阅读说明：本技术 一种增强抗逆能力保水剂及制备方法 (Water-retaining agent for enhancing stress resistance and preparation method thereof ) 是由 刘祥宇 赵国正 张超 黄禹 任林林 于 2020-12-30 设计创作，主要内容包括：本发明涉及一种增强抗逆能力保水剂,由以下组分制备而成：丙烯酸和丙烯酰胺、负载6%的2,4-表芸苔素内酯的介孔碳纳米球、过硫酸钾、N,N－亚甲基双丙烯酰胺、氢氧化钾、魔芋葡甘聚糖和大豆多糖、去离子水；还公开了其制备方法。本发明达到的有益效果是：保水剂具有良好的吸水能力,同时通过对2,4-表芸苔素内酯良好的释放控制,进一步增强了植物的抗旱抗逆能力。(The invention relates to a water-retaining agent for enhancing stress resistance, which is prepared from the following components: acrylic acid and acrylamide, mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide, potassium persulfate, N-methylene bisacrylamide, potassium hydroxide, konjac glucomannan and soybean polysaccharide and deionized water; its preparing process is also disclosed. The invention achieves the following beneficial effects: the water-retaining agent has good water absorption capacity, and simultaneously, the drought resistance and stress resistance of plants are further enhanced by good release control of 2, 4-epibrassinolide.)

1. The water-retaining agent for enhancing stress resistance is characterized by comprising the following components in parts by weight: the preparation is prepared from the following components:

acrylic acid, acrylamide, konjac glucomannan and soybean polysaccharide which are used as basic water-retaining materials;

mesoporous carbon nanospheres loaded with 2, 4-epibrassinolide;

potassium hydroxide;

an initiator;

a crosslinking agent;

deionized water.

2. The water-retaining agent for enhancing stress resistance and the preparation method thereof according to claim 1, wherein the water-retaining agent comprises the following components: the initiator is potassium persulfate, and the cross-linking agent is N, N-methylene bisacrylamide.

3. The water-retaining agent for enhancing stress tolerance as claimed in claim 2, wherein: in the mesoporous carbon nanosphere loaded with the 2, 4-epibrassinolide, 6 percent of the 2, 4-epibrassinolide is loaded on the mesoporous carbon nanosphere in percentage by weight.

4. The water-retaining agent for enhancing stress tolerance as claimed in claim 3, wherein: the water-retaining agent comprises, by weight, 15-30 parts of potassium hydroxide, 30-60 parts of acrylic acid, 10-50 parts of acrylamide, 5-15 parts of konjac glucomannan, 5-15 parts of soybean polysaccharide, 0.1-1 part of potassium persulfate, 0.05-0.2 part of N, N-methylene bisacrylamide, 0.01-0.05 part of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide and 300 parts of deionized water.

5. A preparation method of the water-retaining agent for enhancing stress resistance of claim 3 or 4 is characterized in that: the method comprises the following steps:

s1, dissolving potassium hydroxide in part of deionized water to prepare a potassium hydroxide solution;

s2, dissolving acrylic acid in the rest deionized water, and neutralizing the acrylic acid solution with a potassium hydroxide solution;

s3, adding acrylamide, konjac glucomannan and soybean polysaccharide, after the materials are completely dissolved, sequentially adding potassium persulfate and N, N-methylene bisacrylamide, heating to 65 ℃, and reacting for at least 3H;

s4, putting the mesoporous carbon nanospheres loaded with the 2, 4-epibrassinolide;

s5, after being mixed uniformly, discharging and sending into a vacuum drying chamber, and carrying out vacuum drying at 40 ℃;

and S6, after drying, crushing by using a crusher to obtain a finished product.

6. The water-retaining agent for enhancing stress resistance and the preparation method thereof according to claim 5, wherein the water-retaining agent comprises the following components: the mesoporous carbon nanosphere loaded with the 2, 4-epibrassinolide is prepared by adopting the following method: firstly, 1 part by mass of mesoporous carbon nanospheres is put into 80 parts by mass of 2mg/mL 2, 4-epibrassinolide solution, and stirred for 24 hours; centrifuging, filtering, separating precipitate, washing with phosphate buffer solution, and vacuum drying at 30 deg.C to obtain the final product.

Technical Field

The invention relates to the technical field of water-retaining agents, in particular to a water-retaining agent for enhancing stress resistance and a preparation method thereof.

Background

The water-retaining agent resin is a high molecular material which can absorb and store hundreds or even thousands of times of water by swelling, and can be used as a micro reservoir in soil. When the soil is dry, the water-retaining agent can release the water stored in the water-retaining agent for the crops to absorb, so that the crops can survive under the dry condition. However, the traditional water-retaining agent has a limited effect on guiding the drought resistance of crops.

Brassinolide is a kind of plant growth regulator, and can be used for field crops, fruits, vegetables, flowers and the like. Has multiple functions of maintaining and coordinating nutrition balance, resisting drought and cold, enhancing crop stress resistance and the like. Therefore, some mix brassinolide and water-retaining agent resin powder to improve the drought-resistant and stress-resistant effects of crops. However, the mode is not ideal for controlling the release of brassinolide, so that the final drought-resistant and stress-resistant effects are influenced.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide the water-retaining agent with the enhanced anti-stress capability, which has good water absorption capability and can well control the release of 2, 4-epibrassinolide, and the preparation method thereof.

The purpose of the invention is realized by the following technical scheme: a water-retaining agent for enhancing stress resistance is prepared from the following components:

acrylic acid, acrylamide, konjac glucomannan and soybean polysaccharide which are used as basic water-retaining materials; mesoporous carbon nanospheres loaded with 2, 4-epibrassinolide; potassium hydroxide; an initiator; a crosslinking agent; deionized water.

Further, the initiator is potassium persulfate, and the crosslinking agent is N, N-methylene-bisacrylamide.

Preferably, in the mesoporous carbon nanosphere loaded with 2, 4-epibrassinolide, 6% of 2, 4-epibrassinolide is loaded in the mesoporous carbon nanosphere in percentage by weight.

Further, in the water-retaining agent, by weight, 15-30 parts of potassium hydroxide, 30-60 parts of acrylic acid, 10-50 parts of acrylamide, 5-15 parts of konjac glucomannan, 5-15 parts of soybean polysaccharide, 0.1-1 part of potassium persulfate, 0.05-0.2 part of N, N-methylene bisacrylamide, 0.01-0.05 part of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide and 300 parts of deionized water.

A preparation method of a water-retaining agent for enhancing stress resistance comprises the following steps:

s1, dissolving potassium hydroxide in part of deionized water to prepare a potassium hydroxide solution;

s2, dissolving acrylic acid in the rest deionized water, and neutralizing the acrylic acid solution with a potassium hydroxide solution;

s3, adding acrylamide, konjac glucomannan and soybean polysaccharide, after the materials are completely dissolved, sequentially adding potassium persulfate and N, N-methylene bisacrylamide, heating to 65 ℃, and reacting for at least 3H;

s4, putting the mesoporous carbon nanospheres loaded with the 2, 4-epibrassinolide;

s5, after being mixed uniformly, discharging and sending into a vacuum drying chamber, and carrying out vacuum drying at 40 ℃;

and S6, after drying, crushing by using a crusher to obtain a finished product.

Further, the mesoporous carbon nanosphere loaded with the 2, 4-epibrassinolide is prepared by the following steps: firstly, 1 part by mass of mesoporous carbon nanospheres is put into 80 parts by mass of 2mg/mL 2, 4-epibrassinolide solution, and stirred for 24 hours; and centrifuging, filtering, separating and precipitating, washing by using a phosphate buffer solution, and drying at 30 ℃ in vacuum to obtain a finished product.

The 2, 4-epibrassinolide used in the preparation of the multifunctional water-retaining agent has good effect on relieving various adversity stresses, and after the multifunctional water-retaining agent is applied to plants, the activity of enzymes such as CAT, POD, SOD and PPO in the plants can be improved to relieve the damage to the plants caused by drought, low temperature or high temperature. The mesoporous carbon nanospheres have the advantages of high surface area, large pore volume, large pore diameter and the like, so that the mesoporous carbon nanospheres become an effective drug carrier material. It can load various drugs in the mesoporous pore canal or on the surface of the mesoporous, and can slowly release the drug-loaded drugs.

In the preparation process of the water retention agent for enhancing the stress resistance of plants, konjac glucomannan and soybean polysaccharide are introduced into the water retention agent structure in a free radical polymerization mode (the konjac glucomannan and the soybean polysaccharide are grafted onto acrylic acid and acrylamide chains through free radical polymerization in a polymerization reaction to form a part of the chemical structure of the water retention resin), so that the water absorption property and the biodegradability are improved; the mesoporous carbon nanospheres loaded with the 2, 4-epibrassinolide are added at the later stage of the polymerization reaction, and then the mesoporous carbon nanospheres are dried and crushed to seal the mesoporous carbon nanospheres loaded with the 2, 4-epibrassinolide in the water-retaining agent resin. When the water-retaining agent gel is applied into soil, the water-retaining agent provides water for plants, and meanwhile, the 2, 4-epibrassinolide in the mesoporous carbon nanospheres in the gel is slowly released, so that the resistance of the plants to drought, low temperature or high temperature is enhanced.

The invention has the following advantages:

(1) the soybean polysaccharide is introduced into the structure of the water retaining agent, so that the water absorption is improved, and the 2, 4-epibrassinolide release control is facilitated through the synergistic degradation of the konjac glucomannan.

(2) At the later stage of polymerization reaction, the mesoporous carbon nanospheres loaded with the 2, 4-epibrassinolide are added, and then the mesoporous carbon nanospheres are dried and crushed to seal the mesoporous carbon nanospheres loaded with the 2, 4-epibrassinolide in the water-retaining agent resin, so that the release control of the 2, 4-epibrassinolide is enhanced, and the mesoporous carbon nanospheres are cooperated with the characteristics of the water-retaining agent resin, and the drought resistance and stress resistance of crops are finally improved.

Detailed Description

The present invention is further described below, but the scope of the present invention is not limited to the following.

Example 1

A water-retaining agent for enhancing stress resistance is prepared from the following raw materials: 15g of potassium hydroxide, 30g of acrylic acid, 50g of acrylamide, 5g of konjac glucomannan, 9g of soybean polysaccharide, 0.5g of potassium persulfate, 0.15g of N, N-methylene bisacrylamide, 0.01g of mesoporous carbon nanospheres loaded with 6% 2, 4-epibrassinolide and 300g of deionized water.

In this embodiment, the preparation method of the water-retaining agent for enhancing the stress resistance of plants comprises the following steps: first, 15g of potassium hydroxide was dissolved in 100g of deionized water, and then 30g of acrylic acid was dissolved in 200g of deionized water. Then, the acrylic acid solution is neutralized by using a potassium hydroxide solution, and 50g of acrylamide, 5g of konjac glucomannan and 9g of soybean polysaccharide are added. After the dissolution is completed, sequentially adding 0.5g of potassium persulfate and 0.15g of N, N-methylene-bisacrylamide; heating to 65 ℃, reacting for at least three hours, and adding 0.01g of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide. After being mixed evenly, the mixture is discharged and sent into a vacuum drying chamber for vacuum drying at 40 ℃; and after the drying is finished, crushing by using a crusher to obtain a finished product.

Example 2:

the water-retaining agent for enhancing the stress resistance of plants in the embodiment 2 is prepared from the following raw materials: 25g of potassium hydroxide, 50g of acrylic acid, 25g of acrylamide, 15g of konjac glucomannan, 5g of soybean polysaccharide, 1g of potassium persulfate, 0.2g of N, N-methylene bisacrylamide, 0.04g of mesoporous carbon nanospheres loaded with 6% 2, 4-epibrassinolide and 300g of deionized water.

The preparation process of the water-retaining agent for enhancing the stress resistance of plants in the embodiment 2 is as follows: 25g of potassium hydroxide was first dissolved in 100g of deionized water, and 50g of acrylic acid was then dissolved in 200g of deionized water. Then, the acrylic acid solution is neutralized by using a potassium hydroxide solution, and 25g of acrylamide, 15g of konjac glucomannan and 5g of soybean polysaccharide are added. After the dissolution is completed, 1g of potassium persulfate and 0.2g of N, N-methylene-bisacrylamide are added in sequence; heating to 65 ℃, reacting for at least three hours, and adding 0.04g of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide. After being mixed evenly, the mixture is discharged and sent into a vacuum drying chamber for vacuum drying at 40 ℃; and after the drying is finished, crushing by using a crusher to obtain a finished product.

Example 3:

the water-retaining agent for enhancing the stress resistance of plants in the embodiment 3 is prepared from the following raw materials: 30g of potassium hydroxide, 60g of acrylic acid, 17g of acrylamide, 5g of konjac glucomannan, 15g of soybean polysaccharide, 0.1g of potassium persulfate, 0.05g of N, N-methylene bisacrylamide, 0.013g of mesoporous carbon nanospheres loaded with 6% 2, 4-epibrassinolide and 300g of deionized water.

The preparation process of the water-retaining agent for enhancing the stress resistance of plants in the embodiment 3 is as follows: 30g of potassium hydroxide was first dissolved in 100g of deionized water, and then 60g of acrylic acid was dissolved in 200g of deionized water. Then, the acrylic acid solution is neutralized by using a potassium hydroxide solution, and then 17g of acrylamide, 5g of konjac glucomannan and 15g of soybean polysaccharide are added. After the dissolution is completed, sequentially adding 0.1g of potassium persulfate and 0.05g of N, N-methylene-bisacrylamide; heating to 65 ℃, reacting for at least three hours, and adding 0.013g of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide. After being mixed evenly, the mixture is discharged and sent into a vacuum drying chamber for vacuum drying at 40 ℃; and after the drying is finished, crushing by using a crusher to obtain a finished product.

Example 4:

the water-retaining agent for enhancing plant stress resistance of the embodiment 4 is prepared from the following raw materials: 15g of potassium hydroxide, 30g of acrylic acid, 42g of acrylamide, 5g of konjac glucomannan, 15g of soybean polysaccharide, 0.5g of potassium persulfate, 0.15g of N, N-methylene bisacrylamide, 0.03g of mesoporous carbon nanospheres loaded with 6% 2, 4-epibrassinolide and 300g of deionized water.

The preparation process of the water-retaining agent for enhancing the stress resistance of plants in the embodiment 4 is as follows: first, 15g of potassium hydroxide was dissolved in 100g of deionized water, and then 30g of acrylic acid was dissolved in 200g of deionized water. Then, the acrylic acid solution is neutralized by using a potassium hydroxide solution, and then 42g of acrylamide, 5g of konjac glucomannan and 15g of soybean polysaccharide are added. After the dissolution is completed, sequentially adding 0.5 parts of potassium persulfate and 0.15 parts of N, N-methylene-bisacrylamide; heating to 65 ℃, reacting for at least three hours, and adding 0.03g of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide. After being mixed evenly, the mixture is discharged and sent into a vacuum drying chamber for vacuum drying at 40 ℃; and after the drying is finished, crushing by using a crusher to obtain a finished product.

Example 5:

the water-retaining agent for enhancing plant stress resistance of the embodiment 5 is prepared from the following raw materials: 20g of potassium hydroxide, 40g of acrylic acid, 40g of acrylamide, 7g of konjac glucomannan, 5g of soybean polysaccharide, 0.6g of potassium persulfate, 0.05g of N, N-methylene bisacrylamide, 0.03g of mesoporous carbon nanospheres loaded with 6% 2, 4-epibrassinolide and 300g of deionized water.

The preparation process of the water-retaining agent for enhancing the stress resistance of plants in the embodiment 5 is as follows: first 20g of potassium hydroxide was dissolved in 100g of deionized water, and then 40g of acrylic acid was dissolved in 200g of deionized water. Then, the acrylic acid solution is neutralized by using a potassium hydroxide solution, and then, 40g of acrylamide, 7g of konjac glucomannan and 5g of soybean polysaccharide are added. After the dissolution is completed, 0.6g of potassium persulfate and 0.05g of N, N-methylene-bisacrylamide are added in sequence; heating to 65 ℃, reacting for at least three hours, and adding 0.03g of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide. After being mixed evenly, the mixture is discharged and sent into a vacuum drying chamber for vacuum drying at 40 ℃; and after the drying is finished, crushing by using a crusher to obtain a finished product.

Example 6:

the water-retaining agent for enhancing plant stress resistance of the embodiment 6 is prepared from the following raw materials: 30g of potassium hydroxide, 60g of acrylic acid, 15g of acrylamide, 10g of konjac glucomannan, 10g of soybean polysaccharide, 0.1g of potassium persulfate, 0.05g of N, N-methylene bisacrylamide, 0.025g of mesoporous carbon nanospheres loaded with 6% 2, 4-epibrassinolide and 300g of deionized water.

The preparation process of the water-retaining agent for enhancing the stress resistance of plants in the embodiment 6 is as follows: 30g of potassium hydroxide was first dissolved in 100g of deionized water, and then 60g of acrylic acid was dissolved in 200g of deionized water. Then neutralizing the acrylic acid solution by using a potassium hydroxide solution, and then adding 15g of acrylamide, 10g of konjac glucomannan and 10g of soybean polysaccharide. After the dissolution is completed, sequentially adding 0.1g of potassium persulfate and 0.05g of N, N-methylene-bisacrylamide; heating to 65 ℃, reacting for at least three hours, and then adding 0.025g of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide. After being mixed evenly, the mixture is discharged and sent into a vacuum drying chamber for vacuum drying at 40 ℃; and after the drying is finished, crushing by using a crusher to obtain a finished product.

Example 7:

the water-retaining agent for enhancing plant stress resistance of the embodiment 7 is prepared from the following raw materials: 30g of potassium hydroxide, 60g of acrylic acid, 17g of acrylamide, 15g of konjac glucomannan, 5g of soybean polysaccharide, 0.1g of potassium persulfate, 0.05g of N, N-methylene bisacrylamide, 0.013g of mesoporous carbon nanospheres loaded with 6% 2, 4-epibrassinolide and 300g of deionized water.

The preparation process of the water-retaining agent for enhancing the stress resistance of plants in the embodiment 7 is as follows: 30g of potassium hydroxide was first dissolved in 100g of deionized water, and then 60g of acrylic acid was dissolved in 200g of deionized water. Then, the acrylic acid solution is neutralized by using a potassium hydroxide solution, and then 17g of acrylamide, 15g of konjac glucomannan and 5g of soybean polysaccharide are added. After the dissolution is completed, sequentially adding 0.1g of potassium persulfate and 0.05g of N, N-methylene-bisacrylamide; heating to 65 ℃, reacting for at least three hours, and adding 0.013g of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide. After being mixed evenly, the mixture is discharged and sent into a vacuum drying chamber for vacuum drying at 40 ℃; and after the drying is finished, crushing by using a crusher to obtain a finished product.

Example 8:

the water-retaining agent for enhancing plant stress resistance of the embodiment 8 is prepared from the following raw materials: 26g of potassium hydroxide, 52g of acrylic acid, 20g of acrylamide, 15g of konjac glucomannan, 5g of soybean polysaccharide, 0.5g of potassium persulfate, 0.1g of N, N-methylene bisacrylamide, 0.038g of mesoporous carbon nanospheres loaded with 6% 2, 4-epibrassinolide and 300g of deionized water.

The preparation process of the water-retaining agent for enhancing the stress resistance of plants in the embodiment 8 is as follows: 26g of potassium hydroxide was first dissolved in 100g of deionized water, and 52g of acrylic acid was then dissolved in 200g of deionized water. Then, the acrylic acid solution is neutralized by using a potassium hydroxide solution, and then 20g of acrylamide, 15g of konjac glucomannan and 5g of soybean polysaccharide are added. After the dissolution is completed, 0.5g of potassium persulfate and 0.1g of N, N-methylene-bisacrylamide are added in sequence; heating to 65 ℃, reacting for at least three hours, and adding 0.038g of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide. After being mixed evenly, the mixture is discharged and sent into a vacuum drying chamber for vacuum drying at 40 ℃; and after the drying is finished, crushing by using a crusher to obtain a finished product.

Example 9:

the water-retaining agent for enhancing plant stress resistance of the embodiment 9 is prepared from the following raw materials: 30g of potassium hydroxide, 60g of acrylic acid, 20g of acrylamide, 7g of konjac glucomannan, 5g of soybean polysaccharide, 1g of potassium persulfate, 0.1g of N, N-methylene bisacrylamide, 0.01g of mesoporous carbon nanospheres loaded with 6% 2, 4-epibrassinolide and 300g of deionized water.

The preparation process of the water-retaining agent for enhancing the stress resistance of plants in the embodiment 9 is as follows: 30g of potassium hydroxide was first dissolved in 100g of deionized water, and then 60g of acrylic acid was dissolved in 200g of deionized water. Then, the acrylic acid solution is neutralized by using a potassium hydroxide solution, and then 20g of acrylamide, 7g of konjac glucomannan and 5g of soybean polysaccharide are added. After the dissolution is completed, sequentially adding 1g of potassium persulfate and 0.1g of N, N-methylene-bisacrylamide; heating to 65 ℃, reacting for at least three hours, and adding 0.01g of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide. After being mixed evenly, the mixture is discharged and sent into a vacuum drying chamber for vacuum drying at 40 ℃; and after the drying is finished, crushing by using a crusher to obtain a finished product.

Example 10:

the water-retaining agent for enhancing plant stress resistance of the embodiment 10 is prepared from the following raw materials: 30g of potassium hydroxide, 60g of acrylic acid, 10g of acrylamide, 10g of konjac glucomannan, 10g of soybean polysaccharide, 0.1g of potassium persulfate, 0.05g of N, N-methylene bisacrylamide, 0.05g of mesoporous carbon nanospheres loaded with 6% 2, 4-epibrassinolide and 300g of deionized water.

The preparation process of the water-retaining agent for enhancing stress resistance of plants in this embodiment 10 is as follows: 30g of potassium hydroxide was first dissolved in 100g of deionized water, and then 60g of acrylic acid was dissolved in 200g of deionized water. Then neutralizing the acrylic acid solution by using a potassium hydroxide solution, and then adding 10g of acrylamide, 10g of konjac glucomannan and 10g of soybean polysaccharide. After the dissolution is completed, sequentially adding 0.1g of potassium persulfate and 0.05g of N, N-methylene-bisacrylamide; heating to 65 ℃, reacting for at least three hours, and adding 0.05g of mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide. After being mixed evenly, the mixture is discharged and sent into a vacuum drying chamber for vacuum drying at 40 ℃; and after the drying is finished, crushing by using a crusher to obtain a finished product.

Test example 1

A polyacrylic acid-acrylamide-konjac glucomannan type water-retaining agent was used as a control, and a water absorption multiple test was performed with the water-retaining agents obtained by the components and preparation methods in the above examples 3 and 7.

Adopting a mesh bag method: firstly, 1.00g of water-retaining agent is put into excessive standard hard water and is swelled for half an hour; then transferring the hydrogel into gauze for filtering until no water drops, and then transferring the hydrogel of the water-retaining agent into a beaker for weighing; the test was repeated three times and averaged, and the test results are shown in table 1: the comparison shows that the water absorption times of the water retention agent can be effectively increased by introducing the soybean polysaccharide into the water retention agent.

TABLE 1 Water absorption Capacity of Water-retaining Agents

Name (R)	Example 3	Example 7	Control sample
				Multiple of water absorption	466	442	425

Test example 2

The water-retaining agent obtained by the components and the preparation method in the embodiment 3 is used for drought resistance and stress resistance tests.

Firstly, uniformly mixing sand and sandy loam according to the ratio of 1:2 to obtain soil for testing. The soil for the test was then placed in pots, 2kg of soil per pot. Meanwhile, selecting red-color strawberry plants with good and consistent growth vigor as test plants.

1g of example 3 was taken up 300g of clear water and the absorbed gel was placed in a pot of soil:

then transplanting a red strawberry plant to a flowerpot as a test sample A;

transplanting a red strawberry plant into a flowerpot, and pouring clear water with the same amount as the test sample A to serve as a blank sample;

putting a red strawberry plant into a flowerpot, and pouring clear water and mesoporous carbon nanospheres which are equal to the test sample A into the flowerpot as a control sample 1;

a red strawberry plant was placed in a flowerpot, and the same amount of clear water and 2, 4-epibrassinolide as that of test sample A was poured as control sample 2.

1g of polyacrylic acid-acrylamide-konjac glucomannan-soybean polysaccharide water-retaining agent absorbs 300g of clear water, the gel after water absorption is placed in a pot of soil, and then a red strawberry plant is transplanted to a flowerpot as a control 3.

Mixing the mesoporous carbon nanospheres and the polyacrylic acid-acrylamide-konjac glucomannan-soybean polysaccharide water-retaining agent in equal proportion to the test sample A, taking 1g of the mixed solution to absorb 300g of clear water, placing the absorbed gel in a pot of soil, and then transplanting a red strawberry plant to a flowerpot as a control sample 4.

1g of polyacrylic acid-acrylamide-konjac glucomannan-soybean polysaccharide water-retaining agent was allowed to absorb 300g of clear water containing 2, 4-epibrassinolide in an amount equivalent to that of test sample A, the absorbed gel was placed in soil in a pot, and a red strawberry plant was transplanted into a flowerpot as control sample 5.

A red strawberry plant is poured into a flowerpot, and clear water and mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide which are equal to the test sample A are poured into the flowerpot to serve as a control sample 6.

Mixing mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide and a polyacrylic acid-acrylamide-konjac glucomannan-soybean polysaccharide water-retaining agent in equal proportion to a test sample A, taking 1g of the mixture to absorb 300g of clear water, placing the absorbed gel in a pot of soil, and then transplanting a red strawberry plant to a flowerpot as a control sample 7.

And selecting three rain sheltering test points. After each test point, 20 pots of each sample are placed for a fixed time, the survival rate of the red strawberry plants is used as the evaluation standard of the drought resistance and stress resistance, and the results are shown in table 2.

TABLE 2 Red strawberry plant survival rate

Sample (I)	Set for 21d
		Assay A survival/%)	78.3
Blank survival/%)	3.3
		Control 1 survival/%)	1.7
Control 2 survival/The	18.3
		Control 3 survival%	41.7
Control 4 survival%	43.3
		Control 5 survival/%)	56.6
Control 6 survival%	26.6
		Control 7 survival%	65.0

It should be noted that: survival rate is 100% of surviving strains per total strains, and the survival rates of the three test points are averaged. The test environment temperature is 22-31 ℃, and the relative humidity is 40-70%.

As can be seen from table 2:

(1) it can be concluded from comparison between control 1 and control 2 that the mesoporous carbon nanoball itself does not improve the survival rate.

(2) By adding 2, 4-epibrassinolide to control 2, it can be seen that the survival rate is improved from 1.7 to 18.3 by adding 2, 4-epibrassinolide compared with control 1, and the survival rate can be improved to a certain extent.

As can be seen by comparing control 3, control 4 and control 5, the survival rates of 41.7% and 43.3% were increased to 56.6% by adding 2, 4-epibrassinolide.

Therefore, the survival rate can be effectively improved after the 2, 4-epibrassinolide is added.

(3) Compared with the control sample 2 and the control sample 6, the survival rate of the mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide is higher after the 2, 4-epibrassinolide is directly added compared with the mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide.

Therefore, the mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide can be obtained, and the 2, 4-epibrassinolide is slowly released, so that the stress resistance of plants can be improved.

(4) It can be seen from the comparison sample 6 and the comparison sample 7 that by simply mixing the mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide with the polyacrylic acid-acrylamide-konjac glucomannan-soybean polysaccharide type water-retaining agent, the stress resistance of the plants is better improved than that of the mesoporous carbon nanospheres loaded with 6% of 2, 4-epibrassinolide alone.

Finally, the comparison between the reference sample 7 and the test sample A shows that the mesoporous carbon nanospheres loaded with the 2, 4-epibrassinolide are sealed in the water-retaining agent resin, so that the release control of the 2, 4-epibrassinolide is enhanced, and the mesoporous carbon nanospheres are cooperated with the characteristics of the water-retaining agent resin to further improve the drought resistance and stress resistance of crops.

The above embodiments are further illustrative of the present invention, but it should not be construed that the scope of the above subject matter is limited to the above embodiments. All the technologies realized based on the above contents belong to the scope of the present invention.