阅读说明：本技术 一种自体脂肪源干细胞细胞因子提取液的眼霜制备方法 (Preparation method of eye cream of autologous adipose-derived stem cell cytokine extracting solution ) 是由 占震锋 李庆静 于 2021-01-07 设计创作，主要内容包括：本发明公开了一种自体脂肪源干细胞细胞因子提取液的眼霜制备方法,该面霜包括如下重量份原料：自体脂肪源干细胞细胞因子提取液1-3份、美白凝胶5-8份、甘油5-10份、氨甲基丙醇0.2-0.5份、十八醇1-2份、乙酰化六肽0.5-1份、去离子水50-80份、角鲨烷1-5份、橄榄油10-15份、鲸蜡醇3-5份、肌氨酸钠微1-1.5份、异硬脂醇新戊酸酯0.5-1份、透明质酸钠0.05-0.2份、失水山梨醇单油酸酯0.5-1份；将琼脂糖进行酶解,得到琼寡糖溶液,将混合提取膏和卡姆波混合,制得美白凝胶,该美白凝胶能够抑制酪氨酸酶,清除氧自由基的方式抑制黑色素生成,进而达到美白效果。(The invention discloses a preparation method of eye cream of an autologous adipose-derived stem cell cytokine extracting solution, wherein the eye cream comprises the following raw materials in parts by weight: 1-3 parts of autologous fat-derived stem cell cytokine extracting solution, 5-8 parts of whitening gel, 5-10 parts of glycerol, 0.2-0.5 part of aminomethyl propanol, 1-2 parts of octadecanol, 0.5-1 part of acetylated hexapeptide, 50-80 parts of deionized water, 1-5 parts of squalane, 10-15 parts of olive oil, 3-5 parts of cetyl alcohol, 1-1.5 parts of sodium sarcosinate, 0.5-1 part of isostearyl pivalate, 0.05-0.2 part of sodium hyaluronate and 0.5-1 part of sorbitan monooleate; carrying out enzymolysis on agarose to obtain an agar-agar oligosaccharide solution, and mixing the mixed extract with Carbam to prepare the whitening gel, wherein the whitening gel can inhibit tyrosinase and inhibit melanin generation in a manner of removing oxygen free radicals, thereby achieving the whitening effect.)

1. A preparation method of eye cream of autologous adipose-derived stem cell cytokine extract is characterized by comprising the following steps: the face cream comprises the following raw materials in parts by weight: 1-3 parts of autologous fat-derived stem cell cytokine extracting solution, 5-8 parts of whitening gel, 5-10 parts of glycerol, 0.2-0.5 part of aminomethyl propanol, 1-2 parts of octadecanol, 0.5-1 part of acetylated hexapeptide, 50-80 parts of deionized water, 1-5 parts of squalane, 10-15 parts of olive oil, 3-5 parts of cetyl alcohol, 1-1.5 parts of sodium sarcosinate, 0.5-1 part of isostearyl pivalate, 0.05-0.2 part of sodium hyaluronate and 0.5-1 part of sorbitan monooleate;

the preparation method of the eye cream specifically comprises the following steps:

step S1: adding the autologous adipose-derived stem cell cytokine extracting solution, whitening gel, glycerol, aminomethyl propanol, octadecanol, acetylated hexapeptide and deionized water into a stirring kettle, and stirring for 1-1.5h at the rotation speed of 500-800r/min and the temperature of 30-50 ℃ to prepare a first mixture;

step S2: adding squalane, olive oil, cetyl alcohol, sodium sarcosinate, isostearyl alcohol pivalate and sodium hyaluronate into a stirring kettle, and continuously stirring for 1-1.5h under the conditions that the rotating speed is 800-;

step S3: adding the first mixture and the second mixture into a stirring kettle, stirring for 3-5min at a rotating speed of 3000r/min and a temperature of 70-80 ℃, cooling to 40-50 ℃, adding sorbitan monooleate, continuously stirring for 1-1.5h, and cooling to room temperature to obtain the eye cream.

2. The method for preparing an eye cream from an autologous adipose-derived stem cell cytokine extract as claimed in claim 1, wherein the method comprises the steps of: the autologous adipose-derived stem cell factor extracting solution is prepared by the following steps:

step A1: adding normal saline into autologous fat liquid, standing at 10 deg.C for 5min for layering, discharging bottom liquid, centrifuging the rest liquid at 400r/min for 3min, and collecting bottom swelling liquid to obtain fat granules;

step A2: the fat particle chyle is crushed and then mixed with normal saline and gentamicin, after centrifugation is carried out for 5min at the temperature of 5 ℃ and the rotating speed of 2500r/min, four layers of layers are layered, top layer grease and third layer normal saline are removed, and a substrate, gentamicin and lidocaine are uniformly mixed to prepare the autologous adipose-derived stem cell cytokine extracting solution.

3. The method for preparing an eye cream from an autologous adipose-derived stem cell cytokine extract as claimed in claim 2, wherein the method comprises the steps of: the dosage volume ratio of the fat particles, the physiological saline and the gentamicin in the step A2 is 25:23:2, the dosage of the gentamicin is 4U added into each 15mL of the substrate, and the dosage volume ratio of the lidocaine and the substrate is 1: 5.

4. The method for preparing an eye cream from an autologous adipose-derived stem cell cytokine extract as claimed in claim 1, wherein the method comprises the steps of: the whitening gel is prepared by the following steps:

step B1: pulverizing and mixing Saururi herba, radix Ophiopogonis, radix astragali, rhizoma Ligustici Chuanxiong, herba Leonuri, and radix Paeoniae alba to obtain a mixture, adding the mixture and ethanol into a stirring kettle, refluxing for 3 times at a rotation speed of 150-;

step B2: adding the substrate obtained in the step B1 into deionized water, refluxing for 2 times at the temperature of 110-120 ℃, centrifuging for 10-15min at the rotating speed of 3000r/min after 1-1.5h each time, taking supernate to obtain a water extracting solution, respectively concentrating the water extracting solution and the alcohol extracting solution until the relative density is 1.5g/mL, and mixing to obtain a mixed extracting paste;

step B3: adding agarose and deionized water into a reaction kettle, stirring until the agarose is completely dissolved under the conditions of the rotation speed of 150-.

5. The method for preparing an eye cream from an autologous adipose-derived stem cell cytokine extract as claimed in claim 4, wherein the method comprises: the mass ratio of the three hundred grass, the radix ophiopogonis, the astragalus membranaceus, the ligusticum wallichii, the leonurus japonicus and the radix paeoniae alba in the step B1 is 1:1:1:1:1:1, the mass ratio of the mixture to the ethanol is 1g:15mL, the mass ratio of the substrate to the deionized water in the step B2 is 1g:15mL, the mass ratio of the agarose and the deionized water in the step B3 is 0.3g:100mL, the mass ratio of the agarase is 1U/mL, and the mass ratio of the supernatant, the mixed extraction paste and the carbomer 941 is 10mL:5g:3 g.

Technical Field

The invention relates to the technical field of eye cream preparation, and particularly relates to a preparation method of an autologous adipose-derived stem cell cytokine extracting solution.

Background

Autologous stem cells are "seed cells". The animal body realizes the cell renewal through the division of the autologous stem cells, thereby ensuring the continuous growth and development of the animal body. When a disease, an organ is struck or injured, autologous stem cells respond first time, promoting differentiation into the exclusive cells needed by the body, thereby providing sufficient numbers of exclusive cells to aid in repair and healing. Has wide application in the treatment of various diseases such as nervous system diseases, immune system diseases and the like. And europe has applied this technology to anti-aging and early prevention of disease.

After the existing eye cream is used for a period of time, eye bags can be eliminated to a certain degree, partial edema still exists, the removing effect of black eye circles is not obvious, and partial black color remains on eyes after the eye cream is used for a period of time.

Disclosure of Invention

The invention aims to provide a method for preparing eye cream from an autologous adipose-derived stem cell cytokine extracting solution.

The technical problems to be solved by the invention are as follows:

after the existing eye cream is used for a period of time, eye bags can be eliminated to a certain degree, partial edema still exists, the removing effect of black eye circles is not obvious, and partial black color remains on eyes after the eye cream is used for a period of time.

The purpose of the invention can be realized by the following technical scheme:

a preparation method of an eye cream of an autologous adipose-derived stem cell cytokine extracting solution comprises the following raw materials in parts by weight: 1-3 parts of autologous fat-derived stem cell cytokine extracting solution, 5-8 parts of whitening gel, 5-10 parts of glycerol, 0.2-0.5 part of aminomethyl propanol, 1-2 parts of octadecanol, 0.5-1 part of acetylated hexapeptide, 50-80 parts of deionized water, 1-5 parts of squalane, 10-15 parts of olive oil, 3-5 parts of cetyl alcohol, 1-1.5 parts of sodium sarcosinate, 0.5-1 part of isostearyl pivalate, 0.05-0.2 part of sodium hyaluronate and 0.5-1 part of sorbitan monooleate;

the preparation method of the eye cream specifically comprises the following steps:

step S1: adding the autologous adipose-derived stem cell cytokine extracting solution, whitening gel, glycerol, aminomethyl propanol, octadecanol, acetylated hexapeptide and deionized water into a stirring kettle, and stirring for 1-1.5h at the rotation speed of 500-800r/min and the temperature of 30-50 ℃ to prepare a first mixture;

step S2: adding squalane, olive oil, cetyl alcohol, sodium sarcosinate, isostearyl alcohol pivalate and sodium hyaluronate into a stirring kettle, and continuously stirring for 1-1.5h under the conditions that the rotating speed is 800-;

step S3: adding the first mixture and the second mixture into a stirring kettle, stirring for 3-5min at a rotating speed of 3000r/min and a temperature of 70-80 ℃, cooling to 40-50 ℃, adding sorbitan monooleate, continuously stirring for 1-1.5h, and cooling to room temperature to obtain the eye cream.

Further, the autologous adipose-derived stem cell factor extracting solution is prepared by the following steps:

step A1: adding normal saline into autologous fat liquid, standing at 10 deg.C for 5min for layering, discharging bottom liquid, centrifuging the rest liquid at 400r/min for 3min, and collecting bottom swelling liquid to obtain fat granules;

step A2: the fat particle chyle is crushed and then mixed with normal saline and gentamicin, after centrifugation is carried out for 5min at the temperature of 5 ℃ and the rotating speed of 2500r/min, four layers of layers are layered, top layer grease and third layer normal saline are removed, and a substrate, gentamicin and lidocaine are uniformly mixed to prepare the autologous adipose-derived stem cell cytokine extracting solution.

Further, the dosage volume ratio of the fat particles, the physiological saline and the gentamicin in the step A2 is 25:23:2, the dosage of the gentamicin is 4U per 15mL of the substrate, and the dosage volume ratio of the lidocaine and the substrate is 1: 5.

Further, the whitening gel is prepared by the following steps:

step B1: pulverizing and mixing Saururi herba, radix Ophiopogonis, radix astragali, rhizoma Ligustici Chuanxiong, herba Leonuri, and radix Paeoniae alba to obtain a mixture, adding the mixture and ethanol into a stirring kettle, refluxing for 3 times at a rotation speed of 150-;

step B2: adding the substrate obtained in the step B1 into deionized water, refluxing for 2 times at the temperature of 110-120 ℃, centrifuging for 10-15min at the rotating speed of 3000r/min after 1-1.5h each time, taking supernate to obtain a water extracting solution, respectively concentrating the water extracting solution and the alcohol extracting solution until the relative density is 1.5g/mL, and mixing to obtain a mixed extracting paste;

step B3: adding agarose and deionized water into a reaction kettle, stirring until the agarose is completely dissolved under the conditions of the rotation speed of 150-.

Further, the mass ratio of the three hundred grass, the radix ophiopogonis, the astragalus membranaceus, the ligusticum wallichii, the leonurus japonicus and the radix paeoniae alba in the step B1 is 1:1:1:1:1, the mass ratio of the mixture to the ethanol is 1g:15mL, the mass ratio of the substrate to the deionized water in the step B2 is 1g:15mL, the dosage of the agarose and the deionized water in the step B3 is 0.3g:100mL, the dosage of the agarase is 1U/mL, and the dosage ratio of the supernatant, the mixed extraction paste and the carbomer 941 is 10mL:5g:3 g.

The invention has the beneficial effects that: the invention extracts autologous adipose-derived stem cells in the process of preparing the facial cream of the autologous adipose-derived stem cell cytokine extracting solution, the autologous adipose-derived stem cell extract can form epidermal cells, bone and cartilage cells and stem cells and can differentiate to form myocardial cells, nerve cells, vascular endothelial cells and blood cells so as to regenerate tissues and achieve the effect of repairing skin, simultaneously prepares a whitening gel, the whitening gel takes Saururus chinensis, radix Ophiopogonis, radix astragali, rhizoma ligustici wallichii, herba leonuri and radix paeoniae alba as raw materials to carry out alcohol extraction to obtain alcohol extract, then re-extracts a substrate with deionized water to prepare water extract, concentrates and mixes the alcohol extract and the water extract to prepare extract, then carries out enzymolysis on agarose to obtain agar oligosaccharide solution, mixes the mixed extract and Carmu wave to prepare the whitening gel, the whitening gel can inhibit tyrosinase and inhibit melanin generation by scavenging oxygen free radicals, thereby achieving whitening effect.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

A preparation method of an eye cream of an autologous adipose-derived stem cell cytokine extracting solution comprises the following raw materials in parts by weight: 1 part of autologous fat-derived stem cell cytokine extracting solution, 5 parts of whitening gel, 5 parts of glycerol, 0.2 part of aminomethyl propanol, 1 part of octadecanol, 0.5 part of acetylated hexapeptide, 50 parts of deionized water, 1 part of squalane, 10 parts of olive oil, 3 parts of cetyl alcohol, 1 part of sodium sarcosinate, 0.5 part of isostearyl pivalate, 0.05 part of sodium hyaluronate and 0.5 part of sorbitan monooleate;

the preparation method of the eye cream specifically comprises the following steps:

step S1: adding the autologous adipose-derived stem cell cytokine extracting solution, whitening gel, glycerol, aminomethyl propanol, octadecanol, acetylated hexapeptide and deionized water into a stirring kettle, and stirring for 1h at the rotation speed of 500r/min and the temperature of 30 ℃ to prepare a first mixture;

step S2: adding squalane, olive oil, cetyl alcohol, sodium sarcosinate, isostearyl pivalate and sodium hyaluronate into a stirring kettle, and continuously stirring for 1h at the rotation speed of 800r/min and the temperature of 75 ℃ to prepare a second mixture;

step S3: adding the first mixture and the second mixture into a stirring kettle, stirring for 3min at the rotating speed of 3000r/min and the temperature of 70 ℃, cooling to the temperature of 40 ℃, adding sorbitan monooleate, continuously stirring for 1h, and cooling to room temperature to obtain the eye cream.

The autologous adipose-derived stem cell factor extracting solution is prepared by the following steps:

step A1: adding normal saline into autologous fat liquid, standing at 10 deg.C for 5min for layering, discharging bottom liquid, centrifuging the rest liquid at 400r/min for 3min, and collecting bottom swelling liquid to obtain fat granules;

step A2: the fat particle chyle is crushed and then mixed with normal saline and gentamicin, after centrifugation is carried out for 5min at the temperature of 5 ℃ and the rotating speed of 2500r/min, four layers of layers are layered, top layer grease and third layer normal saline are removed, and a substrate, gentamicin and lidocaine are uniformly mixed to prepare the autologous adipose-derived stem cell cytokine extracting solution.

The whitening gel is prepared by the following steps:

step B1: pulverizing Saururi herba, radix Ophiopogonis, radix astragali, rhizoma Ligustici Chuanxiong, herba Leonuri, and radix Paeoniae alba, mixing to obtain a mixture, adding the mixture and ethanol into a stirring kettle, refluxing at 150r/min and 80 deg.C for 3 times, centrifuging at 3000r/min for 10min after 2 hr each time, and collecting the substrate to obtain an ethanol extractive solution;

step B2: adding the substrate obtained in the step B1 into deionized water, refluxing for 2 times at the temperature of 110 ℃, centrifuging for 10min at the rotating speed of 3000r/min after 1h each time, taking supernate to obtain a water extracting solution, respectively concentrating the water extracting solution and the alcohol extracting solution to the relative density of 1.5g/mL, and mixing to obtain a mixed extracting paste;

step B3: adding agarose and deionized water into a reaction kettle, stirring until the agarose is completely dissolved under the conditions of the rotating speed of 150r/min and the temperature of 90 ℃, adding agarase, reacting for 10 hours at the temperature of 35 ℃, heating to the temperature of 110 ℃, preserving heat for 10 minutes, centrifuging for 10 minutes under the condition of the rotating speed of 3000r/min, taking supernatant, mixing the supernatant with mixed extraction paste, adding carbomer 941, and stirring for 1 hour under the condition of the rotating speed of 500r/min to obtain the whitening gel.

Example 2

A preparation method of an eye cream of an autologous adipose-derived stem cell cytokine extracting solution comprises the following raw materials in parts by weight: 2 parts of autologous fat-derived stem cell cytokine extracting solution, 6 parts of whitening gel, 8 parts of glycerol, 0.3 part of aminomethyl propanol, 1.5 parts of octadecanol, 0.8 part of acetylated hexapeptide, 60 parts of deionized water, 3 parts of squalane, 13 parts of olive oil, 4 parts of cetyl alcohol, 1.3 parts of sodium sarcosinate, 0.8 part of isostearyl pivalate, 0.15 part of sodium hyaluronate and 0.8 part of sorbitan monooleate;

the preparation method of the eye cream specifically comprises the following steps:

step S1: adding the autologous adipose-derived stem cell cytokine extracting solution, whitening gel, glycerol, aminomethyl propanol, octadecanol, acetylated hexapeptide and deionized water into a stirring kettle, and stirring for 1h at the rotation speed of 500r/min and the temperature of 50 ℃ to prepare a first mixture;

step S2: adding squalane, olive oil, cetyl alcohol, sodium sarcosinate, isostearyl pivalate and sodium hyaluronate into a stirring kettle, and continuously stirring for 1.5 hours at the rotation speed of 1000r/min and the temperature of 75 ℃ to prepare a second mixture;

step S3: adding the first mixture and the second mixture into a stirring kettle, stirring for 5min at the rotating speed of 3000r/min and the temperature of 70 ℃, cooling to the temperature of 40 ℃, adding sorbitan monooleate, continuously stirring for 1.5h, and cooling to room temperature to obtain the eye cream.

The autologous adipose-derived stem cell factor extracting solution is prepared by the following steps:

step A1: adding normal saline into autologous fat liquid, standing at 10 deg.C for 5min for layering, discharging bottom liquid, centrifuging the rest liquid at 400r/min for 3min, and collecting bottom swelling liquid to obtain fat granules;

step A2: the fat particle chyle is crushed and then mixed with normal saline and gentamicin, after centrifugation is carried out for 5min at the temperature of 5 ℃ and the rotating speed of 2500r/min, four layers of layers are layered, top layer grease and third layer normal saline are removed, and a substrate, gentamicin and lidocaine are uniformly mixed to prepare the autologous adipose-derived stem cell cytokine extracting solution.

The whitening gel is prepared by the following steps:

step B1: pulverizing Saururi herba, radix Ophiopogonis, radix astragali, rhizoma Ligustici Chuanxiong, herba Leonuri, and radix Paeoniae alba, mixing to obtain a mixture, adding the mixture and ethanol into a stirring kettle, refluxing at 90 deg.C for 3 times at 150r/min for 2 hr each time, centrifuging at 3000r/min for 10min, and collecting the substrate to obtain an ethanol extractive solution;

step B2: adding the substrate obtained in the step B1 into deionized water, refluxing for 2 times at 120 ℃, centrifuging for 15min at the rotating speed of 3000r/min after 1h each time, taking supernate to obtain water extract, respectively concentrating the water extract and the alcohol extract until the relative density is 1.5g/mL, and mixing to obtain mixed extract;

step B3: adding agarose and deionized water into a reaction kettle, stirring until the agarose is completely dissolved under the conditions of the rotating speed of 150r/min and the temperature of 95 ℃, adding agarase, reacting for 10 hours at the temperature of 35 ℃, heating to 120 ℃, preserving heat for 10 minutes, centrifuging for 15 minutes under the condition of the rotating speed of 3000r/min, taking supernatant, mixing the supernatant with mixed extraction paste, adding carbomer 941, and stirring for 1.5 hours under the condition of the rotating speed of 500r/min to obtain the whitening gel.

Example 3

A preparation method of an eye cream of an autologous adipose-derived stem cell cytokine extracting solution comprises the following raw materials in parts by weight: 3 parts of autologous fat-derived stem cell cytokine extracting solution, 8 parts of whitening gel, 10 parts of glycerol, 0.5 part of aminomethyl propanol, 2 parts of octadecanol, 1 part of acetylated hexapeptide, 80 parts of deionized water, 5 parts of squalane, 15 parts of olive oil, 5 parts of cetyl alcohol, 1.5 parts of sodium sarcosinate, 1 part of isostearyl pivalate, 0.2 part of sodium hyaluronate and 1 part of sorbitan monooleate;

the preparation method of the eye cream specifically comprises the following steps:

step S1: adding the autologous adipose-derived stem cell cytokine extracting solution, whitening gel, glycerol, aminomethyl propanol, octadecanol, acetylated hexapeptide and deionized water into a stirring kettle, and stirring for 1.5h at the rotation speed of 800r/min and the temperature of 50 ℃ to prepare a first mixture;

step S2: adding squalane, olive oil, cetyl alcohol, sodium sarcosinate, isostearyl pivalate and sodium hyaluronate into a stirring kettle, and continuously stirring for 1.5 hours at the rotation speed of 1000r/min and the temperature of 80 ℃ to prepare a second mixture;

step S3: adding the first mixture and the second mixture into a stirring kettle, stirring for 5min at a rotating speed of 3000r/min and a temperature of 80 ℃, cooling to a temperature of 50 ℃, adding sorbitan monooleate, continuously stirring for 1.5h, and cooling to room temperature to obtain the eye cream.

The autologous adipose-derived stem cell factor extracting solution is prepared by the following steps:

step A1: adding normal saline into autologous fat liquid, standing at 10 deg.C for 5min for layering, discharging bottom liquid, centrifuging the rest liquid at 400r/min for 3min, and collecting bottom swelling liquid to obtain fat granules;

step A2: the fat particle chyle is crushed and then mixed with normal saline and gentamicin, after centrifugation is carried out for 5min at the temperature of 5 ℃ and the rotating speed of 2500r/min, four layers of layers are layered, top layer grease and third layer normal saline are removed, and a substrate, gentamicin and lidocaine are uniformly mixed to prepare the autologous adipose-derived stem cell cytokine extracting solution.

The whitening gel is prepared by the following steps:

step B1: pulverizing Saururi herba, radix Ophiopogonis, radix astragali, rhizoma Ligustici Chuanxiong, herba Leonuri, and radix Paeoniae alba, mixing to obtain a mixture, adding the mixture and ethanol into a stirring kettle, refluxing at 90 deg.C at 200r/min for 3 times, centrifuging at 3000r/min for 15min after 2 hr each time, and collecting the substrate to obtain an ethanol extractive solution;

step B2: adding the substrate obtained in the step B1 into deionized water, refluxing for 2 times at 120 ℃, after 1.5h each time, centrifuging for 15min at the rotating speed of 3000r/min, taking supernatant to obtain water extract, concentrating the water extract and the alcohol extract respectively to the relative density of 1.5g/mL, and mixing to obtain mixed extract;

step B3: adding agarose and deionized water into a reaction kettle, stirring until the agarose is completely dissolved under the conditions of the rotating speed of 200r/min and the temperature of 95 ℃, adding agarase, reacting for 15 hours at the temperature of 35 ℃, heating to 120 ℃, keeping the temperature for 15 minutes, centrifuging for 15 minutes under the condition of the rotating speed of 3000r/min, taking supernatant, mixing the supernatant with mixed extraction paste, adding carbomer 941, and stirring for 1.5 hours under the condition of the rotating speed of 800r/min to obtain the whitening gel.

Comparative example

The comparative example is a common eye cream in the market.

Randomly selecting 200 male and female of 18-25 years old, dividing into four groups, respectively applying the face cream prepared in the examples 1-3 to the first group to the third group, applying the face cream prepared in the comparative example to the fourth group, cleaning the face every morning and evening, and continuously using for 30 days to observe under-eye bags and black eyes, as shown in the following table 1;

TABLE 1

	Example 1	Example 2	Example 3	Comparative example
					Eye bag	Good eliminating effect	Good eliminating effect	Good eliminating effect	Poor eliminating effect
Black eye	Complete elimination	Complete elimination	Complete elimination	There are still partial black eyes

As can be seen from table 1 above, the eye creams prepared in examples 1 to 3 have good removal effects on under-eye puffiness and black eyes, while the black eyes prepared in the comparative example have poor removal effects on under-eye puffiness and black eyes, and the invention has good under-eye puffiness and black eye removal effects.

The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.