Chitosan oligosaccharide preparation method and weight-losing tablets

文档序号：758737 发布日期：2021-04-06 浏览：17次中文

阅读说明：本技术 一种壳寡糖的制备方法及减肥片 (Chitosan oligosaccharide preparation method and weight-losing tablets ) 是由潘冬梅马韵升蔡颖辉张心青杨传伦田杰伟刘海玉刘结磊杨丹丹韩立霞于 2020-12-17 设计创作，主要内容包括：本发明提供了一种壳寡糖的制备方法,包括：S1)将壳聚糖在酸性水溶液中溶胀,然后加入壳聚糖酶,加热进行酶解,得到酶解液；S2)将所述酶解液经灭酶处理、澄清处理后,得到数均分子量≤1Kda的液体壳寡糖。与现有技术相比,本发明采用生物酶解法制备数均分子量≤1Kda的壳寡糖,酶解结束后进行了灭酶处理,没有引入别的物质,保证了产品的安全性,为制备天然类物质减肥产品提供了可靠原料。进一步采用天然糖类物质壳寡糖(脱乙酰度≥90％、数均分子量≤1KDa)制备减肥片,在不影响食欲、无腹泻的情况,具有增加脂质的排泄量、改善血脂紊乱、减少脂肪组织增生,达到减肥降脂的效果,是首个天然糖类减肥新原料。(The invention provides a preparation method of chitosan oligosaccharide, which comprises the following steps: s1) swelling chitosan in an acidic aqueous solution, adding chitosan enzyme, and heating for enzymolysis to obtain an enzymolysis solution; s2) carrying out enzyme deactivation treatment and clarification treatment on the enzymolysis liquid to obtain the liquid chitosan oligosaccharide with the number average molecular weight less than or equal to 1 Kda. Compared with the prior art, the invention adopts the biological enzymolysis method to prepare the chitosan oligosaccharide with the number average molecular weight less than or equal to 1Kda, carries out enzyme deactivation treatment after the enzymolysis is finished, does not introduce other substances, ensures the safety of the product, and provides reliable raw materials for preparing natural substance weight-reducing products. The weight-reducing tablet is further prepared from natural saccharide chitosan oligosaccharide (the deacetylation degree is more than or equal to 90 percent, and the number average molecular weight is less than or equal to 1KDa), has the effects of increasing the excretion of lipid, improving the blood lipid disorder, reducing the hyperplasia of adipose tissues and reducing weight and fat under the condition of not affecting appetite and having no diarrhea, and is the first new natural saccharide weight-reducing raw material.)

1. A method for preparing chitosan oligosaccharide is characterized by comprising the following steps:

s1) swelling chitosan in an acidic aqueous solution, adding chitosan enzyme, and heating for enzymolysis to obtain an enzymolysis solution;

s2) carrying out enzyme deactivation treatment and clarification treatment on the enzymolysis liquid to obtain the liquid chitosan oligosaccharide with the number average molecular weight less than or equal to 1 Kda.

2. The preparation method of claim 1, wherein the degree of deacetylation of chitosan is greater than or equal to 90%; the mass concentration of chitosan in the acidic aqueous solution is preferably 2-8%; the pH value of the acidic aqueous solution is 4.5-5.5.

3. The method according to claim 1, wherein the swelling temperature is 50 ℃ to 60 ℃; the swelling time is 3-5 h; the temperature of the enzymolysis is 50-60 ℃; the enzymolysis time is 3-5 h; the temperature of the enzyme deactivation treatment is 75-85 ℃; the enzyme deactivation treatment time is 10-20 min.

4. The preparation method according to claim 1, wherein the ratio of the chitosanase to the chitosan is (30-40) U: 1g of the total weight of the composition.

5. The preparation method according to claim 1, wherein the clarification treatment is filtration after adding a clarifying agent; the mass of the clarifying agent is 0.3-0.8% of that of the acidic aqueous solution; the clarifying agent is diatomite.

6. The method of claim 1, further comprising:

s3) concentrating the liquid chitosan oligosaccharide with the number average molecular weight of less than or equal to 1Kda under reduced pressure, and then spray drying to obtain the solid chitosan oligosaccharide with the number average molecular weight of less than or equal to 1 Kda.

7. The method according to claim 6, wherein the temperature of the reduced pressure concentration is 75 ℃ to 85 ℃; and carrying out reduced pressure concentration until the dry matter content is 15-30%.

8. The preparation method according to claim 6, wherein the inlet air temperature of the spray drying is 180-220 ℃, and the outlet air temperature is 75-85 ℃.

9. A weight-reducing tablet comprising the chitosan oligosaccharide prepared by the preparation method of any one of claims 1 to 8.

10. The slimming tablet of claim 9, comprising:

Technical Field

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a preparation method of chitosan oligosaccharide and a weight-losing tablet.

Background

Obesity is a chronic nutritional disease in which metabolic disorders occur due to excessive accumulation of body fat as a result of loss of balance in body energy intake and expenditure. With the increase of economic level and the improvement of living environment, the incidence rate of obesity continuously rises in the global scope, and obesity has become a great problem endangering the health of the public in society. According to the WHO statistical data in 2020, the prevalence of chemical fertilizer obesity in adults worldwide has increased 1.5 times since 2000, and in 2016, over one third of adults worldwide (18 years and older) are overweight, with obese people accounting for 13% of the global population. At the end of the nineteenth century, western countries began using weight loss drugs, but many weight loss drugs used for a long time have been released from the market due to problems such as severe toxic and side effects.

Orlistat (Orlistat) is currently the only OTC antiobesity agent worldwide. Orlistat is a strong and long-acting specific gastrointestinal lipase inhibitor, achieves the purpose of losing weight by directly blocking the absorption of fat in food by a human body, but also has the adverse reactions of frequent defecation, abdominal pain, nausea, vomiting, oily hiccup and the like. Therefore, a new weight-losing medicine with obvious curative effect, safety and no toxicity is urgently needed to better lose weight healthily.

Chitosan oligosaccharide is a degradation product of chitosan, and is widely applied to food, biomedicine and cosmetics.

The production of chitosan oligosaccharide is commonly carried out by physical degradation method, chemical degradation method and biological enzymolysis method. Wherein, the physical degradation method is usually an ultrasonic degradation method, chitosan oligosaccharide is prepared by degrading chitosan through ultrasonic waves, the reaction process is mild, but the yield of the water-soluble product with low molecular weight in the final product is low; the chemical degradation method is usually used for preparing chitosan oligosaccharide by acid hydrolysis, chitosan can generate long-chain partial hydrolysis under the acidic condition to form a plurality of fragments with different relative molecular masses, but the final product has high monosaccharide content and low yield of oligosaccharide above trimerization; the biological enzymolysis method is usually a chitosanase hydrolysis method, the chitosanase specifically hydrolyzes beta-1, 4 glycosidic bond in chitosan molecule in an incision action mode, the reaction condition is mild and easy to control, the molecular weight distribution of the product is uniform, the monosaccharide content is very low, and no other by-product exists, thus ensuring the safety of the product.

However, the number average molecule of the chitosan oligosaccharide products on the market is 2 KDa-3 KDa, the chitosan oligosaccharide products are not suitable for preparing weight-reducing chitosan oligosaccharide, the research on the chitosan oligosaccharide (the deacetylation degree is more than or equal to 90 percent, and the number average molecular weight is less than or equal to 1KDa) is developed, and the method has great significance for developing the first new natural saccharide weight-reducing raw material.

Disclosure of Invention

In view of the above, the technical problem to be solved by the present invention is to provide a method for preparing chitosan oligosaccharide with number average molecular weight less than or equal to 1Kda and a weight-reducing tablet.

The invention provides a preparation method of chitosan oligosaccharide, which comprises the following steps:

s1) swelling chitosan in an acidic aqueous solution, adding chitosan enzyme, and heating for enzymolysis to obtain an enzymolysis solution;

Preferably, the deacetylation degree of the chitosan is more than or equal to 90 percent; the mass concentration of chitosan in the acidic aqueous solution is preferably 2-8%; the pH value of the acidic aqueous solution is 4.5-5.5.

Preferably, the swelling temperature is 50-60 ℃; the swelling time is 3-5 h; the temperature of the enzymolysis is 50-60 ℃; the enzymolysis time is 3-5 h; the temperature of the enzyme deactivation treatment is 75-85 ℃; the enzyme deactivation treatment time is 10-20 min.

Preferably, the ratio of the chitosanase to the chitosan is (30-40) U: 1g of the total weight of the composition.

Preferably, the clarification treatment is to add a clarifying agent and then filter; the mass of the clarifying agent is 0.3-0.8% of that of the acidic aqueous solution; the clarifying agent is diatomite.

Preferably, the method further comprises the following steps:

Preferably, the temperature of the reduced pressure concentration is 75-85 ℃; and carrying out reduced pressure concentration until the dry matter content is 15-30%.

Preferably, the air inlet temperature of the spray drying is 180-220 ℃, and the air outlet temperature is 75-85 ℃.

The invention also provides a weight-losing tablet which comprises the chitosan oligosaccharide prepared by the preparation method.

Preferably, the method comprises the following steps:

the invention provides a preparation method of chitosan oligosaccharide, which comprises the following steps: s1) swelling chitosan in an acidic aqueous solution, adding chitosan enzyme, and heating for enzymolysis to obtain an enzymolysis solution; s2) carrying out enzyme deactivation treatment and clarification treatment on the enzymolysis liquid to obtain the liquid chitosan oligosaccharide with the number average molecular weight less than or equal to 1 Kda. Compared with the prior art, the invention adopts the biological enzymolysis method to prepare the chitosan oligosaccharide with the number average molecular weight less than or equal to 1Kda, carries out enzyme deactivation treatment after the enzymolysis is finished, does not introduce other substances, ensures the safety of the product, and provides reliable raw materials for preparing natural substance weight-reducing products.

The weight-reducing tablet is further prepared from natural saccharide chitosan oligosaccharide (the deacetylation degree is more than or equal to 90 percent, and the number average molecular weight is less than or equal to 1KDa), has the effects of increasing the excretion of lipid, improving the blood lipid disorder, reducing the hyperplasia of adipose tissues and reducing weight and fat under the condition of not affecting appetite and having no diarrhea, and is the first new natural saccharide weight-reducing raw material.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides a preparation method of chitosan oligosaccharide, which comprises the following steps: s1) swelling chitosan in an acidic aqueous solution, adding chitosan enzyme, and heating for enzymolysis to obtain an enzymolysis solution; s2) carrying out enzyme deactivation treatment and clarification treatment on the enzymolysis liquid to obtain the liquid chitosan oligosaccharide with the number average molecular weight less than or equal to 1 Kda.

The chitosan oligosaccharide with the number average molecular weight not more than 1KDa is an oligomer formed by connecting 3-6 glucosamine and N-acetylglucosamine by beta-1, 4 glycosidic bonds, has good water solubility, nearly 100 percent of human intestinal absorption rate, no toxic or side effect on a human body, and unique physiological activity and function.

The present invention is not particularly limited in terms of the source of all raw materials, and may be commercially available. Wherein, the deacetylation degree of the chitosan is preferably more than or equal to 90%.

Swelling chitosan in an acidic aqueous solution; the pH value of the acidic aqueous solution is preferably 4.5-5.5; the pH value of the acidic aqueous solution is preferably adjusted by lactic acid; the lactic acid is preferably food grade lactic acid; the mass concentration of chitosan in the acidic aqueous solution is preferably 2-8%; in some embodiments provided herein, the mass concentration of chitosan in the acidic aqueous solution is preferably 2%; in some embodiments provided herein, the mass concentration of chitosan in the acidic aqueous solution is preferably 6%; in other embodiments provided herein, the chitosan in acidic aqueous solution has a mass concentration of preferably 8%; the swelling temperature is preferably 50-60 ℃; the swelling time is preferably 3-5 h. In the invention, preferably, the chitosan is mixed with water to obtain a chitosan solution, and then acid is added to adjust the pH value of the solution for swelling; the water is preferably purified water.

After swelling, adding chitosanase, and heating for enzymolysis to obtain an enzymolysis liquid; the preferred ratio of the chitosanase to the chitosan is (30-40) U: 1g of a compound; the temperature of the enzymolysis is preferably 50-60 ℃; the enzymolysis time is preferably 3-5 h.

Carrying out enzyme deactivation treatment on the enzymolysis liquid; the temperature of the enzyme deactivation treatment is preferably 75-85 ℃; the time of enzyme deactivation treatment is preferably 10-20.

After enzyme deactivation treatment, preferably cooling, and then performing clarification treatment to obtain liquid chitosan oligosaccharide with the number average molecular weight of less than or equal to 1 Kda; the clarification treatment is preferably carried out after a clarifying agent is added; the mass of the clarifying agent is preferably 0.3-0.8% of that of the acidic aqueous solution (namely chitosan and water); the clarifying agent is preferably diatomaceous earth.

According to the present invention, it is preferable that the liquid chitosan oligosaccharide having a number average molecular weight of 1Kda or less is concentrated under reduced pressure and then spray-dried to obtain a solid chitosan oligosaccharide having a number average molecular weight of 1Kda or less; the temperature of the reduced pressure concentration is preferably 75-85 ℃; the reduced pressure concentration is preferably carried out until the dry matter content is 15 to 30 percent; the air inlet temperature of the spray drying is preferably 180-220 ℃, and the air outlet temperature is preferably 75-85 ℃.

The invention adopts a biological enzymolysis method to prepare the chitosan oligosaccharide with the number average molecular weight less than or equal to 1Kda, carries out enzyme deactivation treatment after the enzymolysis is finished, does not introduce other substances, ensures the safety of the product, and provides a reliable raw material for preparing natural substance weight-reducing products.

The invention also provides a weight-reducing tablet, which comprises the chitosan oligosaccharide with the number-average molecular weight of less than or equal to 1KDa prepared by the method, and more preferably the chitosan oligosaccharide with the deacetylation degree of more than or equal to 90% and the number-average molecular weight of less than or equal to 1 KDa.

The chitosan oligosaccharide (the deacetylation degree is more than or equal to 90 percent and the number average molecular weight is less than or equal to 1KDa) reduces the formation of fat of 3T3-L1 preadipocytes, inhibits the differentiation and maturation of the preadipocyte, regulates the level of apolipoprotein B in stomach, inhibits the accumulation of fat in vivo, reduces the accumulation of fat cells, thereby reducing the contents of Total Cholesterol (TC), Triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in serum and improving the blood fat level; meanwhile, the chitosan oligosaccharide (the deacetylation degree is more than or equal to 90 percent, and the number average molecular weight is less than or equal to 1KDa) inhibits the activity of pancreatic lipase, and is combined with bile acid to reduce the absorption of fat by intestinal tracts and increase the fat excretion in excrement, thereby achieving the effect of losing weight.

According to the present invention, preferably, the slimming tablet includes: 75-85 parts by weight of chitosan oligosaccharide with the number average molecular weight less than or equal to 1KDa prepared by the method; 5-10 parts by weight of isomalt; 0.2-0.5 parts by weight of magnesium stearate; 0.5 to 1.5 parts by weight of silica.

In some embodiments provided herein, the slimming tablet preferably comprises 80 parts by weight of chitosan oligosaccharide with number average molecular weight of less than or equal to 1 KDa; 5 parts by weight of isomalt; 0.5 part by weight of magnesium stearate; 1 part by weight of silica.

In some embodiments provided herein, the slimming tablet preferably comprises 85 parts by weight of chitosan oligosaccharide with number average molecular weight of less than or equal to 1 KDa; 6 parts by weight of isomalt; 0.2 part by weight of magnesium stearate; 1.5 parts by weight of silica.

In other embodiments provided herein, the slimming tablet preferably comprises 75.7 parts by weight of chitosan oligosaccharide with number average molecular weight of less than or equal to 1 KDa; 10 parts by weight of isomalt; 0.3 part by weight of magnesium stearate; 0.5 part by weight of silica.

The invention adopts natural saccharide chitosan oligosaccharide (the deacetylation degree is more than or equal to 90 percent and the number average molecular weight is less than or equal to 1KDa) to prepare the weight-reducing tablet, has the effects of increasing the excretion of lipid, improving the blood fat disorder and reducing the hyperplasia of adipose tissues under the condition of not influencing appetite and having no diarrhea, achieves the effects of reducing weight and fat, and is the first new natural saccharide weight-reducing raw material.

In order to further illustrate the present invention, the preparation method of chitosan oligosaccharide and the weight-reducing tablet provided by the present invention are described in detail below with reference to the examples.

The reagents used in the following examples are all commercially available; in the examples, the number average molecular weight of chitosan oligosaccharide was measured by the acetylacetone method.

Example 1

A preparation method of chitosan oligosaccharide and weight-reducing tablet with the number-average molecular weight less than or equal to 1KDa comprises the following steps:

(1) swelling chitosan:

adding 5Kg of chitosan with deacetylation degree of more than or equal to 90% into a 150L reaction kettle, adding 95L of purified water to prepare 6% (w/w) chitosan solution, adjusting pH of the solution to 4.5 with lactic acid, stirring continuously, and swelling in 50 deg.C water bath for 5 h.

(2) Preparing liquid chitosan oligosaccharide with the number average molecular weight not more than 1 KDa:

adding chitosanase 1.65X 10⁵U, stirring and reacting at 50 ℃ for 3h to obtain an enzymolysis solution, preserving the temperature in 78 ℃ water bath for 10min to inactivate enzyme, cooling the solution, adding 0.4Kg of diatomite, stirring uniformly, and filtering to obtain clear liquid chitosan oligosaccharide with the number average molecular weight of 930 Da.

(3) Preparing the solid chitosan oligosaccharide with the number average molecular weight not more than 1 KDa:

carrying out vacuum concentration on 930Da liquid chitosan oligosaccharide at 82 ℃ until the dry matter content is 30.0%, carrying out spray drying at the air inlet temperature of 180-220 ℃ and the air outlet temperature of 75-85 ℃ to obtain 4.85Kg of solid chitosan oligosaccharide with the number average molecular weight of 930 Da.

(4) Preparing a weight-reducing tablet:

4Kg of powdery solid chitosan oligosaccharide, 250g of isomalt, 25g of magnesium stearate and 50g of silicon dioxide are uniformly mixed to prepare the chitosan oligosaccharide weight-reducing tablet.

Example 2

A preparation method of chitosan oligosaccharide and weight-reducing tablet with the number-average molecular weight less than or equal to 1KDa comprises the following steps:

(1) swelling chitosan:

adding 50Kg of chitosan with deacetylation degree of more than or equal to 90% into a 5T reaction kettle, adding 2450L of purified water to prepare 2% (w/w) chitosan solution, adjusting pH of the solution to 5.5 with lactic acid, stirring continuously, and swelling in 57 deg.C water bath for 3 h.

(2) Preparing liquid chitosan oligosaccharide with the number average molecular weight not more than 1 KDa:

adding chitosanase 1.5X 10⁶U, stirring and reacting at 57 ℃ for 5h to obtain an enzymolysis solution, keeping the temperature of the obtained enzymolysis solution in a water bath at 85 ℃ for 12min for enzyme inactivation, cooling the solution, adding 20Kg of diatomite, stirring uniformly, and filtering to obtain clear liquid chitosan oligosaccharide with the number average molecular weight of 890 Da.

(3) Preparing the solid chitosan oligosaccharide with the number average molecular weight not more than 1 KDa:

the 890Da liquid chitosan oligosaccharide is decompressed and concentrated at 75 ℃ until the dry matter content is 15.0 percent, and spray drying is carried out at the air inlet temperature of 180-220 ℃ and the air outlet temperature of 75-85 ℃ to obtain 47.32Kg solid chitosan oligosaccharide with the number average molecular weight of 890 Da.

(4) Preparing a weight-reducing tablet:

5Kg of powdery solid chitosan oligosaccharide, 353g of isomalt, 12g of magnesium stearate and 88g of silicon dioxide are uniformly mixed to prepare the chitosan oligosaccharide weight-reducing tablet.

Example 3

A preparation method of chitosan oligosaccharide and weight-reducing tablet with the number-average molecular weight less than or equal to 1KDa comprises the following steps:

(1) swelling chitosan:

adding 100Kg of chitosan with deacetylation degree of more than or equal to 90% into a 5T reaction kettle, adding 1150L of purified water to prepare 8% (w/w) chitosan solution, adjusting pH of the solution to 5.0 with lactic acid, stirring continuously, and swelling in 60 deg.C water bath for 4 h.

(2) Preparing liquid chitosan oligosaccharide with the number average molecular weight not more than 1 KDa:

adding chitosanase 1.0X 10⁷U, stirring and reacting at 60 ℃ for 4.5h to obtain an enzymolysis solution, keeping the temperature of the obtained enzymolysis solution in a water bath at 75 ℃ for 20min to inactivate enzyme, cooling the solution, adding 3.75Kg of diatomite, stirring uniformly, and filtering to obtain clear liquid chitosan oligosaccharide with the number average molecular weight of 913 Da.

(3) Preparing the solid chitosan oligosaccharide with the number average molecular weight not more than 1 KDa:

the 913Da liquid chitosan oligosaccharide is decompressed and concentrated at 85 ℃ until the dry matter content is 23.6 percent, and the solid chitosan oligosaccharide with the number average molecular weight of 96.30Kg is obtained by spray drying at the air inlet temperature of 180 to 220 ℃ and the air outlet temperature of 75 to 85 ℃.

(4) Preparing a weight-reducing tablet:

5Kg of powdery solid chitosan oligosaccharide, 667g of isomalt, 20g of magnesium stearate and 33g of silicon dioxide are mixed uniformly to prepare the chitosan oligosaccharide weight-reducing tablet.

Comparative example

The preparation method of the chitosan oligosaccharide with the number average molecular weight of less than or equal to 1Kda is compared and analyzed with the method for preparing the chitosan oligosaccharide by decomposing the chitosan oligosaccharide with patents CN101845471B, CN107988287A, CN107739418A, CN108913735B, CN109136307A and CN110699406A, and the analysis result is shown in Table 1.

TABLE 1 comparison of the preparation method of chitooligosaccharide with number average molecular weight not more than 1Kda of the present invention with the preparation method of chitooligosaccharide by ordinary enzymolysis

As can be seen from Table 1, the preparation method of chitosan oligosaccharide with number average molecular weight of less than or equal to 1Kda of the invention is compared with the preparation method of chitosan oligosaccharide by hydrolysis of patents CN101845471B, CN107988287A, CN107739418A, CN108913735B, CN109136307A and CN110699406A, the pH value selected by chitosan swelling provides the optimal environment for the sufficient swelling of chitosan and the enzymolysis of chitosan, the optimal enzyme amount, temperature and time are selected under the condition that the selected enzymolysis chitosan needs enzyme types, the chitosan oligosaccharide with number average molecular weight of less than or equal to 1Kda is obtained by the invention, and the chitosan oligosaccharide with full molecular weight is obtained by patents CN101845471B, CN107988287A, CN107739418A, CN108913735B, CN109136307A and CN 110699406A.

Test examples

Experiment on influence of chitosan oligosaccharide weight-reducing tablet prepared in embodiment of the invention on weight-reducing function of rats

And (3) testing a sample: the chitosan oligosaccharide weight-reducing tablets prepared in the embodiments 1, 2 and 3 of the invention.

Experimental animals: 70 male SPF SD rats with the weight of 200-220 g.

The experimental method comprises the following steps:

(1) establishing a high-fat feed induced obesity rat model: 70 male SD rats are fed in the central SPF environment of the experimental animal at the temperature of 22-26 ℃ and the relative humidity of 55-65%. After the animals are fed with the common feed adaptively for 15 days, the animals are randomly divided into two groups, wherein one group is composed of 10 SD rats and is fed with the common feed and normal drinking water as a blank group; in the other group, 60 SD rats are fed with high-fat feed and drinking deionized water, 10 rats with slow weight gain are removed after 15 days of feeding, the remaining 50 SD rats are continuously fed with the high-fat feed for 30 days, and the SD rats fed with the high-fat feed have the weight which is 20% higher than that of the SD rats fed with the common feed, namely the molding success is achieved.

(2) Weight-reducing experiment: 50 SD obese rats are randomly divided into a model group, a positive control group, an example 1 group, an example 2 group and an example 3 group, each group comprises 10 animals, each group is continuously fed with high-fat feed and deionized water (10 mL/KgBw is given for gastric lavage), 125mg/KgBw (calculated by chitosan oligosaccharide and equivalent to 5 times of daily recommended amount of a human body) of the crushed chitosan oligosaccharide weight-reducing tablets prepared in the examples 1, 2 and 3 is added into the deionized water of the examples 1, 2 and 3, 60d is continuously fed into the deionized water of the positive control group, the daily weight and food intake of the rats are recorded, after the experiment is finished, the rats are fasted for 12-16 hours, 10% chloral hydrate is used for anesthesia, fat around the testis, fat around the kidney, subcutaneous fat is taken and the TC, TG, C, LDL-C, HDL-C and TC in excrement are detected, TG, TBA content.

The experimental results are as follows:

(1) effect of test samples on body weight of obese rats: as can be seen from Table 2 below, the body weight of the white control group rats was significantly higher at the beginning of the experiment than that of the rest of the experimental groups, indicating that the modeling was successful. At the end of the test, the weight gains of rats in the blank control group, the positive control group and the groups of examples 1, 2 and 3 are all significantly lower than those of the model group, which indicates that the positive control group and the groups of examples 1, 2 and 3 can significantly slow down the weight gain of SD obese rats to achieve the effect of losing weight, but a small amount of rats in the positive control group have diarrhea in the experimental process, and the rest experimental groups have no diarrhea.

TABLE 2 weight changes of rats in each group before and after the test