阅读说明：本技术 二氢香豆素在抑制稗草种子萌发和生长中的应用 (Application of dihydrocoumarin in inhibiting germination and growth of barnyard grass seeds ) 是由 柏连阳 杨浩娜 王立峰 邬腊梅 李祖任 周小毛 彭琼 邓希乐 于 2021-01-05 设计创作，主要内容包括：本发明属于除草活性物质技术领域,具体涉及二氢香豆素在抑制稗草种子萌发和生长中的应用。本发明以发芽率为指标考察二氢香豆素对稗草种子萌发的影响,以种子根长和苗长/株高和株鲜重为指标考察二氢香豆素对稗草生长的影响,结果表明二氢香豆素对稗草种子萌发和生长表现出抑制作用,且随二氢香豆素浓度增加抑制作用增强,二氢香豆素可有效应用于抑制稗草种子萌发和生长,同时可应用于制备抑制稗草种子萌发和生长的产品,其对人畜毒性低,低残留,与环境相容性好。(The invention belongs to the technical field of weeding active substances, and particularly relates to application of dihydrocoumarin in inhibiting germination and growth of barnyard grass seeds. The invention inspects the influence of the dihydrocoumarin on the germination of the barnyard grass seeds by taking the germination rate as an index, inspects the influence of the dihydrocoumarin on the growth of the barnyard grass seeds by taking the root length of the seeds, the seedling length/plant height and the fresh weight of the plants as indexes, and shows that the dihydrocoumarin has an inhibiting effect on the germination and growth of the barnyard grass seeds, the inhibiting effect is enhanced along with the increase of the concentration of the dihydrocoumarin, the dihydrocoumarin can be effectively applied to inhibiting the germination and growth of the barnyard grass seeds, and can be simultaneously applied to preparing products for inhibiting the germination and growth of the barnyard grass seeds, and the dihydrocoumarin has the.)

1. Application of dihydrocoumarin in inhibiting germination and growth of barnyard grass seed is provided.

2. The use according to claim 1, wherein said dihydrocoumarin has the formula:

3. the use according to claim 1, wherein the use is the use of dihydrocoumarin in the manufacture of a product for inhibiting the germination and growth of barnyard grass seeds.

4. Use according to claim 3, wherein the concentration of dihydrocoumarin in the product for inhibiting germination and growth of barnyard grass seeds is 10-2000 mg/L.

5. Use according to claim 4, wherein the concentration of dihydrocoumarin in the product for inhibiting germination and growth of barnyard grass seeds is 50-2000 mg/L.

6. The use as claimed in claim 5, wherein the concentration of dihydrocoumarin in the product for inhibiting germination and growth of barnyard grass seeds is 500-1000 mg/L.

7. A product for inhibiting germination and growth of barnyard grass seeds comprising dihydrocoumarin as an active ingredient.

8. The product for inhibiting the germination and growth of barnyard grass seeds of claim 7, further comprising acceptable carriers and adjuvants.

9. The product for inhibiting the germination and growth of barnyard grass seeds of claim 8, wherein the acceptable carriers and adjuvants include one or more of antioxidants, chelating agents, surfactants, fillers, disintegrants, wetting agents, solvents and pH adjusting agents.

10. The product for inhibiting germination and growth of barnyard grass seeds of any one of claims 7-9, wherein the formulation of the product is powder, granule, wettable powder, soluble powder, water dispersible granule, water suspension, emulsifiable concentrate, aqueous emulsion, microemulsion, granule, aqua or tablet.

Technical Field

The invention belongs to the technical field of weeding active substances, and particularly relates to application of dihydrocoumarin in inhibiting germination and growth of barnyard grass seeds.

Background

Rice is the main food of more than 50% of the population in the world, weeds have great influence on the rice yield, and the annual loss reaches 40-60% of the total loss of the rice yield. Barnyard grass is one of the most harmful weeds in paddy fields, is usually controlled by chemical herbicides, but causes a series of problems such as drug resistance, pesticide residue, crop phytotoxicity and the like to agricultural growth along with long-term use and abuse of the chemical herbicides. On the other hand, with the increasing selection of organic foods by many people, public concern over food-related health risks has increased, which has led to a need for agricultural production to reduce dependence on chemical pesticides. The barnyard grass is often used as a receptor of various natural compounds, and natural compounds or lead compounds for preventing and controlling the barnyard grass are screened, so that green organic production of rice is promoted.

The plant natural compound has the characteristics of high efficiency, low toxicity, low residue and the like, and is often used for developing novel green herbicides and promoting green agricultural production. The dihydrocoumarin is used as a spice and is commonly used in food and cosmetics, and no literature data and related reports for preventing and controlling barnyard grass are found at present.

Disclosure of Invention

Aiming at the technical problems of drug resistance, pesticide residue, crop phytotoxicity and the like caused by applying a chemical herbicide to prevent and control barnyard grass in the prior art, the invention aims to provide a novel application of dihydrocoumarin in inhibiting the germination and growth of barnyard grass seeds, which can effectively inhibit the germination and growth of the barnyard grass seeds, and has low toxicity to human and livestock, low residue and good environmental compatibility.

In order to achieve the purpose, the invention adopts the following technical scheme:

firstly, the invention provides a new application of dihydrocoumarin in inhibiting the germination and growth of barnyard grass seeds.

The structural formula of dihydrocoumarin is shown as follows:

further, the new application is the application of the dihydrocoumarin in preparing products for inhibiting germination and growth of barnyard grass seeds.

Further, the concentration of dihydrocoumarin in the product for inhibiting germination and growth of barnyard grass seeds is 10-2000 mg/L.

Preferably, the concentration of dihydrocoumarin in the product for inhibiting germination and growth of barnyard grass seeds is 50-2000 mg/L.

More preferably, the concentration of dihydrocoumarin in the product for inhibiting the germination and growth of barnyard grass seeds is 500-1000 mg/L.

Secondly, the invention also provides a product for inhibiting the germination and growth of barnyard grass seeds, wherein the product comprises dihydrocoumarin as an active ingredient.

Furthermore, the product also comprises acceptable carriers and auxiliary materials.

Further, acceptable carriers and adjuvants include one or more of antioxidants, chelating agents, surfactants, fillers, disintegrants, wetting agents, solvents, and pH adjusting agents.

Furthermore, the product can be prepared into dosage forms of powder, granules, wettable powder, soluble powder, water dispersible granules, water suspending agents, missible oil, aqueous emulsion, microemulsion, granules, aqueous solution or tablets.

Compared with the prior art, the invention has the following beneficial effects:

the invention researches the influence of the dihydrocoumarin on the germination and growth of barnyard grass seeds by respectively adopting a culture dish and a pot culture detection method. The influence of the dihydrocoumarin on the germination of the barnyard grass seeds is examined by taking the germination rate as an index in a culture dish detection method, the influence of the dihydrocoumarin on the growth of the barnyard grass seeds is examined by taking the root length and the seedling length of the seeds as indexes, and the results show that the dihydrocoumarin has an inhibition effect on the germination and the growth of the barnyard grass seeds and is enhanced along with the increase of the concentration of the dihydrocoumarin, the inhibition rates on the germination of the barnyard grass seeds are 83.72% and 100% respectively when the concentration of the dihydrocoumarin is 500mg/L and 1000mg/L, the inhibition rates on the root length of the barnyard grass seeds are 77.73%, 96.07%, 95.2%, 100% and 100% respectively when the concentration of the dihydrocoumarin is 10mg/L, 50mg/L, 100mg/L, 500mg/L and 1000mg/L, and the inhibition rates on the seedling length of the barnyard grass seeds.

The pot culture detection method is characterized in that the influence of the dihydrocoumarin on the germination of the barnyard grass seeds is examined by taking the germination rate as an index, and the influence of the dihydrocoumarin on the growth of the barnyard grass is examined by taking the plant height and the fresh weight of the plant as indexes, and the results show that the dihydrocoumarin has the inhibition effects on the germination and the growth of the barnyard grass seeds and the inhibition effects are enhanced along with the increase of the concentration of the dihydrocoumarin, the inhibition rates of the dihydrocoumarin on the germination of the barnyard grass seeds are respectively 11.32%, 13.2%, 73.59%, 90.57% and 98.11% when the concentration of the dihydrocoumarin is 50mg/L, 100mg/L, 500mg/L, 1000mg/L and 2000mg/L, the inhibition rates of the dihydrocoumarin on the plant height of the barnyard grass are respectively 16.51%, 25.65%, 47.69%, 87.64% and 98.52%, and the inhibition rates of the.

The results show that the dihydrocoumarin provided by the invention can be effectively applied to inhibiting the germination and growth of barnyard grass seeds, can be applied to preparing products for inhibiting the germination and growth of the barnyard grass seeds, and has the advantages of low toxicity to human beings and livestock, low residue and good environmental compatibility.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The materials used in the following examples are all commercially available from conventional sources.

Example 1 Petri dish assay to determine the Effect of Dihydrocoumarin on seed Germination and growth of Echinochloa crusgalli

(1) Breaking dormancy and sterilizing seeds: treating the collected barnyard grass seeds with concentrated sulfuric acid for 4-5min, cleaning, and sequentially soaking with 3% sodium hypochlorite and 75% ethanol for 1-2 min. And (4) after sterilization, washing the mixture with clear water, and sucking surface water by using filter paper for later use.

The germination rate of the barnyard grass seeds after the treatment is higher than 85 percent.

(2) Solution preparation: the dihydrocoumarin is dissolved in a small amount of absolute ethanol and is prepared into dihydrocoumarin solutions with concentrations of 1000, 500, 100, 50, 10 and 0mg/l with pure water.

(3) Detection on a culture dish: filling 2 layers of filter paper in a 9cm disposable culture dish, adding 5ml of dihydrocoumarin solutions with different concentrations, putting 10 barnyard grass seeds with full seeds in each culture dish, placing the culture dish in a light incubator at the temperature of 26 ℃/28 ℃ for 12h/12h, measuring the germination rate, the root length and the bud length of the seeds after 5 days, and repeating the test for 3 times.

(4) As a result:

4.1 Effect of dihydrocoumarin on seed Germination of Echinochloa crusgalli:

concentration (mg/L)	Percentage of germination (%)	Inhibition ratio (%)
			0	95.56±1.11a	0
10	95.56±1.11a	0
			50	94.44±1.11a	1.17
100	94.45±2.22a	1.16
			500	15.56±1.11b	83.72
1000	0±0c	100

4.2 Effect on seed root length:

concentration (mg/L)	Root length (cm)	Inhibition ratio (%)
			0	2.29±0.13a	0
10	0.51±0.09b	77.73
			50	0.09±0.01c	96.07
100	0.11±0.02c	95.2
			500	0±0c	100
1000	0±0c	100

4.3 Effect on seed shoot Length:

concentration (mg/L)	Seedling length (cm)	Inhibition ratio (%)
			0	2.13±0.16a	0
10	1.58±0.12b	25.82
			50	1.44±0.05b	32.39
100	1.11±0.05c	47.89
			500	0.28±0.03d	86.85
1000	0±0e	100

Different letters indicate a significant difference P < 0.05 compared to 0 mg/L.

Example 2 potting assay to determine the Effect of Dihydrocoumarin on the Germination and growth of Echinochloa crusgalli seeds

(1) Breaking dormancy and sterilizing seeds: treating the collected barnyard grass seeds with concentrated sulfuric acid for 4-5min, cleaning, and sequentially soaking with 3% sodium hypochlorite and 75% ethanol for 1-2 min. And (4) after sterilization, washing the mixture with clear water, and sucking surface water by using filter paper for later use.

The germination rate of the barnyard grass seeds after the treatment is higher than 85 percent.

(2) Solution preparation: the dihydrocoumarin is dissolved in small amount of anhydrous ethanol, and prepared into dihydrocoumarin solutions with concentration of 2000, 1000, 500, 100, 50 and 0mg/l with pure water.

(3) 1/2 cups of air-dried and sieved field soil are placed into a plastic round basin with the diameter of 8.6cm and the height of 6cm, and sufficient clear water is added to completely soak the field soil for more than 2 hours. And then pouring excessive water on the soil surface in the pot, troweling the soil surface of the field, scattering 20 barnyard grass seeds with full seeds in each pot, lightly paving a layer of sieving air-dried field soil with the particle size of 0.5cm, and spraying the soil surface with a spray can.

(4) The planted barnyard grass potted plants were transferred to a 12h/12h light incubator at 26 ℃/28 ℃ and were watered gently daily so that the surface water layer of the soil was maintained at 0.5 cm. After 2 days, the surface water was decanted and 30ml of dihydrocoumarin solution was added to each pot of barnyard grass. After 10 days, the germination rate, plant height and fresh weight of the barnyard grass treated differently were observed. During the period, water was replenished every day so that the surface water layer of the soil was maintained at 0.5 cm.

(5) As a result:

5.1 Effect of dihydrocoumarin on seed Germination of Echinochloa crusgalli:

concentration (mg/L)	Percentage of germination (%)	Inhibition ratio (%)
			0	88.33±1.67a	0
50	78.33±1.67ab	11.32
			100	76.67±4.41b	13.2
500	23.33±4.41c	73.59
			1000	8.33±4.41d	90.57
2000	1.67±1.67d	98.11

5.2 Effect on barnyard grass plant height:

concentration (mg/L)	Plant height (cm)	Inhibition ratio (%)
			0	10.84±0.63a	0
50	9.05±0.49ab	16.51
			100	8.06±0.64b	25.65
500	5.67±1.05c	47.69
			1000	1.34±0.87d	87.64
2000	0.16±0.16d	98.52

5.3 Effect on fresh weight of barnyard grass on land:

concentration (mg/L)	Fresh weight of plant (mg)	Inhibition ratio (%)
			0	12.7±1.8a	0
50	12±1.2a	7.69
			100	9.7±0.9a	23.08
500	5.3±1.2b	61.54
			1000	1±0.6c	92.31
2000	0±0c	100

Different letters indicate a significant difference P < 0.05 compared to 0 mg/L.

Example 3

A product for inhibiting germination and growth of barnyard grass seeds, which comprises not only a product using dihydrocoumarin alone but also a product using dihydrocoumarin as an active ingredient. The product may also include acceptable carriers and adjuvants including one or more of antioxidants, chelating agents, surfactants, fillers, disintegrants, wetting agents, solvents and pH adjusting agents. The dosage form of the product can be powder, granules, wettable powder, soluble powder, water dispersible granules, water suspending agent, missible oil, emulsion in water, microemulsion, granules, aqueous solution or tablets. The selection of the above carriers, excipients and dosage forms is all conventional in the art.