阅读说明：本技术 一种不易沉降的蝌蚪粉状饲料及其制备方法和应用 (Tadpole powdery feed not prone to settling and preparation method and application thereof ) 是由 贠彪 涂永芹 于 2020-12-25 设计创作，主要内容包括：本发明涉及一种不易沉降的蝌蚪粉状饲料及其制备方法和应用,包括重量份数组分：秘鲁蒸汽鱼粉15-20份,猪肉粉10-15份,水解羽毛粉3-5份,豆粕10-15份,棉籽蛋白8-15份,花生粕10-15份,膨化米糠15-20份,膨化玉米10-15份,磷酸二氢钙1-2份,维生素和矿物盐预混物1.4-2份,氯化胆碱0.1-0.5份,抗氧化剂0.01-0.05份,防霉剂0.01-0.05份。本发明：1)漂浮时间延长；2)表面积和混合均匀度增加；3)降低粉料密度并增加物料表面积,两种方式叠加进一步增加减缓沉降效果；4)饲料利用率提高；5)养殖水体氮磷排放量降低；6)提高了蝌蚪的存活率、变态率和生长速度。(The invention relates to a tadpole powdery feed not prone to sedimentation and a preparation method and application thereof, wherein the tadpole powdery feed comprises the following components in parts by weight: 15-20 parts of Peru steam fish meal, 10-15 parts of pork powder, 3-5 parts of hydrolyzed feather meal, 10-15 parts of soybean meal, 8-15 parts of cottonseed protein, 10-15 parts of peanut meal, 15-20 parts of expanded rice bran, 10-15 parts of expanded corn, 1-2 parts of monocalcium phosphate, 1.4-2 parts of vitamin and mineral salt premix, 0.1-0.5 part of choline chloride, 0.01-0.05 part of antioxidant and 0.01-0.05 part of mildew preventive. The invention comprises the following steps: 1) the floating time is prolonged; 2) increased surface area and mixing uniformity; 3) the density of the powder is reduced, the surface area of the material is increased, and the sedimentation slowing effect is further increased by superposing the two modes; 4) the utilization rate of the feed is improved; 5) the nitrogen and phosphorus discharge amount of the aquaculture water body is reduced; 6) the survival rate, metamorphosis rate and growth speed of tadpoles are improved.)

1. The tadpole powdery feed not prone to settling is characterized by comprising the following components in parts by weight: 15-20 parts of Peru steam fish meal, 10-15 parts of pork powder, 3-5 parts of hydrolyzed feather meal, 10-15 parts of soybean meal, 8-15 parts of cottonseed protein, 10-15 parts of peanut meal, 15-20 parts of expanded rice bran, 10-15 parts of expanded corn, 1-2 parts of monocalcium phosphate, 1.4-2 parts of vitamin and mineral salt premix, 0.1-0.5 part of choline chloride, 0.01-0.05 part of antioxidant and 0.01-0.05 part of mildew preventive.

2. The tadpole powdery feed not prone to sedimentation according to claim 1, comprising the following components in parts by weight: 15 parts of Peru steam fish meal, 15 parts of pork powder, 3 parts of hydrolyzed feather meal, 12 parts of soybean meal, 8 parts of cottonseed protein, 14 parts of peanut meal, 15 parts of expanded rice bran, 15 parts of expanded corn, 1.2 parts of monocalcium phosphate, 1.4 parts of vitamin and mineral salt premix, 0.3 part of choline chloride, 0.05 part of antioxidant and 0.05 part of mildew preventive.

3. The tadpole powdery feed not prone to sedimentation according to claim 1, comprising the following components in parts by weight: the feed comprises, by weight, 20 parts of Peru steam fish meal, 10 parts of pork powder, 5 parts of hydrolyzed feather meal, 10 parts of soybean meal, 10 parts of cottonseed protein, 12 parts of peanut meal, 20 parts of expanded rice bran, 10 parts of expanded corn, 1 part of monocalcium phosphate, 1.75 parts of vitamin and mineral salt premix, 0.2 part of choline chloride, 0.03 part of antioxidant and 0.02 part of mildew preventive.

4. The tadpole powdery feed not prone to sedimentation according to claim 1, comprising the following components in parts by weight: 16 parts of Peru steam fish meal, 12 parts of pork powder, 4 parts of hydrolyzed feather meal, 15 parts of soybean meal, 15 parts of cottonseed protein, 10 parts of peanut meal, 15 parts of expanded rice bran, 15 parts of expanded corn, 1.3 parts of monocalcium phosphate, 1.5 parts of vitamin and mineral salt premix, 0.15 part of choline chloride, 0.02 part of antioxidant and 0.03 part of mildew preventive.

5. The tadpole powdery feed unlikely to settle according to any one of claims 1 to 4, wherein the Peru steam fish meal is Peru super steam fish meal; and/or the pork powder is domestic pork powder, the protein content is more than or equal to 68 percent, and the fat content is more than or equal to 12 percent; and/or the hydrolyzed feather powder is high-temperature and high-pressure hydrolyzed feather powder; and/or the raw materials with the fineness of more than 95 percent of the raw material components pass through a 40-mesh screen, and preferably the raw materials with the fineness of more than 99 percent of the raw material components in the feed pass through an 80-mesh screen.

6. A method for preparing tadpole powdery feed not prone to settle according to any one of claims 1-5, comprising:

(1) uniformly mixing the vitamin and mineral salt premix, choline chloride, an antioxidant and a mildew preventive to prepare a small material mixture;

(2) crushing Peru steam fish meal, pork powder, hydrolyzed feather meal, soybean meal, cottonseed protein, peanut meal, puffed rice bran, puffed corn and monocalcium phosphate serving as large raw materials, and sieving the crushed raw materials with a sieve of 40 meshes, wherein the crushed raw materials have the fineness of more than 95%;

(3) mixing the large raw material and the small material mixture uniformly, carrying out superfine grinding, passing the raw material with the superfine grinding fineness of more than 99% through a 80-mesh screen, and then mixing uniformly for the second time to obtain the powdery feed.

7. The method according to claim 6, wherein the small materials are mixed in a special mixer for the small materials, and the mixing time is 300 s; and/or coarsely crushing the large raw material, mixing the large raw material and the small material mixture in a double-shaft paddle type mixer for 180 s; and/or, the secondary mixing in the step (3) adopts a double-shaft paddle mixer, and the mixing time is 120 s.

8. The method of claim 6, comprising:

(1) mixing the vitamin and mineral salt premix, choline chloride, antioxidant and mildew preventive in a special small material mixer for 300s to obtain a small material mixture;

(2) crushing Peru steam fish meal, pork powder, hydrolyzed feather meal, soybean meal, cottonseed protein, peanut meal, puffed rice bran, puffed corn and monocalcium phosphate serving as large raw materials, and sieving the crushed raw materials with a sieve of 40 meshes, wherein the crushed raw materials have the fineness of more than 95%;

(3) uniformly mixing the large raw material and the small material mixture in a double-shaft paddle type mixer for 180s, carrying out superfine grinding, passing the raw material with the superfine grinding fineness of more than 99% through an 80-mesh screen, and carrying out secondary mixing by adopting the double-shaft paddle type mixer for 120s to uniformly obtain the powdery feed.

9. Tadpole powdery feed which is not easy to settle and is prepared according to the preparation method of any one of claims 6-8.

10. The application of the tadpole powdery feed not prone to settlement is characterized in that the tadpole powdery feed is the tadpole powdery feed in any one of claims 1-5 and 6, the use mode is that dry powder is directly and uniformly sprinkled on the water surface, the use amount is 3% -5% of the weight of the tadpole, and the tadpole powdery feed is fed until the tadpole is apparently fed with food.

Technical Field

The invention relates to a powdery feed, in particular to a tadpole powdery feed which is not easy to settle, and a preparation method and application thereof.

Background

Bullfrog (Rana catesbeian) belongs to Amphinaceae, Anhula, and is a large edible frog. The bullfrog is originally produced in North America, Mexico and other places, is introduced into China from Guba in 1959, and has the advantages of rich nutrition, delicious taste, high growth speed and strong environment adaptability. In 2019, the bullfrog breeding yield is close to 50 million tons, the sales yield exceeds 50 million yuan per year, and the bullfrog is an important special variety for aquaculture in China.

Tadpoles are larvae of the bullfrogs which are not abnormal, powdery compound feed is mainly fed in the artificial breeding process, but powder materials in the current market have the characteristics of high settling speed when meeting water (usually, the powder materials sink quickly after floating on the water for 2-3 s), no time for tadpoles to eat, and low effective utilization rate (only 30% -40%), so that the feed in the tadpole period is seriously wasted, and the cost is greatly increased. Meanwhile, the nitrogen and phosphorus content of the water body is obviously increased due to the unused powdery compound feed, the number of harmful bacteria is increased, the water quality is deteriorated, and the survival rate, the metamorphosis rate and the growth speed of the tadpoles are all reduced.

In conclusion, improvement in the settling properties of the existing tadpole powder is needed.

Disclosure of Invention

Aiming at the defects, the invention provides the tadpole powdery feed, which reduces waste and water pollution in the tadpole feeding process and improves the survival rate, metamorphosis rate and growth speed of the tadpoles.

The invention achieves the above purposes through the following scheme:

the first aspect provides a tadpole powdery feed not prone to sedimentation, which comprises the following components in parts by weight: 15-20 parts of Peru steam fish meal, 10-15 parts of pork powder, 3-5 parts of hydrolyzed feather meal, 10-15 parts of soybean meal, 8-15 parts of cottonseed protein, 10-15 parts of peanut meal, 15-20 parts of expanded rice bran, 10-15 parts of expanded corn, 1-2 parts of monocalcium phosphate, 1.4-2 parts of vitamin and mineral salt premix, 0.1-0.5 part of choline chloride, 0.01-0.05 part of antioxidant and 0.01-0.05 part of mildew preventive.

Preferably, the tadpole powdery feed not prone to sedimentation comprises the following components in parts by weight: 15 parts of Peru steam fish meal, 15 parts of pork powder, 3 parts of hydrolyzed feather meal, 12 parts of soybean meal, 8 parts of cottonseed protein, 14 parts of peanut meal, 15 parts of expanded rice bran, 15 parts of expanded corn, 1.2 parts of monocalcium phosphate, 1.4 parts of vitamin and mineral salt premix, 0.3 part of choline chloride, 0.05 part of antioxidant and 0.05 part of mildew preventive.

Preferably, the tadpole powdery feed not prone to sedimentation comprises the following components in parts by weight: the feed comprises, by weight, 20 parts of Peru steam fish meal, 10 parts of pork powder, 5 parts of hydrolyzed feather meal, 10 parts of soybean meal, 10 parts of cottonseed protein, 12 parts of peanut meal, 20 parts of expanded rice bran, 10 parts of expanded corn, 1 part of monocalcium phosphate, 1.75 parts of vitamin and mineral salt premix, 0.2 part of choline chloride, 0.03 part of antioxidant and 0.02 part of mildew preventive.

Preferably, the tadpole powdery feed not prone to sedimentation comprises the following components in parts by weight: 16 parts of Peru steam fish meal, 12 parts of pork powder, 4 parts of hydrolyzed feather meal, 15 parts of soybean meal, 15 parts of cottonseed protein, 10 parts of peanut meal, 15 parts of expanded rice bran, 15 parts of expanded corn, 1.3 parts of monocalcium phosphate, 1.5 parts of vitamin and mineral salt premix, 0.15 part of choline chloride, 0.02 part of antioxidant and 0.03 part of mildew preventive.

Preferably, the Peru steamed fish meal is Peru super steamed fish meal.

Preferably, the pork powder is domestic pork powder, the protein content is more than or equal to 68 percent, and the fat content is more than or equal to 12 percent.

Preferably, the hydrolyzed feather powder is high-temperature high-pressure hydrolyzed feather powder.

Preferably, the raw material with fineness of above 95% passes through a 40-mesh screen.

More preferably, the raw material with fineness of more than 99% in the feed passes through a 80-mesh screen.

On one hand, Peru super steam fish meal, pork meal and hydrolyzed feather meal which are fluffy in structure are used as animal protein sources, cottonseed protein and peanut meal which are light in volume weight are used as plant protein sources, and puffed rice bran and puffed corn which can float on the water surface for a long time are used as sugar sources; on the other hand, the coarse powder of the raw material with the fineness of more than 95 percent passes through a 40-mesh screen, which is different from the prior art that 80 percent passes through the 40-mesh screen; the superfine grinding fineness of the raw materials is more than 99 percent and the raw materials pass through an 80-mesh screen, which is different from the superfine grinding fineness of the raw materials in the prior art that the raw materials pass through an 80-mesh screen at 95 percent, so that the feed disclosed by the invention can not be easily settled.

In a second aspect, a preparation method of the tadpole powdery feed not prone to sedimentation is provided, and the preparation method comprises the following steps:

(1) uniformly mixing the vitamin and mineral salt premix, choline chloride, an antioxidant and a mildew preventive to prepare a small material mixture;

(2) crushing Peru steam fish meal, pork powder, hydrolyzed feather meal, soybean meal, cottonseed protein, peanut meal, puffed rice bran, puffed corn and monocalcium phosphate serving as large raw materials, and sieving the crushed raw materials with a sieve of 40 meshes, wherein the crushed raw materials have the fineness of more than 95%;

(3) mixing the large raw material and the small material mixture uniformly, carrying out superfine grinding, passing the raw material with the superfine grinding fineness of more than 99% through a 80-mesh screen, and then mixing uniformly for the second time to obtain the powdery feed.

Preferably, the small materials are mixed in a special small material mixer, and the mixing time is 300 s.

Preferably, the large raw material is coarsely pulverized and then mixed with the small raw material mixture in a double-shaft paddle mixer for 180 seconds.

Preferably, the secondary mixing in step (3) is performed by using a double-shaft paddle mixer for 120 s.

In a preferred embodiment, a preparation method of the tadpole powdery feed not prone to sedimentation comprises the following steps:

(1) mixing the vitamin and mineral salt premix, choline chloride, antioxidant and mildew preventive in a special small material mixer for 300s to obtain a small material mixture;

(2) crushing Peru steam fish meal, pork powder, hydrolyzed feather meal, soybean meal, cottonseed protein, peanut meal, puffed rice bran, puffed corn and monocalcium phosphate serving as large raw materials, and sieving the crushed raw materials with a sieve of 40 meshes, wherein the crushed raw materials have the fineness of more than 95%;

(3) uniformly mixing the large raw material and the small material mixture in a double-shaft paddle type mixer for 180s, carrying out superfine grinding, passing the raw material with the superfine grinding fineness of more than 99% through an 80-mesh screen, and carrying out secondary mixing by adopting the double-shaft paddle type mixer for 120s to uniformly obtain the powdery feed.

In a third aspect, the invention provides the tadpole powdery feed which is not easy to settle and is prepared by the preparation method.

In a fourth aspect, the invention also provides application of the tadpole powdery feed, wherein the use mode is that the dry powder is directly and uniformly sprinkled on the water surface, the use amount of the dry powder is 3% -5% of the weight of the tadpole, and the tadpole powdery feed is fed until the tadpole is apparently fed with satiety.

The application is used for improving the feed utilization rate, and/or reducing the nitrogen and phosphorus discharge amount of culture water, and/or improving the tadpole survival rate, and/or improving the tadpole metamorphosis rate, and/or improving the tadpole growth speed.

Compared with the prior art, the invention has the beneficial effects that:

1) according to the invention, animal and plant protein sources with fluffy texture or light volume weight, puffed rice bran and puffed corn which can float water are used, so that the density of the powder is effectively reduced, and the floating time of the powder on the water surface is greatly prolonged;

2) the fineness of coarse powder and superfine grinding is greatly improved, the surface area and the mixing uniformity of materials are increased, and the digestion and absorption of tadpoles are facilitated;

3) the density of the powder is reduced by selecting raw materials, the surface area of the material is increased by increasing the fineness of coarse powder and superfine powder, and the two modes are mutually superposed to further increase the effect of slowing down sedimentation;

4) the feed waste is effectively reduced, the feed utilization rate is improved, and the breeding cost is saved;

5) the nitrogen and phosphorus discharge amount of the culture water body is effectively reduced;

6) the survival rate, metamorphosis rate and growth speed of tadpoles are improved.

Detailed Description

The present invention is further illustrated by the following specific examples.

In an embodiment of the invention, the antioxidant is ethoxyquinoline.

In an embodiment of the invention, the mould inhibitor is propionic acid and its calcium salt.

In the examples of the invention, the vitamin and mineral salt premix contains the following ingredients per kg: 3.2g of vitamin A; vitamin D3, 0.5 g; vitamin E, 24 g; vitamin K3, 1 g; vitamin B1, 2.5 g; riboflavin (80%), 4.5 g; pyridoxine, 2 g; vitamin B12 (1%), 1 g; vitamin C (35%), 200 g; calcium pantothenate, 6 g; nicotinic acid, 20 g; inositol, 80 g; biotin (2%), 6 g; folic acid, 2 g; choline chloride (50%), 250 g; FeSO₄·H₂O，8g；ZnSO₄·H₂O，5g；CuSO₄·5H₂O，1g；MnSO₄·H₂O，4.5g；KI，6g；CoCl₂·6H₂O(1％)，5g；Na₂SeO₃(1％)，2g；MgSO₄·7H₂O, 120 g; NaCl, 100 g; 100g of calcium propionate and 45.8g of cellulose.

Example 1

The tadpole powdery feed not prone to settling comprises the following components in parts by weight: 15 parts of fish meal, 15 parts of pork powder, 3 parts of hydrolyzed feather meal, 12 parts of soybean meal, 8 parts of cottonseed protein, 14 parts of peanut meal, 15 parts of expanded rice bran, 15 parts of expanded corn, 1.2 parts of monocalcium phosphate, 1.4 parts of vitamin and mineral salt premix, 0.3 part of choline chloride, 0.05 part of antioxidant and 0.05 part of mildew preventive.

The preparation method comprises the following steps: 1) mixing the vitamin and mineral salt premix, choline chloride, antioxidant and mildew preventive in a special small-material mixer for 300 s; 2) respectively pulverizing fish meal, pork powder, hydrolyzed feather powder, soybean meal, cottonseed protein, peanut meal, puffed rice bran, puffed corn and calcium dihydrogen phosphate into coarse powder, and sieving with 40 mesh sieve to obtain raw materials with fineness of more than 95%; 3) mixing the coarse powder of fish meal, pork powder, hydrolyzed feather meal, bean pulp, cottonseed protein, peanut pulp, puffed rice bran, puffed corn, calcium dihydrogen phosphate and the mixture of the small materials for 180 s; 4) micronizing the mixed raw materials, and sieving the superfine crushed raw materials with fineness of more than 99% with 80 mesh sieve; 5) mixing the superfine pulverized raw materials for the second time to prevent layering, wherein the mixing time is 120s, and 6) uniformly mixing and packaging.

The tadpole powder material of the embodiment has the crude protein content of 43.1 percent and the crude fat content of 5.5 percent, and the available energy value is 18.9 kJ/g.

Example 2

The tadpole powdery feed not prone to settling comprises the following components in parts by weight: 20 parts of fish meal, 10 parts of pork powder, 5 parts of hydrolyzed feather meal, 10 parts of soybean meal, 10 parts of cottonseed protein, 12 parts of peanut meal, 20 parts of expanded rice bran, 10 parts of expanded corn, 1 part of monocalcium phosphate, 1.75 parts of vitamin and mineral salt premix, 0.2 part of choline chloride, 0.03 part of antioxidant and 0.02 part of mildew preventive.

The preparation method is the same as that of example 1.

The tadpole powder material of the embodiment has the crude protein content of 42.6 percent and the crude fat content of 5.8 percent, and the available energy value is 19.2 kJ/g.

Example 3

The tadpole powdery feed not prone to settling comprises the following components in parts by weight: 16 parts of fish meal, 12 parts of pork powder, 4 parts of hydrolyzed feather meal, 15 parts of soybean meal, 15 parts of cottonseed protein, 10 parts of peanut meal, 15 parts of expanded rice bran, 15 parts of expanded corn, 1.3 parts of monocalcium phosphate, 1.5 parts of vitamin and mineral salt premix, 0.15 part of choline chloride, 0.02 part of antioxidant and 0.03 part of mildew preventive.

The preparation method is the same as that of example 1.

The tadpole powder material of the embodiment has the crude protein content of 43.9 percent and the crude fat content of 5.3 percent, and the available energy value is 18.7 kJ/g.

Comparative example 1

The crude protein content of tadpole powder of company A which is a good product in the public praise and purchased in the market is 43.0 percent, the crude fat content is 5.5 percent, and the available energy value is 19.0 kJ/g.

Comparative example 2

The tadpole powder is the tadpole powder of company B with better product public praise purchased in the market. The crude protein content was determined to be 42.2%, the crude fat content was determined to be 5.6%, and the available energy value was 18.8 kJ/g.

Comparative Effect test example 1

The breeding test is carried out in 1 month 4 in 2019 and 29 months 4 in 2019 in Honghen village of salt in Chenghai district of Shantou city, Guangdong province. Selecting 2.7 thousands of tadpoles with healthy appearance and uniform specification, respectively transferring the tadpoles into 9 test net cages (2m multiplied by 1m, the water depth: 0.8m), randomly dividing 3000 tadpoles of each net cage into 3 groups, and feeding the powder of the comparative example 1, the comparative example 2 and the powder of the example 1 by 3 groups, wherein the powder is manually fed at the fixed time of 7:00 and 17:00 every day, so that the tadpoles are apparently saturated. Daily food intake and mortality were recorded per cage. The test period is 28 days, the tadpoles in each net cage are weighed and counted in the whole cage at the end of the test, the number of metamorphosis tadpoles is distinguished, and the test results are shown in table 1.

Table 1:

	comparative example 1	Comparative example 2	Example 1
				Initial average amount (g)	1.4±0.1	1.4±0.0	1.5±0.1
Final average dose (g)	4.5±0.3a	4.8±0.2a	6.3±0.3b
				Weight gain (%)	221.4±14.7a	242.9±10.5a	320.0±15.2b
Feeding amount (g)	4.7±0.1a	4.9±0.1a	4.1±0.1b
				Length of floating in feed water(s)	2.1±0.3a	2.0±0.2a	21.0±0.3b
Feed coefficient (weight of tadpole death during calculation)	1.52±0.09a	1.44±0.06a	0.85±0.04b
				Survival rate (%)	74.5±2.1a	78.3±3.7a	97.6±2.0b
Metamorphosis rate (number of metamorphosis/number of survival,%)	35.2±4.6a	37.8±3.1a	86.5±1.7b

Note: the difference is significant for different lower case letters after the same row data in the table (P <0.05)

As can be seen from the test data in table 1, the tadpole flour of example 1 has a significantly longer floating time in water than that of comparative example 1 and comparative example 2, the tadpole terminal body weight, weight gain rate, survival rate and metamorphosis rate of example 1 are also significantly higher than those of comparative example 1 and comparative example 2, and the feeding amount and feed coefficient are significantly lower than those of comparative example 1 and comparative example 2.

The results of the comparative test show that the powder material can obviously improve the survival rate, metamorphosis rate and growth speed of tadpoles, and simultaneously obviously reduce the feed coefficient and save the breeding cost.

Comparative Effect test example 2

The breeding test is carried out in a certain test field of salt hongzhen in Chenghai district of Shantou city of Guangdong province from 5 months 2 days in 2019 to 5 months 30 days in 2019. Selecting 1.8 thousands of tadpoles with healthy appearance and uniform specification, respectively transferring the tadpoles into 9 test net cages (2m multiplied by 1m, the water depth: 0.8m), randomly dividing 2000 tadpoles of each net cage into 3 groups, feeding the powder of the comparative example 1, the comparative example 2 and the powder of the example 2 by 3 groups, and manually feeding the tadpoles at the fixed time of 7:00 and 17:00 every day to ensure that the tadpoles are apparently full. Daily food intake and mortality were recorded per cage. The test period is 28 days, the tadpoles in each net cage are weighed and counted in the whole cage at the end of the test, the number of metamorphosis tadpoles is distinguished, and the test results are shown in table 2.

Table 2:

	comparative example 1	Comparative example 2	Example 2
				Initial average amount (g)	2.1±0.1	2.1±0.1	2.0±0.1
Final average dose (g)	6.0±0.4	5.9±0.3	8.5±0.6
				Weight gain (%)	185.7±12.4	181.0±10.6	325.0±20.9
Feeding amount (g)	6.3±0.1a	6.2±0.2a	5.6±0.1b
				Length of floating in feed water(s)	2.3±0.2a	2.6±0.3a	23.5±0.6b
Feed coefficient (weight of tadpole death during calculation)	1.61±0.11a	1.63±0.09a	0.86±0.06b
				Survival rate (%)	79.8±1.2a	82.1±1.4a	98.1±1.7b
Metamorphosis rate (number of metamorphosis/number of survival,%)	82.9±2.3a	80.7±1.5a	99.1±0.4b

Note: the difference is significant for different lower case letters after the same row data in the table (P <0.05)

As can be seen from the test data in table 2, the tadpole powdered water of example 2 floats for a significantly longer time period than those of comparative example 1 and comparative example 2, the tadpole terminal body weight, weight gain rate, survival rate and metamorphosis rate of example 2 are also significantly higher than those of comparative example 1 and comparative example 2, and the feeding amount and feed coefficient are significantly lower than those of comparative example 1 and comparative example 2.

The results of the comparative test show that the powder material can obviously improve the survival rate, metamorphosis rate and growth speed of tadpoles, and simultaneously obviously reduce the feed coefficient and save the breeding cost.

Comparative Effect test example 3

The breeding test is carried out in 5 th month and 5 th month in 2019 and 6 th month and 2 nd month in 2019 in the town of Shanshi Denghai province, Guangdong province. Selecting 1.8 thousands of tadpoles with healthy appearance and uniform specification, respectively transferring the tadpoles into 9 test net cages (2m multiplied by 1m, the water depth: 0.8m), randomly dividing 2000 tadpoles of each net cage into 3 groups, feeding the powder of the comparative example 1, the comparative example 2 and the powder of the example 3 by 3 groups, and manually feeding the tadpoles at the fixed time of 7:00 and 17:00 every day to ensure that the tadpoles are apparently full. Daily food intake and mortality were recorded per cage. The test period is 28 days, the tadpoles in each net cage are weighed and counted in the whole cage at the end of the test, the number of metamorphosis tadpoles is distinguished, and the test results are shown in table 3.

Table 3:

	comparative example 1	Comparative example 2	Example 3
				Initial average amount (g)	2.5±0.1	2.4±0.2	2.5±0.1
Final average dose (g)	6.8±0.5a	6.5±0.6a	9.7±0.5b
				Weight gain (%)	172.0±12.6a	170.8±15.8a	288.0±14.8b
Feeding amount (g)	7.2±0.2a	7.0±0.1a	6.5±0.1b
				Length of floating in feed water(s)	2.5±0.2a	2.2±0.2a	20.7±0.4b
Feed coefficient (weight of tadpole death during calculation)	1.67±0.09a	1.71±0.12a	0.90±0.05b
				Survival rate (%)	84.6±3.1a	85.2±2.7a	98.0±1.9b
Metamorphosis rate (number of metamorphosis/number of survival,%)	90.6±2.0a	91.5±2.1a	99.5±0.3b

Note: the difference is significant for different lower case letters after the same row data in the table (P <0.05)

As can be seen from the test data in table 3, the tadpole flour of example 3 floats in water for a significantly longer time period than comparative examples 1 and 2, the tadpole terminal body weight, weight gain rate, survival rate and metamorphosis rate of example 3 are also significantly higher than those of comparative examples 1 and 2, and the feeding amount and feed coefficient are significantly lower than those of comparative examples 1 and 2.

The results of the comparative test show that the powder material can obviously improve the survival rate, metamorphosis rate and growth speed of tadpoles, and simultaneously obviously reduce the feed coefficient and save the breeding cost.

Comparative Effect test example 4

In order to show that the components in the tadpole powder have a synergistic effect, 5 tadpole powders with single component loss are prepared on the basis of example 1. The No. 1 Peru super steam fish meal is deleted and replaced by pork meal; the No. 2 lacks pork powder, but replaces Peru super steam fish powder; the 3 rd method lacks hydrolyzed feather powder and is replaced by Peru super steam; the 4 th strain lacks expanded rice bran and is replaced by expanded corn; the 5 th lacks expanded corn and is replaced by expanded rice bran. The formula of the 5 tadpole powder with single component missing is shown in table 4.

The breeding test is carried out in a certain test field of Chenghai district salt hongzhen in Shantou city of Guangdong province from 3 months 2 days in 2019 to 3 months 30 days in 2019. Selecting 3.6 thousands of tadpoles with healthy appearance and uniform specification, respectively transferring the tadpoles into 18 test net cages (2m multiplied by 1m, the water depth: 0.8m), randomly dividing 2000 tadpoles of each net cage into 6 groups, feeding the powder of the embodiment 1 and the deletion examples 1-5 by 3 groups, and manually feeding the tadpoles at the timing of 7:00 and 17:00 every day to ensure that the tadpoles are apparently full. Daily food intake and mortality were recorded per cage. The test period is 28 days, the tadpoles in each net cage are weighed and counted in the whole cage at the end of the test, and the test results are shown in table 5.

Table 4:

	example 1	Deficiency example 1	Deletion example 2	Deletion example 3	Deletion example 4	Deletion example 5
							Peru super steam fish meal	15	0	30	18	15	15
Pork powder	15	30	0	15	15	15
							Hydrolyzed feather powder	3	3	3	0	3	3
Bean pulp	12	12	12	12	12	12
							Cottonseed protein	8	8	8	8	8	8
Peanut meal	14	14	14	14	14	14
							Puffed rice bran	15	15	15	15	0	30
Puffed corn	15	15	15	15	30	0
							Calcium dihydrogen phosphate	1.2	1.2	1.2	1.2	1.2	1.2
Vitamin premix	0.4	0.4	0.4	0.4	0.4	0.4
							Mineral salt premix	1	1	1	1	1	1
Choline chloride	0.3	0.3	0.3	0.3	0.3	0.3
							Antioxidant agent	0.05	0.05	0.05	0.05	0.05	0.05
Mildew preventive	0.05	0.05	0.05	0.05	0.05	0.05
							Total up to	100	100	100	100	100	100

Table 5:

as can be seen from the test data in table 5, the floating time in the tadpole powdered water in example 1 is significantly longer than that in each of the loss examples, the tadpole terminal body weight, weight gain rate and survival rate in example 1 are also significantly higher than that in each of the loss examples, and the feeding amount is significantly lower than that in each of the loss examples. Therefore, the components of the invention have synergistic effect.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and their concepts should be considered to be equivalent or modified within the technical scope of the present invention.