Pesticide composition and application thereof

文档序号：752487 发布日期：2021-04-06 浏览：40次中文

阅读说明：本技术 一种农药组合物及其应用 (Pesticide composition and application thereof ) 是由郭一韬张正虎朱瑞庭孙伟于 2020-12-25 设计创作，主要内容包括：本公开涉及一种农药组合物及其应用,该农药组合物为双层片剂,上层含有活性成分,所述活性成分包括百菌清、28-高芸苔素内酯和阿氏芽孢杆菌；所述阿氏芽孢杆菌的保藏编号为CGMCC No:17204；下层含有活性成分,所属活性成分包括百菌清。所述应用指的是的所述农药组合物在防治水稻病害和水稻增产中的应用。本发明的农药组合物中百菌清、28-高芸苔素内酯和阿氏芽孢杆菌具有协同增效作用,用量低、防效好并且能同时防治多种病虫害；该农药组合物在生产和使用过程中均以水为基质,低毒环保。(The present disclosure relates to a pesticide composition and an application thereof, the pesticide composition is a double-layer tablet, the upper layer contains active ingredients, and the active ingredients comprise chlorothalonil, 28-high brassinolide and bacillus aryabhattai; the preservation number of the Bacillus aryabhattai is CGMCC No. 17204; the lower layer contains active ingredients, including chlorothalonil. The application refers to the application of the pesticide composition in preventing and treating rice diseases and increasing the yield of rice. In the pesticide composition, chlorothalonil, 28-high brassinolide and bacillus aryabhattai have a synergistic effect, the dosage is low, the control effect is good, and various plant diseases and insect pests can be controlled simultaneously; the pesticide composition takes water as a matrix in the production and use processes, and is low in toxicity and environment-friendly.)

1. A pesticide composition is characterized by comprising active ingredients, wherein the active ingredients comprise chlorothalonil, 28-high brassinolide and Bacillus aryabhattai; the preservation number of the Bacillus aryabhattai is CGMCC No. 17204.

2. The pesticide composition as set forth in claim 1, which is a bilayer tablet, the upper active ingredient comprises chlorothalonil, 28-homobrassinolide and Bacillus aryabhattai, the lower active ingredient comprises chlorothalonil, and the weight ratio of the upper chlorothalonil to the lower chlorothalonil is 1: (1-5).

3. The pesticide composition as claimed in claim 2, wherein the weight ratio of chlorothalonil, 28-homobrassinolide and bacillus aryabhattai which are active ingredients in the upper layer of the pesticide composition is 1: (0.0001-0.001): (0.1-20); the number of viable bacteria of the Bacillus aryabhattai is 1 multiplied by 10⁷cfu/g～1×10¹²cfu/g。

4. The pesticide composition as claimed in claim 2 or 3, wherein the weight ratio of the active ingredients of chlorothalonil, 28-homobrassinolide and Bacillus aryabhattai on the upper layer of the pesticide composition is 1: (0.0001-0.001): (1-10); the number of viable bacteria of the Bacillus aryabhattai is 1 multiplied by 10⁸cfu/g～1×10⁹cfu/g。

5. The pesticide composition as claimed in any one of claims 1 to 4, wherein the pesticide composition further comprises an auxiliary component, and the weight ratio of the active component to the auxiliary component is 1: (0.1-20).

6. The agricultural chemical composition according to claim 5, wherein the auxiliary ingredient comprises at least one selected from the group consisting of a dispersing agent, a wetting agent, a binder, a disintegrant, a flow regulator and a filler.

7. The pesticide composition as set forth in claim 6, wherein the weight ratio of the active ingredient to the dispersing agent, the wetting agent, the binder, the disintegrant, the flow regulator is 1: (0.005-5): (0.005-2): (0.01-5): (1-20): (0.1-10).

8. The pesticide composition as set forth in any one of claims 1 to 7, wherein the formulation of the pesticide composition is a bilayer tablet, wherein the upper layer is an effervescent tablet and the lower layer is a direct use tablet; the mode of application of the pesticide composition is broadcast application.

9. The use of the pesticidal composition of any one of claims 1 to 8 for controlling rice diseases and increasing rice yield.

10. The use according to claim 9, wherein the rice disease comprises at least one selected from the group consisting of rice leaf blight, rice blast, and false smut.

Technical Field

The disclosure relates to the technical field of pesticides, and particularly relates to a pesticide composition and application thereof.

Background

The disease control of rice as an important grain crop in China is always an important task in the agricultural development in China, wherein the fungal diseases of rice are various and seriously harmful, and have great influence on the yield and the quality of the rice.

Common fungal diseases of rice include sheath blight disease, rice blast and false smut.

Sheath blight belongs to soil-borne diseases and is caused by infection of rhizoctonia solani, which is easy to occur and prevail under high-temperature and high-humidity conditions. The sheath blight disease usually causes water-stain-like elliptic and cloud-like disease spots directly near the water surface, and the disease spots gradually become green or light brown, and hyphae are generated and infect nearby rice plants. The disease is widely distributed in various rice production areas in the world, and the harm is most prominent in the high rice production areas. Mainly causes sheath blight and leaf blight, reduces the maturing rate of rice, reduces the grain weight, generally reduces the yield by 10-20 percent, and can reach more than 30 percent in serious cases.

The blast disease is usually caused by infection with fungi of the subdivision Saccharomycotina or Deuteromycotina, and is mainly conidia. After the conidium infects rice plants, the conidium gradually grows among rice cells, and new spores are gradually formed and released at the positions of disease spots of rice leaves, so that a cycle of disease period is completed. The rice blast can occur in various growth stages of rice, and can be divided into seedling plague, leaf plague, joint plague, neck plague and grain plague according to different attack stages and attack parts. Leaf plague and neck plague are the most common and have large harm, and when the leaf plague is serious, the whole field is burnt, plants are dwarf, new leaves are difficult to grow, and heading is difficult. When the neck blast in heading stage is serious, a large amount of white ears or shrivelled grains are caused.

False smut occurs only in ears, and generally occurs from flowering to maturity of rice. The early diseased grain glumes are opened to expose light yellow small blocky bulges with cyan, then the diseased grain glumes gradually expand to wrap the whole grains, the color of the diseased grain glumes is dark green, and finally the diseased grain glumes crack to emit black powder. False smut not only seriously affects the yield of rice, but also pollutes rice. The toxin can be generated to directly harm the human health, so that the prevention and the treatment of the rice false smut are very necessary.

Disclosure of Invention

The present invention provides a pesticide composition and an application thereof, which aims to solve the problems that the current bactericide needs to be applied for many times, is easy to generate drug resistance, and the pesticide effect is reduced, so that the yield and the quality of rice are affected.

In order to achieve the above object, the present disclosure provides in a first aspect a pesticidal composition comprising active ingredients including chlorothalonil, 28-homobrassinolide and bacillus aryabhattai; the preservation number of the Bacillus aryabhattai is CGMCC No. 17204.

The second aspect of the disclosure provides an application of the pesticide composition in preventing and treating rice diseases and increasing rice yield.

Through the technical scheme, the active ingredients of chlorothalonil, 28-high brassinolide and bacillus aryabhattai in the upper layer of the tablet in the pesticide composition have a synergistic effect, the dosage is low, the control effect is good, various plant diseases and insect pests can be controlled simultaneously, and the active ingredient of chlorothalonil in the lower layer of the tablet has a slow release effect. The pesticide composition can be used for continuously controlling rice sheath blight disease, rice blast and false smut once, and is not required to be used for multiple times in the rice growth period. In addition, the pesticide composition takes water as a matrix in the production and use processes, and is low in toxicity and environment-friendly.

Additional features and advantages of the invention will be set forth in the detailed description which follows.

Biological material preservation information

Bacillus aryabhattai, classified and named Bacillus aryabhattai, is preserved in the China general microbiological culture Collection center of the Committee for culture Collection of microorganisms, and the preservation addresses are as follows: the No. 3 Xilu Beijing, Chaoyang, has a preservation date of 2019, 16 months, and a preservation number of CGMCC No. 17204.

Detailed Description

The following describes in detail specific embodiments of the present disclosure. It should be understood that the detailed description and specific examples, while indicating the present disclosure, are given by way of illustration and explanation only, not limitation.

In a first aspect, the present disclosure provides a pesticide composition comprising active ingredients including chlorothalonil, 28-homobrassinolide and bacillus aryabhattai; the preservation number of the Bacillus aryabhattai is CGMCC No. 17204.

Wherein chlorothalonil is trade name of tetrachloroisophthalonitrile (2, 4, 5, 6-tetrachloro-1, 3-phthalonitrile), and has a molecular formula: c₈N₂Cl₄Relative molecular weight: 265.91 having the English name chlorothalonil and CAS number 1897-45-6, and the structural formula is shown in formula (1):

28-homobrassinolide is a trade name for (22R, 23R, 24S) -2 α, 3 α, 22, 23-tetrahydroxy-24-ethyl- β -homo-7-oxa-5 α -stan-6-one, formula C₂₉H₅₀O₆Relative molecular mass 494.8, 28-homobrassinolide in English name, CAS number 82373-95-3, and structural formula shown in formula (2):

the Bacillus aryabhattai (Bacillus aryabhattai) in the disclosure is selected from (1) Bacillus aryabhattai (Bacillus aryabhattai) deposited in the common microorganism center of the China Committee for culture Collection of microorganisms, wherein the preservation numbers are: CGMCC No. 17204; the preservation time is as follows: year 2019, month 01, day 16; (2) progeny, mutants or derivatives of the above strains.

According to the present disclosure, the pesticide composition is a bilayer tablet, the upper active ingredient comprises chlorothalonil, 28-homobrassinolide and bacillus aryabhattai, the lower active ingredient comprises chlorothalonil, and the weight ratio of the upper chlorothalonil to the lower chlorothalonil is 1: (1-5).

According to the disclosure, the pesticide composition comprises chlorothalonil and 28-high brassin as upper active ingredientsThe weight ratio of the lactone to the bacillus aryabhattai is 1: (0.0001-0.001): (0.1-20); the number of viable bacteria of the Bacillus aryabhattai is 1 multiplied by 10⁷cfu/g～1×10¹²cfu/g。

According to the present disclosure, the weight ratio of chlorothalonil, 28-homobrassinolide and bacillus aryabhattai, which are active ingredients in the upper layer of the pesticide composition, is 1: (0.0001-0.001): (1-10); the number of viable bacteria of the Bacillus aryabhattai is 1 multiplied by 10⁸cfu/g～1×10⁹cfu/g。

According to the disclosure, the pesticide composition further comprises auxiliary components, and the weight ratio of the active components to the auxiliary components is 1: (0.1-20).

According to the present disclosure, wherein the auxiliary ingredient includes at least one selected from a dispersing agent, a wetting agent, a film-forming agent, a binder, a disintegrant, a flow regulator, and a filler.

According to the present disclosure, wherein the disintegrant further comprises an acidic effervescent disintegrant, a basic effervescent disintegrant.

According to the present disclosure, wherein the auxiliary ingredients include a dispersing agent, a wetting agent, a binder, a disintegrant, a flow regulator; the weight ratio of the active ingredients to the dispersing agent, the wetting agent, the adhesive, the disintegrating agent and the flow regulator is 1: (0.005-5): (0.005-2): (0.01-5): (1-20): (0.1-10).

According to the disclosure, the dosage form of the pesticide composition is a double-layer tablet, wherein the upper layer is an effervescent tablet and the lower layer is a direct-use tablet.

According to the present disclosure, wherein the mode of application of the pesticidal composition is broadcasting.

The second aspect of the invention provides application of the pesticide composition in preventing and treating rice diseases and increasing rice yield.

According to the present disclosure, the rice disease includes at least one selected from sheath blight disease, rice blast, and false smut.

Effervescent tablets generally consist of active ingredients, wetting agents, binders, disintegrants, flow regulators, fillers; the disintegrant also comprises an acidic effervescent disintegrant and a basic effervescent disintegrant; the wetting agent is selected from at least one of water, ethanol and sodium dodecyl sulfate; the adhesive is selected from at least one of starch slurry, carboxymethyl cellulose, hydroxypropyl methyl cellulose, carboxymethyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, dextrin, sugar powder, sodium alginate, PEG4000 and PEG 6000; the disintegrant is at least one selected from dry starch, sodium carboxymethyl starch, hydroxypropyl starch, low-substituted hydroxypropyl cellulose, croscarmellose sodium and crospolyvinylpyrrolidone; the acidic effervescent disintegrant is at least one selected from citric acid, tartaric acid and citric acid; the alkaline effervescent disintegrant is selected from at least one of sodium bicarbonate and sodium carbonate; the flow regulator is at least one selected from stearic acid, calcium stearate, magnesium stearate, talcum powder, hydrogenated vegetable oil, PEG4000, PEG6000 and sodium lauryl sulfate; the filler is selected from at least one of starch, sugar powder, dextrin, compressible starch, lactose, microcrystalline cellulose, calcium sulfate, kaolin, calcium carbonate and light magnesium oxide.

Direct use tablets typically consist of the active ingredient, a wetting agent, a dispersing agent, a binder, a flow regulator, and a filler. The wetting agent is selected from at least one of sodium dodecyl sulfate and sodium dodecyl benzene sulfonate; the dispersant is selected from at least one of polyisobutylene succinic anhydride derivatives, acrylic acid grafted copolymers and sodium lignosulfonate; the adhesive is selected from at least one of starch, carboxymethyl cellulose, hydroxypropyl methyl cellulose, carboxymethyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, dextrin, sugar powder, sodium alginate, PEG4000 and PEG 6000; the flow regulator is at least one selected from stearic acid, calcium stearate, magnesium stearate, talcum powder, hydrogenated vegetable oil, PEG4000, PEG6000 and sodium lauryl sulfate; the filler is selected from at least one of starch, sugar powder, dextrin, compressible starch, lactose, microcrystalline cellulose, calcium sulfate, kaolin, calcium carbonate and light magnesium oxide.

The present disclosure is further illustrated by the following examples, but is not to be construed as being limited thereby.

In the following examples and comparative examples, chlorothalonil was produced by this company; 28-high brassinolide was purchased from Kangshengyuan (Zhaoqing) Biotech limited; bacillus aryabhattai was purchased from Kangshengyuan (Zhaoqing) Biotech limited; carbendazim was purchased from Jiangsu Huifeng biological agriculture GmbH; polyisobutylene succinic anhydride derivatives, acrylic acid grafted copolymers were purchased from the prochloraz chemical (shanghai) limited; sodium lauryl sulfate, polyvinylpyrrolidone, PEG6000, croscarmellose sodium, citric acid, sodium carbonate, magnesium stearate, calcium sulfate, microcrystalline cellulose were purchased from Beijing chemical plants; the rest is a commercial product.

Example 1