阅读说明：本技术 用于供体器官上的碳水化合物抗原切割的酶促组合物、与其相关的方法和用途 (Enzymatic compositions for carbohydrate antigen cleavage on donor organs, methods and uses related thereto ) 是由 马塞洛·塞普拉 王艾舟 沙菲克·克沙夫吉 斯蒂芬·G·威瑟斯 彼得·拉费尔德 加雅善德兰· 于 2019-08-16 设计创作，主要内容包括：本文提供了用于酶促切割来自供体器官的A抗原的灌注流体,以及与其相关的方法、用途。具体地,所述灌注流体包含两种酶GalNAc脱乙酰酶和半乳糖胺酶,并且所述流体还可以包含缓冲的细胞外溶液和/或拥挤剂。此外,发现本文所述的组合物在适于细胞存活的温度和pH水平下具有活性。(Provided herein are perfusion fluids for enzymatic cleavage of a antigen from a donor organ, and methods, uses related thereto. In particular, the perfusion fluid comprises the two enzymes GalNAc deacetylase and galactosaminase, and the fluid may also comprise a buffered extracellular solution and/or a crowding agent. In addition, the compositions described herein were found to be active at temperatures and pH levels suitable for cell survival.)