reduces stress diarrhea, improves the activity of endogenous digestive enzymes, has certain improvement feeding effect in a short test period, has insufficient consumption of pyridoxine coenzyme in an animal body after a long time, cannot meet the requirement of the body that the coenzyme is converted by decarboxylation of glutamic acid, the glutamic acid is not converted into GABA, slowly causes accumulation and poisoning of the glutamic acid, and does not fundamentally solve the technical problem.

Therefore, the functional food attractant peptide feed additive which can continuously and stably feed, relieve stress, solve the problem of animal digestion feed, convert and utilize and fundamentally achieve the purpose of improving the feed intake becomes a problem to be solved urgently.

Disclosure of Invention

The technical problem to be solved by the invention is that the prior traditional method is to add a phagostimulant and a flavoring agent into animal feed, but after the animals improve the ingestion, the digestion problem after the animals eat more foods cannot be solved, the maintenance time is short, and the ingestion fatigue occurs; the existing phagostimulant takes sodium glutamate as a main formula product, and after animals eat feed containing the phagostimulant, the phenomena of excitability symptom, no silence, mutual putting and nervous symptom occur. The continuous feed intake can not be carried out after the feed is continuously fed, mainly solves the digestion problem after the feed intake is carried out for a plurality of times, causes dyspepsia and diarrhea, and the reasons for the phenomenon are that the amount of glutamic acid is too large, the glutamic acid is an important transmitter of central nerves in the animal body, is easy to generate neurotoxicity, the glutamic acid is easy to accumulate and poison, generates a new stress source, and the dyspepsia causes diarrhea and diarrhea; the prior art uses the raw materials added with the alkaloid compound bacteriostatic additive for preventing and treating diarrhea, reduces stress diarrhea, improves the activity of endogenous digestive enzymes, has certain improvement feeding effect in a short test period, has insufficient consumption of pyridoxine coenzyme in an animal body after a long time, cannot meet the requirement of the body that the coenzyme is converted by decarboxylation of glutamic acid, the glutamic acid is not converted into GABA, slowly causes accumulation and poisoning of the glutamic acid, and does not fundamentally solve the technical problem.

In order to solve the technical problems, the technical scheme provided by the invention is as follows: a functional food calling peptide feed additive comprises the following important components: nucleotide and sodium glutamate.

Further, the following percentages of important components are contained: 2 to 30 percent of nucleotide, 5 to 70 percent of sodium glutamate, 5 to 30 percent of amino acid, 0.5 to 10 percent of pyridoxine hydrochloride, 10 to 30 percent of plant extract and 5 to 12 percent of carrier yeast extract.

Further, the composition also comprises the following important components in percentage: 2 to 30 percent of nucleotide, 5 to 50 percent of sodium glutamate, 5 to 20 percent of amino acid, 0.5 to 10 percent of pyridoxine hydrochloride, 10 to 30 percent of plant extract and 5 to 12 percent of carrier yeast extract.

Further, the nucleotide comprises disodium 5 '-adenylate, disodium 5' -guanylate, disodium 5 '-inosinate, disodium cytidylate and disodium 5' -guanylate, and 2-30% of the nucleotide comprises two or more of the disodium 5 '-adenylate, the disodium 5' -guanylate and the disodium cytidylate.

Further, the composition also comprises the following important components in percentage: 5-75% of sodium glutamate and 2-20% of nucleotide, wherein 1-10% of disodium 5 '-inosinate and 1-10% of disodium 5' -guanylate.

Further, the amino acid comprises N-methyl-D aspartic acid, D-aspartic acid and aspartic acid, and 5-30% of the amino acid comprises one or more of the amino acids.

Further, the plant extract comprises pheasant tail syncope, adiantum capillus-veneris, ophiophyta glabrata, fern, inula vifolia, inula japonicas, aconitum kusnezoffii, adiantum syncope, cibot rhizome, cyrtomium fortunei, ciliate desert-grass, huperzia serrata, phellodendron, peony, acanthopanax, polygala tenuifolia, lycopodium clavatum, and immature bitter orange; the plant extract 10-30% comprises two or more of the above.

A preparation method of a functional food calling peptide feed additive comprises the following steps: .

Combining plant material according to the formulations of claims 2 and 3 in step 1;

step 2, crushing the required raw materials according to the proportion requirement;

step 3, screening by using 20-mesh sieve sheets, and mixing the screened raw materials in a mixer;

step 4, bagging the uniformly mixed raw materials for later use;

and 5, processing and extracting according to the required proportion.

Further, the extraction process flow in step 5 is as follows: extracting raw materials with ethanol, concentrating, leaching, extracting with solvent, concentrating, extracting with extractum, spray drying, pulverizing, and packaging to obtain high-concentration extract product containing effective components.

Compared with the prior art, the invention has the advantages that: the invention innovatively solves the technical problem of accumulation and poisoning of glutamic acid side effects; the invention combines the endogenous regulation and the exogenous food calling according to the central nervous and endogenous physiological regulation of animal feeding; the feeding center is regulated and controlled through stimulation, the transmission of satiety signals is delayed, the intragastric volume reflex is reduced, the digestion, absorption and utilization are facilitated, the secretion of endogenous hormones is regulated, the feed intake and the digestion conversion rate of animals are improved, and the growth performance is improved; the novel nutritional functional feed additive product has the functions of promoting food intake, promoting digestion, resisting stress, enhancing immunity, preventing and treating stress-induced diseases, promoting the rapid growth and weight increment of animals and multiple functions; the palatability is changed from exogenous seasoning, and the endogenous additive acts after the feed is eaten, so that the ingestion center is regulated and controlled, and the memory effect is formed; the various active ingredients promote gastrointestinal peristalsis, secrete digestive juice and help digestion; on the basis of the conventional food intake, the stomach is enlarged, various contractions of the smooth muscle of the digestive tract occur on the basis of the tonicity, the smooth muscle of the digestive tract can be greatly stretched to adapt to the actual demand, and the stomach can contain food with a volume larger than the original volume. Promoting the rapid growth of animals, if the growth speed is increased to meet the basic maintenance requirement, the feed which is fed more is used for growth, and the feed intake of the animals is improved, which is an effective method; the problem of shortage of industrial feed resources is difficult to solve in a short time, unconventional raw materials which are poor in texture and palatability and are not favored by animals to eat can be developed into feed raw material resources by adjusting the eating behaviors of the animals, and the research on the regulation and control of the feed intake of the animals is helpful for realizing the goal; the feed formula cost is reduced, the feed product quality is improved, the compatibility and synergism are verified, the core components play a role in stimulating the feeding center, the satiety signal transmission is controlled and delayed endogenously, the synergistic effect generates a lasting food calling effect, the food consumption is improved, and the weight gain purpose is achieved. The palatability is improved by exogenous seasoning in the feed, sodium glutamate (Glu) is an essential non-essential amino acid for nitrogen metabolism in organisms, and glutamic acid is a component formed by protein, namely one of structural amino acids of protein and peptide; however, when the glutamic acid is added into the feed, the glutamic acid is accumulated in the brain and stimulates the cerebral cortex due to in vivo metabolic disturbance when the glutamic acid is excessively accumulated, so that the excitability is strong, a stress source is generated, and the influence of the stress on the immunity is mainly inhibitory; because the activity of the glutamate decarboxylase in a body is insufficient, sufficient (coenzyme) pyridoxine hydrochloride needs to be supplemented by external aid, the glutamate decarboxylase is activated to catalyze the decarboxylation of the glutamate and then convert the glutamate into gamma-aminobutyric acid (GABA), and the sufficient pyridoxine hydrochloride is needed to assist the metabolism of the glutamate and generate the GABA, so that the activity of the GABA is improved; the invention innovatively solves the problem of side effect accumulation poisoning of glutamic acid. Compounding and combining the composite plant extracts; the effects of inhibiting cholinesterase activity, enhancing the cholinesterase activity in a body and accelerating the hydrolysis of acetylcholine so as to cause neurotransmitter conduction disorder, delay gastrointestinal motility, reduction of digestive juice secretion, dyspepsia and reduction of feed intake are achieved; the pharmacological function generated by compounding is exerted to promote the gastrointestinal motility and glandular secretion of animals, help to digest food, enhance the digestion function, increase the feed intake, and simultaneously need to supplement enough pyridoxine hydrochloride, the organism synthesizes gamma-aminobutyric acid (GABA) which is a relative signal for appetite regulation of amino acids with definite action, and the GABAergic neuron of gamma-aminobutyric acid and a receptor thereof have morphological and functional connection with other appetite regulating factors (NPY, GAL and the like); it is also possible to suppress the release of the appetite suppressing factor alpha MSH (melanocyte stimulating hormone) and thereby increase the intensity of the reaction of the target site to its released NPY; however, the added exogenous chemical synthesized gamma-aminobutyric acid lacks activity in an organism and cannot achieve lasting and effective effects, mainly has the effects of stress resistance, sedation and the like, and needs the endogenous glutamic acid of the organism to generate and transform active GABA; glutamic acid, N-methyl-D-aspartic acid (NMDA), aspartic acid, serving as excitatory central neurotransmitter, exist in different parts of hypothalamus, can stimulate the feeding behavior of animals, activate receptors in the lateral ventricle of the hypothalamus to stimulate the feeding of the animals, a glutamic acid receptor agonist, N-methyl-D-aspartic acid (NMDA), the receptor of Glu involved in appetite stimulation is selectively positioned in LHA, and the LHA can synthesize part of appetite promoting factors such as neuropeptide Y (NPY), GAL and the like; N-methyl-D-aspartate (NMDA) receptors are present in the presynaptic membrane (excitatory nerve endings), modulating the positive feedback of the receptor modulating the release of excitatory amino acids; aspartic acid acts on the long-term reinforcement (memory process) of neurons, and stimulates and eats the Glu transmitter to generate 'favor', so that the eating behavior of the neurons is changed, and the neurons generate dependence. GABA can inhibit the hyperexcitability of the brain, GABA is an inhibitory nerve conduction substance existing in the brain and spinal cord of an animal, is obtained by the catalytic conversion of Glu, needs to be converted and generated in a long-time process, participates in regulating various advanced functions such as food intake, memory, sleep and the like, GABA enables the animal to be quiet by preventing the hyperexcitability of the brain and relieving the stress capacity, promotes the sleep, reduces consumption, regulates the food intake of the animals and promotes the growth; glutamic acid, N methyl-D aspartic acid (NMDA) and aspartic acid belong to acid taste peptides, and amino acid is connected by a peptide chain to form compound small-molecule bioactive polypeptide, and the peptide has a food calling effect and improves the feed intake and the feed utilization efficiency; the yeast extract is obtained by fermenting and enzymolyzing yeast and molasses serving as raw materials by adopting a bioengineering technology, and carrying out enzymolysis on protein, nucleic acid and the like in yeast cells to obtain a product; the invention innovatively researches the effect of enhancing the digestion function, achieves the purposes of durably and stably improving the ingestion and the conversion utilization rate, and achieves similar pharmacological effects of chemical components in the experimental compound compatible plant extract; mainly takes the 'inhibition of the activity of cholinesterase' which is a conductive neurotransmitter as a key point, and extracts essential components from compatible plant extracts; plant compound components such as flavonoids, saponin glycoside, sterol, polysaccharide, polyphenol, alkene, terpenes, etc.; comprises N kinds of plants of fern family, including fern family, Phasianidae Phasiana, Adiantum capillus-veneris, Pteridium aquilinum, Achillea wilsoniana, Achillea bicolor, Achillea kusnezoffii, Achillea japonica, Cyrtomium fortunei, Cyperus rotundus, Phellodendron, cortex Phellodendri, radix Paeoniae, cortex et radix Polygalae, rhizoma Acori Graminei, and fructus Aurantii Immaturus; preferably, a variety of formulations are contemplated, including all plant extracts that act in a "cholinesterase activity inhibiting" mechanism of action; the fern natural resources are rich, the purification biological process is adopted for enzymolysis and extraction, and the wall-broken plant cells are adopted, so that the advantages of releasing components in the plant cells, high leaching rate, low cost, no need of special equipment, large-scale industrial production and the like are achieved; pharmacological action; the compound has the effects of inhibiting cholinesterase activity, stimulating salivary secretion, promoting digestion of food, and promoting digestion, eating more quickly and growth quickly; acetylcholine affects nerve conduction, and can cause disorders such as slow gastrointestinal motility, decreased digestive juice secretion in the digestive tract, inappetence, dyspepsia, etc.; wherein cortex Phellodendri also has effects of promoting pancreas secretion, and digesting sugar, protein and fat; cortex et radix Polygalae has tranquilizing and hypnotic effects; the plant extract contains various effective compound components, peptides and other traditional Chinese medicine components, and has the functions of activating the secretion of neurotrophic factors, enhancing the memory function, resisting oxidation, eliminating free radicals, regulating blood sugar, regulating the immune function, promoting the growth and the like; the invention has reasonable design and is worth popularizing.

Detailed Description

The following is a further detailed description of a functional phagostimulant peptide feed additive of the invention.

A functional food calling peptide feed additive comprises the following important components: nucleotide and sodium glutamate.

Comprises the following important components in percentage: 2 to 30 percent of nucleotide, 5 to 70 percent of sodium glutamate, 5 to 30 percent of amino acid, 0.5 to 10 percent of pyridoxine hydrochloride, 10 to 30 percent of plant extract and 5 to 12 percent of carrier yeast extract.

Also comprises the following important components in percentage: 2 to 30 percent of nucleotide, 5 to 50 percent of sodium glutamate, 5 to 20 percent of amino acid, 0.5 to 10 percent of pyridoxine hydrochloride, 10 to 30 percent of plant extract and 5 to 12 percent of carrier yeast extract.

The nucleotide comprises 5 '-disodium adenylate, 5' -disodium guanylate, 5 '-disodium inosinate, disodium cytidylate and 5' -disodium guanylate, and 2-30% of the nucleotide comprises two or more of the above.

Also comprises the following important components in percentage: 5-75% of sodium glutamate and 2-20% of nucleotide, wherein 1-10% of disodium 5 '-inosinate and 1-10% of disodium 5' -guanylate.

The amino acid comprises N-methyl-D aspartic acid, D-aspartic acid and aspartic acid, and 5-30% of the amino acid comprises one or more than one of the amino acids.

The plant extract comprises Phasiana vulgaris, Adiantum capillus-veneris, Ceratopteris glabra, Achillea Wilsoniana, Cyrtomium fortunei, Cyperus rotundus, cortex Phellodendri, radix Paeoniae, radix Acanthopanacis Senticosi, cortex et radix Polygalae, rhizoma Acori Graminei, and fructus Aurantii Immaturus; the plant extract 10-30% comprises two or more of the above.

A preparation method of a functional food calling peptide feed additive comprises the following steps:

combining plant material according to the formulations of claims 2 and 3 in step 1;

step 2, crushing the required raw materials according to the proportion requirement;

step 3, screening by using 20-mesh sieve sheets, and mixing the screened raw materials in a mixer;

step 4, bagging the uniformly mixed raw materials for later use;

and 5, processing and extracting according to the required proportion.

The extraction process flow in step 5 is as follows: extracting raw materials with ethanol, concentrating, leaching, extracting with solvent, concentrating, extracting with extractum, spray drying, pulverizing, and packaging to obtain high-concentration extract product containing effective components.

The specific implementation process of the functional food calling peptide feed additive is as follows: examples 1,

Example 1, a functional phagostimulant peptide feed additive, 20% of nucleotide, 30% of sodium glutamate, 15% of amino acid, 5% of pyridoxine hydrochloride, 20% of plant extract and 10% of carrier yeast extract.

Two of nucleotide disodium 5 '-adenylate, disodium 5' -guanylate, disodium 5 '-inosinate, disodium cytidylate and disodium 5' -guanylate are selected, and the specific gravity is 1: 1; the amino acid is N-methyl-D aspartic acid; the plant extracts are: phasianidae Philippinensis, Achillea Wilsoniana Hance, cortex Phellodendri and cortex et radix Polygalae, with a specific gravity of 30:30:20: 20;

a preparation method of a functional food calling peptide feed additive comprises the following steps:

step 1, combining plant raw materials according to a required formula;

step 2, crushing the required raw materials according to the proportion requirement;

step 3, screening by using 20-mesh sieve sheets, and mixing the screened raw materials in a mixer;

step 4, bagging the uniformly mixed raw materials for later use;

and 5, processing and extracting according to the required proportion.

Example 2, a functional phagostimulant peptide feed additive, 20% of nucleotide, 25% of sodium glutamate, 20% of amino acid, 5% of pyridoxine hydrochloride, 20% of plant extract and 10% of carrier yeast extract.

Two of nucleotide disodium 5 '-adenylate, disodium 5' -guanylate, disodium 5 '-inosinate, disodium cytidylate and disodium 5' -guanylate are selected, and the specific gravity is 1: 1; the amino acid is N-methyl-D aspartic acid; the plant extracts are: phasianidae Philippinensis, Achillea Wilsoniana Hance, cortex Phellodendri and cortex et radix Polygalae, with a specific gravity of 30:30:20: 20.

A preparation method of a functional food calling peptide feed additive comprises the following steps:

step 1, combining plant raw materials according to a required formula;

step 2, crushing the required raw materials according to the proportion requirement;

step 3, screening by using 20-mesh sieve sheets, and mixing the screened raw materials in a mixer;

step 4, bagging the uniformly mixed raw materials for later use;

and 5, processing and extracting according to the required proportion.

Example 3, a functional phagostimulant peptide feed additive, nucleotide 15%, sodium glutamate 35%, amino acid 20%, pyridoxine hydrochloride 5%, plant extract 20%, carrier yeast extract 5%.

Two of nucleotide disodium 5 '-adenylate, disodium 5' -guanylate, disodium 5 '-inosinate, disodium cytidylate and disodium 5' -guanylate are selected, and the specific gravity is 1: 1; the amino acid is N-methyl-D aspartic acid; the plant extracts are: phasianidae Philippinensis, Achillea Wilsoniana Hance, cortex Phellodendri and cortex et radix Polygalae, with a specific gravity of 30:30:20: 20.

A preparation method of a functional food calling peptide feed additive comprises the following steps:

step 1, combining plant raw materials according to a required formula;

step 2, crushing the required raw materials according to the proportion requirement;

step 3, screening by using 20-mesh sieve sheets, and mixing the screened raw materials in a mixer;

step 4, bagging the uniformly mixed raw materials for later use;

and 5, processing and extracting according to the required proportion.

Example 4, a functional phagostimulant peptide feed additive, nucleotide 10%, glutamic acid 40%, amino acid 20%, pyridoxine hydrochloride 3%, plant extract 15%, carrier yeast extract 12%.

Two of nucleotide disodium 5 '-adenylate, disodium 5' -guanylate, disodium 5 '-inosinate, disodium cytidylate and disodium 5' -guanylate are selected, and the specific gravity is 1: 1; the amino acid is N-methyl-D aspartic acid; the plant extracts are: phasianidae Philippinensis, Achillea Wilsoniana Hance, cortex Phellodendri and cortex et radix Polygalae, with a specific gravity of 30:30:20: 20.

A preparation method of a functional food calling peptide feed additive comprises the following steps:

step 1, combining plant raw materials according to a required formula;

step 2, crushing the required raw materials according to the proportion requirement;

step 3, screening by using 20-mesh sieve sheets, and mixing the screened raw materials in a mixer;

step 4, bagging the uniformly mixed raw materials for later use;

and 5, processing and extracting according to the required proportion.

The following is a summary of the results of the experimental piglets and the growing pigs and sows at each stage.

Table one; the content of glutamic acid in the total amino acids in the raw materials of the feed formula (unit:%);

from table 7, it can be seen that the conventional raw material classical formula and the unconventional raw material formula of the feed, each raw material contains glutamic acid, the percentage of the glutamic acid contained in the raw material is measured according to the new standard of the NRC-2012 document, the glutamic acid is added exogenously, and meanwhile, sufficient vitamin B6, decarboxylase activity and large-dose exogenous supplement are needed.

Table two; an experimental feed formula;

description is given; the experimental group did not add the sweetener and the flavor, and 150 g of phagostimulant peptide was added per ton. The control group is added with sweetener and flavoring agent, and no phagostimulant peptide additive, and the formula composition is unchanged.

Table three; an experimental feed formula;

description of the drawings: on the basis of a feed formula, 0.1 kg/ton of the product of the invention, namely 'phagostimulant', is added, and the effect is improved compared with the experimental feeding. Using unconventional raw materials; the formula of corn and soybean meal is changed, and the cost is reduced.

Table four; the nutrition index of the experimental product premix feed is unit/kg.

Description is given; the premix products with the contents of 1 percent, 4 percent and 12 percent are added; organic trace elements, amino acid chelates, various amino acids, various vitamins, compound enzymes, phytase, various probiotics, a medicine premix, a compound acidifier, a functional feed additive, organic mineral substances, a protein raw material carrier and the like. )

Experimental example one, experimental subjects and methods:

1.1 subject; after 28 days of weaning, the tripartite hybrid piglet (Dux long x big). Healthy weaned piglets are divided into an experimental group and a control group at random by 180 heads and mothers.

1.2 experiment time; the experimental time was 22 days.

1.3 experimental protocol; a conventional sweetener phagostimulant and flavoring agent product for basic feed is added into the basic feed to be used as a control group, and a nutritional functional phagostimulant peptide feed additive product of the invention is added into the basic feed to be used as an experimental group; (product of example one preparation). The pigs of the same breed were randomly divided into 3 groups. The test pigs were fasted (free drinking) for 12h and then weighed on empty stomach the following morning at the start and end of the test, and the initial and final weights of the test pigs were measured head by head and the feed consumption was counted in duplicate. The average daily gain, average daily feed intake, and feed-to-weight ratio of each group were calculated.

1.4 experiment daily ration; see table 1, formula feed for creep piglets;

1.5 test management; captive breeding; the nursing bed can be used for freely taking food and drinking water, recording fed materials and residual feed, and recording the conditions of appetite, mental state, excrement and the like and other abnormal conditions during the feeding period, and the nursing bed is normally carried out according to the piglet feeding management.

1.6, processing data; and (5) adopting Excel to perform data analysis processing.

2. Analyzing test results; table 3 piglet primary growth performance index;

grouping	Experiment 1	Experiment 2	Control group
				Initial body weight kg	7.38	7.41	7.39
Terminal average body weight kg	14.92	14.91	13.55
				Average daily gain kg	0.343	0.341	0.281
Average daily food intake kg	0.461	0.463	0.401
				Meat ratio of materials	1.35	1.36	1.43

Description is given; from the test results, the initial weight of the combination is high, the diarrhea rate is low, and no obvious difference exists. From the point of view of average terminal body weight, average daily gain and average daily food consumption, the experimental group is better, and the control group is worse. Compared with a control group, the experimental group has the advantages that the daily yield is improved by 15.21%, the daily gain is increased by 21.7%, the feed conversion ratio is reduced, the difference is obvious (P is less than 0.05), and diarrhea has no obvious difference.

Experiment example two, the application effect of the nutritional functional food calling peptide feed additive of the invention to two pairs of piglets is as follows:

1. experimental subjects and methods;

1.1 subject; three-way hybrid piglets (Dux long x big) after 35 days of weaning.

1.2 experiment time; the experimental time was 35 days.

1.3 experimental protocol; the basal feed uses a sweetener phagostimulant product in the market as a control group. The basic feed is added with the nutritional functional food calling peptide feed additive product; (product prepared in Experimental example 7) was used as a control group. Pigs of the same breed were randomly divided into two groups. The average daily gain, average daily feed intake and feed-weight ratio of each group were recorded and calculated.

1.4 experiment daily ration; see table 1, piglet compound feed;

1.5 test management; captive breeding; the nursing bed is used for freely taking food and drinking water, recording fed materials and residual feed, recording the conditions of appetite, mental state, excrement and the like and other abnormal conditions during the feeding period, and the feeding management method is normally carried out according to the piglet feeding management.

1.6, processing data; and (5) adopting Excel to perform data analysis processing.

2. Analyzing test results; table 4 main growth performance indicators of piglets:

group of	Number of heads	Average initial weight/kg	Average end weight/kg	Daily intake/kg	Daily gain/kg	Material weight ratio/kg
							Control group I	110	10.38	27.24	0.766	0.482	1.59：1
Experimental group	115	10.01	30.34	0.894	0.581	1.50：1

As can be seen from the table, the daily yield of the experimental group is increased by 16.7% compared with the control group, the daily gain is increased by 20.5%, the feed conversion ratio is reduced, and the difference is significant (P is less than 0.05). The weight increasing cost is reduced. The experimental group feed added with the nutritional functional food calling peptide feed additive product has obvious effect on the anti-stress capability without diarrhea. The experimental group is quiet and sleepy, and the economic benefit is improved.

The third experimental example and the third nutritional functional food calling peptide feed additive have the following application effects on growing pigs:

1. experimental subjects and methods;

1.1 subject; selecting a ternary hybrid piglet (Dux is long and x is large) with the weight of 40-50 kg.

1.2 experiment time; the experimental time was 30 days.

1.3 experimental protocol; the basal feed uses a sweetener phagostimulant product in the market as a control group, and the basal feed is added with the nutrient 1 functional phagostimulant peptide feed additive product; (product prepared in experimental example three) as experimental group. Pigs of the same breed were randomly divided into two groups. The average daily gain, average daily feed intake, and feed-to-weight ratio were recorded and calculated for each group.

1.4 experiment daily ration; see table 1, growing pig compound feed;

1.5 test management; captive breeding; the nursing bed is used for freely taking food and drinking water, recording fed materials and residual feed, recording the conditions of appetite, mental state, excrement and the like and other abnormal conditions during the feeding period, and the feeding management method is normally carried out according to the piglet feeding management.

1.6, processing data; and (5) adopting Excel to perform data analysis processing.

2. Analyzing test results; table 5 main growth performance indexes of growing pigs:

as can be seen from the table, the daily yield of the experimental group is improved by 15.9% compared with the control group, the daily gain is increased by 21.9%, the feed conversion ratio is reduced, the difference is obvious (P is less than 0.05), and the weight gain cost is reduced. The experimental group feed added with the nutritional functional food calling peptide feed additive product has obvious effect on the anti-stress capability without diarrhea. The experimental group is quiet and sleepy, and the economic benefit is improved.

The fifth experimental example shows that the embodiment of the nutritional functional food calling peptide feed additive has the following application effects on fattening pigs:

1. experimental subjects and methods;

1.1 subject; selecting a ternary hybrid fattening pig with the weight of 60kg-100 kg (Dux is long and x is large).

1.2 experiment time; the experimental time was 30 days.

1.3 experimental protocol; the experimental basic feed is not added with the phagostimulant peptide and is taken as a control group, and the basic group is added with the nutritional functional phagostimulant peptide feed additive product; as experimental group. Pigs of the same breed were randomly divided into two groups. The average daily gain, average daily feed intake, and feed-to-weight ratio were recorded and calculated for each group.

1.4 experiment daily ration; see table 1, growing-finishing pig compound feed;

1.5 test management; captive breeding; the nursing bed is used for freely taking food and drinking water, recording fed materials and residual feed, recording the conditions of appetite, mental state, excrement and the like and other abnormal conditions during the feeding period, and the feeding management method is normally carried out according to the feeding management of the fattening pigs.

1.6, processing data; and (5) adopting Excel to perform data analysis processing.

2. Analyzing test results; table 6 main growth performance indexes of growing-finishing pigs:

group of	Number of heads	Average initial weight/kg	Average end weight/kg	Daily intake/kg	Daily gain/kg	Material weight ratio/kg
							Control group	100	62	87.56	2.4	0.852	2.82:1
Experimental group	100	60	89.76	2.7	0.992	2.72:1

As can be seen from the table, compared with the control group, the daily yield of the experimental group is improved by 12.5%, the daily gain is increased by 16.43%, the material-weight ratio is reduced, the difference is obvious (P is less than 0.05), and the weight gain cost is reduced. The experimental group feed of the nutritional functional food calling peptide feed additive product has obvious effect and improves economic benefit by adding the nutritional functional food calling peptide feed additive product into basic daily ration.

Experiment example four; the invention relates to a nutritional functional feeding promoting peptide feed additive, which is divided into an experimental group and a control group according to an application experiment on a lactating sow; the feed intake improvement of the sows is counted to influence the difference of the birth litter weight, the number of weaned piglets, the weight of weaned litter and the diarrhea rate and the growth performance of the suckling piglets.

1.1 subject; the binary sows with similar gestation times and body conditions are selected to breed 100 sows in total,

1.2 experiment time; the experimental time was 46 days.

1.3 experimental protocol; the experimental basic feed is not added with the phagostimulant peptide and is taken as a control group, and the basic group is added with the nutritional functional phagostimulant peptide feed additive product; as a control group. The sows of the same breed are randomly divided into three groups. And recording and calculating the average feed intake, birth weight, weaning weight and survival rate of each group of sows.

1.4 experiment daily ration; see table 1, sow formula feed;

1.5 test management; adding into basal feed at 21 days before delivery, and weaning until 25 days of lactation. The sow obstetric table can be used for freely taking food and drinking water, the situations of feeding and residual feed, appetite, mental state, excrement and the like and other abnormal situations during the feeding period are recorded, and the feeding management method is normally carried out according to the feeding management of the sow.

1.6 data processing and data analysis processing by using Excel.

The application effect is as follows: three weeks prior to birth to the lactation period of the sow. The addition of the product of the invention into the feed for sows shows that; the lactation yield of the sows and the weaning weight of the piglets are improved, the feed intake of the sows is increased, the milk yield is increased, the feed intake of the sows per day is 1.8kg/+0.45 kg/piglet/day, and 11 piglets are on average. Particularly under the condition of heat stress, the feed intake of the sow is obviously reduced, and the problem can be solved by using the nutritional functional feeding promoting peptide feed additive product.

Table 6, analysis of test results; influence on the productivity of sows:

the results show; the feed intake of the sow is obviously improved by 13.3 percent, the production performance of the sow is obviously improved, the average weight of piglets is improved by 10.5 percent, the difference is obvious (P is less than 0.05), the weaning survival rate is obviously improved, and the overall economic benefit is improved.

The invention combines the endogenous regulation and the exogenous food calling according to the central nervous and endogenous physiological regulation of animal feeding; the feeding center is regulated and controlled through stimulation, the transmission of satiety signals is delayed, the intragastric volume reflex is reduced, the digestion, absorption and utilization are facilitated, the secretion of endogenous hormones is regulated, the feed intake and the digestion conversion rate of animals are improved, and the growth performance is improved; the novel nutritional functional feed additive product has the functions of promoting food intake, promoting digestion, resisting stress, enhancing immunity, preventing and treating stress-induced diseases, promoting the rapid growth and weight increment of animals and multiple functions; the palatability is changed from exogenous seasoning, and the endogenous additive acts after the feed is eaten, so that the ingestion center is regulated and controlled, and the memory effect is formed; the various active ingredients promote gastrointestinal peristalsis, secrete digestive juice and help digestion; on the basis of the conventional food intake, the stomach is enlarged, various contractions of the smooth muscle of the digestive tract occur on the basis of the tonicity, the smooth muscle of the digestive tract can be greatly stretched to adapt to the actual demand, and the stomach can contain food with a volume larger than the original volume. Promoting the rapid growth of animals, if the growth speed is increased to meet the basic maintenance requirement, the feed which is fed more is used for growth, and the feed intake of the animals is improved, which is an effective method; the problem of shortage of industrial feed resources is difficult to solve in a short time, unconventional raw materials which are poor in texture and palatability and are not favored by animals to eat can be developed into feed raw material resources by adjusting the eating behaviors of the animals, and the research on the regulation and control of the feed intake of the animals is helpful for realizing the goal; the feed formula cost is reduced, the feed product quality is improved, the compatibility and synergism are verified, the core components play a role in stimulating the feeding center, the satiety signal transmission is controlled and delayed endogenously, the synergistic effect generates a lasting food calling effect, the food consumption is improved, and the weight gain purpose is achieved. The palatability is improved by exogenous seasoning in the feed, sodium glutamate (Glu) is an essential non-essential amino acid for nitrogen metabolism in organisms, and glutamic acid is a protein constituent, namely one of structural amino acids of protein and peptide; however, when the glutamic acid is added into the feed, the glutamic acid is accumulated in the brain and stimulates the cerebral cortex due to in vivo metabolic disturbance when the glutamic acid is excessively accumulated, so that the excitability is strong, a stress source is generated, and the influence of the stress on the immunity is mainly inhibitory; because the activity of the glutamate decarboxylase in a body is insufficient, sufficient (coenzyme) pyridoxine hydrochloride needs to be supplemented by external aid, the glutamate decarboxylase is activated to catalyze the decarboxylation of the glutamate and then convert the glutamate into gamma-aminobutyric acid (GABA), and the sufficient pyridoxine hydrochloride is needed to assist the metabolism of the glutamate and generate the GABA, so that the activity of the GABA is improved; the effects of inhibiting cholinesterase activity, enhancing the cholinesterase activity in a body and accelerating the hydrolysis of acetylcholine so as to cause neurotransmitter conduction disorder, delay gastrointestinal motility, reduction of digestive juice secretion, dyspepsia and reduction of feed intake are achieved; the pharmacological function generated by compounding is exerted to promote the gastrointestinal motility and glandular secretion of animals, help to digest food, enhance the digestion function, increase the feed intake, and simultaneously need to supplement enough pyridoxine hydrochloride, the organism synthesizes gamma-aminobutyric acid (GABA) which is a relative signal for appetite regulation of amino acids with definite action, and the GABAergic neuron of gamma-aminobutyric acid and a receptor thereof have morphological and functional connection with other appetite regulating factors (NPY, GAL and the like); it is also possible to suppress the release of the appetite suppressing factor alpha MSH (melanocyte stimulating hormone) and thereby increase the intensity of the reaction of the target site to its released NPY; however, the added exogenous chemical synthesized gamma-aminobutyric acid lacks activity in an organism and cannot achieve lasting and effective effects, mainly has the effects of stress resistance, sedation and the like, and needs the endogenous glutamic acid of the organism to generate and transform active GABA; glutamic acid, N-methyl-D-aspartic acid (NMDA), aspartic acid, serving as excitatory central neurotransmitter, exist in different parts of hypothalamus, can stimulate the feeding behavior of animals, activate receptors in the lateral ventricle of the hypothalamus to stimulate the feeding of the animals, a glutamic acid receptor agonist, N-methyl-D-aspartic acid (NMDA), the receptor of Glu involved in appetite stimulation is selectively positioned in LHA, and the LHA can synthesize part of appetite promoting factors such as neuropeptide Y (NPY), GAL and the like; N-methyl-D-aspartate (NMDA) receptors are present in the presynaptic membrane (excitatory nerve endings), modulating the positive feedback of the receptor modulating the release of excitatory amino acids; aspartic acid acts on the long-term reinforcement (memory process) of neurons, and stimulates and eats the Glu transmitter to generate 'favor', so that the eating behavior of the neurons is changed, and the neurons generate dependence. GABA can inhibit the hyperexcitability of the brain, GABA is an inhibitory nerve conduction substance existing in the brain and spinal cord of an animal, is obtained by the catalytic conversion of Glu, needs to be converted and generated in a long-time process, participates in regulating various advanced functions such as food intake, memory, sleep and the like, GABA enables the animal to be quiet by preventing the hyperexcitability of the brain and relieving the stress capacity, promotes the sleep, reduces consumption, regulates the food intake of the animals and promotes the growth; glutamic acid, N methyl-D aspartic acid (NMDA) and aspartic acid belong to acid taste peptides, and amino acid is connected by a peptide chain to form compound small-molecule bioactive polypeptide, and the peptide has a food calling effect and improves the feed intake and the feed utilization efficiency; the yeast extract is obtained by fermenting and enzymolyzing yeast and molasses serving as raw materials by adopting a bioengineering technology, and carrying out enzymolysis on protein, nucleic acid and the like in yeast cells to obtain a product; the invention innovatively researches the effect of enhancing the digestion function, achieves the aims of durably and stably improving the food intake and the conversion utilization rate, and has similar pharmacological action with thiamine hydrochloride for experiments; mainly takes the 'inhibition of the activity of cholinesterase' which is a conductive neurotransmitter as a key point, and extracts essential components from compatible plant extracts; plant compound components such as flavonoids, saponin glycoside, sterol, polysaccharide, polyphenol, alkene, terpenes, etc.; comprises N kinds of plants of fern family, including fern family, Phasianidae Phasiana, Adiantum capillus-veneris, Achillea Wilsonii, Coprinus nitidum, Coprinus comatus, Coprinus malabaricus, Coprinus kuetschaticus, Coprinus caninum, Cyrtomium fortunei, Cyperus rotundus, Cryptomeria fortunei, cortex Phellodendri, radix Paeoniae, cortex et radix Polygalae, rhizoma Acori Graminei, and fructus Aurantii Immaturus; preferably, a variety of formulations are contemplated, including all plant extracts that act in a "cholinesterase activity inhibiting" mechanism of action; the fern natural resources are rich, the purification biological process is adopted for enzymolysis and extraction, and the wall-broken plant cells are adopted, so that the advantages of releasing components in the plant cells, high leaching rate, low cost, no need of special equipment, large-scale industrial production and the like are achieved; pharmacological action; the compound has the effects of inhibiting cholinesterase activity, stimulating salivary secretion, promoting digestion of food, and promoting digestion, eating more quickly and growth quickly; acetylcholine (one of neurotransmitters) affects nerve conduction, and may cause disorders such as slow gastrointestinal motility, decreased digestive juice secretion in the digestive tract, inappetence, dyspepsia, etc.; wherein cortex Phellodendri also has effects of promoting pancreas secretion, and digesting sugar, protein and fat; cortex et radix Polygalae has tranquilizing and hypnotic effects; the plant extract contains various effective compound components, peptides and other traditional Chinese medicine components, and has the functions of activating the secretion of neurotrophic factors, enhancing the memory function, resisting oxidation, eliminating free radicals, regulating blood sugar, regulating the immune function, promoting the growth and the like; the invention has reasonable design and is worth popularizing.

The invention and its embodiments have been described above, without this being limitative. In summary, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the invention as defined by the appended claims.