阅读说明：本技术 一种防治稻飞虱的植物源农药的方法 (Method for preparing botanical pesticide for preventing and treating rice planthoppers ) 是由 龚浩如 陶曙华 赵杨 陈英姿 陈祖武 苗雪雪 王洁敏 陈彦成 胡利斌 汪琛 于 2021-02-05 设计创作，主要内容包括：本发明公开了一种防治稻飞虱的植物源农药的方法,防治稻飞虱的植物源农药的制备方法包括将海藻糖、壳聚糖和和余量的中药组分均匀混合,制成含中药活性混合培养基；将复合微生物菌株接种到含中药活性混合培养基进行发酵培养,即得到防治稻飞虱的植物源农药,采用了采用的走马芹、狼毒、地肤子、白头翁、苦参、黎芦、苍耳、蛇床子和丁香能够起到有效的除螨作用；同时,海藻酸钠和壳聚糖能够提高复合微生物菌的活性,而这些微生物是稻飞虱的天然杀手,因此中药与微生物的协同使用能够实现无公害地有效防治稻飞虱。(The invention discloses a method for preparing a botanical pesticide for preventing and treating rice planthoppers, which comprises the steps of uniformly mixing trehalose, chitosan and the balance of traditional Chinese medicine components to prepare a mixed culture medium containing traditional Chinese medicine activity; inoculating the compound microorganism strain to a mixed culture medium containing traditional Chinese medicine activity for fermentation culture to obtain the botanical pesticide for preventing and treating rice planthopper, wherein the adopted marchantia polymorpha, radix euphorbiae lantu, fructus kochiae, Chinese pulsatilla root, radix sophorae flavescentis, radix rhapontici, xanthium sibiricum, fructus cnidii and clove can play an effective mite removing role; meanwhile, the sodium alginate and the chitosan can improve the activity of the compound microorganisms which are natural killers of the rice planthoppers, so that the synergistic use of the traditional Chinese medicines and the microorganisms can realize the pollution-free effective control of the rice planthoppers.)

1. A method for preparing a botanical pesticide for controlling rice planthoppers is characterized by comprising the following steps: the preparation method of the botanical pesticide for preventing and treating the rice planthoppers comprises the following steps:

s1: mixing trehalose 2-3 vol%, chitosan 0.4-0.6 vol% and the rest Chinese medicinal materials to obtain mixed culture medium containing Chinese medicinal activity;

s2: inoculating the compound microorganism strains to a mixed culture medium containing traditional Chinese medicine activity for fermentation culture until the concentration of the microorganisms reaches 30 hundred million strains/ml, and obtaining the botanical pesticide for preventing and treating the rice planthopper.

2. The method of controlling plant-derived pesticides of rice planthoppers according to claim 1, wherein said method comprises the steps of: the Chinese medicinal components in the step S1 comprise: herba Oenanthes Javanicae, radix Euphorbiae Fischerianae, Kochiae fructus, radix Pulsatillae, radix Sophorae Flavescentis, radix Trigonellae, herba Xanthii, fructus Cnidii and flos Caryophylli.

3. The method of controlling plant-derived pesticides of rice planthoppers according to claim 2, wherein: the traditional Chinese medicine comprises the following components in parts by weight: 4-6 parts of common fennel fruit; 4-8 parts by weight of radix euphorbiae lantu; 3-6 parts of fructus kochiae; 4-6 parts of Chinese pulsatilla root; 4-7 parts by weight of sophora flavescens; 3-6 parts of veratrum; 2-4 parts of xanthium sibiricum; 2-6 parts of fructus cnidii; 3-5 parts of clove.

4. The method of controlling plant-derived pesticides of rice planthoppers according to claim 1, wherein said method comprises the steps of: the compound microorganism bacterium in the step S2 is prepared by mixing bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, bacillus coagulans, aspergillus oryzae, rhizopus oryzae, paecilomyces lilacinus, destruxins destructor, lactobacillus plantarum and lactobacillus acidophilus in equal proportion.

Technical Field

The invention relates to the field of pesticides, in particular to a method for preparing a botanical pesticide for preventing and treating rice planthoppers.

Background

In recent years, the public nuisance of agricultural chemicals has attracted much attention from countries around the world, and low-toxicity and low-residue agricultural chemicals have been the focus of research. Biopesticides can control pests and weeds or improve ecology by directly utilizing interactions between biological substances and organisms in the nature, and thus biopesticides are internationally recognized as pesticides with the greatest development prospects. However, in the prior art, there are few kinds of biopesticides and no biopesticides capable of controlling rice planthoppers. Chemical pesticides play an important role in the process of preventing and treating forest pests, however, the environmental pollution and the crop harm caused by the chemical pesticides are also immeasurable. If biological agents are properly adopted for prevention and treatment, the materials are used locally, so that the harm caused by pollution can be avoided, and the prevention and treatment are timely, low in cost and good in effect. The soil pesticide plant resources for preventing and controlling the pests are abundant in various regions, and the soil pesticide plant resources are developed fully and reasonably to prevent and control the forest pests and achieve the aims of treating both symptoms and root causes.

Disclosure of Invention

The invention aims to provide a method for preparing a plant-derived pesticide for controlling rice planthoppers, which aims to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme:

a method for preparing a plant-derived pesticide for controlling rice planthoppers, which comprises the following steps:

s1: mixing trehalose 2-3 vol%, chitosan 0.4-0.6 vol% and the rest Chinese medicinal materials to obtain mixed culture medium containing Chinese medicinal activity;

s2: inoculating the compound microorganism strains to a mixed culture medium containing traditional Chinese medicine activity for fermentation culture until the concentration of the microorganisms reaches 30 hundred million strains/ml, and obtaining the botanical pesticide for preventing and treating the rice planthopper.

As a still further scheme of the invention: the Chinese medicinal components in the step S1 comprise: herba Oenanthes Javanicae, radix Euphorbiae Fischerianae, Kochiae fructus, radix Pulsatillae, radix Sophorae Flavescentis, radix Trigonellae, herba Xanthii, fructus Cnidii and flos Caryophylli.

As a still further scheme of the invention: the traditional Chinese medicine comprises the following components in parts by weight: 4-6 parts of common fennel fruit; 4-8 parts by weight of radix euphorbiae lantu; 3-6 parts of fructus kochiae; 4-6 parts of Chinese pulsatilla root; 4-7 parts by weight of sophora flavescens; 3-6 parts of veratrum; 2-4 parts of xanthium sibiricum; 2-6 parts of fructus cnidii; 3-5 parts of clove.

As a still further scheme of the invention: the compound microorganism bacterium in the step S2 is prepared by mixing bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, bacillus coagulans, aspergillus oryzae, rhizopus oryzae, paecilomyces lilacinus, destruxins destructor, lactobacillus plantarum and lactobacillus acidophilus in equal proportion.

Compared with the prior art, the invention has the beneficial effects that:

the botanical pesticide for preventing and treating rice planthopper adopts the adopted common fennel, radix euphorbiae lantu, fructus kochiae, Chinese pulsatilla, radix sophorae flavescentis, veratrum, xanthium sibiricum, fructus cnidii and clove, and can play an effective mite removing role; meanwhile, the sodium alginate and the chitosan can improve the activity of the compound microorganisms which are natural killers of the rice planthoppers, so that the synergistic use of the traditional Chinese medicines and the microorganisms can realize the pollution-free effective control of the rice planthoppers.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the experimental control group in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The first embodiment is as follows:

a method for preparing a botanical pesticide for controlling rice planthoppers comprises the following steps: cutting herba Oenanthes Javanicae root, decocting in water for 2 hr, filtering, decocting for 1 hr to obtain stock solution, adding water 1kg per kg, and weighing according to 4 weight parts; cleaning and chopping 1kg of radix Euphorbiae Fischerianae, adding 6kg of water, decocting for 1.5h, filtering, adding 5 times of water for dilution when in use, and weighing according to 4 parts by weight; weighing belvedere fruit according to 3 parts by weight; 1kg of Chinese pulsatilla root, 10kg of water is added, boiling is carried out for 0.5h or soaking is carried out for 1d, filtering is carried out, and the filtrate is weighed according to 4 parts by weight; 6kg of radix sophorae flavescentis, 10kg of water is added, dregs are removed after decoction, and the radix sophorae flavescentis is weighed according to 4 parts by weight; 1 part of veratrum, 5 parts of slaked lime are added, and the mixture is weighed according to 3 parts by weight after being uniformly mixed; mashing fructus Xanthii, adding 5kg of water per kg, soaking for 24h, and weighing according to 2 parts by weight; weighing the fructus cnidii and the clove according to 2 and 3 parts by weight respectively; the weighed components are fully and uniformly mixed. Uniformly mixing 2% of trehalose, 0.4% of chitosan and the balance of traditional Chinese medicine components to prepare a mixed culture medium containing traditional Chinese medicine activity; inoculating the compound microorganism strains to a mixed culture medium containing traditional Chinese medicine activity for fermentation culture until the concentration of the microorganisms reaches 30 hundred million strains/ml, and obtaining the botanical pesticide for preventing and treating the rice planthopper.

Example two:

a method for preparing a botanical pesticide for controlling rice planthoppers comprises the following steps: cutting herba Oenanthes Javanicae root, decocting in water for 2 hr, filtering, decocting for 1 hr to obtain stock solution, adding water 1kg per kg, and weighing according to 5 weight parts; cleaning and chopping 1kg of radix Euphorbiae Fischerianae, adding 6kg of water, decocting for 1.5h, filtering, adding 5 times of water for dilution when in use, and weighing according to 5 parts by weight; weighing belvedere fruit according to 4 parts by weight; 1kg of Chinese pulsatilla root, 10kg of water is added, boiling is carried out for 0.5h or soaking is carried out for 1d, filtering is carried out, and the filtrate is weighed according to 5 parts by weight; 6kg of radix sophorae flavescentis, 10kg of water is added, dregs are removed after decoction, and the radix sophorae flavescentis is weighed according to 5 parts by weight; 1 part of veratrum, 5 parts of slaked lime are added, and the mixture is weighed according to 4 parts by weight after being uniformly mixed; mashing fructus xanthil, adding 5kg of water into each kg of fructus xanthil, soaking for 24h, and weighing according to 3 parts by weight; weighing the fructus cnidii and the clove according to 3 and 4 parts by weight respectively; the weighed components are fully and uniformly mixed. Uniformly mixing 2% of trehalose, 0.4% of chitosan and the balance of traditional Chinese medicine components to prepare a mixed culture medium containing traditional Chinese medicine activity; inoculating the compound microorganism strains to a mixed culture medium containing traditional Chinese medicine activity for fermentation culture until the concentration of the microorganisms reaches 30 hundred million strains/ml, and obtaining the botanical pesticide for preventing and treating the rice planthopper.

Example three:

a method for preparing a botanical pesticide for controlling rice planthoppers comprises the following steps: cutting herba Oenanthes Javanicae root, decocting in water for 2 hr, filtering, decocting for 1 hr to obtain stock solution, adding water 1kg per kg, and weighing according to 6 weight parts; cleaning and chopping 1kg of radix Euphorbiae Fischerianae, adding 6kg of water, decocting for 1.5h, filtering, adding 5 times of water for dilution when in use, and weighing according to 6 parts by weight; weighing belvedere fruit according to 5 parts by weight; 1kg of Chinese pulsatilla root, 10kg of water is added, boiling is carried out for 0.5h or soaking is carried out for 1d, filtering is carried out, and the filtrate is weighed according to 6 parts by weight; 6kg of radix sophorae flavescentis, 10kg of water is added, dregs are removed after decoction, and the radix sophorae flavescentis is weighed according to 6 parts by weight; 1 part of veratrum, 5 parts of slaked lime are added, and the mixture is weighed according to 5 parts by weight after being uniformly mixed; mashing fructus Xanthii, adding 5kg of water per kg, soaking for 24h, and weighing according to 4 parts by weight; weighing fructus Cnidii and flos Caryophylli respectively according to 4 and 5 weight parts; the weighed components are fully and uniformly mixed. Uniformly mixing 2% of trehalose, 0.4% of chitosan and the balance of traditional Chinese medicine components to prepare a mixed culture medium containing traditional Chinese medicine activity; inoculating the compound microorganism strains to a mixed culture medium containing traditional Chinese medicine activity for fermentation culture until the concentration of the microorganisms reaches 30 hundred million strains/ml, and obtaining the botanical pesticide for preventing and treating the rice planthopper.

Example four:

a method for preparing a botanical pesticide for controlling rice planthoppers comprises the following steps: cutting herba Oenanthes Javanicae root, decocting in water for 2 hr, filtering, decocting for 1 hr to obtain stock solution, adding water 1kg per kg, and weighing according to 5 weight parts; cleaning and chopping 1kg of radix Euphorbiae Fischerianae, adding 6kg of water, decocting for 1.5h, filtering, adding 5 times of water for dilution when in use, and weighing according to 7 parts by weight; weighing belvedere fruit according to 6 parts by weight; 1kg of Chinese pulsatilla root, 10kg of water is added, boiling is carried out for 0.5h or soaking is carried out for 1d, filtering is carried out, and the filtrate is weighed according to 6 parts by weight; 6kg of radix sophorae flavescentis, 10kg of water is added, dregs are removed after decoction, and the radix sophorae flavescentis is weighed according to 7 parts by weight; 1 part of veratrum, 5 parts of slaked lime are added, and the mixture is weighed according to 6 parts by weight after being uniformly mixed; mashing fructus Xanthii, adding 5kg of water per kg, soaking for 24h, and weighing according to 4 parts by weight; weighing the fructus cnidii and the clove according to 5 and 5 parts by weight respectively; the weighed components are fully and uniformly mixed. Uniformly mixing 2% of trehalose, 0.4% of chitosan and the balance of traditional Chinese medicine components to prepare a mixed culture medium containing traditional Chinese medicine activity; inoculating the compound microorganism strains to a mixed culture medium containing traditional Chinese medicine activity for fermentation culture until the concentration of the microorganisms reaches 30 hundred million strains/ml, and obtaining the botanical pesticide for preventing and treating the rice planthopper.

Example five:

cutting herba Oenanthes Javanicae root, decocting in water for 2 hr, filtering, decocting for 1 hr to obtain stock solution, adding water 1kg per kg, and weighing according to 6 weight parts; cleaning and chopping 1kg of radix Euphorbiae Fischerianae, adding 6kg of water, decocting for 1.5h, filtering, adding 5 times of water for dilution when in use, and weighing according to 8 parts by weight; weighing belvedere fruit according to 6 parts by weight; 1kg of Chinese pulsatilla root, 10kg of water is added, boiling is carried out for 0.5h or soaking is carried out for 1d, filtering is carried out, and the filtrate is weighed according to 6 parts by weight; 6kg of radix sophorae flavescentis, 10kg of water is added, dregs are removed after decoction, and the radix sophorae flavescentis is weighed according to 7 parts by weight; 1 part of veratrum, 5 parts of slaked lime are added, and the mixture is weighed according to 6 parts by weight after being uniformly mixed; mashing fructus Xanthii, adding 5kg of water per kg, soaking for 24h, and weighing according to 4 parts by weight; weighing fructus Cnidii and flos Caryophylli respectively according to 6 and 5 weight parts; the weighed components are fully and uniformly mixed. Uniformly mixing 2% of trehalose, 0.4% of chitosan and the balance of traditional Chinese medicine components to prepare a mixed culture medium containing traditional Chinese medicine activity; inoculating the compound microorganism strains to a mixed culture medium containing traditional Chinese medicine activity for fermentation culture until the concentration of the microorganisms reaches 30 hundred million strains/ml, and obtaining the botanical pesticide for preventing and treating the rice planthopper.

Setting a control group, averagely dividing the pest area into 7 test areas, wherein the test area 1 is the control group, and the test area 1 is sprayed with common pesticide; after the plant-derived pesticides of examples 1 to 5 were sprayed for 3 days in the test areas 2 to 6, respectively, the results are as follows:

group of	Insecticidal effect (3d)	Residual ovum after 30 days
			Control group	81.55％	9.43％
Example 1	97.36％	4.23％
			Example 2	96.76％	3.23％
Example 3	98.88％	3.26％
			Example 4	95.43％	1.98％
Example 5	96.58％	2.91％

As can be seen from the table above, the invention has good insecticidal effect and durable pesticide effect, and the rice planthopper is not easy to generate resistance after being sprayed

In addition, the insecticidal effect is better.

By combining the above analysis, it can be confirmed that the present invention can effectively control rice planthoppers without public nuisance.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.