阅读说明：本技术 一种微乳凝胶面膜 (Microemulsion gel mask ) 是由 董玲 于 2021-11-02 设计创作，主要内容包括：本发明公开了一种微乳凝胶面膜,先以将山茶油、维生素E琥珀酸酯、失水山梨醇单油酸酯、PEG-40氢化蓖麻油形成CO/VES微乳,然后用CO/VES微乳分别溶胀透明质酸、海藻酸钠和卡波姆980,接着将尿囊素、黑枸杞提取物、燕麦提取物、甘油、竹叶黄酮,植物提取物加入到CO/VES微乳中,最后混合,并添加三乙醇胺搅拌均匀即得微乳凝胶面膜；所述的植物提取物由厚朴、透骨草、木芙蓉、肉桂提取得到；黑枸杞提取物由酿酒酵母发酵提取；燕麦提取物由黄伞真菌发酵提取；所制备的微乳凝胶面膜具有补水保湿、经皮吸收效果好、抗衰老抗皱、抗黑色素能力强、稳定性高、抗菌率高、抗菌谱广、无助表面活性剂、不添加化学防腐剂、表面活性剂含量低,安全性好。(The invention discloses a microemulsion gel mask, which is prepared by forming CO/VES microemulsion by camellia oil, vitamin E succinate, sorbitan monooleate and PEG-40 hydrogenated castor oil, then respectively swelling hyaluronic acid, sodium alginate and carbomer 980 by using the CO/VES microemulsion, then adding allantoin, lycium ruthenicum extract, oat extract, glycerol and bamboo leaf flavone into the CO/VES microemulsion, finally mixing, adding triethanolamine and uniformly stirring to obtain the microemulsion gel mask; the plant extract is extracted from cortex Magnolia officinalis, herba Speranskiae Tuberculatae, flos Hibisci Mutabilis, and cortex Cinnamomi; the Lycium ruthenicum Murr extract is prepared by fermenting and extracting Saccharomyces cerevisiae; the oat extract is prepared by fermenting and extracting Pholiota adiposa fungus; the prepared micro-emulsion gel mask has the advantages of moisturizing, good percutaneous absorption effect, anti-aging, anti-wrinkle, strong anti-melanin capability, high stability, high antibacterial rate, wide antibacterial spectrum, no cosurfactant, no chemical preservative, low surfactant content and good safety.)

1. The micro-emulsion gel mask is characterized by being prepared by the following steps:

s1, camellia oil is used as a fat-soluble component solvent, vitamin E succinate is added, the camellia oil is compounded with a lipophilic sorbitan monooleate surfactant and a hydrophilic surfactant PEG-40 hydrogenated castor oil, deionized water is stirred at the temperature of 55-65 ℃, oleic acid, linoleic acid and linolenic acid containing hydroxyl groups in the camellia oil are combined with water molecules through hydrogen bond action to form microemulsion, and the vitamin E succinate is used for expanding the microemulsion region of the camellia oil to obtain the nano-sized CO/VES microemulsion;

s2, adding the CO/VES microemulsion into moisturizing hyaluronic acid, moisturizing sodium alginate and gel carbomer respectively, and swelling to obtain solution A, solution B and solution C;

s3, heating and solubilizing allantoin, lycium ruthenicum Murr extract, oat extract, glycerin, bamboo leaf flavone and plant extracts obtained by extracting magnolia officinalis, garden balsam stem, cotton rose and cinnamon at 38-42 ℃, and synergistically inhibiting bacteria by using the bamboo leaf flavone, the magnolia officinalis, cotton rose leaf, garden balsam stem and cinnamon through different growth cycles of acting thalli to obtain solution D;

s4, adding A, B, C into the solution D, adding triethanolamine, stirring gently with a glass rod, and neutralizing carboxyl on carbomer 980 to form a polymer stable structure, thus obtaining the microemulsion gel mask.

2. The microemulsion-gel mask as claimed in claim 1, wherein the lycium ruthenicum extract is prepared by the following steps: drying Lycium ruthenicum Murr at 60 deg.C, pulverizing, and sieving with 20 mesh sieve to obtain dried powder of Lycium ruthenicum Murr; taking 15g of lycium ruthenicum mill dry powder, adding 300mL of water, sterilizing, adding 2% of saccharomyces cerevisiae, fermenting for 48 hours at 37 ℃, sterilizing the fermentation liquor at high temperature, centrifuging at 10000r/min to obtain supernatant, concentrating, and drying to obtain the lycium ruthenicum mill extract.

3. The microemulsion gel mask as claimed in claim 1, wherein the oat extract is prepared by the following steps: drying oat at 60 ℃, crushing, sieving with a 20-mesh sieve to obtain dry oat powder, taking 15g of the dry oat powder, adding 300mL of water, inoculating 2% of pholiota adiposa fungus after sterilization, fermenting for 48h under the conditions of pH =5 and 28 ℃, sterilizing at high temperature, then adding 1% of alpha-amylase into fermentation liquor, heating to 80 ℃, reacting for 0.5h, removing protein by adopting a sevaeg method, washing for 2 times, then adding 60% of ethanol, precipitating with ethanol twice, and collecting precipitates to obtain an oat extract; in the sevaeg method, the sevage reagent in the reagent consists of chloroform and n-butyl alcohol according to the volume ratio of 4: 1, and the addition amount of the sevage reagent is 50 percent of the total volume of the fermentation liquor.

4. The microemulsion gel mask as claimed in claim 1, wherein the extraction method of the plant extract comprises: cleaning 4g of mangnolia officinalis, 10g of garden balsam stem, 8g of cotton rose leaf and 4g of cinnamon, drying at 50 ℃, then crushing and sieving with a 40-mesh sieve, adding 78% ethanol solution, refluxing at 67 ℃ for 3h, removing filter residue, centrifuging at 15000r/min, collecting supernatant, and concentrating under reduced pressure to obtain the natural extract.

5. The microemulsion gel mask as claimed in claim 1, wherein the mass-volume ratio of camellia oil, vitamin E succinate, sorbitan monooleate, PEG-40 hydrogenated castor oil and deionized water is: 2.3-2.5g, 0.35-0.45g, 0.62-0.84g, 3.51-3.98g and 30-50 mL.

6. The microemulsion gel mask as claimed in claim 1, wherein the ratio of CO/VES microemulsion, hyaluronic acid, sodium alginate, carbomer 980, allantoin, Lycium ruthenicum extract, oat extract, glycerin, bamboo leaf flavone, plant extract and triethanolamine in steps S2-S4 is: 40-60 parts, 2-4 parts, 0.8-1.2 parts, 1.8-2.4 parts, 0.8-1.2 parts, 4-6 parts, 3-5 parts, 4-5 parts, 0.4-0.8 part, 1.5-2.5 parts and 1.2-1.8 parts.

Technical Field

The invention relates to the field of emulsification, in particular to a micro-emulsion gel mask.

Background

The facial mask is a 'fast-moving product' of personal care cosmetics by virtue of the characteristics of changing skin types conveniently and rapidly. The sleep mask is a leave-on type, the use is more convenient, various water-soluble active ingredients are loaded in a commercial product by taking water and alcohol as solvents, but the long-term use of the alcohol can cause skin irritation, meanwhile, the loading of fat-soluble cosmetic active ingredients is limited by the alcohol-water solvent, and the defect is overcome by adding a large amount of surfactants and cosurfactants to prepare a micro emulsion. The use of a large amount of surfactant and co-surfactant can cause safety problems such as irritation of skin mucosa. Therefore, the commonly adopted method for solving the problems of poor compatibility and safety defects is as follows: firstly, the safety of the microemulsion is improved by using a biocompatible surfactant, a cosurfactant and alcohol; secondly, the formula proportion is continuously optimized, the microemulsion area is improved through screening and compounding of the surfactant, the cosurfactant and the medium-low chain alcohol, the use amount of the surfactant, the cosurfactant and the alcohol is reduced as far as possible on the basis of meeting the drug-loading capacity, and the effect is not obvious.

In addition, chemical preservatives such as parabens and methylisothiazolinones which are widely used in facial masks also have certain irritation to skin, and many plant extracts have very excellent bacteriostatic activity and have potential to become cosmetic preservatives. However, at present, plant extracts have poorer preservative efficacy as cosmetic preservatives than chemical preservatives, and are liable to cause product contamination. Therefore, the development of a safe and mild natural preservative system with high preservative efficacy and low price to completely or partially replace chemical preservatives has very important practical significance.

Disclosure of Invention

In view of the above situation, the invention aims to provide a micro-emulsion gel mask, which is safe and mild, has high antiseptic efficacy, good stability and good compatibility of each component, does not use cosurfactant and chemical preservatives, and has the effects of moisturizing, whitening and resisting aging.

The technical scheme for solving the problem is as follows:

a micro-emulsion gel mask is prepared by the following steps:

s1, camellia oil is used as a fat-soluble component solvent, vitamin E succinate is added, the camellia oil is compounded with a lipophilic sorbitan monooleate surfactant and a hydrophilic surfactant PEG-40 hydrogenated castor oil, deionized water is stirred at the temperature of 55-65 ℃, oleic acid, linoleic acid and linolenic acid containing hydroxyl groups in the camellia oil are combined with water molecules through hydrogen bond action to form microemulsion, and the vitamin E succinate is used for expanding the microemulsion region of the camellia oil to obtain the nano-sized CO/VES microemulsion;

s2, adding the CO/VES microemulsion into moisturizing hyaluronic acid, moisturizing sodium alginate and gel carbomer respectively, and swelling to obtain solution A, solution B and solution C;

s3, heating and solubilizing allantoin, lycium ruthenicum Murr extract, oat extract, glycerin, bamboo leaf flavone and plant extracts obtained by extracting magnolia officinalis, garden balsam stem, cotton rose and cinnamon at 38-42 ℃, and synergistically inhibiting bacteria by using the bamboo leaf flavone, the magnolia officinalis, cotton rose leaf, garden balsam stem and cinnamon through different growth cycles of acting thalli to obtain solution D;

s4, adding A, B, C into the solution D, adding triethanolamine, stirring gently with a glass rod, and neutralizing carboxyl on carbomer 980 to form a polymer stable structure, thus obtaining the microemulsion gel mask.

Further, the mass volume ratio of the camellia oil, the vitamin E succinate, the sorbitan monooleate, the PEG-40 hydrogenated castor oil and the deionized water is as follows: 2.3-2.5g, 0.35-0.45g, 0.62-0.84g, 3.51-3.98g and 30-50 mL.

Further, in the steps S2-S4, the ratio of CO/VES microemulsion, hyaluronic acid, sodium alginate, carbomer 980, allantoin, Lycium ruthenicum Murr extract, oat extract, glycerol, bamboo leaf flavone, plant extract and triethanolamine is as follows: 40-60 parts, 2-4 parts, 0.8-1.2 parts, 1.8-2.4 parts, 0.8-1.2 parts, 4-6 parts, 3-5 parts, 4-5 parts, 0.4-0.8 part, 1.5-2.5 parts and 1.2-1.8 parts.

Further, the lycium ruthenicum extract is prepared by the following steps: drying Lycium ruthenicum Murr at 60 deg.C, pulverizing, and sieving with 20 mesh sieve to obtain dried powder of Lycium ruthenicum Murr; taking 15g of lycium ruthenicum mill dry powder, adding 300mL of water, sterilizing, adding 2% of saccharomyces cerevisiae, fermenting for 48 hours at 37 ℃, sterilizing the fermentation liquor at high temperature, centrifuging at 10000r/min to obtain supernatant, concentrating, and drying to obtain the lycium ruthenicum mill extract.

Further, the oat extract is prepared by the following steps: drying oat at 60 ℃, crushing, sieving with a 20-mesh sieve to obtain dry oat powder, taking 15g of the dry oat powder, adding 300mL of water, inoculating 2% of pholiota adiposa fungus after sterilization, fermenting for 48h under the condition that the pH is 5 and the temperature is 28 ℃, sterilizing at high temperature, then adding 1% of alpha-amylase into fermentation liquor, heating to 80 ℃, reacting for 0.5h, removing protein by adopting a sevaeg method, washing for 2 times, then adding 60% of ethanol, precipitating for two times, and collecting precipitate to obtain an oat extract; in the sevaeg method, the sevage reagent in the reagent consists of chloroform and n-butyl alcohol according to the volume ratio of 4: 1, and the addition amount of the sevage reagent is 50 percent of the total volume of the fermentation liquor.

Further, the extraction method of the plant extract comprises the following steps: cleaning 4g of mangnolia officinalis, 10g of garden balsam stem, 8g of cotton rose leaf and 4g of cinnamon, drying at 50 ℃, then crushing and sieving with a 40-mesh sieve, adding 78% ethanol solution, refluxing at 67 ℃ for 3h, removing filter residue, centrifuging at 15000r/min, collecting supernatant, and concentrating under reduced pressure to obtain the natural extract.

Camellia oil is rich in nutrition, and contains fatty acid (no oil)93% of saturated fatty acid, wherein the oleic acid is 82%, the linoleic acid is 11%), camellin,Tea polyphenolsSaponins, tannins, and rich inFlavonoid substanceAndsqualeneAnti-fatigue, anti-low temperature stress, anti-lipid oxidation, anti-radiation, anti-bacterial, anti-allergic skin reaction; the squalene contained in the camellia oil is very easy to be absorbed by skin, enhances the skin resistance, has the functions of moistening and maintaining, mainly maintains the skin moisture, moistens and nourishes the skin, enables the skin to recover the natural elasticity, eliminates and delays the appearance of wrinkles, slows down the skin aging speed, resists ultraviolet rays, has the function of alleviating sun burn, and does not burn the skin.

The main effective component of the bamboo leaf flavonoid is the carbon glycoside flavonoid, and the 4 characteristic carbon glycosides are orientin, isoorientin, vitexin and isovitexin respectively; the bamboo leaf flavone has mild flavor, has the faint scent of bamboo, and has multiple biological effects of resisting oxidation, whitening, eliminating free radicals, resisting radiation, inhibiting bacteria and resisting inflammation.

The lycium ruthenicum extract contains a large amount of lycium ruthenicum polysaccharide and amino acid by carrying out fermented glutinous rice yeast fermentation on lycium ruthenicum, and has the effects of removing free radicals, resisting oxidation, delaying aging and the like.

The oat extract obtained by fermenting the oat with pholiota adiposa fungus contains a large amount of beta-glucan, the cytotoxicity of the oat is greatly reduced after the oat is fermented with pholiota adiposa, the transdermal absorption is good, and the effects of resisting aging and inhibiting melanin are improved.

The natural bacteriostatic extracts mainly use polyphenol, flavone, saponin, alkaloid, organic acid and other substances in plants to achieve bacteriostatic effects, a single plant preservative system has the defects of poor bacteriostatic effect, narrow bacteriostatic spectrum and the like, so different plant extracts with bacteriostatic activity are properly compounded to obtain good bacteriostatic effect and have broad-spectrum bacteriostatic activity, the application discovers that mangnolia officinalis, cotton rose hibiscus leaf, garden burnet, dandelion, magnolia officinalis, violet, garden balsam stem, cinnamon and grassleaved sweetflag are mixed together with bamboo leaf flavone in proportion in repeated experiments in the bacteriostatic plants, the mutual synergy of the effective components can be realized, the synergistic effect is achieved by acting different growth cycles of thalli, and the microemulsion gel mask prepared by the natural bacteriostatic extracts has wider antibacterial range, strong bacteriostatic ability and long effective period.

Due to the adoption of the technical scheme, compared with the prior art, the invention has the following advantages:

1. the camellia oil microemulsion takes camellia oil as a fat-soluble component solvent, vitamin E succinate is added, and the camellia oil, a lipophilic sorbitan monooleate surfactant, a hydrophilic surfactant PEG-40 hydrogenated castor oil and deionized water are compounded to prepare the microemulsion. Dissolving the lycium ruthenicum extract, the oat extract, the plant extract and the bamboo leaf flavone in the obtained microemulsion, mixing the dissolved microemulsion with the microemulsion of hyaluronic acid, sodium alginate and carbomer 980, and adding triethanolamine to prepare a microemulsion gel mask, wherein the microemulsion particles are in a nanometer level, and the microemulsion gel mask is formed by combining hydroxyl groups on unsaturated fatty acids such as lycium ruthenicum polysaccharide, oat beta-glucan and oleic acid, linoleic acid and linolenic acid in camellia oil with water-hydrogen bonds through a small amount of surfactant, so that a cosurfactant is not needed, the microemulsion gel mask is more stable, has weak acidity, high solubility of fat-soluble active ingredients and high percutaneous absorption rate, and has strong antibacterial, anti-aging, anti-oxidation and anti-melanin capabilities;

2. the micro-emulsion gel mask disclosed by the invention has good moisturizing effect, good percutaneous absorption effect, strong antibacterial, anti-aging, anti-wrinkle and anti-melanin capabilities, and sensory and physicochemical indexes meet the QB/T2872-2007 standard; the microemulsion gel mask has high stability, high antibacterial rate, wide antibacterial spectrum and long service time; no cosurfactant is used, no chemical preservative is added, the content of the surfactant is low, no skin irritation is caused, and the safety is good.

Detailed Description

Example 1

A micro-emulsion gel mask is prepared by the following steps:

s1, mixing 2.3g of camellia oil, 0.35g of vitamin E succinate, 0.62g of sorbitan monooleate and 3.51g of PEG-40 hydrogenated castor oil in a vortex manner, heating to 55 ℃, and then dropwise adding 30mL of deionized water under stirring at 600r/min until a clear and transparent microemulsion is formed to obtain a CO/VES microemulsion;

s2, swelling 2 parts of hyaluronic acid, 0.8 part of sodium alginate and 1.8 parts of carbomer 980 by using 40 parts of CO/VES microemulsion respectively, and recording the solutions as solution A, solution B and solution C respectively;

s3, adding 0.8 part of allantoin, 4 parts of lycium ruthenicum extract, 3 parts of oat extract, 4 parts of glycerol, 0.4 part of bamboo leaf flavone and 1.5 parts of plant extract into CO/VES microemulsion, heating and dissolving at 38 ℃, and recording the solution as solution D;

s4, adding A, B, C into the solution D, adding 1.2 parts of triethanolamine, and stirring gently with a glass rod to obtain the microemulsion gel mask.

The lycium ruthenicum extract is prepared by the following steps: drying Lycium ruthenicum Murr at 60 deg.C, pulverizing, and sieving with 20 mesh sieve to obtain dried powder of Lycium ruthenicum Murr; taking 15g of lycium ruthenicum mill dry powder, adding 300mL of water, sterilizing, adding 2% of saccharomyces cerevisiae, fermenting for 48 hours at 37 ℃, sterilizing the fermentation liquor at high temperature, centrifuging at 10000r/min to obtain supernatant, concentrating, and drying to obtain lycium ruthenicum mill extract; the saccharomyces cerevisiae is provided by food brewing research institute.

The oat extract is prepared by the following steps: drying oat at 60 ℃, crushing, sieving with a 20-mesh sieve to obtain dry oat powder, taking 15g of the dry oat powder, adding 300mL of water, inoculating 1% of pholiota adiposa fungus after sterilization, fermenting for 48h under the condition that the pH is 5 and the temperature is 28 ℃, sterilizing at high temperature, then adding 1% of alpha-amylase into fermentation liquor, heating to 80 ℃, reacting for 0.5h, removing protein by adopting a sevaeg method, washing for 2 times, then adding 60% of ethanol, precipitating for two times, and collecting precipitate to obtain an oat extract; in the sevaeg method, a sevage reagent in the reagent consists of chloroform and n-butyl alcohol according to the volume ratio of 4: 1, and the addition amount of the sevage reagent is 50 percent of the total volume of the fermentation liquor; the pholiota adiposa fungus is provided by a key laboratory for researching and developing plant resources in Beijing.

The extraction method of the plant extract comprises the following steps: cleaning 4g of mangnolia officinalis, 10g of garden balsam stem, 8g of cotton rose leaf and 4g of cinnamon, drying at 50 ℃, then crushing and sieving with a 40-mesh sieve, adding 78% ethanol solution, refluxing at 67 ℃ for 3h, removing filter residue, centrifuging at 15000r/min, collecting supernatant, and concentrating under reduced pressure to obtain the natural extract.

Example 2

A micro-emulsion gel mask is prepared by the following steps:

s1, mixing 2.4g of camellia oil, 0.40g of vitamin E succinate, 0.73g of sorbitan monooleate and 3.74g of PEG-40 hydrogenated castor oil in a vortex manner, heating to 60 ℃, and dropwise adding 40mL of deionized water under the stirring of 700r/min until a clear and transparent microemulsion is formed to obtain the CO/VES microemulsion;

s2, swelling 3 parts of hyaluronic acid, 1.0 part of sodium alginate and 2.1 parts of carbomer 980 by using 50 parts of CO/VES microemulsion respectively, and recording the solutions as solution A, solution B and solution C respectively;

s3, adding 1.0 part of allantoin, 5 parts of lycium ruthenicum extract, 4 parts of oat extract, 4.5 parts of glycerol, 0.6 part of bamboo leaf flavone and 2.0 parts of plant extract into CO/VES microemulsion, heating and dissolving at 40 ℃, and recording the solution as solution D;

s4, adding A, B, C into the solution D, adding 1.5 parts of triethanolamine, and stirring gently with a glass rod to obtain the microemulsion gel mask.

The lycium ruthenicum extract is prepared by the following steps: drying Lycium ruthenicum Murr at 60 deg.C, pulverizing, and sieving with 20 mesh sieve to obtain dried powder of Lycium ruthenicum Murr; taking 15g of lycium ruthenicum mill dry powder, adding 300mL of water, sterilizing, adding 2% of saccharomyces cerevisiae, fermenting for 48 hours at 37 ℃, sterilizing the fermentation liquor at high temperature, centrifuging at 10000r/min to obtain supernatant, concentrating, and drying to obtain lycium ruthenicum mill extract; the saccharomyces cerevisiae is provided by food brewing research institute.

The oat extract is prepared by the following steps: drying oat at 60 ℃, crushing, sieving with a 20-mesh sieve to obtain dry oat powder, taking 15g of the dry oat powder, adding 300mL of water, inoculating 1% of pholiota adiposa fungus after sterilization, fermenting for 48h under the condition that the pH is 5 and the temperature is 28 ℃, sterilizing at high temperature, then adding 1% of alpha-amylase into fermentation liquor, heating to 80 ℃, reacting for 0.5h, removing protein by adopting a sevaeg method, washing for 2 times, then adding 60% of ethanol, precipitating for two times, and collecting precipitate to obtain an oat extract; in the sevaeg method, a sevage reagent in the reagent consists of chloroform and n-butyl alcohol according to the volume ratio of 4: 1, and the addition amount of the sevage reagent is 50 percent of the total volume of the fermentation liquor; the pholiota adiposa fungus is provided by a key laboratory for researching and developing plant resources in Beijing.

The extraction method of the plant extract comprises the following steps: cleaning 4g of mangnolia officinalis, 10g of garden balsam stem, 8g of cotton rose leaf and 4g of cinnamon, drying at 50 ℃, then crushing and sieving with a 40-mesh sieve, adding 78% ethanol solution, refluxing at 67 ℃ for 3h, removing filter residue, centrifuging at 15000r/min, collecting supernatant, and concentrating under reduced pressure to obtain the natural extract.

Example 3

A micro-emulsion gel mask is prepared by the following steps:

s1, mixing 2.5g of camellia oil, 0.45g of vitamin E succinate, 0.84g of sorbitan monooleate and 3.98g of PEG-40 hydrogenated castor oil in a vortex manner, heating to 65 ℃, and then dropwise adding 50mL of deionized water under stirring at 800r/min until a clear and transparent microemulsion is formed to obtain a CO/VES microemulsion;

s2, swelling 4 parts of hyaluronic acid, 1.2 parts of sodium alginate and 2.4 parts of carbomer 980 by using 60 parts of CO/VES microemulsion respectively, and recording the solutions as solution A, solution B and solution C respectively;

s3, adding 1.2 parts of allantoin, 6 parts of lycium ruthenicum extract, 5 parts of oat extract, 5 parts of glycerol, 0.8 part of bamboo leaf flavone and 2.5 parts of plant extract into CO/VES microemulsion, heating and dissolving at 42 ℃, and recording the solution as solution D;

s4, adding A, B, C into the solution D, adding 1.8 parts of triethanolamine, and gently and uniformly stirring by using a glass rod to obtain the micro-emulsion gel mask.

The lycium ruthenicum extract is prepared by the following steps: drying Lycium ruthenicum Murr at 60 deg.C, pulverizing, and sieving with 20 mesh sieve to obtain dried powder of Lycium ruthenicum Murr; taking 15g of lycium ruthenicum mill dry powder, adding 300mL of water, sterilizing, adding 2% of saccharomyces cerevisiae, fermenting for 48 hours at 37 ℃, sterilizing the fermentation liquor at high temperature, centrifuging at 10000r/min to obtain supernatant, concentrating, and drying to obtain lycium ruthenicum mill extract; the saccharomyces cerevisiae is provided by food brewing research institute.

The oat extract is prepared by the following steps: drying oat at 60 ℃, crushing, sieving with a 20-mesh sieve to obtain dry oat powder, taking 15g of the dry oat powder, adding 300mL of water, inoculating 1% of pholiota adiposa fungus after sterilization, fermenting for 48h under the condition that the pH is 5 and the temperature is 28 ℃, sterilizing at high temperature, then adding 1% of alpha-amylase into fermentation liquor, heating to 80 ℃, reacting for 0.5h, removing protein by adopting a sevaeg method, washing for 2 times, then adding 60% of ethanol, precipitating for two times, and collecting precipitate to obtain an oat extract; in the sevaeg method, a sevage reagent in the reagent consists of chloroform and n-butyl alcohol according to the volume ratio of 4: 1, and the addition amount of the sevage reagent is 50 percent of the total volume of the fermentation liquor; the pholiota adiposa fungus is provided by a key laboratory for researching and developing plant resources in Beijing.

The extraction method of the plant extract comprises the following steps: cleaning 4g of mangnolia officinalis, 10g of garden balsam stem, 8g of cotton rose leaf and 4g of cinnamon, drying at 50 ℃, then crushing and sieving with a 40-mesh sieve, adding 78% ethanol solution, refluxing at 67 ℃ for 3h, removing filter residue, centrifuging at 15000r/min, collecting supernatant, and concentrating under reduced pressure to obtain the natural extract.

Comparative example 1

In example 2, the ingredients of the lycium ruthenicum extract in the microemulsion gel mask are removed, and the rest ingredients are not changed, so that the microemulsion gel mask is obtained.

Comparative example 2

In example 2, the ingredients of the oat extract in the microemulsion gel mask were removed, and the rest were not changed to obtain the microemulsion gel mask.

Comparative example 3

In the example 2, the ingredients of the bamboo leaf flavone in the microemulsion gel mask are removed, and the rest ingredients are not changed, so that the microemulsion gel mask is obtained.

Test example 1

Characterization of the microemulsion gel masks prepared in example 2 and comparative examples 1-3:

(1) appearance property: observing the appearance of the microemulsion gel mask by a visual inspection method, and inspecting the clarity and the uniformity of the microemulsion gel mask; (2) measurement of pH: measuring the pH value of the microemulsion gel mask by a pH meter at 25 ℃, repeatedly measuring for three times, and recording the result; (3) measurement of particle size: measuring the particle size and the polydispersity index (PDI) of a sample by using a Malvern laser particle sizer under the conditions that the temperature is 25 ℃ and the scattering angle is 90 ℃, diluting the sample by 50 times, and measuring in parallel for three times, wherein the final result is represented by the average value +/-standard deviation; (4) and (3) measuring the viscosity: measuring the viscosity of the sample by using an NDJ-5S type digital viscometer at 25 ℃, wherein the viscosity value is the average value of 3 measurements, and the result is expressed as the average value +/-standard deviation; (5) and (3) investigating the stability of the microemulsion gel mask: carrying out heat resistance and cold resistance investigation on a sample according to QB/T2872-2007 standard, and comparing whether each physical and chemical index of the microemulsion gel mask has obvious change before and after temperature change; the results are shown in Table 1:

from the results in table 1, compared with comparative examples 1-3, the microemulsion gel mask prepared in example 2 is semitransparent gel-like, uniform in appearance, has a pH of 5.6 ± 0.1, a good pH value and a moderate viscosity of 25.96 ± 0.12Pa · s, has fragrance meeting the specified fragrance type, is free from substance precipitation after being placed for several months, and has no change in appearance, and heat and cold resistance experiments show that the microemulsion gel mask is kept at 40 ± 1 ℃ and-5 ± 1 ℃ for 24 hours, and has no explicit changes in appearance, pH and viscosity after being restored to room temperature, while comparative examples 1-3 are relatively unstable, which indicates that the sensory and physicochemical indexes of the prepared microemulsion gel mask meet the standards of QB/T2872-2007.

Test example 2

Skin safety test:

skin safety testing was performed with a skin repeated open smearing test: selecting 30 qualified volunteers as experimental subjects, taking the bent side of the forearm as a tested part, keeping the area of the tested part to be 3cm multiplied by 3cm, keeping the tested part dry and avoiding contacting other external preparations, uniformly coating the microemulsion gel mask prepared in example 2 on the tested part for 2 times every day for 7 days continuously, and observing skin reaction, wherein if 3 or more skin reactions occur in the process, whether the test is continued or not is determined according to specific conditions, the skin reaction repeated open type smearing test skin reaction judgment standard refers to cosmetic safety technical specification (2015 edition), 0 is no reaction, more than 0 is adverse reaction, and the results are shown in table 2:

as shown in table 2, the blank control group was tested, and 30 subjects did not have any adverse reaction; 30 of the subjects with the microemulsion gel mask prepared in example 2 do not have any adverse reaction, and the microemulsion gel mask prepared in the invention is proved to have higher safety to human skin according to the technical specification of cosmetic safety (2015 edition).

Test example 3

And (3) evaluation of moisturizing effect:

30 healthy female volunteers without a history of allergy were selected between 25-40 years of age. The area of 4cm × 4cm inside the left and right forearms was used as the test site, and the test subject cleaned the test site with clear water before the test and stabilized for 30min at a test temperature of (20-25) ° c and a relative humidity of 50% -60%. Respectively applying 30 + -1 mg/cm to the inner sides of the left and right forearms²The microemulsion gel mask prepared in example 2, comparative examples 1 to 3, or a commercially available sleep mask containing hyaluronic acid and sodium alginate components were measured for the percentage of moisture at each site in 0min, 20min, 40min, 60min, and 120min using an intelligent moisture tester, and each test was repeated five times to obtain an average value, and the results are shown in table 3:

as can be seen from Table 3, the microemulsion gel mask and the commercially available sleeping mask prepared in example 2 and comparative examples 1 to 3 were applied

The moisture content of the skin reaches the highest after 20min, and then slightly decreases, and the average moisture content of the skin after 150min is respectively 32.1%, 30.7%, 29.5%, 30.9% and 26.4%, which are higher than the levels of the skin without being applied; the micro-emulsion gel mask is proved to have better moisturizing effect than the commercial sleeping mask, which is probably because the micro-emulsion carrier is applied and the micro-emulsion contains more oil, so that the micro-emulsion gel mask has better moisturizing effect.

Test example 4

And (3) anti-aging and anti-wrinkle test:

150 volunteers were selected and divided into 5 groups, and the microemulsion gel mask of example 1 and comparative examples 1-3 and the commercially available anti-aging and anti-wrinkle sleeping mask were used for the test parts of both cheeks and canthus, 1 time per day, and after cleansing at night; the changes in the average roughness and average depth of wrinkles were measured 1 day before using the mask and 7d, 14d, 21d, 28d after using a MicroSkinII multifunctional skin mirror image analysis system, and the environment was measured: testing the environmental temperature: 22 ± 3 ℃, humidity: 50+ -5%, and real-time dynamic monitoring was performed, and the mean was calculated, the results are shown in Table 4:

as can be seen from table 4, the microemulsion gel mask prepared in comparative examples 1 to 3 has comparable anti-aging and anti-wrinkle effects to those of the commercially available mask, while the microemulsion gel mask prepared in example 2 has the best effects of improving skin roughness and skin wrinkle value.

Test example 5

Evaluation of whitening efficacy:

150 volunteers aged 25-40 years are selected and divided into four groups, namely a group of example 2, a group of comparative example 1, a group of comparative example 2, a group of comparative example 3 and a group of commercially available sleep masks (commercially available sleep masks with whitening effect), and the selected volunteers are used for 1 time each day after face cleaning at night; the change in skin color was measured 1 day before using the mask and 7d, 14d, 21d, 28d after using a Lab colorimetric system spectrophotometer: continuously testing for 3 times, taking the average value of L, simultaneously using a MEXAMETERMX18 skin pigment instrument to measure the content of the skin black and red pigments, continuously testing for 3 times, taking the average value, testing the environmental temperature: 22 ± 3 ℃, humidity: 50+ -5%, and real-time dynamic monitoring was performed, with results shown in Table 5:

as can be seen from Table 5, the subjects had a clear difference from example 2 before the test, and showed a decreasing trend with the product use time, and a clear decrease in melanin content in the skin relative to comparative examples 1-3 and the commercially available sleep mask; the larger the value of L x of the skin color, the lighter the skin color, and the tendency of the value of L x of the skin color after using example 2 was significantly increased, which was significantly greater than the levels of comparative examples 1 to 3 and commercially available sleep masks and before the test, indicating that the microemulsion gel mask prepared in example 2 can effectively reduce skin melanin and whiten the skin.

Test example 6

And (3) testing the antibacterial effect:

test strains: staphylococcus aureus (fungus), escherichia coli (bacterium), candida albicans (bacterium), pseudomonas aeruginosa (fungus), aspergillus niger (fungus); culture medium: TSB solid medium; SDB solid medium; lecithin Tween 80 nutrient agar culture medium, and tiger red culture medium;

inoculation of the strains: activating and subculturing each strain for 3 generations to obtain bacterial suspension, packaging the microemulsion gel facial masks prepared in example 2 and comparative examples 1-3 into 30g, adding bacterial suspension to make the bacteria content of the sample 1 × 10⁶cfu/mL or, 1X 10⁶cfu/g, containing 1X 10 fungi⁴cfu/mL or 1X 10⁴cfu/g, three replicates per sample were made.

Detecting strains: the sampling method comprises the following steps: weighing 10g of the mixture, adding the mixture into a triangular flask filled with glass beads and 90mL of sterilized normal saline, fully oscillating and uniformly mixing, standing for 15min, taking supernatant as a 1:10 detection solution, and sequentially preparing 10 times diluted concentration detection solutions for later use; after the addition of the bacterial solution, the samples were sampled and detected at 0d, 7d, 14d, 21d and 28d, respectively, by the above-mentioned sampling method. Adding 1mL of the prepared 10-fold diluted detection solution into a sterilized flat plate, adding a proper amount of lecithin Tween 80 nutrient agar culture medium or tiger red culture medium cooled to 45 ℃, and shaking anticlockwise to mix uniformly. Three parallel plates were poured for each dilution. After the plate is solidified, the bacteria are placed in an incubator for culturing for 48h at 37 ℃, and then the count is carried out, and the fungi are placed in the incubator for culturing for 72h at 28 ℃.

Evaluation criteria for efficacy of preservative system:

(1) on day 28, the samples contained bacteria or molds>10³cfu/g (mL), the sample can not pass the challenge experiment of microbial attack, which shows that the preservative system of the sample can not effectively inhibit the microbes, and the product is easily polluted by the microbes during production, storage and use; (2) on day 28, the samples contained bacteria at 10²cfu/g(mL)-10³cfu/g (mL), which conditionally passes challenge experiments, i.e. when the protein or other animal and plant material components in the product are not particularly high, the hygienic environment for production meets the requirements, and the packaging is not easy to generate secondary pollution, the preservative system can be used, otherwise, the preservative system cannot be used; (3) on the 28 th day, the bacteria contained in the sample is 10cfu/g (mL) -100 cfu/g (mL), which shows that the preservative system of the sample has stronger inhibiting and killing effect on microorganisms, and the product is not easily polluted by the microorganisms during production, storage and use through challenge tests; (4) from day 7 onwards, the bacteria in the sample<10cfu/g (mL) shows that the preservative system of the sample has extremely strong killing effect on microorganisms, and the product is not easily polluted by the microorganisms during production, storage and use through challenge tests.

The test results are shown in Table 6:

as can be seen from Table 6, compared with comparative example 3, the micro-emulsion gel mask prepared in example 2 and comparative examples 1-2 has better antiseptic effect on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans, and from 7d, both bacteria and fungi in the sample are less than 10cfu/g (mL), which indicates that the micro-emulsion gel mask prepared in example 2 has extremely strong effect of inhibiting and killing microorganisms and is not easily polluted by microorganisms during production, storage and use.

While the invention has been described in further detail with reference to specific embodiments thereof, it is not intended that the invention be limited to the specific embodiments thereof; for those skilled in the art to which the present invention pertains and related technologies, the extension, operation method and data replacement should fall within the protection scope of the present invention based on the technical solution of the present invention.