阅读说明：本技术 用于监测样品中激酶活性的方法 (Method for monitoring kinase activity in a sample ) 是由 阿德里安斯·弗雷德里克·马尔滕·奥尔特拉尔 蒂埃里·托马斯·施米德林 于 2019-06-21 设计创作，主要内容包括：本发明涉及监测样品中激酶活性或活化的方法,该方法包括以下步骤：a)提供包含激酶的样品,b)将样品与蛋白酶一起温育以将步骤a)中提供的激酶裂解为蛋白酶特异性蛋白水解肽,c)对样品应用磷酸肽富集,d)通过液相色谱-质谱法分析步骤c)中获得的样品,以及e)检测步骤a)中提供的激酶的磷酸化,其中,仅在鉴定出与激酶活化区域相关的蛋白水解肽的情况下才执行步骤e)的检测。(The present invention relates to a method of monitoring kinase activity or activation in a sample, the method comprising the steps of: a) providing a sample comprising a kinase, b) incubating the sample with a protease to cleave the kinase provided in step a) into protease-specific proteolytic peptides, c) applying phosphopeptide enrichment to the sample, d) analyzing the sample obtained in step c) by liquid chromatography-mass spectrometry, and e) detecting phosphorylation of the kinase provided in step a), wherein the detection of step e) is performed only if a proteolytic peptide associated with the kinase activation region is identified.)