阅读说明：本技术 鸡骨香乙醇部提取物在制备用于舒张预收缩的气管平滑肌药物中的应用 (Application of chicken bone fragrant ethanol part extract in preparing medicine for relaxing and pre-contracting tracheal smooth muscle ) 是由 沈金花 姚凤杰 于 2021-01-27 设计创作，主要内容包括：本发明涉及医药技术领域,尤其涉及鸡骨香乙醇部提取物在制备用于舒张预收缩的气管平滑肌的药物中的应用。本发明通过对实验小鼠离体气管环肌张力的检测,发现鸡骨香乙醇部提取物具有高效的舒张气管平滑肌的作用,提示此物质有望应用于缓解哮喘。为开发鸡骨香乙醇部提取物在制备用于舒张预收缩的气管平滑肌的药物中的应用提供了依据。(The invention relates to the technical field of medicines, in particular to application of an extract of an ethanol part of chicken bone in preparing a medicine for relaxing pre-contracted tracheal smooth muscle. According to the invention, through detecting the tension of isolated tracheal ring muscle of an experimental mouse, the chicken bone aroma ethanol part extract is found to have an efficient effect of relaxing tracheal smooth muscle, and the substance is expected to be applied to relieving asthma. Provides a basis for developing the application of the extract of the chicken bone fragrant ethanol part in preparing the medicine for relaxing the pre-contracted tracheal smooth muscle.)

1. Use of an extract of the ethanolic part of eleutherococcus hancei for the preparation of a medicament for the relaxation of pre-contracted tracheal smooth muscle.

2. Use of an extract of the ethanolic part of chicken bone according to claim 1 for the preparation of a medicament for relaxing the pre-contracted tracheal smooth muscle, characterized in that: the extract of the chicken bone fragrant ethanol part achieves the application by relaxing the contraction of airway smooth muscle induced by high potassium.

3. Use of an extract of the ethanolic part of chicken bone according to claim 1 for the preparation of a medicament for relaxing the pre-contracted tracheal smooth muscle, characterized in that: the extract of the chicken bone fragrant alcohol part achieves the application by relaxing acetylcholine-induced contraction of airway smooth muscle.

4. Use of an extract of the ethanolic part of chicken bones according to any one of claims 1 to 3 in the preparation of a medicament for relaxing pre-contracted tracheal smooth muscle, characterized in that: the medicine is used for treating asthma.

5. Use of an extract of the ethanolic part of chicken bones according to any one of claims 1 to 3 in the preparation of a medicament for relaxing pre-contracted tracheal smooth muscle, characterized in that: the dosage forms of the medicine at least comprise paste, capsules, tablets and microcapsule preparations.

6. Use of an extract of the ethanolic part of chicken bones according to any one of claims 1 to 3 in the preparation of a medicament for relaxing pre-contracted tracheal smooth muscle, characterized in that: the administration modes of the medicine at least comprise injection, oral administration and subcutaneous administration.

7. Use of an extract of the ethanolic part of chicken bone according to claim 1 for the preparation of a medicament for relaxing the pre-contracted tracheal smooth muscle, characterized in that: the preparation method of the chicken bone fragrant ethanol part extract comprises the steps of crushing the root part of chicken bone fragrant, soaking in 95% ethanol solution at room temperature, and performing rotary evaporation on the filtrate obtained by filtering to obtain an ethanol extract, thus obtaining the chicken bone fragrant ethanol part extract.

Technical Field

The invention relates to the technical field of medicines, in particular to application of an extract of an ethanol part of chicken bone in preparing a medicine for relaxing pre-contracted tracheal smooth muscle.

Background

Asthma, known as bronchial asthma, is a common chronic airway inflammation. The causes of asthma are complex, associated with various factors and vary from person to person. Asthma sufferers may develop wheezing, chest tightness, cough and the like, and even dyspnea in severe cases. In recent years, the incidence of asthma is continuously increasing worldwide due to deterioration of the environment. The traditional Chinese medicine for treating asthma has a long history, and from this aspect, modern scientific and technical research is utilized to discover a reliable and effective traditional Chinese medicine for treating and relieving asthma.

The chicken bone is bitter in flavor and has anti-inflammatory and anti-tumor effects. The chemical components of the chicken bone incense comprise terpenes, volatile oil, sterols and the like, and the main component is terpenes. The chicken bone incense has been used in treating arthritis, gastritis, sore throat, cancer, etc. At present, the research report of the chicken bone incense on the aspects of treating asthma and relaxing tracheal smooth muscle is not seen.

Disclosure of Invention

The invention relates to an action mechanism of an extract of a chicken bone fragrant ethanol part in relaxing pre-contracted tracheal smooth muscle, and provides a basis for developing the extract of the chicken bone fragrant ethanol part in relaxing the tracheal smooth muscle for treating asthma.

The technical scheme provided by the invention is application of the extract of the chicken bone fragrant ethanol part in preparing a medicine for relaxing pre-contracted tracheal smooth muscle.

Wherein said extract from the ethanolic part of chicken bone achieves said use by relaxing the high potassium induced contraction of airway smooth muscle.

Wherein the extract of the chicken bone ethanol part achieves the application by relaxing acetylcholine-induced contraction of airway smooth muscle.

Furthermore, the medicament is for the treatment of asthma.

Moreover, the dosage forms of the medicine at least comprise paste, capsules, tablets and microcapsule preparations.

Furthermore, the administration modes of the drug at least include injection, oral administration and subcutaneous administration.

The preparation method of the chicken bone aroma ethanol part extract comprises the steps of crushing the root part of chicken bone aroma, soaking in 95% ethanol solution at room temperature, and performing rotary evaporation on the filtrate obtained by filtering to obtain an ethanol extract, thus obtaining the chicken bone aroma ethanol part extract.

Compared with the prior art, the technical scheme has the beneficial effects that: according to the invention, through detecting the tension of isolated tracheal ring muscle of an experimental mouse, the chicken bone aroma ethanol part extract is found to have an efficient effect of relaxing tracheal smooth muscle, and the substance is expected to be applied to relieving asthma. Provides a basis for developing the application of the extract of the chicken bone fragrant ethanol part in preparing the medicine for relaxing the pre-contracted tracheal smooth muscle.

Drawings

FIG. 1 is a graph of the vasodilation effect of chicken bone extract on pre-contracted tracheal rings induced by high potassium;

FIG. 2 shows the vasodilation effect of the extract of the ethanolic part of chicken bone on the acetylcholine-induced pre-contracted tracheal ring.

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings and examples, and the present invention is not limited to the examples.

1. Preparing the chicken bone fragrant ethanol part extract:

pulverizing 2kg of radix crotonis Crassifolii, weighing 1kg of coarse powder of radix crotonis Crassifolii, soaking in 95% ethanol solution at room temperature for 7 days at a solid-to-liquid ratio of 1: 10. Filtering to obtain filtrate, rotary evaporating the filtrate to obtain ethanol extract, and storing the extracted chicken bone aroma ethanol part in a vacuum drying oven for later use. In the experiment, the extract of the chicken bone ethanol part is dissolved in dimethyl sulfoxide (DMSO).

2. Treatment of isolated tracheal rings in mice

Male BABL/c mice at 8 weeks were sacrificed using the spinal cage and fixed ventrally up to the dissecting plate. Carefully open the chest of the mouse with scissors to expose the trachea and lung tissue of the mouse, cut the entire trachea tissue from the laryngeal node to the lung of the mouse, and place the cut tissue in PSS (NaCl 135mM, KCl 5mM, MgCl)₂ 1mM，CaCl₂2mM HEPES10mM, glucose 10mM NaOH pH 7.4). The excess tissue around the main trachea is carefully cleaned by scissors and tweezers under a stereomicroscope, and finally, the trachea with the length of about 7mm is cut in the area close to the triangle of the trachea for standby.

3. Tension detection method for mouse airway smooth muscle

The tracheal rings were secured with two triangular hooks of appropriate size, suspended to a tension transducer, placed in a thermostatted water bath containing 6mL PSS, into which oxygen was continuously introduced at a bath temperature of 37 ℃. After loading, the initial preload was adjusted to around 300mg for 15min, the PSS solution was replaced every 15min while the preload was adjusted to around 300mg, repeated 4 times. After 4 equilibria are completed, 100 μm is usedol·L^-1ACh or 80 mmol. L^-1KCl pre-stimulates the isolated tracheal ring, so that the isolated tracheal ring is fully contracted, then eluted by 6mLPSS to return to the baseline, and the isolated tracheal ring starts formal experiments after resting for 30min under the baseline state.

The experimental results are as follows:

(1) effect of the extract of the ethanolic part of chicken bone (EECC) on the relaxation of pre-contracted tracheal rings induced by high potassium

K⁺Can activate voltage-dependent calcium ion channel and promote extracellular Ca²⁺Flow into the cell to make Ca inside the cell²⁺The concentration increased, causing the tracheal rings of the mice to shrink. As shown in FIG. 1, the isolated tracheal rings of mice were surrounded by KCl (80 mmol. multidot.L)^-1) A sustained and stable shrinkage in solution occurs and a maximum shrinkage value is reached. The extract of the ethanolic part of the chicken bone can relax the contraction dose-dependently and reach the maximum relaxation value, and the maximum relaxation ratio value is (102.12 +/-0.05)%. The chicken bone ethanol extract can relax KCl-induced pre-contraction of tracheal smooth muscle, and the effect of the chicken bone ethanol extract is dose-dependent.

(2) Effect of the extract of the ethanolic part of chicken bone (EECC) on acetylcholine (ACh) -induced relaxation of pre-contracted tracheal rings

ACh can activate and regulate Ca²⁺Such as voltage-dependent calcium ion channels and non-selective cation channels. As shown in FIG. 2, ACh (100. mu. mol. L)^-1) The solution induces the isolated tracheal ring of the mouse to continuously and stably shrink and reach the maximum contraction value. The extract of the ethanolic part of the chicken bone can relax the contraction dose-dependently and reach the maximum relaxation value, and the maximum relaxation ratio value is (97.21 +/-0.39)%. The chicken bone ethanol extract is shown to relax the pre-contraction of tracheal smooth muscle induced by ACh, and the effect of the chicken bone ethanol extract is dose-dependent.

In conclusion, the experiment shows that the extract of the chicken bone fragrant ethanol part has the function of efficiently relaxing tracheal smooth muscle, and the substance is expected to be applied to relieving asthma. Provides a basis for developing the application of the extract of the chicken bone fragrant ethanol part in preparing the medicine for relaxing the pre-contracted tracheal smooth muscle.