阅读说明：本技术 一种祛痘添加剂及其应用 (Acne-removing additive and application thereof ) 是由 谭贺洵 申国辉 胡海宏 于 2020-12-16 设计创作，主要内容包括：本发明提供了祛痘添加剂及其应用,所述祛痘添加剂包括植物抗菌肽15份-30份、动物抗菌肽8份-12份、阴离子脂质体的磷脂10份-13份、稳定剂20份-35份、pH调节剂0.1份-3份、赋形剂1份-5份、复合抗氧剂1份-6份、缓冲剂25份-45份。通过将稳定的植物天然多肽结构与动物天然抗菌肽协同作用,能表现出更优异的抗菌性能,增加添加剂对细菌外排机制的干扰,起到显著的消炎、镇静肌肤的作用；植物抗菌肽、动物抗菌肽和阴离子脂质体的磷脂联合作用于痤疮丙酸杆菌,降低痤疮丙酸杆菌产生耐受性,降低祛痘添加剂的最低抑菌浓度,保证祛痘效果。另外,将植物抗菌肽、动物抗菌肽、稳定剂、赋形剂、复合抗氧剂以及缓冲剂复配,增加祛痘添加剂的环境抗性。(The invention provides an acne-removing additive and application thereof, wherein the acne-removing additive comprises 15-30 parts of plant antibacterial peptide, 8-12 parts of animal antibacterial peptide, 10-13 parts of phospholipid of anionic liposome, 20-35 parts of stabilizer, 0.1-3 parts of pH regulator, 1-5 parts of excipient, 1-6 parts of composite antioxidant and 25-45 parts of buffer. Through the synergistic effect of the stable plant natural polypeptide structure and the animal natural antibacterial peptide, the antibacterial agent can show more excellent antibacterial performance, increase the interference of the additive on the bacterial efflux mechanism, and play a remarkable role in diminishing inflammation and calming the skin; the plant antibacterial peptide, the animal antibacterial peptide and the phospholipid of the anionic liposome act on the propionibacterium acnes in a combined manner, so that the tolerance of the propionibacterium acnes is reduced, the minimum inhibitory concentration of the acne removing additive is reduced, and the acne removing effect is ensured. In addition, the plant antibacterial peptide, the animal antibacterial peptide, the stabilizer, the excipient, the composite antioxidant and the buffering agent are compounded, so that the environmental resistance of the acne-removing additive is increased.)

1. An acne-removing additive, characterized in that it comprises:

the plant antibacterial peptide is obtained by extracting a mixture of barley, alfalfa, peas and sesame and then carrying out enzymatic hydrolysis by using a complex enzyme, wherein the weight part ratio of the added plant antibacterial peptide is 15-30 parts;

an animal antibacterial peptide, which is added by weight portion of 8-12 portions;

the phospholipid of anionic liposome is added in a weight ratio of 10 to 13;

a stabilizer, which is added in a weight ratio of 20 to 35;

a pH regulator, which is added in a weight ratio of 0.1 to 3;

an excipient, which is added in a weight ratio of 1 to 5;

1-6 parts of a composite antioxidant, wherein the weight part ratio of the composite antioxidant is 1-6 parts;

a buffering agent, which is added by weight part ratio of 25-45 parts.

2. The additive for removing acne according to claim 1, wherein the barley, the alfalfa, the pea and the sesame are mixed according to a weight ratio of 1:1-5:2-7: 1-4.

3. The additive for removing acne according to claim 1, wherein the animal antibacterial peptide is obtained by extracting artificial bred silkworms with 1, 2-propanediol.

4. The acne treatment additive according to claim 1, wherein the phospholipid of the anionic liposome is one or more of phosphatidic acid, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol.

5. The acne treatment additive according to claim 1, wherein the stabilizer is one or more of tween-80, Pluronic F68, Pluronic F88.

6. The acne treatment additive according to claim 1, wherein the pH adjusting agent is citric acid.

7. The additive for removing acne according to claim 1, wherein the excipient is one or more of mannitol, sorbitol, trehalose, chitosan.

8. The acne treatment additive according to claim 7, wherein the preparation method of the acne treatment additive is as follows: mixing the phospholipid of the anionic liposome, the excipient and the buffering agent in advance according to the weight part ratio, adding the plant antibacterial peptide, the animal antibacterial peptide, the stabilizing agent and the composite antioxidant under the stirring condition, uniformly dispersing, adjusting the pH to 6.5-7.0 by using a pH regulator, standing for 5-10 h, and carrying out vacuum freeze drying.

9. Use of an anti-acne additive according to any of claims 1 to 8 in the preparation of a cosmetic.

10. The use of claim 9, wherein the cosmetic is one of a cream, an emulsion, and a lotion.

Technical Field

The invention relates to the field of preparation of cosmetic raw materials, and particularly relates to an acne-removing additive and application thereof.

Background

With the improvement of living standard of people, people pay more attention to skin care and pursue higher quality of cosmetics. The cosmetic provides dual anti-aging effects, can stimulate cell neogenesis energy, simultaneously promotes collagen synthesis, improves the texture and the skin color uniformity of the skin surface layer, and makes the skin more delicate, so that it is very important to have a bottle of good cosmetic. Most of the existing cosmetics are prepared by pure chemical agents, the components are complex, the efficacy is difficult to predict, the absorption rate is low, and the requirements on the cosmetics are higher especially for acne skin. The acne skin is a common chronic inflammatory dermatosis, skin tissues of the acne skin contain more propionibacterium acnes, and the propionibacterium acnes can propagate vigorously on the skin, so that secretion and blockage of grease are aggravated, a large amount of acnes are expressed, and the appearance is seriously influenced. However, the existing acne removing products on the market are poor in quality and relatively large in general irritation, and the skin with multiple acnes cannot play an effective anti-inflammatory and sedative effect, and even the appearance of the skin with the acnes is aggravated; in addition, some acne removing products in the prior art have single efficacy, do not have excellent moisturizing, oxidation resistance and other effects, have poor resistance to the surrounding environment and are not beneficial to storage of the acne removing products. Therefore, further creative research on acne-removing products is needed.

In conclusion, the above problems to be solved are still needed in the field of acne-removing products.

Disclosure of Invention

Based on the above, in order to solve the problems of large irritation, poor effect, single effect and unfavorable preservation of an acne removing product, the invention provides an acne removing additive and application thereof, and the specific technical scheme is as follows:

an acne-removing additive comprising:

the plant antibacterial peptide is obtained by extracting a mixture of barley, alfalfa, peas and sesame and then carrying out enzymatic hydrolysis by using a complex enzyme, wherein the weight part ratio of the added plant antibacterial peptide is 15-30 parts;

an animal antibacterial peptide, which is added by weight portion of 8-12 portions;

the phospholipid of anionic liposome is added in a weight ratio of 10 to 13;

a stabilizer, which is added in a weight ratio of 20 to 35;

a pH regulator, which is added in a weight ratio of 0.1 to 3;

an excipient, which is added in a weight ratio of 1 to 5;

1-6 parts of a composite antioxidant, wherein the weight part ratio of the composite antioxidant is 1-6 parts;

a buffering agent, which is added by weight part ratio of 25-45 parts.

According to the scheme, the barley, the alfalfa, the pea and the sesame are mixed to extract the plant antibacterial peptide, the prepared antibacterial peptide is bioactive small molecular peptide, the water solubility is good, the stable plant natural polypeptide structure and the animal natural antibacterial peptide have a synergistic effect, the more excellent antibacterial performance can be shown, the interference of an additive on a bacterial efflux mechanism is increased, and the remarkable anti-inflammatory and skin-calming effects are achieved; the plant antibacterial peptide, the animal antibacterial peptide and the phospholipid of the anionic liposome jointly act on the propionibacterium acnes, so that the tolerance of the propionibacterium acnes is reduced, and the bactericidal effect is further promoted, so that the minimum bacteriostatic concentration of the acne-removing additive is greatly reduced, the antibacterial activity of the acne-removing additive is effectively improved on the premise of lower production cost, and the acne-removing effect is ensured. In addition, the plant antibacterial peptide, the animal antibacterial peptide, the stabilizer, the excipient, the composite antioxidant and the buffering agent are compounded, so that the environmental resistance of the acne removing additive is improved, and the prepared acne removing additive has more stable preservation quality.

Preferably, the barley, the alfalfa, the pea and the sesame are mixed according to the weight ratio of 1:1-5:2-7: 1-4.

Preferably, the animal antibacterial peptide is obtained by extracting artificial bred silkworms with 1, 2-propanediol.

Preferably, the phospholipid of the anionic liposome is one or more of phosphatidic acid, phosphatidylglycerol, phosphatidylserine and phosphatidylinositol.

Preferably, the stabilizer is one or more of tween-80, Pluronic F68, Pluronic F88.

Preferably, the pH adjusting agent is citric acid.

Preferably, the excipient is one or more of mannitol, sorbitol, trehalose, chitosan.

Preferably, the preparation method of the acne-removing additive comprises the following steps: mixing the phospholipid of the anionic liposome, the excipient and the buffering agent in advance according to the weight part ratio, adding the plant antibacterial peptide, the animal antibacterial peptide, the stabilizing agent and the composite antioxidant under the stirring condition, uniformly dispersing, adjusting the pH to 6.5-7.0 by using a pH regulator, standing for 5-10 h, and carrying out vacuum freeze drying.

In addition, the invention also provides application of the acne-removing additive in preparation of cosmetics.

Preferably, the cosmetic is one of cream-like cosmetics, emulsion-like cosmetics and water-like cosmetics.

The acne-removing additive is applied to cosmetics, has remarkable effects of killing acne bacilli and staphylococcus aureus, has the effects of diminishing inflammation and calming the skin, prevents and reduces skin infection, promotes the function of repairing skin barriers, reduces skin lipid oxidation and reduces the recurrence probability of acne.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to embodiments thereof. It should be understood that the detailed description and specific examples, while indicating the scope of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

In an embodiment of the present invention, an acne treatment additive comprises:

the plant antibacterial peptide is obtained by extracting a mixture of barley, alfalfa, peas and sesame and then carrying out enzymatic hydrolysis by using a complex enzyme, wherein the added weight portion ratio of the plant antibacterial peptide is 15-30 portions;

an animal antibacterial peptide, which is added by weight portion of 8-12 portions;

the phospholipid of anionic liposome is added in a weight ratio of 10 to 13;

a stabilizer, which is added in a weight ratio of 20 to 35;

a pH regulator, which is added in a weight ratio of 0.1 to 3;

an excipient, which is added in a weight ratio of 1 to 5;

1-6 parts of a composite antioxidant, wherein the weight part ratio of the composite antioxidant is 1-6 parts;

a buffering agent, which is added by weight part ratio of 25-45 parts.

According to the scheme, the barley, the alfalfa, the pea and the sesame are mixed to extract the plant antibacterial peptide, the prepared antibacterial peptide is bioactive small molecular peptide, the water solubility is good, the stable plant natural polypeptide structure and the animal natural antibacterial peptide have a synergistic effect, the more excellent antibacterial performance can be shown, the interference of an additive on a bacterial efflux mechanism is increased, and the remarkable anti-inflammatory and skin-calming effects are achieved; the plant antibacterial peptide, the animal antibacterial peptide and the phospholipid of the anionic liposome jointly act on the propionibacterium acnes, so that the tolerance of the propionibacterium acnes is reduced, and the bactericidal effect is further promoted, so that the minimum bacteriostatic concentration of the acne-removing additive is greatly reduced, the antibacterial activity of the acne-removing additive is effectively improved on the premise of lower production cost, and the acne-removing effect is ensured. In addition, the plant antibacterial peptide, the animal antibacterial peptide, the stabilizer, the excipient, the composite antioxidant and the buffering agent are compounded, so that the environmental resistance of the acne removing additive is improved, and the prepared acne removing additive has more stable preservation quality.

In one embodiment, the barley, the alfalfa, the pea and the sesame are mixed in a weight ratio of 1:1-5:2-7: 1-4.

In one embodiment, the animal antibacterial peptide is obtained by extracting artificial bred silkworms with 1, 2-propanediol.

In one embodiment, the phospholipid of the anionic liposome is one or more of phosphatidic acid, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol.

In one embodiment, the stabilizer is one or more of tween-80, Pluronic F68, Pluronic F88.

In one embodiment, the pH adjusting agent is citric acid.

In one embodiment, the excipient is one or more of mannitol, sorbitol, trehalose, chitosan.

In one embodiment, the composite antioxidant is obtained by mixing tea polyphenol, ascorbyl palmitate and rosemary extract according to the mass part ratio of 1-3:1-5: 0.1-0.7. The added composite antioxidant is a natural composite antioxidant, has the advantages of safety and mildness, and has excellent antioxidation when being used on skin to reduce the damage of the environment to the skin while increasing the environmental resistance of the acne removing additive by synergistic addition.

In one embodiment, the buffer is sodium alginate at a concentration of 0.3mg/mL to 0.6mg/mL and polylysine at a concentration of 0.2mg/mL to 0.5 mg/mL.

In one embodiment, the mass part ratio of the sodium alginate to the polylysine is 1: 1. The sodium alginate is an anionic electrolyte, the polylysine is a cationic electrolyte, and the sodium alginate and the polylysine are compounded to serve as a buffering agent which can resist external effects, so that the acne removing additive is not easy to deteriorate in the medium, and when the acne removing additive is applied to cosmetics, the skin is not easy to be irritated, and the acne removing additive also has good adhesive force.

In one embodiment, the complex enzyme is prepared by mixing papain, cellulase and mannanase according to the weight ratio of 1-10:5-9: 0.2-1.8.

In one embodiment, the preparation method of the acne-removing additive comprises the following steps: mixing the phospholipid of the anionic liposome, the excipient and the buffering agent in advance according to the weight part ratio, adding the plant antibacterial peptide, the animal antibacterial peptide, the stabilizing agent and the composite antioxidant under the stirring condition, uniformly dispersing, adjusting the pH to 6.5-7.0 by using a pH regulator, standing for 5-10 h, and carrying out vacuum freeze drying. The powder is formed after vacuum freeze drying, which is more beneficial to storage and transportation and increases the convenience of application.

In addition, the invention also provides application of the acne-removing additive in preparation of cosmetics.

In one embodiment, the cosmetic is one of cream-like cosmetic, emulsion-like cosmetic, and water-like cosmetic.

The acne-removing additive is applied to cosmetics, has remarkable effects of killing acne bacilli and staphylococcus aureus, has the effects of diminishing inflammation and calming the skin, prevents and reduces skin infection, promotes the function of repairing skin barriers, reduces skin lipid oxidation and reduces the recurrence probability of acne.

In one embodiment, the acne-removing cream comprises the following components in percentage by mass: 0.1-10% of acne-removing additive, 20-35% of hyaluronic acid, 1.2-9% of trisodium 6-phosphate, 1.6-13% of glycerol, 0.01-0.03% of carbazone and 12-20% of dodecyl trimethyl ammonium chloride.

The acne-removing additive is combined with hyaluronic acid and glycerin in the acne-removing cream, has an excellent function of repairing skin barrier function, has remarkable functions of inhibiting bacteria, diminishing inflammation, calming skin and improving skin immunity, and also has the functions of preventing ultraviolet rays, removing free radicals and improving skin elasticity.

In one embodiment, an acne removing lotion is provided, which comprises the following components in percentage by mass: 0.1-8% of acne-removing additive, 0.1-0.3% of ginseng extract, 10-20% of lecithin, 2-12% of olive oil, 1-3% of squalane, 1-5% of propylene glycol, 10-20% of sodium hyaluronate, 0.01-0.03% of citric acid, 0.001-0.003% of carbomer, 0.1-0.3% of sodium chloride, 1-8% of cetearyl caprylate, 2-4% of isononyl isononanoate, 1-8% of isooctyl palmitate, 2-7% of caprylic/capric triglyceride and 0.001-0.003% of phenoxyethanol.

The acne-removing additive has high stability in the acne-removing emulsion, has excellent compounding performance with other components, and can effectively play the roles of bacteriostasis, inflammation diminishing and skin calming.

In one embodiment, the acne-removing toner is provided and comprises the following components in percentage by mass: 0.5-1.8% of acne-removing additive, 0.1-0.5% of tea tree extract, 0.8-1.2% of chamomile extract, 1-3% of polygonatum extract, 1-3% of aloe extract, 0.01-0.05% of dipotassium glycyrrhizinate and 0.01-0.05% of polysorbate.

The acne-removing additive is applied to the preparation of the acne-removing toner, and has a synergistic effect with the tea tree extract, the chamomile extract, the polygonatum odoratum extract and the aloe extract, so that the acne-removing toner has an excellent moisturizing effect, can obviously improve inflammatory skin, and has no stimulation to the skin.

Embodiments of the present invention will be described in detail below with reference to specific examples.

Example 1

The preparation method of the acne-removing additive comprises the following steps:

mixing papain, cellulase and mannase in a weight ratio of 1:5:1.8 to obtain a complex enzyme; mixing the barley, the alfalfa, the peas and the sesame in a weight ratio of 1:1:2:1 to obtain a mixture, wherein the weight ratio of the feed liquid is 1: adding acetic acid into the mixture, extracting the mixture for 30min under the power of 150W, adding complex enzyme with the weight being 1% of the mixture for enzymolysis for 20min to obtain plant antibacterial peptide;

adding 1, 2-propylene glycol into the artificially cultured silkworms killed in advance according to the weight ratio of 1:10 of the feed liquid to extract the animal antibacterial peptide;

mixing tea polyphenol, ascorbyl palmitate and rosemary extract according to the mass ratio of 1:5:0.7 to obtain a composite antioxidant;

according to the weight portion ratio of 1:1, mixing sodium alginate with the concentration of 0.3mg/mL and polylysine with the concentration of 0.5mg/mL to obtain a buffering agent;

13 parts of phosphatidic acid, 5 parts of mannitol and 25 parts of buffering agent are mixed in advance according to the weight part ratio, then 15 parts of plant antibacterial peptide, 8 parts of animal antibacterial peptide, 8035 parts of tween and 6 parts of compound antioxidant are added under the condition of stirring, after uniform dispersion, 3 parts of citric acid is used for adjusting the pH value to 6.5-7.0, standing is carried out for 5 hours, and vacuum freeze drying is carried out, thus obtaining the acne-removing additive.

Example 2:

the preparation method of the acne-removing additive comprises the following steps:

mixing papain, cellulase and mannase in a weight ratio of 10:5:1.8 to obtain a complex enzyme; mixing the barley, the alfalfa, the peas and the sesame in a weight ratio of 1:5:7:4 to obtain a mixture, wherein the weight ratio of the feed liquid is 1: adding acetic acid into the mixture, extracting the mixture for 30min under the power of 150W, adding complex enzyme with the weight being 2% of the mixture for enzymolysis for 20min to obtain plant antibacterial peptide;

adding 1, 2-propylene glycol into the artificially cultured silkworms killed in advance according to the weight ratio of 1:10 of the feed liquid to extract the animal antibacterial peptide;

mixing tea polyphenol, ascorbyl palmitate and rosemary extract according to the mass ratio of 3:1:0.7 to obtain a composite antioxidant;

according to the weight portion ratio of 1:1, mixing sodium alginate with the concentration of 0.6mg/mL and polylysine with the concentration of 0.2mg/mL to obtain a buffering agent;

13 parts of phosphatidyl glycerol, 1 part of sorbitol and 45 parts of buffering agent are mixed in advance according to the weight part ratio, 30 parts of plant antibacterial peptide, 12 parts of animal antibacterial peptide, 6835 parts of Pluronic F and 6 parts of composite antioxidant are added under the stirring condition, after uniform dispersion, 3 parts of citric acid is used for adjusting the pH value to 6.5-7.0, standing is carried out for 10h, and vacuum freeze drying is carried out, thus obtaining the acne-removing additive.

Example 3:

the preparation method of the acne-removing additive comprises the following steps:

mixing papain, cellulase and mannase in a weight ratio of 5:6:1.1 to obtain a complex enzyme; mixing the barley, the alfalfa, the peas and the sesame in a weight ratio of 1:3:4:2 to obtain a mixture, wherein the mixture comprises the following components in a material-liquid ratio of 1: adding acetic acid into the mixture, extracting the mixture for 30min under the power of 150W, adding compound enzyme with the weight being 3% of the mixture for enzymolysis for 20min to obtain plant antibacterial peptide;

adding 1, 2-propylene glycol into the artificially cultured silkworms killed in advance according to the weight ratio of 1:10 of the feed liquid to extract the animal antibacterial peptide;

mixing tea polyphenol, ascorbyl palmitate and rosemary extract according to the mass ratio of 2:5:0.6 to obtain a composite antioxidant;

according to the weight portion ratio of 1:1, mixing sodium alginate with the concentration of 0.4mg/mL and polylysine with the concentration of 0.4mg/mL to obtain a buffering agent;

mixing 11 parts of phosphatidylinositol, 3 parts of trehalose and 30 parts of a buffering agent in advance according to the weight part ratio, adding 20 parts of plant antibacterial peptide, 10 parts of animal antibacterial peptide, 8025 parts of tween-80 and 2 parts of compound antioxidant under the stirring condition, uniformly dispersing, adjusting the pH to 7.0 by using 0.2 part of citric acid, standing for 8 hours, and carrying out vacuum freeze drying to obtain the acne-removing additive.

Comparative example 1:

the preparation method of the acne-removing additive comprises the following steps:

adding 1, 2-propylene glycol into artificially cultured silkworms killed in advance at a ratio of the reference solution to 1:10 to extract animal antibacterial peptide;

mixing tea polyphenol, ascorbyl palmitate and rosemary extract according to the mass ratio of 2:5:0.6 to obtain a composite antioxidant;

according to the weight portion ratio of 1:1, mixing sodium alginate with the concentration of 0.4mg/mL and polylysine with the concentration of 0.4mg/mL to obtain a buffering agent;

pre-mixing 11 parts of phosphatidylinositol, 3 parts of trehalose and 30 parts of buffering agent according to the weight part ratio, adding 20 parts of animal antibacterial peptide, 8025 parts of tween and 2 parts of compound antioxidant under the stirring condition, uniformly dispersing, adjusting the pH to 7.0 by using 0.2 part of citric acid, standing for 8 hours, and carrying out vacuum freeze drying to obtain the acne-removing additive.

Comparative example 2:

the preparation method of the acne-removing additive comprises the following steps:

mixing papain, cellulase and mannase in a weight ratio of 5:6:1.1 to obtain a complex enzyme; mixing barley, alfalfa, peas and sesame in a weight ratio of 1:3:4:2, wherein the weight ratio of the barley to the alfalfa to the peas to the sesame is 1: adding acetic acid into the mixture, extracting the mixture for 30min under the power of 150W, adding compound enzyme with the weight being 3% of the mixture for enzymolysis for 20min to obtain plant antibacterial peptide;

adding 1, 2-propylene glycol into the artificially cultured silkworms killed in advance according to the ratio of 1:10 to extract animal antibacterial peptide;

and mixing 20 parts of plant antibacterial peptide and 10 parts of animal antibacterial peptide according to parts by weight, and carrying out vacuum freeze drying to obtain the acne-removing additive.

Comparative example 3:

the preparation method of the acne-removing additive comprises the following steps:

mixing papain, cellulase and mannase in a weight ratio of 5:6:1.1 to obtain a complex enzyme; mixing the barley, the alfalfa, the peas and the sesame in a weight ratio of 1:3:4:2 to obtain a mixture, wherein the mixture comprises the following components in a material-liquid ratio of 1: adding acetic acid into the mixture, extracting the mixture for 30min under the power of 150W, adding compound enzyme with the weight being 3% of the mixture for enzymolysis for 20min to obtain plant antibacterial peptide;

adding 1, 2-propylene glycol into the artificially cultured silkworms killed in advance according to the weight ratio of 1:10 of the feed liquid to extract the animal antibacterial peptide;

mixing tea polyphenol, ascorbyl palmitate and rosemary extract according to the mass ratio of 2:5:0.6 to obtain a composite antioxidant;

mixing 11 parts of phosphatidylinositol and 3 parts of trehalose in parts by weight in advance, adding 20 parts of plant antibacterial peptide, 10 parts of animal antibacterial peptide, 8025 parts of tween-80 and 2 parts of compound antioxidant under the condition of stirring, uniformly dispersing, adjusting the pH to 7.0 by using 0.2 part of citric acid, standing for 8 hours, and carrying out vacuum freeze drying to obtain the acne-removing additive.

Test example 1: bacteriostasis test

Distilled water is added into the acne removing additive prepared in the example 1-3 and the acne removing additive prepared in the comparative example 1-3 according to the volume ratio of 1:8, and 1mL of the distilled water is taken as a test solution respectively.

The determination method comprises the following steps: antibacterial activity determination is performed by adopting filter paper determination method, 0.5ml of bacterial suspension containing propionibacterium acnes is inoculated on an NA plate by using a coating method, filter paper sheets of respectively soaked determination test solutions are taken by using a sterilization forceps and are flatly placed in a culture dish containing test bacteria, and each group is repeated for 3 times. 0.5ml of a bacterial suspension of staphylococcus aureus is inoculated on an NA flat plate by a coating method, filter paper sheets of respectively soaked determination test solutions are taken by a pair of sterilized tweezers and are horizontally placed in a culture dish containing test bacteria, each group is repeated for 3 times, and sterile water is used as a blank control. The prepared culture dish is placed in a constant temperature incubator at 30 ℃, observed after 48 hours, and the diameter of the inhibition zone is measured by a cross method. The results of the zone diameter measurements are shown in table 1 below.

Table 1:

as can be seen from the analysis of the bacteriostatic test in table 1, the acne-removing additives prepared in examples 1 to 3 have excellent bacteriostatic effect, and the plant antimicrobial peptide added in comparative example 1 has a poorer antibacterial effect than the acne-removing additives prepared in examples 1 to 3; in the comparative example 2, the plant antibacterial peptide and the animal antibacterial peptide are directly mixed and freeze-dried, still have higher antibacterial effect, but are not subjected to the synergistic antagonistic environment of other components, and certain environmental influence exists in the test process; comparative example 3, in which no buffering agent is added but the antibacterial effect is influenced, illustrates the preparation of the anti-acne additive in examples 1-3, and the presence of the buffering agent, the stabilizing agent, the composite antioxidant, the excipient and the phospholipid of the anionic liposome helps to ensure the bacteriostatic activity of the antibacterial peptide.

Application example 1:

the acne-removing facial cream comprises the following components in percentage by mass: acne removing additive 0.1% prepared in example 1, hyaluronic acid 35%, trisodium 6-phosphate glucose 1.2%, glycerin 1.6%, cason 0.01%, dodecyl trimethyl ammonium chloride 12%. The acne-removing face cream is prepared by a conventional method.

The acne-removing additive is combined with hyaluronic acid and glycerin in the acne-removing cream, has an excellent function of repairing skin barrier function, has remarkable functions of inhibiting bacteria, diminishing inflammation, calming skin and improving skin immunity, and also has the functions of preventing ultraviolet rays, removing free radicals and improving skin elasticity.

Application example 2:

an acne-removing emulsion comprises the following components in percentage by mass: the acne treatment additive prepared in example 2 was 8%, ginseng extract 0.3%, lecithin 10%, olive oil 2%, squalane 3%, propylene glycol 1%, sodium hyaluronate 20%, citric acid 0.03%, carbomer 0.001%, sodium chloride 0.1%, cetearyl caprylate 1%, isononyl isononanoate 4%, isooctyl palmitate 8%, caprylic/capric triglyceride 7%, phenoxyethanol 0.003%. The acne removing lotion is prepared by a conventional method.

The acne-removing additive has high stability in the acne-removing emulsion, has excellent compounding performance with other components, and can effectively play the roles of bacteriostasis, inflammation diminishing and skin calming.

Application example 3:

the acne-removing toner comprises the following components in percentage by mass: the acne treatment additive prepared in example 3 is 1.8%, tea tree extract 0.1%, chamomile extract 0.8%, polygonatum odoratum extract 3%, aloe extract 3%, dipotassium glycyrrhizinate 0.01%, and polysorbate 0.05%. The acne-removing toner is prepared by a conventional method.

The acne-removing additive is applied to the preparation of the acne-removing toner, and has a synergistic effect with the tea tree extract, the chamomile extract, the polygonatum odoratum extract and the aloe extract, so that the acne-removing toner has an excellent moisturizing effect, can obviously improve inflammatory skin, and has no stimulation to the skin.

Test example 2:

at least 20 volunteers with face being acne skin are screened in each group, and 3 groups are provided in total, wherein the acne removing cream prepared in the first group of test application example 1, the acne removing lotion prepared in the second group of test application example 2 and the acne removing toner prepared in the third group of test application example 3 are adopted. And the screened volunteers also have inflammation, redness and the like on the face, the age range of the crowd is 18-45 years, products in the application example are not smeared on one side face to serve as blank controls (hereinafter referred to as control groups), products in the smearing application example serve as sample groups, the blank controls, the control groups and the sample groups are tested on 0 day, 7 day and 14 day respectively, and the moisture content index of the skin is measured to evaluate the skin repairing capacity and the continuous water replenishing capacity of the cosmetics. Skin moisture content is shown in table 2.

Table 2:

as can be seen from the data analysis in Table 2, the water content of the skin is obviously increased by using the product in the application example, and a good skin ecological environment can be created for skin barrier repair.

In addition, as is clear from the evaluation of use in test example 2, 96% of volunteers showed that the cosmetic composition of the test example had significant anti-inflammatory and sedative effects on the skin, significantly improved redness of the skin, significantly improved acne skin with a low recurrence rate, and had excellent skin feel in use. 4% of volunteers showed that the acne-removing product had the effect of improving the skin of acne-removing skin; when the acne removing product is used, the elasticity and the whiteness of the skin are also increased.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.