阅读说明：本技术 工程化糖基转移酶和甜菊醇糖苷葡糖基化方法 (Engineering glycosyltransferases and methods of glycosylation of steviol glycosides ) 是由 乔纳森·弗罗姆 斯蒂芬妮·苏·加兰妮 杰克·梁 刘至中 尼基·德拉斯 美利莎·安·梅奥 于 2019-07-17 设计创作，主要内容包括：本发明提供了工程化糖基转移酶(GT)、具有GT活性的多肽和编码这些酶的多核苷酸,以及包含这些多核苷酸和多肽的载体和宿主细胞。本发明提供了工程化蔗糖合酶(SuS)、具有SuS活性的多肽和编码这些酶的多核苷酸,以及包含这些多核苷酸和多肽的载体和宿主细胞。本发明还提供了包含GT酶的组合物和使用工程化GT酶来制备具有β-葡萄糖连接的产物的方法。本发明还提供了用于产生莱鲍迪苷(rebaudiosides)(例如莱鲍迪苷M、莱鲍迪苷A、莱鲍迪苷I和莱鲍迪苷D)的组合物和方法。本发明还提供了包含SuS酶的组合物及使用它们的方法。还提供了用于产生GT酶和SuS酶的方法。(The invention provides engineered Glycosyltransferases (GT), polypeptides having GT activity and polynucleotides encoding the enzymes, as well as vectors and host cells comprising the polynucleotides and polypeptides. The present invention provides engineered sucrose synthases (SuS), polypeptides having SuS activity and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The invention also provides compositions comprising the GT enzyme and methods of using the engineered GT enzyme to prepare products having β -glucose linkages. The present invention also provides compositions and methods for producing rebaudiosides, such as rebaudioside M, rebaudioside a, rebaudioside I, and rebaudioside D. The invention also provides compositions comprising SuS enzymes and methods of using them. Methods for producing the GT and SuS enzymes are also provided.)