阅读说明：本技术 一种驱蚊组合物及含有该组合物的花露水 (Mosquito repellent composition and toilet water containing same ) 是由 范松洪 刘慧敏 刘桂林 刘洪智 刘清浩 刘鸿钊 于 2021-07-20 设计创作，主要内容包括：本发明公开了一种驱蚊组合物及含有该组合物的花露水；所述驱蚊组合物含有如下重量份的组分：薰衣草提取物10-15份,菊花提取物8-12份,南非钩麻根提取物6-10份,甘草根提取物5-10份,金银花提取物4-8份,灯油藤提取物4-8份；属于日化用品领域。含有该组合物的花露水具有驱蚊、止痒、抗菌、消炎消肿的作用。(The invention discloses a mosquito repellent composition and floral water containing the composition; the mosquito repellent composition comprises the following components in parts by weight: 10-15 parts of lavender extract, 8-12 parts of chrysanthemum extract, 6-10 parts of devil's-tongue root extract, 5-10 parts of licorice root extract, 4-8 parts of honeysuckle extract and 4-8 parts of common alstonia extract; belongs to the field of daily chemical articles. The floral water containing the composition has mosquito repellent, itching relieving, antibacterial, antiinflammatory and repercussive effects.)

1. The mosquito repellent composition is characterized by comprising the following components in parts by weight: 10-15 parts of lavender extract, 8-12 parts of chrysanthemum extract, 6-10 parts of devil's-tongue root extract, 5-10 parts of licorice root extract, 4-8 parts of honeysuckle extract and 4-8 parts of common alstonia root extract.

2. The mosquito repellent composition according to claim 1, further comprising 4-7 parts of vetiver extract.

3. The mosquito repellent composition according to claim 2, further comprising 3-6 parts of kochia scoparia extract.

4. A mosquito repellent composition according to claim 3, further comprising 3-6 parts of geranium extract.

5. A mosquito repellent composition according to claim 1, wherein the preparation method of the marsdenia tenacissima extract in the composition is as follows:

s1: screening leaves, flowers and seeds of the common alstonia herbs, soaking the leaves, flowers and seeds in honey for 8-16h after ultraviolet disinfection, and then airing, wherein the mass ratio of the common alstonia herbs to the honey is 1: (3-6);

s2: placing the aired calamus margaritae in the step S1 into a crusher with a built-in 80-120-mesh screen for crushing, and then adding a soaking solution, wherein the volume weight ratio of the soaking solution to the powder is (4-6) L: 1kg, and carrying out ultrasonic treatment for 10-20 minutes to obtain a suspension;

s3: spraying the suspension obtained in the step S2 into mist-shaped liquid drops by using an atomizer, and simultaneously carrying out countercurrent extraction by using an extraction solvent to obtain a primary extract;

s4: filtering the primary extract in S3, concentrating the obtained filtrate under reduced pressure at-0.08-0.1 Mpa and 40-50 deg.C to 1/3-1/6 of the volume of the original extract, and centrifuging to obtain the extract of calyx Seu fructus Physalis.

6. A mosquito repellent composition according to claim 5, wherein the soaking solution in the step S2 and the extraction solvent in the step S3 are all ethanol water solutions with volume ratio concentration of 70-90%.

7. A toilet water comprising the mosquito repellent composition according to any one of claims 1 to 4.

8. The floral water as claimed in claim 7, which is prepared from the following components in parts by mass: 40-60 parts of alcohol, 0.05-0.2 part of menthol, 0.05-0.2 part of borneol, 0.2-1.3 parts of essence, 3-10 parts of mosquito repellent composition and 29-56 parts of deionized water.

Technical Field

The invention relates to a composition, in particular to a mosquito repellent composition, and also relates to floral water containing the composition, belonging to the field of daily chemical products.

Background

Summer is a season in which mosquitoes and insects propagate fast and bacteria grow, after human skin is bitten by mosquitoes or infected by bacteria, pruritus, red swelling, inflammation and the like are easily caused, and even dengue fever and other diseases are caused, night sleep is affected, people are distracted, depressed and anxious, and the influence on the physical and mental health of the human body is great.

The toilet water is a daily chemical product which is frequently used in daily life, and has the functions of repelling mosquitoes, sterilizing and relieving itching. The common floral water is prepared by stirring a large amount of alcohol, water, essence and certain specific chemical reagents, and achieves the effects of relieving itching and repelling mosquitoes. The mosquito repellent active ingredients added in most toilet water in the current market are chemical ingredients such as anophelifuge, deet, diethyltoluketone, deet and the like, and the long-term use of the mosquito repellent active ingredients can cause damage to digestive tracts, respiratory tracts and the like, induce diseases such as asthma and even cause toxic and side effects such as carcinogenesis, teratogenesis and the like. In addition, menthol can be added, borneol has the effects of transient cooling and relieving, and salicylic acid compounds have the effects of sterilizing and diminishing inflammation, but the irritation is large, and skin allergy is easily caused.

Therefore, there is a need to develop a safe and effective floral water with mosquito repelling effect.

Disclosure of Invention

In view of the above problems, it is an object of the present invention to provide a mosquito repellent composition, which can prevent mosquito bites and has the effects of relieving itching, resisting bacteria, diminishing inflammation and diminishing swelling.

The invention also aims to provide toilet water containing the mosquito repellent composition.

In order to achieve the above object, the first technical solution provided by the present invention is as follows:

the mosquito repellent composition comprises the following components in parts by weight: 10-15 parts of lavender extract, 8-12 parts of chrysanthemum extract, 6-10 parts of devil's-tongue root extract, 5-10 parts of licorice root extract, 4-8 parts of honeysuckle extract and 4-8 parts of common alstonia root extract.

Furthermore, the mosquito repellent composition also comprises 4-7 parts of vetiver extract.

Furthermore, the mosquito repellent composition also comprises 3-6 parts of a kochia scoparia extract.

Furthermore, the mosquito repellent composition also comprises 3-6 parts of geranium extract.

Furthermore, the preparation method of the calamus margaritae extract in the mosquito repellent composition comprises the following steps:

s1: screening leaves, flowers and seeds of the common alstonia herbs, soaking the leaves, flowers and seeds in honey for 8-16h after ultraviolet disinfection, and then airing, wherein the mass ratio of the common alstonia herbs to the honey is 1: (3-6);

s2: placing the aired calamus margaritae in the step S1 into a crusher with a built-in 80-120-mesh screen for crushing, and then adding a soaking solution, wherein the volume weight ratio of the soaking solution to the powder is (4-6) L: 1kg, and carrying out ultrasonic treatment for 10-20 minutes to obtain a suspension;

s3: spraying the suspension obtained in the step S2 into mist-shaped liquid drops by using an atomizer, and simultaneously carrying out countercurrent extraction by using an extraction solvent to obtain a primary extract;

s4: filtering the primary extract in S3, concentrating the obtained filtrate under reduced pressure at-0.08-0.1 Mpa and 40-50 deg.C to 1/3-1/6 of the volume of the original extract, and centrifuging to obtain the extract of calyx Seu fructus Physalis.

Further, in the preparation method of the peruvian groundcherry herb extract in the mosquito repellent composition, the soaking solution in the step S2 is extracted by using ethanol water solution with the volume ratio concentration of 70-90% in the step S3.

The second technical scheme provided by the invention is as follows:

a toilet water contains the mosquito repellent composition.

Further, the floral water comprises the following components in parts by mass: 40-60 parts of alcohol, 0.05-0.2 part of menthol, 0.05-0.2 part of borneol, 0.2-1.3 parts of essence, 3-10 parts of mosquito repellent composition and 29-56 parts of deionized water.

Compared with the prior art, the technical scheme provided by the invention has the following beneficial effects:

1. the mosquito repellent composition takes the natural plant extract as an active ingredient, has the effects of repelling mosquitoes, relieving itching and inhibiting bacteria, and is safe and effective. The chrysanthemum extract contains pyrethrin, has a good mosquito repelling effect, also has the effects of clearing away heat and toxic materials, inhibiting bacteria and the like, and can enhance the mosquito repelling effect by mixing the two components in a certain proportion; the radix Harpagophyti extract has astringent, antipruritic, antiinflammatory, and antibacterial effects, the radix Glycyrrhizae extract has antiinflammatory and antipruritic effects, the flos Lonicerae has antibacterial and antiinflammatory effects, and the caulis et folium Periplocae Forrestii extract has effects of dredging channels, relieving pain, promoting blood circulation, removing toxic substance, and treating snake and insect bite. The extracts are compounded, so that a good mosquito repelling effect can be achieved, the symptoms of skin itching, red swelling and pain after mosquito bites can be effectively relieved, and the mosquito repellent is safe to a human body and has no side effect after long-term use.

2. The vetiver extract has the functions of repelling mosquitoes and flies, diminishing inflammation, sterilizing, relieving itching and resisting bacteria, the kochia fruit extract can clear heat, diminish inflammation, detoxify and relieve itching, and the geranium extract can activate blood circulation, remove meridian obstruction, clear heat and detoxify and also has the antibacterial function. According to the technical scheme, the vetiver extract, the kochia scoparia extract and the geranium extract are compounded, so that the effects of mosquito repelling, inflammation diminishing, swelling diminishing, itching relieving and bacteria resisting of the mosquito repelling composition can be further enhanced.

3. The common rush herb extract in the composition is prepared by a special method, and is soaked by honey, so that the dissolution of active ingredients in geranium is enhanced, and the honey has the antibacterial and anti-inflammatory effects and can enhance the efficacy of the common rush herb extract. The ultrasonic treatment and atomization extraction technology are combined, so that the extraction efficiency is greatly promoted, the usage amount of the solvent is reduced, and the dissolution rate of the active ingredients is increased.

4. The toilet water is added with the mosquito repellent composition, and a small amount of menthol and borneol are added, so that the toilet water has the effects of refreshing brain, repelling mosquitoes, relieving itching, diminishing inflammation, diminishing swelling and resisting bacteria, and is safe and effective.

Detailed Description

The technical solution of the present invention will be described in further detail with reference to the following embodiments, but the present invention is not limited thereto. Those skilled in the art to which the invention pertains will readily appreciate that modifications and alterations may be made without departing from the spirit and scope of the invention, which is defined by the appended claims. In addition, the raw materials adopted in the technical scheme provided by the invention are prepared by conventional means or purchased through commercial channels except for special instructions.

The lavender extract is obtained from Guangzhou Nuoran biological technology, the chrysanthemum extract is obtained from Yanghuai chemical engineering technology, Inc., the south Africa radix Rapae extract is obtained from Guangzhou Bochi biological technology, Inc., the licorice root extract and the Kochia scoparia extract are obtained from Yuanzhou technology development, Inc., Chongqing, the honeysuckle extract is obtained from Shanghai Zhen trade, the vetiver extract is obtained from Hangzhou Bofei import and export, Inc., and the geranium extract is obtained from Beijing Lingbao technology, Inc.

Example 1

A mosquito repellent composition comprises the following components in parts by weight: 15 parts of lavender extract, 10 parts of chrysanthemum extract, 10 parts of south African devil's claw root extract, 7 parts of licorice root extract, 4 parts of honeysuckle extract and 6 parts of common alstonia root extract.

Example 2

A mosquito repellent composition comprises the following components in parts by weight: 11 parts of lavender extract, 12 parts of chrysanthemum extract, 8 parts of south African devil's claw root extract, 5 parts of licorice root extract, 6 parts of honeysuckle extract and 8 parts of common alstonia root extract.

Example 3

A mosquito repellent composition comprises the following components in parts by weight: 13 parts of lavender extract, 11 parts of chrysanthemum extract, 6 parts of southern African devil's claw root extract, 10 parts of licorice root extract, 7 parts of honeysuckle extract and 4 parts of common alstonia root extract.

Example 4

A mosquito repellent composition comprises the following components in parts by weight: 10 parts of lavender extract, 8 parts of chrysanthemum extract, 7 parts of south African devil's claw root extract, 10 parts of licorice root extract, 5 parts of honeysuckle extract and 7 parts of common alstonia root extract.

Example 5

A mosquito repellent composition comprises the following components in parts by weight: 11 parts of lavender extract, 12 parts of chrysanthemum extract, 8 parts of devil's-tongue root extract, 5 parts of licorice root extract, 6 parts of honeysuckle extract, 8 parts of common alstonia root extract and 6 parts of vetiver extract.

Example 6

A mosquito repellent composition comprises the following components in parts by weight: 14 parts of lavender extract, 10 parts of chrysanthemum extract, 9 parts of radix harpagophyti extract, 7 parts of licorice root extract, 8 parts of honeysuckle extract, 5 parts of common alstonia root extract, 4 parts of vetiver extract and 6 parts of kochia fruit extract.

Example 7

A mosquito repellent composition comprises the following components in parts by weight: 12 parts of lavender extract, 9 parts of chrysanthemum extract, 10 parts of devil's-tongue root extract, 9 parts of licorice root extract, 4 parts of honeysuckle extract, 6 parts of common alstonia root extract, 7 parts of vetiver extract, 3 parts of kochia fruit extract and 6 parts of geranium extract.

Example 8

A mosquito repellent composition comprises the following components in parts by weight: 11 parts of lavender extract, 12 parts of chrysanthemum extract, 8 parts of devil's-tongue root extract, 5 parts of licorice root extract, 6 parts of honeysuckle extract, 8 parts of common alstonia root extract, 6 parts of vetiver extract, 5 parts of kochia extract and 3 parts of geranium extract.

In the mosquito repellent compositions of examples 1-8, the preparation method of the marsdenia tenacissima extract is as follows:

s1: screening leaves, flowers and seeds of the common alstonia herbs, performing ultraviolet disinfection, soaking in honey for 8-16h (preferably 12h), and then airing, wherein the mass ratio of the common alstonia herbs to the honey is 1: (3-6) (preferably 1: 5);

s2: placing the aired calamus margaritae in the step S1 into a crusher with a screen of 80-120 meshes (preferably 100 meshes) for crushing, and then adding a soaking solution, wherein the volume weight ratio of the soaking solution to the powder is (4-6) L: 1kg (preferably 5L: 1kg), sonicating for 10-20 minutes (preferably 15 minutes) to obtain a suspension;

s3: spraying the suspension obtained in the step S2 into mist-shaped liquid drops by using an atomizer, and simultaneously carrying out countercurrent extraction by using an extraction solvent to obtain a primary extract;

s4: filtering the primary extract in S3, concentrating the filtrate under reduced pressure at-0.08-0.1 Mpa and 40-50 deg.C (preferably 45 deg.C) to 1/3-1/6 (preferably 1/4) of the volume of the primary extract, and centrifuging to obtain the extract.

Example 9

The floral water comprises the following components in parts by mass: 40 parts of alcohol, 0.2 part of menthol, 0.05 part of borneol, 1.3 parts of essence, 10 parts of the mosquito repellent composition in example 1 and 55.45 parts of deionized water.

Example 10

The floral water comprises the following components in parts by mass: 50 parts of alcohol, 0.1 part of menthol, 0.1 part of borneol, 0.8 part of essence, 6 parts of the mosquito repellent composition in example 2 and 43 parts of deionized water.

Example 11

The floral water comprises the following components in parts by mass: 50 parts of alcohol, 0.1 part of menthol, 0.1 part of borneol, 0.8 part of essence, 6 parts of the mosquito repellent composition in example 5 and 43 parts of deionized water.

Example 12

The floral water comprises the following components in parts by mass: 60 parts of alcohol, 0.05 part of menthol, 0.2 part of borneol, 0.2 part of essence, 3 parts of the mosquito repellent composition in example 6 and 29.55 parts of deionized water.

Example 13

The floral water comprises the following components in parts by mass: 50 parts of alcohol, 0.1 part of menthol, 0.1 part of borneol, 0.8 part of essence, 6 parts of the mosquito repellent composition in example 8 and 43 parts of deionized water.

The floral water of examples 9-13 was prepared by: adding alcohol into a stirring pot, starting stirring, sequentially adding essence, menthol and borneol, stirring for dissolving completely, then adding deionized water and the mosquito repellent composition, stirring uniformly, filtering, inspecting, and discharging after the product is qualified.

In order to better illustrate the beneficial effects of the technical solutions provided by the present application, some comparative examples of the present application in the development process are given below.

Comparative example 1

A mosquito repellent composition is prepared by replacing Lavender extract with flos Chrysanthemi extract in the same amount as in example 2, and the other components and contents are the same.

Comparative example 2

A mosquito repellent composition comprises Glycyrrhrizae radix extract as equivalent as radix Harpagophyti Latifoliae extract in example 2, and other components and contents are the same.

Comparative example 3

A mosquito repellent composition is prepared by replacing caulis et folium Hedyotis Hedyotideae extract with flos Lonicerae extract, and the other components and contents are the same as in example 2.

Comparative example 4

A mosquito repellent composition comprises the same components and content as those of the mosquito repellent composition in example 2, except that the preparation method of the calamus margaritae extract omits the step of S3, and the suspension obtained in the step S2 is directly filtered and then concentrated under reduced pressure.

Comparative example 5

A mosquito repellent composition comprises the following components in parts by weight: 8 parts of lavender extract, 15 parts of chrysanthemum extract, 9 parts of south African devil's claw root extract, 5 parts of licorice root extract, 2 parts of honeysuckle extract and 12 parts of common alstonia root extract.

Control sample

The mosquito repellent composition of example 2 contained in example 10 was replaced with the mosquito repellent composition of comparative examples 1 to 5 in equal amounts, and the other components and the contents thereof were not changed, corresponding to toilet water of comparative examples 1 to 5, and the preparation method thereof was the same as that of example 10.

The efficacy proves and tests:

1. patch test

To detect the irritation of the floral water, a skin patch test was performed.

The test method comprises the following steps: 30 healthy volunteers were selected, and 48-hour closed class test of the toilet water of examples 9-13 of the present invention on the back skin of 30 volunteers showed no irritation, which indicates that the mosquito repellent composition of the present invention is safe and the toilet water containing the composition of the present invention is also safe and non-irritant.

2, mosquito repelling Effect test

Test samples: inventive examples 9-13, comparative examples 1-5.

Testing mosquitoes: standard test insects of sensitive strains raised in a laboratory are adopted. Aedes albopictus, female adult insects which do not suck blood 3-5 days after emergence.

The test method comprises the following steps: the test method is carried out according to GB/T13917.9-2009 (pesticide registration sanitary insecticide indoor efficacy test and evaluation part 9: repellent), wherein 6 volunteers with qualified offensive power are selected for each group of the repellent test and are tested, and the test is carried out on each half of men and women. The effective protection time (h) of the repellent was recorded and averaged, and the experimental results are shown in table 1.

TABLE 1 mosquito repelling effect test results

Sample (I)	Effective guard time/h
		Example 9	8.8
Example 10	9.2
		Example 11	10.6
Example 12	10.4
		Example 13	11.5
Comparative sample 1	6.5
		Comparative sample 2	7.3
Comparative sample 3	7.6
		Comparative sample 4	7.0
Comparative sample 5	7.9

As can be seen from the table 1, the toilet water containing the mosquito repellent composition has a remarkable mosquito repellent effect, and the effective protection time of the toilet water on volunteers is more than 8 hours. Compared with the embodiment 10 of the invention, the content of the chrysanthemum extract is increased in the floral water of the comparison sample 1 without adding the lavender extract, the liquorice root extract is used for replacing the devil's-claw root extract in the floral water of the comparison sample 2 in an equivalent manner, the honeysuckle extract is used for replacing the derris japonica extract in the floral water of the comparison sample 3 in an equivalent manner, the effective protection time of the comparison samples 1-3 on volunteers is obviously reduced, the mosquito repellent effect is not the embodiment 10 of the invention, and the synergistic effect of all the components of the mosquito repellent composition in the floral water of the invention is proved to have the obvious mosquito repellent effect. The floral water prepared in the embodiment 13 of the invention has the most remarkable mosquito repellent effect. Comparative example 4 a preparation method of the extract of derris elliptica in floral water was different from the preparation method in example 10, and the mosquito repellent effect was reduced as compared with example 10. The content of the four components in the 5-sample floral water is adjusted, and the mosquito repelling effect is lower than that in the example 10, which shows that the mixture ratio of the lavender extract, the chrysanthemum extract, the honeysuckle extract and the common rush extract in a proper range can improve the mosquito repelling effect of the composition.

3, anti-inflammatory and detumescence test

Test samples: : inventive examples 9-13, comparative examples 1-5.

The test method comprises the following steps: 110 mice were divided into 11 groups of 10 mice each, each half of which was male and female. The blank control group was given 10ml of distilled water, and the other groups were given equal amounts of the floral water of examples 9-13, comparative examples 1-5, respectively, 1 time a day for 3 consecutive days. 1h after the last administration, the right ear of the mouse was smeared with 0.05ml of xylene, and the left ear was used as a control. After 1h, the animals were sacrificed, and the same part of both ears was removed with a punch having a diameter of 6cm, weighed, and the mass was recorded to calculate the swelling rate (left ear weight-right ear weight/right ear weight) ×%, and the results are shown in table 2.

TABLE 2 anti-inflammatory and repercussive test results

Sample (I)	Swelling degree/g
		Example 9	0.19±0.13
Example 10	0.18±0.15
		Example 11	0.15±0.08
Example 12	0.11±0.10
		Example 13	0.07±0.05
Comparative sample 1	0.37±0.16
		Comparative sample 2	0.43±0.18
Comparative sample 3	0.39±0.10
		Comparative sample 4	0.48±0.19
Comparative sample 5	0.34±0.21
		Blank control group	0.68±0.25

As can be seen from the results of Table 3, the swelling degree results of examples 9-13 were all significantly lower than those of the blank control group, indicating that the floral water of the present invention was able to significantly reduce the swelling degree of the mice. Comparison of the results of comparative examples 1-3 with those of example 10 shows that omission of any one of lavender extract, devil's claw root extract, and common alstonia extract reduces the swelling-diminishing effect. Comparison of comparative example 4 with the results of example 10 shows that the preparation method of the extract of common alstonia root of the present application can better increase the content of the active ingredient. The content of lavender extract, chrysanthemum extract, honeysuckle extract and common alstonia root extract is adjusted in the comparison sample 5, and the result shows that the anti-inflammation and detumescence effect of the floral water is remarkably lower than that of the floral water in the example 10, which indicates that the proportion in a proper range can also influence the anti-inflammation and detumescence effect of the floral water.

4, bacteriostatic test

Test samples: inventive examples 9-13, comparative examples 1-5.

The test method comprises the following steps: preparing a test sample into a solution to be tested for later use, heating the sterilized solid culture medium to be molten, and pouring the molten solid culture medium into sterile culture dishes when the temperature of the culture medium is cooled to 50 ℃, wherein the amount of the culture medium in each culture dish is 25 ml. After the plate is naturally dried, 0.1ml of the solution to be tested and 0.1ml of the test bacterial suspension (escherichia coli, staphylococcus aureus, streptococcus thermophilus and lactobacillus) are respectively transferred and evenly smeared on the dosing plate, and the test solution is replaced by the same amount of sterile water in the control group. Culturing at 37 ℃ for 24h, counting the number of colonies, and calculating the bacteriostasis rate, wherein the bacteriostasis rate is (the number of control colonies-the number of treated colonies)/the number of control colonies multiplied by 100%. The results are shown in Table 3.

TABLE 3 results of the bacteriostatic test

Sample (I)	Escherichia coli	Staphylococcus aureus	Streptococcus thermophilus	Lactobacillus strain
					Example 9	85.5％	90.3％	86.7％	88.0％
Example 10	86.8％	91.8％	88.0％	89.1％
					Example 11	88.4％	92.6％	90.4％	90.7％
Example 12	91.3％	94.2％	91.5％	92.8％
					Example 13	93.0％	96.5％	92.6％	94.6％
Comparative sample 1	77.3％	83.3％	78.1％	80.3％
					Comparative sample 2	72.7％	73.9％	71.9％	73.4％
Comparative sample 3	75.5％	81.7％	77.0％	78.1％
					Comparative sample 4	70.6％	71.2％	69.2％	71.8％
Comparative sample 5	78.6％	84.0％	79.3％	81.5％

As is apparent from Table 3, the floral water of examples 9 to 13 of the present invention exhibited excellent bacteriostatic effects against Escherichia coli, Staphylococcus aureus, Streptococcus thermophilus and Lactobacillus, and the floral water of example 13 exhibited the best bacteriostatic effect. The bacteriostatic effect of the comparative samples 1-5 is lower than that of the example 10, which shows that the bacteriostatic effect can be remarkably improved by matching and synergizing various components of the mosquito repellent composition in a proper range.

5. Human body test

Test samples: inventive examples 9-13, comparative examples 1-5.

Test subjects: 150 volunteers 15-55 years old.

The test method comprises the following steps: the test subjects were randomly divided into 10 groups of 15 each, and the floral water of the present invention 9 to 13 and comparative examples 1 to 5 were used. The mosquito repellent is used once in the morning and at night every day, is sprayed on the skin or clothes with the exposed hands, legs and the like, is continuously used for one week, and scores the mosquito repellent effect, the detumescence antipruritic effect, the nonirritant effect and the cool feeling. The highest score was 5 parts, and the effect was not good at a score of 3 or less. The average was taken and the results are shown in Table 4.

TABLE 4 human trial Effect Scoring

Sample (I)	Mosquito repelling effect	Detumescence and itching relieving effect	Has no irritation	Feeling of coolness
					Example 9	4.5	4.4	4.8	4.8
Example 10	4.6	4.5	4.7	4.9
					Example 11	4.7	4.7	4.6	4.9
Example 12	4.7	4.5	4.8	4.8
					Example 13	4.9	4.8	4.9	4.9
Comparative sample 1	3.7	3.9	4.4	4.7
					Comparative sample 2	3.7	3.8	4.2	4.8
Comparative sample 3	3.8	3.5	4.2	4.7
					Comparative sample 4	3.6	3.7	4.3	4.8
Comparative sample 5	3.9	3.9	4.6	4.8

As can be seen from the results in table 4, the toilet waters of examples 9 to 11 containing the mosquito repellent composition of the present invention have excellent mosquito repellent, detumescence and itching relieving effects, and the toilet water of the present invention has no irritation to the skin and also has a refreshing feeling after use. The mosquito repellent effect and the detumescence antipruritic effect of the comparison samples 1-5 are not the same as those of the toilet water in the examples 9-11, which shows that the mosquito repellent composition has the synergistic effect of the components to achieve good mosquito repellent, detumescence antipruritic effect and improve the use comfort. The best using effect is obtained by combining various using results, and the using effect of the mosquito-repellent composition is shown in the embodiment 13 of the invention, so that the mosquito-repellent, swelling-reducing and itching-relieving effects are best when the composition is prepared from a plurality of components including lavender extract, chrysanthemum extract, devil's claw root extract, licorice root extract, honeysuckle extract, common alstonia root extract, vetiver extract, kochia fruit extract and geranium extract.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, simplifications and equivalents which do not depart from the spirit and principle of the present invention are intended to be included within the scope of the present invention.