阅读说明：本技术 一种控油抗炎的植物组合提取物及其制备方法和应用 (Oil-control anti-inflammatory plant combined extract and preparation method and application thereof ) 是由 亓云吉 于 2020-12-22 设计创作，主要内容包括：本申请提供了一种控油抗炎的植物组合提取物,所述植物组合提取物采用包括以下质量份数的原料制备获得：油橄榄叶15～45份,青叶胆15～45份,女贞果15～45份,黄连4～25份,当归4～25份,甘草4～25份。本申请还提供了上述植物组合提取物的制备方法和应用。本申请提供的植物组合提取物,采用特定种类和用量配比的组分原料进行混合提取,所得产物在应用于护肤品时,具有明显提升的即时控油效果和长效控油效果,控油起效快且不易反复,相较于常见的控油植物提取物具有显著的控油优势,并且能够有效抑制I型5α-还原酶和II型5α-还原酶,同时抑制因皮肤毛囊中的厌氧菌痤疮杆菌滋生而过度表达的IL-1β、TNF-α和COX-2炎症因子,实现标本兼治的效果。(The application provides an oil-control anti-inflammatory plant combined extract, which is prepared from the following raw materials in parts by mass: 15-45 parts of olive leaves, 15-45 parts of swertia mileensis, 15-45 parts of privet fruits, 4-25 parts of coptis chinensis, 4-25 parts of angelica sinensis and 4-25 parts of liquorice. The application also provides a preparation method and application of the plant combined extract. The plant combination extract provided by the application is prepared by mixing and extracting component raw materials of specific types and dosage ratios, and when the obtained product is applied to a skin care product, the obtained product has an obviously improved instant oil control effect and a long-acting oil control effect, the oil control effect is quick and not easy to repeat, and compared with a common oil control plant extract, the plant combination extract has a remarkable oil control advantage, can effectively inhibit I-type 5 alpha-reductase and II-type 5 alpha-reductase, and simultaneously inhibits over-expressed IL-1 beta, TNF-alpha and COX-2 inflammatory factors caused by breeding of anaerobic bacteria acnes in skin hair follicles, so that the effect of treating both symptoms and root causes is realized.)

1. The oil-control anti-inflammatory plant combined extract is characterized by being prepared from the following raw materials in parts by mass: 15-45 parts of olive leaves, 15-45 parts of swertia mileensis, 15-45 parts of privet fruits, 4-25 parts of coptis chinensis, 4-25 parts of angelica sinensis and 4-25 parts of liquorice.

2. The oil-control anti-inflammatory plant combined extract as claimed in claim 1, wherein the plant combined extract is prepared from the following raw materials in parts by mass: 20-40 parts of olive leaves, 20-40 parts of swertia mileensis, 20-40 parts of privet fruits, 5-20 parts of coptis chinensis, 5-10 parts of angelica sinensis and 5-10 parts of liquorice.

3. A method for preparing the oil-controlling anti-inflammatory botanical combined extract as claimed in claim 1 or 2, comprising the steps of:

step (1): weighing olive leaves, swertia mileensis and privet fruits according to the mass ratio, mixing and crushing, and extracting by using organic alcohol to obtain a first extracting solution;

step (2): weighing coptis chinensis, angelica sinensis and liquorice according to the mass ratio, extracting with water to obtain a second extracting solution, filtering the second extracting solution, concentrating the filtrate, precipitating with ethanol, and concentrating under reduced pressure to obtain a first clear paste;

and (3): mixing the first extract obtained in the step (1) and the first clear paste obtained in the step (2), stirring, standing, filtering, and concentrating under reduced pressure to obtain a second clear paste;

and (4): adding 1, 3-butanediol into the second clear paste obtained in the step (3) and uniformly mixing to obtain the oil-controlling anti-inflammatory plant combined extract.

4. The method as claimed in claim 3, wherein the ratio of the organic alcohol extracted in step (1) to the liquid is 1 g: (10-20) ml, wherein the organic alcohol is ethanol with the volume concentration of 65% -90%.

5. The method of claim 3, wherein the alcohol precipitation step of step (2) comprises: adding ethanol into the concentrated filtrate to ensure that the alcohol content reaches 48-55%, stirring and standing.

6. The method according to claim 3, wherein the second fluid extract obtained in step (3) has a relative density of 1.3-1.5 measured at 50 ℃.

7. The method according to claim 3, wherein in the step (4), the mass part ratio of the second fluid extract to the 1, 3-butanediol is (1-5): (2-9).

8. Use of the oil-controlling and anti-inflammatory plant combination extract according to claim 1 or 2 and/or the oil-controlling and anti-inflammatory plant combination extract prepared by the method according to any one of claims 3 to 7 in the preparation of 5 α -reductase inhibitor products, inflammatory factor inhibitor products, acne prevention and treatment medicines, alopecia prevention products and/or oil-controlling and anti-inflammatory skin care products;

preferably, the dosage form of the 5 alpha-reductase inhibitor product, the inflammatory factor inhibitor product and/or the acne-inhibiting medicament includes, but is not limited to, external application liquid or cream; the dosage forms of the anti-hair loss product and/or the oil-control anti-inflammatory skin care product include, but are not limited to, aqua, cream, emulsion, essence and gel.

9. An oil-control anti-inflammatory skin care product, which comprises the oil-control anti-inflammatory plant combination extract as defined in claim 1 or 2 and/or the oil-control anti-inflammatory plant combination extract prepared by the method as defined in any one of claims 3 to 7, and is characterized in that the oil-control anti-inflammatory skin care product comprises the following components in percentage by mass: 0.1-10% of the oil-controlling anti-inflammatory plant combined extract;

preferably, the oil-control anti-inflammatory skin care product further comprises an auxiliary material, wherein the auxiliary material is one or more selected from water, glycerol, 1, 3-butanediol, sodium hyaluronate, betaine, xanthan gum, an emulsifier, an emollient, a humectant and an antioxidant.

10. A method of preparing the oil-controlling anti-inflammatory skin care product of claim 9, comprising the steps of:

step a: dissolving 1, 3-butanediol, glycerol, xanthan gum, sodium hyaluronate and betaine in water, heating to 75-85 ℃, and uniformly stirring to obtain a phase A;

step b: mixing an emulsifier and an emollient, heating to 75-85 ℃, and uniformly stirring to obtain a phase B;

step c: dissolving a humectant and an antioxidant in water to obtain phase C;

step d: mixing the phase B and the phase A, homogenizing at 75-85 ℃ and 3000-4000 r/min for 4-10 min, and cooling at 20-30 r/min at the speed of 1-3 ℃/min; and when the temperature is reduced to 45 ℃, adding the phase C and the oil-control anti-inflammatory plant combined extract, continuously stirring, and cooling to below 40 ℃ to obtain the oil-control anti-inflammatory skin care product.

Technical Field

The application relates to the technical field of fine chemical and daily necessities, in particular to an oil-control anti-inflammatory plant combined extract and a preparation method and application thereof.

Background

Oily skin is produced by the hypolipidemic gland of the human body with vigorous secretion and dysfunction. The sebaceous gland is an important gland attached to the skin and is an important site for producing sebum. In people with oily skin, sebaceous glands secrete more than constant oil, and excessive oil can cause oil on the surface of the skin, easily contaminate the environment, and simultaneously cause corresponding skin diseases, such as skin sensitivity, allergy, acne, seborrheic dermatitis and the like, and also cause seborrheic alopecia and the like. Scientific research finds that the causes of the dysfunction of sebaceous glands are complex, and the formed lesions are diversified, wherein the main factors have the following effects: endocrine hormones, age, lifestyle habits, impaired skin barrier and outside climate, etc.

In response to sebaceous gland dyssecretosis, generally medically in response to hyperandrogenism, the use of increasing estrogen regulation or the use of some drugs such as cimetidine tincture against androgens that cause sebaceous gland dyssecretosis, and the use of finasteride, dutasteride and epristeride to inhibit 5 α -reductase. However, the use of hormonal drugs has various physical injuries, and finasteride, dutasteride and epristeride in particular affect the fertility of men. Therefore, the safety of the 5 α -reductase inhibitory drugs of the prior art has yet to be improved.

For nursing oily skin, the main purposes are that the skin is clean and thorough, the secretion of grease is controlled, and the skin is in a water-oil balance state as much as possible. Meanwhile, the anti-inflammatory and antibacterial work is well done, and the skin diseases such as acne and seborrheic dermatitis can be effectively assisted and prevented and controlled.

At present, oil-control skin care products in the market usually use oil-absorbing powder (such as silicon dioxide, titanium dioxide, talc and the like) or plant extracts as oil-control active ingredients, wherein the oil-absorbing powder is used for quickly filling pores by using powder particles and absorbing redundant oil so as to improve the problem of skin glossing, but the use of the oil-absorbing powder only temporarily reduces the gloss of the skin, can not treat both symptoms and root causes, and even excessive addition can cause the problems of pore blockage and the like; however, the oil-controlling plant extracts commonly used at present, such as hamamelis virginiana, calendula officinalis, aloe, philippine violet herb and the like, have the problems of slow effect, short effective time, easy repetition and the like although the side effects are small, and the oil-controlling anti-inflammatory effect is not ideal.

Patent CN110538088A provides a skin care composition, which aims at the problem that acid or protease functional components with strong hydrophilicity only act on the surface layer of the skin, but penetrate into hair follicles to have weak effect so as to have poor oil control effect, and provides a skin care product in a pure oil dosage form. However, the skin care composition is prepared by mixing a plurality of functional components, does not have the natural and broader-spectrum effects of plant extracts, and the lipophilic skin care product has poor air permeability and skin affinity compared with the hydrophilic functional components, which is not beneficial to improving the use experience.

Disclosure of Invention

In order to solve the problems, the technical route of controlling oil and inhibiting inflammatory factors to achieve anti-inflammation and bacteriostasis by inhibiting 5 alpha-reductase is adopted, and the theoretical thought of the traditional Chinese medicine is utilized to provide the plant combination extract with obvious rapid and long-acting oil control, anti-inflammation and bacteriostasis, and the preparation method and the application of the plant combination extract.

In one aspect, the application provides an oil-controlling and anti-inflammatory plant combined extract, which is prepared from the following raw materials in parts by mass: 15-45 parts of olive leaves, 15-45 parts of swertia mileensis, 15-45 parts of privet fruits, 4-25 parts of coptis chinensis, 4-25 parts of angelica sinensis and 4-25 parts of liquorice.

Further, the plant combined extract is prepared from the following raw materials in parts by mass: 20-40 parts of olive leaves, 20-40 parts of swertia mileensis, 20-40 parts of privet fruits, 5-20 parts of coptis chinensis, 5-10 parts of angelica sinensis and 5-10 parts of liquorice.

In one embodiment, the plant combination extract may be an extract mixture obtained by extracting the above-mentioned raw materials respectively to obtain extracts and then mixing them, or may be a mixed extract obtained by mixing the above-mentioned raw materials and then extracting them. Preferably, the plant combination extract is obtained by mixing part of raw materials and then extracting to obtain an extract I, and then mixing the extract I with the rest of raw materials and then extracting to obtain an extract II.

In the above raw materials for preparing the plant composite extract:

the olive leaf is the leaf of olive tree, the active components in the extract mainly include oleuropein and hydroxytyrosol, and has antioxidant effect, and the oleanolic acid component has effects of inhibiting 5 alpha-reductase and regulating cell division and proliferation;

the swertia mileensis is a dried plant with the whole plant part used as the traditional Chinese medicinal material, and the oleanolic acid component contained in the swertia mileensis has the effects of inhibiting 5 alpha-reductase and regulating cell division and proliferation;

the glossy privet fruit is the dry fruit of glossy privet tree, and the oleanolic acid, maslinic acid and ursolic acid contained in the glossy privet fruit have the oil control effect;

the part of the Chinese goldthread used as the traditional Chinese medicine is underground dry stem block, and the berberine component contained in the Chinese goldthread has the functions of inhibiting acne bacillus and improving or controlling skin allergy symptom;

the part of the liquorice used as the traditional Chinese medicine is dry roots and rhizomes, and the reaction of the glycyrrhetinic acid, the glycyrrhizic acid and the glycyrrhizic acid salt contained in the liquorice to the inflammatory factors is inhibited;

the Chinese angelica is used as a Chinese medicinal material, the using parts of the Chinese angelica are dry roots and rhizomes, and the contained polysaccharide, volatile oil and vitamin have the auxiliary help function of improving the oxidation resistance and regulating the immune system of the skin and the function of enhancing the barrier function of the skin.

Therefore, the oil-control anti-inflammatory effect achieved by the plant combined extract provided by the application is the result of the combined action of the combined extract obtained by mixing and extracting all the raw materials.

On one hand, the plant combination extract provided by the application can show a remarkable oil-control anti-inflammatory effect, and plays a common role in multiple aspects of oil control, anti-inflammation, bacteriostasis, antioxidation, skin barrier repair and the like, so that the effects of conditioning the skin and improving skin problems of oily skin can be achieved.

On the other hand, compared with common oil control plant extracts used in the existing skin care products, the effects of instant oil control and long-acting oil control are more remarkable; moreover, the plant combined extract utilizes acid substances with good hydrophilicity (such as oleanolic acid, maslinic acid and ursolic acid) to inhibit 5 alpha-reductase so as to achieve the oil control effect, but it can exhibit the advantages of fast onset of oil control and long oil control time, which is not easy to repeat, because of the assistance of other components in the raw material, for example, the radix Angelicae sinensis extract contains a large amount of radix Angelicae sinensis volatile oil including neutral oil, phenolic oil and acidic oil, the angelica volatile oil has the functions of inhibiting inflammation and activating blood circulation, and can assist the acid substances for controlling oil to penetrate into the hair follicle, thereby realizing long-acting oil control, solving the problems that the existing plant extract or the plant extract containing the acid substance effective components has good hydrophilicity, naturalness and skin-friendly property, however, the oil control effect is difficult to further improve, which is also the result of the combined action of the extracts extracted from all the raw materials.

In addition, compared with a mixture obtained by mixing various single functional components, the plant combination extract provided by the application has more natural components, wider action and synergistic effect of interaction, and particularly can obviously enhance the prevention and improvement effects of the medicine or the skin care product when the acne-inhibiting medicine and/or the oil-controlling anti-inflammatory skin care product is prepared.

In another aspect, the present application also provides a method for preparing the above oil-controlling anti-inflammatory botanical combined extract, comprising the steps of:

step (1): weighing olive leaves, swertia mileensis and privet fruits according to the mass ratio, mixing and crushing, and extracting by using organic alcohol to obtain a first extracting solution;

step (2): weighing coptis chinensis, angelica sinensis and liquorice according to the mass ratio, extracting with water to obtain a second extracting solution, filtering the second extracting solution, concentrating the filtrate, precipitating with ethanol, and concentrating under reduced pressure to obtain a first clear paste;

and (3): mixing the first extract obtained in the step (1) and the first clear paste obtained in the step (2), stirring, standing, filtering, and concentrating under reduced pressure to obtain a second clear paste;

and (4): adding 1, 3-butanediol into the second clear paste obtained in the step (3) and uniformly mixing to obtain the oil-controlling anti-inflammatory plant combined extract.

In the conventional traditional Chinese medicine extraction method in the prior art, the raw materials are usually only uniformly mixed and then repeatedly extracted by using a solvent, but in the preparation method provided by the application, ethanol-insoluble impurities are removed by an ethanol precipitation step after water extraction is carried out in the step (2), and the first extracting solution and the second clear paste are mixed in the step (3) and then continuously stand and concentrated, which is equivalent to carrying out secondary ethanol precipitation. The process of twice alcohol precipitation can effectively improve the extraction rate of active ingredients in the traditional Chinese medicine raw materials and the content and purity of the active ingredients in the extract, and can also improve the viscosity of the second clear paste, so that the second clear paste is suitable for preparation of various dosage forms, otherwise, the prepared final extract is thinner in texture, and can be thickened only by increasing the content of the thickening agent in subsequent preparation, and the process is not beneficial to improving the content of the active ingredients when products with the same quality or volume are prepared.

And adding 1, 3-butanediol into the second fluid extract to serve as a solvent of the second fluid extract. On one hand, the second clear paste is prepared into a dosage form and is mixed with 1, 3-butanediol to further increase the viscosity, which is beneficial to subsequently preparing various dosage forms, and can increase the content of active ingredients and reduce the dosage of auxiliary materials under the same volume or mass; on the other hand, the use of the 1, 3-butanediol can also improve the moisturizing effect, maintain the hydrophilicity of the plant composition, be beneficial to assisting the plant composition extract to play a role in long-acting oil control, and in addition, the 1, 3-butanediol can also play a role in assisting the active ingredients of the extract to permeate into the skin.

Further, in the step (1) and the step (2), the number of times of extraction is at least two, preferably 2 to 5 times, and the extraction solutions are combined after the plurality of times of extraction.

Further, the extraction is heating reflux extraction.

Further, the ratio of the organic alcohol extracted in the step (1) to the liquid is 1 g: (10-20) ml, wherein the organic alcohol is ethanol with the volume concentration of 65% -90%.

Wherein, when the ethanol with the concentration is extracted according to the material-liquid ratio, oleanolic acid, maslinic acid and ursolic acid in olive leaves, swertia mileensis and privet fruits can be fully extracted, which is beneficial to exerting the oil control effect.

Further, the amount of water used in the step (2) is 5-30 times, preferably 10 times of the mass of the raw materials.

Further, the alcohol precipitation step of step (2) comprises: adding ethanol into the concentrated filtrate to ensure that the alcohol content reaches 48-55%, stirring and standing.

Wherein, the coptis root, the angelica and the liquorice are extracted by the water with the above dosage, and berberine in the coptis root, antioxidant and polysaccharide substances in the angelica, glycyrrhetinic acid, glycyrrhizic acid, glycyrrhetate and the like in the liquorice can be fully extracted. And the ethanol precipitation mode is adopted after the water extraction, so that the ethanol insoluble impurities can be removed again, and the extraction efficiency and purity can be improved.

Further, the relative density of the second clear paste obtained in the step (3) measured at 50 ℃ is 1.3-1.5.

Further, in the step (4), the mass part ratio of the second clear paste to the 1, 3-butanediol is (1-5): (2-9).

When the second clear paste with the relative density and the 1, 3-butanediol are mixed according to the proportion, the optimal effect on keeping the viscosity and the hydrophilicity can be achieved.

In a preferred embodiment, the method for preparing the oil-controlling anti-inflammatory plant combined extract comprises the following steps:

step (1): weighing olive leaves, green leaf gall and glossy privet fruits according to the mass ratio, mixing and crushing, sieving by a 100-mesh sieve, carrying out heating reflux extraction twice by using an ethanol solution with the volume concentration of 65-90%, wherein the first extraction time is 2-4 hours, the second extraction time is 0.5-1.5 hours, and combining filtrates to obtain a first extracting solution;

step (2): weighing traditional Chinese medicine decoction pieces of coptis chinensis, angelica sinensis and liquorice according to a mass ratio, carrying out three-time heating reflux extraction with water, wherein the first extraction time is 1.5-2.5 hours, the second extraction time is 0.5-1.5 hours, and the third extraction time is 0.5 hour, combining filtrates to obtain a second extract, filtering and concentrating until the relative density is 1.10-1.20 measured at 50 ℃, adding absolute ethyl alcohol to enable the alcohol content to reach 48-55%, uniformly stirring, standing for 20-50 hours, and carrying out reduced pressure concentration to obtain a first clear paste with the relative density of 1.35-1.40 measured at 50 ℃;

and (3): mixing the first extract obtained in the step (1) and the first clear paste obtained in the step (2), stirring, standing, filtering, and concentrating under reduced pressure to obtain a second clear paste;

and (4): adding 2-9 parts by mass of 1, 3-butanediol into 1-5 parts by mass of the second fluid extract obtained in the step (3), and uniformly mixing to obtain the oil-control anti-inflammatory plant combined extract.

In another aspect, the present application provides the use of the above oil-controlling anti-inflammatory botanical composition extract, and/or the oil-controlling anti-inflammatory botanical composition extract prepared by the above method, in the preparation of 5 α -reductase inhibitor products, inflammatory factor inhibitor products, acne-suppressing drugs, anti-alopecia products and/or oil-controlling anti-inflammatory skin care products.

Preferably, the 5 alpha-reductase inhibitor product, the inflammatory factor inhibitor product and/or the acne-suppressing drug are in a dosage form including, but not limited to, a topical liniment or cream.

Preferably, the acne comprises acne vulgaris.

Preferably, the 5 α -reductase comprises a type I5 α -reductase and a type II 5 α -reductase.

Preferably, the inflammatory factors include: IL-1 beta, TNF-alpha and COX-2 factors.

Preferably, the dosage forms of the anti-hair loss product and/or the oil-control anti-inflammatory skin care product include, but are not limited to, aqua, cream, lotion, essence and gel.

On the other hand, the plant combination extract provided by the application can also be used for preparing an anti-hair loss product, so that the plant combination extract can be used as a substitute for the existing western anti-hair loss product with higher safety.

In another aspect, the present application further provides an oil-control anti-inflammatory skin care product, which includes the above oil-control anti-inflammatory plant combination extract, and/or the oil-control anti-inflammatory plant combination extract prepared by the above method, and the oil-control anti-inflammatory skin care product includes, by mass: 0.1-10% of the oil-controlling anti-inflammatory plant combined extract.

Preferably, the oil-control anti-inflammatory skin care product further comprises an auxiliary material, wherein the auxiliary material is one or more selected from water, glycerol, 1, 3-butanediol, sodium hyaluronate, betaine, xanthan gum, an emulsifier, an emollient, a humectant and an antioxidant.

In one embodiment, the auxiliary material may be: water, glycerin, 1, 3-butanediol, sodium hyaluronate, betaine, xanthan gum, C14-22 alcohol, C12-20 alkyl glucoside, polydimethylsiloxane/vinyl polydimethylsiloxane crosspolymer, hexanediol, and menthone.

Preferably, the oil-control anti-inflammatory skin care product is essence.

In another aspect, the application provides a preparation method of the oil-control anti-inflammatory skin care product, which comprises the following steps:

step a: dissolving 1, 3-butanediol, glycerol, xanthan gum, sodium hyaluronate and betaine in water, heating to 75-85 ℃, and uniformly stirring to obtain a phase A;

step b: mixing an emulsifier and an emollient, heating to 75-85 ℃, and uniformly stirring to obtain a phase B;

step c: dissolving a humectant and an antioxidant in water to obtain phase C;

step d: mixing the phase B and the phase A, homogenizing at 75-85 ℃ and 3000-4000 r/min for 4-10 min, and cooling at 20-30 r/min at the speed of 1-3 ℃/min; and when the temperature is reduced to 45 ℃, adding the phase C and the oil-control anti-inflammatory plant combined extract, continuously stirring, and cooling to below 40 ℃ to obtain the oil-control anti-inflammatory skin care product.

The following beneficial effects can be brought through the application:

1. the application provides a plant combination extract adopts the component raw materials of specific kind and quantity ratio to mix and extract to learn from the test that it has the instant oil control effect and the long-term oil control effect of obviously promoting, the oil control is effectual fast and difficult relapse, compares and has apparent oil control advantage in common oil control plant extract.

2. The plant combined extract provided by the application can effectively inhibit I-type 5 alpha-reductase and II-type 5 alpha-reductase, and simultaneously inhibit IL-1 beta, TNF-alpha and COX-2 inflammatory factors which are overexpressed due to breeding of anaerobic bacteria acne bacillus in skin hair follicles, on one hand, both symptoms and root causes can be treated, on the other hand, the oil control and anti-inflammatory effects can be exerted together, and when the plant combined extract is used for preparing acne inhibition medicines, anti-alopecia products and/or oil control and anti-inflammatory skin care products, a more remarkable synergistic effect can be shown compared with a mode of mixing single effect components.

3. The plant combination extract provided by the application adopts traditional Chinese medicine plant sources, has the advantages of good naturalness, good skin-friendly property, no toxic or side effect, mildness, no irritation and good use experience.

4. The preparation method of the plant combination extract provided by the application has the advantages that the extraction efficiency is high, the purity is good, the extraction process is pollution-free, the extraction solvent ethanol can be recovered, the extraction is performed twice after multiple times of extraction, the extract is mixed with 1, 3-butanediol in a clear paste form, on one hand, the content of active ingredients in unit mass or volume is improved, on the other hand, the moisture retention property of the 1, 3-butanediol is favorable for promoting permeation, and the hydrophilic active ingredients are assisted to exert effects for a long time.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the application and together with the description serve to explain the application and not to limit the application. In the drawings:

FIG. 1 is a VISIA-CR photograph of the skin care product of example 3 taken at a time point prior to use in a human efficacy experiment;

FIG. 2 is a VISIA-CR photograph of the skin care product of example 3 taken at a time point after 1 week of use in a human efficacy experiment;

FIG. 3 is a VISIA-CR photograph of the skin care product of example 3 taken 4 weeks after use in a human efficacy experiment;

FIG. 4 is a VISIA-CR photograph of the skin care product of example 3 taken at a time point after 1 week of disuse in a human efficacy test;

FIG. 5 is a VISIA-CR photograph of the skin care product of comparative example 1 taken at a time point before use in a human efficacy test;

FIG. 6 is a VISIA-CR photograph of the skin care product of comparative example 1 taken at a time point of 1 week after use in a human efficacy test;

FIG. 7 is a VISIA-CR photograph of the skin care product of comparative example 1 taken 4 weeks after use in a human efficacy experiment;

fig. 8 is a VISIA-CR photograph of the skin care product prepared in comparative example 1 in a human efficacy experiment, taken at a time point after 1 week of disuse.

Detailed Description

In order to more clearly explain the overall concept of the present application, the following detailed description of the overall scheme of the present invention is made by way of example. In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. It will be apparent, however, to one skilled in the art, that the present invention may be practiced without one or more of these specific details. In other instances, well-known features have not been described in order to avoid obscuring the invention.

Unless otherwise specified, the starting components in the examples described below are commercially available, the laboratory instruments used are conventional laboratory instruments, and the test methods are known in the art.

In addition, the "water" in the present invention includes any available water that can be used in the cosmetic field such as deionized water, distilled water, ion-exchanged water, double distilled water, high purity water, purified water, and the like.

Example 1

The embodiment provides an oil-control anti-inflammatory plant combined extract, which is prepared by adopting the following method:

step (1): respectively weighing 40g of olive leaf, 30g of swertia mileensis and 20g of glossy privet fruit, crushing the raw materials into 100 meshes at a high speed, sieving, and uniformly mixing to obtain mixed powder; mixing the mixed powder with an ethanol solution with a volume concentration of 70% according to a material-liquid ratio of 1 g: mixing 10ml of the raw materials, extracting twice, wherein the first extraction adopts heating reflux extraction for 3 hours, filtering to separate filtrate from filter residue, and mixing the filter residue with 70% ethanol solution according to a material-liquid ratio of 1 g: mixing 10ml of the raw materials in a proportion, performing secondary extraction, performing heating reflux extraction for 1 hour, filtering, and combining filtrates obtained by the two extractions to obtain a first extracting solution;

step (2): weighing 5g of coptis decoction pieces, 10g of angelica decoction pieces and 8g of liquorice decoction pieces respectively, extracting with water for three times, wherein the water with the mass 10 times of that of the mixed medicinal material decoction pieces is adopted in the first extraction, heating and refluxing for 2 hours, and filtering; the second extraction adopts water with the mass 5 times of that of the mixed medicinal material decoction pieces, and the heating reflux extraction is carried out for 1 hour and the filtration is carried out; the third extraction adopts water with the mass 5 times of that of the mixed medicinal material decoction pieces, and the heating reflux extraction is carried out for 0.5 hour and the filtration is carried out; combining the filtrates obtained by the three extractions, and concentrating the filtrates until the relative density is 1.10-1.20 (50 ℃) to obtain a second extracting solution;

and (3): adding absolute ethanol into the second extracting solution obtained in the step (2) to enable the content of the ethanol to reach 50%, stirring, standing for 24 hours for alcohol precipitation, filtering, recovering ethanol from filtrate under reduced pressure, and concentrating to obtain a first fluid extract with the relative density of 1.35-1.40 (50 ℃);

and (4): mixing the first extract obtained in the step (1) and the first clear paste obtained in the step (3), uniformly stirring, standing for 24 hours, filtering, recovering ethanol from filtrate under reduced pressure, and concentrating to obtain a second clear paste with the relative density of 1.35-1.45 (50 ℃);

and (5): adding 5 parts by mass of 1, 3-butanediol into each 1 part by mass of the second clear paste prepared in the step (4), and blending and uniformly mixing to obtain the oil-controlling anti-inflammatory plant combined extract. The viscosity was measured using a rotary viscometer model NDJ8S and found to be 1100 mPa.s.

Example 2

The embodiment provides an oil-control anti-inflammatory plant combined extract, which is prepared by adopting the following method:

step (1): respectively weighing 30g of olive leaf, 25g of swertia mileensis and 25g of glossy privet fruit, crushing the raw materials into 100 meshes at a high speed, sieving, and uniformly mixing to obtain mixed powder; mixing the mixed powder with an ethanol solution with a volume concentration of 70% according to a material-liquid ratio of 1 g: mixing 10ml of the raw materials, extracting twice, wherein the first extraction adopts heating reflux extraction for 3 hours, filtering to separate filtrate from filter residue, and mixing the filter residue with 70% ethanol solution according to a material-liquid ratio of 1 g: mixing 10ml of the raw materials in a proportion, performing secondary extraction, performing heating reflux extraction for 1 hour, filtering, and combining filtrates obtained by the two extractions to obtain a first extracting solution;

step (2): weighing 10g of coptis decoction pieces, 5g of angelica decoction pieces and 5g of liquorice decoction pieces respectively, extracting with water for three times, wherein the water with the mass 10 times of that of the mixed medicinal material decoction pieces is adopted in the first extraction, heating and refluxing for 2 hours, and filtering; the second extraction adopts water with the mass 5 times of that of the mixed medicinal material decoction pieces, and the heating reflux extraction is carried out for 1 hour and the filtration is carried out; the third extraction adopts water with the mass 5 times of that of the mixed medicinal material decoction pieces, and the heating reflux extraction is carried out for 0.5 hour and the filtration is carried out; combining the filtrates obtained by the three extractions, and concentrating the filtrates until the relative density is 1.10-1.20 (50 ℃) to obtain a second extracting solution;

and (3): adding absolute ethanol into the second extracting solution obtained in the step (2) to enable the content of the ethanol to reach 50%, stirring, standing for 24 hours for alcohol precipitation, filtering, recovering ethanol from filtrate under reduced pressure, and concentrating to obtain a first fluid extract with the relative density of 1.35-1.40 (50 ℃);

and (4): mixing the first extract obtained in the step (1) and the first clear paste obtained in the step (3), uniformly stirring, standing for 24 hours, filtering, recovering ethanol from filtrate under reduced pressure, and concentrating to obtain a second clear paste with the relative density of 1.35-1.45 (50 ℃);

and (5): adding 6 parts by mass of 1, 3-butanediol into each 1 part by mass of the second clear paste prepared in the step (4), and blending and uniformly mixing to obtain the oil-controlling anti-inflammatory plant combined extract. The viscosity was measured using a rotary viscometer model NDJ8S and found to be 1200 mPa.s.

Example 3

The embodiment provides an oil-control anti-inflammatory plant combined extract, which is prepared by adopting the following method:

step (1): respectively weighing 25g of olive leaf, 20g of swertia mileensis and 40g of privet fruit, crushing the raw materials into 100 meshes at a high speed, sieving, and uniformly mixing to obtain mixed powder; mixing the mixed powder with an ethanol solution with the volume concentration of 80% according to the material-liquid ratio of 1 g: mixing 10ml of the raw materials, extracting twice, wherein the first extraction adopts heating reflux extraction for 3 hours, filtering to separate filtrate from filter residue, and mixing the filter residue with an ethanol solution with the volume concentration of 80% according to the material-liquid ratio of 1 g: mixing 10ml of the raw materials in a proportion, performing secondary extraction, performing heating reflux extraction for 1 hour, filtering, and combining filtrates obtained by the two extractions to obtain a first extracting solution;

step (2): weighing 15g of coptis decoction pieces, 8g of angelica decoction pieces and 10g of liquorice decoction pieces respectively, extracting with water for three times, wherein the water with the mass 10 times of that of the mixed medicinal material decoction pieces is adopted in the first extraction, heating and refluxing for 2 hours, and filtering; the second extraction adopts water with the mass 5 times of that of the mixed medicinal material decoction pieces, and the heating reflux extraction is carried out for 1 hour and the filtration is carried out; the third extraction adopts water with the mass 5 times of that of the mixed medicinal material decoction pieces, and the heating reflux extraction is carried out for 0.5 hour and the filtration is carried out; combining the filtrates obtained by the three extractions, and concentrating the filtrates until the relative density is 1.10-1.20 (50 ℃) to obtain a second extracting solution;

and (3): adding absolute ethanol into the second extracting solution obtained in the step (2) to enable the content of the ethanol to reach 50%, stirring, standing for 24 hours for alcohol precipitation, filtering, recovering ethanol from filtrate under reduced pressure, and concentrating to obtain a first fluid extract with the relative density of 1.35-1.40 (50 ℃);

and (4): mixing the first extract obtained in the step (1) and the first clear paste obtained in the step (3), uniformly stirring, standing for 24 hours, filtering, recovering ethanol from filtrate under reduced pressure, and concentrating to obtain a second clear paste with the relative density of 1.35-1.45 (50 ℃);

and (5): adding 7 parts by mass of 1, 3-butanediol into each 1 part by mass of the second clear paste prepared in the step (4), and blending and uniformly mixing to obtain the oil-controlling anti-inflammatory plant combined extract. The viscosity was measured using a rotary viscometer model NDJ8S and found to be 1400 mPa.s.

Example 4

The embodiment provides an oil-control anti-inflammatory plant combined extract, which is prepared by adopting the following method:

step (1): respectively weighing 20g of olive leaf, 40g of swertia mileensis and 25g of glossy privet fruit, crushing the raw materials into 100 meshes at a high speed, sieving, and uniformly mixing to obtain mixed powder; mixing the mixed powder with an ethanol solution with a volume concentration of 90% according to a material-liquid ratio of 1 g: mixing 10ml of the raw materials, extracting twice, wherein the first extraction adopts heating reflux extraction for 3 hours, filtering to separate filtrate from filter residue, and mixing the filter residue with an ethanol solution with the volume concentration of 90% according to the material-liquid ratio of 1 g: mixing 10ml of the raw materials in a proportion, performing secondary extraction, performing heating reflux extraction for 1 hour, filtering, and combining filtrates obtained by the two extractions to obtain a first extracting solution;

step (2): weighing 20g of coptis decoction pieces, 10g of angelica decoction pieces and 10g of liquorice decoction pieces respectively, extracting with water for three times, wherein the water with the mass 10 times that of the mixed medicinal material decoction pieces is adopted in the first extraction, heating and refluxing for 2 hours, and filtering; the second extraction adopts water with the mass 5 times of that of the mixed medicinal material decoction pieces, and the heating reflux extraction is carried out for 1 hour and the filtration is carried out; the third extraction adopts water with the mass 5 times of that of the mixed medicinal material decoction pieces, and the heating reflux extraction is carried out for 0.5 hour and the filtration is carried out; combining the filtrates obtained by the three extractions, and concentrating the filtrates until the relative density is 1.10-1.20 (50 ℃) to obtain a second extracting solution;

and (3): adding absolute ethanol into the second extracting solution obtained in the step (2) to enable the content of the ethanol to reach 50%, stirring, standing for 24 hours for alcohol precipitation, filtering, recovering ethanol from filtrate under reduced pressure, and concentrating to obtain a first fluid extract with the relative density of 1.35-1.40 (50 ℃);

and (4): mixing the first extract obtained in the step (1) and the first clear paste obtained in the step (3), uniformly stirring, standing for 24 hours, filtering, recovering ethanol from filtrate under reduced pressure, and concentrating to obtain a second clear paste with the relative density of 1.35-1.45 (50 ℃);

and (5): adding 9 parts by mass of 1, 3-butanediol into each 1 part by mass of the second clear paste prepared in the step (4), and blending and uniformly mixing to obtain the oil-controlling anti-inflammatory plant combined extract. The viscosity was measured using a rotary viscometer model NDJ8S and found to be 1700 mPa.s.

Example 5

This example provides a plant combination extract, which was prepared in substantially the same manner as in example 3, except that olive leaves were not included and the amount of ligustrum lucidum was 65g, that is, 25g of ligustrum lucidum was used instead of olive leaves.

Example 6

This example provides a plant combination extract, which was prepared in the same manner as in example 3, except that angelica was not included, and coptis chinensis was used in an amount of 23g, i.e., 8g of coptis chinensis was used instead of angelica.

Example 7

This example provides a plant combination extract, which was prepared in substantially the same manner as in example 3, except that 85g of hawthorn was used instead of olive leaf, privet fruit, and swertia mileensis to prepare the first extract.

Comparative example 1

The comparative example is prepared by compounding commercially available plant extracts with known oil control effects, and specifically comprises 1 part by mass of a witch hazel extract, a calendula extract and an aloe extract, wherein the mass parts of the witch hazel extract, the calendula extract and the aloe extract are 0.3:0.3:0.4, and 7 parts by mass of 1, 3-butanediol is added to the mixture and uniformly mixed.

Comparative example 2

This comparative example provides a plant combination extract, which was prepared in substantially the same manner as in example 3, except that the second extract obtained by three extractions with water in step (2) was directly mixed with the first extract, concentrated, and concentrated to a concentrated solution having a relative density of 1.2 to 1.4(50 ℃), i.e., without the use of two alcohol precipitations, and the viscosity of the obtained extract was 500 mpa.s.

Comparative example 3

This comparative example provides a plant combination extract that was prepared in much the same manner as example 3, except that only the second fluid extract was prepared, the step of mixing 1, 3-butanediol was eliminated, and the viscosity of the resulting extract was measured to be 900 mpa.s.

Efficacy test

Oil control effect test

1. Real-time oil control effect test

The plant combined extracts prepared in the above examples and comparative examples are respectively prepared into samples with mass concentration of 5% by using purified water as a solvent, and the purified water without any extract is used as a blank control group to perform an instant oil control effect test on the arm skin of a skin tester.

The experimental method comprises the following steps: selecting two 4cm × 4cm test areas corresponding to the inner sides of two arms of 25 skin testers, respectively marking A and B, and respectively smearing (1.0 + -0.1) mg/cm in the two areas with latex finger sleeves²Squalane, and after applying for 3 minutes, applying (2.0 + -0.1) mg/cm in area A²The purified water is used as blank control, and is smeared on area B (2.0 + -0.1) mg/cm²5% strength sample. After application of the sample, the sample was prepared using a method supplied by Cortex of DenmarkThe sebum test system of skin detector of skinnCombo in SkinLab uses the probe head to test the experimental area and give the oil light value, and takes the average value (one decimal) of the results of all testers as the final result of the sample. The specific oil light value data for each example is shown in table 1.

TABLE 1

Examples of the invention	Gloss value of oil
		Blank control group	＞99
Example 1	57.2
		Example 2	61.1
Example 3	53.3
		Example 4	63.2
Example 5	72.6
		Example 6	70.4
Example 7	76.2
		Comparative example 1	82.9
Comparative example 2	69.5
		Comparative example 3	65.8

As can be seen from the data in Table 1, the oil gloss value of the blank control group without any extract is still the oil gloss value under only angular squalane, the inner side of the arm feels greasy and not refreshing, while the oil gloss values in the areas of the examples and the comparative examples are reduced to different degrees, compared with the commercially available conventional oil control extract, the extract obtained by replacing other raw materials and the extract obtained by different preparation processes, the examples 1 to 4 have quick response, the oil gloss value reduction amplitude is larger, the inner side of the arm feels refreshing and not greasy, and the instant oil control effect is obviously higher than that of the other examples and the comparative examples, particularly the instant oil control effect of the example 3 is most obvious.

2. Durable oil control effect test

The plant combined extracts prepared in the above examples and comparative examples are respectively prepared into samples with mass concentration of 5% by using purified water as a solvent, and a lasting oil control effect test is performed on forehead skin of a skin tester. Wherein the ones provided by Cortex of Denmark are still usedSkinLabThe sebum test system of the Combo skin detector detects the amount of oil.

The experimental method comprises the following steps: selecting 10 testers, cleaning the testers 'face with a standard facial cleanser in a constant temperature and humidity chamber (temperature 20 ℃ +/-1 ℃ and relative humidity 50% +/-10%), measuring the initial value of the skin oil content after wiping, applying 5% of sample (2.0 +/-0.5) g on the testers' face, uniformly coating, and testing the forehead skin oil content (unit: mu g/cm) at 1h, 3h, 5h, 7h and 12h after coating²). Wherein the blank data is data obtained by detection at different time points when no sample is applied, the pure water data is data obtained by detection at different time points when pure water not containing any extract is used, and the fat reduction rate (two decimal places are reserved) of each sample is calculated by the following formula:

oil and fat reduction rate (amount of oil and fat at this time point in blank group-amount of oil and fat at this time point after use of sample)/amount of oil and fat at this time point in blank group X100%

The specific experimental results and the calculated reduction rate of fats and oils are shown in table 2.

TABLE 2

As can be seen from the data in table 2, there was almost no difference in the forehead skin oil development between the use of pure water and the use of no sample compared to the blank without any sample applied, and comparative example 1 using a commercially available common oil-controlling extract did also have problems of slow onset of action and poor long-lasting oil-controlling effect. The long-acting oil control improvement effect of the plant combined extract is obvious, the oil control can be continued for 12 hours after the plant combined extract is used, and the oil reduction rate reaches the best in 3 hours. In addition, the data of comparative examples 5 to 7 and the data of comparative examples 2 to 3 show that the improvement of the process has a certain auxiliary effect, and the specific selection of the raw materials of the plant combination extract is the factor which has a main influence on the long-acting oil control effect, which indicates that the effect is obtained under the combined action of the extracts of all the raw material components.

In summary, examples 1 to 4 are preferred examples of the present application, and therefore the following experiments were conducted only with the plant combination extracts obtained in examples 1 to 4.

Second, inhibition of 5 alpha-reductase

An in-vitro screening model of the 5 alpha-reductase inhibitor is established by using ultra-high performance liquid chromatography, and the inhibitory activity of the plant combined extract on the 5 alpha-reductase is investigated.

The experimental method comprises the following steps: extracting type I and type II 5 alpha-reductase from SD rats, respectively preparing 5 mass concentration gradients of the plant combined extract of each example as an inhibitor, measuring the change of testosterone concentration before and after reaction by using ultra high performance liquid chromatography with testosterone as a substrate and finasteride as a positive control, and calculating the inhibition rate, wherein the inhibition rate is calculated by the following formula:

inhibition rate ═ change in testosterone concentration in blank tube-change in testosterone concentration in sample tube)/change in testosterone concentration in blank tube × 100%

The specific test results and the calculated inhibition rates are shown in table 3.

TABLE 3

As can be seen from the data in table 3, the plant combination extracts provided in examples 1 to 4 all exhibit inhibitory effects on type i 5 α -reductase and type ii 5 α -reductase, and can be used as a natural and safe substitute drug for finasteride, which is a positive control drug, and particularly the plant combination extract provided in example 3 has a significant effect. And when the concentration is more than 10%, the inhibition effect on the I type 5 alpha-reductase and the II type 5 alpha-reductase is strong, and the inhibition rate is more than 50%.

Third, experiment for inhibiting inflammatory factor

The inhibition of the expression of proinflammatory cytokines IL-1 beta, TNF-alpha and COX-2 was determined by a mouse macrophage respiratory burst assay.

The test method comprises the following steps: phorbol ester is used for stimulating macrophages to generate respiratory burst, active oxygen generated by the respiratory burst is detected by chemiluminescence analysis and electron paramagnetic resonance, the expressions of IL-1 beta, TNF-alpha and COX-2 factors are detected, the inhibition rates of three proinflammatory factors under low, medium and high concentrations in each embodiment are calculated, and the obtained results are shown in tables 4 and 5.

TABLE 4 comparison of respiratory bursts and IL-1 beta, TNF-alpha, COX-2 factor expression in mouse macrophages

Note: comparing with normal group, wherein P is less than 0.05; compared with model group, P < 0.01

TABLE 5

The data in tables 4 and 5 show that the plant composition extracts obtained in examples 1 to 4 can inhibit type i hypersensitivity by inhibiting IL-1 β factor, inhibit type i hypersensitivity (immediate hypersensitivity) by inhibiting TNF- α factor, and inhibit inflammation by inhibiting COX-2 factor expression. In addition, the problem of oily skin inflammation caused by the breeding of the anaerobic bacteria acne bacillus can be prevented, cured and improved by inhibiting the three inflammation-promoting factors.

Application examples

The embodiment provides an essence skin care product with oil control and anti-inflammatory effects, which takes the plant combination extract provided by the application as an effective component. Wherein, the components and the dosage of the skin care product are shown in the table 6 according to the mass percentage.

TABLE 6

The specific preparation method of the oil-control anti-inflammatory skin care product comprises the following steps:

step a: dissolving 1, 3-butanediol, glycerol, xanthan gum, sodium hyaluronate and betaine in water according to the mass ratio, heating to 80 ℃, and uniformly stirring to obtain a phase A;

step b: mixing ML, DC200(100 viscosity) and EL-61 according to the mass portion ratio, heating to 80 ℃ and uniformly stirring to obtain a phase B;

step c: dissolving hexanediol and menthone in water according to the mass part ratio, and separately holding the hexanediol and the menthone in a clean vessel at the temperature of below 60 ℃ for later use to obtain a phase C;

step d: mixing phase B and phase A, homogenizing at 80 deg.C and 3500r/min for 5min, and cooling at 25r/min at 2 deg.C/min; and when the temperature is reduced to 45 ℃, adding the C phase and the oil-control anti-inflammatory plant combined extract, continuously stirring, and cooling to below 40 ℃ to obtain the oil-control anti-inflammatory skin care product.

The plant combined extracts of the preferable embodiments 1 to 4 and the comparative examples 1 to 3 are prepared into the skin care essence according to the method, and auxiliary materials of the skin care essence do not contain substances which are easy to stimulate, such as traditional preservatives and the like, so that the skin care essence is better in nature and skin feel.

The essence prepared in the embodiments 1-4 is good in texture and strong in use experience, and the essences prepared in the comparative examples 2 and 3 are very thin in texture and poor in use. The viscosity of the plant combined extract obtained in examples 1 to 4 is greater than 1000mpa.s, so that the essence can be prepared by adopting the above proportion, while the extract prepared in comparative examples 2 and 3 is thin, particularly the viscosity of comparative example 2 is only 500mpa.s, so that the extract is not easy to thicken under the condition of adopting the same mass concentration, cannot be prepared into an essence dosage form, is not beneficial to formulation application, and is abandoned. Therefore, the preparation method provided by the application plays a certain role in assisting the efficacy of the active ingredients and improving the application suitability of the active ingredients.

Fourth, evaluation of human body efficacy

The test method comprises the following steps: healthy men and women with oily skin, ordinary acne between 18 years old and 40 years old and meeting I, II-grade standard are selected as objects, volunteers meeting conditions are recruited, oil-control anti-inflammatory essence products prepared in example 3 and the commercially available comparative example 1 with the best effect measured in a laboratory are respectively used, and various index detections are completed through a facial image analyzer VISIA-CR to evaluate the oil-control anti-inflammatory effect of the products, so that the inflammation problem caused by the breeding of the anaerobic bacterium acnes is treated.

The specific test flow is as follows:

(1) qualified volunteers entered the official experiment. The face was cleaned prior to use and after 15 minutes basal skin condition tests were performed, including lipid, VISIA-CR photographs.

(2) The test sample was taken, the face was cleaned in the morning and evening each day, and the product was used after a toner. Note the situation that occurs during use. If not, the patient can be immediately stopped.

(3) Data were collected before, after 1 week, 4 weeks and after 1 week of disuse of the product, and the oil-controlling anti-inflammatory effect of the product was evaluated on the basis of the area of the red zone in the image taken by VISIA-CR (see left 3 rd picture of the second row photograph in each figure).

The results of two selected of the above volunteers, using individuals with a very close frontal red zone, are shown in the figure for comparison. Wherein, the test results of the volunteers using the skin care product prepared in the example 3 are shown in fig. 1-4, and the test results of the volunteers using the skin care product prepared in the comparative example 1 are shown in fig. 5-8.

As can be seen from the results shown in fig. 1 to 4, the red area of the volunteer who used the skin care product prepared in example 3 before use was 87%, decreased to 80% after one week of use, decreased to 67% after four weeks of use, and the total decrease reached 20%, it was found that the oil-controlling anti-inflammatory effect was very significant, and after one week of non-use, the red area was only 79%, which is not much different from the effect of one week of use, indicating that it has a long-lasting effect in practical use and is not easy to repeat.

From the results of fig. 5 to 8, it can be seen that the volunteers who used the skin care product prepared in comparative example 1 had a red zone area of 87% before use, decreased to 78% after one week of use, decreased to 77% after four weeks of use, and had a total decrease of only 10%, and had a red zone area of 86% after one week of rest. It can be seen that the conventional oil-controlling plant extracts such as hamamelis, calendula, aloe, etc. have significantly lower effects than those of the present application and are easily repeated, although they also have certain oil-controlling anti-inflammatory effects.

In summary, the plant combination extract provided by the application adopts a traditional Chinese medicine plant source, and has the advantages of good naturality, good skin-friendly property, no toxic or side effect, mildness, no irritation and good use experience. And laboratory tests and human body efficacy tests show that the oil control agent has an obviously improved instant oil control effect and a long-acting oil control effect, can also effectively inhibit I-type 5 alpha-reductase and II-type 5 alpha-reductase, and simultaneously inhibit over-expressed IL-1 beta, TNF-alpha and COX-2 inflammatory factors caused by breeding of anaerobic bacteria acnes in skin hair follicles, realizes treatment of both symptoms and root causes, has quick and difficult repeated oil control effect, and can be applied to preparation of acne inhibition drugs, anti-alopecia products and/or oil control anti-inflammatory skin care products.

The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.