阅读说明：本技术 载体单元以及其制造方法 (Carrier unit and method for manufacturing same ) 是由 杨怡宽 李咏琳 连美蓁 赖秋萍 于 2019-11-29 设计创作，主要内容包括：本发明揭露一种载体单元,其系由环糊精、具有至少二个异氰酸基(-NCO)的化合物以及可与异氰酸基反应的分子单元反应而成,其中环糊精与化合物经交联后,会产生较大的容纳空间以利于装载各种容置分子。再者,交联后的环糊精在与分子单元反应后,使载体单元可同时具有环糊精以及分子单元的特性,以增加载体单元的利用性。载体单元可作为奈米胶囊或奈米海绵,并可应用于多个产业,例如隐形眼镜、涂覆药物贴片、清洁用品或机能服装。本发明亦揭露一种载体单元的制造方法。(The invention discloses a carrier unit, which is formed by the reaction of cyclodextrin, a compound with at least two isocyanate groups (-NCO) and a molecular unit capable of reacting with the isocyanate groups, wherein after the cyclodextrin and the compound are crosslinked, a larger accommodating space is generated to be beneficial to loading various accommodating molecules. Furthermore, after the cross-linked cyclodextrin reacts with the molecular unit, the carrier unit can have the characteristics of both cyclodextrin and the molecular unit, so that the utilization of the carrier unit is increased. The carrier unit can be used as nanocapsules or nanosponges and can be applied in a variety of industries, such as contact lenses, coated drug patches, cleaning supplies or functional garments. The invention also discloses a manufacturing method of the carrier unit.)

1. A carrier element, characterized in that the carrier element has the following general chemical formula:

Ax-By-Cz, wherein x, y, z are each greater than or equal to 1, a is a cyclodextrin, B is a compound having at least a diisocyanato group (-NCO), and C is a molecular unit reactive with an isocyanato group;

wherein one isocyanate group of the compound reacts with a hydroxyl (-OH) group of the cyclodextrin, and another isocyanate group of the compound reacts with the molecular unit or the cyclodextrin to form the carrier unit.

2. The carrier unit of claim 1, wherein the cyclodextrin of the carrier unit reacts with the compound to form a nanocapsule or a nanosponge having a containment space for loading at least one containment molecule.

3. The carrier element of claim 2 wherein said accommodation molecule is a pharmaceutical agent, a detergent, or a chemical agent molecule.

4. The carrier element according to claim 1, wherein the molecular element has at least one functional group for reacting with the compound, the functional group being a hydroxyl group, an amine group (-NH-), a ureylene group (-NHCONH-), a carbamate group (-NHCOO-), a carboxyl group (-COOH), a thiocarboxylic group (-COSH), an epoxy group or an isocyanate group.

5. The carrier unit of claim 1, wherein the carrier unit forms a contact lens, a patch of coated medication, a cleaning article, or a functional garment.

6. The carrier unit of claim 1, wherein the cyclodextrin is an α -cyclodextrin, a β -cyclodextrin, or a γ -cyclodextrin.

7. The carrier element of claim 1 wherein the compound is isophorone diisocyanate (IPDI), Hexamethylene Diisocyanate (HDI) or other compounds having at least a diisocyanato group (-NCO).

8. A method of manufacturing the carrier unit of claim 1, comprising:

step a, mixing the cyclodextrin, the molecular unit and a first solvent to form a first predetermined object;

b, adding and mixing the compound, a second solvent and a surfactant into the first predetermined substance, wherein the adding and mixing actions are finished within 80 minutes, and the reaction is carried out for 2-30 hours at the temperature of 30-70 ℃ during adding and mixing so as to form a second predetermined substance; and

and c, drying the carrier unit prearranged object in the second prearranged object, and removing non-target objects to obtain the carrier unit of the target object.

9. The method of claim 8, wherein in step c, the removing non-target objects further comprises: d, dispersing the dried solid substance into a third solvent to form a third predetermined substance;

step e, centrifuging the third predetermined substance at the rotation speed of 2000-; and

and f, obtaining the centrifuged supernatant of the third predetermined substance and drying to obtain the carrier unit.

10. The process according to claim 8, wherein the compound is isophorone diisocyanate.

11. The method according to claim 8, wherein the molecular unit has at least one functional group to react with the compound, the functional group being a hydroxyl group, an amine group (-NH-), a urea group (-NHCONH-), a urethane group (-NHCOO-), a carboxyl group (-COOH), a thiocarboxylic group (-COSH), an epoxy group or an isocyanate group.

12. The manufacturing method according to claim 8, wherein in steps a, b: the first solvent is water, dimethyl sulfoxide or a solvent which does not react with isocyanate groups and is soluble in water;

the second solvent is water, n-heptane or a solvent that does not react with the first solvent and isocyanate groups; and

the surfactant is a cationic surfactant, an anionic surfactant, a zwitterionic surfactant or a nonionic surfactant.

13. The method of claim 8, wherein in step b: the addition and mixing actions need to be completed within 60 minutes, and the reaction is carried out for 5-24 hours at 45-65 ℃ during the addition and mixing to form the second predetermined substance.

14. The method of manufacturing of claim 9, wherein in step d: the third solvent is water.

15. The method of claim 9, wherein in step e: the third predetermined substance is centrifuged at 2000-.

Technical Field

The present invention relates to a carrier unit and a method for manufacturing the same, and more particularly, to a carrier unit having characteristics of cyclodextrin and a molecular unit, being non-toxic and having high utility, and a method for manufacturing the same.

Background

With the progress and development of medical treatment, the application of nano-carriers (e.g., nanocapsules, nano-sponges) to drug delivery systems is becoming mature. When the medicine is wrapped in the nano-carrier, the dynamic distribution of the medicine can be changed by the characteristics of the nano-carrier, so that the medicine can be effectively sent to the focus, and can be continuously and stably released to control the concentration of the medicine, thereby having the advantage of reducing the side effect of the medicine. Besides the drug delivery (including target drugs) as described above, nanocarriers are widely used in various industries, such as chemical separation and analysis, food processing, cosmetic processing, environmental protection, and the like.

One of the major materials commonly used as nanocarriers is Cyclodextrin (CD), which is a cyclic oligosaccharide extracted from starch after alkaline cooking or enzymatic degradation. Cyclodextrins have good biocompatibility, solubility and no toxicity, and besides being inexpensive, they are also readily available materials, and thus are widely used as research targets for new materials. The three-dimensional structure of cyclodextrin is a truncated cone (also called truncated cone), and the three-dimensional structure enables the annular inner cavity of cyclodextrin to have hydrophobic and oleophilic properties, and the annular outer wall to have hydrophilic properties. The hydrophobic and hydrophilic properties of cyclodextrin allow it to entrap lipophilic molecules within the annular lumen and further control the release of the lipophilic molecules. Besides being used as a nanocarrier directly in the form of a monomer, the cyclodextrin can also be cross-linked to form a larger nanocarrier to increase the space for encapsulating more molecules.

However, the cyclodextrins (including monomeric or crosslinked cyclodextrins) currently used as nanocarriers are generally not immobilized, resulting in high loss rates and difficult reuse, for example, when the cyclodextrins are used in cleaning products, they are discarded after a single adsorption of the contaminants, and even if the adsorbed cyclodextrin is washed for recycling, the loss rate may be increased because it is not immobilized.

In addition, other nano-carriers are made of non-biocompatible materials, for example, a common cleaning product sold in the market, miraculous sponge (or referred to as "scientific sponge") utilizes the pores of the fine fibers in the sponge to absorb grease or dirt, thereby achieving the cleaning effect. However, some of the above-mentioned nanosponges contain chemical substances, such as melamine or formaldehyde, which are harmful to human health, and the long-term use of these nanosponges will cause harm to human health.

Disclosure of Invention

Accordingly, to overcome the deficiencies of the prior art, embodiments of the present invention provide a carrier molecule that combines the properties of cyclodextrin with molecular units. The crosslinked cyclodextrin is immobilized through the molecular unit or imparts the molecular unit character to the carrier molecule. The embodiment of the invention also provides a manufacturing method of the carrier molecule.

In accordance with at least one of the foregoing objects, the present invention provides a carrier unit having the following general chemical formula: Ax-By-Cz, wherein a is a cyclodextrin, B is a compound having at least two isocyanate groups (-NCO), and C is a molecular unit reactive with isocyanate groups, x, y, z are each greater than or equal to 1, wherein one isocyanate group of the compound is reactive with a hydroxyl group of the cyclodextrin, and another isocyanate group of the compound is reactive with the molecular unit or the cyclodextrin to form a carrier unit.

Optionally, the cyclodextrin of the carrier unit reacts with the compound to form nanocapsules or nanosponges having a containment space for loading containment molecules.

Optionally, the accommodation molecule is a medicament, detergent or chemical agent molecule.

Alternatively, the molecular unit has at least one functional group to react with a compound, wherein the functional group is a hydroxyl group, an amine group (-NH-), a carbamide group (-NHCONH-), a carbamate group (-NHCOO-), a carboxyl group (-COOH), a thiocarboxylic group (-COSH), an epoxy group (epoxy), or an isocyanate group.

Optionally, the carrier unit forms a contact lens, a coated drug patch, a cleaning article, or a functional garment.

Optionally, the cyclodextrin is alpha-cyclodextrin, beta-cyclodextrin, or gamma-cyclodextrin.

Alternatively, the compound is isophorone diisocyanate (IPDI), Hexamethylene Diisocyanate (HDI), or other compounds having at least a diisocyanato group (-NCO).

In view of at least one of the above objects, the present invention provides a method for manufacturing the carrier unit, including steps a to c. Step a is mixing the cyclodextrin, the molecular unit, and a first solvent to form a first predetermined species. And step b, adding and mixing the compound, the second solvent and the surfactant into the first predetermined substance within 80 minutes, wherein the adding and mixing actions are completed within 80 minutes, and the second predetermined substance is formed by reacting at the temperature of 30-70 ℃ for 2-30 hours during adding and mixing. Step c is drying the carrier unit in the second predetermined material and removing the non-target material to obtain the carrier unit of the target material.

Optionally, in step c, the method for removing non-target objects further comprises steps d to f. And d, dispersing the dried solid substance into a third solvent to form a third predetermined substance. Step e is centrifugation of the third predetermined material at 2000-. And f, taking supernatant fluid of the centrifuged third predetermined substance and drying to obtain a carrier unit.

Alternatively, the compound is isophorone diisocyanate.

Alternatively, the molecular unit is hydroxyethyl methacrylate (HEMA).

Optionally, in the steps a and b, the first solvent is water, dimethyl sulfoxide (DMSO) or a solvent which does not react with an isocyanate group and is compatible with water, the second solvent is water, n-heptane (heptane) or a solvent which does not react with the first solvent and the isocyanate group, and the surfactant is a cationic surfactant, an anionic surfactant, a zwitterionic surfactant or a nonionic surfactant.

Optionally, in the step b, it is more preferable that the adding and mixing actions are completed within 60 minutes, and the reaction is performed at 45-65 ℃ for 5-24 hours while adding and mixing.

Optionally, in the step d, the third solvent is water.

Optionally, in the step e, it is more preferable that the third predetermined material is centrifuged at 18000 rpm at 2000-.

In short, the carrier unit provided in the experimental example of the present invention has characteristics of both cyclodextrin and a molecular unit, can improve the utility of the carrier unit, and has the advantage of being non-toxic, thus having advantages in various markets where the carrier unit is required (e.g., chemical separation and analysis, food processing, cosmetic processing, environmental protection, etc.).

The foregoing and other objects, features, and advantages of the invention will be apparent from the following more particular description of the invention, as illustrated in the accompanying drawings.

Drawings

FIG. 1A is a schematic representation of the polymeric structure of a carrier unit of one form of embodiment of the present invention.

Fig. 1B is a schematic perspective view of cyclodextrin according to an embodiment of the present invention.

Fig. 2A is a molecular structure diagram of alpha cyclodextrin in an embodiment of the invention.

Fig. 2B is a molecular structure diagram of beta cyclodextrin in an embodiment of the invention.

Fig. 2C is a molecular structure diagram of gamma cyclodextrin according to an embodiment of the present invention.

Fig. 3 is a schematic representation of the general chemical formula Ax-By-Cz of a carrier unit of an embodiment of the invention at x = y = z = 1.

Fig. 4 is a schematic diagram of a nanocapsule according to an embodiment of the present invention.

Fig. 5 is a schematic view of a nanosponging according to an embodiment of the present invention.

Fig. 6 is a flowchart illustrating a method for manufacturing nanocapsules according to an embodiment of the present invention.

Fig. 7A is a table showing the manufacturing conditions and particle size analysis of nanocapsules according to the experimental example of the present invention.

Fig. 7B is a table showing the manufacturing conditions and particle size analysis of nanocapsules according to the experimental example of the present invention.

Fig. 8A is a scanning electron microscope image of nanocapsules of the present invention, which target particle size is 100 nm.

Fig. 8B is a scanning electron microscope image of nanocapsules of 200 nm target particle size according to the experimental example of the present invention.

FIG. 8C is a transmission electron microscope image of nanocapsules of the present invention, wherein the nanocapsules have a target particle size of 100 nm.

FIG. 8D is a transmission electron microscope image of nanocapsules of the present invention, wherein the nanocapsules have a target particle size of 200 nm.

Fig. 9 is a flowchart illustrating a method for manufacturing a nanosponging material according to an embodiment of the present invention.

Fig. 10A is a table showing the manufacturing conditions and particle size analysis of the nano-sponges according to the experimental examples of the present invention.

Fig. 10B is a table showing the manufacturing conditions and particle size analysis of the nano-sponges according to the experimental examples of the present invention.

FIG. 11A is a scanning electron microscope image of a nanosponge with a target particle size of 150 nm according to an example of the present invention.

FIG. 11B is a scanning electron microscope image of a nanosponge with a target particle size of 250 nm according to an example of the present invention.

FIG. 11C is a transmission electron microscope image of a nanosponge with a target particle size of 150 nm according to an example of the present invention.

FIG. 11D is a transmission electron microscope image of a nanosponge with a target particle size of 250 nm according to an example of the present invention.

Wherein:

4: nanocapsules 401: carrier unit

402: the accommodating space 5: nano sponge

501: the carrier unit 502: channel

A: cyclodextrin B: compounds having at least two isocyanate groups

C: molecular units S601 to S606 reactive with isocyanate groups: step (ii) of

S901-S906: and (5) carrying out the following steps.

Detailed Description

In order to fully understand the objects, features and effects of the present invention, the following detailed description of the present invention with reference to the accompanying drawings is provided.

The embodiment of the invention provides a carrier unit, and the chemical general formula of the carrier unit is as follows: Ax-By-Cz, wherein x, y, z are each greater than or equal to 1.

In the above chemical formula, a is cyclodextrin having a plurality of hydroxyl groups (-OH), B is a compound having at least two isocyanate groups (-NCO), and C is a molecular unit reactive with an isocyanate group, wherein one isocyanate group of the compound is reactive with a hydroxyl group of cyclodextrin, and another isocyanate group of the compound is reactive with a molecular unit or with cyclodextrin to form a carrier unit.

Furthermore, after the cross-linked cyclodextrin reacts with the molecular unit, the carrier unit can have the characteristics of both the cyclodextrin and the molecular unit, so as to increase the utilization of the carrier unit. For example, when the carrier unit is applied to a contact lens, the molecular unit may be a molecular unit of the contact lens body, and the carrier unit is the contact lens body (i.e., the contact lens body is connected with the crosslinked cyclodextrin), and the carrier unit (contact lens) can be used for loading a moisturizer or a medicament through the cyclodextrin to increase the wearing comfort or functionality of the contact lens. When the carrier unit is applied to a coated drug patch, the molecular unit can be a molecular unit of a coated drug patch body, the carrier unit is a coated drug patch whole body (namely, the coated drug patch body is connected with the crosslinked cyclodextrin), and the carrier unit (coated drug patch) can be used for loading a medicament through the cyclodextrin to serve as a sustained-release coated drug patch. When the carrier unit is applied to a cleaning product, the molecular unit can be a molecular unit of the cleaning product (such as a cloth cover), and the carrier unit is the whole cleaning product (namely, the cloth cover is connected with the crosslinked cyclodextrin), the carrier unit (the cleaning product) can be used for adsorbing dirt and grease during cleaning through the cyclodextrin, or is used for loading a cleaning agent, and the cyclodextrin is not lost after the cleaning product is cleaned, so that the recycling rate is high. When the carrier unit is applied to a functional garment (e.g., a cooling garment), the molecular unit can be a molecular unit of a functional garment body (e.g., a garment), and the carrier unit is the functional garment whole body (i.e., the garment is connected with the crosslinked cyclodextrin), the carrier unit (functional garment) can be used for loading a chemical agent (e.g., a cooling substance) through the cyclodextrin and regularly releasing the chemical agent, and furthermore, when the cooling substance is released, the functional garment can be loaded with the cooling substance again for recycling. It should be noted that the present invention is not limited by the application target of the carrier unit and the loaded accommodation molecule.

The carrier unit provided By the embodiment of the invention has a chemical formula of Ax-By-Cz, wherein x, y and z are all larger than or equal to 1, and cyclodextrin or molecular unit can react with the compound. Since cyclodextrins have multiple hydroxyl groups that can create cross-linked structures with compounds, the overall result can create polymeric structures with an indefinite ratio of Ax-By-Cz based on the chemical formula (i.e., polymeric structures formed from various possible compositions of y, x, z). As shown in FIG. 1A, which is a schematic diagram of the polymeric structure of the carrier unit in one form of the embodiment of the present invention, other forms of the polymeric structure are not limited to the polymeric structure shown in FIG. 1A. For example, in fig. 1A, the polymeric structure has at least three compositions of y, x, and z, which are { y =1, x =1, z =1}, { y =3, x =3, z =1} and { y =3, x =2, z =1}, respectively.

Next, the carrier unit and the method for manufacturing the same will be further described through examples and test examples. First, referring to fig. 1B, fig. 1B is a schematic perspective view of cyclodextrin according to an embodiment of the present invention. As shown in fig. 1B, cyclodextrin is a cyclic carbohydrate formed by six-membered ring carbohydrate, and its three-dimensional structure is truncated cone (also called truncated cone, round pavilion), wherein the hydroxyl (-OH) of the carbohydrate is divided into primary alcohol and secondary alcohol, the primary alcohol is distributed on the top plane of the truncated cone, and the secondary alcohol is distributed on the bottom plane of the truncated cone. The round table is provided with a hollow annular inner cavity part, and accommodating molecules can be embedded in the hollow annular inner cavity part. Next, referring to fig. 2A-2C, fig. 2A is a molecular structure diagram of α -cyclodextrin, fig. 2B is a molecular structure diagram of β -cyclodextrin, and fig. 2C is a molecular structure diagram of γ -cyclodextrin. As shown in fig. 2A-2C, the common cyclodextrins include three types, i.e., α -cyclodextrin, β -cyclodextrin and γ -cyclodextrin, which have six, seven and eight six-membered saccharides, respectively, and the three dimensional structures form the above-mentioned truncated cone shape.

Next, referring to fig. 3, fig. 3 is a schematic diagram of one case of the general chemical formula Ax-By-Cz of the carrier unit according to the embodiment of the invention, where the general chemical formula is x = y = x = 1. As shown in FIG. 3, the carrier unit is formed by the reaction of cyclodextrin A, compound B and molecular unit C. The structure of cyclodextrin a has a hydroxyl group, the structure of compound B has at least two isocyanate groups (-NCO), and the structure of molecular unit C has a functional group reactive with an isocyanate group, such as, but not limited to, a hydroxyl group, an amine group (-NH-), a thiourea group (-NHCONH-), a carbamate group (-NHCOO-), a carboxyl group (-COOH), a thiocarboxylic group (-COSH), an epoxy group (epoxy), or an isocyanate group. One of the isocyanate groups of compound B reacts with the hydroxyl group of cyclodextrin a and the other isocyanate group of compound B reacts with the functional group of molecular unit C to form the carrier unit. When one of the isocyanate groups of the compound B reacts with the hydroxyl group of the cyclodextrin a, a polymerization reaction is performed, so that the cyclodextrin a is polymerized to form a larger accommodating space for accommodating molecules. Furthermore, the polymerized cyclodextrin a is connected to the molecular unit C through the compound B to form a carrier unit having the characteristics of both cyclodextrin a and the molecular unit C, wherein the cyclodextrin a can be α -cyclodextrin, β -cyclodextrin or γ -cyclodextrin, but the invention is not limited thereto. The carrier unit can be used as nanocapsules or nanosponges and can be applied in a variety of industries such as, but not limited to, chemical separation and analysis, food processing, cosmetic processing, or environmental protection, and the like, and such as, but not limited to, the preparation of contact lenses, the coating of pharmaceutical patches, cleaning supplies, or functional garments.

Next, referring to fig. 4 and 5, fig. 4 is a schematic diagram of a nanocapsule according to an embodiment of the present invention, and fig. 5 is a schematic diagram of a nanosponges according to an embodiment of the present invention. As shown in fig. 4, when the carrier unit is applied to the nanocapsule 4, a micro-sized sphere is formed, the surface of the sphere is the carrier unit 401, and the interior of the sphere has a containing space 402 so that the nanocapsule 4 can be loaded with the containing molecules. As shown in fig. 5, when the carrier unit is applied to the nano sponge 5, a micro-sphere with porous holes is formed, the surface of the sphere is the carrier unit 501, and the interior of the sphere has a plurality of channels 502 formed by connecting the holes in series, so that the nano sponge 5 can be used to load and hold molecules. The difference between the nanocapsule 4 and the nanosponges 5 lies in the different structure of the inner space, the inner space of the nanocapsule 4 is larger and single, and the inner space of the nanosponges 5 is a plurality of small holes connected in series to form a channel, so the usable space of the nanosponges 5 is smaller than that of the nanocapsules 4. The user can select the nanocapsule 4 or the nanosponges 5 for the desired accommodation space of the carrier unit.

Next, referring to fig. 3 and fig. 6, fig. 6 is a flowchart illustrating steps of a method for manufacturing nanocapsules according to an embodiment of the present invention. The nanocapsule according to the embodiment of the present invention is prepared by emulsion polymerization, wherein the cyclodextrin a is β -cyclodextrin having a hydroxyl group, the compound B is isophorone diisocyanate (IPDI) having two isocyanate groups, and the molecular unit C is hydroxyethyl methacrylate (HEMA) having a hydroxyl group and a vinyl group (-C = C), but the present invention is not limited by the types of the cyclodextrin a, the compound B and the molecular unit C, and the compound B may be Hexamethylene Diisocyanate (HDI) or other compounds having at least two isocyanate groups. First, as shown in step S601, beta cyclodextrin and hydroxyethyl methacrylate are added to deionized water as a first solvent and stirred until they are uniformly mixed to form a first predetermined substance, but the present invention is not limited by the type of the first solvent, and it may be any solvent that does not react with isocyanate groups and is compatible with water. Then, as shown in step S602, n-heptane as a second solvent is uniformly mixed with isophorone diisocyanate to form an oil phase solution LC1, and the first predetermined substance is uniformly mixed with Sodium Dodecyl Sulfate (SDS) as an interface active agent to form a clear and transparent aqueous phase solution LC2, and the oil phase solution LC1 is added to and mixed with the aqueous phase solution LC2 to react beta-cyclodextrin, isophorone diisocyanate and hydroxyethyl methacrylate to form a second predetermined substance, wherein the adding and mixing operations are completed within 80 minutes, the temperature for performing the reaction during adding and mixing is controlled to be 30-70 ℃, and the time for performing the reaction after adding and mixing is controlled to be 2-30 hours. More preferably, the oil phase solution LC1 is added and mixed to the water phase solution LC2, wherein the adding and mixing actions are completed within 60 minutes, the temperature for reaction after adding and mixing is controlled to be 45-65 ℃, and the time for reaction during adding and mixing is controlled to be 5-24 hours. It should be noted that the present invention is not limited by the type of the second solvent and the surfactant, the second solvent may be any solvent that does not react with the first solvent and the isocyanate group, and the surfactant may be other cationic surfactants, anionic surfactants, zwitterionic surfactants or nonionic surfactants. Then, as shown in step S603, the second predetermined object is dried and the non-target object is removed, so that the carrier unit of the target object can be finally obtained. In the embodiment of the present invention, the carrier unit predetermined substance LC3 in the second predetermined substance is put into an oven for standing and drying, and after drying, the carrier unit predetermined substance LC3 is obtained as white powder, but the present invention is not limited to the drying method. Next, please refer to steps S604-S606 for a method of removing non-target objects. As shown in step S604, the carrier unit reservation LC3 is dispersed into water as a third solvent and shaken to configure a third reservation LC 4. Next, as shown in step S605, the rotation speed of the centrifuge was set to 2000-. More preferably, the rotation speed of the centrifuge is set to 5000-. Finally, in step S606, the supernatant of the third predetermined substance LC4 is obtained and dried to obtain the nanocapsule-forming carrier unit. It should be noted that the present invention is not limited to the method of removing the non-target substance. In the embodiment of the present invention, the nanocapsule prepared from beta cyclodextrin, isophorone diisocyanate and hydroxyethyl methacrylate (with vinyl group) has beta cyclodextrin and vinyl group and its characteristics, the vinyl group of the nanocapsule can perform a cross-linking reaction with the raw material of the contact lens to form the contact lens, and the nanocapsule can further encapsulate a containing molecule (e.g., a moisturizing liquid or a medicament), but the present invention is not limited to the product formed by the nanocapsule and the encapsulated containing molecule.

In steps S602 and S605 of fig. 6, the present invention prepares the carrier unit as nanocapsules under six different conditions, please refer to fig. 7A and 7B, and fig. 7A and 7B are tables of manufacturing conditions and particle size analysis of nanocapsules in the experimental example of the present invention. As shown in FIGS. 7A and 7B, in test example 1, the reaction temperature in step S602 was controlled to 45 degrees, the reaction time was controlled to 5 hours, and the centrifugation rotation speed in step S605 was controlled to 10000-. In test example 2, the reaction temperature in step S602 was controlled to 45 degrees, the reaction time was controlled to 24 hours, and the centrifugal rotation speed in step S605 was controlled to 10000- & ltSUB & gt 15000 rpm, and the centrifugal time was controlled to at least 1 hour, and a carrier unit having an average particle diameter of 99.10 nm was obtained, in which the particle diameter analysis chart (X-axis: diameter (nm); Y-axis: quantity (%)) exhibited a Gaussian distribution. In test example 3, the reaction temperature in step S602 was controlled to 65 degrees, the reaction time was controlled to 24 hours, and the centrifugation rotation speed in step S605 was controlled to 10000- & gt 15000 rpm, and the centrifugation time was controlled to at least 1 hour, and a carrier unit having an average particle diameter of 102.80 nm was obtained, in which the particle diameter analysis chart (X-axis: diameter (nm); Y-axis: quantity (%)) exhibited a Gaussian distribution. In test example 4, the reaction temperature in step S602 was controlled to 45 degrees, the reaction time was controlled to 5 hours, and the centrifugal rotation speed in step S605 was controlled to 5000-. In test example 5, the reaction temperature in step S602 was controlled to 45 degrees, the reaction time was controlled to 24 hours, and the centrifugal rotation speed in step S605 was controlled to 5000-. In test example 6, the reaction temperature in step S602 was controlled to 65 degrees, the reaction time was controlled to 24 hours, and the centrifugal rotation speed in step S605 was controlled to 5000-.

The nanocapsules prepared in the test examples 1-6 of the present invention can be observed by Scanning Electron Microscope (SEM) and Transmission Electron Microscope (SEM) for morphology and particle size. Referring to fig. 8A to 8D, fig. 8A is a scanning electron microscope image of nanocapsules of 100 nm target particle size according to the experimental example of the present invention, fig. 8B is a scanning electron microscope image of nanocapsules of 200 nm target particle size according to the experimental example of the present invention, fig. 8C is a transmission electron microscope image of nanocapsules of 100 nm target particle size according to the experimental example of the present invention, and fig. 8D is a transmission electron microscope image of nanocapsules of 200 nm target particle size according to the experimental example of the present invention. As shown in fig. 8A-8D, the nanocapsule is a hollow cavity under a transmission electron microscope.

Next, referring to fig. 3 and fig. 9, fig. 9 is a flowchart illustrating steps of a method for manufacturing a nano sponge according to an embodiment of the invention. The nanosponges of the embodiments of the present invention are prepared by emulsion polymerization, wherein the cyclodextrin a is beta cyclodextrin having hydroxyl group, the compound B is isophorone diisocyanate having two isocyanate groups, and the molecular unit C is hydroxyethyl methacrylate having hydroxyl group and vinyl group, but the invention is not limited by the types of the cyclodextrin a, the compound B and the molecular unit C, for example, the compound B may also be hexamethylene diisocyanate or other compounds having at least two isocyanate groups. First, as shown in step S901, beta cyclodextrin, hydroxyethyl methacrylate and dimethyl sulfoxide (DMSO) as a first solvent are uniformly mixed to form a first predetermined substance, but the present invention is not limited to the type of the first solvent, and may be any solvent that does not react with an isocyanate group and is compatible with water. Then, as shown in step S902, uniformly mixing the first predetermined material with isophorone diisocyanate to form a dispersed phase solution LS1, and uniformly mixing water as a second solvent with sodium dodecyl sulfate as a surfactant to form a continuous phase solution LS2, adding and mixing the dispersed phase solution LS1 to the continuous phase solution LS2 to react beta-cyclodextrin, isophorone diisocyanate and hydroxyethyl methacrylate to form a second predetermined material, wherein the time for adding and mixing is required to be completed within 80 minutes, the temperature for performing the reaction during adding and mixing is controlled to be 30-70 ℃, and the time for performing the reaction during adding and mixing is controlled to be 2-30 hours. More preferably, the dispersed phase solution LS1 is mixed with the continuous phase solution LS2 within 60 minutes, the reaction temperature is controlled to be 45-65 ℃, and the reaction time is controlled to be 5-24 hours. It should be noted that the present invention is not limited by the type of the second solvent and the surfactant, the second solvent may be any solvent that does not react with the first solvent and the isocyanate group, and the surfactant may be other cationic surfactants, anionic surfactants, zwitterionic surfactants or nonionic surfactants. Then, as shown in step S903, the second predetermined object is dried and the non-target object is removed, so that the carrier unit of the target object can be finally obtained. In the embodiment of the present invention, the drying method is to add the second predetermined material into water, centrifuge at 18000 rpm for at least 1 hour to separate solid and liquid, then put the solid carrier unit predetermined material LC3 into the oven to be left for drying, and obtain the carrier unit predetermined material LS3 as white powder after drying, but the present invention is not limited by the drying method. Next, please refer to steps S904-S906 for a method of removing non-target objects. As shown in step S904, the carrier unit reservation LS3 was dispersed into water as a third solvent and shaken to configure a third reservation LS 4. Next, as shown in step S905, the rotation speed of the centrifuge is set to 2000-. More preferably, the rotation speed of the centrifuge is set to 5000-. Finally, in step S906, the supernatant of the third predetermined substance LS4 is obtained and dried to obtain the carrier unit forming the nano-sponge. It should be noted that the present invention is not limited to the method of removing the non-target substance. In the embodiment of the present invention, the nano sponge prepared from beta cyclodextrin, isophorone diisocyanate and hydroxyethyl methacrylate (with vinyl groups) has beta cyclodextrin, vinyl groups and their characteristics, and the vinyl groups of the nano sponge can undergo a cross-linking reaction with the raw materials of the contact lens to form the contact lens and further wrap the receiving molecules (e.g., the moisturizing solution or the medicament), but the present invention is not limited to the product formed by the nano sponge and the wrapped receiving molecules.

In steps S902 and S905 of fig. 9, the carrier unit as a nano sponge is prepared under six different conditions, please refer to fig. 10A and 10B, and fig. 10A and 10B are a table of the manufacturing conditions and particle size analysis of the nano sponge in the experimental example of the present invention. As shown in FIGS. 10A and 10B, in test example 7, the reaction temperature in step S902 was controlled to 45 degrees, the reaction time was controlled to 5 hours, and the centrifugal rotation speed in step S905 was controlled to 10000-. In test example 8, the reaction temperature in step S902 was controlled to 45 degrees, the reaction time was controlled to 24 hours, and the centrifugal rotation speed in step S905 was controlled to 10000- & ltSUB & gt 15000 rpm, and the centrifugal time was controlled to at least 1 hour, whereby a carrier unit having an average particle diameter of 143.40 nm was obtained, in which the particle diameter analysis chart (X-axis: diameter (nm); Y-axis: quantity (%)) exhibited a Gaussian distribution. In test example 9, the reaction temperature in step S902 was controlled to 65 degrees, the reaction time was controlled to 24 hours, and the centrifugal rotation speed in step S905 was controlled to 10000- & ltSUB & gt 15000 rpm, and the centrifugal time was controlled to at least 1 hour, a carrier unit having an average particle diameter of 143.20 nm was obtained, in which the particle diameter analysis chart (X-axis: diameter (nm); Y-axis: quantity (%)) exhibited a Gaussian distribution. In test example 10, the reaction temperature in step S902 was controlled to 45 degrees, the reaction time was controlled to 5 hours, and the centrifugal rotation speed in step S905 was controlled to 5000-10000 rpm, and the centrifugal time was controlled to at least 1 hour, so that a carrier unit having an average particle diameter of 247.20 nm was obtained, in which the particle diameter analysis chart (X-axis: diameter (nm); Y-axis: quantity (%)) exhibited a Gaussian distribution. In test example 11, the reaction temperature in step S902 was controlled to 45 degrees, the reaction time was controlled to 24 hours, and the centrifugal rotation speed in step S605 was controlled to 5000-. In test example 12, the reaction temperature in step S902 was controlled to 65 degrees, the reaction time was controlled to 24 hours, and the centrifugal rotation speed in step S905 was controlled to 5000-10000 rpm, and the centrifugal time was controlled to at least 1 hour, so that a carrier unit having an average particle diameter of 254.60 nm was obtained, in which the particle diameter analysis chart (X-axis: diameter (nm); Y-axis: quantity (%)) exhibited a Gaussian distribution.

The nano-sponges prepared in the experimental examples 7-12 of the present invention were observed for morphology and particle size by using a scanning electron microscope and a transmission electron microscope. Referring to fig. 11A to 11D, fig. 11A is a scanning electron microscope image of a nano sponge having a target particle size of 150 nm according to an experimental example of the present invention, fig. 11B is a scanning electron microscope image of a nano sponge having a target particle size of 250 nm according to an experimental example of the present invention, fig. 11C is a transmission electron microscope image of a nano sponge having a target particle size of 150 nm according to an experimental example of the present invention, and fig. 11D is a transmission electron microscope image of a nano sponge having a target particle size of 250 nm according to an experimental example of the present invention. As shown in fig. 11A-11D, the nanosponges appear porous under the transmission electron microscope.

The nanocapsules and the nanosponges are all nano materials with three-dimensional network structures, the nanocapsules and the nanosponges not only keep the inner layer of the cyclodextrin relative to the hydrophobic cavity, but also can form the outer layer of the cyclodextrin relative to the hydrophilic cavity in the three-dimensional network structure in the cross-linking and preparation processes, and the two layers of cavities with different properties can increase the application elasticity of the nanocapsules and the nanosponges.

In view of the above, the technical effects of the carrier unit and the manufacturing method thereof according to the embodiments and the test examples of the present invention are described below in comparison with the prior art.

In the prior art, part of the carrier units cannot be fixed, so that the loss rate is high, and the carrier units cannot be effectively reused, and the components of the carrier units are toxic, so that the carrier units can cause harm when being used for organisms. In contrast to the carrier units and methods of making the same according to embodiments of the present invention, which are non-toxic and have the properties of both cyclodextrin and molecular units, the carrier units are highly available (e.g., the carrier units can be bound to other products, such as contact lenses, via the molecular units), and are sufficiently competitive in a variety of industries (e.g., contact lenses, coated drug patches, cleaning products, or functional garments).

The present invention has been disclosed in the foregoing in terms of preferred embodiments, but it should be understood by those skilled in the art that the above-described embodiments are merely illustrative of the present invention and should not be construed as limiting the scope of the present invention. It should be noted that all the equivalent changes and substitutions to the above-mentioned experimental examples should be considered to be covered by the scope of the present invention.