阅读说明：本技术 一种芦苇资源化利用工艺 (Reed resource utilization process ) 是由 潘正国 胡明明 李本栋 徐淳 朱霖毅 于 2021-11-09 设计创作，主要内容包括：本发明涉及环保领域,尤其涉及一种芦苇资源化利用工艺,包括以下步骤：步骤1：将芦苇剪成1-5cm段；步骤2：将上述剪成小段的芦苇进行粉碎,粉碎至过1mm筛；步骤3：调节步骤2中的得到物料的含固率至40％-50％,继续粉碎打浆过80目筛；并通过柠檬酸钠控制浆料pH在4-5；步骤4；添加含有淀粉酶、纤维素酶、β-葡萄糖苷酶和半纤维素酶的混合酶,添加量为10FPU/g-40FPU/g；并保持在35-55℃、150-300rpm的条件下酶解72h；步骤5：冷却结晶并过滤,得到葡萄糖、木糖和纤维二塘。该工艺可提升对于芦苇的降解能力,提升得糖效率。(The invention relates to the field of environmental protection, in particular to a reed resource utilization process, which comprises the following steps: step 1: cutting the reed into 1-5cm sections; step 2: crushing the reed cut into small sections, and sieving the crushed reed by a sieve of 1 mm; and step 3: adjusting the solid content of the obtained material in the step 2 to 40-50%, continuously crushing, pulping and sieving by a 80-mesh sieve; controlling the pH value of the slurry to be 4-5 by sodium citrate; step 4; adding a mixed enzyme containing amylase, cellulase, beta-glucosidase and hemicellulase, wherein the addition amount is 10FPU/g-40 FPU/g; and keeping the temperature at 35-55 ℃ and the condition of 150-; and 5: cooling, crystallizing and filtering to obtain glucose, xylose and fiber. The process can improve the degradation capability of the reed and improve the sugar obtaining efficiency.)

1. A reed resource utilization process is characterized in that: the method comprises the following steps:

step 1: cutting the reed into 1-5cm sections;

step 2: crushing the reed cut into small sections, and sieving the crushed reed by a sieve of 1 mm;

and step 3: adjusting the solid content of the obtained material in the step 2 to 40-50%, continuously crushing, pulping and sieving by a 80-mesh sieve; controlling the pH value of the slurry to be 4-5 by sodium citrate;

step 4; adding a mixed enzyme containing amylase, cellulase, beta-glucosidase and hemicellulase, wherein the addition amount is 10FPU/g-40 FPU/g; and keeping the temperature at 35-55 ℃ and the condition of 150-;

and 5: cooling and filtering to obtain a second pond of glucose, xylose and fiber.

2. The reed resource utilization process as claimed in claim 1, wherein: the ratio of the cellulase, the beta-glucosidase and the hemicellulase in the mixed enzyme is 40: 30: 15.

3. The reed resource utilization process as claimed in claim 2, wherein: the addition amount of the mixed enzyme is 20FPU/g-30 FPU/g.

4. The reed resource utilization process as claimed in claim 1, wherein: the reed in the step 1 adopts green reed with water content of 45-65% and/or dry reed with water content of 10-15%.

Technical Field

The invention relates to the field of environmental protection, in particular to a reed resource utilization process.

Background

Cellulose is a biodegradable high molecular polymer synthesized by plants through photosynthesis. The annual yield of the cellulose synthesized by photosynthesis in the nature can reach hundreds of billions of tons, and the cellulose mainly comes from plants such as wood, cotton, grass and the like. The reed is used as a lignocellulose material with high fiber content, belongs to perennial plants, has concentrated growth, easy felling and high yield, and can replace the traditional wood to be used in the fields of packaging, construction and the like. Reed resources in China are rich and widely distributed, and the ecological value and the economic value of reed are more and more valued by scientific and technological workers.

Both natural cellulose and hemicellulose in the wood fiber raw material are polysaccharides, and saccharides such as glucose, xylose and the like can be generated after hydrolysis. However, considering that the lignocellulose content of the reed fiber is higher than that of common straw substances, the prior resource utilization process in the reed has the problems of difficult degradation and unobvious pretreatment effect.

Furthermore, dry reeds are adopted in the current resource utilization process of reeds, and the dry reeds need to be harvested and placed in a warm and dry open environment with good ventilation for natural air drying. But the whole process needs a long time to occupy a larger field, and the cost is higher. At present, no technology for directly processing the harvested green reeds exists.

And finally, the nutrient components of the reed are soluble sugar, starch, cellulose, crude protein, soluble protein and ash, but the reed in different habitats has different proportions of various nutrient components, the content of the reed in the transition zone is only 50-60% in terms of the total of the soluble sugar, the starch and the cellulose, and the content of the soluble starch, the starch and the cellulose in the marsh reed, the dune reed and the salinized reed is more than 60-70%. Aiming at the structure of reed cellulose, the sugar yield by single enzyme degradation is lower.

Based on the defects of the prior art, the scheme aims to provide the reed resource utilization process, the degradation capability of the reed can be improved, and the treatment effect is obviously improved.

Disclosure of Invention

In order to solve the problems, the invention aims to provide a reed resource utilization process, which can improve the degradation capability of the reeds and improve the sugar obtaining efficiency.

In order to achieve the purpose, the invention adopts the following technical scheme:

a reed resource utilization process is characterized in that: the method comprises the following steps:

step 1: cutting the reed into 1-5cm sections;

step 2: crushing the reed cut into small sections, and sieving the crushed reed by a sieve of 1 mm;

and step 3: adjusting the solid content of the obtained material in the step 2 to be 40-50%, continuously crushing, pulping and sieving by a 80-mesh sieve; controlling the pH value of the slurry to be 4-5 by sodium citrate;

step 4; adding a mixed enzyme containing amylase, cellulase, beta-glucosidase and hemicellulase, wherein the addition amount is 10FPU/g-40 FPU/g; and keeping the temperature at 35-55 ℃ and the condition of 150-;

and 5: cooling, crystallizing and filtering to obtain glucose, xylose and fiber.

The invention adopts the technical scheme, which relates to a reed resource utilization process, wherein in the process steps, the reed is cut into small sections and then crushed, and then is continuously crushed and pulped to pass through a 80-mesh sieve, so that reed particles smaller than 80 meshes are obtained, and the solid content of the material is kept between 40 and 50 percent; in this case, a mixed enzyme is added for the enzymatic hydrolysis. The technical effect of the scheme is that on one hand, the solid content of the material is kept between 40% and 50%, and the stirring at 300rpm is performed in the enzymolysis process, so that the mixing degree of the mixed enzyme and the reed particles is increased, and the reed particles can be fully contacted with the mixed enzyme; on the other hand, the reed is cut into sections and then is pulped to obtain reed particles smaller than 80 meshes, so that the contact area between the reed particles and the mixed enzyme can be increased, and the enzymolysis efficiency is improved. Based on the process steps, the degradation capability of the reed can be improved, and the sugar obtaining efficiency is improved.

Preferably, the ratio of the cellulase, the beta-glucosidase and the hemicellulase in the mixed enzyme is 40: 30: 15. The test comparison shows that the neutralization sugar yield of the proportion is the highest.

Preferably, the amount of the mixed enzyme is 20FPU/g to 30 FPU/g. Tests show that when the addition amount of the mixed enzyme is less than 20FPU/g, the sugar yield can be improved by further increasing the content of the mixed enzyme, and when the addition amount of the mixed enzyme is more than 30FPU/g, the effect of further increasing the content of the mixed enzyme on improving the sugar yield is not obvious. Therefore, the preferable range in the scheme is to ensure that the addition amount of the mixed enzyme is 20FPU/g-30FPU/g, and can ensure that a higher sugar yield is obtained on the basis of a proper amount of the mixed enzyme, thereby controlling the cost.

Preferably, the reed in the step 1 is green reed with water content of 45-65% and/or dry reed with water content of 10-15%. In the scheme, not only can dry reed which is naturally dried be adopted, but also green reed which is obtained by harvesting can be adopted, so that the green reed can be directly processed, the steps of storing and air-drying the reed are omitted, and the storage cost is saved.

Detailed Description

The following detailed description of embodiments of the invention is intended to be illustrative, and is not to be construed as limiting the invention.

Experiment 1, compound enzyme proportioning optimization;

the experiment is developed based on the following process steps, parameters in the process steps are adjusted, and the comparison is carried out by setting 4 groups of experiment groups, wherein the experiment groups specifically comprise the following steps:

(I), the test group provides a reed resource utilization process, which comprises the following steps:

step 1: cutting equal amount of rhizoma Phragmitis (green rhizoma Phragmitis with water content of 45-65% and/or dry rhizoma Phragmitis with water content of 10-15%) into 1-5cm sections;

step 2: crushing the reed cut into small sections, and sieving the crushed reed by a sieve of 1 mm;

and step 3: adjusting the solid content of the obtained material in the step 2 to be 40-60%, continuously crushing, pulping and sieving by a 80-mesh sieve; controlling the pH value of the slurry to be 4-5 by sodium citrate;

step 4; respectively adding mixed enzyme (the mixture ratio of the mixed enzyme is shown in table 1) into 4 groups of test groups, wherein the addition amount of the mixed enzyme is 20FPU/g, and the mixed enzyme in each group of test groups contains amylase, cellulase, beta-glucosidase and hemicellulase in different proportions; and keeping the temperature at 35-55 ℃ and the condition of 150-;

and 5: cooling, crystallizing and filtering to obtain glucose, xylose and fiber.

TABLE 1 blending ratio of enzyme

	Amylase	Cellulase enzymes	Beta-glucosidase	Hemicellulase(s)
					Test group 1	30％	30％	20％	20％
Test group 2	40％	30％	15％	15％
					Test group 3	40％	20％	20％	20％
Test group 4	20％	40％	20％	20％

Sugar yield of the test group. The data are shown in table 2:

TABLE 2 yield of mixed enzyme hydrolyzed reed sugar in different proportions

	Glucose	Xylose	Cellobiose
				Test group 1	49.33％	68.43％	27.53％
Test group 2	59.33％	70.32％	27.32％
				Test group 3	56.21％	62.18％	21.51％
Test group 4	39.56％	69.75％	20.43％

According to the experimental comparison, the comprehensive sugar yield of the test group 2 is the highest, and the compound enzyme is selected according to the following mixture ratio: 40% of amylase, 30% of cellulase, 15% of beta-glucosidase and 15% of hemicellulase.

Experiment 2: an experiment for sugar preparation by enzymatic hydrolysis of reed pulp with different enzyme solubilities and different water contents:

(II), the test group provides a reed resource utilization process, which adopts the following steps:

step 1: cutting equal amount of reed into 1-5cm sections;

step 2: crushing the reed cut into small sections, and sieving the crushed reed by a sieve of 1 mm;

and step 3: adjusting the solid content of the obtained material in the step 2, continuously crushing, pulping and sieving by a 80-mesh sieve; controlling the pH value of the slurry to be 4-5 by sodium citrate;

step 4; respectively adding mixed enzyme (40% of amylase, 15% of cellulase and 15% of beta-glucosidase) into the 4 groups of test groups, adjusting the adding amount of the mixed enzyme, and carrying out enzymolysis for 72h under the conditions of 35-55 ℃ and 150-300 rpm;

and 5: cooling, crystallizing and filtering to obtain glucose, xylose and fiber.

Experiments are developed based on the process steps, parameters in the process steps are adjusted, 5 experiment large groups are set, and only the solid content of the slurry in the step 3 is adjusted among the experiment large groups, wherein the solid content is respectively 30%, 40%, 50%, 60% and 70%; and 4 test groups are arranged in each test group, wherein the test groups 1-4 in each test group ensure that the water content of the pulp in the step 3 is consistent, and the difference is only that the addition amount of the mixed enzyme in the step 4 is adjusted to be 10FPU, 20FPU, 30FPU and 40FPU respectively.

In addition, a group of control groups is arranged in each of the 5 experimental large groups, and the control groups adopt the following process steps:

step 1: cutting the same amount of green reed with water content of 45-65% and/or dry reed with water content of 10-15%) into 1-5cm sections;

step 2: crushing the reed cut into small sections, and sieving the crushed reed by a sieve of 1 mm;

and step 3: adjusting the solid content of the materials to the concentration required by a large experimental group, and controlling the pH value of the slurry to be 4-5 by sodium citrate;

step 4; respectively adding mixed enzyme (40% of amylase, 15% of cellulase and 15% of beta-glucosidase) into the 4 groups of test groups, wherein the addition amount is 10FPU/g, and carrying out enzymolysis for 72h under the conditions of 35-55 ℃ and 150-300 rpm;

and 5: cooling, crystallizing and filtering to obtain glucose, xylose and fiber.

Monosaccharide concentration and monosaccharide yield data obtained by an enzymolysis process under different enzyme concentrations and water contents of reed pulp are as follows:

the sugar yield obtained by the above table conversion is as follows

Experiment 2 was analyzed as follows:

1. comparing the test group 1 and the control group in each experimental group in the two tables, the results show that under the same water content and enzyme concentration of the pulp, the yield and concentration of the prepared sugar are higher in the test group 1 after crushing and beating through a 80-mesh sieve than in the control group (non-beating group). Therefore, the contact area of the enzyme and the mixed enzyme can be increased and the enzymolysis efficiency can be improved through the pulping process and the 80-mesh sieve.

2. Compared in each experiment group, under the condition of certain water content of the pulp, the concentration and yield of the sugar after enzyme hydrolysis with the concentration of 20FPU are greatly improved compared with 10 FPU; at a concentration of 30FPU, the yield and concentration of hydrolyzed sugar were increased slowly compared to 20FPU, and at a concentration of 40FPU, the yield and concentration of hydrolyzed sugar were not changed much compared to 30 FPU. Therefore, the preferable range in the scheme is to ensure that the addition amount of the mixed enzyme is 20FPU/g-30FPU/g, and can ensure that a higher sugar yield is obtained on the basis of a proper amount of the mixed enzyme, thereby controlling the cost.

And 3, performing transverse comparison in each experiment group, wherein the concentration and the yield of the sugar are higher than those of other water contents when the solid content is 40-50% under the condition of enzyme addition amount of each concentration.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made in the above embodiments by those of ordinary skill in the art without departing from the principle and spirit of the present invention.