阅读说明：本技术 油田矿区历史遗留含油污泥微生物诱导原位深度处理方法 (Oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method ) 是由 王光义 樊勇杰 贾品元 张涛 陈少华 王新艳 李先勇 顾中波 于 2021-08-24 设计创作，主要内容包括：本发明属于污泥处理技术领域,具体涉及一种油田矿区历史遗留含油污泥微生物诱导原位深度处理方法,所述方法包括：制备乳酸菌菌液、芽孢杆菌菌液、石油降解菌菌液,上述各菌液混合后得到混合菌液；将所述混合菌液与秸秆粉以及表面活性剂相混合,干燥,制成负载有混合菌的菌粉；将井场历史遗留含油污泥挖出放置在预先做好防渗防泄漏的井场场地内,摊开；加入微生物菌粉后深翻耕,翻耕后的土壤表面洒水,静置3-7d；在土壤表面铺设一层秸秆段,发酵20-30d；在发酵完毕后的土壤中种植植物,每隔一个月补菌粉一次,直至土壤中石油含量达到排放要求。本发明将微生物与植物修复相结合,产生了很好的石油降解效果,具有较佳的应用前景。(The invention belongs to the technical field of sludge treatment, and particularly relates to an in-situ deep treatment method for historical legacy oily sludge microorganisms in an oil field mining area, which comprises the following steps: preparing lactobacillus bacteria liquid, bacillus bacteria liquid and petroleum degrading bacteria liquid, and mixing the bacteria liquids to obtain mixed bacteria liquid; mixing the mixed bacteria liquid with straw powder and a surfactant, and drying to prepare mixed bacteria-loaded bacteria powder; digging out the historical residual oily sludge of the well site, placing the oily sludge in the well site which is made with seepage prevention and leakage prevention in advance, and spreading the oily sludge; adding microbial powder, deep ploughing, spraying water on the ploughed soil surface, and standing for 3-7 d; laying a layer of straw sections on the soil surface, and fermenting for 20-30 d; and (4) planting plants in the fermented soil, and supplementing the bacterial powder once every other month until the petroleum content in the soil meets the emission requirement. The invention combines microorganism and plant restoration, generates good petroleum degradation effect and has better application prospect.)

1. The oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method is characterized by comprising the following steps:

s1, preparing bacterial powder:

preparing lactobacillus bacteria liquid, bacillus bacteria liquid and petroleum degrading bacteria liquid, and mixing the bacteria liquids to obtain mixed bacteria liquid;

mixing the mixed bacteria liquid with straw powder and a surfactant, and drying to prepare the mixed bacteria-loaded straw powder for later use;

s2, in-situ sludge transfer: digging out the historical residual oily sludge of the well site, placing the dug oily sludge in the well site with seepage prevention and leakage prevention and 50cm high cofferdams at the periphery, and spreading the dug oily sludge;

s3, primary fermentation: uniformly scattering bacterial powder on the soil surface for deep ploughing, sprinkling water on the soil surface after deep ploughing, and standing for 3-7 d;

s4, post-fermentation: laying a layer of straw sections on the soil surface, and fermenting for 20-30 d;

s5, phytoremediation: and (4) planting plants in the fermented soil, and supplementing the bacterial powder once every other month until the petroleum content in the soil meets the emission requirement.

2. The method for the microbial-induced in-situ advanced treatment of the oil-containing sludge left historically in the oilfield mine area according to claim 1, wherein the lactic acid bacteria are lactobacillus reuteri CGMCC NO.15528, the bacillus is bacillus megaterium CGMCC NO.3770, and the petroleum degrading bacteria are one or two of BDB-n biodegradable bacteria and BDB-a biodegradable bacteria.

3. The method for the in-situ deep treatment of the oil field mining area historical residual oily sludge through microorganism induction according to claim 2, wherein the viable count of the lactic acid bacteria liquid, the bacillus liquid and the petroleum degrading bacteria liquid is 10⁷Per mLThe mass ratio of the lactobacillus bacterial liquid to the bacillus bacterial liquid to the petroleum degrading bacterial liquid in the mixed bacterial liquid is 1:1: 1-2.

4. The method for the microorganism-induced in-situ deep treatment of the oily sludge left in the historical oil field mine area according to claim 3, wherein in S2, the spreading thickness of the oily sludge in the well site is 10-25cm, and the plowing depth is 25-40 cm.

5. The method for the microbial-induced in-situ advanced treatment of the historical residual oily sludge in the oilfield mining area according to claim 4, wherein in S3, the addition amount of the bacterial powder is 5-10kg per cubic meter of the oily sludge, and the water spraying amount is 1-2L per cubic meter of the oily sludge.

6. The method for the microbial-induced in-situ advanced treatment of the historical legacy oily sludge of the oilfield mine according to claim 5, wherein the straw is one or more of corn straw, wheat straw, sorghum straw and rice straw.

7. The method for the microbial-induced in-situ advanced treatment of the historical residual oily sludge of the oilfield mine according to claim 6, wherein the straw powder is prepared by crushing straws into straw powder with the length of 2-5 mm;

the straw sections are straw sections with the length of 2-5cm, and the laying thickness of the straw sections on the soil surface is 5-10 m.

8. The method for the microbial-induced in-situ advanced treatment of the historical legacy oily sludge in the oilfield mining area according to claim 7, wherein the bacterial powder is prepared by mixing bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:200: 3:

weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, mixing the bacteria liquid mixture with the straw mixture, and drying to obtain the bacteria powder.

9. The method of claim 8, wherein the surfactant is glycerol.

10. The method for the in-situ deep treatment of the oil field mining area historical residual oily sludge through microbial induction according to claim 1, wherein in S5, the plant is mixed seeds of corn grass, ryegrass or green bristlegrass which are mixed according to a mass ratio of 1:1: 1; the addition amount of the fungus powder is 0.2-0.5 times of the previous fungus powder.

Technical Field

The invention belongs to the technical field of sludge treatment, and particularly relates to an in-situ deep treatment method for historical legacy oily sludge microorganisms in an oil field mining area.

Background

The historical oily sludge of the oil field mining area/well site is the problem of the historical remaining environment of the mining area which is comprehensively formed in the early stage of the oil field development process due to the influence of technical process limitation and environmental protection attention degree, wherein the petroleum hydrocarbon and chemical agent residue cause greater risk to the mining area environment, and along with the improvement of the oil field development technical level and the increase of the treatment strength of China to the mining area environment, the historical remaining oily sludge of the oil field mining area/well site must be cleared and treated, the ecological function of polluted soil is recovered, and the harmony of high-quality development of an oil field and the mining area environment is realized. In the prior art.

The microorganism treatment method is a common oil-containing sludge treatment method at present, and selects microorganisms with the function of degrading organic matters, such as pseudomonas, rhodococcus, micrococcus, bacillus and the like with petroleum degradation capacity, wherein the pseudomonas is a straight or slightly bent gram-negative bacillus, has no cell nucleus, moves by polar flagella and does not form spores; the rhodococcus is a kind of aerobic gram-positive bacteria, can form a rudiment mycelium, does not move, and has rough or smooth bacterial colony; micrococcus is a gram-positive bacterium with spherical cells, rarely moves, does not produce spores, and has smooth bacterial colony; bacillus is a genus that produces spores that are particularly resistant to adverse conditions, and is resistant to many adverse environments. The microorganisms have good petroleum hydrocarbon degradation capability after being singly or mixed, and can be used for bioremediation of petroleum sludge or petroleum-polluted soil.

In the prior art, there are many technologies for degrading organic pollutants by using microorganisms, for example, chinese patent CN108580539A discloses a method for restoring organic polluted soil by coupling chemical oxidation with microorganisms, which sequentially adds ferrous sulfate activator solution, sodium persulfate oxidant solution, and potassium permanganate oxidant solution into the soil to be restored, and changes the community structure of the soil microorganisms by using chemical reagents, so as to realize in-situ efficient economic restoration of the organic polluted soil; for example, [ Zhangxianxiao, Baixuxuejing, Xuna, etc. ] research on influence factors of immobilized microorganism in repairing petroleum-polluted soil [ J ]. environmental engineering report, 2013(03): 1156-. These studies all show the function of microorganisms in degrading petroleum pollutants.

Although the method for degrading petroleum pollutants by microorganisms is long in time consumption, the operation is simple, the energy consumption of equipment is low, and new pollution is not easily caused, so that the method becomes a better sludge treatment mode, but a plurality of microorganism functions are degraded, and when the use times of the microorganism are more, the degradation capability of organic matters is reduced, so that a new method for treating sludge by microorganisms needs to be continuously developed.

Disclosure of Invention

In order to solve the technical problems, the invention provides a method for carrying out microorganism-induced in-situ advanced treatment on historical residual oily sludge in an oil field and a mining area, and belongs to a novel technology for carrying out microorganism in-situ advanced treatment on the oily sludge.

The invention aims to provide a microbial induction in-situ advanced treatment method for historical residual oily sludge in an oil field mining area, which comprises the following steps:

s1, preparing bacterial powder:

preparing lactobacillus bacteria liquid, bacillus bacteria liquid and petroleum degrading bacteria liquid, and mixing the bacteria liquids to obtain mixed bacteria liquid;

mixing the mixed bacteria liquid with straw powder and a surfactant, and drying to prepare the mixed bacteria-loaded straw powder for later use;

s2, in-situ sludge transfer: digging out the historical residual oily sludge of the well site, placing the dug oily sludge in the well site with seepage prevention and leakage prevention and 50cm high cofferdams at the periphery, and spreading the dug oily sludge;

s3, primary fermentation: uniformly scattering bacterial powder on the soil surface for deep ploughing, sprinkling water on the soil surface after deep ploughing, and standing for 3-7 d;

s4, post-fermentation: laying a layer of straw sections on the soil surface, and fermenting for 20-30 d;

s5, phytoremediation: and (4) planting plants in the fermented soil, and supplementing the bacterial powder once every other month until the petroleum content in the soil meets the emission requirement.

Preferably, the oil field mining area history legacy oily sludge microorganism induction in-situ deep treatment method comprises the step of carrying out deep treatment on the oil field mining area history legacy oily sludge by using lactobacillus reuteri CGMCC NO.15528, the bacillus is bacillus megaterium CGMCC NO.3770, and the petroleum degrading bacteria are one or two of BDB-n biodegradable bacteria and BDB-a biodegradable bacteria.

Preferably, in the oil field mining area history remaining oily sludge microorganism induction in-situ advanced treatment method, the number of viable bacteria in the lactic acid bacteria liquid, the bacillus liquid and the petroleum degrading bacteria liquid is more than 107/mL, and the mass ratio of the lactic acid bacteria liquid, the bacillus liquid and the petroleum degrading bacteria liquid in the mixed bacteria liquid is 1:1: 1-2.

Preferably, in the method for deep treatment of the oil field mining area historical residual oily sludge by microorganism induction in situ, in S2, the spreading and drying thickness of the oily sludge in the well site field is 10-25cm, and the plowing depth is 25-40 cm.

Preferably, in the method for deep treatment of historical residual oily sludge in oil field mining areas by microorganism induction in situ, in S3, the addition amount of the bacterial powder is 5-10kg per cubic meter of oily sludge, and the water sprinkling amount is 1-2L per cubic meter of oily sludge.

Preferably, the oil field mining area history legacy oily sludge microorganism induction in-situ advanced treatment method is adopted, and the straw is one or more of corn straw, wheat straw, sorghum straw and rice straw.

Preferably, in the oil field mining area history residual oily sludge microorganism induction in-situ advanced treatment method, the straw powder is obtained by crushing straws into straw powder with the length of 2-5 mm;

the straw sections are straw sections with the length of 2-5cm, and the laying thickness of the straw sections on the soil surface is 5-10 m.

Preferably, in the oil field mining area historical residual oily sludge microorganism induction in-situ advanced treatment method, the bacterial powder is prepared from mixed bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:200: 3:

weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, mixing the bacteria liquid mixture with the straw mixture, and drying to obtain the bacteria powder.

Preferably, the oil field mining area history residual oily sludge microorganism induction in-situ advanced treatment method is adopted, and the surfactant is glycerol.

Preferably, in the method for the in-situ deep treatment of the oil-containing sludge historical remnants in the oilfield, in step S5, the plant is a mixed seed obtained by mixing the grass seeds of corn grass, ryegrass or green bristlegrass according to a mass ratio of 1:1: 1; the addition amount of the fungus powder is 0.2-0.5 times of the previous fungus powder.

Compared with the prior art, the invention has the following beneficial effects:

1. the invention selects lactobacillus reuteri CGMCC NO.15528, bacillus megaterium CGMCC NO.3770, BDB-n biodegradable bacteria and BDB-a biodegradable bacteria with petroleum degradation function, combines the microorganisms with plant repair, generates good petroleum degradation effect and has better application prospect.

2. The method disperses redundant petroleum pollutants in the oily sludge by utilizing the viscosity of the soil in the well site field, so that the content of petroleum in the sludge is reduced; soil in the well site field provides a nutrient medium for the growth of subsequent plants; standing for 3-7 days during primary fermentation to allow the microorganisms to penetrate into the soil and propagate, wherein the microorganisms have a primary degradation effect on oil stains in the soil; during post-fermentation, straw sections are added for fermentation for 20-30d, the straw sections have the functions of preserving heat and moisture and providing proper temperature and humidity for fermentation of microorganisms, and the straws after being decomposed by the microorganisms can improve soil quality and increase the content of soil nutrient elements; the microbial powder is added in the plant repairing process, and the petroleum degradation effect can be enhanced together with the plant.

3. The invention designs a special preparation method of microbial bacteria powder, which comprises the steps of dividing a surfactant into two parts with equal mass, fully mixing one part of the surfactant with a mixed bacterial liquid to obtain a bacterial liquid mixture, mixing the other part of the surfactant with straw powder to obtain a straw mixture, changing the surface polarity of the mixed bacterial liquid added with the surfactant and the straw powder, and mixing the bacterial liquid mixture with the straw mixture, wherein bacteria can be quickly adsorbed on the straw powder.

4. The petroleum sludge treated by the method can be used as a base fertilizer or a soil improvement matrix for improving the soil nutrition of barren fields, and the planted herbaceous crops can be used as greening environments.

Drawings

FIG. 1 is a flow chart of the method for the microorganism-induced in-situ advanced treatment of the historical residual oily sludge in the mining area of the oil field.

Detailed Description

In order that those skilled in the art will better understand the technical solutions of the present invention to be implemented, the present invention will be further described with reference to the following specific embodiments and accompanying drawings.

In the description of the present invention, reagents used are commercially available and methods used are conventional in the art, unless otherwise specified. The lactobacillus reuteri CGMCC NO.15528 and the bacillus megaterium CGMCC NO.3770 are purchased from China general microbiological culture Collection center (CGMCC); the BDB-n biodegradable bacteria and the BDB-a biodegradable bacteria are sold in the market and are products developed by the Nanyang Donghua company and the Beijing university environmental college; the above-mentioned method for the expanded culture of each bacterial suspension was carried out by a conventional method, or commercially available bacterial suspensions were directly purchased. The oily sludge used in the invention is from an oil field well site of prolonged petroleum, and the heavy metal content in the sludge-containing sample used in the invention meets the agricultural sludge pollutant control standard GB 4284-2018.

It should be noted that, in the present invention, all the test data are randomly sampled and measured three times respectively, and are expressed in the form of "mean ± standard deviation".

The invention provides a microbial-induced in-situ advanced treatment method for historical residual oily sludge in an oil field mining area, which comprises the following embodiments.

Example 1

An oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method comprises the following steps:

s1, preparing a lactic acid bacteria liquid, a bacillus liquid and a petroleum degrading bacteria liquid, and mixing the above bacteria liquids to obtain a mixed bacteria liquid for later use; the lactobacillus is Lactobacillus reuteri CGMCC NO.15528, and the concentration of the bacteria liquid is 3 x 10⁷Each mL, the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 6 multiplied by 10⁷each/mL, the petroleum degrading bacteria are BDB-n biodegradable bacteria, and the concentration of the bacteria liquid is 5 multiplied by 10⁷Per mL; the mass ratio of the lactobacillus bacterial liquid to the bacillus bacterial liquid to the petroleum degrading bacterial liquid is 1:1: 2;

the bacterial powder is prepared from mixed bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:200: 3:

weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;

the straw powder is formed by crushing dried wheat straws into straw powder with the length of 2 mm; the surfactant is glycerol;

s2, in-situ sludge transfer: will be 20m³Digging out the oil-containing sludge which is historically left in the well site, placing the oil-containing sludge in the well site with the functions of seepage prevention, leakage prevention and 50cm high cofferdams at the periphery, and spreading out the oil-containing sludge, wherein the spreading thickness of the oil-containing sludge on the soil is 15 cm;

s3, primary fermentation: uniformly scattering 100kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 25 cm; the plowed sludge is basically and uniformly mixed with the field soil of the well site, and the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 8.67 +/-0.29 g/100 g; spraying 20L of water on the surface of the deeply ploughed soil, and standing for 3 d;

s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the soil surface, and fermenting for 30 d; the straw section is formed by crushing dried wheat straws into straw sections with the length of 5 cm; sampling and testing the oil content in the soil with the depth of 10cm to be 3.72 +/-0.07 g/100g, and the oil removal rate is ((8.67-3.72)/8.67) × 100 percent to 57.09 percent;

s5, phytoremediation: and planting plants in the fermented soil, uniformly spraying 20kg of bacterial powder every other month, and sampling and testing the oil content of the soil with the depth of 10cm to be 1.12 +/-0.19 g/100g and the oil removal rate to be ((8.67-1.12)/8.67). times.100 percent to 87.08 percent after one month.

Example 2

An oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method comprises the following steps:

s1, preparing a lactic acid bacteria liquid, a bacillus liquid and a petroleum degrading bacteria liquid, and mixing the above bacteria liquids to obtain a mixed bacteria liquid for later use; the lactobacillus is Lactobacillus reuteri CGMCC NO.15528, and the concentration of the bacteria liquid is 3 x 10⁷Each mL, the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 6 multiplied by 10⁷Each mL, the petroleum degrading bacteria are BDB-a biodegradable bacteria, and the concentration of the bacteria liquid is 6 multiplied by 10⁷Per mL; the mass ratio of the lactobacillus bacterial liquid to the bacillus bacterial liquid to the petroleum degrading bacterial liquid is 1:1: 2;

the bacterial powder is prepared from mixed bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:200: 3:

weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;

the straw powder is straw powder which is formed by crushing dried corn straws and has the length of 5 mm; the surfactant is glycerol;

s2, in-situ sludge transfer: will be 20m³Digging out the oil-containing sludge which is historically left in the well site, placing the oil-containing sludge in the well site with the functions of seepage prevention, leakage prevention and 50cm high cofferdams at the periphery, and spreading the oil-containing sludge out of the soil to a spreading thickness of 25 cm;

s3, primary fermentation: uniformly spreading 200kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 40 cm; the plowed sludge is basically and uniformly mixed with the well site field soil, the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 6.43 +/-0.19 g/100g, 40L of water is sprayed on the surface of the deeply plowed soil, and then the mixture is kept still for 7 d;

s4, post-fermentation: laying a layer of straw sections with the thickness of 10cm on the soil surface, and fermenting for 20 d; the straw section is formed by crushing dried corn straws into straw sections with the length of 2 cm; sampling and testing the oil content in the soil with the depth of 10cm to be 3.57 +/-0.10 g/100g, and the oil removal rate is ((6.43-3.57)/6.43) × 100 percent to 44.48 percent;

s5, phytoremediation: after the fermentation is finished, plants are planted in the soil, 60kg of bacterial powder is evenly sprayed every other month, after one month, sampling and testing are carried out, the oil content in the soil with the depth of 10cm is 0.84 +/-0.04 g/100g, and the oil removal rate is ((6.43-0.84)/6.43). times.100 percent-86.94 percent.

Example 3

An oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method comprises the following steps:

s1, preparing a lactic acid bacteria liquid, a bacillus liquid and a petroleum degrading bacteria liquid, and mixing the above bacteria liquids to obtain a mixed bacteria liquid for later use; the lactobacillus is Lactobacillus reuteri CGMCC NO.15528, and the concentration of the bacteria liquid is 3 x 10⁷Each mL, the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 6 multiplied by 10⁷The petroleum degrading bacteria are a mixture of BDB-n biodegradable bacteria and BDB-a biodegradable bacteria according to the mass ratio of 1:1, and the concentration of BDB-n biodegradable bacteria solution is 5 multiplied by 10⁷The bacterial concentration of the BDB-a biodegradable bacteria is 6 multiplied by 10⁷Per mL; the mass ratio of the lactobacillus bacterial liquid to the bacillus bacterial liquid to the petroleum degrading bacterial liquid is 1:1: 2;

the bacterial powder is prepared from mixed bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:200: 3:

weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;

the straw powder is prepared by crushing dry sorghum straws into straw powder with the length of 4 mm; the surfactant is glycerol;

s2, in-situ sludge transfer: will be 20m³Digging out the oil-containing sludge which is historically left in the well site, placing the oil-containing sludge in the well site with the functions of seepage prevention, leakage prevention and 50cm high cofferdams at the periphery, and spreading out the oil-containing sludge, wherein the spreading thickness of the oil-containing sludge on the soil is 10 cm;

s3, primary fermentation: uniformly scattering 140kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 25 cm; the plowed sludge is basically and uniformly mixed with the field soil of the well site, and the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 8.30 +/-0.16 g/100 g; spraying 30L of water on the surface of the deeply ploughed soil, and standing for 5 d;

s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the soil surface, and fermenting for 25 d; the straw section is formed by crushing dried sorghum straws into straw sections with the length of 5 cm; sampling soil with 10cm depth has oil content of 4.00 + -0.12 g/100g, oil removal rate of (8.30-4.00)/8.30) × 100% ═ 51.80%;

s5, phytoremediation: after fermentation, plants are planted in the soil, 42kg of bacterial powder is uniformly sprayed every other month, and after one month, sampling and testing are carried out, wherein the oil content in the soil with the depth of 10cm is 0.94 +/-0.03 g/100g, and the oil removal rate is ((8.30-0.94)/8.30). times.100 percent, namely 88.67 percent.

Example 4

An in-situ induction advanced treatment method for historical legacy oily sludge microorganisms in an oil field mining area comprises the following steps:

s1, preparing a lactic acid bacteria liquid, a bacillus liquid and a petroleum degrading bacteria liquid, and mixing the above bacteria liquids to obtain a mixed bacteria liquid for later use; the lactobacillus is Lactobacillus reuteri CGMCC NO.15528, and the concentration of the bacteria liquid is 3 x 10⁷Each mL, the bacillus is bacillus megaterium CGMCC NO.3770, the concentration of the bacterial liquid is 6 multiplied by 10⁷each/mL, the petroleum degrading bacteria are BDB-n biodegradable bacteria, and the concentration of the bacteria liquid is 5 multiplied by 10⁷Per mL; the mass ratio of the lactobacillus bacterial liquid to the bacillus bacterial liquid to the petroleum degrading bacterial liquid is 1:1: 3;

the bacterial powder is prepared from mixed bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:200: 3:

weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;

the straw powder is prepared by crushing dry rice straws into straw powder with the length of 2 mm; the surfactant is glycerol;

s2, in-situ sludge transfer: will be 20m³Digging out the oil-containing sludge which is historically left in the well site, placing the oil-containing sludge in the well site with the functions of seepage prevention, leakage prevention and 50cm high cofferdams at the periphery, and spreading out the oil-containing sludge, wherein the spreading thickness of the oil-containing sludge on the soil is 15 cm;

s3, primary fermentation: uniformly scattering 200kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 25 cm; the plowed sludge is basically and uniformly mixed with the field soil of the well site, and the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 8.19 +/-0.26 g/100 g; spraying 20L of water on the surface of the deeply ploughed soil, and standing for 3 d;

s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the soil surface, and fermenting for 30 d; the straw sections are straw sections with the length of 5cm which are obtained by crushing dry rice straws; sampling and testing the oil content in the soil with the depth of 10cm to be 3.48 +/-0.06 g/100g, and the oil removal rate is ((8.19-3.48)/8.19) multiplied by 100 percent which is 57.51 percent;

s5, phytoremediation: planting plants in the fermented soil, uniformly spraying 100kg of bacterial powder every other month, and sampling and testing the oil content of the soil with the depth of 10cm to be 0.76 +/-0.03 g/100g and the oil removal rate to be ((8.19-0.76)/8.19). times.100 percent to be 90.72 percent after one month.

Experimental example 1

An oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method comprises the following steps:

s1, preparing lactobacillus bacterial liquid for later use; the lactobacillus is Lactobacillus reuteri CGMCC NO.15528, and the concentration of the bacteria liquid is 3 x 10⁷The amount of the lactobacillus in the experimental example is the same as that in the example 1, and the bacteria solution of the bacillus and the petroleum degrading bacteria in the example 1 is replaced by the sterile water with equal mass;

the bacterium powder is prepared from lactobacillus bacterium liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:200: 3:

weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;

the straw powder is formed by crushing dried wheat straws into straw powder with the length of 2 mm; the surfactant is glycerol;

s2, in-situ sludge transfer: will be 20m³Digging out the oil-containing sludge which is historically left in the well site, placing the oil-containing sludge in the well site with the functions of seepage prevention, leakage prevention and 50cm high cofferdams at the periphery, and spreading out the oil-containing sludge, wherein the spreading thickness of the oil-containing sludge on the soil is 15 cm;

s3, primary fermentation: uniformly scattering 100kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 25 cm; the plowed sludge is basically and uniformly mixed with the field soil of the well site, and the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 8.75 +/-0.23 g/100 g; spraying 20L of water on the surface of the deeply ploughed soil, and standing for 3 d;

s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the soil surface, and fermenting for 30 d; the straw section is formed by crushing dried wheat straws into straw sections with the length of 5 cm; sampling and testing the oil content in soil with the depth of 10cm to be 8.00 +/-0.16 g/100g, and the oil removal rate is ((8.75-8.00)/8.75) × 100 percent to 8.57 percent;

s5, phytoremediation: and planting plants in the fermented soil, uniformly spraying 20kg of bacterial powder every other month, and sampling and testing the oil content of the soil with the depth of 10cm to be 7.52 +/-0.13 g/100g and the oil removal rate to be ((8.75-7.52)/8.75). times.100 percent to be 14.06 percent after one month.

Experimental example 2

An oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method comprises the following steps:

s1, preparing a bacillus liquid for later use; the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 6 multiplied by 10⁷Each mL, the using amount of the lactic acid bacteria in the experimental example is the same as that of the lactic acid bacteria in the example 1, and sterile water with equal mass is used for replacing the lactic acid bacteria and the petroleum degrading bacteria liquid in the example 1;

the bacterial powder is prepared from bacillus bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:200: 3:

weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;

the straw powder is formed by crushing dried wheat straws into straw powder with the length of 2 mm; the surfactant is glycerol;

s2, in-situ sludge transfer: will be 20m³Digging out the oil-containing sludge which is historically left in the well site, placing the oil-containing sludge in the well site with the functions of seepage prevention, leakage prevention and 50cm high cofferdams at the periphery, and spreading out the oil-containing sludge, wherein the spreading thickness of the oil-containing sludge on the soil is 15 cm; (ii) a

S3, primary fermentation: uniformly scattering 100kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 25 cm; the plowed sludge is basically and uniformly mixed with the field soil of the well site, and the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 8.45 +/-0.39 g/100 g; spraying 20L of water on the surface of the deeply ploughed soil, and standing for 3 d;

s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the soil surface, and fermenting for 30 d; the straw section is formed by crushing dried wheat straws into straw sections with the length of 5 cm; sampling and testing the oil content in soil with the depth of 10cm to be 7.82 +/-0.09 g/100g, and the oil removal rate is ((8.45-7.82)/8.45) × 100 percent to be 7.46 percent;

s5, phytoremediation: and (3) planting plants in the fermented soil, uniformly spraying 20kg of bacterial powder every other month, and sampling and testing the oil content of the soil with the depth of 10cm to be 7.03 +/-0.06 g/100g and the oil removal rate to be ((8.45-7.03)/8.45). times.100% to be 16.80% after one month.

Experimental example 3

An oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method comprises the following steps:

s1, preparing petroleum degrading bacteria liquid for later use; the petroleum degrading bacteria are BDB-n biodegradable bacteria, and the concentration of the bacteria liquid is 5 multiplied by 10⁷Per mL; in the experimental example, the amount of the petroleum degrading bacteria is the same as that of the petroleum degrading bacteria in the example 1, and sterile water with equal mass is used for replacing the lactobacillus bacteria liquid and the bacillus bacteria liquid in the example 1;

the bacterial powder is prepared from bacillus bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:200: 3:

weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;

the straw powder is formed by crushing dried wheat straws into straw powder with the length of 2 mm; the surfactant is glycerol;

s2, in-situ sludge transfer: will be 20m³Digging out the oil-containing sludge left in the well site history, placing the well site in a well site field with a pre-made anti-seepage and anti-leakage cofferdam with the height of 50cm around, spreading out the oil-containing sludge on the soilThe spreading and drying thickness of the cloth is 15 cm;

s3, primary fermentation: uniformly scattering 100kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 25 cm; (ii) a The plowed sludge is basically and uniformly mixed with the field soil of the well site, and the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 8.33 +/-0.11 g/100 g; spraying 20L of water on the surface of the deeply ploughed soil, and standing for 3 d;

s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the soil surface, and fermenting for 30 d; the straw section is formed by crushing dried wheat straws into straw sections with the length of 5 cm; sampling and testing the oil content in soil with the depth of 10cm to be 6.51 +/-0.15 g/100g, and the oil removal rate is ((8.33-6.51)/8.33) × 100 percent to 21.85 percent;

s5, phytoremediation: and planting plants in the fermented soil, uniformly spraying 20kg of bacterial powder every other month, and sampling and testing the oil content of the soil with the depth of 10cm to be 4.29 +/-0.15 g/100g and the oil removal rate to be ((8.33-4.29)/8.33). times.100 percent to 48.50 percent after one month.

In the above examples and experimental examples, the oil content of the soil in the depth of 10cm in the plowed sludge is different from that of the soil in the well site field, and is influenced by the plowing depth and the oil content of the soil in the well site field.

It should be noted that, when the present invention relates to a numerical range, it should be understood that two endpoints of each numerical range and any value between the two endpoints can be selected, and since the steps and methods adopted are the same as those in the embodiment, in order to prevent redundancy, the present invention describes a preferred embodiment. While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.

It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.