Composition for promoting differentiation or proliferation of human adipose-derived stem cells

文档序号：788136 发布日期：2021-04-09 浏览：21次中文

阅读说明：本技术 用于促进人脂肪干细胞的分化或增殖的组合物 (Composition for promoting differentiation or proliferation of human adipose-derived stem cells ) 是由李善珠金米娜金美善于 2019-08-23 设计创作，主要内容包括：本发明涉及包含选自由芒柄花黄素、大黄素、阿特匹林C、鼠尾草酸、棕榈酰胺、愈创木薁磺酸钠、咖啡酸、乙酰基扁桃酸、紫胶酸、泛醌、羟基肉桂酸、叶黄素类、覆盆子酮、没食子酸、松油醇、甘露糖磷酸酯钠、托酚酮、甘草次酸、羊毛甾醇、核黄素磷酸酯钠、甲萘醌、硫辛酸、乳黄素、洋葱提取物、米糠提取物、竹子提取物、胡萝卜提取物、木槿提取物、厚朴提取物、芦荟提取物以及提取物的馏分物构成的组中的任意一种以上的物质作为有效成分的用于增加皮肤弹性及改善皱纹的化妆材料组合物、食品组合物、药物组合物、医药外品组合物、用于促进源自人脂肪的干细胞的分化或增殖的组合物及包括将所述组合物投给除了人以外的个体的步骤的增加皮肤弹性的方法。(The present invention relates to a cosmetic material composition, a food composition, a pharmaceutical external composition, a composition for promoting differentiation or proliferation of human fat-derived stem cells, comprising, as an active ingredient, any one or more selected from the group consisting of formononetin, emodin, aplidine C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acids, xanthophylls, raspberry ketones, gallic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin phosphate, menadione, lipoic acid, lactoflavin, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and a fraction of the extract, for increasing skin elasticity and improving wrinkles, and a cosmetic composition, a food composition, a pharmaceutical external composition, a composition for promoting differentiation or proliferation of human fat-derived stem cells, and a composition comprising a step of administering the composition to a subject other than a human The method of increasing skin elasticity.)

1. A cosmetic material composition for increasing skin elasticity and improving wrinkles, wherein,

contains as an active ingredient one or more substances selected from the group consisting of formononetin, emodin, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, raspberry ketone, gallic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin phosphate sodium, menadione, lipoic acid, lactoflavin, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and distillate of the above extracts.

2. The cosmetic material composition according to claim 1,

comprises formononetin, emodin, Abelipin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, raspberry ketone, gallic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, lipoic acid, lactoflavin or xanthophylls as an active ingredient.

3. The cosmetic material composition according to claim 1,

the substance is contained in an amount of 0.0001 to 10% by weight relative to the total weight of the composition.

4. The cosmetic material composition according to claim 1,

the extract is extracted with water, C1-C6 alcohol or their mixture.

5. The cosmetic material composition according to claim 1,

the substance promotes differentiation of human adipose-derived stem cells into adipocytes.

6. The cosmetic material composition according to claim 5,

the substance is one or more substances selected from the group consisting of formononetin, emodin, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, magnolia bark extract, aloe extract, a fraction of magnolia bark extract, and a fraction of aloe extract.

7. The cosmetic material composition according to claim 1,

the substance promotes proliferation of stem cells derived from human fat.

8. The cosmetic material composition according to claim 7,

the substance is one or more selected from the group consisting of raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin phosphate sodium, menadione, lipoic acid, lactoflavin, xanthophylls, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, and a fraction of the above extract.

9. The cosmetic material composition according to claim 1,

the composition is in the form of soft lotion, nourishing lotion, astringent lotion, nourishing cream, massage cream, essence, facial mask, skin patch, skin gel, powder, ointment, suspension, emulsion, spray or skin caring liquid.

10. A food composition for increasing skin elasticity and improving wrinkles, wherein,

11. The food composition of claim 10,

12. A pharmaceutical composition for increasing skin elasticity and improving wrinkles, wherein,

13. The pharmaceutical composition of claim 12,

14. A pharmaceutical composition for external use for increasing skin elasticity and improving wrinkles, wherein,

contains as an active ingredient one or more substances selected from the group consisting of formononetin, rhubarb, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, raspberry ketone, gallic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, lipoic acid, lactoflavin, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and distillate of the above extracts.

15. The pharmaceutical topical composition of claim 14,

16. A composition for promoting differentiation of stem cells derived from human fat, wherein,

comprises as an active ingredient one or more substances selected from the group consisting of formononetin, emodin, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, magnolia bark extract, aloe extract, a fraction of magnolia bark extract and a fraction of aloe extract.

17. A composition for promoting proliferation of stem cells derived from human fat, wherein,

comprises one or more substances selected from the group consisting of raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin phosphate sodium, menadione, lipoic acid, lactoflavin, xanthophylls, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract and a fraction of the above extract as an active ingredient.

18. A method for increasing the elasticity of skin, wherein,

comprising the step of administering to an individual other than human a composition containing as an active ingredient one or more selected from the group consisting of formononetin, emodin, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, raspberry ketone, gallic acid, terpineol, sodium mannosephosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin phosphate sodium, menadione, lipoic acid, lactoflavin, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and distillates of the extracts.

19. A cosmetic composition for increasing elasticity of lip skin and improving wrinkles, wherein,

20. The cosmetic material composition according to claim 19,

the active ingredient of the cosmetic composition for increasing the elasticity of lip skin and improving wrinkles comprises one or more substances selected from the group consisting of magnolia bark extract and a fraction of magnolia bark extract.

21. The cosmetic material composition according to claim 19,

the cosmetic composition for lip skin is in a form selected from lipstick, lip gloss, or lip balm.

Technical Field

The present invention relates to a cosmetic composition comprising a compound selected from the group consisting of Formononetin (Formononetin), Emodin (Emodin), aprepilin C (artepilin C), Carnosic Acid (Carnosic Acid), Palmitamide (Palmitamide), Sodium Guaiazulene Sulfonate (Sodium Guaiazulene Sulfonate), Caffeic Acid (Caffeic Acid), Acetyl Mandelic Acid (Acetyl Mandelic Acid), Laccaic Acid (Laccaic Acid), Ubiquinone (Ubiquinone), Hydroxycinnamic Acid (Hydroxycinnamic Acid), xanthophylls (Xanthophyll), Raspberry ketone (Raspberry ketone), gallic Acid (gallic Acid), terpineol (terpineol), Sodium mannose phosphate (Sodium glycerophosphate), tropolone (troquinone), glycyrrhetinic Acid (glycerol), lanolin ester (rice bran), lanolin ester extract (Sodium lactate), Sodium onion extract (vitamin E), vitamin E (vitamin E) extract (vitamin E), vitamin E (vitamin E) extract (vitamin E), vitamin E (vitamin E) extract (vitamin E), vitamin E (vitamin E) and vitamin E (vitamin E) extract (vitamin E) and vitamin E (vitamin E) and, A cosmetic material composition for increasing skin elasticity and improving wrinkles, a food composition, a pharmaceutical external composition, a composition for promoting differentiation or proliferation of stem cells derived from human fat, comprising as an active ingredient one or more substances selected from the group consisting of a hibiscus extract, a magnolia bark extract, an aloe extract, and a fraction of the extract, and a method for increasing skin elasticity comprising the step of administering the composition to an individual other than a human.

Background

Adipose tissue, together with muscle, is responsible for the supporting role of the skin structure and is an important cell in the skin structure, accounting for around 90% by volume although the number of adipose cells is only 30 to 40% by number. It is known that subcutaneous adipose tissue is reduced as an individual ages, whereby the elasticity of the skin is reduced and many wrinkles are generated. In addition, when fat is reduced due to rapid weight loss, a decrease in facial elasticity and an increase in wrinkles may be frequently observed. Therefore, increasing the volume in the skin by adjusting the amount of adipose tissue that plays an important role in maintaining the structure in the skin may be a way to improve skin elasticity and wrinkles. However, in the case of the current fat transplantation surgery for improving the elasticity of the skin, the following problems occur: the survival rate of transplanted stem cells in vivo is very low, and it is necessary to perform a re-operation after a certain time, the transplanted fat is agglomerated, and the like. Therefore, it is necessary to study a substance capable of enhancing the structure of the subcutaneous fat layer without such a surgery.

However, in the case of the conventional technique of directly injecting adipose-derived stem cells, there is a possibility that a safety problem occurs, and in the case of the technique of mimicking components secreted during cell culture and providing the same to the skin, there is a limitation that it is difficult to mimic the components according to actual components. In addition, the cell activity of human adipose-derived stem cells is decreased as the skin is aged, so that the time required for proliferation is increased more than two times (Gerontolog 2011; 57:66-75), and thus there is a problem in that the reactivity of adipose-derived stem cells present in aging skin is greatly reduced relative to other application techniques. Therefore, it is necessary to study a substance capable of activating subcutaneous adipose tissue by promoting the proliferation of adipose stem cells while not irritating the skin.

In addition, in the skin, the lips are exposed to extreme environments in addition to the most moving parts of the body parts due to repeated contraction and expansion, and thus are easily affected by environmental factors such as ultraviolet rays, cold, dryness, and the like. Furthermore, the lips have weak self-defense ability due to their structure unlike general skin, and their volume is reduced with aging, wrinkles are increased, and thus attractive shapes are lost. Under the circumstances, there is a need to develop a composition having excellent effects on volume and wrinkle care on the lips as in the case of the general skin, but there are only techniques of directly using a wrinkle-improving component studied on the face skin, and techniques of temporarily increasing the volume. The latter volume-increasing phenomenon has a limitation in causing a stimulatory response (Korean laid-open publication No. 10-2012-0140450A).

In recent years, in studies related to adipocytes or fat transplantation, adipose-derived stem cells that are present in subcutaneous adipose tissue and have the ability to differentiate into adipocytes have received much attention. This is because it is known that adipocytokine (adipokine) secreted from adipose-derived stem cells not only increases collagen synthesis in the dermis but also affects skin regeneration. In the case of known substances that differentiate existing adipocyte precursor cells into adipocytes, the efficiency is relatively low when applied to human adipose-derived stem cells, since they are the result of targeting murine adipocyte precursor cells.

Disclosure of Invention

Problems to be solved by the invention

The present inventors have made intensive efforts to find a substance capable of effectively promoting differentiation of fat-derived stem cells into adipocytes or proliferation of fat-derived stem cells, and as a substance capable of promoting the process of differentiation of human fat-derived stem cells into adipocytes, Formononetin (Formononetin), Emodin (Emodin), apigenin c (artepilin c), Carnosic Acid (Carnosic Acid), palmamide (Palmitamide), Sodium guaazulene Sulfonate (Sodium Guaiazulene Sulfonate), Caffeic Acid (Caffeic Acid), Acetyl Mandelic Acid (Acetyl manic Acid), Laccaic Acid (Laccaic Acid), Ubiquinone (ubine), hydroxyhydroxyiminoctamine Acid), xanthophylls (xanthophylls), Raspberry ketone (Raspberry ketone), gallic Acid (gallic Acid ester), pinolequinone (hydroquinone), Sodium phospho (Sodium phospho Acid), and so on have been developed, Glycyrrhetinic acid (glycyrrhetic acid), lanosterol (lanosterol), riboflavin phosphate sodium (sodium riboflavin phosphate), menadione (menadione), lipoic acid (thioctic acid), lactoflavin (lactoflavin), onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, or aloe extract, and it was confirmed that the substances can be used for the purpose of increasing skin elasticity and improving wrinkles, thereby completing the present invention.

Technical scheme for solving problems

It is an object of the present invention to provide a cosmetic composition comprising a compound selected from the group consisting of Formononetin (Formononetin), Emodin (Emodin), atopirin C (artepilin C), Carnosic Acid (Carnosic Acid), Palmitamide (Palmitamide), Sodium guaazulene Sulfonate (Sodium Guaiazulene Sulfonate), Caffeic Acid (Caffeic Acid), Acetyl Mandelic Acid (Acetyl Mandelic Acid), Laccaic Acid (Laccaic Acid), Ubiquinone (ubine), Hydroxycinnamic Acid (Hydroxycinnamic Acid), xanthophylls (xanthophoyl), Raspberry ketone (Raspberry ketone), gallic Acid (gallic Acid), terpineol (terpineol), Sodium mannose phosphate (Sodium phosphate), tropolone (troquinone), glycyrrhetinic Acid (glycerol), glycolic Acid (glycerol acetate), lignan extract (Sodium ascophyll), naphthoquinone (Sodium phosphate), naphthoquinone (Sodium xanthophylline phosphate), naphthoquinone (Sodium naphthoquinone extract (naphthoquinone), or naphthoquinone (naphthoquinone extract (Sodium naphthoquinone), or naphthoquinone (Sodium naphthoquinone, naphthoquinone extract (Sodium naphthoquinone, or a naphthoquinone extract (naphthoquinone), or a, A cosmetic material composition for increasing skin elasticity and improving wrinkles, comprising as an active ingredient one or more selected from the group consisting of a carrot extract, a hibiscus extract, a magnolia bark extract, an aloe extract and a distillate of the extracts.

Another object of the present invention is to provide a food composition for increasing skin elasticity and improving wrinkles, comprising as an active ingredient one or more selected from the group consisting of formononetin, emodin, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, magnolia bark extract, aloe vera extract, a fraction of magnolia bark extract, and a fraction of aloe vera extract.

It is another object of the present invention to provide a pharmaceutical composition comprising a compound selected from the group consisting of formononetin, emodin, atopium C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, Raspberry ketones (Raspberry ketone), gallic acid (gallic acid), terpineol (terpineol), a pharmaceutical composition for increasing skin elasticity and improving wrinkles, comprising as an active ingredient one or more selected from the group consisting of sodium mannose phosphate (sodium mannose phosphate), tropolone (tropilone), glycyrrhetinic acid (glycyrrhetic acid), lanosterol (lanosterol), sodium riboflavin phosphate (sodium riboflavin phosphate), menadione (menadione), lipoic acid (thioctic acid), lactoflavin (lactoflavin), onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and fractions of said extracts.

It is another object of the present invention to provide a pharmaceutical composition comprising a compound selected from the group consisting of formononetin, emodin, atopium C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, Raspberry ketones (Raspberry ketone), gallic acid (gallic acid), terpineol (terpineol), a pharmaceutical external composition for increasing skin elasticity and improving wrinkles, comprising as an active ingredient one or more selected from the group consisting of sodium mannose phosphate (sodium mannose phosphate), tropolone (tropolone), glycyrrhetinic acid (glycyrrhetic acid), lanosterol (lanosterol), sodium riboflavin phosphate (sodium riboflavin phosphate), menadione (menadione), lipoic acid (thioctic acid), lactoflavin (lactoflavin), onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and fractions of the above extracts.

It is still another object of the present invention to provide a pharmaceutical composition comprising a compound selected from the group consisting of formononetin, emodin, atopium C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, Raspberry ketone (Raspberry ketone), gallic acid (gallic acid), terpineol (terpineol), a composition for promoting differentiation of human fat-derived stem cells, comprising as an active ingredient one or more members of the group consisting of sodium mannose phosphate (sodium mannose phosphate), tropolone (tropilone), glycyrrhetinic acid (glycyrrhetic acid), lanosterol (lanosterol), sodium riboflavin phosphate (sodium riboflavin phosphate), menadione (menadione), lipoic acid (thioctic acid), lactoflavin (lactoflavin), onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and fractions of said extracts.

It is still another object of the present invention to provide a method for increasing skin elasticity comprising the step of administering the composition to an individual other than a human.

Effects of the invention

The composition of the present invention can be used for the purpose of anti-aging by enhancing the structure of subcutaneous adipose layers, increasing the elasticity of the skin, and improving wrinkles by promoting the differentiation of human adipose-derived stem cells into adipocytes or promoting the proliferation of human adipose-derived stem cells.

Drawings

FIG. 1 shows the results of staining lipids in differentiated adipocytes with oil red O after treating stem cells derived from human fat with formononetin, acetyl mandelic acid, and Magnolia bark extract.

Figure 2 quantitatively shows the relative differentiation rates of human fat derived stem cells treated with formononetin, emodin, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, magnolia bark extract, and aloe vera extract.

FIG. 3 shows the results of comparison of those who improved canthus wrinkles and lip wrinkles with Magnolia bark extract.

Figure 4a shows the persistence of lip wrinkle improvement up to week 12 for subject 1 when treated with magnolia bark extract, and figure 4b shows the persistence of lip wrinkle improvement up to week 12 for subject 2.

FIG. 5 shows the results of comparing the wrinkle improvement rates of the canthus and lip of Magnolia officinalis extract and asiaticoside as a conventional wrinkle improvement material.

Fig. 6 shows the cell activity rates in human fat-derived stem cells treated with raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, lipoic acid, lactoflavin, xanthophylls, onion, rice bran, bamboo, carrot, and hibiscus extract.

Detailed Description

This will be specifically explained below. Meanwhile, each of the descriptions and embodiments disclosed in the present application can also be applied to each of the other descriptions and embodiments. That is, all combinations of the various elements disclosed in the present application are within the scope of the present application. In addition, the scope of the present application should not be considered limited to the specific description set forth below.

In one aspect of the present invention, there is provided a cosmetic composition comprising a compound selected from the group consisting of Formononetin (Formononetin), Emodin (Emodin), atopizin C (artepilin C), Carnosic Acid (Carnosic Acid), Palmitamide (Palmitamide), Sodium guaazulene Sulfonate (Sodium Guaiazulene Sulfonate), Caffeic Acid (Caffeic Acid), Acetyl Mandelic Acid (Acetyl Mandelic Acid), Laccaic Acid (Laccaic Acid), Ubiquinone (ubine), Hydroxycinnamic Acid (Hydroxycinnamic Acid), xanthophylls (Xanthophyll), Raspberry ketone (Raspberry ketone), gallic Acid (gallic Acid), terpineol (terpineol), Sodium mannose phosphate (Sodium glycerophosphate), tropolone (trophenol), glycyrrhetin (glycolic Acid), lanolin fatty Acid (saponin), lanolin fatty Acid (Sodium lactate), Sodium onion flavonol (Sodium lactate), Sodium onion lecithin (Sodium lactate), vitamin E (vitamin E), vitamin E (vitamin E, vitamin E, A cosmetic material composition for increasing skin elasticity and improving wrinkles, comprising as an active ingredient one or more substances selected from the group consisting of hibiscus extract, magnolia bark extract, aloe extract and a fraction of the extract. The composition may comprise 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or 16 of the substances as an effective ingredient.

In addition, another embodiment of the present invention provides a cosmetic material composition for increasing skin elasticity and improving wrinkles, comprising, as an effective ingredient, a cosmetically acceptable salt of formononetin, emodin, atopirine C, carnosic acid, palmamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, raspberry ketone, gallic acid, terpineol, sodium mannosyl phosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin phosphate sodium, menadione, lipoic acid, lactoflavin, or xanthophylls.

In the present invention, the "cosmetically acceptable salt" is in the form of a salt of formononetin, emodin, atopirine C, carnosic acid, palmamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, raspberry ketone, gallic acid, terpineol, sodium mannosephosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin phosphate sodium, menadione, lipoic acid, lactoflavin, or xanthophyll in combination with other substances, and means a substance capable of exhibiting an activity similar to that of the compound cosmetically acceptable, but is not limited thereto.

As a result of diligent efforts to develop a substance capable of activating and restoring an aged subcutaneous adipose layer, the present inventors developed the following substances capable of promoting differentiation of human adipose-derived stem cells into adipocytes and proliferation of human adipose-derived stem cells: formononetin, emodin, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acids, xanthophylls, Raspberry ketone (Raspberry ketone), gallic acid (gallic acid), terpineol (terpineol), sodium mannose phosphate (sodium mannose phosphate), tropolone (tropolone), glycyrrhetinic acid (glycyrrhetic acid), lanosterol (lanosterol), sodium riboflavin phosphate (sodium riboflavacin phosphate), menadione (menadione), thioctic acid (thioctic acid), lactoflavin (lactoflavin), onion extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, and aloe extract. It has thus been found that a composition comprising the substance can be used as a cosmetic material composition for increasing skin elasticity and improving wrinkles. The effect of the substance on increasing skin elasticity and improving wrinkles has not been known at all so far, and is very significant from the aspect originally developed by the present inventors.

The molecular formula of the term "Formononetin (Formononetin)" of the invention is C₁₆H₁₂O₄Molecular weight is 284.26, and can be represented by the following chemical formula 1.

[ chemical formula 1]

Formononetin of the present invention can be isolated from plants including licorice, but is not limited to its obtaining method, and a chemically synthesized substance by a method known in the art may be used, or a commercially available substance may be used.

The term "Emodin" according to the present invention has the molecular formula C₁₅H₁₀O₅The molecular weight is 270.24, and can be represented by the following chemical formula 2.

[ chemical formula 2]

Emodin of the present invention is not limited to the method for obtaining it, and chemically synthesized by a method known in the art may be used, or commercially available substances may be used.

The term "Abelilin C (artemillin C)" according to the invention has the formula C₁₉H₂₄O₃Molecular weight is 300.40, and can be represented by the following chemical formula 3.

[ chemical formula 3]

The method for obtaining the Abiprin C of the present invention is not limited to the above method, and a chemically synthesized substance by a method known in the art may be used, or a commercially available substance may be used.

The term Carnosic acid according to the present invention has the formula C₂₀H₂₈O₄Molecular weight is 332.44, and can be represented by the following chemical formula 4.

[ chemical formula 4]

The carnosic acid of the present invention is not limited to the method for obtaining it, and a chemically synthesized carnosic acid by a method known in the art may be used, or a commercially available carnosic acid may be used.

The term "Palmitamide" according to the invention may be a C16 linear fatty acid amide of formula C₁₆H₃₃NO, having a molecular weight of 255, can be represented by the following chemical formula 5.

[ chemical formula 5]

The method for obtaining the palmitamide of the present invention is not limited to the method, and a chemically synthesized one by a method known in the art may be used, or a commercially available one may be used.

The molecular formula of the term "Sodium Guaiazulene Sulfonate (Sodium Guaiazulene Sulfonate)" in the invention is C₁₅H₁₇NaO₃S, molecular weight of 300.35, can be represented by the following chemical formula 6.

[ chemical formula 6]

The sodium guaiazulene sulfonate of the present invention is not limited to the method for obtaining it, and a chemically synthesized substance by a method known in the art may be used, or a commercially available substance may be used.

The term Caffeic Acid (Caffeic Acid) according to the present invention has the molecular formula C₉H₈O₄The molecular weight is 180.157, and can be represented by the following chemical formula 7.

[ chemical formula 7]

The caffeic acid of the present invention is not limited to the method for obtaining it, and a chemically synthesized one by a method known in the art may be used, or a commercially available one may be used.

Specifically, the term "Acetyl Mandelic Acid" in the present invention may be "O-Acetyl Mandelic Acid" having a molecular formula of C₁₀H₁₀O₄The molecular weight is 194.186, and can be represented by the following chemical formula 8.

[ chemical formula 8]

Acetyl mandelic acid of the present invention is not limited to the method for obtaining it, and a chemically synthesized substance by a method known in the art may be used, or a commercially available substance may be used.

The term "Laccaic Acid" of the present invention is a kind of natural anthraquinone, which is a dark red crystal made by coagulating secretions of lacca Kerr (Laccifer lacca Kerr), and is well known to be mainly used as a dye. The lac acid may be lac acid a, lac acid B, lac acid C, lac acid D, lac acid E, or lac acid F, but is not particularly limited thereto. In one embodiment of the present invention, 50506(Natural red 25) from Sigma-Aldrich is used as the lac acid.

The laccaic acid of the present invention is not limited to the method for obtaining the laccaic acid, and a chemically synthesized laccaic acid obtained by a method known in the art may be used, or a commercially available laccaic acid may be used.

The term "Ubiquinone (Ubiquinone)" of the present invention is one of fat-soluble quinones, which are constituent factors of the electron transport system of mitochondria. Specifically, the ubiquinone may be ubiquinone (10), but is not limited thereto, in which case it has the formula C₅₉H₉₀O₄Molecule(s)The amount is 863.37 and can be represented by the following chemical formula 9.

[ chemical formula 9]

The ubiquinone of the present invention is not limited to the method for obtaining the ubiquinone, and a chemically synthesized material by a method known in the art may be used, or a commercially available material may be used.

The molecular formula of the term 'Hydroxycinnamic Acid' in the invention is C₉H₈O₃The molecular weight is 164.158, and both isomers (o-, m-, p-hydroxycinnamic acid: o-, m-, p-hydroxycinnamic acid) and isomers (cis-Coumaric acid (Coumaric acid) and trans-Coumaric acid (Coumaric acid)) which differ depending on the position of the-OH group directly substituted on the benzene ring, specifically, m-Coumaric acid (m-Coumaric acid) may be included, but not particularly limited thereto. The hydroxycinnamic acid may be represented by the following chemical formula 10.

[ chemical formula 10]

The hydroxycinnamic acid of the present invention is not limited to the method for obtaining it, and a chemically synthesized one by a method known in the art may be used, or a commercially available one may be used.

The term "xanthophylls (xanthophylls)" of the present invention is an oxyprotenoid pigment of carotenoids present in green parts of plants such as leaves, flowers and fruits, which is expressed in yellow color, and has a molecular formula of C₄₀H₅₆O₂Molecular weight is 569. Specifically, the xanthophylls may be lutein (lutein), and may be represented by the following chemical formula 11.

[ chemical formula 11]

The xanthophylls or xanthophylls of the present invention are not limited to the method for obtaining them, and those chemically synthesized by a method known in the art may be used, or commercially available ones may be used.

The molecular formula of the term Raspberry ketone (Raspberry ketone) is C₁₀H₁₂O₂Molecular weight of 164.20, which can be represented by the following chemical formula 12.

[ chemical formula 12]

The raspberry ketone of the present invention is not limited to the method for obtaining it, and a chemically synthesized material by a method known in the art may be used, or a commercially available material may be used.

The term "gallic acid" according to the present invention has the molecular formula C₇H₆O₅Molecular weight of 170.12, which can be represented by the following chemical formula 13.

[ chemical formula 13]

The gallic acid of the present invention is not limited to the method for obtaining it, and a chemically synthesized gallic acid by a method known in the art may be used, or a commercially available gallic acid may be used.

The term "terpineol" according to the present invention has the molecular formula C₁₀H₁₈O, molecular weight of 154, can be represented by the following chemical formula 14.

[ chemical formula 14]

The terpineol of the present invention is not limited to the method for obtaining it, and a chemically synthesized one by a method known in the art may be used, or a commercially available one may be used.

The term "sodium mannose phosphate" according to the invention has the molecular formula C₆H₁₂O₉PNa, molecular weight 282.12. Specifically, the Mannose phosphate sodium may be Mannose 6-phosphate sodium or D-Mannose 6-phosphate sodium salt (D-Mannose-6-phosphate salt), but is not limited thereto, and may be represented by the following chemical formula 15.

[ chemical formula 15]

The mannose phosphate sodium of the present invention is not limited to the method for obtaining it, and a chemically synthesized material by a method known in the art may be used, or a commercially available material may be used.

The term "tropolone" according to the invention has the formula C₇H₆O₂The molecular weight is 122, which can be represented by the following chemical formula 16.

[ chemical formula 16]

The tropolone of the present invention is not limited to the method for obtaining it, and a chemically synthesized substance by a method known in the art may be used, or a commercially available substance may be used.

The term "glycyrrhetic acid" of the present invention has the molecular formula C₃₀H₄₆O₄The molecular weight is 471, which can be represented by the following chemical formula 17.

[ chemical formula 17]

The glycyrrhetinic acid of the present invention is not limited to the method for obtaining it, and a chemically synthesized one by a method known in the art may be used, or a commercially available one may be used.

The term "lanosterol" according to the invention has the molecular formula C₃₀H₅₀O, molecular weight of 427, can be represented by the following chemical formula 18.

[ chemical formula 18]

The lanosterol of the present invention is not limited to the method for obtaining it, and a chemically synthesized substance by a method known in the art may be used, or a commercially available substance may be used.

The term "sodium riboflavin phosphate" according to the present invention has the molecular formula C₁₇H₂₀N₄NaO₉P, molecular weight of 478.33, can be represented by the following chemical formula 19.

[ chemical formula 19]

The riboflavin phosphate sodium of the present invention is not limited to the method for obtaining it, and a chemically synthesized substance by a method known in the art may be used, or a commercially available substance may be used.

The term "menadione" of the present invention is a yellow crystal as one of vitamin K, and its molecular formula is C₁₁H₈O₂Molecular weight of 172.18, and can be represented by the following chemical formula 20.

[ chemical formula 20]

Menadione of the present invention is not limited to the method for obtaining menadione, and a chemically synthesized menadione by a method known in the art may be used, or a commercially available menadione may be used.

The term "lipoic acid" according to the invention, also known as lipoid acid (lipoid), has the formula C₈H₁₄O₂S₂The molecular weight is 206, and can be represented by the following chemical formula 21.

[ chemical formula 21]

The lipoic acid or lipoid acid of the present invention is not limited to the method for obtaining it, and a chemically synthesized substance by a method known in the art may be used, or a commercially available substance may be used.

The term "lactoflavin" in the present invention refers to riboflavin (riboflavin, vitamin B) isolated from milk₂(vitamin B₂)). The molecular formula of the lactoflavin is C₁₇H₂₀N₄O₆Molecular weight of 376.36, and can be represented by the following chemical formula 22.

[ chemical formula 22]

The lactoflavin of the present invention is not limited to the method for obtaining it, and a chemically synthesized substance by a method known in the art may be used, or a commercially available substance may be used.

The formononetin, emodin, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, raspberry ketone, gallic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menadione, lipoic acid, lactoflavin or xanthophylls may be present not only in solvated form but also in unsolvated form. In addition, the compounds may exist in crystalline or amorphous form, and all such physical forms are included within the scope of the present invention.

In addition, it is apparent that the scope of the compound includes not only the compound but also a cosmetically acceptable salt, a dietetically acceptable salt, and a pharmaceutically acceptable salt thereof.

The term "onion (onion )" of the present invention is a two-year-old herb of liliaceae, order liliaceae, a monocotyledonous plant, and is known to contain quercetin, chromium, allicin and the like as main components and to have anti-inflammatory, blood sugar regulating and blood pressure lowering effects. The onion may be purchased from commercial sale, may be extracted from nature or cultivated, and may be used without limitation to roots, stems, leaves, etc.

The term "rice bran (rice bran)" of the present invention refers to a pulverized mixture of pericarp, seed coat, aleurone layer, etc. generated when milling brown rice into polished rice, and it is known that inorganic substances such as vitamin B1, B2, nicotinic acid, etc. and oryzanol, tocopherol, phytic acid, etc. are contained to reduce cholesterol in blood, to prevent oxidation, to prevent cancer, etc.

The term "bamboo" of the present invention is an evergreen perennial plant belonging to the subfamily Bambusoideae of the order gramineae of monocotyledonous plants, and is well known in the field of anti-oxidation, bactericidal, anti-cancer effects and the like in traditional Chinese medicine. The bamboo may be purchased from commercial sale, may be extracted from nature or cultivated, and may be used without limitation to roots, stems, leaves, etc.

The term "carrot (carrot) is a two-year-old herbaceous plant of the Umbelliferae family of the order Umbelliferae, dicotyledons, and is known to contain β -carotene, lycopene, and the like as main components to have antioxidant, anti-aging, and immunity-enhancing effects. The carrot may be used without limitation as purchased from commercial sale, as extracted from nature or cultivated, and as a root, stem, leaf, etc. may be used without limitation.

The term "Hibiscus (Rose of China, Hibiscus)" of the present invention refers to a plant belonging to the genus Hibiscus of the family Malvaceae, the order Malvales, dicotyledonous plants, and is known to be rich in Hibiscus acid, hydroxycitric acid, etc. for its effect of preventing obesity, etc. The hibiscus syriacus may be used without limitation as purchased from commercial sale, may be used as extracted from nature or cultivated, and may be used without limitation as a root, stem, leaf, etc.

The term "magnolia officinalis (magnolia Cortex)" of the present invention is a dried stem or root bark of magnolia officinalis tree of magnolia family, which contains lignan and essential oil components as main active ingredients. Magnolia officinalis is known to have anti-inflammatory, analgesic, smooth muscle regulating and antiulcer effects. The Magnolia officinalis can be purchased from commercial sale without limitation, or extracted from nature or cultivated.

The term "Aloe vera" of the present invention is a perennial plant belonging to the genus Aloe of the family liliaceae, also known as Aloe vera. The aloe contains aloin, aloe-emodin, aloe tincture, aloe miso, etc. as main active ingredients, and is well known for its antibacterial, antifungal, antitumor, etc. effects. The aloe can be used without limitation as purchased from commercial sale, or as extracted from nature or cultivated.

The term "extract" in the present invention refers to a liquid-phase component obtained by immersing a target substance in various solvents and then extracting the resultant product at normal temperature, low temperature or heated state for a predetermined time, and a product such as a solid powder obtained by removing the solvent from the liquid-phase component. Furthermore, in addition to the products, they may be openly construed to include all dilutions of the products, concentrates of the products, crude and purified products of the products, and the like.

In the present invention, the extract may be an extract of onion, rice bran, bamboo, carrot, hibiscus, magnolia bark or aloe. The extract may be extracted from various organs of natural plants, hybrid plants, and variant plants, for example, not only from roots, aerial parts, stems, leaves, flowers, trunk of fruits, epidermis of fruits, but also from plant tissues. The extract can be obtained by extraction using water or various organic solvents, or the like. At this time, the organic solvent used is not particularly limited as long as an extract can be obtained, and specifically, may be water, a polar solvent or a nonpolar solvent, more specifically, may be water, a lower alcohol having 1 to 6 carbon atoms (methanol, ethanol, propanol, butanol, or the like), a polyhydric alcohol such as glycerin, butanediol, or the like, a hydrocarbon solvent such as methyl acetate, ethyl acetate, or the like, or a mixed solvent thereof, and more specifically, may be water, a lower alcohol, 1, 3-butanediol, or a mixed solvent thereof.

In addition, a method for obtaining the extract is not particularly limited as long as the extract of onion, rice bran, bamboo, carrot, hibiscus, magnolia bark or aloe can be obtained, and specifically, a method such as a cold-maceration extraction method of extracting at normal temperature, a heating extraction method, an ultrasonic extraction method of extracting by applying ultrasonic waves, a reflux extraction method using a reflux cooler, or the like may be used after immersing the root, stem, leaf, fruit, flower, dried products and processed products thereof, and the like of magnolia bark or aloe in the solvent.

The term "fraction" in the present invention means a product obtained by performing fractionation to separate a specific component or a specific group of components from a mixture containing various constituent components. In the present invention, the fraction may be a fraction of onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract or aloe extract.

In the present invention, the fractionation method for obtaining the fraction is not particularly limited, and may be performed according to a method generally used in the art, as long as the effects of increasing skin elasticity and improving wrinkles can be exhibited. For example, there may be mentioned a solvent fractionation method by treating various solvents, an ultrafiltration fractionation method by passing through an ultrafiltration membrane having a predetermined molecular weight cut-off, a chromatographic fractionation method by performing various chromatographies (prepared for separation depending on size, charge, hydrophobicity or hydrophilicity), a combination of these methods, and the like.

In the present invention, the type of the fractionation solvent used for obtaining the fraction is not particularly limited, and any solvent known in the art may be used. As non-limiting examples of the fractionation solvent, polar solvents such as water, distilled water, alcohols, and the like; and nonpolar solvents such as hexane (Hexan), Ethyl acetate (Ethyl acetate), Chloroform (Chloroform), Dichloromethane (dichromethane), and the like. These fractionation solvents may be used alone or two or more kinds may be mixed and used. In the case of using the alcohol in the fractionating solvent, an alcohol of C1 to C4 may be used.

In the cosmetic material composition of the present invention, 0.0001 to 10% by weight (w/w) of a fraction of one or more substances selected from the group consisting of formononetin, emodin, atopirine C, carnosic acid, palmamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acids, xanthophylls, Raspberry ketone (Raspberry ketone), gallic acid (gallic acid), terpineol (terpineol), sodium mannose phosphate (sodium mannose phosphate), tropolone (tropolone), glycyrrhetinic acid (glycyrrhetinic acid), lanosterol (lanosterol), sodium riboflavin phosphate (sodium riboflavin phosphate), menadione (menadione), lipoic acid (thioctic acid), lactoflavin (lactoflavin), onion extract, carrot extract, bamboo extract, rice bran extract, shrubalthea extract, aloe extract, and magnolia bark extract, specifically, it may include 0.0005 to 10 wt% (w/w), 0.001 to 5 wt% (w/w), 0.0001 to 2 wt% (w/w), but is not limited thereto. In the case of less than 0.0001 wt%, the effect is very small, and in the case of 2 wt% or more, a problem of generating cytotoxicity may occur.

In the present invention, the cosmetic material composition can promote the differentiation of Human adipose-derived stem cells (Human adipose-derived stem cells) into adipocytes or the proliferation of Human adipose-derived stem cells. That is, the cosmetic material composition may be a composition capable of increasing skin elasticity and improving wrinkles by promoting differentiation of human adipose-derived stem cells into adipocytes, or promoting proliferation of human adipose-derived stem cells.

The term "human adipose derived stem cell" according to the present invention is a stem cell that can be extracted from human adipose tissue, and refers to a pluripotent cell (Multipotent cell) that can be differentiated into various cells of the body. Adipose-derived stem cells are present in subcutaneous adipose tissue and have the ability to differentiate into adipocytes. It is known that adipokine (adipokine) secreted by adipose-derived stem cells not only increases collagen synthesis in the dermal layer of the skin but also affects skin regeneration.

The term "adipose cell" of the present invention refers to a cell for storing fat in adipose tissue of an individual, and particularly, may refer to white adipocyte (white adipocyte) present in a subcutaneous fat layer, but is not limited thereto.

In one embodiment of the present invention, it was confirmed that the differentiation of human fat-derived stem cells into adipocytes can be effectively promoted as a result of treating human fat-derived stem cells with formononetin, emodin, atopium C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, magnolia bark extract, and aloe extract. Therefore, the composition of the present invention comprising the substance can be used for anti-aging purposes by increasing the elasticity of the skin and improving wrinkles.

In one example of the present invention, it was confirmed that the proliferation of stem cells derived from human fat was promoted by significantly increasing the cell activity rate of cells derived from stem cells derived from human fat as a result of treating stem cells derived from human fat with an extract of raspberry ketone, gallic acid, caffeic acid, carnosic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, sodium riboflavin phosphate, menaquinone, lipoic acid, lactoflavin, xanthophylls, onion, rice bran, bamboo, carrot, and hibiscus syriacus. Therefore, the composition of the present invention comprising the substance can be used for anti-aging purposes by increasing the elasticity of the skin and improving wrinkles.

The term "increasing skin elasticity" of the present invention refers to enhancing the structure of the subcutaneous fat layer of an individual using the composition of the present invention to increase the elasticity of the skin. The skin may include any location of the skin of an individual, as long as elasticity can be increased or wrinkles can be improved with the composition of the present invention.

The term "ameliorating" of the present invention refers to the use of a composition of the present invention to alleviate the symptoms of skin wrinkles in an individual.

One aspect of the present invention provides a cosmetic material composition for increasing elasticity of lip skin and improving wrinkles, comprising as an effective ingredient one or more substances selected from the group consisting of Formononetin (Formononetin), Emodin (Emodin), apigenin c (artepilin c), Carnosic Acid (Carnosic Acid), Palmitamide (Palmitamide), Guaiazulene Sodium Sulfonate (Sodium Guaiazulene Sulfonate), Caffeic Acid (Caffeic Acid), Acetyl Mandelic Acid (Acetyl Mandelic Acid), Laccaic Acid (Laccaic Acid), Ubiquinone (ubine), Hydroxycinnamic Acid (Hydroxycinnamic Acid), xanthophylls (xanthophoyl), magnolia bark extract, aloe extract, a fraction of magnolia bark extract, and a fraction of aloe extract.

One embodiment of the present invention may be a cosmetic material composition for increasing lip skin elasticity and improving wrinkles, comprising one or more selected from the group consisting of magnolia bark extract and a fraction of magnolia bark extract.

Unlike other skin sites, the lips have no sebaceous glands and have a very thin stratum corneum. Currently, the current situation is that most of the components used for improving the volume of lips and wrinkles apply components for improving the dermal layer of skin, and the technology for improving the subcutaneous fat layer, which occupies the largest volume in the skin, has a limitation that cannot be actively applied. In addition, the technique for improving the volume of the subcutaneous fat layer is expected to be a more effective technique for improving the volume and wrinkles, because the stratum corneum of the lips is very thin and the permeation and transfer of the active ingredient are easier than other skin sites. The composition of the present invention is characterized by having an excellent effect on the lip skin as described above. In one embodiment of the present invention, it was confirmed that the magnolia bark extract has an excellent wrinkle improvement effect on both the canthus and the lip, and it was confirmed that there is a higher improvement rate and improvement rate on the lip compared to the canthus.

The lip skin cosmetic composition may be manufactured in a dosage form selected from the group consisting of lipstick, lip gloss, and lip balm, but is not limited thereto.

In the present invention, the cosmetic material composition may be manufactured in a dosage form selected from the group consisting of a solution, a topical ointment, a skin cream, a foam, a nutritional lotion, a softening lotion, a mask, a softening water, an emulsion, a foundation, a essence, a soap, a liquid cleansing material, a bathing agent, a sunscreen cream, a suntan oil, a suspension, an emulsion, a paste, a gel, a skin lotion, a powder, a soap, a surfactant-containing face wash, a skin care oil, a powder foundation, an emulsion foundation, a wax foundation, a patch, and a spray, but is not limited thereto.

The cosmetic composition of the present invention may further contain one or more cosmetically acceptable carriers to be blended with general skin cosmetics, and may be appropriately mixed with, for example, an oil component, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a pigment, a preservative, a perfume, and the like as usual ingredients, but is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic material composition of the present invention varies depending on the dosage form.

In the case where the formulation of the present invention is an ointment, paste, skin cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide or a mixture thereof may be used as a carrier component.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier ingredient, and particularly, in the case of a spray, a propellant such as chlorofluorocarbon, propane/butane or dimethyl ether may be further contained.

In the case where the dosage form of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier may be used as a carrier component, and for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol oil, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol fatty acid ester, polyethylene glycol or fatty acid ester of sorbitol may be used.

In the case where the dosage form of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol; suspending agents such as ethoxylated isostearyl alcohols, polyoxyethylene sorbitol esters, and polyoxyethylene sorbitol esters; microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar or tragacanth, etc. as carrier components.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid half-ester salt, a fatty acid protein hydrolysate, an isethionate salt, a lanolin derivative, a fatty alcohol, a vegetable oil, glycerin, a sugar, or the like can be used as a carrier component.

The cosmetic composition of the present invention may comprise, in addition to one or more substances selected from the group consisting of formononetin, emodin, atopine C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetylmandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, Raspberry ketone (Raspberry ketone), gallic acid (gallic acid), terpineol (terpineol), sodium mannose phosphate (sodium mannose phosphate), tropolone (tropolone), glycyrrhetinic acid (glycyrrhetic acid), lanosterol (lanosterol), sodium riboflavin phosphate (sodium riboflavacin phosphate), menadione (menadione), lipoic acid (thioctic acid), lactoflavin (lactoflavin), onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and fractions of the extracts, commonly used adjuvants, for example, hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic activators, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, absorbents, dyes, and the like.

In another aspect of the present invention there is provided a pharmaceutical composition comprising a compound selected from the group consisting of formononetin, emodin, atopium C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, Raspberry ketones (Raspberry ketone), gallic acid (gallic acid), terpineol (terpineol), a food composition for increasing skin elasticity and improving wrinkles, comprising as an active ingredient one or more selected from the group consisting of sodium mannose phosphate (sodium mannose phosphate), tropolone (tropolone), glycyrrhetinic acid (glycyrrhetic acid), lanosterol (lanosterol), sodium riboflavin phosphate (sodium riboflavin phosphate), menadione (menadione), lipoic acid (thioctic acid), lactoflavin (lactoflavin), onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and fractions of said extracts. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting differentiation of human adipose-derived stem cells into adipocytes, or promoting proliferation of human adipose-derived stem cells.

The formononetin, emodin, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, Raspberry ketone (Raspberry ketone), gallic acid (gallic acid), terpineol (terpineol), sodium mannose phosphate (sodium mannose phosphate), tropolone (tropolone), glycyrrhetinic acid (glycyrrhetic acid), lanosterol (lanosterol), sodium riboflavin phosphate (sodium riboflavin phosphate), menadione (menadione), lipoic acid (thioctic acid), lactoflavin (lactoflavin), onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and fractions of the extracts increase and improve the elasticity of skin as described above.

In addition, the present invention provides a food composition for increasing skin elasticity and improving wrinkles, comprising a bromatoin, emodin, atopirine C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, raspberry ketone, gallic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin sodium phosphate, menadione, lipoic acid, lactoflavin, or xanthophyllin-based dietetically acceptable salts as an active ingredient.

In the present invention, the "dietetically allowable salt" is in the form of a salt of formononetin, emodin, atopirine C, carnosic acid, palmamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, raspberry ketone, gallic acid, terpineol, sodium mannosephosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin phosphate sodium, menadione, lipoic acid, lactoflavin, or xanthophyll in combination with other substances, and means a substance capable of exhibiting an activity similar to that of the compound in dietetics.

In addition to a compound selected from the group consisting of formononetin, emodin, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acids, xanthophylls, Raspberry ketones (Raspberry ketone), gallic acid (gallic acid), terpineol (terpineol), the food composition of the present invention may contain, in addition to one or more of the group consisting of sodium mannose phosphate (sodium mannose phosphate), tropolone (tropilone), glycyrrhetinic acid (glycyrrhetic acid), lanosterol (lanosterol), sodium riboflavin phosphate (sodium riboflavin phosphate), menadione (menadione), lipoic acid (thioctic acid), lactoflavin (lactoflavin), onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and fractions of said extracts, food auxiliary additives that are acceptable in terms of food science.

In the present invention, the "food auxiliary additive" refers to a component that can be added to a food in an auxiliary manner, and is added at the time of production of a health functional food of various types, and can be used by those skilled in the art by appropriate selection. Examples of the food auxiliary additives include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages, and the like, but are not limited thereto.

The functional food composition of the present invention may include health functional foods. In the present invention, the "health functional food" refers to a food which is produced and processed in the form of tablets, capsules, powders, granules, liquids, pills, and the like, using raw materials or ingredients having functionality useful for the human body. Here, "functional" means that it has a beneficial effect on the structure and function of the human body to regulate nutrients or on health care purposes such as physiological action. The health functional food of the present invention can be manufactured by a method commonly used in the art, and can be manufactured by adding raw materials and ingredients commonly added in the art at the time of manufacturing. The formulation of the health functional food can be produced without limitation as long as it is considered to be a formulation of the health functional food. The food composition of the present invention can be manufactured in various forms of dosage forms, and unlike general medicines, it has advantages of no side effects due to long-term administration, etc. by using food as a raw material, and is excellent in portability, so that the health functional food of the present invention can be ingested as an adjuvant for increasing skin elasticity and improving wrinkles.

The health functional food of the present invention may take a form without limitation, and may include all foods in a conventional meaning, or may be used in combination with terms known in the art, such as functional foods. Also, the health functional food of the present invention can be manufactured by mixing other auxiliary components and well-known additives, which may be suitably contained in the food, according to the selection of those skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, cookies, pizza, stretched noodles, other noodles, chewing gum, dairy products such as ice cream, various soups, beverages, tea, oral liquids, alcoholic beverages, and vitamin compounds. In addition, food products for use as animal feed are also included.

In another aspect of the present invention there is provided a pharmaceutical composition comprising a compound selected from the group consisting of formononetin, emodin, atopium C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, Raspberry ketones (Raspberry ketone), gallic acid (gallic acid), terpineol (terpineol), a pharmaceutical composition for increasing skin elasticity and improving wrinkles, comprising as an active ingredient one or more selected from the group consisting of sodium mannose phosphate (sodium mannose phosphate), tropolone (tropilone), glycyrrhetinic acid (glycyrrhetic acid), lanosterol (lanosterol), sodium riboflavin phosphate (sodium riboflavin phosphate), menadione (menadione), lipoic acid (thioctic acid), lactoflavin (lactoflavin), onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and fractions of said extracts. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting differentiation of human adipose-derived stem cells into adipocytes. The formononetin, emodin, atopirin C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, xanthophylls, magnolia bark, aloe, extracts, distillates and the like increase and improve the elasticity of skin as described above.

In addition, the present invention provides a pharmaceutical composition for increasing skin elasticity and improving wrinkles, comprising pharmaceutically acceptable salts of formononetin, emodin, atopine C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid or xanthophylls as active ingredients.

In the present invention, the "pharmaceutically acceptable salt" is in the form of a salt of formononetin, emodin, atopirine C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, raspberry ketone, gallic acid, terpineol, sodium mannosyl phosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin phosphate sodium, menadione, lipoic acid, lactoflavin, or xanthophyll in combination with other substances, and means a substance capable of exhibiting pharmaceutically similar activity to the compound.

The pharmaceutical composition of the present invention may be used as a single preparation, or may be prepared as a composite preparation further comprising a known drug having effects of increasing skin elasticity and improving wrinkles, and may be prepared using a pharmaceutically acceptable carrier or excipient to be manufactured in a unit volume form, or may be manufactured by being put into a multi-volume container.

The pharmaceutical composition may be administered in a pharmaceutically effective dose. The "pharmaceutically effective dose" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment, and an effective dose level may be determined depending on factors including individual type and severity, age, sex, activity of a drug, sensitivity to a drug, administration time, administration route and excretion ratio, treatment period, concurrent use of a drug, and other factors well known in the medical field.

Another aspect of the present invention provides a pharmaceutical external composition for increasing skin elasticity and improving wrinkles, comprising, as an active ingredient, one or more selected from the group consisting of formononetin, emodin, atopirine C, carnosic acid, palmamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acids, xanthophylls, raspberry ketone, gallic acid, terpineol, sodium mannosylphosphate, tropone, glycyrrhetinic acid, lanosterol, riboflavin phosphate sodium, menadione, lipoic acid, lactoflavin, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, and fractions of the extracts. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting differentiation of human adipose-derived stem cells into adipocytes. The formononetin, emodin, atopirin C, carnosic acid, palmitamide, guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acids, xanthophylls, raspberry ketone, gallic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin phosphate sodium, menadione, lipoic acid, lactoflavin, onion, rice bran, bamboo, carrot, hibiscus, magnolia bark, aloe, extracts, fractions increase and improve skin elasticity as described above.

In addition, the present invention provides a pharmaceutical external composition for increasing skin elasticity and improving wrinkles, comprising pharmaceutically acceptable salts of formononetin, emodin, atopirine C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acid, raspberry ketone, gallic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin sodium phosphate, menadione, lipoic acid, lactoflavin, or xanthophyllin as an active ingredient. The "pharmaceutically acceptable salts" are as described above.

The pharmaceutical composition for external use of the present invention may further contain, in addition to the above components, a pharmaceutically acceptable carrier, excipient or diluent as necessary. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as the effects of the present invention are not impaired, and for example, may include fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives and the like.

The "pharmaceutically acceptable carrier" may refer to a carrier, excipient or diluent which does not irritate an organism and does not hinder the biological activity and properties of a compound to be injected, and specifically, may be a non-natural carrier (non-natural encapsulation carrier). The type of the carrier that can be used in the present invention is not particularly limited, and any pharmaceutically acceptable carrier commonly used in the art may be used. As non-limiting examples of the carrier, physiological saline, sterile water, ringer's solution, buffered physiological saline, albumin injection, dextrose solution, maltose solution, glycerol, ethanol, and the like can be given. These carriers may be used alone, or two or more thereof may be used in combination.

The composition comprising the pharmaceutically acceptable carrier may be in various dosage forms for oral or non-oral administration. When the preparation is carried out, the preparation is carried out by using a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant, which is generally used. Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like, and these solid preparations can be prepared by mixing the compound with at least one or more excipients, for example, starch, calcium carbonate, sucrose, lactose, gelatin and the like. Additionally, in addition to simple excipients, lubricants such as magnesium stearate and talc may be used. Liquid preparations for oral administration are suspensions, solutions for internal use, emulsions, syrups and the like, and may include various excipients such as wetting agents, sweeteners, aromatics, preservatives and the like, in addition to water and liquid paraffin, which are generally used simple diluents. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations, and suppositories. Non-aqueous solvents, suspending agents, and vegetable oils such as propylene glycol, polyethylene glycol, olive oil, and injectable esters such as ethyl oleate can be used. As the base of the suppository, WITEPSOL, polyethylene glycol (Macrogol), Tween 61, cacao butter, laurate, glycerogelatin, etc. can be used.

Examples of the pharmaceutical composition for external use of the present invention include, but are not limited to, a disinfectant detergent, a bath foam, a cream liquid, a wet wipe, a coating agent, and the like, and the method of preparing the pharmaceutical composition for external use, the dose, the method of using the composition, the components, and the like can be appropriately selected from conventional techniques known in the art.

In addition, the pharmaceutical external composition of the present invention may be used in a method for improving skin wrinkles, which comprises the step of applying the pharmaceutical external composition of the present invention to the skin of an individual. Including but not limited to mammals including mice, livestock, humans, and the like.

Still another aspect of the present invention provides a composition for promoting differentiation of human fat-derived stem cells, comprising, as an active ingredient, one or more substances selected from the group consisting of formononetin, emodin, atopirine C, carnosic acid, palmitamide, sodium guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acids, xanthophylls, raspberry ketone, gallic acid, terpineol, sodium mannosylphosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin sodium phosphate, menadione, lipoic acid, lactoflavin, onion extract, rice bran extract, bamboo extract, carrot extract, hibiscus extract, magnolia bark extract, aloe extract, a fraction of magnolia bark extract, and a fraction of aloe extract. In the present invention, the composition can increase skin elasticity and improve wrinkles by promoting differentiation of human adipose-derived stem cells into adipocytes. The formononetin, emodin, atopirin C, carnosic acid, palmitamide, guaiazulene sulfonate, caffeic acid, acetyl mandelic acid, laccaic acid, ubiquinone, hydroxycinnamic acids, xanthophylls, raspberry ketone, gallic acid, terpineol, sodium mannose phosphate, tropolone, glycyrrhetinic acid, lanosterol, riboflavin phosphate sodium, menadione, lipoic acid, lactoflavin, onion, rice bran, bamboo, carrot, hibiscus, magnolia bark, aloe, extracts, fractions increase and improve skin elasticity as described above.

Yet another aspect of the present invention provides a method of increasing skin elasticity comprising the step of administering the composition to an individual other than a human.

The term "administering" as used herein means administering the composition of the present invention to a patient by any suitable method, and the route of administration of the composition may be by any general route that is capable of reaching the target tissue. It may be administered intraperitoneally, intravenously, intramuscularly, subcutaneously, intradermally, orally, topically, intranasally, but is not limited thereto. Since the composition of the present invention is characterized by having the effects of increasing skin elasticity and improving wrinkles, the composition may be administered by applying it to the skin.

The composition of the present invention may be administered daily or intermittently, or may be administered in divided doses of 1 or 2 to 3 times per day. In addition, the composition of the present invention can be used alone or in combination with other drug treatments in order to increase skin elasticity and improve wrinkles. In view of all the above, it is important to be able to administer the drug in an amount that achieves the maximum effect at the minimum amount without side effects, and the amount can be easily determined by those skilled in the art.

The term "individual" of the present invention means all animals including mice, livestock and the like, including humans, in which reduction in skin elasticity or generation of skin wrinkles may occur. Specifically, it may be a mammal including a human.

[ embodiment ]

Hereinafter, the present invention will be described in detail by the following examples. However, the following examples are merely illustrative of the present invention, and the scope of the present invention is not limited by the following examples.

Example 1: preparation method of cortex Magnolia officinalis extract and Aloe extract

The magnolia bark extract of this example was purchased from bleekco corporation, republic of kyonggi province, korea, and the powder of magnolia bark (magnolia Cortex) was dissolved in a solvent such as dipropylene glycol (DPG), dimethyl sulfoxide (DMSO), etc., and then filtered using a 0.2 μm syringe filter, and the filtered liquid was used as the magnolia bark extract.

To manufacture the Aloe extract of this example, Aloe (Aloe Vera) was purchased from a korean domestic farm and extracted once at 70 ℃ using 70% methanol at a ratio of 1: 10.

Example 2: effect of formononetin, acetyl mandelic acid and Magnolia bark extract on promoting differentiation of human fat-derived stem cells was confirmed

Human adipose-derived stem cells (Human adipose derived stem cells) were purchased from Dragon Sand Inc (Lonza Inc, Walkersvile, MD, USA) and cultured according to the instructions of Dragon Sand Inc. Experiments were performed on whether to differentiate into adipocytes using the differentiation medium recommended by the dragon sand company. As a positive control group, the differentiation medium was treated with Rosiglitazone (Rosiglitazone)1 uM. In order to induce differentiation of adipose-derived stem cells into adipocytes, after 3 to 5 days from the start of culturing the cells, the cell culture solution was removed, and the positive control group was treated with formononetin, acetyl mandelic acid, and Magnolia bark extract for 14 days.

After 14 days from the start of differentiation into adipocytes, the cell culture solution was removed, the cells were washed with phosphate buffered saline (DPBS), and then the cells were fixed with 10% formalin. The lipids within the differentiated adipocytes were stained with oil Red o (oil Red o) to observe the fixed cells with a microscope. As a result, it was found that the differentiation effect of the adipose-derived stem cells into adipocytes was superior to that of the differentiation medium alone (negative control group) when the stem cells were treated with Formononetin (Formononetin), Acetyl Mandelic acid (Acetyl maleic acid), or magnolia bark extract, which are typical substances (fig. 1).

Example 3: quantitative confirmation of the Effect of 12 compounds including formononetin, Magnolia bark extract and Aloe extract on promoting differentiation of human fat-derived stem cells

In the same manner as in example 2 above, after cultured human fat-derived stem cells were treated with 12 compounds including formononetin, magnolia bark extract and aloe extract, oil red O for staining was eluted with 100% isopropyl alcohol solution, and then absorbance was measured at a wavelength of 490nm and quantified, the results of which are shown in fig. 2. It was thus confirmed that 14 substances including formononetin according to the present invention each showed a significant increase in relative differentiation rate compared to the negative control group treated with only differentiation medium, thereby promoting differentiation of adipose-derived stem cells into adipocytes, and effectively promoting differentiation into adipocytes compared to undifferentiated material such as kaempferol (kaempferol). Therefore, the composition of the present invention comprising the same can be used as an anti-aging purpose for increasing the elasticity of skin and improving wrinkles by promoting the differentiation of adipose-derived stem cells and increasing subcutaneous adipocytes.

Example 4: confirming the effect of Magnolia officinalis extract on improving lip skin wrinkles

In order to confirm the effect of the Magnolia bark extract produced in example 1 on the improvement of skin wrinkles, wrinkles on the lip skin and under-eye skin of the subjects (total number of subjects: 12) were treated with the Magnolia bark extract.

Wrinkles of the lip skin were measured by designating the most protruding part of the lower lip of a certain length by a Janus (Janus) photograph taking and finding the standard deviation of the light and dark values. It was confirmed that the standard deviation of the light and dark values ranged from 3 to 12, and the more wrinkles, the larger the standard deviation. The wrinkles of the skin under the eyes are measured by means of a bernoulli photograph, using the wrinkle analysis program in bernoulli, i.e. by calculating the area of the wrinkles. It was confirmed that the value of the wrinkles was in the range of 7 to 44.

Results in which the change in lip wrinkle value was 1 or more and the change in under-eye wrinkle value was 4 or more were classified as improved result values. The classification criterion was selected because a value that can clearly distinguish whether or not improvement was achieved with the naked eye, that is, a lip wrinkle was 2 and a sub-ocular wrinkle was 8, was confirmed, and 1/2 value of the value was selected as a criterion for judging whether or not improvement was achieved.

The results of lip skin treatment with magnolia bark extract confirmed that the improvement started at week 4 and the number of subjects (8) improved at week 8 reached a maximum, and confirmed that this remained similar until week 12. The result of treatment of under-eye wrinkles with the magnolia bark extract confirmed improvement at a similar rate to lip skin by week 4, and although the rate of rise was lower, the number of subjects improving until week 12 continued to increase (fig. 3).

In addition, it was confirmed that the wrinkle improvement effect was continued up to week 12 and the elasticity of the lip skin was improved after the lip skin of the subject was treated with magnolia bark extract (fig. 4). This is a result of confirming that the magnolia bark extract actually produces an effect of improving wrinkles and elasticity of the skin by promoting differentiation of adipose stem cells and increasing subcutaneous adipose cells.

As a result of the wrinkle-improving effect on the lip skin and the under-eye skin compared with asiaticoside, which is a conventional wrinkle-improving material, it was confirmed that the speed and rate of improvement of wrinkles on the lip skin by magnolia bark extract are more excellent than those on the under-eye skin, and the speed and rate of improvement of wrinkles on the under-eye skin by asiaticoside are more excellent than those on the lip skin (fig. 5).

It is seen that the magnolia bark extract has an effect of improving wrinkles of the skin, and particularly, has an excellent effect of improving wrinkles of the skin on the lips.

Example 5: preparation method of extracts of Bulbus Allii Cepae, testa oryzae, bamboo, radix Dauci Sativae and Hibisci

In the present invention, the extracts of onion, rice bran, bamboo, carrot and hibiscus are extracted with methanol, respectively, and then dried to be used in the form of powder.

Example 6: the effect of the compound and the extract on the promotion of the proliferation of human adipose-derived stem cells was confirmed

Human adipose derived stem cells (human adipose derived stem cells) were purchased from Longsha corporation (Lonza, Inc. Walkersville, MD, USA) and cultured in a carbon dioxide medium at 37 ℃ under 5% carbon dioxide. After each material was treated at concentrations of 0.2ppm, 2ppm and 20ppm, the degree of cell activity of human adipose stem cells was confirmed using CCK-8 kit (Dojindo: Hojindo chemical). As a result, each material showed the highest activity at the concentration of table 1 below, and all materials showed a higher cell activity rate of 20% or more compared to the untreated group (control group) (fig. 6).

[ Table 1]

Name of Material	Concentration of
		Xanthophylls (xanthophylls)	20ppm
Lactoflavin	20ppm
		Lipoic acid (lipoid acid)	20ppm
Menadione	0.2ppm
		Riboflavin sodium phosphate	20ppm
Lanosterol	2ppm
		Glycyrrhetinic acid	20ppm
Mannose phosphate sodium salt	2ppm
		Tropolone	2ppm
Terpineol	20ppm
		Carnosic acid	2ppm
Caffeic acid	20ppm
		Gallic acid	20ppm
Raspberry ketone	20ppm
		Onion (onion)	2ppm
Rice bran	20ppm
		Bamboo	2ppm
Carrot (Carex sativus L.)	20ppm
		Hibiscus syriacus L	20ppm

Therefore, a total of 19 compounds or extracts of the present invention significantly promoted the proliferation of human adipose-derived stem cells, and thus could be used for anti-aging purposes by increasing the elasticity of the skin and improving wrinkles.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without changing the technical spirit or essential features thereof. In this connection, the above-described embodiments are exemplary in all respects and should be understood as non-limiting. The scope of the present invention should be construed to include the meaning and scope of the claims set forth below and all changes or modifications derived from the equivalent concept thereof, rather than the detailed description set forth above.

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