A cosmetic composition containing industrial folium Cannabis extract and its preparation method
阅读说明：本技术 一种含工业大麻叶提取物的化妆品组合物及其制备方法 (A cosmetic composition containing industrial folium Cannabis extract and its preparation method ) 是由 谭昕 王曙宾 李一泽 于 2019-10-12 设计创作，主要内容包括：本发明属于化妆品领域,公开了一种含工业大麻叶提取物的化妆品组合物及其制备方法。本发明含工业大麻叶提取物的化妆品组合物由植物提取物和化妆品中可接受的辅料组成,其中,所述植物提取物中包含工业大麻叶提取物,以及白蒺藜提取物、甘草提取物、芦荟提取物、麦冬提取物中的至少一种。该化妆品组合物以天然植物提取物作为活性成分,安全高效,特别是,所制得的精华露质地柔软水润,轻薄易推开,短期使用即可看到补水保湿的功效,长期使用能够有效地焕亮肌肤,提升肌肤光泽度,所制得的凝露质地轻薄水润,容易吸收,可以长效补水保湿、有效控制皮肤油脂分泌,长期使用可以给肌肤补充营养,预防细纹和皱纹的产生。(The invention belongs to the field of cosmetics, and discloses a cosmetic composition containing an industrial hemp leaf extract and a preparation method thereof. The cosmetic composition containing the industrial cannabis sativa leaf extract comprises a plant extract and an auxiliary material acceptable in cosmetics, wherein the plant extract contains the industrial cannabis sativa leaf extract and at least one of tribulus terrestris extract, liquorice extract, aloe extract and ophiopogon japonicus extract. The cosmetic composition takes the natural plant extract as an active ingredient, is safe and efficient, and particularly, the prepared essence lotion is soft and moist in texture, light and thin and easy to push away, the effects of moisturizing can be seen in short-term use, the skin can be effectively brightened in long-term use, the skin glossiness is improved, the prepared lotion is light and thin in texture, easy to absorb, capable of moisturizing for a long time, effectively controlling skin grease secretion, and capable of supplementing nutrition to the skin and preventing fine lines and wrinkles in long-term use.)
1. A cosmetic composition comprises plant extract and cosmetically acceptable adjuvants, wherein the plant extract comprises industrial folium Cannabis extract and at least one of fructus Atriplicis Sibiricae extract, Glycyrrhrizae radix extract, Aloe extract, and radix Ophiopogonis extract;
preferably, the plant extract comprises industrial hemp leaf extract, tribulus terrestris extract and licorice extract;
or, preferably, the plant extract comprises industrial hemp leaf extract, aloe extract and ophiopogon root extract;
more preferably, the plant extract comprises 1-3 parts of industrial cannabis leaf extract, 1-3 parts of tribulus terrestris extract and 1-3 parts of licorice extract;
or, more preferably, the plant extract comprises 1-2 parts of industrial cannabis leaf extract, 1-2 parts of aloe vera extract and 1-2 parts of ophiopogon japonicus extract.
2. The cosmetic composition of claim 1, wherein the cosmetically acceptable adjuvant comprises: contains one or more of humectant, whitening agent, thickener, chelating agent, antiseptic, skin feeling regulator, pH regulator, fragrance regulator and solvent.
3. The cosmetic composition of claim 2, wherein the humectant is selected from the group consisting of: one or more of rhamnose, dextran, trehalose, hyaluronic acid and its salt, panthenol, glycerol, allantoin and propylene glycol; the dosage of the humectant is 0.1-15 parts;
preferably, the moisturizer comprises: 0.1-1 part of rhamnose, 1-3 parts of trehalose, 0.1-0.3 part of sodium hyaluronate and 1-5 parts of glycerol;
or, preferably, the humectant comprises: 0.1-1 part of glucan, 1-3 parts of trehalose, 1-3 parts of panthenol and 1-5 parts of glycerol.
4. The cosmetic composition of claim 2, wherein the whitening agent is selected from the group consisting of: one or more of nicotinamide, arbutin, ascorbic acid, glabridin and skin whitening 377; the dosage of the whitening agent is 0.1-5 parts;
preferably, the whitening agent includes: 1-3 parts of nicotinamide.
5. The cosmetic composition of claim 2, wherein said thickener is selected from the group consisting of: one or the combination of more than two of carbomer, polyacrylate cross-linked polymer-6 and sodium polyacrylate grafted starch; the amount of the thickening agent is 0.1-1 part;
preferably, the thickener comprises: 0.1-0.5 part of carbomer and 60.1-0.5 part of polyacrylate cross-linked polymer;
or, preferably, the thickener comprises: 0.1-0.5 part of carbomer and 0.1-0.5 part of sodium polyacrylate grafted starch.
6. The cosmetic composition of claim 2, wherein the chelating agent is used in an amount of 0.01 to 0.05 parts; preferably, the chelating agent comprises: 0.01-0.05 part of disodium ethylene diamine tetraacetate;
the dosage of the preservative is 0.5-2 parts; preferably, the preservative comprises: 0.5-1 part of p-hydroxyacetophenone and 0.1-0.5 part of benzoic acid.
7. The cosmetic composition of claim 2, wherein the skin feel modifier is present in an amount of 0.5 to 5 parts; preferably, the skin feel modifier comprises: 0.5-2 parts of luba gum, or the skin feeling regulator comprises: 0.5-2 parts of dendrobium polysaccharide.
8. The cosmetic composition according to claim 2, wherein the pH adjuster is used in an amount of 0.1 to 0.5 parts; preferably, the pH adjusting agent comprises: 0.1-0.5 part of triethanolamine;
the dosage of the flavor regulator is 0.01-0.05 part.
9. The cosmetic composition of claim 1, wherein the cosmetic composition comprises an a-G component, wherein:
the component A comprises: 0.1-10 parts of humectant and 10-50 parts of solvent;
the component B comprises: 0.1-1 part of thickening agent and 5-10 parts of solvent;
the component C comprises: 0.01-0.05 part of chelating agent, 0.5-2 parts of preservative and 1-5 parts of solvent;
the component D comprises: 0.5-5 parts of skin feel regulator and 1-5 parts of humectant;
the component E comprises: 0.1-0.5 part of pH value regulator and 1-5 parts of solvent;
the component F comprises: 1-10 parts of plant extract;
the G component comprises: 0.01-0.05 part of flavor regulator;
preferably, the cosmetic composition comprises a-G components, wherein:
the component A comprises: 0.1-1 part of rhamnose, 1-3 parts of trehalose, 0.1-0.3 part of sodium hyaluronate, 1-3 parts of nicotinamide and 10-50 parts of deionized water;
the component B comprises: 0.1-0.5 part of carbomer, 60.1-0.5 part of polyacrylate cross-linked polymer and 5-10 parts of deionized water;
the component C comprises: 0.01-0.05 part of disodium ethylene diamine tetraacetate, 1-5 parts of deionized water, 0.5-1 part of p-hydroxyacetophenone and 0.1-0.5 part of benzoic acid;
the component D comprises: 0.5-2 parts of luba glue and 1-5 parts of glycerol;
the component E comprises: 0.1-0.5 part of triethanolamine and 1-5 parts of deionized water;
the component F comprises: 1-3 parts of industrial hemp leaf extract, 1-3 parts of tribulus terrestris extract and 1-3 parts of liquorice extract;
the G component comprises: 0.01-0.05 part of essence;
or, the cosmetic composition includes an a-G component, wherein:
the component A comprises: 0.1-1 part of glucan, 1-3 parts of trehalose, 1-3 parts of panthenol and 10-50 parts of deionized water;
the component B comprises: 0.1-0.5 part of carbomer, 0.1-0.5 part of sodium polyacrylate grafted starch and 5-10 parts of deionized water;
the component C comprises: 0.01-0.05 part of disodium ethylene diamine tetraacetate, 1-5 parts of deionized water, 0.5-1 part of p-hydroxyacetophenone and 0.1-0.5 part of benzoic acid;
the component D comprises: 0.5-2 parts of dendrobium polysaccharide and 1-5 parts of glycerol;
the component E comprises: 0.1-0.5 part of triethanolamine and 1-5 parts of deionized water;
the component F comprises: 1-2 parts of industrial hemp leaf extract, 1-2 parts of aloe extract and 1-2 parts of radix ophiopogonis extract;
the G component comprises: 0.01-0.05 part of essence.
10. The cosmetic composition of claim 9, wherein the cosmetic composition is a serum, a gel, an emulsion, a cream, a lotion, a gel, a serum, an eye cream, a mask, an aerosol, or a spray; preferably, the cosmetic composition is a serum or a gel.
11. A process for preparing a cosmetic composition as claimed in any one of claims 1 to 10, which comprises the step of mixing the raw material components.
12. The method of claim 11, comprising the steps of:
(1) stirring and heating 50-100% of thickener and solvent in the component B to 80-85 ℃ until the thickener and the solvent are completely dissolved to form transparent liquid;
(2) adding the component A, the component C and the component D into the transparent liquid obtained in the step (1), and uniformly stirring until the components are completely dissolved to obtain a mixture 1;
(3) cooling the mixture 1 obtained in the step (2) to 40-50 ℃, adding the rest thickening agents in the component F and the component B, and dissolving and stirring uniformly to obtain a mixture 2;
(4) cooling the mixture 2 obtained in the step (3) to normal temperature, adding the component E and the component G, uniformly stirring, filtering and discharging;
the composition comprises a component A, a component B, a component D, a component F and a component G, wherein the component A comprises a humectant and a solvent, the component B comprises a thickening agent and a solvent, the component C comprises a chelating agent, a preservative and a solvent, the component D comprises a skin feel modifier and a humectant, the component E comprises a pH modifier and a solvent, the component F comprises the plant extract, and the component G comprises a fragrance modifier.
13. The method of claim 12, wherein step (1) comprises: stirring 50% of thickener and solvent in component B, and heating to 80-85 deg.C until completely dissolved to obtain transparent liquid; the step (3) comprises the following steps: cooling the mixture 1 obtained in the step (2) to 40-50 ℃, adding the rest thickening agents in the component F and the component B, and dissolving and stirring uniformly to obtain a mixture 2; or the like, or, alternatively,
the step (1) comprises the following steps: stirring the component B, and heating to 80-85 ℃ until the component B is completely dissolved to form transparent liquid; the step (3) comprises the following steps: and (3) cooling the mixture 1 obtained in the step (2) to 40-50 ℃, adding the component F, and dissolving and stirring uniformly to obtain a mixture 2.
14. Use of a plant extract in a cosmetic composition, said plant extract comprising an extract of industrial cannabis leaves and at least one of an extract of tribulus terrestris, an extract of glycyrrhiza uralensis, an extract of aloe vera, and an extract of ophiopogon japonicus;
preferably, the plant extract comprises industrial hemp leaf extract, tribulus terrestris extract and licorice extract;
or, preferably, the plant extract comprises industrial hemp leaf extract, aloe extract and ophiopogon root extract;
more preferably, the plant extract comprises 1-3 parts of industrial cannabis leaf extract, 1-3 parts of tribulus terrestris extract and 1-3 parts of licorice extract;
or, more preferably, the plant extract comprises 1-2 parts of industrial cannabis leaf extract, 1-2 parts of aloe vera extract and 1-2 parts of ophiopogon japonicus extract.
Technical Field
The invention relates to the field of cosmetics, in particular to a cosmetic composition containing an industrial hemp leaf extract and a preparation method thereof, and more particularly relates to an essence or a gel containing the industrial hemp leaf extract and a preparation method thereof.
Background
The cosmetic is a chemical industrial product or a fine chemical product which is applied to any part of the surface of a human body, such as skin, hair, nails, lips, teeth, and the like, by smearing, spraying, or the like, so as to achieve the purposes of cleaning, maintaining, beautifying, decorating, changing the appearance, or correcting the odor of the human body and maintaining a good state.
The active ingredients in the cosmetics are usually chemical compositions, the using effect is poor and unsafe, as the market of the cosmetics becomes mature, the consumer demand for the cosmetics tends to be rational, the safety of the cosmetics is emphasized when the effects such as beauty and the like are pursued, and particularly, the cosmetics which need to be used for a long time such as moisturizing, water controlling and wrinkle preventing are used.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a cosmetic composition containing industrial hemp leaf extract. The inventor of the application explores a process, and each part of industrial hemp, tribulus terrestris, liquorice and other plants is prepared into cosmetics in an extract form, and as the active ingredients of the cosmetic composition are mainly plant extracts and do not contain harmful toxic chemical components, the cosmetic composition has no potential safety hazard, and the combined plant extracts can be used for a short time to show the effects of moisturizing and nourishing the skin, and can be used for a long time to supplement nutrition for the skin.
In order to achieve the purpose of the invention, the cosmetic composition containing the industrial cannabis sativa leaf extract comprises a plant extract and cosmetically acceptable auxiliary materials, wherein the plant extract contains the industrial cannabis sativa leaf extract and at least one of tribulus terrestris extract, liquorice extract, aloe extract and radix ophiopogonis extract.
Further, the cosmetic composition may be in the form of cream, emulsion, solution, film, aerosol or spray, and more specifically, the cosmetic composition may be in the form of essence lotion, gel, lotion, cream, lotion, gel, essence, eye cream, mask, aerosol or spray. Wherein the essence can be directly applied on skin, or combined with facial mask base material (such as non-woven fabric) to make facial mask for application.
In one embodiment of the present invention, the cosmetic composition is a serum.
In another embodiment of the present invention, the cosmetic composition is a gel.
Further, in the cosmetic composition, the amount of the plant extract is 1-10 parts (by weight, the same applies hereinafter) (specifically, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 parts).
In one embodiment of the invention, the plant extract comprises industrial cannabis leaf extract, tribulus terrestris extract and licorice extract; preferably, the plant extract comprises 1-3 parts by weight of industrial hemp leaf extract, 1-3 parts by weight of tribulus terrestris extract and 1-3 parts by weight of licorice extract.
In another embodiment of the present invention, the plant extract comprises industrial cannabis leaf extract, aloe vera extract and ophiopogon japonicus extract; preferably, the plant extract comprises 1-2 parts by weight of industrial hemp leaf extract, 1-2 parts by weight of aloe extract and 1-2 parts by weight of radix ophiopogonis extract.
The above plant extracts can be prepared by conventional methods in the art, for example, by mixing the extract parts of the corresponding plants with a solvent, such as water, ethanol, etc., and extracting by conventional methods in the art, such as soaking, percolation, decoction, reflux extraction, continuous extraction, etc.
For example, in the present invention, the extraction method of the plant extract is as follows:
aloe extract: cleaning fresh aloe, cutting into small pieces, adding 2-4 times of 65-75% ethanol, cold soaking, filtering, concentrating the filtrate, recovering ethanol to obtain concentrated solution, refining with silica gel column, loading silica gel into resin column, diluting the concentrated solution with water, passing through reverse phase silica gel column, collecting all effluent, concentrating the filtrate, and drying to obtain aloe extract.
Tribulus terrestris extract: crushing the tribulus terrestris, adding 4-6 times of 15-25% ethanol for extraction, centrifuging, concentrating under reduced pressure to obtain an extract, spray-drying, and sieving with a 60-80-mesh sieve to obtain the tribulus terrestris extract.
And (3) liquorice extract: pulverizing Glycyrrhrizae radix, adding 93-97% ethanol 8-12 times of the amount of Glycyrrhrizae radix, adjusting temperature to 30-40 deg.C, ultrasonic extracting for 15-30min, filtering, concentrating under reduced pressure, and drying to obtain Glycyrrhrizae radix extract.
And (3) extracting radix ophiopogonis: taking radix Ophiopogonis dry root tuber as raw material, cleaning, pulverizing, adding 8-12 times of water, cold soaking for 20-28h, concentrating to obtain concentrated solution, adding 4-6 times of 65-75% ethanol, filtering, collecting filtrate, concentrating to obtain extract, and drying to obtain radix Ophiopogonis extract.
Industrial cannabis leaf extract: cleaning industrial hemp leaf, air drying, pulverizing, sieving, extracting with 5-7 times of 40-50% ethanol for 1-3 hr, filtering, decolorizing the filtrate, concentrating under reduced pressure, and drying to obtain industrial hemp extract.
Further, the above cosmetic field acceptable adjuvants include: contains one or more of humectant, whitening agent, thickener, chelating agent, antiseptic, skin feeling regulator, pH regulator, fragrance regulator and solvent.
Further, the above-mentioned moisturizer may be one conventional in the art, and for example, may be selected from: one or more of rhamnose, dextran, trehalose, hyaluronic acid and its salt (such as sodium hyaluronate), panthenol, glycerol, allantoin and propylene glycol.
Further, in the cosmetic composition, the humectant is used in an amount of 0.1-15 parts (specifically, 0.1, 1, 2, 4, 6, 8, 10, 12, 15 parts).
In one embodiment of the present invention, the humectant includes: 0.1-1 part of rhamnose, 1-3 parts of trehalose, 0.1-0.3 part of sodium hyaluronate and 1-5 parts of glycerol.
In another embodiment of the present invention, the humectant includes: 0.1-1 part of glucan, 1-3 parts of trehalose, 1-3 parts of panthenol and 1-5 parts of glycerol.
Further, the above whitening agent may be a whitening agent conventional in the art, for example, selected from: one or more of nicotinamide, arbutin, ascorbic acid, glabridin and skin whitening 377.
Further, in the cosmetic composition, the whitening agent is used in an amount of 0.1 to 5 parts (specifically, 0.1, 0.5, 1, 2, 3, 4, 5 parts).
In one embodiment of the present invention, the whitening agent includes: 1-3 parts of nicotinamide.
Further, the thickener may be a thickener conventional in the art, for example, a polyacrylic thickener, specifically, carbomer, polyacrylate crosspolymer-6, sodium polyacrylate grafted starch, and the like.
Further, in the cosmetic composition, the thickener is used in an amount of 0.1 to 1 part (specifically, 0.1, 0.2, 0.4, 0.5, 0.6, 0.8, 1.0 part).
In one embodiment of the present invention, the thickener includes: 0.1-0.5 part of carbomer and 60.1-0.5 part of polyacrylate cross-linked polymer.
In another embodiment of the present invention, the thickener comprises: 0.1-0.5 part of carbomer and 0.1-0.5 part of sodium polyacrylate grafted starch.
Further, the chelating agent may be a chelating agent conventional in the art, such as disodium ethylenediaminetetraacetate and the like.
Further, in the cosmetic composition, the chelating agent is used in an amount of 0.01 to 0.05 parts (specifically, 0.01, 0.02, 0.03, 0.04, 0.05 parts).
In one embodiment of the present invention, the chelating agent includes: 0.01-0.05 part of disodium ethylene diamine tetraacetate.
Further, the above-mentioned preservative may be a preservative conventionally used in the art, such as p-hydroxyacetophenone, benzoic acid, and the like.
Further, in the cosmetic composition, the amount of the preservative is 0.5 to 2 parts (specifically, 0.5, 0.6, 0.8, 1.0, 1.2, 1.4, 1.5, 1.6, 1.8, 2.0 parts).
In one embodiment of the present invention, the above-mentioned preservative comprises: 0.5-1 part of p-hydroxyacetophenone and 0.1-0.5 part of benzoic acid.
Further, the skin feeling regulator can be conventional skin feeling regulator in the art, such as jojoba gum, dendrobium polysaccharide, etc.
Further, in the cosmetic composition, the skin feel modifier is used in an amount of 0.5 to 5 parts (specifically, 0.5, 1, 1.5, 2, 2.5, 3, 4, 5 parts).
In one embodiment of the present invention, the skin-feel modifier comprises: 0.5-2 parts of luba glue.
In another embodiment of the present invention, the skin-feel modifier comprises: 0.5-2 parts of dendrobium polysaccharide.
Further, the above pH adjuster may be a pH adjuster conventional in the art, such as triethanolamine and the like.
Further, in the cosmetic composition, the amount of the pH regulator is 0.1-0.5 parts (specifically, 0.1, 0.2, 0.3, 0.4, 0.5 parts).
In one embodiment of the present invention, the pH adjuster includes: 0.1 to 0.5 portion of triethanolamine.
Further, the above-mentioned flavor modifier may be a flavor modifier conventional in the art, such as essence, etc.
Further, in the cosmetic composition, the amount of the fragrance modifier is 0.01-0.05 parts (specifically, 0.01, 0.02, 0.03, 0.04, 0.05 parts).
Further, the solvent may be a solvent conventional in the art, such as water (specifically, deionized water), and the amount of the solvent is a conventional amount in the art.
In one embodiment of the present invention, the raw materials of the above cosmetic composition comprise a-G components, wherein:
the component A comprises: 0.1-10 parts of humectant and 10-50 parts of solvent;
the component B comprises: 0.1-1 part of thickening agent and 5-10 parts of solvent;
the component C comprises: 0.01-0.05 part of chelating agent, 0.5-2 parts of preservative and 1-5 parts of solvent;
the component D comprises: 0.5-5 parts of skin feel regulator and 1-5 parts of humectant;
the component E comprises: 0.1-0.5 part of pH value regulator and 1-5 parts of solvent;
the component F comprises: 1-10 parts of plant extract;
the G component comprises: 0.01-0.05 part of flavor regulator.
In one embodiment of the present invention, the above F component includes: 1-3 parts of industrial hemp leaf extract, 1-3 parts of tribulus terrestris extract and 1-3 parts of liquorice extract.
In another embodiment of the present invention, the above F component comprises: 1-2 parts of industrial hemp leaf extract, 1-2 parts of aloe extract and 1-2 parts of radix ophiopogonis extract.
In one embodiment of the present invention, the above a component further comprises: 0.1-5 parts of whitening agent.
In one embodiment of the present invention, the raw materials of the above cosmetic composition comprise a-G components, wherein:
the component A comprises: 0.1-1 part of rhamnose, 1-3 parts of trehalose, 0.1-0.3 part of sodium hyaluronate, 1-3 parts of nicotinamide and 10-50 parts of deionized water;
the component B comprises: 0.1-0.5 part of carbomer, 60.1-0.5 part of polyacrylate cross-linked polymer and 5-10 parts of deionized water;
the component C comprises: 0.01-0.05 part of disodium ethylene diamine tetraacetate, 1-5 parts of deionized water, 0.5-1 part of p-hydroxyacetophenone and 0.1-0.5 part of benzoic acid;
the component D comprises: 0.5-2 parts of luba glue and 1-5 parts of glycerol;
the component E comprises: 0.1-0.5 part of triethanolamine and 1-5 parts of deionized water;
the component F comprises: 1-3 parts of industrial hemp leaf extract, 1-3 parts of tribulus terrestris extract and 1-3 parts of liquorice extract;
the G component comprises: 0.01-0.05 part of essence;
specifically, the cosmetic composition is essence.
In one embodiment of the present invention, the raw materials of the above cosmetic composition comprise a-G components, wherein:
the component A comprises: 0.1-1 part of glucan, 1-3 parts of trehalose, 1-3 parts of panthenol and 10-50 parts of deionized water;
the component B comprises: 0.1-0.5 part of carbomer, 0.1-0.5 part of sodium polyacrylate grafted starch and 5-10 parts of deionized water;
the component C comprises: 0.01-0.05 part of disodium ethylene diamine tetraacetate, 1-5 parts of deionized water, 0.5-1 part of p-hydroxyacetophenone and 0.1-0.5 part of benzoic acid;
the component D comprises: 0.5-2 parts of dendrobium polysaccharide and 1-5 parts of glycerol;
the component E comprises: 0.1-0.5 part of triethanolamine and 1-5 parts of deionized water;
the component F comprises: 1-2 parts of industrial hemp leaf extract, 1-2 parts of aloe extract and 1-2 parts of radix ophiopogonis extract;
the G component comprises: 0.01-0.05 part of essence;
specifically, the cosmetic composition is a gel.
The invention also provides a preparation method of the cosmetic composition, which comprises the step of mixing the raw material components.
In one embodiment of the present invention, the above preparation method comprises the steps of:
(1) stirring and heating 50-100% of thickener and solvent in the component B to 80-85 ℃ until the thickener and the solvent are completely dissolved to form transparent liquid;
(2) adding the component A, the component C and the component D into the transparent liquid obtained in the step (1), and uniformly stirring until the components are completely dissolved to obtain a mixture 1;
(3) cooling the mixture 1 obtained in the step (2) to 40-50 ℃, adding the rest thickening agents in the component F and the component B, and dissolving and stirring uniformly to obtain a mixture 2;
(4) and (4) cooling the mixture 2 obtained in the step (3) to normal temperature, adding the component E and the component G, uniformly stirring, filtering and discharging.
The composition comprises a component A, a component B, a component D, a component F and a component G, wherein the component A comprises a humectant and a solvent, the component B comprises a thickening agent and a solvent, the component C comprises a chelating agent, a preservative and a solvent, the component D comprises a skin feel modifier and a humectant, the component E comprises a pH modifier and a solvent, the component F comprises the plant extract, and the component G comprises a fragrance modifier.
In one embodiment of the present invention, step (1) comprises: stirring 50% of thickener and solvent in component B, and heating to 80-85 deg.C until completely dissolved to obtain transparent liquid; the step (3) comprises the following steps: and (3) cooling the mixture 1 obtained in the step (2) to 40-50 ℃, adding the rest thickening agents in the component F and the component B, and dissolving and stirring uniformly to obtain a mixture 2.
In another embodiment of the present invention, step (1) comprises: stirring the component B, and heating to 80-85 ℃ until the component B is completely dissolved to form transparent liquid; the step (3) comprises the following steps: and (3) cooling the mixture 1 obtained in the step (2) to 40-50 ℃, adding the component F, and dissolving and stirring uniformly to obtain a mixture 2.
In one embodiment of the present invention, the above a component includes: 0.1-1 part of rhamnose, 1-3 parts of trehalose, 0.1-0.3 part of sodium hyaluronate, 1-3 parts of nicotinamide and 10-50 parts of deionized water.
In another embodiment of the present invention, the above a component comprises: 0.1-1 part of glucan, 1-3 parts of trehalose, 1-3 parts of panthenol and 10-50 parts of deionized water.
In one embodiment of the present invention, the above B component includes: 0.1-0.5 part of carbomer, 60.1-0.5 part of polyacrylate cross-linked polymer and 5-10 parts of deionized water.
In another embodiment of the present invention, the above B component includes: 0.1-0.5 part of carbomer, 0.1-0.5 part of sodium polyacrylate grafted starch and 5-10 parts of deionized water.
In one embodiment of the present invention, the above C component includes: 0.01-0.05 part of disodium ethylene diamine tetraacetate, 1-5 parts of deionized water, 0.5-1 part of p-hydroxyacetophenone and 0.1-0.5 part of benzoic acid.
In one embodiment of the present invention, the D component includes: 0.5-2 parts of luba glue and 1-5 parts of glycerol.
In another embodiment of the present invention, the above D component comprises: 0.5-2 parts of dendrobium polysaccharide and 1-5 parts of glycerol.
In one embodiment of the present invention, the above E component includes: 0.1-0.5 part of triethanolamine and 1-5 parts of deionized water.
In one embodiment of the present invention, the above F component includes: 1-3 parts of industrial hemp leaf extract, 1-3 parts of tribulus terrestris extract and 1-3 parts of liquorice extract.
In another embodiment of the present invention, the above F component comprises: 1-2 parts of industrial hemp leaf extract, 1-2 parts of aloe extract and 1-2 parts of radix ophiopogonis extract.
In one embodiment of the present invention, the above-mentioned G component includes: 0.01-0.05 part of essence.
In one embodiment of the present invention, the above preparation method comprises the steps of:
(1) adding carbomer and deionized water in the component B into a stirring pot, stirring, and heating to 80-85 deg.C until completely dissolved to obtain transparent liquid;
(2) adding the component A, the component C and the component D, and stirring uniformly until the components are completely dissolved;
(3) cooling to 40-50 ℃, adding the component F and the polyacrylate cross-linked polymer-6 in the component B, and dissolving and stirring uniformly;
(4) cooling to normal temperature, adding the component E and the component G, stirring uniformly, filtering and discharging;
wherein the content of the first and second substances,
the component A comprises: 0.1-1 part of rhamnose, 1-3 parts of trehalose, 0.1-0.3 part of sodium hyaluronate, 1-3 parts of nicotinamide and 10-50 parts of deionized water;
the component B comprises: 0.1-0.5 part of carbomer, 60.1-0.5 part of polyacrylate cross-linked polymer and 5-10 parts of deionized water;
the component C comprises: 0.01-0.05 part of disodium ethylene diamine tetraacetate, 1-5 parts of deionized water, 0.5-1 part of p-hydroxyacetophenone and 0.1-0.5 part of benzoic acid;
the component D comprises: 0.5-2 parts of luba glue and 1-5 parts of glycerol;
the component E comprises: 0.1-0.5 part of triethanolamine and 1-5 parts of deionized water;
the component F comprises: 1-3 parts of industrial hemp leaf extract, 1-3 parts of tribulus terrestris extract and 1-3 parts of liquorice extract;
the G component comprises: 0.01-0.05 part of essence.
In another embodiment of the present invention, the above preparation method comprises the steps of:
(1) adding the component B into a stirring pot, stirring and heating to 80-85 ℃ until the component B is completely dissolved to form transparent liquid;
(2) adding the component A, the component C and the component D, and stirring uniformly until the components are completely dissolved;
(3) cooling to 40-50 deg.C, adding component F, and dissolving uniformly
(4) Cooling to normal temperature, adding the component E and the component G, stirring uniformly, filtering and discharging;
wherein the content of the first and second substances,
the component A comprises: 0.1-1 part of glucan, 1-3 parts of trehalose, 1-3 parts of panthenol and 10-50 parts of deionized water;
the component B comprises: 0.1-0.5 part of carbomer, 0.1-0.5 part of sodium polyacrylate grafted starch and 5-10 parts of deionized water;
the component C comprises: 0.01-0.05 part of disodium ethylene diamine tetraacetate, 1-5 parts of deionized water, 0.5-1 part of p-hydroxyacetophenone and 0.1-0.5 part of benzoic acid;
the component D comprises: 0.5-2 parts of dendrobium polysaccharide and 1-5 parts of glycerol;
the component E comprises: 0.1-0.5 part of triethanolamine and 1-5 parts of deionized water;
the component F comprises: 1-2 parts of industrial hemp leaf extract, 1-2 parts of aloe extract and 1-2 parts of radix ophiopogonis extract;
the G component comprises: 0.01-0.05 part of essence.
The invention also provides an application of the plant extract in a cosmetic composition, wherein the plant extract comprises an industrial cannabis leaf extract and at least one of a tribulus terrestris extract, a licorice extract, an aloe extract and an ophiopogon root extract.
In one embodiment of the invention, the plant extract comprises industrial cannabis leaf extract, tribulus terrestris extract and licorice extract; preferably, the plant extract consists of 1-3 parts by weight of industrial hemp leaf extract, 1-3 parts by weight of tribulus terrestris extract and 1-3 parts by weight of licorice extract.
In another embodiment of the present invention, the plant extract comprises industrial cannabis leaf extract, aloe vera extract and ophiopogon japonicus extract; preferably, the plant extract consists of 1-2 parts by weight of industrial hemp leaf extract, 1-2 parts by weight of aloe extract and 1-2 parts by weight of radix ophiopogonis extract.
The cosmetic composition containing the industrial hemp leaf extract, disclosed by the invention, takes the natural plant extract as an active ingredient, and is safe and efficient. Particularly, the prepared essence lotion is soft and moist, light and thin, and can be easily pushed away, the effects of moisturizing can be seen in short-term use, the skin can be effectively brightened in long-term use, the skin glossiness is improved, the prepared essence lotion is light and thin, is easy to absorb, can be used for moisturizing for a long time, effectively controls the secretion of skin grease, can be used for supplementing nutrition to the skin in a long-term use, and prevents the generation of fine lines and wrinkles.
Drawings
FIG. 1 is the average rate of change of the ITA values of the No. 7 serum sample and the No. 0 blank serum sample of the present invention;
FIG. 2 is the average rate of change of MMV values for samples No. 16 and 18 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention. It is to be understood that the following description is only illustrative of the present invention and is not to be construed as limiting the present invention.
The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.
When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.
The indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the number clearly indicates the singular. In addition, the technical features of the embodiments of the present invention may be combined with each other as long as they do not conflict with each other
In the following embodiments of the present invention, the volunteers/subjects used the cosmetic composition of the present invention did not have any adverse reactions and did not have any discomfort.
The extraction method of the plant extract in the following embodiments of the present invention is as follows:
aloe extract: cleaning fresh aloe, cutting into small pieces, adding 3 times of 70% ethanol, cold soaking, filtering, concentrating the filtrate, recovering ethanol to obtain concentrated solution, refining with silica gel column, loading silica gel into resin column, diluting the concentrated solution with water, passing through reverse phase silica gel column, collecting all effluent, concentrating the filtrate, and drying to obtain aloe extract.
Tribulus terrestris extract: crushing the tribulus terrestris, adding 5 times of 20% ethanol for extraction, centrifuging, concentrating under reduced pressure to obtain an extract, spray-drying, and sieving by a sieve of 60-80 meshes to obtain the tribulus terrestris extract.
And (3) liquorice extract: pulverizing Glycyrrhrizae radix, adding 10 times of 95% ethanol, adjusting temperature to 30-40 deg.C, ultrasonic extracting for 20min, filtering, concentrating under reduced pressure, and drying to obtain Glycyrrhrizae radix extract.
And (3) extracting radix ophiopogonis: taking radix Ophiopogonis dry root tuber as raw material, cleaning, pulverizing, adding 10 times of water, cold soaking for 24h, concentrating to obtain concentrated solution, adding 5 times of 70% ethanol, filtering, concentrating the filtrate to obtain extract, and drying to obtain radix Ophiopogonis extract.
Industrial cannabis leaf extract: cleaning industrial folium Cannabis, air drying, pulverizing, sieving, extracting with 6 times of 45% ethanol for 2 hr, filtering, decolorizing the filtrate, concentrating under reduced pressure, and drying to obtain industrial folium Cannabis extract.
Example 1
Tyrosinase activity inhibition experiment of three plant extracts of industrial hemp leaf, liquorice and tribulus terrestris: tyrosinase can catalyze a substrate tyrosine to generate dopaquinone, and then melanin is finally generated through a series of reactions, wherein the tyrosinase catalytic reaction is regarded as the rate-limiting step of melanin generation, so that the tyrosinase catalytic reaction has a substance for inhibiting tyrosinase activity and is regarded as having potential whitening activity. The dopaquinone is a colored substance, has a characteristic absorption peak under 470nm, and the generation amount of the dopaquinone is in direct proportion to the activity of the enzyme, so that the generation amount of the dopaquinone in a system can be measured by a spectrophotometer to reflect the activity of the enzyme.
The main reagents are as follows:
tyrosinase (mushroom source) (Shanghai Ech Biotech limited, 500U/mg or more); l-tyrosine (Aladdin, 99.0-101.0%); alpha-arbutin (DSM, ≧ 98%); disodium hydrogen phosphate (Na)2HPO4·12H2O), sodium dihydrogen phosphate (NaH)2PO4·2H2O) is domestic analytical purity.
Samples were prepared with the following combinations:
sample No. 1: industrial hemp leaf extract;
sample No. 2: industrial hemp leaf extract and tribulus terrestris extract, and the two extracts are mixed in equal mass;
sample No. 3: industrial hemp leaf extract and licorice extract, and mixing the two extracts in equal mass;
sample No. 4: industrial hemp leaf extract, licorice extract, tribulus terrestris extract, and the three extracts are mixed in equal mass.
The four samples were diluted 8-fold with phosphate buffer to obtain test samples.
The experimental results are as follows: and (3) carrying out test comparison on the tyrosinase inhibition rates of the four test samples, wherein the positive control is Vc. Table 1 shows the tyrosinase inhibition efficiency details of samples Nos. 1 to 4.
Details of tyrosinase inhibition rates for samples Nos. 11-4
Sample numbering
Sample No. 1
Sample No. 2
Sample No. 3
Sample No. 4
Positive control
Inhibition rate
32.35％
31.44％
30.22％
46.67％
55.44％
And (4) conclusion: in the tyrosinase activity inhibition experiment, it can be seen that sample No. 4 has a tyrosinase inhibition rate obviously higher than that of other samples and positive control samples, and the tyrosinase inhibition effect is more obvious. The industrial hemp leaf extract, the tribulus terrestris extract and the liquorice extract are combined together to have synergistic effect on the inhibition of tyrosinase activity.
Example 2
Serum DPPH free radical scavenging experiment: DPPH free radical has single electron, and has a strong absorption at 517nm, and its alcoholic solution is purple. In the presence of a free radical scavenger, its absorption gradually disappears by pairing with its single electron, and its degree of discoloration is quantitatively related to the number of electrons it receives, so that a rapid quantitative analysis can be performed with a spectrophotometer.
The main reagents are as follows: DPPH (Shanghai leaf Biotech limited, 97% or more); ascorbic acid (Vc) and methanol are all domestic analytical pure.
The essence sample is prepared according to the following raw material composition and method:
sample No. 5:
the component A comprises: 0.5 part of rhamnose, 2 parts of trehalose, 2 parts of nicotinamide, 0.2 part of sodium hyaluronate and 30 parts of deionized water;
and B component: 0.3 part of carbomer, 60.3 parts of polyacrylate cross-linked polymer and 8 parts of deionized water;
and C, component C: 0.03 part of disodium ethylene diamine tetraacetate, 3 parts of deionized water, 0.8 part of p-hydroxyacetophenone and 0.3 part of benzoic acid;
and (D) component: 1.5 parts of luba glue and 3 parts of glycerol;
and E, component (E): 0.3 part of triethanolamine and 3 parts of deionized water;
and F component: 0.5 part of industrial hemp leaf extract, 0.5 part of tribulus terrestris extract and 0.5 part of liquorice extract;
and a component G: 0.03 part of flavor regulator.
The preparation method of the essence comprises the following steps:
(1) adding carbomer and deionized water in the component B into a stirring pot, stirring, dissolving completely, heating to 80-85 deg.C, and dissolving completely to obtain transparent liquid;
(2) adding the component A, the component C and the component D, and stirring uniformly until the components are completely dissolved;
(3) cooling to 40-50 ℃, adding the polyacrylate cross-linked polymer-6 in the component F and the component B, and dissolving and stirring uniformly;
(4) cooling to normal temperature, adding the component E and the component G, stirring uniformly, filtering and discharging.
Sample No. 6 differs from sample No. 5 in that the component F in the raw material composition is: 1 part of industrial hemp leaf extract, 1 part of tribulus terrestris extract and 1 part of liquorice extract.
Sample No. 7 differs from sample No. 5 in that the component F in the raw material composition is: 2 parts of industrial hemp leaf extract, 2 parts of tribulus terrestris extract and 2 parts of liquorice extract.
Sample No. 8 differs from sample No. 5 in that the component F in the raw material composition is: 3 parts of industrial hemp leaf extract, 3 parts of tribulus terrestris extract and 3 parts of liquorice extract.
Sample No. 9 differs from sample No. 5 in that the component F in the raw material composition is: 4 parts of industrial hemp leaf extract, 4 parts of tribulus terrestris extract and 4 parts of liquorice extract.
Diluting the No. 5-9 sample with purified water by 20 times to obtain clear liquid, performing test comparison on DPPH free radical clearance of each formula, and taking a positive control as Vc. Table 2 details DPPH radical clearance.
Table 25-9 sample DPPH radical scavenging details
Sample numbering
Sample No. 5
Sample No. 6
Sample No. 7
Sample No. 8
Sample No. 9
Positive control
Clearance rate
54.47％
60.18％
63.76％
69.58％
69.85％
90.24％
And (4) conclusion: in the DPPH free radical scavenging experiment of the No. 5 to No. 9 samples, along with the increase of the content of the extract composition, the DPPH scavenging effect is better, and when the content of the No. 9 sample is increased to 4 parts of each extract, the DPPH scavenging effect is almost not different from that of the No. 8 sample, which indicates that the addition amount of the plant extract is more suitable for 1 to 3 parts of each extract.
Example 3
Skin brightness test on subjects: the volunteers applied the sample containing the essence 7 of example 2 and the blank essence containing no plant extract of sample 7 (hereinafter referred to as sample 0) to the left and right cheek sides with acne marks, respectively, and 5 subjects were tested for 6 weeks and were tested for their ITA values at 2, 4, and 6 weeks of use. The specific test results are shown in tables 3-5 below.
The average change rate of the ITA values of the sample containing the No. 7 serum sample and the No. 0 blank serum sample is shown in figure 1.
TABLE 3 subject week 2 ITA test values
TABLE 4 week 4 ITA test values for subjects
TABLE 5 subject week 6 ITA test values
As can be seen from the analysis of tables 3-5 and fig. 1, the skin-whitening effect of the essence lotion is mainly derived from the plant extracts, as a result, the skin-whitening effect of the subject using the extract lotion sample is improved to different extents, which indicates that the brightness value of the skin of the subject is improved in the process of using the essence lotion sample, and changes more significantly in the second week and the fourth week with the increase of the use time, and the effect is maintained to be constant in the process of continuing using the essence lotion sample, which indicates that the skin-whitening effect of the essence lotion can be obviously improved in the fourth week.
Example 4
A gel sample was prepared and tested for DPPH free radical scavenging as follows:
sample No. 10:
the component A comprises: 0.5 part of glucan, 2 parts of trehalose, 2 parts of panthenol and 30 parts of deionized water;
and B component: 0.3 part of carbomer, 0.3 part of sodium polyacrylate grafted starch and 8 parts of deionized water;
and C, component C: 0.03 part of disodium ethylene diamine tetraacetate, 3 parts of deionized water, 0.8 part of p-hydroxyacetophenone and 0.3 part of benzoic acid;
and (D) component: 1.5 parts of dendrobium polysaccharide and 3 parts of glycerol;
and E, component (E): 0.3 part of triethanolamine and 3 parts of deionized water;
and F component: 1.5 parts of industrial hemp leaf extract;
and a component G: 0.03 part of flavor regulator.
The preparation method of the gel comprises the following steps:
(1) adding the component B into a stirring pot, stirring and heating to 80-85 ℃ until the component B is completely dissolved to form transparent liquid;
(2) adding the component A, the component C and the component D, and stirring uniformly until the components are completely dissolved;
(3) cooling to 40-50 deg.C, adding component F, and dissolving uniformly
(4) Cooling to normal temperature, adding the component E and the component G, stirring uniformly, filtering and discharging.
Sample No. 11 differs from sample No. 10 in that the component F in the composition of the raw material is: 1.5 parts of industrial hemp leaf extract and 1.5 parts of aloe extract.
Sample No. 12 differs from sample No. 10 in that the composition of the starting material has the following F component: 1.5 parts of industrial hemp leaf extract and 1.5 parts of radix ophiopogonis extract.
Sample No. 13 differs from sample No. 10 in that the composition of the raw material has F component: 1.5 parts of industrial hemp leaf extract, 1.5 parts of aloe extract and 1.5 parts of radix ophiopogonis extract.
Respectively taking 10-13 samples to mix with DPPH (final concentration is 100 mu M) for reaction, setting 3 repeated holes, simultaneously setting Trolox positive control holes, measuring an OD value by an enzyme-linked immunosorbent assay (ELISA) instrument at 30 ℃ for 1h, wherein the detection wavelength is 515nm, and obtaining the oxidation resistance rate by calculation according to the following formula:
the oxidation resistance (%) was (1-experimental well OD 515 nm/blank well OD 515 nm). times.100%
The results are shown in Table 6.
Table 610-13 sample DPPH radical scavenging details
Sample numbering
Sample No. 10
Sample No. 11
Sample No. 12
Sample No. 13
Positive control
Clearance rate
75.25％
74.35％
80.06％
84.18％
91.44％
As can be seen from table 6, the effect of scavenging free radicals was not the best when only the industrial cannabis leaf extract was used, and varied to different extents when the cannabis leaf extract was combined with one of the aloe vera extract or the ophiopogon japonicus extract, some increased and some decreased rather than before the combination, and the effect of scavenging free radicals was the best when only the industrial cannabis leaf extract was combined with the aloe vera extract and the ophiopogon japonicus extract.
Example 5
Samples of the gel were prepared for grease secretion experiments according to the following raw material composition and the method described in example 4:
sample No. 14:
the component A comprises: 0.5 part of glucan, 2 parts of trehalose, 2 parts of panthenol and 30 parts of deionized water;
and B component: 0.3 part of carbomer, 0.3 part of sodium polyacrylate grafted starch and 8 parts of deionized water;
and C, component C: 0.03 part of disodium ethylene diamine tetraacetate, 3 parts of deionized water, 0.8 part of p-hydroxyacetophenone and 0.3 part of benzoic acid;
and (D) component: 1.5 parts of dendrobium polysaccharide and 3 parts of glycerol;
and E, component (E): 0.3 part of triethanolamine and 3 parts of deionized water;
and F component: 0.5 part of industrial hemp leaf extract, 0.5 part of aloe extract and 0.5 part of radix ophiopogonis extract;
and a component G: 0.03 part of flavor regulator.
Sample No. 15 differs from sample No. 14 in that the plant extracts are: 1 part of industrial hemp leaf extract, 1 part of aloe extract and 1 part of radix ophiopogonis extract.
Sample No. 16 differs from sample No. 14 in that the plant extracts are: 2 parts of industrial hemp leaf extract, 2 parts of aloe extract and 2 parts of radix ophiopogonis extract.
Sample No. 17 differs from sample No. 14 in that the plant extracts are: 3 parts of industrial hemp leaf extract, 3 parts of aloe extract and 3 parts of radix ophiopogonis extract.
The volunteers applied 14-17 # condensation products on the left and right sides of the forehead respectively, and the grease secretion content of the forehead after 1 hour was measured by the instrument, and the results are shown in table 7.
TABLE 7 growth rate of grease on the left and right forehead of volunteers using No. 14-17 gel products
Volunteers used sample No. 14
Left forehead
Right forehead
Initial value
106
105
Number after 1 hour
112
114
Rate of increase
5.6％
8.5％
Volunteers use sample No. 15
Left forehead
Right forehead
Initial value
103
106
Number after 1 hour
106
118
Rate of increase
2.9％
11.3％
Volunteers used sample No. 16
Left forehead
Right forehead
Initial value
113
114
Number after 1 hour
115
124
Rate of increase
1.7％
8.7％
Volunteers used sample No. 17
Left forehead
Right forehead
Initial value
101
100
Number after 1 hour
104
108
Rate of increase
2.9％
8.0％
The data in the table show that the plant extract content has obvious inhibition effect on the growth rate of the grease, when the plant extract content is increased to 3 parts, the inhibition effect is similar to that when 1 part is added, and when the addition amount is increased to 2 parts, the inhibition effect is improved, and the optimal concentration is 1-2 parts by comprehensive consideration.
Example 6
Comparative skin moisture content experiment: the gel formula (sample No. 16) containing various plant extracts is tested, and compared with the gel formula without the plant extracts (no plant extracts such as industrial hemp leaf extract, aloe extract, dwarf lilyturf tuber extract and the like are added in the sample No. 16 and are marked as a sample 18), a skin moisture content tester (Cormeter CM825) is used for detection, and the test indexes are as follows: skin moisture content MMV value.
The subjects applied the sample of gel No. 16 containing plant extract and the sample of blank gel without plant extract (i.e. sample 18) to the left and right forehead, respectively, and 5 subjects were tested for 8 weeks, and the MMV value of skin moisture content of the subjects was tested at weeks 2, 4, and 6 of the subjects. The specific test results are shown in the following tables 8-10; 16. the average rate of change of MMV values for the two samples No. 18 are shown in figure 2.
TABLE 8 moisture content at week 2 MMV value test
TABLE 9 moisture content MMV value test week 4
TABLE 10 moisture content MMV value test at week 6
As can be seen from the analysis of tables 8-10 and FIG. 2, the skin moisture content of the subject after continuous use of the sample for 6 weeks is significantly increased, the skin moisture content of the subject at the first week 2 and the second week 4 is gradually increased, and the data after 6 weeks and the data at the second week 4 are substantially equal, which indicates that the skin moisture content is good after 4 weeks of continuous use, and the skin moisture content can be continuously maintained during the continuous use.
It will be understood by those skilled in the art that the foregoing is only a preferred embodiment of the present invention, and is not intended to limit the invention, and that any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the present invention.
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