阅读说明：本技术 一种用于宠物皮肤保健的青蒿精油的制备工艺 (Preparation process of sweet wormwood essential oil for pet skin health care ) 是由 郭宇鹏 郭建钊 保广春 李英杰 张璘 于 2021-02-06 设计创作，主要内容包括：本发明提供了一种用于宠物皮肤保健的青蒿精油的制备工艺,包括以下步骤：青蒿粉碎,酶提取,过滤,水蒸气蒸馏；青蒿残渣超声提取,过滤；合并,干燥,浓缩。本发明提供的一种用于宠物皮肤保健的青蒿精油的制备工艺不仅可以提高挥发油提取率,减小挥发油损失,提高了其安全性。同时本发明制备的用于宠物皮肤保健的青蒿精油对宠物皮肤上的金色葡萄球菌有抑制作用,且效果较好。(The invention provides a preparation process of sweet wormwood essential oil for pet skin health care, which comprises the following steps: pulverizing herba Artemisiae Annuae, extracting with enzyme, filtering, and steam distilling; ultrasonically extracting sweet wormwood residue, and filtering; mixing, drying, and concentrating. The preparation process of the sweet wormwood essential oil for pet skin health care provided by the invention can improve the extraction rate of volatile oil, reduce the loss of the volatile oil and improve the safety of the volatile oil. Meanwhile, the sweet wormwood essential oil for pet skin health care, which is prepared by the invention, has an inhibiting effect on staphylococcus aureus on pet skin and has a good effect.)

1. A preparation process of sweet wormwood essential oil for pet skin health care is characterized by comprising the following steps:

s1, crushing sweet wormwood, and sieving with a 40-mesh sieve to obtain sweet wormwood powder;

s2, adding distilled water into the powder obtained in the step S1, soaking for 10-30min at 30-40 ℃, stirring, adding 0.4-1.0 wt% of biological enzyme into the sweet wormwood powder, treating for 40-60min, and filtering to obtain filtrate A and folium artemisiae argyi residues;

s3, carrying out steam distillation treatment on the filtrate A obtained in the step S2, carrying out distillation extraction for 20-40min, standing, and separating to obtain an extracting solution;

s4, adding an extracting agent which is 2-5 times of the weight of the sweet wormwood residue obtained in the step S2 into the sweet wormwood residue, soaking for 20-30min, performing ultrasonic extraction for 30-60min, and filtering to obtain a filtrate B;

s5, combining the extracting solution obtained in the step S3 with the filtrate B obtained in the step S4 to obtain a mixed solution C;

s6, drying the mixed liquor C obtained in the step S5 with anhydrous sodium sulfate, filtering, and concentrating at 60 ℃ under normal pressure to obtain the compound.

2. The preparation process of the artemisia apiacea essential oil for the pet skin health care according to the claim 1, is characterized in that: in the step S2, the biological enzyme is composed of cellulase and pectinase according to the weight ratio of 3: 1.

3. The preparation process of the artemisia apiacea essential oil for the pet skin health care according to the claim 1, is characterized in that: in the step S2, any one of sodium chloride, calcium chloride, molecular sieve and super absorbent resin is added into the essential oil as a drying agent for dehydration.

4. The preparation process of the artemisia apiacea essential oil for the pet skin health care according to the claim 1, is characterized in that: in the step S2, the wormwood powder is put into a distillation kettle, the temperature in the distillation kettle is 110-120 ℃, and the wormwood powder is distilled for 1.5-2h under the pressure value of 7-9 Mpa.

5. The preparation process of the artemisia apiacea essential oil for the pet skin health care according to the claim 1, is characterized in that: the frequency of the ultrasonic wave in the step S4 is 20-40 kHz.

6. The preparation process of the artemisia apiacea essential oil for the pet skin health care according to the claim 1, is characterized in that: in the step S4, the extracting agent consists of petroleum ether and dichloromethane at the temperature of 30-60 ℃ in a volume ratio of 2: 1.

Technical Field

The invention relates to the technical field of veterinary drugs, and in particular relates to a preparation process of sweet wormwood essential oil for pet skin health care.

Background

More and more families choose to domesticate pets, the domesticated pets are also confronted with some common pet diseases, the skin diseases of the pets become common diseases of the families for feeding the pets, and the pets are easy to breed pathogens such as bacteria, fungi, viruses, parasites and the like on the surfaces of the hair and the skin of the pets due to the characteristics of the hair and the skin of the pets, so that the pets are ill and the health of the pets and owners is affected.

Artemisia annua, a name of traditional Chinese medicine, is dry aerial parts of Artemisia annua L.of Compositae, and the Artemisia annua L.oil contains a plurality of chemical components such as artemisone, isoartemisone, eucalyptol, L-camphor, caryophyllene and pinene, and has the effects of clearing deficiency heat, removing bone steaming, relieving summer-heat, preventing malaria and removing jaundice. It is used for treating yin deficiency due to pathogenic warm, night fever and early coolness, fever due to yin deficiency, hectic fever due to yin deficiency, fever due to summer-heat pathogen, malaria with chills and fever, jaundice due to damp-heat, etc.

The main effective component of the sweet wormwood herb is volatile oil substances, and the modern pharmacological research shows that the volatile oil is beneficial to treating diseases such as yin injury caused by warm pathogen, night fever and early cooling, yin deficiency fever, bone steaming and fatigue fever, summer fever caused by pathogenic heat, malaria and jaundice caused by damp heat, and is an important raw material in the perfume industry, the food industry and the cosmetic industry.

The problem to be solved is urgently needed to produce the externally applied pet skin medicament which is suitable for pets and has small toxic and side effects by using the sweet wormwood herb into the externally applied pet skin medicament.

At present, various extraction methods of the sweet wormwood herb volatile oil comprise a steam distillation method, a solvent extraction method, a petroleum ether extraction method and the like, but the extraction rate of the volatile oil is low and the loss of the volatile oil is large in the existing extraction method.

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide a preparation process and application of sweet wormwood essential oil for pet skin health care. The preparation process of the sweet wormwood essential oil provided by the invention can improve the extraction rate of volatile oil, reduce the loss of the volatile oil and improve the safety of the volatile oil; meanwhile, the elution time of the substances with larger polarity can be effectively shortened.

In order to achieve the purpose, the invention provides the following technical scheme: a preparation process of sweet wormwood essential oil for pet skin health care comprises the following steps:

s1, crushing sweet wormwood, and sieving with a 40-mesh sieve to obtain sweet wormwood powder;

s2, adding distilled water into the powder obtained in the step S1, soaking for 10-30min at 30-40 ℃, stirring, adding 0.4-1.0 wt% of biological enzyme into the sweet wormwood powder, treating for 40-60min, and filtering to obtain filtrate A and folium artemisiae argyi residues;

s3, carrying out steam distillation treatment on the filtrate A obtained in the step S2, carrying out distillation extraction for 20-40min, standing, and separating to obtain an extracting solution;

s4, adding an extracting agent which is 2-5 times of the weight of the sweet wormwood residue obtained in the step S2 into the sweet wormwood residue, soaking for 20-30min, performing ultrasonic extraction for 30-60min, and filtering to obtain a filtrate B;

s5, combining the extracting solution obtained in the step S3 with the filtrate B obtained in the step S4 to obtain a mixed solution C;

s6, drying the mixed liquor C obtained in the step S5 with anhydrous sodium sulfate, filtering, and concentrating at 60 ℃ under normal pressure to obtain the compound.

Further, in the step S2, the biological enzyme is composed of cellulase and pectinase at a weight ratio of 3: 1.

Further, in the step S2, any one of sodium chloride, calcium chloride, molecular sieve and super absorbent resin is added into the essential oil to be dehydrated as a drying agent.

Further, the wormwood powder in the step S2 is placed into a distillation kettle, the temperature inside the distillation kettle is 110-120 ℃, and the wormwood powder is distilled for 1.5-2h under the pressure value of 7-9 Mpa.

Further, the frequency of the ultrasonic wave in the step S4 is 20-40 kHz.

Further, in the step S4, the extracting agent is composed of 30-60 ℃ petroleum ether and dichloromethane according to the volume ratio of 2: 1.

Further, the prepared sweet wormwood essential oil is applied to pet skin health care.

Compared with the prior art, the invention has the beneficial effects that:

according to the invention, the artemisia apiacea essential oil is extracted by adopting an enzyme and ultrasonic extraction method, the artemisia apiacea is fully utilized, unnecessary evaporation and waste are reduced, the extraction rate of the volatile oil can be improved, the loss of the volatile oil is reduced, and the safety of the volatile oil is improved.

Applications of an essential oil of artemisia annua for pet skin health as described herein include, but are not limited to:

1) preventing, delaying and treating mammal keratosis caused by abnormal keratinization such as acne, dandruff, desquamation pruritus, dry chap, psoriasis, scleroderma, perifollicular keratosis, etc.;

2) the cornification promoting effect softens and supplies lips, skin, hair, nails, hooves and the like of mammals, accelerates the renewal of lips, skin, hair, nails, hooves and the like of mammals, and regulates the oiliness and luster thereof;

3) the anti-pigmentation effect can promote skin to be bright, white and ruddy, and prevent, delay or treat pigmentation symptoms such as mammal skin blackening, sallow and dim, color spot, acne mark, sunburn injury, inflammation injury, etc.;

4) the anti-scarring effect prevents, delays and treats scar growth caused by irritation injury of mammalian skin inflammation, scar growth caused by external irritation injury, acne mark and other scar symptoms;

5) delaying aging, maintaining skin elasticity, improving skin elasticity and luster, preventing, delaying and treating fine lines, wrinkles, sagging, atrophy, swelling, and large pores of mammalian skin;

6) antibacterial, antifungal, and antiparasitic effects for keeping skin of mammal clean, and preventing, delaying and treating adverse conditions of mammal skin caused by bacteria, fungi, and skin parasite;

7) eliminating or relieving adverse conditions such as inflammation, swelling, pain, and hemorrhage of skin by antiinflammatory, analgesic, and hemostatic effects;

8) promoting keratinization, correcting dyskeratosis, resisting pigmentation, inhibiting scar formation, delaying skin aging, maintaining skin elasticity, resisting inflammation, relieving pain, stopping bleeding, resisting bacteria, fungi and skin parasite, and eliminating or relieving skin disorder of mammal to provide enhanced skin health and beauty.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1A process for the preparation of essential oil of Artemisia annua for skin care of pets comprises

The following steps:

s1, crushing sweet wormwood, and sieving with a 40-mesh sieve to obtain sweet wormwood powder;

s2, adding distilled water into the powder obtained in the step S1, soaking for 10min at the temperature of 30 ℃, stirring, adding biological enzyme accounting for 0.4 weight percent of the sweet wormwood herb powder, treating for 40min, and filtering to obtain filtrate A and folium artemisiae argyi residues;

s3, carrying out steam distillation treatment on the filtrate A obtained in the step S2, carrying out distillation extraction for 20min, standing, and separating to obtain an extracting solution;

s4, adding an extracting agent with the weight 2 times that of the sweet wormwood residue obtained in the step S2 into the sweet wormwood residue, soaking for 20min, performing ultrasonic extraction for 30min, and filtering to obtain a filtrate B;

s5, combining the extracting solution obtained in the step S3 with the filtrate B obtained in the step S4 to obtain a mixed solution C;

s6, drying the mixed liquor C obtained in the step S5 with anhydrous sodium sulfate, filtering, and concentrating at 60 ℃ under normal pressure to obtain the compound.

In the step S2, the biological enzyme is composed of cellulase and pectinase according to the weight ratio of 3:1, and the design can be used for comparing data measured in the two air channels respectively, so that interference is reduced.

In the step S2, any one of sodium chloride, calcium chloride, molecular sieve and super absorbent resin is added into the essential oil as a drying agent for dehydration.

And (3) putting the wormwood powder in the step S2 into a distillation still, wherein the temperature in the distillation still is 110 ℃, and the pressure value is 7Mpa for distillation for 1.5 h.

The frequency of the ultrasonic wave in the step S4 is 20 kHz.

In the step S4, the extracting agent consists of petroleum ether and dichloromethane at the temperature of 30 ℃ in a volume ratio of 2: 1.

The prepared sweet wormwood essential oil is applied to pet skin health care.

Example 2A process for preparing essential oil of Artemisia annua for skin care of pets comprises

The following steps:

s1, crushing sweet wormwood, and sieving with a 40-mesh sieve to obtain sweet wormwood powder;

s2, adding distilled water into the powder obtained in the step S1, soaking for 15min at 32.5 ℃, stirring, adding biological enzyme accounting for 0.55% of the weight of the sweet wormwood herb powder, treating for 45min, and filtering to obtain filtrate A and folium artemisiae argyi residues;

s3, carrying out steam distillation treatment on the filtrate A obtained in the step S2, carrying out distillation extraction for 25min, standing, and separating to obtain an extracting solution;

s4, adding an extracting agent with the weight being 2.75 times that of the sweet wormwood residue obtained in the step S2 into the sweet wormwood residue, soaking for 22.5min, performing ultrasonic extraction for 37.5min, and filtering to obtain a filtrate B;

s5, combining the extracting solution obtained in the step S3 with the filtrate B obtained in the step S4 to obtain a mixed solution C;

s6, drying the mixed liquor C obtained in the step S5 with anhydrous sodium sulfate, filtering, and concentrating at 60 ℃ under normal pressure to obtain the compound.

In the step S2, the biological enzyme is composed of cellulase and pectinase according to the weight ratio of 3:1, and the design can be used for comparing data measured in the two air channels respectively, so that interference is reduced.

In the step S2, any one of sodium chloride, calcium chloride, molecular sieve and super absorbent resin is added into the essential oil as a drying agent for dehydration.

In the step S2, the wormwood powder is put into a distillation still, the temperature in the distillation still is 112.5 ℃, and the wormwood powder is distilled for 1.62h under the pressure value of 7 Mpa.

The frequency of the ultrasonic wave in the step S4 is 25 kHz.

In the step S4, the extracting agent consists of petroleum ether and dichloromethane at the temperature of 37.5 ℃ in a volume ratio of 2: 1.

The prepared sweet wormwood essential oil is applied to pet skin health care.

Example 3A process for preparing essential oil of Artemisia annua for skin care of pets comprises

The following steps:

s1, crushing sweet wormwood, and sieving with a 40-mesh sieve to obtain sweet wormwood powder;

s2, adding distilled water into the powder obtained in the step S1, soaking for 20min at 35 ℃, stirring, adding biological enzyme accounting for 0.7% of the weight of the sweet wormwood herb powder, treating for 50min, and filtering to obtain filtrate A and folium artemisiae argyi residues;

s3, carrying out steam distillation treatment on the filtrate A obtained in the step S2, carrying out distillation extraction for 30min, standing, and separating to obtain an extracting solution;

s4, adding an extracting agent with the weight being 3.5 times that of the sweet wormwood residue obtained in the step S2 into the sweet wormwood residue, soaking for 25min, performing ultrasonic extraction for 45min, and filtering to obtain a filtrate B;

s5, combining the extracting solution obtained in the step S3 with the filtrate B obtained in the step S4 to obtain a mixed solution C;

s6, drying the mixed liquor C obtained in the step S5 with anhydrous sodium sulfate, filtering, and concentrating at 60 ℃ under normal pressure to obtain the compound.

In the step S2, the biological enzyme is composed of cellulase and pectinase according to the weight ratio of 3:1, and the design can be used for comparing data measured in the two air channels respectively, so that interference is reduced.

In the step S2, any one of sodium chloride, calcium chloride, molecular sieve and super absorbent resin is added into the essential oil as a drying agent for dehydration.

And (3) putting the wormwood powder in the step S2 into a distillation kettle, wherein the temperature in the distillation kettle is 115 ℃, and the wormwood powder is distilled for 1.75h under the pressure value of 7 Mpa.

The frequency of the ultrasonic wave in the step S4 is 30 kHz.

In the step S4, the extracting agent consists of petroleum ether and dichloromethane at the temperature of 45 ℃ according to the volume ratio of 2: 1.

The prepared sweet wormwood essential oil is applied to pet skin health care.

Example 4A process for the preparation of essential oil of Artemisia annua for skin care of pets comprises the steps of

The following steps:

s1, crushing sweet wormwood, and sieving with a 40-mesh sieve to obtain sweet wormwood powder;

s2, adding distilled water into the powder obtained in the step S1, soaking for 25min at 37.5 ℃, stirring, adding biological enzyme accounting for 0.85% of the weight of the sweet wormwood herb powder, treating for 55min, and filtering to obtain filtrate A and folium artemisiae argyi residues;

s3, carrying out steam distillation treatment on the filtrate A obtained in the step S2, carrying out distillation extraction for 35min, standing, and separating to obtain an extracting solution;

s4, adding an extracting agent with the weight being 4.25 times that of the sweet wormwood residue obtained in the step S2 into the sweet wormwood residue, soaking for 27.5min, performing ultrasonic extraction for 52.4min, and filtering to obtain a filtrate B;

s5, combining the extracting solution obtained in the step S3 with the filtrate B obtained in the step S4 to obtain a mixed solution C;

s6, drying the mixed liquor C obtained in the step S5 with anhydrous sodium sulfate, filtering, and concentrating at 60 ℃ under normal pressure to obtain the compound.

In the step S2, the biological enzyme is composed of cellulase and pectinase according to the weight ratio of 3:1, and the design can be used for comparing data measured in the two air channels respectively, so that interference is reduced.

In the step S2, any one of sodium chloride, calcium chloride, molecular sieve and super absorbent resin is added into the essential oil as a drying agent for dehydration.

And the wormwood powder in the step S2 is put into a distillation still, the temperature in the distillation still is 117.5 ℃, and the wormwood powder is distilled for 1.88h under the pressure value of 7 Mpa.

The frequency of the ultrasonic wave in the step S4 is 35 kHz.

In the step S4, the extracting agent consists of petroleum ether and dichloromethane at the temperature of 52.5 ℃ according to the volume ratio of 2: 1.

The prepared sweet wormwood essential oil is applied to pet skin health care.

Example 5A process for the preparation of essential oil of Artemisia annua for skin care of pets comprises the steps of

The following steps:

s1, crushing sweet wormwood, and sieving with a 40-mesh sieve to obtain sweet wormwood powder;

s2, adding distilled water into the powder obtained in the step S1, soaking for 30min at 40 ℃, stirring, adding 1.0 wt% of biological enzyme into the sweet wormwood herb powder, treating for 60min, and filtering to obtain filtrate A and folium artemisiae argyi residues;

s3, carrying out steam distillation treatment on the filtrate A obtained in the step S2, carrying out distillation extraction for 40min, standing, and separating to obtain an extracting solution;

s4, adding an extracting agent which is 5 times of the weight of the sweet wormwood residue obtained in the step S2 into the sweet wormwood residue, soaking for 30min, performing ultrasonic extraction for 60min, and filtering to obtain filtrate B;

s5, combining the extracting solution obtained in the step S3 with the filtrate B obtained in the step S4 to obtain a mixed solution C;

s6, drying the mixed liquor C obtained in the step S5 with anhydrous sodium sulfate, filtering, and concentrating at 60 ℃ under normal pressure to obtain the compound.

In the step S2, the biological enzyme is composed of cellulase and pectinase according to the weight ratio of 3:1, and the design can be used for comparing data measured in the two air channels respectively, so that interference is reduced.

In the step S2, any one of sodium chloride, calcium chloride, molecular sieve and super absorbent resin is added into the essential oil as a drying agent for dehydration.

And (3) putting the wormwood powder in the step S2 into a distillation kettle, wherein the temperature in the distillation kettle is 120 ℃, and the pressure value is 7Mpa for distillation for 2 h.

The frequency of the ultrasonic wave in the step S4 is 40 kHz.

In the step S4, the extracting agent consists of petroleum ether and dichloromethane at the temperature of 60 ℃ in a volume ratio of 2: 1.

The prepared sweet wormwood essential oil is applied to pet skin health care.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.