阅读说明：本技术 一种利用酒糟水发酵生产丁酸的方法 (Method for producing butyric acid by fermenting distiller's grain water ) 是由 吕向云 曹华伟 杨永 李学成 娄百勇 宋玉忠 于 2021-01-15 设计创作，主要内容包括：本发明公开了一种利用酒糟水发酵生产丁酸的方法,包括以下步骤：发酵生产方法包括种子培养和发酵培养,丁酸发酵菌体接种于种子罐培养基进行种子培养,然后接种于发酵罐培养基中进行发酵培养,所述发酵罐培养基组成包括：酒槽水50～200g/L、有机氮源2～5g/L、(NH-4)-2SO-(4 )2g/L、K-2HPO-4 1g/L、KH-2PO-4 1g/L、MgSO-4·7H-2O 0.1g/L、FeSO-4·7H-2O 0.015g/L、CaCl-2·2H-2O 0.015g/L、MnSO-4·H-2O 0.01g/L、CoCl-2·6H-2O 0.02g/L、ZnSO-4·7H-2O 0.002g/L,余量为水。本发明提供的利用酒糟水发酵生产丁酸的方法,在培养基基础上将发酵培养基中的碳源葡萄糖及有机氮源部分用酒槽水替代,既能解决环保问题,变废为宝,又能降低丁酸发酵原料使用成本,具有较高的经济效益、环保效益和社会效益。(The invention discloses a method for producing butyric acid by fermenting distilled grain water, which comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein butyric acid fermentation thalli is inoculated in a seed tank culture medium for seed culture and then is inoculated in a fermentation tank culture medium for fermentation culture, and the fermentation tank culture medium comprises the following components: 50-200 g/L of wine tank water, 2-5 g/L of organic nitrogen source, (NH) 4 ) 2 SO 4 2g/L、K 2 HPO 4 1g/L、KH 2 PO 4 1g/L、MgSO 4 ·7H 2 O 0.1g/L、FeSO 4 ·7H 2 O 0.015g/L、CaCl 2 ·2H 2 O 0.015g/L、MnSO 4 ·H 2 O 0.01g/L、CoCl 2 ·6H 2 O 0.02g/L、ZnSO 4 ·7H 2 O0.002 g/L, and the balance being water. The method for producing butyric acid by fermenting the distilled grain water provided by the invention replaces part of carbon source glucose and organic nitrogen source in the fermentation culture medium with the distilled grain water on the basis of the culture medium, thereby solving the environmental protection problem, changing waste into valuable and reducing the content of the organic nitrogen sourceThe butyric acid fermentation raw material has high use cost, and has high economic benefit, environmental protection benefit and social benefit.)

1. Water fermentation by using vinasseA method for producing butyric acid by fermentation, comprising the steps of: the fermentation production method comprises seed culture and fermentation culture, wherein butyric acid fermentation thalli is inoculated in a seed tank culture medium for seed culture and then is inoculated in a fermentation tank culture medium for fermentation culture, and the fermentation tank culture medium comprises the following components: 50-200 g/L of wine tank water, 2-5 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water.

2. The method of claim 1, wherein the fermentor medium composition comprises: 130-150g/L of wine tank water, 2-5 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water.

3. The method of utilizing aqueous distillers' grains for fermentation production of butyric acid of claim 1, wherein said seed tank medium comprises: glucose 20g/L, yeast extract 2g/L, peptone 4g/L, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O 0.002g/LAnd the balance being water.

Technical Field

The invention belongs to the technical field of butyric acid fermentation, and particularly relates to a method for producing butyric acid by fermenting distiller's grains.

Background

Butyric acid is a platform compound, is widely applied to industries such as food, chemical industry, pharmacy and the like, and has global market demand of about 80000t every year. There are two main ways of butyric acid production at present: chemical synthesis and microbial fermentation. The chemical synthesis method has high yield and good product specificity, but the production operation is carried out under the conditions of high temperature and high pressure, and non-renewable fossil raw materials are used. The microbial fermentation method can also produce butyric acid, because the biomass raw material used is carbon neutral, and has great advantages in relieving global warming and greenhouse effect, using renewable resources and the like compared with the chemical synthesis method and the fermentation method. Lees and thin stillage are waste materials from alcohol production, but are rich in proteins, amino acids, organic acids, and organic matter, which provide nutrients for microbial growth.

The carbon source and the nitrogen source in the existing fermentation medium formula can basically meet the growth requirement of butyric acid fermentation thalli, but the cost for producing butyric acid by fermenting glucose by using the carbon source is relatively high, and the glucose needs to be consumed in a tank after being consumed separately, so the cost is increased more. The method reduces the cost of raw materials, equipment, manpower and the like by replacing the method on the basis of ensuring the yield of butyric acid fermentation, improves the utilization rate of the equipment and further improves the yield of butyric acid fermentation. Moreover, the waste is changed into valuable, and the method has higher economic benefit, environmental protection benefit and social benefit.

Disclosure of Invention

The invention aims to provide a method for producing butyric acid by fermenting distilled grain water, which saves resources, has low cost and stable product quality and is suitable for industrial production.

In order to achieve the purpose, the invention adopts the technical scheme that:

a method for producing butyric acid by fermenting distiller's grain water comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein butyric acid fermentation thalli is inoculated in a seed tank culture medium for seed culture and then inoculated in fermentationCarrying out fermentation culture in a tank culture medium, wherein the fermentation tank culture medium comprises the following components: 50-200 g/L of wine tank water, 2-5 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water.

Further, the fermenter medium composition comprises: 130-150g/L of wine tank water, 2-5 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water.

Further, the composition of the seeding tank medium comprises: glucose 20g/L, yeast extract 2g/L, peptone 4g/L, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water.

The invention has the advantages that:

according to the method for producing butyric acid by using the vinasse water for fermentation, provided by the invention, the carbon source glucose and the organic nitrogen source in the fermentation medium are partially replaced by the vinasse water on the basis of the culture medium, so that the environmental protection problem can be solved, the waste is changed into valuable, the use cost of butyric acid fermentation raw materials can be reduced, and the method has higher economic benefit, environmental protection benefit and social benefit.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the specific examples described herein are only for explaining the present invention and are not intended to limit the present invention.

Example 1

A method for producing butyric acid by fermenting distiller's grain water comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein the butyric acid fermentation thalli is inoculated in a seed tank culture medium for seed culture, and the seed tank culture medium comprises the following components: glucose 20g/L, yeast extract 2g/L, peptone 4g/L, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. Then inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the fermentation tank culture medium comprises the following components: 50-60 g/L of wine tank water, 2-5 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. And (3) respectively detecting total sugar, reducing sugar and amino nitrogen after the culture medium is sterilized, and respectively determining the content of butyric acid after the fermentation is finished.

Example 2

A method for producing butyric acid by fermenting distiller's grain water comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein the butyric acid fermentation thalli is inoculated in a seed tank culture medium for seed culture, and the seed tank culture medium comprises the following components: 20g/L of glucose, 2g/L of yeast extract, 4g/L of peptone,(NH₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. Then inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the fermentation tank culture medium comprises the following components: 90-120 g/L of wine tank water, 2-5 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. And (3) respectively detecting total sugar, reducing sugar and amino nitrogen after the culture medium is sterilized, and respectively determining the content of butyric acid after the fermentation is finished.

Example 3

A method for producing butyric acid by fermenting distiller's grain water comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein the butyric acid fermentation thalli is inoculated in a seed tank culture medium for seed culture, and the seed tank culture medium comprises the following components: glucose 20g/L, yeast extract 2g/L, peptone 4g/L, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. Then inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the fermentation tank culture medium comprises the following components: 130-150g/L of wine tank water, 2-5 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. And (3) respectively detecting total sugar, reducing sugar and amino nitrogen after the culture medium is sterilized, and respectively determining the content of butyric acid after the fermentation is finished.

Example 4

A method for producing butyric acid by fermenting distiller's grain water comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein the butyric acid fermentation thalli is inoculated in a seed tank culture medium for seed culture, and the seed tank culture medium comprises the following components: glucose 20g/L, yeast extract 2g/L, peptone 4g/L, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. Then inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the fermentation tank culture medium comprises the following components: 160-200 g/L of wine tank water, 2-5 g/L of organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. And (3) respectively detecting total sugar, reducing sugar and amino nitrogen after the culture medium is sterilized, and respectively determining the content of butyric acid after the fermentation is finished.

Comparative example

A method for producing butyric acid by fermenting distiller's grain water comprises the following steps: the fermentation production method comprises seed culture and fermentation culture, wherein butyric acid fermentation thalli is inoculated to a seed tank culture medium for seedCulturing, wherein the composition of a seeding tank culture medium comprises: glucose 20g/L, yeast extract 2g/L, peptone 4g/L, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. Then inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the fermentation tank culture medium comprises the following components: 50-60 g/L glucose, 2-8 g/L organic nitrogen source, (NH)₄)₂SO₄ 2g/L、K₂HPO₄ 1g/L、KH₂PO₄ 1g/L、MgSO₄·7H₂O 0.1g/L、FeSO₄·7H₂O 0.015g/L、CaCl₂·2H₂O 0.015g/L、MnSO₄·H₂O 0.01g/L、CoCl₂·6H₂O 0.02g/L、ZnSO₄·7H₂O0.002 g/L, and the balance being water. And (3) respectively detecting total sugar, reducing sugar and amino nitrogen after the culture medium is sterilized, and respectively determining the content of butyric acid after the fermentation is finished.

Comparison of the effects of examples and comparative examples

The fermentation period of the comparative example is 48-52h, and the content of butyric acid is 47-55 g/L; the fermentation period of the embodiment 1 is 48-52h, and the content of butyric acid is 47-51 g/L; the fermentation period of the embodiment 2 is 48-51h, and the content of butyric acid is 47-53 g/L; the fermentation period of the embodiment 3 is 48-51h, and the content of butyric acid is 48-54 g/L; the fermentation period of example 4 is 48-51h, and the butyric acid content is 48-55 g/L.

From the above results, it can be seen that the production of butyric acid by fermentation of the stillage water is increased in the amount of 130-150g/L because the content of sugar in the stillage water is slightly low. Because the sugar content of the wine tank water is low, the wine tank water can be used after being made into saccharified wine tank water through saccharification treatment, and the using amount of the wine tank water can be reduced.