阅读说明：本技术 一种蜡蚧轮枝菌与甲氨基阿维菌素苯甲酸盐的复合制剂 (Compound preparation of verticillium lecanii and emamectin benzoate ) 是由 张宏 张鹏飞 张瑞 于 2020-11-26 设计创作，主要内容包括：本发明公开了一种蜡蚧轮枝菌与甲氨基阿维菌素苯甲酸盐的复合制剂。所述蜡蚧轮枝菌复合制剂的活性成分为蜡蚧轮枝菌和甲氨基阿维菌素苯甲酸盐,蜡蚧轮枝菌为蜡蚧轮枝菌Beijing-P菌株；所述复合剂由所述蜡蚧轮枝菌的分生孢子悬浮液和甲氨基阿维菌素苯甲酸盐微乳剂复配得到。本发明复配制剂对于植物病虫害和螨害的防治,相对于两种单剂,具有协同增效的效果,且显著提高了蜡蚧轮枝菌的防治效果,因此能够提高蜡蚧轮枝菌的速效性和持久性,减少化学农药的施用量,解决环境污染的问题。(The invention discloses a compound preparation of verticillium lecanii and emamectin benzoate. The active ingredients of the verticillium lecanii compound preparation are verticillium lecanii and emamectin benzoate, and the verticillium lecanii is a Beijing-P strain of verticillium lecanii; the complexing agent is obtained by compounding conidium suspension of Verticillium lecanii and emamectin benzoate microemulsion. Compared with two single agents, the compound preparation has a synergistic effect on the prevention and treatment of plant diseases and insect pests and mite pests, and obviously improves the prevention and treatment effect of verticillium lecanii, so that the quick-acting property and the durability of the verticillium lecanii can be improved, the application amount of chemical pesticide is reduced, and the problem of environmental pollution is solved.)

1. A compound preparation of Verticillium lecanii comprises active ingredients of Verticillium lecanii and emamectin benzoate.

2. The complex formulation of claim 1, wherein: the Verticillium lecanii is Verticillium lecanii Beijing-P strain.

3. Composite formulation according to claim 1 or 2, characterized in that: the complexing agent is obtained by compounding conidium suspension of Verticillium lecanii and emamectin benzoate microemulsion.

4. The complex formulation according to claim 2 or 3, characterized in that: in the Verticillium lecanii compound preparation, the concentration of conidia of the Verticillium lecanii is 1 × 10⁷～1×10⁸Spores/ml.

5. The complex formulation according to claim 3 or 4, characterized in that: the emamectin benzoate microemulsion is a 2% emamectin benzoate microemulsion, and the dilution multiple of the emamectin benzoate microemulsion is 2000-8000.

6. Use of the verticillium lecanii composite formulation of any one of claims 1 to 5 for controlling plant diseases and pests and mite pests.

7. An agent for controlling plant diseases and insect pests and mite pests, wherein the active ingredient of the agent is the Verticillium lecanii composite preparation as defined in any one of claims 1 to 5.

Technical Field

The invention relates to a compound preparation of verticillium lecanii and emamectin benzoate, belonging to the field of plant disease and insect control in the agricultural field.

Background

Verticillium lecanii is an important pathogenic fungus. The strain has wide host range, has pathogenicity on scale insects, aphids, mites, whiteflies, thrips, nematodes, lepidoptera pests and the like, and also has an inhibiting effect on plant pathogenic fungi such as rust disease bacteria and powdery mildew disease bacteria. The verticillium lecanii is safe to human beings and natural enemies of pests, is an environment-friendly pathogenic microorganism, and has important application value in green prevention and control of agricultural and forestry plant diseases and insect pests.

Microbial pesticides also have the disadvantages of low pesticide effect, short shelf life and the like. Although the application of chemical pesticides brings problems of environmental pollution, pesticide residue and the like, the pesticide effect is high, the shelf life is long, and the pesticide can be mutually compensated with microbial pesticides. Therefore, if the chemical pesticide and the microbial pesticide are applied in a compounding way, the pesticide effect can be ensured, and the purpose of reducing the application amount of the chemical pesticide can be achieved, so that the method is an important development direction at present and in the future. However, many chemical pesticides are not formulated with microbial pesticides because of their inhibitory effect on microorganisms.

Disclosure of Invention

Experiments show that the emamectin benzoate has a weak inhibition effect on verticillium lecanii, so that the verticillium lecanii and the emamectin benzoate can be compounded, have a synergistic effect, and can be used for preventing and treating plant diseases and insect pests and mite pests.

The active ingredients of the compound preparation provided by the invention are verticillium lecanii and emamectin benzoate.

The present invention specifically uses a strain of Verticillium lecanii Beijing-P isolated from Trialeurodes vaporariorum by a method described in The literature (The effects of The same storage conditions on The living environment of Lecanicillium lecanii con to white (Homoptera: Trialeurodes vaporarium), Aidong Chen, Zhangyan Shi and Long Zhang, Biocontrol Science and Technology, Vol.18, No.3,2008, 267-278).

Specifically, the compound preparation is obtained by compounding a conidium suspension of Verticillium lecanii and an emamectin benzoate microemulsion, wherein the emamectin benzoate microemulsion is a microemulsion prepared by diluting 2% of emamectin benzoate microemulsion by 2000-8000, and the concentration of conidia of Verticillium lecanii in the compound preparation can be 1 × 10⁷～1×10⁸Spores/ml;

the dilution multiple of the 2% emamectin benzoate microemulsion is preferably 2000-4000, 4000-8000, 2000, 4000 or 8000;

the 2% emamectin benzoate microemulsion refers to a microemulsion of an emamectin benzoate raw drug, and the mass percentage content of the emamectin benzoate raw drug is 2%.

The verticillium lecanii compound agent provided by the invention can prevent and control plant diseases and insect pests and mite pests;

wherein the diseases mainly comprise rust diseases and powdery mildew diseases; the pests comprise scales, aphids, whiteflies, thrips, nematodes and the like; the mite damage is phytophagous mites.

After the verticillium lecanii is compounded with the emamectin benzoate, the inhibition rate of the emamectin benzoate on the hypha growth of the verticillium lecanii is lower than 40 percent, and the inhibition rate of the emamectin benzoate on the spore germination of the verticillium lecanii is lower than 30 percent, which shows that the verticillium lecanii has stronger drug resistance on the emamectin benzoate, and the verticillium lecanii and the emamectin benzoate have good compatibility. Compared with two single agents, the compound preparation has a synergistic effect on the prevention and treatment of plant diseases and insect pests and mite pests, and obviously improves the prevention and treatment effect of verticillium lecanii, so that the quick-acting property and the durability of the verticillium lecanii can be improved, the application amount of chemical pesticide is reduced, and the problem of environmental pollution is solved.

Drawings

FIG. 1 shows the colony morphology of Verticillium lecanii Beijing-P strain grown for 27 days on PDA medium containing emamectin benzoate at different dilution.

FIG. 2 shows the effect of emamectin benzoate on the growth of Verticillium lecanii hyphae at different dilution times.

FIG. 3 shows the inhibition effect of emamectin benzoate at different dilution times on Verticillium lecanii spore germination.

Detailed Description

The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

Example 1 resistance of Verticillium lecanii to 2% Emamectin benzoate microemulsion

Mixing conidium suspension of Verticillium lecanii Beijing-P strain with 2% emamectin benzoate microemulsion, wherein the dilution times of the 2% emamectin benzoate microemulsion are 500, 1000, 2000, 4000 and 8000 times, and the concentration of conidium in the obtained compound agent is 2 × 10⁷Spores/ml.

The compound preparation is cultured on a PDA culture medium, after the compound preparation is cultured for 27 days, the result of the hypha community form of the verticillium lecanii is shown in figure 1, as can be seen from figure 1, the diameter of a bacterial colony of the verticillium lecanii after the conidia grow for 27 days on the PDA culture medium respectively containing 2 percent methylamino abamectin benzoate microemulsion with the final dilution times of 500, 1000, 2000, 4000 and 8000 is slightly smaller than that of a control without pesticide, but no obvious difference exists, which indicates that the verticillium lecanii Beijing-P bacterial strain has stronger drug resistance to the methylamino abamectin benzoate, and indicates that the verticillium lecanii and the Beijing-P bacterial strain have good compatibility.

The statistical results of the inhibition effect of emamectin benzoate with different dilution times on the growth of the verticillium lecanii Beijing-P strain hyphae are shown in fig. 2, the culture time is 5 days, and it can be seen that in the emamectin benzoate treatment with 5 dilution times, the emamectin benzoate with the dilution times of 2000 times, 4000 times and 8000 times has very low inhibition rate on the growth of the verticillium lecanii hyphae, the highest value does not exceed 40%, and two high-concentration treatments (500 times and 1000 times) show relatively high inhibition rate, and the highest value does not exceed 50%.

Wherein, the calculation formula of the hypha growth inhibition rate is shown as the formula (1):

in the formula I₁Showing the rate of inhibition of hyphal growth, c₁Denotes the diameter of the control colony, p₁The diameter of the treated colonies is indicated. This box plot shows the minimum, lower quartile, median, upper quartile and maximum for all replicates in each treatment. Before statistical analysis, square root arcsine conversion is firstly carried out on data, then single-factor variance analysis is carried out on the data, and finally multiple comparison is carried out.

The statistical result of the inhibition effect of emamectin benzoate with different dilution times on spore germination of verticillium lecanii Beijing-P strain is shown in figure 3, the culture time is 5 days, and it can be seen that the inhibition rates of emamectin benzoate with different dilution times on spore germination of verticillium lecanii are all very low, and the inhibition rate of the highest concentration treatment (500 times) is not over 40%, indicating that the two have good compatibility.

Wherein, the calculation formula of the spore germination inhibition rate is shown as the formula (2):

in the formula I₂Indicating the inhibition rate of spore germination, c₂Denotes the germination rate, p, of control spores₂The diameter of the treated spores is indicated. This box plot shows the minimum, lower quartile, median, upper quartile and maximum for all replicates in each treatment. Before statistical analysis, square root arcsine conversion is firstly carried out on the dataAnd then carrying out one-factor analysis of variance on the data, and finally carrying out multiple comparison.