阅读说明：本技术 一种含毒氟磷和氨基寡糖素的抗烟草病毒组合物 (Tobacco virus resisting composition containing Dufulin and amino-oligosaccharin ) 是由 罗会 王晓宇 金吉林 李兴忠 柏自琴 于 2021-01-13 设计创作，主要内容包括：本发明提供了一种含毒氟磷和氨基寡糖素的抗烟草病毒组合物。特别涉及一种毒氟磷,与氨基寡糖素复配的农药活性组合物。其中毒氟磷与氨基寡糖素复配的重量比为10∶0.3；至0.3∶10,有效成分含量为1％-90％。复配混剂加入助剂或载体后可加工成可湿性粉剂、悬浮剂、微乳剂、水乳剂、水分散粒剂、干悬乳剂及种衣剂等剂型。所述组合物用于防治由水稻条纹叶枯病毒、水稻矮缩病毒、玉米粗缩病毒、水稻黑条矮缩病毒、番茄病毒病毒、黄瓜花叶病毒、烟草花叶病毒引起的病毒病害,达到了增效、兼治、延缓抗药性、减少毒氟磷用量、降低成本等效果。(The invention provides an anti-tobacco virus composition containing Dufulin and amino-oligosaccharin. In particular to a pesticide active composition compounded by Dufulin and amino-oligosaccharin. The weight ratio of the toxic fluophosphate to the amino-oligosaccharin is 10: 0.3; to 0.3: 10, the content of effective components is 1 to 90 percent. The compound mixture can be processed into wettable powder, suspending agent, microemulsion, emulsion in water, water dispersible granule, dry suspoemulsion, seed coating agent and other dosage forms after being added with an auxiliary agent or a carrier. The composition is used for preventing and treating virus diseases caused by rice stripe virus, rice dwarf virus, corn rough dwarf virus, rice black-streaked dwarf virus, tomato virus, cucumber mosaic virus and tobacco mosaic virus, and achieves the effects of synergy, treatment, drug resistance delay, toxic fluorine and phosphorus reduction, cost reduction and the like.)

1. A pesticidal composition comprising: dufulin and amino-oligosaccharin, wherein the weight ratio of Dufulin to amino-oligosaccharin is as follows: 80: 0.3; to 0.3: 80, the content of effective components is 1 to 90 percent; wherein the Dufulin is a crystal with the following parameters: the characteristic peaks (2 theta) of the X-powder diffraction (XRD) pattern are as follows: (2 theta +/-0.2) 5.05 degrees, 8.52 degrees, 15.05 degrees, 18.05 degrees, 25.01 degrees and 25.69 degrees.

2. The pesticidal composition according to claim 1, characterized in that the composition further comprises agrochemically acceptable auxiliaries, carriers and fillers.

3. The pesticide composition as set forth in claim 1, wherein the weight ratio of Dufulin to moroxydine hydrochloride is: 70: 0.3; to 0.3: 70.

4. The pesticidal composition according to claim 1, characterized in that the weight ratio of dufulin to amino-oligosaccharin is: 60: 0.3; to 0.3: 60.

5. The pesticide composition as set forth in claim 1, wherein the weight ratio of Dufulin to Ningnanmycin is: 50: 0.3; to 0.3: 50.

6. The pesticide composition as claimed in claim 2, wherein the adjuvant is one or more of a diffusant NNO, sodium dodecyl sulfate, a diffusant MF, sodium lignosulfonate, agricultural emulsion 600#, fatty alcohol-polyoxyethylene ether, naphthalene sulfonic acid formaldehyde polycondensate, ammonium sulfate, emulsifier 603# or emulsifier 500 #; the carrier is one or more of white carbon black, kaolin, water or argil.

7. The pesticidal composition according to claims 1 to 6, characterized in that the composition is prepared as a wettable powder, a suspension concentrate, a microemulsion, an aqueous emulsion, a water dispersible granule, a dry suspoemulsion or a seed coating.

8. Pesticidal compositions according to claims 1-7, characterized in that they are used for controlling viral diseases caused by rice stripe virus, rice dwarf virus, maize rough dwarf virus, rice black-streaked dwarf virus, tomato virus, cucumber mosaic virus, tobacco mosaic virus.

9. A Dufulin compound characterized by: the Dufulin is crystal and has the following parameters: the characteristic peaks (2 theta) of the X-powder diffraction (XRD) pattern are as follows: (2 theta +/-0.2) 5.05 degrees, 8.52 degrees, 15.05 degrees, 18.05 degrees, 25.01 degrees and 25.69 degrees.

Technical Field

The invention relates to the field of pesticides, and particularly relates to a pesticide active composition of Dufulin and amino-oligosaccharin with synergistic effective dose.

Background

In recent years, large-area outbreaks of virus diseases of grain crops, economic crops, vegetables and fruits and lack of effective antiviral medicaments in China cause the virus diseases to be in disaster and the grain virus diseases to be in a world pattern trend of spreading China-southeast Asia-east Asia, so that agricultural virus and toxicity become neck bottles and obstacles influencing the normal and orderly development of agriculture in China and agriculture in the world, how to quickly and effectively obtain efficient and low-cost green antiviral pesticides becomes a focus problem of general attention of agricultural science at present. Virus diseases of rice, corn and vegetables caused by rice stripe virus, rice dwarf virus, corn rough dwarf virus, rice black-streaked dwarf virus, tomato virus, cucumber mosaic virus, tobacco mosaic virus and the like are caused to outbreak in a large area of China, which not only brings huge economic loss, but also causes serious social panic. According to the data report of national agricultural technology promotion service center, the annual occurrence area of the viral diseases of rice and corn in China reaches 5000 ten thousand mu, the average yield loss caused by the viral diseases can reach 30 percent, and the economic loss is up to 100 hundred million yuan. For example, late rice virus diseases in 2010, particularly southern rice black-streaked dwarf virus diseases, outbreak disasters in rice areas of 9 provinces such as Guangdong, Hunan and Jiangxi, and the damaged area of rice is more than 2000 mu of a thousand.

The existing chemical agents for preventing and treating viruses comprise amino-oligosaccharin, Dufulin and the like. They are characterized by: dufulin (C)₁₉H₂₂FN₂O₃PS) is a novel antiviral medicament containing fluoro amino phosphoric acid with a novel structure, and can improve the content of signal molecule salicylic acid by activating a tobacco salicylic acid signal conduction path, thereby promoting the expression of downstream disease course related protein; obtaining antiviral ability by inducing the activity of tobacco PAL, POD and SOD defense enzymes; viral invasion of the host is reduced by the aggregation of TMV particles. The medicament is environment-friendly, simple in production process, free of three-waste pollution, low in production cost compared with the commercially available medicament, and capable of improving the disease resistance of plants, so that various crystals of the medicament are expected to play a greater role in comprehensively preventing and treating plant virus diseases.

Amino-oligosaccharin (C)₆H₁₁NO₄)_n(n is more than or equal to 2) is a high-efficiency, low-toxicity, green and environment-friendly biological pesticide which can activate immune factors in plants,inducing plant to generate active disease resistance, and preventing, inhibiting and killing various fungi, bacteria and viruses. The application of the amino-oligosaccharin can not only help crops to treat and prevent diseases, but also promote the growth of the crops and enhance the drought resistance and waterlogging resistance of the crops. The amino-oligosaccharin contains rich carbon and nitrogen, can be decomposed and utilized by microorganisms and serves as a nutrient for plant growth, and is beneficial to recovery of damaged plants, promotion of root growth and seedling strengthening, enhancement of stress resistance of crops and promotion of plant growth and development.

Disclosure of Invention

The invention aims to provide an antiviral agent composition containing toxic fluorophosphate, which has stronger and more stable drug effect and lower cost, namely a pesticide active composition compounded by toxic fluorophosphate and amino-oligosaccharin.

The plant virus disease prevention and treatment medicament compounded by the Dufulin and the moroxydine hydrochloride is characterized in that the weight ratio of the Dufulin to the amino-oligosaccharin is 80: 0.3; to 0.3: 80.

The plant virus disease prevention and treatment medicament compounded by the Dufulin and the amino-oligosaccharin is characterized in that the weight ratio of the Dufulin to the amino-oligosaccharin is 70: 0.3; to 0.3: 70.

The plant virus disease prevention and treatment medicament compounded by the toxic fluophosphate and the ningnanmycin is characterized in that the weight ratio of the compounding of the toxic fluophosphate and the amino-oligosaccharin is 60: 0.3; to 0.3: 60.

The pesticide composition comprises the effective components, an auxiliary agent, a cosolvent and a filler; the auxiliary agent is one or more of a dispersing agent NNO, sodium dodecyl sulfate, a dispersing agent MF, sodium lignosulfonate, agricultural emulsion No. 600, fatty alcohol-polyoxyethylene ether, a naphthalenesulfonic acid formaldehyde condensation polymer, ammonium sulfate, an emulsifier No. 603 and an emulsifier No. 500; the cosolvent is one or more of isopropanol, dimethylformamide, xylene and ethyl acetate; the carrier is one or more of white carbon black, kaolin, water and argil.

The pesticide composition can be processed into wettable powder, suspending agent, microemulsion, aqueous emulsion, water dispersible granules, dry suspension emulsion or seed coating agent.

The composition is used for preventing and treating crop virus diseases caused by rice stripe virus, rice dwarf virus, corn rough dwarf virus, rice black-streaked dwarf virus, tomato virus, cucumber mosaic virus, tobacco mosaic virus and the like, such as rice, corn, vegetables and the like.

The invention is further illustrated by the following examples. It should be understood that the preparation methods described in the examples are only for illustrating the present invention and are not to be construed as limiting the present invention, and that the simple modifications of the preparation methods of the present invention based on the concept of the present invention are within the scope of the present invention as claimed.

Drawings

FIG. 1 is an XRD spectrum of the crystalline Dufulin of the present invention

Detailed Description

Formulation example 1

Preparation of 60% amino-oligosaccharin Dufulin wettable powder

Mixing the components together, and performing jet milling to pass through a 325-mesh sieve to obtain the product.

Formulation example 2

Preparation of 30% Dufulin-amino-oligosaccharin suspension

Mixing the above components, and grinding in sand mill until the particle diameter of effective component is less than 4 μm. The addition of sodium lignosulfonate, JFC and agricultural emulsion 600# is beneficial to the preparation of dosage forms, can keep the stability of the dosage forms, is beneficial to storage and transportation and ensures the drug effect.

Formulation example 3

Preparation of 30% amino-oligosaccharin-Dufulin microemulsion

Dissolving Dufulin and amino-oligosaccharin completely, adding emulsifier, stirring to form oil phase, adding water, and stirring to form uniform transparent homogeneous liquid.

Formulation example 4

Preparation of 30% amino-oligosaccharin Dufulin aqueous emulsion

Mixing the above components, and shearing in a shearing kettle until the particle diameter of the effective component is less than 3 μm. The emulsifier functions to form a film or double layer that charges the dispersed phase when it is dispersed on the surface of the dispersoid, preventing the droplets of the dispersed phase from coagulating. The isopropanol and the dimethylbenzene can effectively obtain the mixed missible oil and the water, and the stability of the mixed missible oil and the water is kept.

Formulation example 5

Preparation of 30% amino-oligosaccharin Dufulin water dispersible granule

And uniformly mixing the mixture, carrying out air flow crushing, adding a proper amount of water, kneading, granulating the obtained mixture, and drying to obtain the product. The addition of the auxiliary agents such as the naphthalenesulfonic acid formaldehyde polycondensate, the sodium dodecyl sulfate, the ammonium sulfate, the argil and the like not only provides guarantee for preparation formulation preparation, but also can keep good dispersibility of the product and has better adhesion to the surface of a target.

The added excipients, auxiliaries, carriers and the like are all market-sold products and are common auxiliary agents for pesticide production.

Examples of biological Activity

Tobacco mosaic disease field drug effect test:

in order to determine the control effect of the Dufulin and the amino-oligosaccharin on tobacco mosaic disease and rice virus disease when used singly or in combination, two over-year severe disease field crop test points are selected in Jinlongxiang county in 2020, and are arranged into test cells according to random cell groups, and the area per mu of the test cells is 66.7m²Spraying the test reference medicament in several growth periods of 4-5 leaves, a clumping period and a vigorous growth period from the tobacco seedling growing to the ground, repeating for 3 times and 9 cells. Full field recording at harvest.

Treatment 1: 30% Dufulin amino-oligosaccharin WP

And (3) treatment 2: 30% Dufulin crystal WP

And (3) treatment: 0.5% amino-oligosaccharin AS

And (4) treatment: 30% Dufulin WP

And (4) treatment 5: blank control

The test method comprises the following steps: the application is carried out for the first time after the tobacco is transplanted for 15-20 days, the application is carried out again at an interval of 7 days, and the application is carried out for three times.

The investigation method comprises the following steps: the results are shown in the following table after 7d, 14d and 21d of three times of drug administration, classified according to relevant data investigation standards, counted for disease index, and calculated for prevention effect.

The experimental results are as follows: as can be seen from the above table, the control effect of the drug containing Dufulin crystal is obviously better than that of the drug containing Dufulin, i.e. Dufulin crystal has higher control effect than Dufulin.