阅读说明：本技术 脑源肽或融合肽在新生儿缺氧缺血性脑损伤药物中的应用 (Application of brain-derived peptide or fusion peptide in medicine for treating neonatal hypoxia ischemic brain injury ) 是由 邱洁 侯雪雯 袁子钧 于 2020-06-17 设计创作，主要内容包括：本发明公开了脑源肽HIBDAP或所述的融合肽TAT-HIBDAP在制备预防或治疗新生儿缺氧缺血性脑损伤药物中的应用。脑源肽的氨基酸序列为HSQFIGYPITLFVEKER。本发明的多肽及其多肽类药物具有高选择性、高效性、耐受性好的优点,而相较于蛋白质药物,其生产更简单、成本更低。(The invention discloses an application of brain-derived peptide HIBDAP or fusion peptide TAT-HIBDAP in preparing a medicament for preventing or treating neonatal hypoxic-ischemic brain injury. The amino acid sequence of the brain-derived peptide is HSQFIGYPITLFVEKER. The polypeptide and the polypeptide drug thereof have the advantages of high selectivity, high efficiency and good tolerance, and compared with protein drugs, the polypeptide and the polypeptide drug thereof are simpler to produce and lower in cost.)

1. The application of brain-derived peptide HIBDAP or fusion peptide TAT-HIBDAP in preparing a medicament for preventing or treating neonatal hypoxic-ischemic brain injury.

2. The use of claim 1, wherein the brain-derived peptide has an amino acid sequence of HSQFIGYPITLFVEKER.

3. The use according to claim 1, wherein the fusion peptide TAT-HIBDAP consists of a cell-penetrating peptide and the brain-derived peptide HIBDAP according to claim 1, and the amino acid sequence of the fusion peptide TAT-HIBDAP is RKKRRQRRRAHSQFIGYPITLFVEKER.

4. The use according to claim 1, wherein the use is carried out by examining the effect of the fusion peptide TAT-HIBDAP on the expression of NLRP3, ASC and/or Caspase-1.

5. The use according to claim 1, wherein the detection method employs real-time quantitative PCR and/or immunoblot hybridization methods.

6. The use according to claim 5, wherein the sequences of the primer pairs used in the real-time quantitative PCR are respectively:

NLRP3：

F：5’-TGA AGA GTG TGA TCT GCG GAA AC-3’；

R：5’-GAA AGT CAT GTG GCT GAA GCT GT-3’；

ASC：

F：5’-AGT TGA TGG TTT GCT GGA TGC T-3’；

R：5’-GGT CTG TCA CCA AGT AGG GCT G-3’；

Caspase-1：

F：5’-AAC CTT GGG CTT GTC TTT-3’；

R：5’-CAG GAG GGA ATA TGT GGG-3’；

GAPDH：

F：5’-AGA AGG CTG GGG CTC ATT TG-3’；

R：5’-AGG GGC CAT CCA CAG TCT TC-3’。

7. the use according to claim 5, wherein the antibody used in the immunoblot hybridization method is NLRP3, ASC, Caspase-1 or β -actin, respectively.

Technical Field

The invention belongs to the technical field of biological medicines, and particularly relates to a brain-derived peptide and application thereof.

Background

Neonatal Hypoxic ischemic brain injury (HIBD) is one of the most common causes of neonatal death, and permanent brain damage such as sequelae of cerebral palsy, epilepsy, mental retardation, etc. may remain. However, to date, there is a lack of specific therapies for neonatal HIBD, other than sub-hypothermic therapy, and the occurrence of neurological sequelae cannot be completely prevented. Therefore, finding a way to effectively intervene on the HIBD of the newborn is urgent.

In recent years, polypeptide drugs have been widely used for the treatment of metabolic diseases, tumors, cardiovascular diseases, and nervous system diseases, because of their many advantages.

The natural polypeptide has the defects of poor chemical and physical stability and short half-life, and with the development of a series of new technologies, such as reducing the degradation of the polypeptide by replacing specific amino acid, prolonging the half-life of the polypeptide by combining with albumin, optimizing the pharmacokinetics of the polypeptide by chemical modification and the like, the inherent defects of the natural polypeptide can be overcome, so that the polypeptide medicine is easier to popularize in a large range in clinic. So far, more than 60 polypeptide drugs are put on the market for patients, more than 150 polypeptides are in clinical development stage, and the polypeptide drugs have great potential as a new treatment approach.

Disclosure of Invention

The purpose of the invention is as follows: the invention discovers that a polypeptide derived from 210-226 amino acids of heat shock protein 90 alpha (Hsp90 alpha) is remarkably reduced in cerebrospinal fluid of a child suffering from HIBD through a polypeptinomics screening technology. Under hypoxic conditions, HSP90 α plays an important role in the activation of HIF-1 α in conjunction with hypoxia inducible factor 1 α (HIF-1 α). Hsp90 a was found by KEGG analysis to be involved in the regulation of the NLRP3/Caspase-1 axis in the NOD-like receptor (NLRs) signaling pathway, which is one of the classical pathways for activation of apoptosis in cells. According to the invention, researches show that the fusion peptide synthesized by the polypeptide HIBDAP and the cell-penetrating peptide can reduce the neural cell apoptosis by inhibiting the NLRP3/Caspase-1 axis under the anoxic condition, thereby playing a role in neuroprotection.

The invention aims to solve the technical problem of providing a novel brain-derived peptide, wherein the amino acid sequence of the peptide is as follows: HSQFIGYPITLFVEKER, the inventor named it HIBDAP (hypoxic immunochemical antibody associated peptide). Considering that the peptide is water-soluble, and a polypeptide with more than 6 amino acids can not generally pass through a blood brain barrier, the technical problem to be solved by the invention is to provide a fusion peptide which comprises a membrane penetrating peptide (TAT) and HIBDAP, wherein the amino acid sequence of the fusion peptide is RKKRRQRRRAHSQFIGYPITLFVEKER, and the fusion peptide is proved to be capable of penetrating through the blood brain barrier and a cell membrane into a nerve cell.

The technical problem to be solved by the invention is to provide the fusion polypeptide which can reduce the expression of NLRP3, ASC and Caspase-1 in a focal death pathway, thereby inhibiting the focal death of nerve cells and providing a new treatment method for treating neonatal HIBD.

The invention finally aims to solve the technical problem of providing the application of the fusion peptide TAT-HIBDAP in preparing a medicament for preventing or treating neonatal hypoxic-ischemic brain injury.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows: the invention provides a brain-derived peptide HIBDAP, wherein the amino acid sequence of the brain-derived peptide is HSQFIGYPITLFVEKER.

The invention further provides a fusion peptide TAT-HIBDAP, which comprises a cell-penetrating peptide and the brain-derived peptide HIBDAP.

Wherein the amino acid sequence of the fusion peptide TAT-HIBDAP is RKKRRQRRRAHSQFIGYPITLFVEKER.

The polypeptide or fusion peptide of the present invention may be synthesized by a company or may be synthesized by itself.

The invention also comprises the application of the fusion peptide TAT-HIBDAP in preparing a medicament for preventing or treating neonatal hypoxic-ischemic brain injury.

Wherein, the application detects the influence of the fusion peptide TAT-HIBDAP on the expression condition of NLRP3, ASC and/or Caspase-1.

Wherein the detection method adopts a real-time quantitative PCR and/or immunoblotting hybridization method.

Wherein, the primer pair sequences adopted in the real-time quantitative PCR are respectively as follows:

NLRP3：

F：5’-TGA AGA GTG TGA TCT GCG GAA AC-3’；

R：5’-GAA AGT CAT GTG GCT GAA GCT GT-3’；

ASC

F：5’-AGT TGA TGG TTT GCT GGA TGC T-3’；

R：5’-GGT CTG TCA CCA AGT AGG GCT G-3’；

Caspase-1

F：5’-AAC CTT GGG CTT GTC TTT-3’；

R：5’-CAG GAG GGA ATA TGT GGG-3’；

GAPDH

F：5’-AGA AGG CTG GGG CTC ATT TG-3’；

R：5’-AGG GGC CAT CCA CAG TCT TC-3’。

wherein the antibody used in the immunoblotting hybridization method is NLRP3, ASC, Caspase-1 or beta-actin respectively.

The invention also comprises a polypeptide drug, and the polypeptide drug comprises the brain-derived peptide HIBDAP or the fusion peptide TAT-HIBDAP.

The polypeptide medicine is in the form of one or more of intravenous injection, suppository, enema, gel, foam, enteric coated tablet, decoction, mixture, syrup, granule, pill, tablet, capsule and freeze-dried powder.

Has the advantages that: compared with the prior art, the invention has the following advantages:

compared with small molecule drugs, the polypeptide and the polypeptide drug thereof have the advantages of high selectivity, high efficiency and good tolerance, and compared with protein drugs, the polypeptide and the polypeptide drug thereof are simpler to produce and lower in cost. Experiments prove that the fusion peptide TAT-HIBDAP can enter cells, increase the survival rate of the cells and reduce the scorching rate under the condition of hypoxia and ischemia and has the brain protection effect.

Drawings

FIG. 1, detection of TAT-HIBDAP fusion peptide by high performance liquid chromatography;

FIG. 2, peptide entry map: fluorescence microscopy showed that TAT-HIBDAP was able to enter the cell membrane;

fig. 3, CCK8 and flow charts show: TAT-HIBDAP can increase the cell survival rate and reduce the scorching rate under the condition of hypoxia and ischemia, and OGD represents an oxygen sugar deprivation group;

FIG. 4, PCR and Western blot results show that TAT-HIBDAP can inhibit the expression of NLRP3, ASC and Caspase-1 under the condition of hypoxia and ischemia in vitro experiments; NC: a normal group; OGD: oxygen sugar deprived group; 5 μ M + OGD: oxygen sugar deprivation after treatment of polypeptide TAT-HIBDAP with the concentration of 5 mu M;

FIG. 5 shows that TAT-HIBDAP can inhibit the expression of NLRP3, ASC and Caspase-1 under the condition of hypoxia ischemia by PCR (polymerase chain reaction) results of extracting cerebral cortex on the side of hypoxia ischemia by injecting TAT-HIBDAP into abdominal cavity of newborn SD rat after hypoxia ischemia; NC: a normal group; HI: hypoxia-ischemia treated group; HI + NS: hypoxia-ischemia + saline injection group; HI +2859 nmol/kg: hypoxic ischemia and TAT-HIBDAP polypeptide treatment group with a concentration of 2859 nmol/kg.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to examples, but those skilled in the art will appreciate that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.

EXAMPLE 1 obtaining the polypeptide HIBDAP

1. Samples were selected from 4 HiBD infants in the Neonatal Intensive Care Unit (NICU) of the child Hospital affiliated to Nanjing medical university. Cerebrospinal fluid samples from the control group were taken from 4 newborns without known neurological disease who required lumbar puncture diagnosis for routine sepsis assessment. Adding protease inhibitor (Complete mini EDTA-free, Med Chem Express, USA) and storing in liquid nitrogen;

2. grinding a sample in liquid nitrogen, adding a protein lysate (8M Urea), blowing, beating and uniformly mixing, adding 1mM PMSF, 2mM EDTA and 10mM DTT with final concentration, uniformly mixing, and performing ultrasonic treatment on ice for 10 min; centrifuging at 4 ℃ and 12000r/min for 30min, taking the supernatant to a new centrifuge tube, measuring the protein concentration by a Bradford method, adding DTT to the final concentration of 10mM, and carrying out reduction reaction in a 56 ℃ water bath for 30 min. Subsequently, IAM was added to a final concentration of 55mM, and the mixture was left at room temperature in the dark for 30min for alkylation. Reducing alkane, measuring protein concentration by Bradford method, centrifuging equivalent amount of protein (128 μ g) with 10kD ultrafiltration tube at 4 deg.C and 12000r/min for 30min, ultrafiltering, and collecting penetration liquid to obtain polypeptide sample;

3. desalting the polypeptide sample by using a C18 column, and freeze-drying the desalted peptide section in vacuum;

4. dissolving all polypeptide peptide fragments by using 0.5M TEAB, marking according to the instruction of an iTRAQ-8 standard kit (AB SCIEX Inc., Framingham, MA, USA), mixing the marked samples, and then carrying out fractional separation on the mixed peptide fragments by using an Ultimate 3000HPLC system; the column used was a Durashell C18 column (5 μm,4.6X 250 mm). Elution procedure the two mobile phases were buffer a (10mM ammonium formate (PH 9.0), 2% acetonitrile in water) and buffer B (10mM ammonium formate (PH 10.0) acetonitrile), elution time and buffer A, B ratio (see table 1), 42 fractions were collected according to time and peak pattern after the samples were run through the separation column, each fraction was combined into 12 fractions according to the chromatogram, and the combined fractions were desalted on a Strata-X column and dried under vacuum.

TABLE 1 liquid phase Process

5. LC-MS/MS the vacuum dried polypeptide sample was dissolved in 2% acetonitrile/0.1% formic acid to obtain a polypeptide solution and analyzed using TripleTOF 5600plus mass spectrometer coupled to an ekstrigent nanoLC system (SCIEX, USA); the polypeptide solution was applied to a C18 capture column (5 μm, 100 μm.times.20 mm) and eluted with a 90min time gradient at a flow rate of 300nL/min on a C18 analytical column (3 μm, 75 μm.times.150 mm); the two mobile phases were buffer A (2% acetonitrile/0.1% formic acid/98% H)₂O) and buffer B (98% acetonitrile/0.1% formic acid/2% H)₂O); for IDA (information dependent acquisition), a primary mass spectrum scan was performed at an ion accumulation time of 250ms, and secondary mass spectra of 30 precursor ions were acquired at an ion accumulation time of 50 ms; MS1 spectra were collected in the range of 350-1500m/z and MS2 spectra were collected in the range of 100-1500m/z, setting the precursor ion dynamic exclusion time to 15 s;

6. protein and peptide fragments are identified by Proteinpilot software; thus obtaining the HIBDAP peptide segment, wherein the amino acid sequence of the HIBDAP peptide segment is HSQFIGYPITLFVEKER. The HIBDAP polypeptide can be synthesized by the amino acid sequence entrusted company.

7. The LC-MS/MS experiments show that the HIBDAP is obviously reduced in children suffering from HIBD, the difference multiple is-3.1, the molecular weight is 2671.5, the HIBDAP has the characteristics of strong stability, long half-life period and the like (see Table 2), precursor protein of the HIBDAP participates in the regulation of NLRP3/Caspase-1 axis in an NLR signal channel, and the polypeptide possibly plays an important role in the HIBD.

TABLE 2

Example 2 construction of the fusion peptide TAT-HIBDAP

1. Calculating the weight of each raw material based on the weight of the target polypeptide; 200mg of finished product would be expected to require 4g of crude product;

2. 3g of a resin (resin Limited, university of Nankai Tianjin) was placed in a 150ml reactor and soaked in 50ml of DCM for 2 hours;

3. washing the resin with DMF, draining, repeating the above steps for 4 times, and draining the RINK resin;

4. 1mmol of Fmoc-Arg (Pbf) -OH (the first amino acid at the C-terminal of RKKRRQRRRAHSQFIGYPITLFVEKER) +20ml of DCM +10ml of DIEA was weighed into a reactor, and then the reactor was placed in a shaker at 30 ℃ for reaction for 2 hours;

5. blocking with methanol solution (methanol: DIEA: DCM ═ 1:1:2) for half an hour, then washing 4 times with DMF, draining;

6. adding 50ml of 20 volume percent piperidine solution into a reactor, removing Fmoc protecting groups, washing with DMF for 4 times after the protection is removed, and then pumping to dry;

7. detecting a small amount of RINK resin by an indetrione method, wherein the RINK resin has color, which indicates that deprotection is successful;

8. 2mmol of Fmoc-Glu (OtBu) -OH (the second amino acid at the C-terminal of RKKRRQRRRAHSQFIGYPITLFVEKER) +40ml of HOBT +20ml of DIC are weighed into a reactor, and then the reactor is put into a shaker at 30 ℃ for reaction for 1 hour;

9. detecting a small amount of resin by an indantrione method, and if the resin has a color, indicating that condensation is incomplete, continuing to react; if the resin is colorless, the reaction is complete; after the reaction is completed, washing the resin with DMF for 4 times, and then pumping to dry;

10. 50ml of a 20% by volume piperidine solution (piperidine/DMF: 1:4) was added to the reactor and the resin was shaken for 20min on a decolorizing shaker to remove the Fmoc protecting group from the resin; washing with DMF for 4 times after the protection is removed, and then draining to detect whether the protection is removed;

11. detecting a small amount of resin by an indanthrone method, wherein the resin has color, which indicates that deprotection is successful;

12. connecting RKKRRQRRRAHSQFIGYPITLFVEKER the remaining amino acids and acetic acid in sequence according to steps 8-11;

13. cutting off all the polypeptide protecting groups by using high-concentration trifluoroacetic acid, cutting off the polypeptide protecting groups from the resin, and purifying;

14. separating the target peptide segment from impurities by a High Performance Liquid Chromatography (HPLC), freeze-drying the target peptide segment TAT-HIBDAP into powder, and sending the powder to QC quality inspection, wherein the detection HPLC and MS results both meet the requirements. The amino acid sequence of the fusion peptide TAT-HIBDAP is obtained to be RKKRRQRRRAHSQFIGYPITLFVEKER.

From Area% in table 3, it can be seen that the purity of the fusion peptide of this example is more than 95%.

TABLE 3

Peak Table

Detector A 214nm

Peak#	Ret.Time	Area	Height	Area％
					1	18.529	202070	19647	2.108
2	18.688	9133662	1063880	95.294
					3	18.888	126833	24923	1.323
4	19.329	88757	8371	0.926
					5	20.496	33371	5492	0.348
Total		9584692	1122313	100.000

Example 3 use of TAT-HIBDAP in vitro experiments

1. Dissolving synthesized TAT-HIBDAP and FITC labeled TAT-HIBDAP in sterile water at concentrations of 1 μ M, 5 μ M, 10 μ M, 20 μ M and 40 μ M respectively, adding 10 μ M FITC labeled TAT-HIBDAP into PC12 cells (ATCC, Manassas, VA, USA) for 30min, and observing the cell entry condition of the polypeptide under a fluorescence microscope, wherein the result shows that the polypeptide can enter not only cell membranes but also cell nuclei (FIG. 2);

2. PC12 cells were treated with sugar-free medium (sugar-free RPMI 1640 medium) with or without 10. mu.M TAT-HIBDAP for 1h and then placed in 2% O₂、5％CO₂And 93% N₂At 37 ℃ for 6 h;

3. inoculating PC12 cells into a 96-well plate, adding polypeptides TAT-HIBDAP with different concentrations (0, 1 mu M, 5 mu M, 10 mu M, 20 mu M and 40 mu M) when the cell density is 90%, depriving oxygen sugar for 6h, adding 10 mu L of CCK8 solution into each well under the condition of light shielding, then placing the wells into an incubator for incubation for 1h, and detecting the OD value by using an enzyme labeling instrument, wherein the result shows that the low-concentration fusion peptide TAT-HIBDAP can enhance the cell activity under the condition of hypoxia and ischemia (figure 3);

4. digesting the cells treated in the step 2 by pancreatin without EDTA, collecting the cells in a flow tube, centrifuging at 1500rpm for 5min, then discarding the supernatant, washing 2 times by 1ml of PBS, centrifuging at 1500rpm for 5min after gently blowing the cells, successively adding 300 mu L Binding Buffer, 2.5 mu L FITC and PI, suspending the mixture in a sterilized PBS Buffer solution, adjusting BD flow cytometer parameters, calculating the cell count to 10000, and calculating the average fluorescence intensity of the cells, wherein the result shows that the fusion peptide TAT-HIBDAP can reduce the cell apoptosis rate (figure 3);

5. the expression conditions of NLRP3, ASC and Caspase-1 are detected by adopting a real-time quantitative PCR and immunoblotting hybridization (Western Blot) method, and the sequences are as follows: NLRP3 (F: 5'-TGA AGA GTG TGA TCT GCG GAA AC-3'; R: 5'-GAAAGT CAT GTG GCT GAA GCT GT-3'); ASC (F: 5'-AGT TGA TGG TTT GCT GGA TGC T-3'; R: 5'-GGT CTG TCA CCA AGT AGG GCT G-3'); caspase-1 (F: 5'-AAC CTT GGG CTT GTC TTT-3'; R: 5'-CAG GAG GGA ATA TGT GGG-3'); GAPDH (F: 5'-AGA AGG CTG GGG CTC ATT TG-3'; R: 5'-AGG GGC CAT CCA CAG TCT TC-3'); antibody: NLRP3 (Cat: 19771-1-AP; Proteitech, Chicago, USA); ASC (Cat: sc-514414; Santa Cruz, Calif., USA); caspase-1 (Cat: sc-514; Santa Cruz, Calif., USA), beta-actin (Cat: 8457S; Cell Signaling Technology, MA, USA);

real-time quantitative PCR reaction system and reaction conditions:

reverse transcription:

reverse transcription was performed by Hiscript II Q RT Supermix for qPCR (R222-01).

The reverse transcription reagent is prepared according to the following system: (10. mu.L/well)

Reverse transcription of mRNA

And (4) centrifuging after uniformly mixing by vortex, adding into the eight-connected calandria respectively, and starting to react.

After the reaction is finished, the obtained cDNA sample can be placed on ice or stored at-20 ℃.

2.6.2 real-time quantitative PCR reaction System

PCR was performed by AceQ qPCR SYBER Green Master Mix (R131-03, Vazyme, Nanjing, China). Taking GAPDH as an internal reference.

The PCR reagent is prepared according to the following system: (10. mu.L/well)

And (4) carrying out vortex mixing, centrifuging, adding the samples into a 96-well plate respectively, and starting reaction after centrifuging.

Western blot step: protein was extracted by adding protein lysate, protein concentration was measured using BCA kit (Pierce, Rockford, IL, USA), loading buffer (Abcam, Cambridge, MA, UK) was added, and cooking was performed at 100 ℃ for 5 min. Samples were separated on 10% SDS-PAGE, transferred onto PVDF membrane (Millipore, MA, USA), blocked with skimmed milk powder at room temperature, followed by incubation of primary antibodies NLRP3 (Cat: 19771-1-AP; Proteitech, Chicago, USA), ASC (Cat: sc-514414; Santa Cruz, CA, USA), Caspase-1 (Cat: sc-514; Santa Cruz, CA, USA), beta-actin (Cat: 8457S; Cell Signaling Technology, MA, USA) overnight at 4 ℃. Wash 5 times with TBST containing 0.1% Tween 20 for 10 minutes each, then treat 1h with a secondary antibody conjugated with horseradish peroxidase at room temperature for 5 times for 10 minutes each. Finally, the measurement was carried out by a developing instrument and quantified by an image analysis system (image J).

PCR and Western blot results show that the expression of NLRP3, ASC and Caspase-1 is increased when oxygen sugar is deprived, the expression is reduced when TAT-HIBDAP is added for pretreatment, and the TAT-HIBDAP can inhibit the expression of NLRP3, ASC and Caspase-1 in a focal death pathway under the condition of hypoxia and ischemia (figure 4) and has potential brain protection effect.

Example 4 animal experiments

1. Construction of neonatal rat ischemia-Hypoxia (HI) model

Taking 7-day-old SD clean-grade rats, making male and female halves, weighing 12-18g, anesthetizing sham-operated rats with 5% chloral hydrate (1mL/100g), cutting only neck skin and muscle, exposing left common carotid artery, but no artery ligation and no hypoxia treatment are carried out, after the HI model group rat is anesthetized by chloral hydrate, the left common carotid artery is separated and permanently ligated, then the rat is placed into a hypoxia chamber containing 8 percent of oxygen and 92 percent of nitrogen for 2h, the temperature and the humidity are respectively kept at 37 ℃ and 50-80 percent, then the rat is caged with a mother rat, the HI and the rat of the experimental group injected with saline in the abdominal cavity are treated in the same way, the treatment of rats in the experimental group which is injected with 0.005ml/g of normal saline, HI and polypeptide in the abdominal cavity before the combination of the cages and the maternal mice is the same as the treatment of rats in the experimental group, the anterior abdominal cavity of the mother rat is injected with 0.005ml/g TAT-HIBDAP polypeptide solution with the concentration of 2359 nmol/kg. Newborn rats were sacrificed at 48h postoperatively by decapitation. 6-8 ligature lateral brain tissues are reserved in each group, and the separated cortex is stored in liquid nitrogen for later use.

2. And (3) detecting the expression conditions of NLRP3, ASC and Caspase-1 in the cortex by adopting a real-time quantitative PCR method.

PCR results show that NLRP3, ASC and Caspase-1 are remarkably increased after HI treatment, and NLRP3, ASC and Caspase-1 expression is reduced when TAT-HIBDAP is added after HI treatment, so that TAT-HIBDAP can inhibit the expression of NLRP3, ASC and Caspase-1 in a apoptosis pathway after HI (figure 5) and has a brain protection effect.

Sequence listing

<110> Nanjing City children hospital

Application of brain-derived peptide HIBDAP or fusion peptide TAT-HIBDAP in preparation of medicine for preventing or treating neonatal hypoxic-ischemic brain injury

<160> 10

<170> SIPOSequenceListing 1.0

<210> 1

<211> 17

<212> PRT

<213> brain peptide HIBDAP (artificial sequence)

<400> 1

His Ser Gln Phe Ile Gly Tyr Pro Ile Thr Leu Phe Val Glu Lys Glu

1 5 10 15

Arg

<210> 2

<211> 27

<212> PRT

<213> TAT-HIBDAP(Artificial Sequence)

<400> 2

Arg Lys Lys Arg Arg Gln Arg Arg Arg Ala His Ser Gln Phe Ile Gly

1 5 10 15

Tyr Pro Ile Thr Leu Phe Val Glu Lys Glu Arg

20 25

<210> 3

<211> 23

<212> DNA

<213> NLRP3 F(Artificial Sequence)

<400> 3

tgaagagtgt gatctgcgga aac 23

<210> 4

<211> 23

<212> DNA

<213> NLRP3 R(Artificial Sequence)

<400> 4

gaaagtcatg tggctgaagc tgt 23

<210> 5

<211> 22

<212> DNA

<213> ASC F(Artificial Sequence)

<400> 5

ggtctgtcac caagtagggc tg 22

<210> 6

<211> 22

<212> DNA

<213> ASC R(Artificial Sequence)

<400> 6

ggtctgtcac caagtagggc tg 22

<210> 7

<211> 18

<212> DNA

<213> Caspase-1 F(Artificial Sequence)

<400> 7

aaccttgggc ttgtcttt 18

<210> 8

<211> 18

<212> DNA

<213> Caspase-1 R(Artificial Sequence)

<400> 8

caggagggaa tatgtggg 18

<210> 9

<211> 20

<212> DNA

<213> GAPDH F(Artificial Sequence)

<400> 9

agaaggctgg ggctcatttg 20

<210> 10

<211> 20

<212> DNA

<213> GAPDH R(Artificial Sequence)

<400> 10

aggggccatc cacagtcttc 20