阅读说明：本技术 一种沉香烟用爆珠及其制备方法 (Agilawood blasting bead for cigarette and preparation method thereof ) 是由 杨锦玲 于 2021-01-13 设计创作，主要内容包括：本发明提供了一种沉香烟用爆珠及其制备方法,通过将作为名贵香料和名贵药材的沉香加入烟用爆珠中,并将其利用到香烟中,该香烟抽吸时具有沉香特殊的香味,可以改善烟草的味道,使得烟气入口柔和、留香好,给消费者带来轻松感、舒适感和满足感；通过将沉香净油与果胶、环糊精混合制备成乳化液,提高了沉香烟用爆珠香气释放的持久性；通过本发明的干燥方式,使爆珠的外壳厚度均一,脆性更佳,从而提高捏破手感；通过本发明提取方法提取出来的沉香净油中倍半萜和色酮类成分相对含量稳定,沉香净油稳定性好,倍半萜类成分常温下即可散发香味,色酮在高温时可以裂解成简单芳香性成分,用该沉香净油制备的沉香烟用爆珠还可预防肺癌,具有保健作用。(The invention provides an agilawood bead for cigarette and a preparation method thereof, wherein agilawood serving as rare spice and rare medicinal material is added into the bead for cigarette and is utilized in the cigarette, the cigarette has the special fragrance of agilawood when being smoked, the taste of the tobacco can be improved, the smoke is soft and has good fragrance, and the agilawood bead brings relaxation, comfort and satisfaction to consumers; the agilawood absolute oil, the pectin and the cyclodextrin are mixed to prepare the emulsion, so that the durability of the fragrance release of the exploded beads for the agilawood cigarette is improved; through the drying mode, the shell of the popping beads is uniform in thickness and better in brittleness, so that the hand feeling of pinching and breaking is improved; the agarwood absolute oil extracted by the extraction method has stable relative content of sesquiterpene and chromone components, good stability, fragrance of the sesquiterpene components at normal temperature, and simple aromatic components of chromone which can be cracked at high temperature.)

1. The agilawood blasting bead for the cigarette comprises emulsion and forming liquid, and is characterized in that the emulsion comprises the following raw materials in parts by weight: 15-30 parts of agilawood absolute oil, 1-5 parts of pectin and 1-2 parts of cyclodextrin;

the molding liquid comprises the following raw materials in parts by weight: 1-5 parts of calcium chloride, 1-5 parts of sodium alginate, 5-10 parts of Arabic gum and 5-10 parts of water;

the dosage of the emulsion is 80-100% of the weight of the molding liquid.

2. The popping bead for the agilawood cigarette as claimed in claim 1, wherein the relative content of the sesquiterpene compound in the agilawood absolute is more than or equal to 25%, and the relative content of the chromone compound is more than or equal to 45%.

3. The blasting bead for the agilawood cigarette as claimed in claim 1, wherein the diameter specification of the blasting bead for the agilawood cigarette is 3.0-3.2mm, the content loading is 12.0 +/-1.0 mg/grain, and the crushing pressure is 0.7-1.7 kg.

4. The popping bead for the agilawood cigarette as claimed in claim 1, wherein the extraction method of the agilawood absolute oil comprises the following steps:

s1, pretreatment: pulverizing lignum Aquilariae Resinatum to 10-40 mesh;

s2, extracting the crushed agilawood by a steam distillation method to obtain a fraction 1 and agilawood residues;

s3, heating and refluxing the agilawood residues extracted in the step S2 by using ethanol or ultrasonically extracting at normal temperature, filtering, repeatedly extracting for 3-5 times, combining filtrates, and concentrating the filtrate under reduced pressure until the relative density is 1.0-1.5 to obtain a concentrated solution;

s4, extracting the concentrated solution obtained in the step S3 by using n-butane as a solvent, wherein the volume ratio of the n-butane to the concentrated solution is 1-2:1, the extraction times are 3-5 times, combining the extraction solutions, and then concentrating the extraction solutions under reduced pressure until no solvent exists;

s5, uniformly mixing the fraction 1 and the fraction 2 to obtain the agilawood absolute oil.

5. The popping bead for the agilawood cigarette as claimed in claim 4, wherein in the step S2, the steam distillation method is heating to 90-110 ℃ and distilling for 3-5 h.

6. The popping bead for the agilawood cigarette as claimed in claim 4, wherein in the step S3, the heating reflux is heating to 70-80 ℃ for reflux extraction for 1-2 h.

7. The popping bead for the agilawood cigarette according to claim 4, wherein in the step S3, the ultrasonic frequency of the normal-temperature ultrasonic extraction is 60-100KHz, and the ultrasonic time is 1.5-3 h.

8. The popping bead for the agilawood cigarette as claimed in claim 4, wherein in the step S4, the extraction is carried out at 20-30 ℃.

9. The preparation method of the blasting bead for the agilawood cigarette according to any one of claims 1 to 8, characterized by comprising the following steps:

(1) preparing an emulsion: mixing lignum Aquilariae Resinatum absolute oil with pectin and cyclodextrin under stirring to obtain emulsion;

(2) preparing a forming liquid: stirring water, sodium alginate, calcium chloride and Arabic gum together until the mixture is uniformly mixed to obtain a forming solution;

(3) and (3) shaping the dropping pills: and uniformly dripping the emulsion into the forming liquid, curing and forming, cleaning and drying to obtain the agilawood blasting bead for the cigarette.

10. The preparation method of the popping beads for the agilawood cigarette as claimed in claim 9, wherein in the step (3), the drying is performed at a speed of 5-8r/min, the drying temperature is 20-40 ℃, the drying humidity is 20-40% RH, and the drying time is 1-3 h.

Technical Field

The invention relates to the field of bead blasting for cigarettes, and in particular relates to a bead blasting for agilawood cigarettes and a preparation method thereof.

Background

In the cigarette production technology, in order to reduce the tar content of cigarette products, the tobacco industry in China can reduce the tar content of the cigarette products as much as possible, but the cigarette products with lower tar content can generate less pleasant aroma substances during combustion, so that the mouthfeel of the cigarettes is seriously reduced, and the aroma generated by tobacco leaves of different grades is also different. Therefore, in the prior art, while the tar content in the cigarette is reduced, the flavor is added to the cigarette to maintain the characteristic aroma of the cigarette. Wherein, the method of adding spice into the cigarette is to embed the popping beads in the cigarette filter stick.

The puffing bead is a small rubber bead embedded in the tobacco filter tip, different types of liquid are wrapped in the rubber bead, and a smoker can puff the puffing bead in the smoking process to enable the puffing bead to flow out, so that the cigarette is richer in taste and more fragrant and moist, and the smoker can obtain more comfortable experience. However, the development of the technology of perfuming cigarettes by using the popping beads is not mature, and the prepared popping beads for cigarettes have the defects of poor stability of perfuming ingredients, difficult preservation, lasting fragrance and the like, so that the research on the perfuming raw materials suitable for the popping beads for cigarettes is the most important work at present.

The agilawood is a world rare spice and rare medicinal materials, is pungent and bitter in taste and slightly warm in nature, has the effects of promoting qi circulation, relieving pain, warming middle energizer, relieving vomit, absorbing qi, relieving asthma and the like, and is proved by Japanese clinical trial researches to be a specific medicine for the stomach cancer and a good analgesic medicine. Meanwhile, the agilawood has the effects of tranquilizing and allaying excitement, reducing blood pressure, reducing blood sugar, resisting bacteria, resisting cancer, resisting arrhythmia, resisting myocardial ischemia, inhibiting the central nervous system and the like, and is used as a famous and precious spice, and has light and elegant fragrance, clear gradation and lasting fragrance.

Disclosure of Invention

Aiming at the problems, the invention aims to provide an agilawood blasting bead for cigarettes and a preparation method thereof.

An explosion bead for agilawood cigarette comprises emulsion and forming liquid; the emulsion comprises the following raw materials in parts by weight: 15-30 parts of agilawood absolute oil, 1-5 parts of pectin and 1-2 parts of cyclodextrin;

the molding liquid comprises the following raw materials in parts by weight: 1-5 parts of calcium chloride, 1-5 parts of sodium alginate, 5-10 parts of Arabic gum and 5-10 parts of water;

the dosage of the emulsion is 80-100% of the weight of the molding liquid;

furthermore, the relative content of the sesquiterpene compound in the agilawood absolute is more than or equal to 25%, the relative content of the chromone compound is more than or equal to 45%, and the agilawood absolute with the sesquiterpene compound and the chromone compound in the relative content range has good stability.

Furthermore, the diameter specification of the explosion bead for the agilawood cigarette is 3.0-3.2mm, the content is 12.0 +/-1.0 mg/particle, and the crushing pressure is 0.7-1.7 kg.

Further, the extraction method of the agilawood absolute oil comprises the following steps:

s1, pretreatment: pulverizing lignum Aquilariae Resinatum to 10-40 mesh;

s2, extracting the crushed agilawood by a steam distillation method to obtain a fraction 1 and agilawood residues, wherein the fraction 1 comprises sesquiterpene components easy to volatilize;

s3, heating and refluxing the agilawood residues extracted in the step S2 by using ethanol or ultrasonically extracting at normal temperature, filtering while hot, repeatedly extracting for 3-5 times, combining filtrates, and concentrating the filtrate under reduced pressure until the relative density is 1.0-1.5 to obtain a concentrated solution; wherein the ethanol is 75-95% ethanol solution by volume concentration;

s4, extracting the concentrated solution obtained in the step S3 by using n-butane as a solvent, wherein the volume ratio of the n-butane to the concentrated solution is 1-2:1, the extraction times are 3-5 times, merging the extraction solutions, and then concentrating the extraction solutions under reduced pressure until no solvent exists to obtain a fraction 2, wherein the fraction 2 comprises chromone components and high-boiling point sesquiterpene components;

s5, uniformly mixing the fraction 1 and the fraction 2 to obtain the agilawood absolute oil.

The sesquiterpene component extracted by steam distillation can emit fragrance at normal temperature, sesquiterpene and chromone components extracted from the extracted agilawood residue with ethanol are extracted, and the chromone component and high-boiling point sesquiterpene component cracked into simple aromatic components at high temperature are extracted by using n-butane as a solvent.

Further, in step S2, the steam distillation method is heating to 90-110 ℃ and distilling for 3-5h, wherein the ratio g/ml of the material to the liquid is 1 (2-3).

Further, the heating reflux is reflux extraction for 1-2h by heating to 70-80 ℃.

Further, the ultrasonic frequency of the normal temperature ultrasonic extraction is 60-100KHz, and the ultrasonic time is 1.5-3 h.

Further, in step S4, the extraction is performed at 20-30 ℃, and the extraction is performed at this temperature, so as to avoid component loss caused by cracking of chromone component.

The preparation method of the agilawood blasting bead for the cigarette comprises the following steps:

(1) preparing an emulsion: mixing lignum Aquilariae Resinatum absolute oil with pectin and cyclodextrin under stirring to obtain emulsion;

(2) preparing a forming liquid: stirring water, sodium alginate, calcium chloride and Arabic gum together until the mixture is uniformly mixed to obtain a forming solution;

(3) and (3) shaping the dropping pills: and uniformly dripping the emulsion into the forming liquid, curing and forming, cleaning and drying to obtain the agilawood blasting bead for the cigarette.

Further, in the step (3), the drying is carried out at a speed of 5-8r/min, the drying temperature is 20-40 ℃, the drying humidity is 20-40% RH, the drying time is 1-3h, and the drying is carried out at a speed of 5-8r/min under the conditions of the temperature and the humidity, so that the shell of the dried blasting bead has uniform thickness and better brittleness.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides an agilawood bead for cigarettes and a preparation method thereof, wherein agilawood serving as rare spice and rare medicinal material is added into the bead for cigarettes and is utilized in the cigarettes, and the cigarettes have special fragrance of the agilawood when being smoked, so that the taste of the cigarettes can be improved, the smoke is soft and has good fragrance, and the agilawood bead for cigarettes brings relaxation, comfort and satisfaction to consumers; the agilawood absolute oil, the pectin and the cyclodextrin are mixed to prepare the emulsion, so that the durability of the fragrance release of the exploded beads for the agilawood cigarette is improved.

Furthermore, through the drying mode of the invention, the shell of the popping beads has uniform thickness and better brittleness, thereby improving the hand feeling of pinching and breaking; the relative content of sesquiterpenes and chromone components in the agilawood absolute oil extracted by the extraction method is stable, so that the stability of the agilawood absolute oil is ensured; the sesquiterpene component can emit fragrance at normal temperature, the chromone component can be cracked into simple aromatic components at high temperature, the agilawood neat oil is used for preparing the agilawood blasting bead for the agilawood cigarette, and then the blasting bead for the agilawood cigarette is utilized in the cigarette, so that the agilawood cigarette has elegant fragrance and rich fragrance when the cigarette is smoked, the taste of the tobacco can be improved, the smoke gas is soft in entrance, the fragrance retaining effect is good, and the relaxing feeling, the comfortable feeling and the satisfaction feeling are brought to consumers.

Detailed Description

The invention provides an agilawood cigarette blasting bead and a preparation method thereof, and a person skilled in the art can realize the blasting bead by properly improving process parameters by referring to the content. It is expressly intended that all such alterations and modifications which are obvious to those skilled in the art are deemed to be incorporated herein by reference. While the compositions and methods of preparation and use of the invention have been described in terms of preferred embodiments, it will be apparent to those skilled in the art that variations and modifications, or appropriate alterations and combinations, of the compositions and methods of preparation and use of the invention disclosed herein may be made and practiced without departing from the spirit, scope, and spirit of the invention.

In the specific comparison test of the invention, the test environments of other materials and the like are consistent except for the due differences of each group.

In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.

Example 1

The agilawood blasting bead for the cigarette comprises emulsion and forming liquid, wherein the dosage of the emulsion is 95% of the weight of the forming liquid;

the emulsion comprises the following raw materials in parts by weight: 26g of agilawood absolute oil, 3g of pectin and 2g of cyclodextrin;

the molding liquid comprises the following raw materials in parts by weight: 4g of calcium chloride, 3g of sodium alginate, 6g of Arabic gum and 8 parts of water;

the preparation method of the agilawood absolute oil comprises the following steps: crushing 10kg of agilawood to 20 meshes, heating to 100 ℃ by adopting a steam distillation method, distilling for 3h, and extracting to obtain 20g of fraction 1 and agilawood residues, wherein the g/ml of distilled material liquid is 1: 3; adding lignum Aquilariae Resinatum residue into 75% ethanol solution, heating to 70 deg.C, reflux extracting for 2 hr, filtering while hot, repeating extracting for 3 times, mixing filtrates, and concentrating the filtrate under reduced pressure to relative density of 1.2 to obtain 6L concentrate; extracting the concentrated solution for 5 times at 30 ℃ by using 6L of n-butane as a solvent, combining the extraction solutions, and concentrating the extraction solutions under reduced pressure until no solvent exists to obtain 220 g of fraction 2; the fraction 1 and the fraction 2 are mixed uniformly to obtain 240 g of agilawood absolute oil.

The preparation method of the agilawood blasting bead for the cigarette comprises the following steps:

(1) preparing an emulsion: mixing lignum Aquilariae Resinatum absolute oil with pectin and cyclodextrin under stirring to obtain emulsion;

(2) preparing a forming liquid: stirring water, sodium alginate, calcium chloride and Arabic gum together until the mixture is uniformly mixed to obtain a forming solution;

(3) and (3) shaping the dropping pills: and uniformly dripping the emulsion into the forming liquid, curing and forming, cleaning, then putting into a roller, and drying in a rotating manner at the speed of 6r/min at the drying temperature of 20 ℃ and the drying humidity of 40% RH for 1h to obtain the agilawood blasting beads for cigarettes.

Example 2

The agilawood blasting bead for the cigarette comprises emulsion and forming liquid, wherein the dosage of the emulsion is 80% of the weight of the forming liquid;

the emulsion comprises the following raw materials in parts by weight: 15g of agilawood absolute oil, 1g of pectin and 1g of cyclodextrin;

the molding liquid comprises the following raw materials in parts by weight: 5g of calcium chloride, 5g of sodium alginate, 10g of Arabic gum and 10 parts of water;

the preparation method of the agilawood absolute oil comprises the following steps: crushing 5kg of agilawood raw material to 10 meshes, heating to 90 ℃ by adopting a steam distillation method, distilling for 5 hours, and extracting to obtain 5g of fraction 1 and agilawood residues, wherein the g/ml of distilled material liquid is 1: 2; adding the agilawood residue into an ethanol solution with the volume concentration of 95%, heating to 80 ℃, performing reflux extraction for 1h, filtering while the solution is hot, repeatedly extracting for 3 times, combining filtrates, and concentrating the filtrate under reduced pressure until the relative density is 1.0 to obtain 3L of concentrated solution; extracting the concentrated solution with 5L of n-butane as solvent at 20 deg.C for 3 times, mixing extractive solutions, and concentrating under reduced pressure until there is no solvent to obtain 61g fraction 2; mixing fraction 1 and fraction 2 to obtain 66g of lignum Aquilariae Resinatum absolute oil.

The preparation method of the agilawood blasting bead for the cigarette comprises the following steps:

(1) preparing an emulsion: mixing lignum Aquilariae Resinatum absolute oil with pectin and cyclodextrin under stirring to obtain emulsion;

(2) preparing a forming liquid: stirring water, sodium alginate, calcium chloride and Arabic gum together until the mixture is uniformly mixed to obtain a forming solution;

(3) and (3) shaping the dropping pills: and uniformly dripping the emulsion into the forming liquid, curing and forming, cleaning, then putting into a roller, and drying in a rotating manner at the speed of 8r/min at the drying temperature of 20 ℃ and the drying humidity of 30% RH for 3h to obtain the agilawood blasting bead for cigarettes.

Example 3

The agilawood blasting bead for the cigarette comprises emulsion and forming liquid, wherein the dosage of the emulsion is 90% of the weight of the forming liquid;

the emulsion comprises the following raw materials in parts by weight: 26g of agilawood absolute oil, 3g of pectin and 2g of cyclodextrin;

the molding liquid comprises the following raw materials in parts by weight: 4g of calcium chloride, 3g of sodium alginate, 6g of Arabic gum and 8 parts of water;

the preparation method of the agilawood absolute oil comprises the following steps: crushing 5kg of agilawood raw material to 40 meshes, heating to 110 ℃ by adopting a steam distillation method, distilling for 4 hours, and extracting to obtain 8g of fraction 1 and agilawood residues, wherein the g/ml of distilled material liquid is 1: 2; adding lignum Aquilariae Resinatum residue into 80% ethanol solution, performing ultrasonic extraction at room temperature with ultrasonic frequency of 100KHz for 3 hr, filtering, repeatedly extracting for 5 times, mixing filtrates, and concentrating the filtrate under reduced pressure to relative density of 1.5 to obtain 2L concentrate; extracting the concentrated solution at 25 ℃ for 5 times by using 2L of n-butane as a solvent, combining the extraction solutions, and concentrating the extraction solutions under reduced pressure until no solvent exists to obtain 150 g of fraction 2; mixing fraction 1 and fraction 2 to obtain 158g of lignum Aquilariae Resinatum absolute oil.

The preparation method of the agilawood blasting bead for the cigarette comprises the following steps:

(1) preparing an emulsion: mixing lignum Aquilariae Resinatum absolute oil with pectin and cyclodextrin under stirring to obtain emulsion;

(2) preparing a forming liquid: stirring water, sodium alginate, calcium chloride and Arabic gum together until the mixture is uniformly mixed to obtain a forming solution;

(3) and (3) shaping the dropping pills: and uniformly dripping the emulsion into the forming liquid, curing and forming, cleaning, then putting into a roller, and drying in a rotating manner at the speed of 5r/min at the drying temperature of 30 ℃ and the drying humidity of 40% RH for 3h to obtain the agilawood blasting bead for cigarettes.

Example 4

Example 4 differs from example 1 in that:

the preparation method of the agilawood absolute oil comprises the following steps: crushing 10kg of agilawood to 20 meshes, heating to 100 ℃ by adopting a steam distillation method, distilling for 3 hours, and extracting to obtain 19.5 g of fraction 1 and agilawood residues, wherein the g/ml of distilled material liquid is 1: 3; heating agilawood residues to 70 ℃ by using an ethanol solution with the volume concentration of 75% as a solvent, carrying out reflux extraction for 2 hours, filtering while the residues are hot, repeatedly extracting for 3 times, combining filtrates, and concentrating the filtrate under reduced pressure until no solvent exists to obtain 650 g of fraction 2; the fraction 1 and the fraction 2 were mixed to obtain 669.5 g of agilawood absolute.

Example 5

Example 5 differs from example 1 in that:

the preparation method of the agilawood absolute oil comprises the steps of crushing 10kg of agilawood into 20 meshes, heating to 100 ℃ by adopting a steam distillation method, distilling for 3 hours, and extracting to obtain 19 g of fraction 1 and agilawood residues, wherein the g/ml of distilled material liquid is 1: 3; adding lignum Aquilariae Resinatum residue into 75% ethanol solution, heating to 70 deg.C, reflux extracting for 2 hr, filtering while hot, repeating extracting for 3 times, mixing filtrates, and concentrating the filtrate under reduced pressure to relative density of 1.2 to obtain 6.3L concentrate; extracting the concentrated solution with 6.3L n-butane at 55 deg.C for 5 times, mixing extractive solutions, and concentrating under reduced pressure to no solvent to obtain fraction 2 of 185 g; and mixing the fraction 1 and the fraction 2 uniformly to obtain 204 g of agilawood absolute oil.

Example 6

Example 6 differs from example 1 in that:

in the step (3), the drying is air drying at 20 ℃.

Comparative example 1

In order to compare the action effect of the emulsion formula of the invention and the conventional emulsion formula, the conventional emulsion formula is adopted in the comparative example.

The emulsion comprises the following raw materials in parts by weight: 5g of anhydrous calcium chloride, 1g of sodium carboxymethylcellulose, 2g of cyclodextrin, 5g of poloxamer and 26g of agilawood absolute.

The rest of the procedure was the same as in example 1.

Comparative example 2

In order to compare the action effects of different dosages of the components of the emulsion, the specific dosages of the components of the emulsion in the comparative example are as follows:

the emulsion comprises the following raw materials in parts by weight: 20g of agilawood absolute oil, 0.5g of pectin and 0.5g of cyclodextrin.

The rest of the procedure was the same as in example 1.

Comparative example 3

In order to compare the effects of different dosages of the agilawood absolute, the specific dosages of the agilawood absolute in the comparative example are as follows:

the emulsion comprises the following raw materials in parts by weight: 8g of agilawood absolute oil, 3g of pectin and 2g of cyclodextrin.

The rest of the procedure was the same as in example 1.

Test example 1 component test in Aquilaria agallocha extract

The neat agilawood oils of examples 1-5 were tested by GC-MS under the following specific test conditions, and the test results are shown in table 1.

Mass spectrum conditions: an Electron Impact (EI) ion source; electron energy 70 eV; the ion source temperature is 230 ℃; the temperature of the quadrupole rods is 150 ℃; the interface temperature is 280 ℃; emission current 34.8 muA; multiplier voltage 1561V; the mass scanning range is 20-550 m/z. And searching and checking Nist2005 and Wiley275 standard mass spectrograms of each peak in the total ion flow graph through a mass spectrometric data system, and comparing and identifying the compounds.

Chromatographic conditions are as follows: the chromatographic column is an elastic quartz capillary column HP-5MS 5% Phenyl Methyl Siloxane (30m × 0.25mm × 0.25 μm); temperature rising procedure: keeping the column temperature at 50 ℃ for 2min, heating to 310 ℃ at the speed of 5 ℃/min, and keeping the temperature for 10 min; the temperature of the vaporization chamber is 250 ℃; the carrier gas is high-purity He (99.999%); the carrier gas flow is 1.0 mL/min; no flow diversion; solvent delay time: 3.0 min;

TABLE 1 measurement results of components in the pure lignum Aquilariae Resinatum oil

Sample (I)	Relative content of sesquiterpenes (%)	Relative content of chromone (%)
			Example 1	34.52	51.91
Example 2	25.19	45.69
			Example 3	38.89	52.71
Example 4	12.65	58.15
			Example 5	26.41	39.87

As can be seen from Table 1, the agilawood absolute oil provided by the extraction method provided by the application has the advantages that the relative content of sesquiterpenes is more than 25.19%, the relative content of chromones is more than 45.69%, the relative content of sesquiterpenes and chromones is stable, and the stability of the agilawood absolute oil is ensured; example 4, the relative content of sesquiterpenes in the extracted agilawood essential oil is only 12.65% without n-butane extraction, the extraction temperature of example 5 is too high, the chromone component is cracked due to high temperature, and the relative content of chromone in the extracted agilawood essential oil is only 39.87%.

Test example 2 suction test

The cigarettes prepared by using the exploded beads for the agilawood cigarettes of the examples and the comparative examples are compared with the cigarettes with common exploded beads in the market, the cigarettes are softer (1 poor-5 good), fragrant (1 poor-5 good), broken hand feeling (1 poor-5 good), broken sound (1 poor-5 good), lasting fragrance (1 poor-5 good) and smoking satisfaction (1 poor-5 good) when being put into the mouth, 5 persons in a small group are used for smoking, the scores are averaged, and the smoking results are shown in table 2;

table 2 aspiration test results

Group of	Degree of softness	Fragrance of odor	Lasting aftertaste	Hand feeling of breaking by pinching	Sound of breaking by pinching	Degree of satisfaction of combustion and suction
							Example 1	4.9	4.9	5.0	4.9	4.8	4.9
Example 2	4.7	4.9	5.0	5.0	4.9	4.8
							Example 3	5.0	5.0	5.0	5.0	4.9	5.0
Example 4	4.4	4.5	4.6	4.8	4.8	4.6
							Example 5	4.6	4.6	4.7	4.8	4.8	4.7
Example 6	4.9	4.8	4.9	4.5	4.8	4.7
							Comparative example 1	4.8	4.8	4.3	4.6	4.4	4.5
Comparative example 2	4.9	4.9	4.4	4.5	4.5	4.4
							Comparative example 3	4.6	4.4	4.2	4.8	4.8	4.3
Commercial bead-blasting cigarette 1	3.5	3.8	1.3	4.2	2.5	3.2
							Commercial bead-blasting cigarette 2	2.1	2.5	1.6	2.9	3.5	2.2
Commercial bead-blasting cigarette 3	4.2	4.0	2.5	4.0	4.0	3.8

The results show that: the bead-blasting cigarette for the agilawood cigarette has good agilawood fragrance in smoke, good harmony between the original fragrance of the tobacco and the agilawood fragrance, soft taste, pleasant fragrance, lasting fragrance, good hand-breaking feeling and crisp sound of breaking when being pinched, and can bring relaxation feeling, comfort feeling and satisfaction feeling to consumers; compared with example 1, example 6, which does not adopt the drying method of the present invention, has a poor hand feel of breaking the popping beads; the comparative example 1 adopts a conventional emulsion formula, and the prepared agilawood bead-popping cigarette for cigarettes has poor fragrance durability and smoking satisfaction; comparative example 2 the amount of each component of the emulsion of the invention is not used, and the prepared agilawood cigarette with exploded beads has poor fragrance durability; the content of the agilawood absolute oil used in the comparative example 3 is reduced, and the prepared exploded bead cigarette for the agilawood cigarette has reduced fragrance, softness and lasting fragrance; the overall smoking effect of the commercial popping-bead cigarettes is inferior to the examples.

Test example 3 detection of inhibition of Activity of Lung cancer cell line

The MTT method is adopted to detect the activity of the exploded beads for the agilawood cigarette for inhibiting lung cancer cell strains in the embodiment and the comparative example, the specific detection method is as follows, and the results are shown in Table 3;

1) cell culture: human lung cancer cell strain A549 at 37 deg.C in incubator with 5% CO₂Culturing in complete culture medium containing 10% calf serum RPMI1640 under the condition of humidity greater than 90%; adherent cells were digested with 0.25% pancreatin; cells in logarithmic growth phase were selected for the experiments.

2) The antitumor activity of this example was examined by MTT method, and experimental groups were set up as a test group, a negative control group (commercial ordinary cigarette bead contents, 40. mu.g/mL, 80. mu.g/mL), and a positive control group (mitomycin C solution, 1. mu.g/mL, 5. mu.g/mL, 10. mu.g/mL, 20. mu.g/mL). The test group adopts the contents of the agilawood blasting beads for cigarettes provided by the embodiment of the invention and the comparative example, each sample is provided with 5 concentration gradients, and the concentrations are as follows: 5. mu.g/mL, 10. mu.g/mL, 20. mu.g/mL, 40. mu.g/mL, 80. mu.g/mL; the preparation method of the content of the common cigarette blasting beads and the content of the blasting beads for the agilawood cigarette comprises the steps of adding 2 blasting beads into a test tube, crushing the blasting beads for the cigarette to release the content of the blasting beads, and standing for later use.

3) Selecting cells in logarithmic growth phase, culturing the human lung cancer cell strain A549 cell strain in an RPMI1640 complete culture medium to prepare single cell suspension, counting by using a blood counting chamber, inoculating 90 mu L of the single cell suspension in a 96-hole flat-bottom cell culture plate according to the concentration of 50000 cells/mL, and setting a negative control group, a positive control group and a test group. Adding sample into each cell strain, and culturing at 37 deg.C and 5% CO for 72 hr₂Humidity is above 90%, then the sample is taken out and placed under a microscope to observe the cell shape of each hole;

4) then, 15. mu.L of 5mg/mL MTT solution was added, and after reaction at 37 ℃ for 4 hours, the supernatant was aspirated and removed, 100. mu.L DMSO was added to each well, and the resulting solution was dissolved sufficiently, and the OD value (measurement wavelength: 490nm) of each well was measured by an ELX-800 microplate reader, and the inhibition ratio was calculated according to the following formula:

inhibition (%) - (control OD value-Experimental OD value)/control OD value X100%

5) The concentration of the sample (IC) at 50% inhibition was determined by plotting the sample concentration on the abscissa and the inhibition of the sample on the ordinate₅₀₎The activity results of the samples were expressed as half the Inhibitory Concentration (IC)₅₀) And (4) showing.

TABLE 3 results of the detection of the inhibitory activity of each sample on human lung cancer cell line A549 cells

Note: and/means no inhibitory activity.

As shown in table 3, the exploded beads for agilawood cigarette prepared in examples 1 to 6 and comparative examples 1 to 3 all have a certain cytotoxic activity to lung cancer cell line a549, wherein the amount of the agilawood absolute used in comparative example 3 is not used, the inhibition effect of the prepared exploded beads on the activity of lung cancer cell line a549 is reduced, and the commercially available ordinary exploded beads do not show the inhibition activity. Therefore, the agilawood blasting bead for the cigarette prepared by the agilawood absolute oil has the activity of inhibiting lung cancer cells, and the cigarette prepared by the agilawood blasting bead for the cigarette containing the agilawood absolute oil has the effect of preventing lung cancer.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.