阅读说明：本技术 一种植物抑菌口腔护理用品 (Plant antibacterial oral care product ) 是由 黄柳云 李江平 胡永志 李秋芳 韦玉金 梁莹莹 刘文佳 于 2020-12-30 设计创作，主要内容包括：本发明公开了一种植物抑菌口腔护理用品,以植物复方抑菌组合物为活性成分,所述植物复方抑菌组合物由牡丹皮提取物、两面针提取物、甘草提取物、葛根提取物、金银花提取物、断血流提取物、三七提取物、升麻提取物组成。本发明是性能安全、稳定有效的抑菌口腔护理产品,其在发挥抑制口腔有害菌的同时,不产生耐药性,能降低化学剂用量及刺激性,达到保障口腔清洁卫生健康、维护口腔微生态平衡的目的,符合满足广大人民群众日常的迫切需求。(The invention discloses a plant bacteriostatic oral care product which takes a plant compound bacteriostatic composition as an active ingredient, wherein the plant compound bacteriostatic composition consists of a tree peony bark extract, a radix zanthoxyli extract, a liquorice extract, a kudzuvine root extract, a honeysuckle extract, a clinopodium polycephalum extract, a pseudo-ginseng extract and a cimicifuga foetida extract. The bacteriostatic oral care product has safe, stable and effective performance, does not generate drug resistance while inhibiting harmful bacteria in the oral cavity, can reduce the dosage and the irritation of chemical agents, achieves the aims of ensuring the cleanness, the sanitation and the health of the oral cavity and maintaining the micro-ecological balance of the oral cavity, and meets the daily urgent requirements of the masses.)

1. A plant antibacterial oral care product is characterized in that:

the plant compound bacteriostatic composition is used as an active ingredient and comprises the following components in parts by weight: 10-20 parts of tree peony bark extract, 1-5 parts of radix zanthoxyli extract, 3-7 parts of liquorice extract, 2-7 parts of kudzu root extract, 3-10 parts of honeysuckle extract, 1-6 parts of clinopodium polycephalum extract, 2-5 parts of pseudo-ginseng extract and 2-6 parts of cimicifuga foetida extract.

2. The plant bacteriostatic oral care product of claim 1, wherein:

the weight percentage of the plant compound antibacterial composition in the oral care product is 0.1-10.0%.

3. The plant bacteriostatic oral care product of claim 1, wherein:

the weight percentage of the plant compound antibacterial composition in the oral care product is 0.2-3.0%.

4. The plant bacteriostatic oral care product of claim 1, wherein:

the cortex moutan extract is paeonol with a content of more than 90% separated by purification, cooling and crystallization after cortex moutan original drug is extracted by steam distillation.

5. The plant bacteriostatic oral care product of claim 1, wherein:

the radix zanthoxyli extract, the liquorice extract, the radix puerariae extract, the honeysuckle extract, the clinopodium polycephalum extract, the pseudo-ginseng extract and the cimicifuga foetida extract are all ethanol extracts.

6. The plant bacteriostatic oral care product of claim 4, which is characterized in that:

the radix zanthoxyli extract, the liquorice extract, the kudzu root extract, the honeysuckle extract, the clinopodium polycephalum extract, the pseudo-ginseng extract and the cimicifuga foetida extract are extracted and concentrated by ethanol to obtain the corresponding extracts with the mass of 0.2-1.0 time of the mass of the original medicine.

7. The plant bacteriostatic oral care product of claim 5, wherein:

the radix zanthoxyli extract, the liquorice extract, the radix puerariae extract, the honeysuckle extract, the clinopodium polycephalum extract, the pseudo-ginseng extract and the rhizoma cimicifugae extract are extracted and concentrated by ethanol to obtain the corresponding extracts with the mass being 0.5 times of the mass of the original medicine.

8. Use of the plant bacteriostatic oral care product of any one of claims 1 to 6 for inhibiting harmful bacteria in the oral cavity.

9. Use according to claim 7, characterized in that: the oral cavity harmful bacteria are Porphyromonas gingivalis, Streptococcus mutans, Candida albicans, Escherichia coli and Staphylococcus aureus.

Technical Field

The invention relates to the technical field of oral care products, in particular to an oral care product containing plant compound antibacterial ingredients.

Background

According to statistics, the oral cavity prevalence rate of people in China reaches more than 80%, people of all ages are threatened, and oral cavity nursing is urgent. Common oral care includes caries, periodontitis, sore surfaces and other oral disease care, and the infection of the oral disease is closely related to oral flora. Wherein, the main pathogenic bacteria of the dental caries is streptococcus mutans, which is the main cause of the dental caries; the main pathogenic bacteria of periodontitis is Porphyromonas gingivalis, which can be used as endogenous pathogenic bacteria to overgrow under specific conditions to cause periodontal destruction, and is also a main pathogenic anaerobe of halitosis, even a main reason for adult tooth loss; the main pathogenic bacteria of the sore surface are candida albicans, commonly called thrush, and the candida albicans are the most common concurrent infection during HIV infection and mainly cause hairy leukoplakia. At present, the development trend of oral care in China advocates green, environmental protection, safety and pursuit of efficacy. The Chinese herbal medicine has rich sources, definite curative effect of the ancient prescription, small toxic and side effect and high acceptance among common people. Therefore, the concept of plant efficacy has become a hot spot in the toothpaste industry in China.

Disclosure of Invention

The invention discloses a plant bacteriostatic oral care product, which is a bacteriostatic oral care product with safe, stable and effective performance and containing a plant compound bacteriostatic composition, does not generate drug resistance while exerting the function of inhibiting harmful bacteria in an oral cavity, can reduce the dosage and the irritation of a chemical agent, achieves the aims of ensuring the cleanness, the sanitation and the health of the oral cavity and maintaining the micro-ecological balance of the oral cavity, and meets the daily urgent requirements of the masses.

In order to achieve the purpose, the technical scheme of the invention is as follows:

a plant bacteriostatic oral care product takes a plant compound bacteriostatic composition as an active ingredient, wherein the plant compound bacteriostatic composition is prepared from the following components in parts by weight: 10-20 parts of tree peony bark extract, 1-5 parts of radix zanthoxyli extract, 3-7 parts of liquorice extract, 2-7 parts of kudzu root extract, 3-10 parts of honeysuckle extract, 1-6 parts of clinopodium polycephalum extract, 2-5 parts of pseudo-ginseng extract and 2-6 parts of cimicifuga foetida extract.

Preferably, the weight percentage of the plant compound antibacterial composition in the oral care product is 0.1-10.0%. More preferably, the weight percentage of the plant compound antibacterial composition in the oral care product is 0.2-3.0%.

Preferably, the moutan bark extract is a paeonol component with the content of more than 90% separated by purification, cooling and crystallization after the moutan bark original drug is extracted by steam distillation.

Preferably, the radix zanthoxyli extract, the licorice extract, the radix puerariae extract, the honeysuckle extract, the clinopodium polycephalum extract, the panax notoginseng extract and the cimicifuga foetida extract are all ethanol extracts.

Preferably, the radix zanthoxyli extract, the licorice extract, the radix puerariae extract, the honeysuckle extract, the clinopodium polycephalum extract, the pseudo-ginseng extract and the cimicifuga foetida extract are extracted and concentrated by ethanol to obtain the corresponding extracts with the mass of 0.2-1.0 time of the mass of the original medicine. More preferably, the radix zanthoxyli extract, the liquorice extract, the radix puerariae extract, the honeysuckle extract, the clinopodium polycephalum extract, the pseudo-ginseng extract and the rhizoma cimicifugae extract are extracted and concentrated by ethanol to obtain the corresponding extract with the mass of 0.5 time of the mass of the original medicine.

The invention also provides application of the plant bacteriostatic oral care product in inhibiting harmful bacteria in the oral cavity.

The oral care product comprises toothpaste, mouthwash, tooth powder, chewing gum and the like.

Further, the oral cavity harmful bacteria are Porphyromonas gingivalis, Streptococcus mutans, Candida albicans, Escherichia coli and Staphylococcus aureus.

The plant antibacterial oral care product adopts the following plant components:

cortex moutan, a dried root bark of Paeonia suffruticosa Andr of Ranunculaceae, has effects of clearing heat and cooling blood, promoting blood circulation to remove blood stasis, and relieving deficiency heat. The main components comprise paeonol, paeoniflorin and the like, wherein the paeonol is used as the main drug effective component and has various pharmacological effects of reducing blood pressure, stopping bleeding and the like.

Radix Zanthoxyli is the dried root of radix Zanthoxyli of Rutaceae, has effects of promoting blood circulation for removing blood stasis, activating qi-flowing and relieving pain, and can be used for treating traumatic injury, stomach ache, toothache, rheumatalgia, and venomous snake bite; it can be used for external treatment of burn and scald.

Glycyrrhrizae radix, which is the dried root and rhizome of Glycyrrhiza uralensis Fisch of Papilionaceae of Leguminosae, has antiinflammatory and antiallergic effects, and can protect inflamed throat and trachea mucosa.

Radix Puerariae is dried root of Pueraria lobata Ohwi of Leguminosae, and has effects of relieving muscles and fever, promoting eruption, promoting fluid production to quench thirst, invigorating yang and relieving diarrhea.

Flos Lonicerae, the bud of Lonicera Japonica flos of Caprifoliaceae, has effects of clearing away heat and toxic materials, relieving inflammation and detumescence, and can be used for treating bacillary dysentery and various suppurative diseases.

Clinopodium polycephalum, which is the dry aerial part of downy groundsel herb or ledebouriella seseloides bunge of Labiatae, has astringent and hemostatic effects, and can be used for treating metrorrhagia, hematuria, epistaxis, gingival hemorrhage, and traumatic hemorrhage.

Notoginseng radix, a dry root of Panax notoginseng (Burk.) F.H. Chen of Araliaceae, has hemostatic, blood stasis dispelling, antiinflammatory, analgesic and tonic effects.

Cimicifugae rhizoma, is rhizome of Cimicifuga racemosa, Cimicifuga dahurica or Cimicifuga foetida of genus Cimicifuga of family Ranunculaceae, and has effects of relieving exterior syndrome, promoting eruption, clearing away heat and toxic materials, and invigorating yang and lifting sink, and can be used for treating laryngalgia, aphtha, and macula obstruction.

The plant compound bacteriostatic composition can effectively inhibit oral harmful bacteria such as porphyromonas gingivalis, streptococcus mutans, candida albicans, escherichia coli, staphylococcus aureus and the like under the synergistic effect of various plant components, and tests prove that when the plant compound bacteriostatic composition is applied to toothpaste, the bacteriostatic rate of staphylococcus aureus and escherichia coli in aerobic bacteria can reach 99.99 percent, the bacteriostatic rate of candida albicans can reach 74.31 percent, the plant compound bacteriostatic composition has an obvious inhibiting effect on a biological cell growth curve of porphyromonas gingivalis in anaerobic bacteria, has a certain inhibiting effect on a biological cell growth curve of streptococcus mutans, and can reduce the propagation rate and the growth time; the plant compound bacteriostatic composition is applied to mouthwash, has the bacteriostatic rate of 99.99 percent on staphylococcus aureus and escherichia coli in aerobic bacteria and 99.90 percent on candida albicans, and also has obvious inhibitory effect on biological cell growth curves of porphyromonas gingivalis and streptococcus mutans in anaerobic bacteria. The aim of preventing and treating decayed teeth can be achieved by inhibiting harmful bacteria in the oral cavity, thereby ensuring the cleanness, sanitation and health of the oral cavity.

The invention takes plants as effective components, does not generate drug resistance, can reduce the dosage and the irritation of chemical agents, and achieves the aim of maintaining the micro-ecological balance of the oral cavity.

Drawings

FIG. 1 is a graph showing the growth of Porphyromonas gingivalis in a bacteriostatic test.

FIG. 2 is a graph showing the growth in the Streptococcus mutans bacteriostasis test.

Detailed Description

The present invention is further illustrated by the following specific examples, but the scope of the present invention is not limited to the following examples.

The moutan bark extract adopted in this example is a crude moutan bark drug, which is subjected to steam distillation extraction, and then is subjected to suction filtration to obtain a crude crystal, and then the crude crystal is purified according to the proportion of the crude crystal comprising 95% ethanol and purified water =1:4:15, and is cooled and kept stand at 0 ℃ for 48 hours, so that a needle-shaped crystal with a content of paeonol larger than 90% is separated out.

The radix zanthoxyli extract, the liquorice extract, the radix puerariae extract, the honeysuckle extract, the clinopodium polycephalum extract, the pseudo-ginseng extract and the rhizoma cimicifugae extract are respectively extracted by reflux according to the proportion of 75vt percent of ethanol =1:3 of a raw medicine, and the extract is concentrated to 1/2 of the mass of the raw medicine.

Firstly, zone of inhibition test of the plant compound bacteriostatic composition:

1.1 test grouping:

the zone of inhibition test was divided into 5 groups:

group A: moutan cortex Radicis extract. Group B: radix zanthoxyli, honeysuckle and pseudo-ginseng in a weight ratio of 2:3: 5. Group C: the liquorice, the kudzuvine root, the clinopodium polycephalum and the rhizoma cimicifugae are mixed according to the weight ratio of 3:2:2: 3. Group D (complete group): the components and the corresponding parts by weight are respectively as follows: 20 parts of tree peony bark, 2 parts of radix zanthoxyli, 3 parts of liquorice, 2 parts of kudzu root, 3 parts of honeysuckle, 2 parts of clinopodium polycephalum, 5 parts of pseudo-ginseng and 3 parts of cimicifuga foetida. Group E: blank control group.

1.2 zone of inhibition test protocol

Preparation of bacterial suspension: the slant culture of the test bacteria for 18-24 h is washed with PBS to prepare a bacterial suspension (100. mu.L is dripped into 5 mL of sample liquid, and the number of recovered bacteria is (5 × 105) to (5 × 106) CFU/mL).

The oxford cup method is adopted. And sucking 100 mu L of the bacterial suspension to the surface of the completely solidified nutrient agar culture medium by using a micro liquid transfer gun, immediately and uniformly coating by using a sterile glass coating rod, covering a plate, and drying at room temperature for 5-10 min. Then, the sterile oxford cup is stood on the surface of the culture medium by using sterile forceps, and the culture medium is not broken. And finally, respectively dropwise adding the extracts of all groups to the liquid level to be parallel to the surface of the Oxford cup, placing the culture dish in a 37 ℃ incubator, and observing the result after culturing for 16-18 hours. The size of the inhibition zone is determined by adopting a cross method. According to the judgment standard of the antibacterial zone result of disinfection technical specification and traditional Chinese medicine pharmacology, the method comprises the following steps: the diameter of the inhibition zone is more than or equal to 20mm, the sensitivity is high, the sensitivity is moderate, the sensitivity is low, the sensitivity is less than or equal to 10 mm, and the inhibition effect is not high.

1.3 test results

Results of zone of inhibition test of group 15 extracts

A, B, C, D, E5 groups are adopted to carry out bacteriostatic circle experiments, and it can be found that A, B, C3 groups all have certain bacteriostatic effects, and E group has no bacteriostatic effect; a, B, C3 groups are compounded together, and the obtained group D is subjected to bacteriostasis circle experiments to find that 8 traditional Chinese medicine extracts play a role in synergy, and the diameters of the bacteriostasis circles of staphylococcus aureus, candida albicans and streptococcus mutans are all larger than 20 mm. Therefore, the sensitivity of the bacteriostatic action is from large to small: group D > group A > group B > group C > group E.

Second, antibacterial test of applying plant compound antibacterial composition to oral care product

2.1 application of plant compound antibacterial composition in mouthwash

The mouthwash in examples 1-3 comprises the following raw materials in percentage by mass as shown in table 2:

table 2 mouthwash formulations tables for examples 1-3

2.2 application of plant compound antibacterial composition in toothpaste

The toothpaste in examples 4-6 comprises the following raw materials in percentage by mass as shown in Table 3:

table 3 mouthwash formulations tables for examples 4-6

2.3 bacteriostatic test

(1) Aerobic bacteria inhibition experiment: and (4) carrying out a bacteriostatic effect test according to QBT 2738-2012 evaluation method of antibacterial and bacteriostatic effects of daily chemical products. Under the condition of 20-21 ℃, the experimental concentration of the mouthwash is the stock solution, and the effect is carried out for 2 min; the experimental concentration of the toothpaste is 1:1, the action time is 2min, and the experimental strains are 3 strains of staphylococcus aureus (ATCC 6538), escherichia coli (ATCC 25922) and candida albicans (ATCC 10231); when the bacteriostasis rate is more than or equal to 50 percent, marking that the bacteriostasis effect is achieved, and repeating for three times; when the bacteriostasis rate is more than or equal to 90 percent, the mark shows that the bacteriostatic effect is stronger, and the process is repeated for three times.

(2) Anaerobic bacteria bacteriostasis experiment: and (4) carrying out bacteriostatic effect test by adopting a biological growth curve monitoring method. Preparing a bacteria culture solution by using effective content values of plant compound antibacterial ingredients in mouthwash and toothpaste, and taking the bacteria culture solution as a test group; the method comprises the steps of taking a bacterial culture solution without plant compound bacteriostatic components as a control group, measuring the OD value once by using an ultraviolet spectrophotometer at 35-37 ℃ under the anaerobic condition at intervals of 1 hour, and representing the concentration and growth condition of bacteria. Wherein the measurement wavelength of Porphyromonas gingivalis (ATCC 33277) is 450nm, and the measurement wavelength of Streptococcus mutans (ATCC 25175) is 600 nm. The biological growth curve of the sample group is lower than that of the control group, and the mark shows that the biological growth curve of the sample group has an inhibition effect on the growth of bacteria at the concentration, and has an antibacterial effect.

(3) The experiments of examples 1 and 4 are carried out, and the blank control sample does not contain the plant compound bacteriostatic composition.

2.4 test results

(1) The mouthwash containing the plant compound antibacterial composition has an antibacterial effect; the bacteriostatic mouth wash has the bacteriostatic rate of more than 90 percent on staphylococcus aureus and the bacteriostatic rate of more than 90 percent on escherichia coli, and has stronger bacteriostatic effect; the bacteriostatic rate of the product on Candida albicans is greater than 70%, and the product has bacteriostatic effect.

(2) The toothpaste containing the plant compound antibacterial composition has antibacterial effect; the bacteriostatic mouth wash has the bacteriostatic rate of more than 90 percent on staphylococcus aureus, the bacteriostatic rate of more than 90 percent on escherichia coli and the bacteriostatic rate of more than 90 percent on candida albicans, and has strong bacteriostatic effect.

(3) In the embodiment 1, the contained effective content value of the plant compound bacteriostatic component has obvious inhibition effect on the biological cell growth curve of the porphyromonas gingivalis; has inhibiting effect on the growth curve of biological cells of streptococcus mutans, and can reduce the reproduction rate and the growth time of streptococcus mutans.

(4) In example 4, the effective content of the additional plant compound bacteriostatic components has obvious inhibitory effect on the growth curve of biological cells of porphyromonas gingivalis and streptococcus mutans.

(5) The results of the aerobic bacteria inhibition test for mouthwash are shown in tables 4 and 5:

table 4 example 1 results of aerobic bacteria inhibition test

Table 5 example 4 aerobic bacteria inhibition test

(6) The results of the anaerobe biological growth curve test are shown in table 6 and fig. 1 and 2:

table 6 results of bacteriostatic tests on anaerobic organisms of examples 1 and 4