阅读说明：本技术 用于液相色谱检测的样品净化装置 (Sample purification device for liquid chromatography detection ) 是由 薛同闯 于 2021-07-06 设计创作，主要内容包括：本发明公开了一种用于液相色谱检测的样品净化装置,包括位于振动摇床上的缓冲箱,缓冲箱内分别设有与进液管连接的一级净化组件、与出液管连接的二级净化组件,一级净化组件过滤的液体能经缓冲箱进入到二级净化组件内。一级净化组件包括内外套设的采样管、一级净化管,采样管的上端通过密封柱塞与进液管连接,下端通过过滤漏板与一级净化管连通；一级净化管、进液管之间还设有环形滤膜。二级净化组件包括倒置的二级净化管,二级净化管的下端设有与缓冲箱连通的开口,上端密封插设有出液管。开口与缓冲箱的内底壁之间留有间隙。本发明的样品净化装置通过多级过滤加强了过滤效果,能有效延缓色谱柱的堵塞现象。(The invention discloses a sample purification device for liquid chromatography detection, which comprises a buffer tank positioned on a vibration table, wherein a primary purification component connected with a liquid inlet pipe and a secondary purification component connected with a liquid outlet pipe are respectively arranged in the buffer tank, and liquid filtered by the primary purification component can enter the secondary purification component through the buffer tank. The primary purification assembly comprises a sampling pipe and a primary purification pipe which are sleeved inside and outside, the upper end of the sampling pipe is connected with the liquid inlet pipe through a sealing plunger, and the lower end of the sampling pipe is communicated with the primary purification pipe through a filtering leakage plate; an annular filter membrane is also arranged between the first-stage purifying pipe and the liquid inlet pipe. The second grade purifies the subassembly and includes the second grade purge tube of invering, and the lower extreme of second grade purge tube is equipped with the opening with the baffle-box intercommunication, and sealed the inserting of upper end is equipped with the drain pipe. A gap is reserved between the opening and the inner bottom wall of the buffer box. The sample purification device provided by the invention enhances the filtering effect through multi-stage filtering, and can effectively delay the blockage phenomenon of the chromatographic column.)

1. A sample purifier for liquid chromatography detection which characterized in that: the device comprises a buffer tank (2) positioned on a vibrating table (1), wherein a primary purification component connected with a liquid inlet pipe (3) and a secondary purification component connected with a liquid outlet pipe (4) are respectively arranged in the buffer tank (2), and liquid filtered by the primary purification component can enter the secondary purification component through the buffer tank (2); the primary purification assembly comprises a sampling pipe (51) and a primary purification pipe (52), wherein the sampling pipe (51) and the primary purification pipe are sleeved inside and outside, the upper end of the sampling pipe (51) is connected with the liquid inlet pipe (3) through a sealing plunger (53), and the lower end of the sampling pipe is communicated with the primary purification pipe (52) through a filtering leakage plate (54); an annular filter membrane (55) is also arranged between the primary purification pipe (52) and the liquid inlet pipe (3); the secondary purification component comprises an inverted secondary purification pipe (61), the lower end of the secondary purification pipe (61) is provided with an opening communicated with the buffer tank (2), and the upper end of the secondary purification pipe is hermetically inserted with the liquid outlet pipe (4); a gap is reserved between the opening and the inner bottom wall of the buffer box (2).

2. The sample purification apparatus of claim 1, wherein: at least two layers of secondary filter membranes (62) which are arranged in a stacked mode are arranged in the secondary purification tube (61), and a gap is reserved between every two adjacent layers of secondary filter membranes (62).

3. The sample purification apparatus of claim 2, wherein: the inner wall of the secondary purification pipe (61) is provided with a plurality of annular grooves (611), and the secondary filter membrane (62) can be assembled in any annular groove (611) through a gasket (63) in an interference manner.

4. The sample purification apparatus of claim 3, wherein: the annular groove (611) at the uppermost layer is positioned below the liquid outlet pipe (4).

5. The sample purification apparatus of claim 4, wherein: the filter pore diameters of the multiple layers of secondary filter membranes (62) are sequentially reduced from bottom to top.

6. The sample purification apparatus according to any one of claims 1 to 5, wherein: the upper end of the primary purification pipe (52) is also provided with an exhaust hole (523), and a one-way valve plate (524) capable of opening and closing the exhaust hole (523) is arranged at the exhaust hole (523).

7. The sample purification apparatus of claim 6, wherein: a first mounting clamping seat is fixedly connected to the inner wall of the lower end part of the primary purification pipe (52), and a second mounting clamping seat matched with the first mounting clamping seat is arranged on the outer wall of the lower end part of the sampling pipe (51); the annular filter membrane (55) is arranged between the first installation clamping seat and the second installation clamping seat in a sealing mode.

8. The sample purification apparatus of claim 7, wherein: a plurality of liquid outlet holes (522) communicated with the buffer box (2) are formed in the side wall of the upper end part of the primary purification pipe (52), and the liquid outlet holes (522) are located above the annular filter membrane (55).

Technical Field

The invention relates to the field of liquid chromatography detection, in particular to a sample purification device for liquid chromatography detection.

Background

Liquid chromatography is a separation and analysis technique characterized by the use of liquid as the mobile phase and the stationary phase in various forms, such as paper, sheet and packed bed. The separation mechanism can be classified into adsorption chromatography, partition chromatography, ion exchange chromatography, and gel chromatography. In liquid chromatography detection, in order to improve the analysis accuracy and separation effect, the sample is generally pretreated to remove impurities in the matrix, which interfere with the analysis of the sample. The existing sample pretreatment device mostly directly adopts a filter membrane to filter, the filter effect is limited, and partial impurities still enter a detector to cause the blockage of a chromatographic column.

Disclosure of Invention

In order to overcome the above disadvantages, the present invention provides a sample purification device for liquid chromatography detection, which enhances the filtration effect by multi-stage filtration and can effectively delay the blocking phenomenon of a chromatographic column.

In order to achieve the above purposes, the invention adopts the technical scheme that: the utility model provides a sample purification device for liquid chromatogram detects, is including being located the baffle-box on the vibration shaking table, be equipped with the one-level purification subassembly of being connected with the feed liquor pipe in the baffle-box respectively, purify the subassembly with the second grade of drain pipe connection, the filterable liquid of one-level purification subassembly can enter into the second grade through the baffle-box and purify the subassembly in. The primary purification assembly comprises a sampling pipe and a primary purification pipe which are sleeved inside and outside, the upper end of the sampling pipe is connected with the liquid inlet pipe through a sealing plunger, and the lower end of the sampling pipe is communicated with the primary purification pipe through a filtering leakage plate; an annular filter membrane is also arranged between the primary purification pipe and the liquid inlet pipe. The second grade purifies the subassembly including the second grade purge tube of invering, the lower extreme of second grade purge tube be equipped with the opening of baffle-box intercommunication, the upper end is sealed to be inserted and is equipped with the drain pipe. A gap is reserved between the opening and the inner bottom wall of the buffer box.

In the sample liquid enters into the sampling tube through the feed liquor pipe, carries out the coarse filtration via filtering the bushing, and large granule thing is held back in the sampling tube, and all the other sample liquids enter into one-level purifying tube, and when the sample liquid that enters into one-level purifying tube highly reached the height of annular filter membrane, the sample liquid filters through annular filter membrane once more, and the sample liquid after the filtration enters into the baffle-box to purify in entering into the second grade purifying tube through the opening, then via the drain pipe discharge.

The invention has the beneficial effects that: the multi-stage filtration of the sample liquid is realized through the arrangement of the first-stage purification pipe and the second-stage purification pipe, and the filtration effect of the sample liquid is effectively enhanced; meanwhile, the sample liquid is filtered more fully through the backflow of the sample liquid in the primary purification pipe and the secondary purification pipe; at purifier during operation, the vibration shaking table drives the baffle-box vibration to accelerate appearance liquid flow rate, improve filtration efficiency, simultaneously can alleviate the jam phenomenon in filtration bushing, annular filter membrane and the second grade purge tube through the vibration.

Further, at least two layers of secondary filter membranes which are arranged in a stacked mode are arranged in the secondary purification pipe, and a gap is reserved between every two adjacent layers of secondary filter membranes. And the multi-stage filtration is carried out on the sample liquid through the arrangement of the multi-layer secondary filter membranes, so that the filtering effect is enhanced.

Further, a plurality of annular grooves are formed in the inner wall of the secondary purification pipe, and the secondary filter membrane can be assembled in any annular groove in an interference mode through a gasket.

Further, the annular groove on the uppermost layer is located below the liquid outlet pipe.

Further, the aperture of the filter pores of the secondary filter membranes is reduced from bottom to top in sequence.

Further, the upper end of the primary purification pipe is also provided with an exhaust hole, and the exhaust hole is provided with a one-way valve plate capable of opening and closing the exhaust hole. Gas in the one-level purifying pipe can be timely discharged through the arrangement of the exhaust holes, and the situation that the sample liquid flows and is blocked due to overhigh air pressure in the one-level purifying pipe in the purifying process is prevented.

Further, an installation clamping seat is fixedly connected to the inner wall of the lower end portion of the primary purification pipe, and a second installation clamping seat matched with the first installation clamping seat is arranged on the outer wall of the lower end portion of the sampling pipe. The annular filter membrane is arranged between the first installation clamping seat and the second installation clamping seat in a sealing mode.

Furthermore, a plurality of liquid outlet holes communicated with the buffer tank are formed in the side wall of the upper end part of the primary purification pipe, and the liquid outlet holes are located above the annular filter membrane.

Drawings

FIG. 1 is a schematic structural diagram of an embodiment of the present invention;

FIG. 2 is a partial enlarged view of portion A of FIG. 1;

FIG. 3 is a partial enlarged view of the portion B in FIG. 1;

fig. 4 is a partially enlarged view of a portion C in fig. 1.

In the figure:

1-vibrating a shaking table; 2-a buffer tank; 3-a liquid inlet pipe; 4-a liquid outlet pipe; 51-a sampling tube; 511-mount pad II; 52-primary purification pipe; 521-a first mounting seat; 522-liquid outlet hole; 523-vent hole; 524-one-way valve plate; 53-sealing the plunger; 54-a filter tip plate; 55-ring filter membrane; 61-a secondary purification pipe; 611-an annular groove; 62-secondary filter membrane; 63-pad.

Detailed Description

The following detailed description of the preferred embodiments of the present invention, taken in conjunction with the accompanying drawings, will make the advantages and features of the invention easier to understand by those skilled in the art, and thus will clearly and clearly define the scope of the invention.

Examples

Referring to the attached drawings 1-4, the sample purification device for liquid chromatography detection of the invention comprises a buffer tank 2 positioned on a vibration table 1, a primary purification component connected with a liquid inlet pipe 3 and a secondary purification component connected with a liquid outlet pipe 4 are respectively arranged in the buffer tank 2, and liquid filtered by the primary purification component can enter the secondary purification component through the buffer tank 2.

The one-level purification assembly comprises an inner sleeve and an outer sleeve, wherein the inner sleeve and the outer sleeve are respectively provided with a sampling pipe 51 and a one-level purification pipe 52, the upper end of the sampling pipe 51 is connected with the liquid inlet pipe 3 through a sealing plunger 53, and the lower end of the sampling pipe is communicated with the one-level purification pipe 52 through a filtering leakage plate 54. An annular filter membrane 55 is also arranged between the primary purification pipe 52 and the liquid inlet pipe 3.

Specifically, the inner wall of the lower end part of the primary purification pipe 52 is fixedly connected with a first installation clamping seat 521, and the outer wall of the lower end part of the sampling pipe 51 is provided with a second installation clamping seat 511 matched with the first installation clamping seat 521. The annular filter membrane 55 is arranged between the first installation clamping seat 521 and the second installation clamping seat 511 in a sealing mode.

A plurality of liquid outlet holes 522 communicated with the buffer tank 2 are formed in the side wall of the upper end part of the primary purification pipe 52, and the liquid outlet holes 522 are positioned above the annular filter membrane 55.

When the sample liquid enters the sampling pipe 51 through the liquid inlet pipe 3, firstly, coarse filtration is carried out through the filtering leakage plate 54 to remove large-particle impurities in the sample liquid; the coarsely filtered sample liquid enters the bottom of the primary purification pipe 52, and when the sample liquid at the bottom of the primary purification pipe 52 is accumulated to contact the annular filter membrane 55, the sample liquid is further filtered by the annular filter membrane 55 to remove smaller particle impurities in the sample liquid; the sample liquid filtered by the annular filter membrane 55 enters the primary purification pipe 52 above the annular filter membrane 55 and enters the buffer tank through the liquid outlet 522.

In this embodiment, in the primary purification pipe 52, since the sample liquid passes through the annular filter membrane 55 from bottom to top (i.e. the sample liquid flows backwards), the upper end of the primary purification pipe 52 is further provided with an exhaust hole 523, and a check valve plate 524 capable of opening and closing the exhaust hole 523 is arranged at the exhaust hole 523. The gas in the first-stage purification pipe 52 can be discharged in time through the arrangement of the exhaust holes 523, and the situation that the sample liquid flows and is blocked due to overhigh air pressure in the first-stage purification pipe 52 in the purification process is prevented.

The second grade purifies subassembly including the second grade purge tube 61 of invering, the lower extreme of second grade purge tube 61 be equipped with the opening of baffle-box 2 intercommunication, the upper end is sealed to be inserted and is equipped with drain pipe 4. A gap is left between the opening and the inner bottom wall of the buffer tank 2. At least two layers of secondary filter membranes 62 which are arranged in a stacked mode are arranged in the secondary purification tube 61, and a gap is reserved between every two adjacent layers of secondary filter membranes 62. The filter pore diameters of the multiple layers of secondary filter membranes 62 are sequentially reduced from bottom to top. The multi-stage filtration is carried out on the sample liquid through the arrangement of the multi-layer secondary filter membrane 62, so that the filtering effect is enhanced.

Specifically, a plurality of annular grooves 611 are formed in the inner wall of the secondary purification pipe 61, and the secondary filter membrane 62 can be interference-assembled in any of the annular grooves 611 through a gasket 63. The uppermost annular groove 611 is located below the liquid outlet pipe 4.

When the sample liquid in the buffer tank 2 reaches the opening height of the secondary purification tube 61, the sample liquid can sequentially pass through the multilayer secondary filter membrane 62 from bottom to top for multistage filtration under the action of the water suction pump of the liquid outlet tube 61, and is finally discharged through the liquid outlet tube.

The primary purification pipe 52 and the secondary purification pipe 61 are arranged to realize the multi-stage filtration of the sample liquid, so that the filtration effect of the sample liquid is effectively enhanced; meanwhile, the sample liquid is filtered more fully through the backflow of the sample liquid in the primary purification pipe 52 and the secondary purification pipe 61; when the purification device works, the vibration table 1 drives the buffer tank 2 to vibrate so as to accelerate the flow rate of the sample liquid and improve the filtration efficiency, and simultaneously, the blockage phenomenon in the filtration leakage plate 54, the annular filtration membrane 55 and the secondary filtration membrane 62 can be reduced through vibration.

The above embodiments are merely illustrative of the technical concept and features of the present invention, and the present invention is not limited thereto, and any equivalent changes or modifications made according to the spirit of the present invention should be included in the scope of the present invention.