阅读说明：本技术 一种具有皮肤和毛发养护功效的犬用植物组合物及其应用 (Dog plant composition with skin and hair care effect and application thereof ) 是由 黄伟 于 2021-07-21 设计创作，主要内容包括：本发明涉及一种具有皮肤和毛发养护功效的犬用植物组合物及其应用,所述具有皮肤和毛发养护功效的犬用植物组合物包括青葙子提取物、鼠尾草提取物、马鞭草提取物、迷迭香提取物、金缕梅提取物和金盏花提取物,六种成分相辅相成,协同增效,对犬具有显著的皮肤养护和毛发养护功效。具体地,该植物组合物能够有效地去除犬体表的螨虫、抑制犬体表的细菌,显著改善犬的皮肤微环境,减少犬的皮肤病的发生；还能够有效平衡犬的皮肤油脂分泌,减少脂溢性皮炎的发生；还能够改善犬毛发干涩不顺滑的现象。(The invention relates to a dog plant composition with skin and hair care effects and application thereof, wherein the dog plant composition with skin and hair care effects comprises a feather cockscomb seed extract, a salvia officinalis extract, a verbena extract, a rosemary extract, a witch hazel extract and a calendula extract, and the six ingredients supplement each other and are synergistic, so that the dog plant composition has remarkable skin care and hair care effects. Specifically, the plant composition can effectively remove mites on the body surface of a dog, inhibit bacteria on the body surface of the dog, remarkably improve the skin microenvironment of the dog and reduce the occurrence of skin diseases of the dog; the skin oil secretion of the dog can be effectively balanced, and the occurrence of seborrheic dermatitis is reduced; can also improve the phenomenon of dry and unsmooth hair of dogs.)

1. The dog plant composition with the skin and hair care effect is characterized by comprising a feather cockscomb seed extract, a sage extract, a verbena extract, a rosemary extract, a witch hazel extract and a calendula extract.

2. The canine plant composition for skin and hair care according to claim 1, wherein the canine plant composition for skin and hair care comprises, by weight, 5-50 parts of feather cockscomb seed extract, 5-50 parts of sage extract, 5-10 parts of verbena extract, 10-40 parts of rosemary extract, 5-10 parts of witch hazel extract, and 20-30 parts of calendula extract.

3. The canine plant composition having skin and hair care effects according to claim 1 or 2, wherein the feather cockscomb seed extract, salvia extract or verbena extract are independently prepared by a method comprising:

pulverizing semen Celosiae, herba Salvia officinalis or herba Verbenae, soaking in water, reflux extracting, concentrating the extractive solution, and oven drying;

preferably, the mass ratio of the water to the feather cockscomb seed, the sage or the verbena is (5-15) to 1;

preferably, the soaking temperature is 15-30 ℃, and the soaking time is 15-30 h;

preferably, the reflux extraction is performed for 1-4 times, each time for 50-90 min.

4. The skin and hair care canine plant composition according to claim 1 or 2, wherein said rosemary extract is prepared by a method comprising:

drying and pulverizing herba Rosmarini officinalis, boiling with 65-75% ethanol by volume, reflux extracting to obtain extractive solution, concentrating, and oven drying;

preferably, the reflux leaching times are 1-4 times, and each time is 20-40 min.

5. The plant composition for dogs having skin and hair care effects according to claim 1 or 2, wherein the witch hazel extract is prepared by a method comprising:

drying and pulverizing Hamamelis virginiana, boiling with 55-65% ethanol by volume, reflux extracting to obtain extractive solution, concentrating, and oven drying;

preferably, the reflux leaching times are 1-4 times, and each time is 20-40 min.

6. The plant composition for dogs having skin and hair care effects as claimed in claim 1 or 2, wherein the calendula extract is prepared by a method comprising:

drying and pulverizing herba Sidae Rhombifoliae, boiling with 70-80 vol% ethanol, reflux extracting to obtain extractive solution, concentrating, and oven drying;

preferably, the reflux leaching times are 1-4 times, and each time is 20-40 min.

7. Use of the canine plant composition having skin and hair care efficacy according to any one of claims 1 to 6 for the preparation of a canine rinse care product;

preferably, the canine wash products comprise canine shampoos, canine hair conditioners, canine lotions;

preferably, the weight percentage of the plant composition for dogs with the skin and hair care efficacy in the washing and protecting product for dogs is 0.1-15%.

8. A shampoo for dogs having skin and hair care effects, which is characterized in that the raw materials for preparing the shampoo for dogs having skin and hair care effects comprise the plant composition for dogs having skin and hair care effects of any one of claims 1 to 6, ecological nutrients, a surfactant, a chelating agent, a humectant, a thickener, a pH regulator, a preservative and water.

9. The shampoo for dogs having skin and hair care effects of claim 8 wherein the ecological nutrients comprise any one or a combination of at least two of inulin, α -glucan oligosaccharide, fructo-oligosaccharide, galacto-oligosaccharide, xylo-oligosaccharide, isomalto-oligosaccharide or soy oligosaccharide; preferably a combination of inulin, alpha-glucan oligosaccharides, galactooligosaccharides and soy oligosaccharides;

preferably, the mass ratio of the inulin to the alpha-glucan oligosaccharide to the galacto-oligosaccharide to the soybean oligosaccharide is (2-3) to 1 (1-2);

preferably, the surfactant comprises any one of sodium laureth sulfate, cocamide DEA, cocamide propyl betaine or lauryl glucoside, or a combination of at least two thereof.

10. The shampoo for dogs having skin and hair care effects of claim 8 or 9 wherein the humectant comprises any one or a combination of at least two of glycerin, propylene glycol, 1, 3-butylene glycol, sorbitol, polyethylene glycol, hyaluronic acid, ceramide, or collagen;

preferably, the chelating agent comprises disodium EDTA;

preferably, the thickener comprises sodium chloride;

preferably, the pH adjusting agent comprises citric acid and/or sodium citrate.

Technical Field

The invention belongs to the technical field of pet daily chemical products, relates to a plant composition for dogs and application thereof, and particularly relates to a plant composition for dogs with skin and hair care effects and application thereof in preparation of washing and protecting products for dogs.

Background

The pet hair is long and easily gets dirty, and needs to be cleaned regularly. Some pets feel that dogs are dirty, so dogs are often helped to take a bath, but a plurality of dogs use shampoo only with attention paid to basic cleaning function and lack hair and skin care ingredients, and the phenomenon that the grease protective layer on the skin of the dogs is damaged easily due to frequent bath of the dogs by using the shampoo, so that the dogs are easy to lose hair, tear stains and the like, and even suffer from skin diseases. Seborrheic dermatitis is one of the common skin diseases of dogs and is typically characterized by skin lesions which are dark yellowish-red spots with clear edges, patches or blotchy papules, and are covered with greasy scales or crusts. The dog has a large amount of bacteria and fungi on the body and skin, is easy to suffer from skin diseases, is very easy to be infected with fungi and has tinea capitis in fact a home meal, and particularly in spring, the dog is the most prone period of tinea capitis.

At present, the raising of pets is very common, and care products for the pets, such as bath cream in the aspect of cleaning, pay more and more attention to the comprehensiveness of the quality and the efficacy of the products.

CN111773164A discloses a bath lotion for pets, which takes lightyellow sophora root, chinaberry root, macleaya cordata, dahurian angelica root, sessile stemona root, realgar, cactus, polygonum tinctorium, amur corktree bark, rhubarb and rosemary as medicinal materials, and has reasonable compatibility; the prepared shower gel has strong detergency, is easy to clean and rinse, has no stimulation to skin, and the cleaned skin and hair are smooth, tender, cool, comfortable, elastic and fragrant.

CN107049881A discloses a health shampoo for dogs and cats and a preparation method thereof, which comprises the following components by weight in 1000 ml: 10.0-30.0 g of tea polyphenol, 10.0-30.0 g of fructus cnidii extract, 10.0-30.0 g of clove extracting solution, 50.0-70.0 g of sodium laureth sulfate, 5.0-10.0 g of sodium hyaluronate, 1.0-5.0 g of vitamin E and 1.0-3.0 g of essence, wherein the pH value is adjusted to 5.0-6.0 by using a proper amount of citric acid solution, and the balance is distilled water.

However, the pet washing and caring products disclosed in the prior art have relatively single efficacy, and it is very necessary to develop a mild canine washing and caring product which has the efficacies of cleaning, resisting bacteria, killing insects, resisting inflammation, balancing skin grease and caring hair.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a plant composition for dogs and application thereof, in particular to a plant composition for dogs with skin and hair care effects and application thereof in preparing washing and caring products for dogs.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a canine plant composition having skin and hair care effects, comprising a feather cockscomb seed extract, a sage extract, a verbena extract, a rosemary extract, a witch hazel extract, and a calendula extract.

Wherein the semen Celosiae contains fatty oil, abundant potassium nitrate, and nicotinic acid (Nicoti-nicacid). The seed is used for medicine, and has the effects of clearing liver and improving vision and reducing blood pressure; the whole herb has the effects of clearing heat and promoting diuresis; the tender stem and leaf can be used as vegetable, or feed.

Wherein the herba Salvia officinalis leaf has effects of sterilizing, resisting toxic substance, removing toxic substance, and removing plague and plague, and can be eaten in cold sauce; the stem, leaf and flower can be used as tea for drinking, and has effects of cleaning in vivo oil, promoting circulation, and caring skin. Can also be eaten as seasoning.

The herba Verbenae whole plant is used for medicine, has cool property and slightly bitter taste, and has effects of cooling blood, removing blood stasis, dredging channels, clearing heat, removing toxic substance, relieving itching, expelling intestinal parasites and relieving flatulence.

The rosemary is a rare natural spice plant, gives off a faint scent in the growing season, and has the effects of clearing away the heart-fire and refreshing. Its stem, leaf and flower have pleasant fragrance, and the flower and twig extract aromatic oil. Rosemary has the functions of tranquilizing and restoring consciousness, and has certain curative effect on indigestion and stomachache.

The witch hazel contains various tannins such as Ellagtannin, Hamamelanin and the like, can regulate sebum secretion, has the effects of moisturizing and whitening, has the effects of calming and soothing, has the effect of improving pet skin cracks, and can effectively help the regeneration capability of skin at night. Because of the obvious curative effects of astringing, controlling oil and sterilizing, the skin oil-removing ointment is a poor choice for skin with serious oil-removing condition.

Wherein the flowers and leaves of the calendula have anti-inflammatory and antibacterial effects, and especially have good effects on staphylococcus and streptococcus. The antibacterial component is soluble in alcohol and insoluble in water. In alkaline environment, the effect is better. The flower extract has tranquilizing effect on central nervous system, and can reduce reflex excitability; intravenous injection can cause blood pressure decrease, enhance heart activity, increase heartbeat amplitude, slow down heart rate, promote bile secretion, and accelerate wound healing.

The invention creatively combines the six plant extracts of the feather cockscomb seed extract, the sage extract, the verbena extract, the rosemary extract, the witch hazel extract and the calendula extract, and the six ingredients supplement each other and have synergistic interaction, thereby having remarkable skin care and hair care effects for dogs. Specifically, the plant composition can effectively remove mites on the body surface of a dog, inhibit bacteria on the body surface of the dog, remarkably improve the skin microenvironment of the dog and reduce the occurrence of skin diseases of the dog; the skin oil secretion of the dog can be effectively balanced, and the occurrence of seborrheic dermatitis is reduced; can also improve the phenomenon of dry and unsmooth hair of dogs.

Preferably, the dog plant composition with skin and hair care effects comprises, by weight, 5-50 parts of a feather cockscomb seed extract, 5-50 parts of a sage extract, 5-10 parts of a verbena extract, 10-40 parts of a rosemary extract, 5-10 parts of a witch hazel extract and 20-30 parts of a calendula extract.

When the components in the plant composition for dogs are proportioned according to the specific weight, the plant composition for dogs plays more remarkable roles in removing mites, inhibiting bacteria, resisting inflammation, balancing grease, improving dryness and smoothness of hair and the like.

The parts by weight of the feather cockscomb seed extract can be 5 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts and the like, and other specific point values in the numerical range can be selected, so that the details are not repeated.

The weight parts of the sage extract can be 5 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts and the like, and other specific point values in the numerical range can be selected, so that the repeated description is omitted.

The rosemary extract can be 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts and the like by weight, and other specific point values in the numerical range can be selected, so that the description is omitted.

The weight parts of the witch hazel extract can be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like, and other specific point values in the numerical range can be selected, so that the repeated description is omitted.

The verbena extract can be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like by weight, and other specific point values in the numerical value range can be selected, so that the description is omitted.

The parts by weight of the calendula extract can be 20 parts, 22 parts, 23 parts, 25 parts, 27 parts, 30 parts and the like, and other specific point values in the numerical value range can be selected, so that the description is omitted.

The plant extracts in the plant composition for dogs can be directly purchased from commercial sources or prepared by self, and more preferably can be prepared by a method in which celosia seeds, salvia and verbena are extracted by water, and rosemary, witch hazel and calendula are extracted by alcohol.

Preferably, the feather cockscomb seed extract, salvia officinalis extract or verbena extract is independently prepared by a method comprising:

pulverizing semen Celosiae, herba Salvia officinalis or herba Verbenae, soaking in water, reflux extracting, concentrating the extractive solution, and oven drying.

Preferably, the mass ratio of the water to the feather cockscomb seed, salvia officinalis or verbena is (5-15):1, such as 5:1, 6:1, 7:1, 8:1, 10:1, 12:1, 13:1, 14:1, 15:1 and the like, and other specific point values in the numerical value range can be selected, so that the description is omitted.

Preferably, the soaking temperature is 15-30 deg.C, such as 15 deg.C, 17 deg.C, 20 deg.C, 22 deg.C, 24 deg.C, 25 deg.C, 27 deg.C, 28 deg.C, 30 deg.C, etc.; the time is 15-30h, for example, 15h, 17h, 20h, 22h, 24h, 25h, 27h, 30h, etc., and other specific point values within the above numerical range can be selected, which is not described herein again.

Preferably, the number of reflux extractions is 1-4, such as 1, 2, 3, 4; every time 50-90min, such as 50min, 60min, 70min, 80min, 90min, etc., other specific point values within the numerical range can be selected, and are not described in detail herein.

Preferably, the rosemary extract is prepared by a method comprising:

drying and pulverizing herba Rosmarini officinalis, boiling with 65-75% (such as 65%, 68%, 70%, 72%, 75%) ethanol, reflux extracting to obtain extractive solution, concentrating, and oven drying.

Preferably, the number of times of reflux leaching is 1-4, such as 1, 2, 3, 4; every time 20-40min, such as 20min, 25min, 30min, 35min, 40min, etc., other specific point values within the numerical range can be selected, and are not described in detail herein.

Preferably, the witch hazel extract is prepared by a method comprising:

drying and pulverizing Hamamelis virginiana, boiling with 55-65% (such as 55%, 58%, 60%, 62%, 65%) ethanol by volume, reflux extracting to obtain extractive solution, concentrating, and oven drying;

preferably, the number of times of reflux leaching is 1-4, such as 1, 2, 3, 4; every time 20-40min, such as 20min, 25min, 30min, 35min, 40min, etc., other specific point values within the numerical range can be selected, and are not described in detail herein.

Preferably, the calendula extract is prepared by a method comprising:

drying and pulverizing herba Sidae Rhombifoliae, boiling with 70-80% (such as 70%, 72%, 75%, 77%, 80%) ethanol by volume, reflux extracting to obtain extractive solution, concentrating, and oven drying;

preferably, the number of times of reflux leaching is 1-4, such as 1, 2, 3, 4; every time 20-40min, such as 20min, 25min, 30min, 35min, 40min, etc., other specific point values within the numerical range can be selected, and are not described in detail herein.

In a second aspect, the present invention provides the use of a canine plant composition having skin and hair care efficacy as described in the first aspect, in the preparation of a canine wash care product.

Preferably, the canine washing and caring product comprises canine shampoo, canine hair conditioner and canine washing and caring lotion.

Preferably, the content of the plant composition for dogs with skin and hair care effects in the washing care product for dogs is 0.1-15% by mass, such as 0.1%, 1%, 2%, 3%, 5%, 7%, 10%, 12%, 13%, 15%, etc., and other specific values in the numerical range can be selected, and are not repeated herein.

In a third aspect, the invention provides a canine shampoo with skin and hair care effects, which is prepared from the canine plant composition with skin and hair care effects, ecological nutrients, a surfactant, a chelating agent, a humectant, a thickener, a pH regulator, a preservative and water.

The shampoo for dogs, disclosed by the invention, is prepared by matching the plant composition and the ecological nutrients for dogs in the first aspect with other conventional additive components including a surfactant, a chelating agent, a humectant, a thickening agent, a pH regulator, a preservative and water, so that the shampoo has excellent cleaning and moisturizing effects, and also has the effects of removing mites on the body surface of dogs, inhibiting bacteria on the body surface of dogs, improving the skin microenvironment of dogs, reducing the occurrence of canine skin diseases, effectively balancing the grease secretion of the skin of dogs, reducing seborrheic dermatitis and improving the dryness and smoothness of the hair of dogs.

Preferably, the ecological nutrient comprises any one or a combination of at least two of inulin, alpha-glucan oligosaccharide, fructooligosaccharide, galactooligosaccharide, xylooligosaccharide, isomaltooligosaccharide or soybean oligosaccharide; the combination of at least two of the inulin and the alpha-glucan oligosaccharide, the alpha-glucan oligosaccharide and the fructo-oligosaccharide, the galacto-oligosaccharide and the xylo-oligosaccharide, and the like can be selected in any combination manner, and the details are not repeated herein.

The ecological nutrient can stimulate the growth of beneficial flora, but not harmful bacteria with potential pathogenicity or putrefactive activity, so that the probiotic microbiota of the pet can play a greater role, and the combined effect of inulin, alpha-glucan oligosaccharide, galacto-oligosaccharide and soybean oligosaccharide is optimal. The combination mode can assist in stabilizing the foam of the shower gel, has good dispersibility, spreadability and thickening effect, can make the foam fine and soft, is easy to wash and clean, has good skin feel, can promote the skin smoothness of pets and maintain hair, and has certain antioxidant and nutritional effects.

A combination of inulin, alpha-glucan oligosaccharides, galactooligosaccharides and soy oligosaccharides is preferred.

Preferably, the mass ratio of the inulin, the alpha-glucan oligosaccharide, the galactooligosaccharide and the soybean oligosaccharide is (2-3): (2-3):1 (1-2), such as 2:2:1:1, 2:2:1:2, 3:2:1:1, 3:2:1:2, 2:3:1:1, 2:3:1:2, 3:3:1:1, 3:3:1:2, and the like, and other specific points in the numerical value range can be selected, and are not described in detail herein.

Preferably, the surfactant comprises any one of sodium laureth sulfate, cocamide DEA, cocamide propyl betaine or lauryl glucoside, or a combination of at least two thereof. The combination of at least two of the above-mentioned compounds, such as the combination of sodium laureth sulfate and cocamide DEA, the combination of cocamide propyl betaine and lauryl glucoside, the combination of cocamide DEA and cocamide propyl betaine, etc., can be selected in any combination manner, and will not be described in detail herein.

Preferably, the humectant comprises any one or a combination of at least two of glycerol, propylene glycol, 1, 3-butylene glycol, sorbitol, polyethylene glycol, hyaluronic acid, ceramide or collagen; the combination of at least two of the above-mentioned compounds, such as the combination of glycerin and propylene glycol, the combination of 1, 3-butylene glycol and sorbitol, the combination of hyaluronic acid and ceramide, etc., can be selected in any combination manner, and will not be described in detail herein. Glycerol is preferred.

Preferably, the chelating agent comprises disodium EDTA.

Preferably, the thickener comprises sodium chloride.

Preferably, the pH adjusting agent comprises citric acid and/or sodium citrate.

The shampoo for dogs, provided by the invention, can be prepared by adopting a conventional technical means in the field, and can be exemplified by:

(1) mixing a chelating agent with water to obtain a first mixed solution;

(2) mixing the first mixed solution obtained in the step (1) with a surfactant to obtain a second mixed solution;

(3) and (3) mixing the second mixed solution obtained in the step (2), the canine plant composition, the ecological nutrient, the humectant, the thickener, the pH regulator and the preservative to obtain the feed additive.

Preferably, the mixing temperature in step (1) is 80-85 ℃, for example 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃, 85 ℃ and the like, and the mixing time is 5-10min, for example 5min, 6min, 7min, 8min, 9min, 10min and the like, and specific point values in the above numerical value ranges can be selected, and are not described in detail herein.

Preferably, the mixing temperature in step (2) is 60-70 ℃, such as 60 ℃, 62 ℃, 64 ℃, 65 ℃, 66 ℃, 68 ℃, 70 ℃ and the like, and the mixing time is 5-15min, such as 5min, 6min, 7min, 8min, 10min, 12min, 15min and the like, and specific values within the above numerical ranges can be selected, which is not described in detail herein.

Preferably, the mixing temperature in step (3) is 40-50 ℃, for example, 40 ℃, 42 ℃, 44 ℃, 45 ℃, 46 ℃, 48 ℃, 50 ℃ and the like, the mixing time is 20-40min, for example, 20min, 22min, 25min, 30min, 32min, 35min, 40min and the like, and specific values within the above numerical ranges can be selected, which is not described in detail herein.

Compared with the prior art, the invention has the following beneficial effects:

the invention creatively combines the six plant extracts of the feather cockscomb seed extract, the sage extract, the verbena extract, the rosemary extract, the witch hazel extract and the calendula extract, and the six ingredients supplement each other and have synergistic interaction, thereby having remarkable skin care and hair care effects for dogs. Specifically, the plant composition can effectively remove mites on the body surface of a dog, inhibit bacteria on the body surface of the dog, remarkably improve the skin microenvironment of the dog and reduce the occurrence of skin diseases of the dog; the skin oil secretion of the dog can be effectively balanced, and the occurrence of seborrheic dermatitis is reduced; can also improve the phenomenon of dry and unsmooth hair of dogs.

Detailed Description

To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

The following examples 1 to 3, comparative examples 1 to 6, and application examples relate to the following raw material sources:

raw materials	Manufacturer of the product	Number plate
			Semen Celosiae extract	Xian Youshou Biotech Co., Ltd	YS-170
Salvia officinalis extract	Western securityOlympic science and technology Co Ltd	QA-930
			Verbena extract	Saishu Olympic Biotechnology Ltd	QA-398
Rosemary extract	Saishu Olympic Biotechnology Ltd	MDX-10
			Hamamelis mollis extract	Saishu Olympic Biotechnology Ltd	JLM-10
Calendula extract	Saishu Olympic Biotechnology Ltd	QA-208
			Inulin powder	SHAANXI HUIKE BOTANICAL DEVELOPMENT Co.,Ltd.	Inulin 90%
Alpha-glucan oligosaccharides	Guangzhou Jinbaolai Biotechnology Ltd	EFFECT KGT100
			Galacto-oligosaccharides	NEW FRANCISCO (YUNFU) BIOTECHNOLOGY Corp.	GOS-270-P
Soybean oligosaccharide	Yihe-Yuan Biotechnology Ltd of Jiangsu	99 percent of soybean oligosaccharide
			Fructo-oligosaccharide	Orafti Belgium	Orafti P95
Xylo-oligosaccharide	SHANDONG LONGLIVE BIO-TECHNOLOGY Co.,Ltd.	XOS-95
			Cocoamidopropyl betaine	KEYU NEW MATERIALS Co.,Ltd.	CAB-35

Other preparation raw materials can also be purchased from commercial sources.

Preparation example 1

The preparation example provides a feather cockscomb seed extract, and the preparation method comprises the following steps:

pulverizing semen Celosiae, mixing with 10 times of double distilled water, soaking at 25 deg.C for 60min, reflux-extracting for 70min for 2 times, concentrating the extractive solution, and oven drying.

Preparation example 2

The preparation example provides a sage extract, and the preparation method comprises the following steps:

pulverizing herba Salvia officinalis, mixing with 15 times of double distilled water, soaking at 30 deg.C for 60min, reflux extracting for 3 times, each for 50min, concentrating the extractive solution, and oven drying.

Preparation example 3

The preparation example provides a verbena extract, and the preparation method comprises the following steps:

pulverizing herba Verbenae, mixing with 15 times of double distilled water, soaking at 30 deg.C for 60min, reflux extracting for 2 times (each time for 80 min), concentrating the extractive solution, and oven drying.

Preparation example 4

The preparation example provides a rosemary extract, and the preparation method comprises the following steps:

pulverizing herba Rosmarini officinalis, mixing with 10 times of 70% ethanol water solution, heating to boil, reflux extracting for 3 times (30 min each time), concentrating the extractive solution, and oven drying.

Preparation example 5

The preparation example provides a witch hazel extract, and the preparation method comprises the following steps:

pulverizing Hamamelis mollis, mixing with 10 times of 60% ethanol water solution, heating to boil, reflux extracting for 3 times, each for 30min, concentrating the extractive solution, and oven drying.

Preparation example 6

The preparation example provides a calendula extract, and the preparation method comprises the following steps:

pulverizing herba Sidae Rhombifoliae, mixing with 10 times of 75% ethanol water solution by volume, heating to boil, reflux extracting for 3 times, each for 30min, concentrating the extractive solution, and oven drying.

Example 1

The embodiment provides a dog plant composition with skin and hair care effects, which consists of 30 parts of feather cockscomb seed extract, 20 parts of sage extract, 10 parts of verbena extract, 20 parts of rosemary extract, 5 parts of witch hazel extract and 20 parts of calendula extract. The preparation method comprises the following steps: the above materials are mixed uniformly according to a certain proportion.

Example 2

The embodiment provides a dog plant composition with skin and hair care effects, which consists of 5 parts of feather cockscomb seed extract, 50 parts of sage extract, 5 parts of verbena extract, 10 parts of rosemary extract, 5 parts of witch hazel extract and 30 parts of calendula extract. The preparation method comprises the following steps: the above materials are mixed uniformly according to a certain proportion.

Example 3

The embodiment provides a dog plant composition with skin and hair care effects, which consists of 50 parts of feather cockscomb seed extract, 10 parts of sage extract, 10 parts of verbena extract, 10 parts of rosemary extract, 5 parts of witch hazel extract and 20 parts of calendula extract. The preparation method comprises the following steps: the above materials are mixed uniformly according to a certain proportion.

Example 4

This example provides a canine plant composition having skin and hair care efficacy, which differs from example 1 in that: commercially available semen Celosiae extract, herba Salvia officinalis extract, herba Verbenae extract, herba Rosmarini officinalis extract, radix Hamamelis mollis extract and herba Sidae Rhombifoliae extract are respectively replaced with the products prepared in preparation examples 1-6, and their mixture ratio is kept unchanged. The preparation method comprises the following steps: the above materials are mixed uniformly according to a certain proportion.

Comparative example 1

This comparative example provides a canine plant composition having skin and hair care efficacy, which differs from example 1 in that: the feather cockscomb seed extract is absent, and the feather cockscomb seed extract is distributed with reduced fraction on the sage extract, verbena extract, rosemary extract, witch hazel extract, calendula extract in weight ratio of other extracts. The preparation process is as in example 1.

Comparative example 2

This comparative example provides a canine plant composition having skin and hair care efficacy, which differs from example 1 in that: lacking the sage extract and distributing the reduced fraction of the sage extract on the celosia seed extract, verbena extract, rosemary extract, witch hazel extract, calendula extract in weight ratio of the other extracts. The preparation process is as in example 1.

Comparative example 3

This comparative example provides a canine plant composition having skin and hair care efficacy, which differs from example 1 in that: the verbena extract is absent and the reduced fraction of the verbena extract is distributed on the celosia seed extract, the sage extract, the rosemary extract, the witch hazel extract, the calendula extract in the weight ratio of the other extracts. The preparation process is as in example 1.

Comparative example 4

This comparative example provides a canine plant composition having skin and hair care efficacy, which differs from example 1 in that: the rosemary extract is absent and a reduced fraction of rosemary extract is distributed by weight ratio to the other extracts on feather cockscomb seed extract, sage extract, verbena extract, witch hazel extract, calendula extract. The preparation process is as in example 1.

Comparative example 5

This comparative example provides a canine plant composition having skin and hair care efficacy, which differs from example 1 in that: the hamamelis extract is absent and the reduced fraction of the hamamelis extract is distributed on the celosia seed extract, sage extract, verbena extract, rosemary extract, calendula extract in the weight ratio of the other extracts. The preparation process is as in example 1.

Comparative example 6

This comparative example provides a canine plant composition having skin and hair care efficacy, which differs from example 1 in that: the calendula extract is absent and the reduced fraction of calendula extract is distributed on the celosia seed extract, sage extract, verbena extract, rosemary extract, witch hazel extract in the weight ratio of the other extracts. The preparation process is as in example 1.

Application example 1

The application example provides a shampoo for dogs with skin and hair care effects, which comprises the following components in percentage by weight:

10% of the canine plant composition of example 1; inulin 0.3%, alpha-glucan oligosaccharide 0.2%, galacto-oligosaccharide 0.1%, soybean oligosaccharide 0.1%; 6% of cocamidopropyl betaine; 0.1% of EDTA disodium; 3% of glycerol; 1% of NaCl; 0.1% of citric acid; 0.1 percent of methyl hydroxybenzoate and the balance of water.

The preparation method comprises the following steps:

(1) mixing EDTA disodium and water at 80 ℃ for 10min to obtain a first mixed solution;

(2) mixing the first mixed solution obtained in the step (1) and cocamidopropyl betaine at 65 ℃ for 10min to obtain a second mixed solution;

(3) and (3) mixing the second mixed solution obtained in the step (2), the dog plant composition obtained in the example 1, inulin, alpha-glucan oligosaccharide, galacto-oligosaccharide, soybean oligosaccharide, glycerol, NaCl, citric acid and methyl hydroxybenzoate at 45 ℃ for 30min to obtain the dog shampoo with skin and hair care effects.

Application examples 2 to 4

This application example provides three types of canine shampoos having skin and hair care effects, the components of which differ from those of application example 1 only in that the canine plant composition of example 1 was replaced with the canine plant compositions of examples 2 to 4, and the other conditions were maintained. The preparation method is the same as in application example 1.

Comparative application examples 1 to 6

This application example provides six kinds of shampoos for dogs having skin and hair care effects, the components of which are different from those of application example 1 only in that the plant composition for dogs of example 1 is replaced with the plant composition for dogs of comparative examples 1 to 6, and other conditions are maintained unchanged. The preparation method is the same as in application example 1.

Comparative application examples 7 to 12

The application example provides six kinds of dog shampoos with skin and hair care effects, the components of which are different from those of the application example 1 only in that the dog plant composition of the example 1 is respectively replaced by a single feather cockscomb seed extract, a sage extract, a verbena extract, a rosemary extract, a witch hazel extract and a calendula extract in sequence, and other conditions are kept unchanged. The preparation method is the same as in application example 1.

Application example 5

The application example provides a shampoo for dogs with skin and hair care effects, which is only different from the application example 1 in that the ecological nutrients comprise the following components: 0.3% of alpha-glucan oligosaccharide, 0.2% of galacto-oligosaccharide and 0.2% of soybean oligosaccharide; all other conditions remained unchanged. The preparation method is the same as in application example 1.

Application example 6

The application example provides a shampoo for dogs with skin and hair care effects, which is only different from the application example 1 in that the ecological nutrients comprise the following components: inulin 0.3%, galacto-oligosaccharide 0.2%, soybean oligosaccharide 0.2%; all other conditions remained unchanged. The preparation method is the same as in application example 1.

Application example 7

The application example provides a shampoo for dogs with skin and hair care effects, which is only different from the application example 1 in that the ecological nutrients comprise the following components: inulin 0.3%, alpha-glucan oligosaccharide 0.2%, soybean oligosaccharide 0.2%; all other conditions remained unchanged. The preparation method is the same as in application example 1.

Application example 8

The application example provides a shampoo for dogs with skin and hair care effects, which is only different from the application example 1 in that the ecological nutrients comprise the following components: inulin 0.3%, alpha-glucan oligosaccharide 0.2%, and galacto-oligosaccharide 0.2%; all other conditions remained unchanged. The preparation method is the same as in application example 1.

Test example 1:

the safety performance test was performed on the shampoo for dogs provided in application examples 1 to 8, and the method was as follows:

preparation of erythrocyte suspension: selecting healthy rabbits, taking 9mL of blood from heart, adding 1mL of 2% potassium oxalate solution, centrifuging, discarding supernatant, diluting the precipitate to 20mL with 20mmol/L PBS solution, and storing at 4 ℃ for later use. Select samples were diluted with PBS solution to different concentrations, with 5 concentration gradients set for each sample. Adding 200 μ L of the above erythrocyte suspension (final concentration of the sample is controlled to be 5, 10, 20, 50, 100mg/mL respectively) into 10mL of diluent of the sample to be tested, taking distilled water as total blood-dissolving control, taking PBS solution as negative control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting supernatant, and testing its absorbance at 560nm with spectrophotometer (A)₅₆₀) Calculating the hemolysis rate according to the following formula;

a standard curve of hemolysis rate vs. sample concentration was plotted, and the sample concentration at which hemolysis occurred in 50% erythrocytes (HD) was calculated₅₀)。

Diluting the sample to 10g/L with PBS solution, collecting 10mL dilution of the sample to be tested, adding 200 μ L of the erythrocyte suspension, using distilled water as blank control, 1mg/mL Sodium Dodecyl Sulfate (SDS) solution as positive control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting supernatant, and testing absorbance A at 540nm and 575nm with spectrophotometer₅₄₀And A₅₇₅Calculating a protein Denaturation Index (DI) according to the following formula;

wherein R is₁Blank control group a₅₇₅Blank control group A₅₄₀，R₂Experimental group a₅₇₅Experimental group A₅₄₀，R₃Positive control group A₅₇₅Positive control group A₅₄₀。

Evaluating the irritation of the sample to be tested according to the L/D value, wherein the L/D value is HD₅₀DI, erythrocyte hemolysis assay irritation grading criteria are shown in Table 1 below:

TABLE 1

L/D	Grading
		>100	Has no irritation
10<L/D≤100	Micro-stimulation property
		1<L/D≤10	Mild irritation
0.1<L/D≤1	Moderate irritation

The test results are shown in table 2 below:

TABLE 2

Test sample	L/D	Evaluation of
			Application example 1	>100	Has no irritation
Application example 2	>100	Has no irritation
			Application example 3	>100	Has no irritation
Application example 4	>100	Has no irritation
			Application example 5	>100	Has no irritation
Application example 6	>100	Has no irritation
			Application example 7	>100	Has no irritation
Application example 8	>100	Has no irritation

As can be seen from the data in Table 2: the plant composition for dogs and the shampoo for dogs, provided by the invention, are mild, have no stimulation and are high in safety.

Test example 2:

the evaluation of the antibacterial effect of the shampoo for dogs provided in the application examples 1 to 8 and the comparative application examples 1 to 12 was carried out by the following method:

the test is carried out by referring to the method for testing the antibacterial effect of the antibacterial daily chemical products in QB/T2738-. Results of bacteriostatic rate are shown in table 3:

TABLE 3

The plant composition for dogs and the shampoo for dogs have remarkable antibacterial effect, the sage extract, the witch hazel extract and the calendula extract in the formula play main roles, and the six plant extracts have a certain synergistic effect on the antibacterial effect.

Test example 3:

the evaluation of mite-removing effect was carried out on the shampoo for dogs provided in application examples 1 to 8 and comparative application examples 1 to 12 by the following method:

the mite control rate was calculated by referring to NY/T1151.2-2006, and the results are shown in Table 4:

TABLE 4

From the results in Table 4, it can be seen that: the plant composition for dogs and the shampoo for dogs have obvious acarus killing effect, the sage extract and the verbena extract in the formula play main roles, and the six plant extracts have a certain synergistic effect on the acarus killing effect.

Test example 4:

evaluation of hair moisturizing effect was performed on the shampoo for dogs provided in application examples 1 to 8 and comparative application examples 1 to 12 by the following method:

the moisturizing effect of the product on hair is measured by a gravimetric method: the dorsal hair of a healthy york summer fur dog was selected as a sample. The shampoo for dogs of application examples 1-8 and comparative application examples 1-12 was used to clean for 5min and then rinsed with clear water for 1 min. Then putting the mixture into an oven to dry for 24 hours at the temperature of 30 ℃.

Putting the sample into a drying dish of which the humidity is strictly controlled to be 80% and saturated KCl solution for 12 hours, and weighing the mass m₁Then, thenThe sample is placed in saturated CaCl₂After the solution is strictly controlled in a drying dish with the humidity of 26% for 12 hours, the mass m is weighed₂And finally calculating the water loss R. R ═ m₁-m₂)/m₁X 100%. In the present method, a smaller hair moisture loss amount R indicates a better moisturizing ability. The results are shown in Table 5:

TABLE 5

From the results in Table 5, it can be seen that: the plant composition for dogs and the shampoo for dogs have obvious moisturizing effect on dog hair, the six plant extracts in the formula have a certain synergistic effect on the exertion of the moisturizing effect, and ecological nutrients in the formula also have great influence on the moisturizing effect.

Test example 5:

evaluation of hair antistatic effect was performed on the shampoo for dogs provided in application examples 1 to 8 and comparative application examples 1 to 12 by the following method:

referring to GB/T16801, the antistatic effect of hair is tested by the surface specific resistance method: the dorsal hair of a healthy york summer fur dog was selected as a sample. The shampoo for dogs of application examples 1-8 and comparative application examples 1-12 was used to clean for 5min and then rinsed with clear water for 1 min. Then putting the mixture into an oven to dry for 24 hours at the temperature of 30 ℃. In the test, the temperature was 20 ℃ and the relative humidity was 26%. The antistatic property, namely the resistance value Deltalg rhos, was measured. The lower this value, the lower the resistance, the better the antistatic properties. The results are shown in Table 6:

TABLE 6

From the results in Table 6, it can be seen that: the plant composition for dogs and the shampoo for dogs have obvious antistatic effect on dog hair, the six plant extracts in the formula have certain synergistic effect on the exertion of the antistatic effect, and ecological nutrients in the formula also have great influence on the antistatic effect.

The applicant states that the present invention is described in the above examples to describe a plant composition for dogs having skin and hair care effects and its application, but the present invention is not limited to the above examples, i.e., it does not mean that the present invention must be implemented by the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.

It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.