阅读说明：本技术 一种具有抑菌消炎功能的植物提取组合物及其制备方法 (Plant extract composition with antibacterial and anti-inflammatory functions and preparation method thereof ) 是由 卞亦冰 于 2020-12-24 设计创作，主要内容包括：本发明提供了一种具有抑菌消炎功能的植物提取组合物及其制备方法,该组合物由包括如下重量份的原料制成：莲子心提取物5-10份,沙葱提取物1-5份,牡丹皮提取物1-5份,枇杷叶提取物5-10份,金银花提取物1-5份。本发明所述的具有抑菌消炎功能的植物提取组合物通过将莲子心提取、沙葱提取物、牡丹皮提取物、枇杷叶提取物、金银花提取物结合,使组合物抑菌效果明显。(The invention provides a plant extract composition with antibacterial and anti-inflammatory functions and a preparation method thereof, wherein the composition is prepared from the following raw materials in parts by weight: 5-10 parts of lotus plumule extract, 1-5 parts of allium mongolicum regel extract, 1-5 parts of moutan bark extract, 5-10 parts of loquat leaf extract and 1-5 parts of honeysuckle extract. The plant extract composition with the antibacterial and anti-inflammatory functions has an obvious antibacterial effect by combining the lotus plumule extract, the allium mongolicum regel extract, the cortex moutan extract, the loquat leaf extract and the honeysuckle extract.)

1. A plant extract composition with antibacterial and anti-inflammatory functions is characterized in that: the composition is prepared from the following raw materials in parts by weight:

2. the plant extract composition with antibacterial and anti-inflammatory functions as claimed in claim 1, wherein: the composition is prepared from the following raw materials in parts by weight:

3. the plant extract composition with antibacterial and anti-inflammatory functions as claimed in claim 2, wherein: the lotus plumule extract is prepared by the following steps: oven drying plumula Nelumbinis at constant temperature, removing impurities, pulverizing, sieving to obtain plumula Nelumbinis powder, adding solvent into plumula Nelumbinis powder, adjusting pH, adding cellulase, heating in water bath, performing enzymolysis, inactivating enzyme with boiling water, filtering, centrifuging, and concentrating the supernatant.

4. The plant extract composition with antibacterial and anti-inflammatory functions as claimed in claim 3, wherein: the temperature of the drying step is 40 ℃, and the mesh opening of the sieving step is 60 meshes; the solvent is ethanol, and the feed-liquid ratio of the lotus plumule powder to the solvent is 1: 30-40; the pH is 4-5, the concentration of the cellulase is 0.1-0.2%, the temperature of the water bath heating step is 60 ℃, and the enzymolysis time is 2 h.

5. The plant extract composition with antibacterial and anti-inflammatory functions as claimed in claim 2, wherein: the allium mongolicum regel extract is prepared by the method comprising the following steps: oven drying Allium mongolicum regel at constant temperature, removing impurities, pulverizing, sieving to obtain Allium mongolicum regel powder, defatting and decolorizing to obtain Allium mongolicum regel powder, adding solvent for reflux extraction, centrifuging the extractive solution, collecting supernatant, and concentrating the supernatant; the temperature of the drying step is 60 ℃, and the mesh opening of the sieving step is 60 meshes; the solvent used in the degreasing and decoloring step is petroleum ether, the time is 10-20 hours, and the material-liquid ratio of the allium mongolicum regel powder to the petroleum ether is 1: 10-20; the solvent is ethanol, and the material-liquid ratio of the allium mongolicum regel powder to the solvent is 1: 20-30.

6. The plant extract composition with antibacterial and anti-inflammatory functions as claimed in claim 2, wherein: the moutan bark extract is prepared by the method comprising the following steps: soaking cortex moutan at 45 deg.C for 5 hr, distilling, standing, filtering, and oven drying.

7. The plant extract composition with antibacterial and anti-inflammatory functions as claimed in claim 2, wherein: the loquat leaf extract is prepared by the method comprising the following steps: drying loquat leaves at constant temperature, removing impurities, crushing and sieving to obtain loquat leaf powder, then adding ethanol for extraction, and taking supernate and concentrating to obtain the loquat leaf powder; the feed-liquid ratio of the loquat leaf powder to the ethanol is 1:20-30, and the concentration of the ethanol is 50%; the extraction temperature is 50-60 deg.C.

8. The plant extract composition with antibacterial and anti-inflammatory functions as claimed in claim 2, wherein: the honeysuckle extract is prepared by the method comprising the following steps: drying flos Lonicerae at constant temperature, removing impurities, pulverizing, sieving to obtain flos Lonicerae powder, adding ethanol for extraction, and concentrating the supernatant; the feed-liquid ratio of the loquat leaf powder to the ethanol is 1:10-20, and the concentration of the ethanol is 60%; the extraction temperature is 50-60 deg.C, and the extraction reagent is 2-4 h.

9. A method for preparing a plant extract composition with antibacterial and anti-inflammatory functions as claimed in any one of claims 1 to 9, wherein: the method comprises the following steps: mixing the lotus plumule extract, the allium mongolicum regel extract, the moutan bark extract, the loquat leaf extract and the honeysuckle extract to obtain the lotus plumule extract.

10. Use of a plant extract composition having bacteriostatic and anti-inflammatory effects as set forth in any one of claims 1 to 9, wherein: the plant extract composition with the antibacterial and anti-inflammatory functions is applied to preparation of cosmetics, toothpaste and animal feed.

Technical Field

The invention belongs to the field of bacteriostasis, and particularly relates to a plant extract composition with bacteriostasis and anti-inflammation functions and a preparation method thereof.

Background

In the prior art, most common bacteriostatic agents are chemically synthesized and biologically synthesized, have certain chronic toxic and side effects and more application limitations, and are often compounded with a plurality of preservatives to obtain broad-spectrum bacteriostatic effect only by inhibiting one or more bacteria.

In the prior art, there are also natural plant bacteriostatic preservatives which can be used in foods and daily chemical products (such as cosmetics), such as compositions with bacteriostatic efficacy consisting of plant extracts, and bacteriostatic agents consisting of plant extracts of Chinese ilex leaves, Chinese chives, mint leaves, pithecellobium clypearia, lightyellow sophora root and the like, but the addition amount of the bacteriostatic agents in foods or cosmetics is very high (the addition amount is more than 0.5 percent by mass fraction). The plant extract has higher cost than chemical or biological preservative, and the use amount is large, so the use cost is high.

Disclosure of Invention

In view of the above, the present invention provides a plant extract composition with antibacterial and anti-inflammatory functions and a preparation method thereof, aiming to overcome the defects in the prior art.

In order to achieve the purpose, the technical scheme of the invention is realized as follows:

a plant extract composition with antibacterial and anti-inflammatory functions is prepared from the following raw materials in parts by weight:

preferably, the composition is prepared from the following raw materials in parts by weight:

further, the lotus plumule extract is prepared by the method comprising the following steps: oven drying plumula Nelumbinis at constant temperature, removing impurities, pulverizing, sieving to obtain plumula Nelumbinis powder, adding solvent into plumula Nelumbinis powder, adjusting pH, adding cellulase, heating in water bath, performing enzymolysis, inactivating enzyme with boiling water, filtering, centrifuging, and concentrating the supernatant.

Further, the temperature of the drying step is 40 ℃, and the mesh size of the screening step is 60 meshes; the solvent is ethanol, and the feed-liquid ratio of the lotus plumule powder to the solvent is 1: 30-40; the pH is 4-5, the concentration of the cellulase is 0.1-0.2%, the temperature of the water bath heating step is 60 ℃, and the enzymolysis time is 2 h.

Further, the allium mongolicum regel extract is prepared by a method comprising the following steps of: oven drying Allium mongolicum regel at constant temperature, removing impurities, pulverizing, sieving to obtain Allium mongolicum regel powder, defatting and decolorizing to obtain Allium mongolicum regel powder, adding solvent for reflux extraction, centrifuging the extractive solution, collecting supernatant, and concentrating the supernatant; the temperature of the drying step is 60 ℃, and the mesh opening of the sieving step is 60 meshes; the solvent used in the degreasing and decoloring step is petroleum ether, the time is 10-20 hours, and the material-liquid ratio of the allium mongolicum regel powder to the petroleum ether is 1: 10-20; the solvent is ethanol, and the material-liquid ratio of the allium mongolicum regel powder to the solvent is 1: 20-30.

Further, the moutan bark extract is prepared by a method comprising the following steps: soaking cortex moutan at 45 deg.C for 5 hr, distilling, standing, filtering, and oven drying.

Further, the loquat leaf extract is prepared by the method comprising the following steps: drying loquat leaves at constant temperature, removing impurities, crushing and sieving to obtain loquat leaf powder, then adding ethanol for extraction, and taking supernate and concentrating to obtain the loquat leaf powder; the feed-liquid ratio of the loquat leaf powder to the ethanol is 1:20-30, and the concentration of the ethanol is 50%; the extraction temperature is 50-60 deg.C.

Further, the honeysuckle extract is prepared by the method comprising the following steps: drying flos Lonicerae at constant temperature, removing impurities, pulverizing, sieving to obtain flos Lonicerae powder, adding ethanol for extraction, and concentrating the supernatant; the feed-liquid ratio of the loquat leaf powder to the ethanol is 1:10-20, and the concentration of the ethanol is 60%; the extraction temperature is 50-60 deg.C, and the extraction reagent is 2-4 h.

The preparation method of the plant extract composition with the antibacterial and anti-inflammatory functions comprises the following steps: mixing the lotus plumule extract, the allium mongolicum regel extract, the moutan bark extract, the loquat leaf extract and the honeysuckle extract to obtain the lotus plumule extract.

The plant extract composition with the antibacterial and anti-inflammatory functions is applied to preparation of cosmetics, toothpaste and animal feed.

The lotus plumule flavonoid compounds have wide pharmacological effects, such as oxidation resistance, bacteriostasis, inflammation diminishing and the like, and have great pharmacological research values. According to the record of Mongolian medicine, allium mongolicum regel has the effects of treating red and white picking diseases and clearing away heat and toxic materials, and allium mongolicum regel volatile oil has a strong inhibiting effect on escherichia coli, staphylococcus aureus and salmonella, and the inhibiting effect is increased along with the increase of the adding amount of allium mongolicum regel volatile oil.

Allium mongolicum regel and its extract as natural plant active ingredients have strong antioxidant activity and immunoregulation activity. The allium mongolicum regel extract added into the feed can obviously improve the production performance of the broiler chicken, can obviously improve the total antioxidant capacity of the serum of the broiler chicken and the activities of glutathione peroxidase and catalase, and can obviously improve the immunity of the broiler chicken and increase the immune organ index. The flavonoids compounds can improve the contents of total superoxide dismutase, catalase and glutathione peroxidase in a test mouse body and reduce the content of malondialdehyde in the body.

Paeonol in moutan bark has the characteristics of easy volatilization, poor water solubility and easy decomposition by light, and influences the stability and bioavailability of the moutan bark in a preparation. Beta-cyclodextrin (beta-CD) is a good inclusion material, can form an inclusion compound with a medicament under certain conditions, can obviously increase the solubility of an insoluble medicament, improve the stability of the medicament, improve the bioavailability of the medicament and the like.

Loquat leaves have the functions of lowering adverse qi, arresting vomiting, clearing away lung-heat and relieving cough. The loquat leaves have strong functions of diminishing inflammation, relieving cough and eliminating phlegm; meanwhile, the inflammation diminishing and cough relieving effects of the young leaves of the loquat leaves are found to be lower than those of the old leaves of the loquat. Folium Eriobotryae contains various active components including triterpene compounds, saponins, and sesquiterpenes. The active ingredients in folium Eriobotryae have antiinflammatory, blood glucose and blood lipid reducing, and antitumor activities.

The flos Lonicerae has effects of clearing away heat and toxic materials, resisting bacteria, relieving inflammation, protecting liver, and promoting bile flow. The traditional Chinese medicine composition is clinically applied to treating diseases such as respiratory tract infection, headache and pharyngalgia. The first is antibacterial and antiviral. Namely, the compound has obvious inhibition effect on staphylococcus aureus, hemolytic streptococcus, dysentery bacillus, typhoid bacillus, meningococcus, pneumococcus, pseudomonas aeruginosa, influenza virus and the like. Secondly, the medicine has the functions of anti-inflammation and fever reduction. The honeysuckle can promote the release of adrenocortical hormone and has obvious inhibition effect on inflammation.

Compared with the prior art, the invention has the following advantages:

the plant extract composition with the antibacterial and anti-inflammatory functions has an obvious antibacterial effect by combining the lotus plumule extract, the allium mongolicum regel extract, the cortex moutan extract, the loquat leaf extract and the honeysuckle extract.

Detailed Description

Unless defined otherwise, technical terms used in the following examples have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are conventional methods unless otherwise specified.

The present invention will be described in detail with reference to examples.

The preparation method of each extract in each embodiment of the invention is as follows:

the lotus plumule extract is prepared by the following steps: oven drying plumula Nelumbinis at constant temperature, removing impurities, pulverizing, sieving to obtain plumula Nelumbinis powder, adding solvent into plumula Nelumbinis powder, adjusting pH, adding cellulase, heating in water bath, performing enzymolysis, inactivating enzyme with boiling water, filtering, centrifuging, and concentrating the supernatant; the temperature of the drying step is 40 ℃, and the mesh opening of the sieving step is 60 meshes; the solvent is ethanol, and the feed-liquid ratio of the lotus plumule powder to the solvent is 1: 35; the pH value is 5, the concentration of the cellulase is 0.2%, the temperature of the water bath heating step is 60 ℃, and the enzymolysis time is 2 hours.

The allium mongolicum regel extract is prepared by the method comprising the following steps: oven drying Allium mongolicum regel at constant temperature, removing impurities, pulverizing, sieving to obtain Allium mongolicum regel powder, defatting and decolorizing to obtain Allium mongolicum regel powder, adding solvent for reflux extraction, centrifuging the extractive solution, collecting supernatant, and concentrating the supernatant; the temperature of the drying step is 60 ℃, and the mesh opening of the sieving step is 60 meshes; the solvent used in the degreasing and decoloring step is petroleum ether, the time is 12 hours, and the feed-liquid ratio of the allium mongolicum regel powder to the petroleum ether is 1: 20; the solvent is ethanol, and the material-liquid ratio of the allium mongolicum regel powder to the solvent is 1: 20.

The moutan bark extract is prepared by the method comprising the following steps: soaking cortex moutan at 45 deg.C for 5 hr, distilling, standing, filtering, and oven drying.

The loquat leaf extract is prepared by the method comprising the following steps: drying loquat leaves at constant temperature, removing impurities, crushing and sieving to obtain loquat leaf powder, then adding ethanol for extraction, and taking supernate and concentrating to obtain the loquat leaf powder; the feed-liquid ratio of the loquat leaf powder to the ethanol is 1:20, and the concentration of the ethanol is 50%; the temperature of extraction was 60 ℃.

The honeysuckle extract is prepared by the method comprising the following steps: drying flos Lonicerae at constant temperature, removing impurities, pulverizing, sieving to obtain flos Lonicerae powder, adding ethanol for extraction, and concentrating the supernatant; the feed-liquid ratio of the loquat leaf powder to the ethanol is 1:10, and the concentration of the ethanol is 60%; the extraction temperature is 60 ℃, and the extraction reagent is used for 2 h.

Example 1

A plant extract composition with antibacterial and anti-inflammatory functions is prepared from the following raw materials in parts by weight: 5 parts of lotus plumule extract, 2 parts of allium mongolicum regel extract, 2 parts of moutan bark extract, 5 parts of loquat leaf extract and 2 parts of honeysuckle extract.

Example 2

A plant extract composition with antibacterial and anti-inflammatory functions is prepared from the following raw materials in parts by weight: 10 parts of lotus plumule extract, 2 parts of allium mongolicum regel extract, 2 parts of moutan bark extract, 5 parts of loquat leaf extract and 2 parts of honeysuckle extract.

Example 3

A plant extract composition with antibacterial and anti-inflammatory functions is prepared from the following raw materials in parts by weight: 5 parts of lotus plumule extract, 2 parts of allium mongolicum regel extract, 2 parts of moutan bark extract, 10 parts of loquat leaf extract and 2 parts of honeysuckle extract.

Example 4

A plant extract composition with antibacterial and anti-inflammatory functions is prepared from the following raw materials in parts by weight: 5 parts of lotus plumule extract, 5 parts of allium mongolicum regel extract, 2 parts of moutan bark extract, 5 parts of loquat leaf extract and 2 parts of honeysuckle extract.

Example 5

A plant extract composition with antibacterial and anti-inflammatory functions is prepared from the following raw materials in parts by weight: 5 parts of lotus plumule extract, 2 parts of allium mongolicum regel extract, 5 parts of moutan bark extract, 5 parts of loquat leaf extract and 2 parts of honeysuckle extract.

Example 6

A plant extract composition with antibacterial and anti-inflammatory functions is prepared from the following raw materials in parts by weight: 5 parts of lotus plumule extract, 2 parts of allium mongolicum regel extract, 2 parts of moutan bark extract, 5 parts of loquat leaf extract and 5 parts of honeysuckle extract.

Example 7

A plant extract composition with antibacterial and anti-inflammatory functions is prepared from the following raw materials in parts by weight: 5 parts of lotus plumule extract, 2 parts of allium mongolicum regel extract, 2 parts of moutan bark extract, 8 parts of loquat leaf extract and 5 parts of honeysuckle extract.

The plant extract compositions obtained in examples 1 to 7 were tested for the minimum inhibitory degree (MIC, unit: ppm) using potassium sorbate of equal weight as a control, and the results are shown in Table 1.

TABLE 1 results of bacteriostasis

As shown in table 1, the plant extract compositions obtained in examples 1 to 7 all have an outstanding bacteriostatic effect compared to potassium sorbate, the flavonoid compounds in the lotus plumule and the flavonoid compounds in allium mongolicum regel all improve the bacteriostatic effect of the compositions (examples 2 and 4), the addition of the active ingredients in the loquat leaves and the active ingredients in the honeysuckle extract (examples 3 and 5) remarkably improves the bacteriostatic effect of the compositions, and the combination of the extracts in example 7 has an optimal effect.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.