阅读说明：本技术 一种利用玉米淀粉发酵生产丁酸的方法 (Method for producing butyric acid by fermenting corn starch ) 是由 李冰 吕向云 曹华伟 娄百勇 李学成 杨永 于 2021-01-15 设计创作，主要内容包括：本发明公开了一种利用玉米淀粉发酵生产丁酸的方法,包括以下步骤：发酵生产方法包括种子培养和发酵培养,丁酸发酵菌体接种于种子罐培养基进行种子培养,然后接种于发酵罐培养基中进行发酵培养,所述发酵罐培养基组成包括：玉米淀粉25～60g/L、淀粉酶0.004～0.01g/L、有机氮源2～8g/L、(NH-4)-2SO-4 2g/L、K-2HPO-4 1g/L、KH-2PO-4 1g/L、MgSO-4·7H-2O 0.1g/L、FeSO-4·7H-2O 0.015g/L、CaCl-2·2H-2O 0.015g/L、MnSO-4·H-2O 0.01g/L、CoCl-2·6H-2O 0.02g/L、ZnSO-4·7H-2O 0.002g/L,余量为水。本发明提供的利用玉米淀粉发酵生产丁酸的方法,在此培养基基础上将发酵培养基中的速效碳源葡萄糖用迟效碳源玉米淀粉(或玉米面粉)部分替代及全部替代,进而降低原料使用成本,减少发酵过中葡糖糖单独消罐带来的设备成本、人力成本、用电成本等问题,提高发酵产能。(The invention discloses a method for producing butyric acid by fermenting corn starch, which comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein butyric acid fermentation thalli is inoculated in a seed tank culture medium for seed culture and then is inoculated in a fermentation tank culture medium for fermentation culture, and the fermentation tank culture medium comprises the following components: 25-60 g/L of corn starch, 0.004-0.01 g/L of amylase, 2-8 g/L of organic nitrogen source, (NH) 4 ) 2 SO 4 2g/L、K 2 HPO 4 1g/L、KH 2 PO 4 1g/L、MgSO 4 ·7H 2 O 0.1g/L、FeSO 4 ·7H 2 O 0.015g/L、CaCl 2 ·2H 2 O 0.015g/L、MnSO 4 ·H 2 O 0.01g/L、CoCl 2 ·6H 2 O 0.02g/L、ZnSO 4 ·7H 2 O0.002 g/L, and the balance being water. The invention provides a method for producing butyric acid by fermenting corn starch, which is characterized in that on the basis of the culture medium, a quick-acting carbon source glucose in the fermentation culture medium uses a slow-acting carbon source corn starch (or corn flour)The method can be used for partial replacement or complete replacement, so that the use cost of raw materials is reduced, the problems of equipment cost, labor cost, electricity consumption cost and the like caused by independent tank elimination of glucose in fermentation are solved, and the fermentation capacity is improved.)

1. A method for producing butyric acid by fermenting corn starch is characterized by comprising the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein the butyric acid fermentation thalli is inoculated in a seed tank culture medium for seed culture,then inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the fermentation tank culture medium comprises the following components: 25-60 g/L of corn starch, 0.004-0.01 g/L of amylase, 2-8 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water.

2. The method for producing butyric acid by fermentation of corn starch according to claim 1, wherein the culture medium composition of the fermentation tank further comprises 10-30 g/L of glucose.

3. The method for producing butyric acid by fermentation of corn starch according to claim 1, wherein the composition of the seed tank medium comprises: glucose 20g/L, yeast extract 2g/L, peptone 4g/L, (NH)₄)₂SO₄ 2g/L、K₂HPO₄1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water.

Technical Field

The invention belongs to the technical field of butyric acid fermentation, and particularly relates to a method for producing butyric acid by fermenting corn starch.

Background

Butyric acid is a platform compound, is widely applied to industries such as food, chemical industry, pharmacy and the like, and has global market demand of about 80000t every year. There are two main ways of butyric acid production at present: chemical synthesis and microbial fermentation. The chemical synthesis method has high yield and good product specificity, but the production operation is carried out under the conditions of high temperature and high pressure, and non-renewable fossil raw materials are used. The microbial fermentation method can also produce butyric acid, because the biomass raw material used is carbon neutral, and has great advantages in relieving global warming and greenhouse effect, using renewable resources and the like compared with the chemical synthesis method and the fermentation method.

The carbon source and the nitrogen source in the existing fermentation medium formula can basically meet the growth requirement of butyric acid fermentation thalli, but the cost for producing butyric acid by fermenting glucose by using the carbon source is relatively high, and the glucose needs to be consumed in a tank after being consumed separately, so the cost is increased more. The method reduces the cost of raw materials, equipment, manpower and the like by replacing the method on the basis of ensuring the yield of butyric acid fermentation, improves the utilization rate of the equipment and further improves the yield of butyric acid fermentation.

Disclosure of Invention

The invention aims to provide a method for producing butyric acid by fermenting corn starch, which saves resources, has low cost and stable product quality and is suitable for industrial production.

In order to achieve the purpose, the invention adopts the technical scheme that:

a method for producing butyric acid by fermenting corn starch comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein butyric acid fermentation thalli is inoculated in a seed tank culture medium for seed culture and then is inoculated in a fermentation tank culture medium for fermentation culture, and the fermentation tank culture medium comprises the following components: 25-60 g/L of corn starch, 0.004-0.01 g/L of amylase, 2-8 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water.

Furthermore, the culture medium composition of the fermentation tank also comprises 10-30 g/L of glucose.

Further, the composition of the seeding tank medium comprises: glucose 20g/L, yeast extract 2g/L, peptone 4g/L, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water.

The invention has the advantages that:

according to the method for producing butyric acid by fermenting corn starch, glucose serving as a quick-acting carbon source in a fermentation culture medium is partially or completely replaced by corn starch (or corn flour) serving as a slow-acting carbon source on the basis of the culture medium, so that the use cost of raw materials is reduced, the problems of equipment cost, labor cost, electricity cost and the like caused by the independent tank consumption of glucose in fermentation are solved, and the fermentation capacity is improved.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the specific examples described herein are only for explaining the present invention and are not intended to limit the present invention.

Example 1

A method for producing butyric acid by fermenting corn starch comprises the following steps: the fermentation production method comprises seed culture and fermentationCulturing, inoculating butyric acid fermentation thallus in seeding tank culture medium for seed culture, glucose 20g/L, yeast extract 2g/L, peptone 4g/L, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. Then inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the fermentation tank culture medium comprises the following components: 20-30 g/L of glucose, 25-30 g/L of corn starch, 0.004g/L of amylase, 2-8 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. And (3) respectively detecting total sugar and reducing sugar after the culture medium is sterilized, and respectively determining the content of butyric acid after the fermentation is finished.

Example 2

A method for producing butyric acid by fermenting corn starch comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein butyric acid fermentation thallus is inoculated in a seed tank culture medium for seed culture, and the culture medium comprises 20g/L glucose, 2g/L yeast extract, 4g/L peptone and (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. Then inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the fermentation tank culture medium comprises the following components: 10-12 g/L of glucose and corn starch40-58 g/L, 0.006g/L amylase, 2-8 g/L organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. And (3) respectively detecting total sugar and reducing sugar after the culture medium is sterilized, and respectively determining the content of butyric acid after the fermentation is finished.

Example 3

A method for producing butyric acid by fermenting corn starch comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein butyric acid fermentation thallus is inoculated in a seed tank culture medium for seed culture, and the culture medium comprises 20g/L glucose, 2g/L yeast extract, 4g/L peptone and (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. Then inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the fermentation tank culture medium comprises the following components: 50-60 g/L of corn starch, 0.01g/L of amylase, 2-8 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. And (3) respectively detecting total sugar and reducing sugar after the culture medium is sterilized, and respectively determining the content of butyric acid after the fermentation is finished.

Comparative example

A method for producing butyric acid by fermenting corn starch,the method comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein the butyric acid fermentation thalli is inoculated in a seed tank culture medium for seed culture, and the seed tank culture medium comprises the following components: glucose 20g/L, yeast extract 2g/L, peptone 4g/L, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. Then inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the fermentation tank culture medium comprises the following components: 50-60 g/L glucose, 2-8 g/L organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. And (3) respectively detecting total sugar and reducing sugar after the culture medium is sterilized, and respectively determining the content of butyric acid after the fermentation is finished.

Comparison of the effects of examples and comparative examples

The fermentation period of the comparative example is 48-52h, and the content of butyric acid is 47-55 g/L; the fermentation period of the embodiment 1 is 48-52h, and the content of butyric acid is 47-57 g/L; the fermentation period of the embodiment 2 is 48-51h, and the content of butyric acid is 47-56 g/L; the fermentation period of the example 3 is 48-51h, and the butyric acid content is 48-55 g/L.

From the above results, it can be seen that corn starch can completely replace glucose, and further produce butyric acid by fermentation.