阅读说明：本技术 一种含有园艺黄荆种子的生根剂 (Rooting agent containing garden negundo chastetree seeds ) 是由 周德超 于 2019-10-29 设计创作，主要内容包括：本发明公开一种含有园艺黄荆种子的生根剂,其原料包括：园艺黄荆种子粉、薄荷根粉、鱼腥草粉、茅草根粉、花叶芦竹根粉、荷兰菊粉、六棱菊粉、马尾伸筋草粉、美丽月见草提取液、刺黄莲提取液、麦草粉、落叶松提取液、苍耳提取液、马齿苋提取液。本发明能够提高植物生根剂的综合效果,且对园艺黄荆种子的新用途具有指导性意义。(The invention discloses a rooting agent containing garden negundo chastetree seeds, which comprises the following raw materials: horticultural negundo chastetree seed powder, mint root powder, houttuynia cordata powder, couch grass root powder, floral leaf giant reed root powder, dutchmanspipe flower powder, six-arrier chrysanthemum powder, horsetail lycopodium clavatum powder, beautiful evening primrose extract, yellow lotus extract, wheat grass powder, larch extract, xanthium sibiricum extract and purslane extract. The invention can improve the comprehensive effect of the plant rooting agent and has instructive significance for the new application of the horticultural negundo chastetree seeds.)

1. A rooting agent containing garden negundo chastetree seeds is characterized in that the preparation method of the rooting agent comprises the following steps:

s1, weighing 30-50 parts of garden negundo chastetree seed powder, 15-25 parts of mint root powder, 5-7 parts of houttuynia cordata powder, 5-9 parts of couch grass root powder, 1-10 parts of giant reed rhizome powder, 4-8 parts of dutchmanspipe inulin, 2-8 parts of six-edge chrysanthemum powder, 3-5 parts of horsetail lycopodium clavatum powder, 3-7 parts of beautiful evening primrose extract, 5-15 parts of yellow lotus extract, 0.5-1.5 parts of wheat grass powder, 0.2-0.8 part of larch extract, 0.6-0.8 part of xanthium sibiricum extract and 1.2-1.8 parts of purslane extract according to the mass part ratio;

s2, sterilizing and drying garden negundo chastetree seed powder, mint root powder, houttuynia cordata powder, couch grass root powder, floral leaf giant reed root powder and dutchmanspipe flower powder, putting into a reaction kettle with a thermometer, a heating device and a stirring device, stirring for 10-30min at 35-45 ℃, stirring at the speed of 220-250r/min, heating to 50-60 ℃, adding six-edged chrysanthemum powder, common clubmoss herb powder, evening primrose extract and yellow lotus extract, continuously stirring for 15-35min, and stirring at the speed of 220-230 r/min;

s3, heating to 65-75 ℃, adding the wheat straw powder, the larch extracting solution, the xanthium sibiricum extracting solution and the purslane extracting solution, stirring for 25-40min at the stirring speed of 210-;

and S4, putting the mixture into a ball mill after complete stirring, carrying out ball milling for 30-50min, filtering, sterilizing and filling.

2. The rooting agent containing the horticultural negundo chastetree seeds as claimed in claim 1, is characterized in that the raw materials comprise the following components in parts by mass: 35-45 parts of garden negundo chastetree seed powder, 18-23 parts of mint root powder, 5.5-6 parts of houttuynia cordata powder, 6-8 parts of couch grass root powder, 3-8 parts of floral leaf giant reed root powder, 5-7 parts of dutchmanspipe flower powder, 4-7 parts of six-edge chrysanthemum powder, 3.5-4.5 parts of common clubmoss herb powder, 4-6 parts of beautiful evening primrose extract, 8-12 parts of yellow lotus extract, 0.7-1 part of wheat grass powder, 0.4-0.6 part of larch extract, 0.65-0.75 part of xanthium extract and 1.4-1.6 parts of purslane extract.

3. The rooting agent containing the horticultural negundo chastetree seeds as claimed in claim 1, is characterized in that the raw materials comprise the following components in parts by mass: 40 parts of garden negundo chastetree seed powder, 20 parts of mint root powder, 6 parts of houttuynia cordata powder, 6 parts of couch grass root powder, 5 parts of floral leaf giant reed root powder, 6 parts of dutchmanspipe flower powder, 5 parts of six-edged chrysanthemum powder, 4 parts of horsetail lycopodium clavatum powder, 5 parts of beautiful evening primrose extract, 10 parts of yellow thorn lotus extract, 1 part of wheat straw powder, 0.5 part of larch extract, 0.7 part of xanthium sibiricum extract and 1.5 parts of purslane extract.

4. The rooting agent containing the horticultural negundo chastetree seeds as claimed in claim 1, is characterized in that the raw materials comprise the following components in parts by mass: 45 parts of horticultural negundo chastetree seed powder, 21 parts of mint root powder, 5.5 parts of houttuynia cordata powder, 7 parts of couchgrass root powder, 8 parts of floral leaf giant reed root powder, 7 parts of dutchmanspipe flower powder, 6 parts of six-edged chrysanthemum powder, 4.5 parts of common clubmoss herb powder, 6 parts of beautiful evening primrose herb extracting solution, 9 parts of yellow thorn lotus extracting solution, 1.2 parts of wheat grass powder, 0.6 part of larch extracting solution, 0.65 part of siberian cocklebur extracting solution and 1.4 parts of purslane extracting solution.

5. The rooting agent containing the seeds of horticultural negundo chastetree according to claim 1 or 2, wherein S4 is sterilized with ozone.

Technical Field

The invention relates to the technical field of gardening negundo chastetree fruits, in particular to a rooting agent containing gardening negundo chastetree fruit seeds.

Background

The horticultural vitex negundo is an excellent variety in vitex agnus-castus, is a small deciduous tree or shrub plant of vitex of Verbenaceae, and has fragrance throughout the plant. Square small branches, dense grey down. The leaves are opposite, palm-shaped to form compound leaves, the edges of the minor leaves are provided with a plurality of saw teeth, the upper part is green, the lower part is light green, and no hair or slight hair exists. The top of the cone-shaped inflorescence is large and bell-shaped, the top has a crown with split teeth which is purplish blue and bright, and the top has a lobe. The fruit is spherical, yellow brown to dark brown, is similar to black pepper seed in shape, has spicy fragrance, and is used as a seasoning in some areas. The flowering period is 6-10 months, and the flowering period in summer and autumn is positive, so that the aromatic plants are strong in ornamental value. Vitex agnus-castus is distributed in temperate or subtropical regions, including the middle part of Asia, the southern Europe, the Mediterranean region and the like, particularly the southern Turkish region has rich Vitex agnus-castus resources, and at present, some regions of China are introduced, such as Jiangsu, Zhejiang, Shanghai, Hubei, Chongqing, Sichuan, Anhui and the like.

Vitex agnus-castus is one of the commonly used herbs in Europe, and its therapeutic effect on diseases has been recognized in the United states, Germany, Japan, and other countries. Vitex agnus-castus fruits were documented as early as 2500 years ago to be used for the treatment of gynecological disorders such as dysmenorrhea, irregular menstruation, etc., and were said to be commonly used by herders and churches to reduce libido. In addition, since ancient Greece, the fruit has been used as a botanical drug for women's health. In turkey, residents use Vitex agnus-castus leaves to treat abdominal pain, fruits are used for diuresis, dispelling wind, and sedation, while flowers, leaves, and rhizomes are used to treat menstrual cycle disorders; in brazil, vitex agnus-castus is used for religious ceremony and park ornamental plants, and its twigs and fruits are also used for treating stomachache, headache, syphilis, etc.; in Iran folk, Vitex agnus-castus is used to treat milk disorders, convulsions, epilepsy, constipation, etc. Along with the continuous and intensive research on Vitex agnus-castus by foreign scholars, Vitex agnus-castus is gradually discovered to have estrogen-like effect and good curative effect on female diseases, Vitex agnus-castus fruit extract is approved to be used for treating irregular menstruation, premenstrual syndrome and mastalgia in Germany in 1992, and Vitex agnus-castus fruit is loaded into European pharmacopoeia to enable the Vitex agnus-castus fruit to have clear medicinal standards. Vitex agnus-castus contains numerous chemical components with biological activity. Casticin is the main chemical component and the characteristic component of casticin, and the content of casticin in casticin fruits is not less than 0.8 percent and can exert various biological activities such as anti-inflammation, anti-tumor, prolactin secretion inhibition and the like according to the regulation of European pharmacopoeia. The Vitex agnus-castus also contains abundant volatile oil components, mainly including cineole, myrtenol, pinene, sabinene, farnesene, geranene, caryophyllene and alpha-terpinyl acetate; flavones such as apigenin, luteolin, kaempferol-3-O-rutinoside, isoluteolin 6-c- (2-O-trans-caffeoyl) glucoside, vitexin and isoorientin; phenolic acids such as hydroxybenzoic acid, 3, 4-dihydroxybenzoic acid, and the like. In addition, carotenoid, chlorogenic acid, isochlorogenic acid, etc. are contained, and has antioxidant effect.

The research on the garden vitex negundo is mainly focused on the research on the medicinal value of the garden vitex negundo. The research shows that the horticultural vitex negundo seeds have potential plant rooting promoting effect in the plant cuttage process, and the horticultural vitex negundo seeds are used as raw material components of the rooting agent or can obtain unexpected effect.

Disclosure of Invention

The purpose of the invention is as follows: the invention aims to provide a rooting agent containing garden negundo chastetree seeds for overcoming the defects of the prior art, so as to improve the comprehensive effect of the plant rooting agent and have instructive significance for new application of the garden negundo chastetree seeds.

The technical scheme is as follows: the invention relates to a rooting agent containing garden negundo chastetree seeds, and a preparation method of the rooting agent comprises the following steps:

s1, weighing 30-50 parts of garden negundo chastetree seed powder, 15-25 parts of mint root powder, 5-7 parts of houttuynia cordata powder, 5-9 parts of couch grass root powder, 1-10 parts of giant reed rhizome powder, 4-8 parts of dutchmanspipe inulin, 2-8 parts of six-edge chrysanthemum powder, 3-5 parts of horsetail lycopodium clavatum powder, 3-7 parts of beautiful evening primrose extract, 5-15 parts of yellow lotus extract, 0.5-1.5 parts of wheat grass powder, 0.2-0.8 part of larch extract, 0.6-0.8 part of xanthium sibiricum extract and 1.2-1.8 parts of purslane extract according to the mass part ratio;

s2, sterilizing and drying garden negundo chastetree seed powder, mint root powder, houttuynia cordata powder, couch grass root powder, floral leaf giant reed root powder and dutchmanspipe flower powder, putting into a reaction kettle with a thermometer, a heating device and a stirring device, stirring for 10-30min at 35-45 ℃, stirring at the speed of 220-250r/min, heating to 50-60 ℃, adding six-edged chrysanthemum powder, common clubmoss herb powder, evening primrose extract and yellow lotus extract, continuously stirring for 15-35min, and stirring at the speed of 220-230 r/min;

s3, heating to 65-75 ℃, adding the wheat straw powder, the larch extracting solution, the xanthium sibiricum extracting solution and the purslane extracting solution, stirring for 25-40min at the stirring speed of 210-;

and S4, putting the mixture into a ball mill after complete stirring, carrying out ball milling for 30-50min, filtering, sterilizing and filling.

Preferably, the raw materials comprise the following components in parts by weight: 35-45 parts of garden negundo chastetree seed powder, 18-23 parts of mint root powder, 5.5-6 parts of houttuynia cordata powder, 6-8 parts of couch grass root powder, 3-8 parts of floral leaf giant reed root powder, 5-7 parts of dutchmanspipe flower powder, 4-7 parts of six-edge chrysanthemum powder, 3.5-4.5 parts of common clubmoss herb powder, 4-6 parts of beautiful evening primrose extract, 8-12 parts of yellow lotus extract, 0.7-1 part of wheat grass powder, 0.4-0.6 part of larch extract, 0.65-0.75 part of xanthium extract and 1.4-1.6 parts of purslane extract.

Preferably, the raw materials comprise the following components in parts by weight: 40 parts of garden negundo chastetree seed powder, 20 parts of mint root powder, 6 parts of houttuynia cordata powder, 6 parts of couch grass root powder, 5 parts of floral leaf giant reed root powder, 6 parts of dutchmanspipe flower powder, 5 parts of six-edged chrysanthemum powder, 4 parts of horsetail lycopodium clavatum powder, 5 parts of beautiful evening primrose extract, 10 parts of yellow thorn lotus extract, 1 part of wheat straw powder, 0.5 part of larch extract, 0.7 part of xanthium sibiricum extract and 1.5 parts of purslane extract.

Preferably, the raw materials comprise the following components in parts by weight: 45 parts of horticultural negundo chastetree seed powder, 21 parts of mint root powder, 5.5 parts of houttuynia cordata powder, 7 parts of couchgrass root powder, 8 parts of floral leaf giant reed root powder, 7 parts of dutchmanspipe flower powder, 6 parts of six-edged chrysanthemum powder, 4.5 parts of common clubmoss herb powder, 6 parts of beautiful evening primrose herb extracting solution, 9 parts of yellow thorn lotus extracting solution, 1.2 parts of wheat grass powder, 0.6 part of larch extracting solution, 0.65 part of siberian cocklebur extracting solution and 1.4 parts of purslane extracting solution.

Preferably, in S4, sterilization is performed with ozone.

Compared with the prior art, the invention has the beneficial effects that: the invention has wide raw material source, low cost, 1-2 years of storage period, no environmental pollution, no blockage of river channels and simple configuration. The invention takes the horticultural negundo chastetree seeds as the main component, grinds the negundo chastetree seeds into powder, fully cooperates with the rest powder components, has sufficient active nutrient substances, improves the activity of SOD in the cutting seedlings, ensures that the root systems of the cutting seedlings grow well, reduces the morbidity of bacterial leaf rot, leaf spot and ear root rot, ensures that the morbidity of root rot, root tumor and rust disease is 0 percent, and simultaneously can improve the cold resistance, the reproduction rate, the survival rate and the rooting rate of the cutting seedlings and the quality of the cutting seedlings and form callus. The components of the invention generate synergistic effect, can be widely used in various environments, has simple process, can be widely produced and continuously replaces the existing materials.

Detailed Description

The technical solution of the present invention is described in detail by the following specific examples, but the scope of the present invention is not limited to the examples.

Example 1

A rooting agent containing garden negundo chastetree seeds is prepared by the following steps:

s1, weighing 30 parts of garden vitex negundo seed powder, 25 parts of mint root powder, 5 parts of houttuynia cordata powder, 5 parts of couch grass root powder, 10 parts of floral leaf donax root powder, 4 parts of dutchmanspipe flower powder, 8 parts of six-edge chrysanthemum powder, 3 parts of Chinese red clubmoss herb powder, 7 parts of beautiful evening primrose extract, 5 parts of yellow meadowrue extract, 1.5 parts of wheat grass powder, 0.2 part of larch extract, 0.8 part of xanthium sibiricum extract and 1.2 parts of purslane extract according to the mass part ratio of the components;

s2, sterilizing and drying garden vitex negundo seed powder, mint root powder, houttuynia cordata powder, couch grass root powder, floral leaf giant reed root powder and dutchmanspipe flower powder, putting into a reaction kettle with a thermometer, a heating device and a stirring device, stirring for 30min at 35 ℃, stirring at the speed of 220r/min, heating to 60 ℃, adding six-arrier chrysanthemum powder, common clubmoss herb powder, beautiful evening primrose extract and yellow lotus extract, and continuing stirring for 15min at the stirring speed of 230 r/min;

s3, heating to 65 ℃, adding wheat straw powder, larch extracting solution, xanthium sibiricum extracting solution and purslane extracting solution, and stirring for 40min at the stirring speed of 210 r/min;

and S4, stirring completely, putting into a ball mill, ball-milling for 30min, filtering, sterilizing with ozone, and filling.

The raw materials of the embodiment are widely available, the cost is low, the storage period is 2 years, the environment cannot be polluted, the river channel cannot be blocked, and the configuration is simple. The invention takes the horticultural negundo chastetree seeds as the main component, grinds the negundo chastetree seeds into powder, fully cooperates with the rest powder components, has sufficient active nutrient substances, improves the activity of SOD in the cutting seedlings, ensures that the root systems of the cutting seedlings grow well, reduces the morbidity of bacterial leaf rot, leaf spot and ear root rot, ensures that the morbidity of root rot, root tumor and rust disease is 0 percent, and simultaneously can improve the cold resistance, the reproduction rate, the survival rate and the rooting rate of the cutting seedlings and the quality of the cutting seedlings and form callus.

The test is carried out in No. 6 greenhouse of new No. 15 test field of Nanjing perennial root botanical garden (east longitude 118 degrees 21 '-118 degrees 46', north latitude 30 degrees 51 '-32 degrees 15'), the greenhouse is covered with 80-90% of sunshade net, the temperature in the greenhouse is 25 + -2 ℃, and the humidity is 80-90%. The land belongs to subtropical monsoon climate, has large difference between the annual seasons, obvious abundance and darkness, uneven distribution of rainfall, annual average temperature of 15.4 ℃, 39.7 ℃ and extremely low temperature of-13.1 ℃.

Branches of the mother plants of the perennial carnation, the purple coneflower and the lagerstroemia indica which grow vigorously, develop fully and have no diseases and insect pests are collected at 7 am to be used as cutting slips. And spraying water on the collected spike strips by using a spray-shaped spraying pot, and then bringing the spike strips back to the greenhouse for treatment. Cutting the ear strips into 7-10cm long, reserving 2 leaves at the top, cutting off the leaves 1/2-2/3, cutting the upper end horizontally, and cutting the lower end obliquely at a position 0.5cm below the knot. The cut ear strips are divided into 12 groups according to 36 groups. The rooting agent was the product of example 1 and IBA. When the scion is treated, the scion which is cut is respectively soaked in a rooting agent for 10min, and then the scion is naturally dried in the shade. Inserting the treated scion wood into peat soil sterilized by 1000 times of carbendazim: the volume ratio of perlite is 1: 3, after the cutting, watering thoroughly to ensure that the cutting slips are fully contacted with the matrix. Finally, pouring the mixture once by 1000 times of carbendazim.

Watering is carried out according to the temperature and humidity in the greenhouse and the moisture condition of the matrix, so that the temperature in the greenhouse is kept at 20-25 ℃, the humidity is kept above 80%, the watering amount in the first week is large, and the watering amount is reduced when the spike sprouts, so as to ensure the normal development of the root system. Pouring 1000 times of carbendazim every other week, frequently observing the condition and timely removing withered and fallen leaves.

Example 1 and IBA had different effects on the rooting of carnation, echinacea and lagerstroemia indica cuttings. As can be seen from Table 1, both the example 1 and IBA can effectively improve the cuttage rooting rate of the ratoon carnation, the echinacea purpurea and the lagerstroemia indica flower, and the product can replace chemical reagents and is more environment-friendly.

TABLE 1 Effect of hormones on cuttage rooting of Phyllostachys perennialis, Echinacea purpurea and Lagerstroemia indica

Treatment of	Average rooting percentage/%)
		Example 1 treatment of Phyllostachys perennis	93.52
EXAMPLE 1 treatment of Echinacea purpurea	94.78
		Example 1 treatment of Lagerstroemia indica	95.27
IBA treatment of Phyllostachys Pubescens	92.47
		IBA treatment of Echinacea purpurea	95.23
IBA treatment of Lagerstroemia indica flower	93.45

Example 2

A rooting agent containing garden negundo chastetree seeds is prepared by the following steps:

s1, weighing 50 parts of garden negundo chastetree seed powder, 15 parts of mint root powder, 7 parts of houttuynia cordata powder, 9 parts of couchgrass root powder, 1 part of floral leaf giant reed root powder, 8 parts of dutchmanspipe flower powder, 2 parts of six-edge chrysanthemum powder, 5 parts of Chinese red clubmoss herb powder, 3 parts of beautiful evening primrose extract, 15 parts of yellow meadowrue herb extract, 0.5 part of wheat grass powder, 0.8 part of larch extract, 0.6 part of xanthium sibiricum extract and 1.8 parts of purslane extract according to the mass part ratio of the components;

s2, sterilizing and drying garden vitex negundo seed powder, mint root powder, houttuynia cordata powder, couch grass root powder, floral leaf giant reed root powder and dutchmanspipe flower powder, putting into a reaction kettle with a thermometer, a heating device and a stirring device, stirring for 10min at 45 ℃ at the stirring speed of 250r/min, heating to 50 ℃, adding six-arrier chrysanthemum powder, common clubmoss herb powder, beautiful evening primrose extract and yellow lotus extract, and continuing stirring for 35min at the stirring speed of 220 r/min;

s3, heating to 75 ℃, adding wheat straw powder, larch extracting solution, xanthium sibiricum extracting solution and purslane extracting solution, and stirring for 25min at the stirring speed of 220 r/min;

and S4, stirring completely, putting into a ball mill, ball-milling for 50min, filtering, sterilizing with ozone, and filling.

The raw materials of the embodiment are widely available, the cost is low, the storage period is 1 year, the environment cannot be polluted, the river channel cannot be blocked, and the configuration is simple. The invention takes the horticultural negundo chastetree seeds as the main component, grinds the negundo chastetree seeds into powder, fully cooperates with the rest powder components, has sufficient active nutrient substances, improves the activity of SOD in the cutting seedlings, ensures that the root systems of the cutting seedlings grow well, reduces the morbidity of bacterial leaf rot, leaf spot and ear root rot, ensures that the morbidity of root rot, root tumor and rust disease is 0 percent, and simultaneously can improve the cold resistance, the reproduction rate, the survival rate and the rooting rate of the cutting seedlings and the quality of the cutting seedlings and form callus.

The test is carried out in No. 6 greenhouse of new No. 15 test field of Nanjing perennial root botanical garden (east longitude 118 degrees 21 '-118 degrees 46', north latitude 30 degrees 51 '-32 degrees 15'), the greenhouse is covered with 80-90% of sunshade net, the temperature in the greenhouse is 25 + -2 ℃, and the humidity is 80-90%. The land belongs to subtropical monsoon climate, has large difference between the annual seasons, obvious abundance and darkness, uneven distribution of rainfall, annual average temperature of 15.4 ℃, 39.7 ℃ and extremely low temperature of-13.1 ℃.

Branches of the mother plants of the perennial carnation, the purple coneflower and the lagerstroemia indica which grow vigorously, develop fully and have no diseases and insect pests are collected at 7 am to be used as cutting slips. And spraying water on the collected spike strips by using a spray-shaped spraying pot, and then bringing the spike strips back to the greenhouse for treatment. Cutting the ear strips into 7-10cm long, reserving 2 leaves at the top, cutting off the leaves 1/2-2/3, cutting the upper end horizontally, and cutting the lower end obliquely at a position 0.5cm below the knot. The cut ear strips are divided into 12 groups according to 36 groups. The rooting agent was the product of example 1 and IBA. When the scion is treated, the scion which is cut is respectively soaked in a rooting agent for 10min, and then the scion is naturally dried in the shade. Inserting the treated scion wood into peat soil sterilized by 1000 times of carbendazim: the volume ratio of perlite is 1: 3, after the cutting, watering thoroughly to ensure that the cutting slips are fully contacted with the matrix. Finally, pouring the mixture once by 1000 times of carbendazim.

Watering is carried out according to the temperature and humidity in the greenhouse and the moisture condition of the matrix, so that the temperature in the greenhouse is kept at 20-25 ℃, the humidity is kept above 80%, the watering amount in the first week is large, and the watering amount is reduced when the spike sprouts, so as to ensure the normal development of the root system. Pouring 1000 times of carbendazim every other week, frequently observing the condition and timely removing withered and fallen leaves.

Example 1 and IBA had different effects on the rooting of carnation, echinacea and lagerstroemia indica cuttings. As can be seen from the table 2, the cuttage rooting rate of the ratoon carnation, the echinacea purpurea and the lagerstroemia indica flower can be effectively improved by the embodiment 1 and the IBA, and the product can replace chemical reagents and is more environment-friendly.

TABLE 2 Effect of hormones on cuttage rooting of Phyllostachys perennialis, Echinacea purpurea and Lagerstroemia indica

Treatment of	Average rooting percentage/%)
		Example 1 treatment of Phyllostachys perennis	92.58
EXAMPLE 1 treatment of Echinacea purpurea	93.63
		Example 1 treatment of Lagerstroemia indica	94.32
IBA treatment of Phyllostachys Pubescens	91.26
		IBA treatment of Echinacea purpurea	94.37
IBA treatment of Lagerstroemia indica flower	93.58

Example 3

A rooting agent containing garden negundo chastetree seeds is prepared by the following steps:

s1, weighing 40 parts of garden negundo chastetree seed powder, 20 parts of mint root powder, 6 parts of houttuynia cordata powder, 6 parts of couch grass root powder, 5 parts of bamboo leaf and reed root powder, 6 parts of dutchmanspipe flower powder, 5 parts of six-edge chrysanthemum powder, 4 parts of Chinese red clubmoss herb powder, 5 parts of beautiful evening primrose extract, 10 parts of yellow meadowrue herb extract, 1 part of wheat grass powder, 0.5 part of larch extract, 0.7 part of siberian cocklebur extract and 1.5 parts of purslane extract according to the mass part ratio of the components;

s2, sterilizing and drying garden vitex negundo seed powder, mint root powder, houttuynia cordata powder, couch grass root powder, floral leaf giant reed root powder and dutchmanspipe flower powder, putting into a reaction kettle with a thermometer, a heating device and a stirring device, stirring for 20min at 40 ℃, stirring at the speed of 230r/min, heating to 55 ℃, adding six-arris chrysanthemum powder, common clubmoss herb powder, beautiful evening primrose extract and yellow lotus extract, and continuing stirring for 25min at the stirring speed of 225 r/min;

s3, heating to 70 ℃, adding wheat straw powder, larch extracting solution, xanthium sibiricum extracting solution and purslane extracting solution, and stirring for 35min at the stirring speed of 215 r/min;

and S4, stirring completely, putting into a ball mill, ball-milling for 40min, filtering, sterilizing with ozone, and filling.

The raw materials of the embodiment are wide in source, the cost is low, the storage period is 1.5 years, the environment cannot be polluted, the river channel cannot be blocked, and the configuration is simple. The invention takes the horticultural negundo chastetree seeds as the main component, grinds the negundo chastetree seeds into powder, fully cooperates with the rest powder components, has sufficient active nutrient substances, improves the activity of SOD in the cutting seedlings, ensures that the root systems of the cutting seedlings grow well, reduces the morbidity of bacterial leaf rot, leaf spot and ear root rot, ensures that the morbidity of root rot, root tumor and rust disease is 0 percent, and simultaneously can improve the cold resistance, the reproduction rate, the survival rate and the rooting rate of the cutting seedlings and the quality of the cutting seedlings and form callus.

The test is carried out in No. 6 greenhouse of new No. 15 test field of Nanjing perennial root botanical garden (east longitude 118 degrees 21 '-118 degrees 46', north latitude 30 degrees 51 '-32 degrees 15'), the greenhouse is covered with 80-90% of sunshade net, the temperature in the greenhouse is 25 + -2 ℃, and the humidity is 80-90%. The land belongs to subtropical monsoon climate, has large difference between the annual seasons, obvious abundance and darkness, uneven distribution of rainfall, annual average temperature of 15.4 ℃, 39.7 ℃ and extremely low temperature of-13.1 ℃.

Branches of the mother plants of the perennial carnation, the purple coneflower and the lagerstroemia indica which grow vigorously, develop fully and have no diseases and insect pests are collected at 7 am to be used as cutting slips. And spraying water on the collected spike strips by using a spray-shaped spraying pot, and then bringing the spike strips back to the greenhouse for treatment. Cutting the ear strips into 7-10cm long, reserving 2 leaves at the top, cutting off the leaves 1/2-2/3, cutting the upper end horizontally, and cutting the lower end obliquely at a position 0.5cm below the knot. The cut ear strips are divided into 12 groups according to 36 groups. The rooting agent was the product of example 1 and IBA. When the scion is treated, the scion which is cut is respectively soaked in a rooting agent for 10min, and then the scion is naturally dried in the shade. Inserting the treated scion wood into peat soil sterilized by 1000 times of carbendazim: the volume ratio of perlite is 1: 3, after the cutting, watering thoroughly to ensure that the cutting slips are fully contacted with the matrix. Finally, pouring the mixture once by 1000 times of carbendazim.

Watering is carried out according to the temperature and humidity in the greenhouse and the moisture condition of the matrix, so that the temperature in the greenhouse is kept at 20-25 ℃, the humidity is kept above 80%, the watering amount in the first week is large, and the watering amount is reduced when the spike sprouts, so as to ensure the normal development of the root system. Pouring 1000 times of carbendazim every other week, frequently observing the condition and timely removing withered and fallen leaves.

Example 1 and IBA had different effects on the rooting of carnation, echinacea and lagerstroemia indica cuttings. As can be seen from Table 3, both the example 1 and IBA can effectively improve the cuttage rooting rate of the ratoon carnation, the echinacea purpurea and the lagerstroemia indica flower, and the product can replace chemical reagents and is more environment-friendly.

TABLE 3 Effect of hormones on cuttage rooting of Phyllostachys perennialis, Echinacea purpurea and Lagerstroemia indica

Treatment of	Average rooting percentage/%)
		Example 1 treatment of Phyllostachys perennis	94.16
EXAMPLE 1 treatment of Echinacea purpurea	93.28
		Example 1 treatment of Lagerstroemia indica	95.34
IBA treatment of Phyllostachys Pubescens	93.87
		IBA treatment of Echinacea purpurea	94.29
IBA treatment of Lagerstroemia indica flower	93.18

As noted above, while the present invention has been shown and described with reference to certain preferred embodiments, it is not to be construed as limited thereto. Various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.