阅读说明：本技术 一种猪饲料用益生菌添加剂的制备方法 (Preparation method of probiotic additive for pig feed ) 是由 雷江文 于 2020-12-11 设计创作，主要内容包括：本发明属于益生菌技术领域,具体涉及一种猪饲料用益生菌添加剂的制备方法。本发明以新鲜猪肠作为基础原料,通过营养液对新鲜猪肠内部原生态微生物群进行培养,以获得其中优势的菌种,提高菌剂在肠道中的定植性,同时增加了有助于增加家猪体内优势的混合菌剂,随后通过膳食纤维制备得到多孔微球结构,通过多孔微球结构对辅助料吸附,通过微生物吸附在微球表面,利用辅助料对微生物进行提供营养物质,保证微生物稳定生存,而且通过阿拉伯胶进行包裹,同时膳食纤维的存在,可以促进肠道蠕动,形成可以有效解决猪饲料中的活性益生菌在肠道中的定植性能较弱,无法增加家猪的抗病性能,无法对猪进行调理的问题的猪饲料用益生菌添加剂。(The invention belongs to the technical field of probiotics, and particularly relates to a preparation method of a probiotic additive for pig feed. The invention takes the fresh pig intestines as the basic raw material, and the original ecological microbiota in the fresh pig intestines are cultured by the nutrient solution to obtain the dominant strains, improve the planting property of the microbial inoculum in the intestinal tracts, meanwhile, a mixed microbial inoculum which is helpful for increasing the advantages in the domestic pigs is added, and then a porous microsphere structure is prepared by dietary fibers, the auxiliary material is adsorbed by the porous microsphere structure, the microorganism is adsorbed on the surface of the microsphere, the auxiliary material is utilized to provide nutrient substances for the microorganism, the stable survival of the microorganism is ensured, and the gum arabic is used for wrapping, and the existence of dietary fiber can promote intestinal peristalsis, so that the probiotic additive for the pig feed can be used for effectively solving the problems that the colonization performance of active probiotics in the pig feed in the intestinal tract is weak, the disease resistance of domestic pigs cannot be increased, and the pigs cannot be conditioned.)

1. A preparation method of a probiotic additive for pig feed is characterized by comprising the following steps:

(1) putting fresh pork intestines and the nutrient solution into a grinder for grinding according to the mass ratio of 1: 10-15, adjusting the pH value to 6-7, collecting ground materials, standing the ground materials at 25-30 ℃, filtering, collecting filtrate, and uniformly mixing the filtrate and the mixed microbial inoculum according to the mass ratio of 15: 1-3 to obtain base solution;

(2) taking 80-90 parts by weight of peptone, 45-50 parts by weight of agar, 15-25 parts by weight of water, 5-10 parts by weight of glucose and 3-12 parts by weight of additives, uniformly stirring, sterilizing and disinfecting to obtain a solid nutrient, and uniformly mixing the solid nutrient and a base solution according to a mass ratio of 18: 7-9 to obtain a culture;

(3) placing the culture at 28-35 ℃ and the air humidity of 60-65%, culturing for 1-2 days, then leaching the surface of the culture by using distilled water until no hyphae exist on the surface, and collecting leacheate;

(4) uniformly mixing dietary fiber, calcium carbonate and water according to the mass ratio of 3:2: 3-5, adding acetone with the same volume as water, adding a surfactant accounting for 20-30% of the mass of the calcium carbonate, performing ultrasonic oscillation, adding liquid paraffin accounting for 80-85% of the volume of the water, stirring, heating to 30 ℃, preserving heat, filtering, washing, drying, and collecting a dried substance;

(5) and (3) soaking the dried substance in a hydrochloric acid solution, filtering, washing and drying to obtain basic particles, uniformly stirring the basic particles, the auxiliary material, the Arabic gum and the leacheate according to the mass ratio of 10:3:5: 20-25, standing, and freeze-drying to obtain the probiotic additive for the pig feed.

2. The preparation method of the probiotic additive for the pig feed according to claim 1, wherein the nutrient solution in the step (1) is prepared by uniformly stirring 230-330 parts by weight of water, 50-60 parts by weight of peptone, 20-30 parts by weight of yeast extract, 10-13 parts by weight of diammonium citrate, 8-12 parts by weight of glucose and 6-9 parts by weight of sodium chloride, and sterilizing and disinfecting the mixture to obtain the nutrient solution.

3. The preparation method of the probiotic additive for pig feed according to claim 1, wherein the mixed microbial inoculum in the step (1) is prepared by mixing bacillus subtilis and lactobacillus according to any mass ratio.

4. The preparation method of the probiotic additive for pig feed according to claim 1, wherein the additive in the step (2) is prepared by mixing disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium chloride according to a mass ratio of 6:5: 1-3.

5. The preparation method of the probiotic additive for pig feed according to claim 1, wherein the surfactant in the step (4) is a mixture of sodium stearate and lecithin in any mass ratio.

6. The preparation method of the probiotic additive for pig feed according to claim 1, wherein the auxiliary material in the step (5) is formed by mixing peptone and glucose according to a mass ratio of 10: 1.

Technical Field

The invention belongs to the technical field of probiotics, and particularly relates to a preparation method of a probiotic additive for pig feed.

Background

Pork is a daily consumption product of residents, and the safety of the meat quality of the pork is greatly dependent on the safety condition of the pig feed. Although the addition of antibiotics into the pig feed can obviously improve the production performance of pigs and the utilization rate of nutrient substances, the problems of antibiotic residue, environmental pollution and the like of pork products can be caused. Therefore, the pig feed or the additive with the functions of preventing diseases and promoting growth and the like without pollution and residue is developed to replace antibiotics, and has important practical significance for improving the pork quality safety and the like.

The probiotics are beneficial microorganisms which have great promotion effect on the health of the host, and are beneficial to the balance of the host intestinal microorganisms. In recent years, extensive practical studies prove that the probiotics play an important role in maintaining intestinal balance, improving animal health and promoting animal growth. It is currently widely accepted in the field of aquaculture that probiotics are the best alternative to antibiotics. Experiments prove that the growth performance and the feed utilization efficiency of piglets and fattening pigs can be improved by directly adding probiotics into the pig feed, and the occurrence of various diseases is effectively reduced.

In the production process of the existing microecological feed additive for pigs, most of the probiotics are only protein substances and/or carbohydrate substances serving as culture media of the active probiotics, although the number of strains can meet the requirements, the activity of most of the strains is not high, and the colonization performance in intestinal tracts is weak, so that the function of the active probiotics serving as the livestock feed is greatly limited; meanwhile, the active probiotics can only enhance the physique of the domestic pig to a certain extent, cannot enhance the disease prevention and resistance of the domestic pig, does not have the function of disease treatment, and cannot condition the pig, so that the existing domestic pig micro-ecological feed additive cannot completely replace antibiotics.

Disclosure of Invention

The technical problems to be solved by the invention are as follows: aiming at the problems that the colonization performance of active probiotics in pig feed in intestinal tract is weak, the disease resistance of domestic pigs cannot be increased, and the pigs cannot be conditioned, the preparation method of the probiotic additive for the pig feed is provided.

In order to solve the technical problems, the invention adopts the following technical scheme:

a preparation method of a probiotic additive for pig feed comprises the following steps:

(1) putting fresh pork intestines and the nutrient solution into a grinder for grinding according to the mass ratio of 1: 10-15, adjusting the pH value to 6-7, collecting ground materials, standing the ground materials at 25-30 ℃, filtering, collecting filtrate, and uniformly mixing the filtrate and the mixed microbial inoculum according to the mass ratio of 15: 1-3 to obtain base solution;

(2) taking 80-90 parts by weight of peptone, 45-50 parts by weight of agar, 15-25 parts by weight of water, 5-10 parts by weight of glucose and 3-12 parts by weight of additives, uniformly stirring, sterilizing and disinfecting to obtain a solid nutrient, and uniformly mixing the solid nutrient and a base solution according to a mass ratio of 18: 7-9 to obtain a culture;

(3) placing the culture at 28-35 ℃ and the air humidity of 60-65%, culturing for 1-2 days, then leaching the surface of the culture by using distilled water until no hyphae exist on the surface, and collecting leacheate;

(4) uniformly mixing dietary fiber, calcium carbonate and water according to the mass ratio of 3:2: 3-5, adding acetone with the same volume as water, adding a surfactant accounting for 20-30% of the mass of the calcium carbonate, performing ultrasonic oscillation, adding liquid paraffin accounting for 80-85% of the volume of the water, stirring, heating to 30 ℃, preserving heat, filtering, washing, drying, and collecting a dried substance;

(5) and (3) soaking the dried substance in a hydrochloric acid solution, filtering, washing and drying to obtain basic particles, uniformly stirring the basic particles, the auxiliary material, the Arabic gum and the leacheate according to the mass ratio of 10:3:5: 20-25, standing, and freeze-drying to obtain the probiotic additive for the pig feed.

Preferably, the nutrient solution in the step (1) is prepared by taking 230-330 parts of water, 50-60 parts of peptone, 20-30 parts of yeast extract, 10-13 parts of diammonium citrate, 8-12 parts of glucose and 6-9 parts of sodium chloride by weight, uniformly stirring, and sterilizing and disinfecting.

Preferably, the mixed microbial inoculum in the step (1) is prepared by mixing bacillus subtilis and lactobacillus according to any mass ratio.

Preferably, the additive in the step (2) is prepared by mixing disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium chloride according to the mass ratio of 6:5: 1-3.

Preferably, in the step (4), the surfactant is sodium stearate and lecithin which are mixed according to any mass ratio.

Preferably, the auxiliary materials in the step (5) are peptone and glucose which are mixed according to the mass ratio of 10: 1.

Compared with other methods, the method has the beneficial technical effects that:

the invention takes fresh pig intestines as a basic raw material, original ecological microbial communities in the fresh pig intestines are cultured by nutrient solution to obtain dominant strains, the bacteria can effectively adapt to the internal environment of the domestic pig, the regulation of the internal environment of the domestic pig is increased, the colonization of the microbial inoculum in intestinal tracts is improved, meanwhile, mixed microbial inoculum which is beneficial to increasing the internal advantages of the domestic pig is added, the regulating function of an additive on the domestic pig is improved by matching the cultured eluted bacterial solution, then, a porous microsphere structure is prepared by dietary fibers, finally, auxiliary materials are adsorbed by the porous microsphere structure and are adsorbed on the surface of the microsphere by the microorganisms, the auxiliary materials are utilized to provide nutrient substances for the microorganisms, the stable survival of the microorganisms is ensured, and the intestinal tracts can be promoted to promote the intestinal tract movement and the effect by the gum arabic being wrapped and the existence of the dietary fibers, the probiotic additive for the pig feed can effectively solve the problems that the colonization performance of active probiotics in the pig feed in the intestinal tract is weak, the disease resistance of domestic pigs cannot be increased, and the pigs cannot be conditioned.

Detailed Description

The nutrient solution is prepared by uniformly stirring 230-330 parts of water, 50-60 parts of peptone, 20-30 parts of yeast extract, 10-13 parts of diammonium citrate, 8-12 parts of glucose and 6-9 parts of sodium chloride in parts by weight, and sterilizing and disinfecting.

The mixed microbial inoculum is prepared by mixing bacillus subtilis and lactobacillus according to any mass ratio.

The additive is prepared by mixing disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium chloride according to the mass ratio of 6:5: 1-3.

The surfactant is prepared by mixing sodium stearate and lecithin according to any mass ratio.

The auxiliary material is formed by mixing peptone and glucose according to the mass ratio of 10: 1.

A preparation method of a probiotic additive for pig feed comprises the following steps:

(1) putting fresh pork intestines and the nutrient solution into a grinder according to the mass ratio of 1: 10-15, grinding for 30min at the speed of 800r/min, adjusting the pH value to 6-7, collecting the ground matter, standing the ground matter at the temperature of 25-30 ℃ overnight, filtering, collecting filtrate, and uniformly mixing the filtrate and the mixed microbial inoculum according to the mass ratio of 15: 1-3 to obtain base solution;

(2) taking 80-90 parts by weight of peptone, 45-50 parts by weight of agar, 15-25 parts by weight of water, 5-10 parts by weight of glucose and 3-12 parts by weight of additives, uniformly stirring, sterilizing and disinfecting to obtain a solid nutrient, and uniformly mixing the solid nutrient and a base solution according to a mass ratio of 18: 7-9 to obtain a culture;

(3) placing the culture at 28-35 ℃ and the air humidity of 60-65%, culturing for 1-2 days, then leaching the surface of the culture by using distilled water until no hyphae exist on the surface, and collecting leacheate;

(4) uniformly mixing dietary fiber, calcium carbonate and water according to the mass ratio of 3:2: 3-5, adding acetone with the same volume as water, adding a surfactant accounting for 20-30% of the mass of the calcium carbonate, performing ultrasonic oscillation for 30min, adding liquid paraffin accounting for 80-85% of the volume of the water, stirring for 2h at 700r/min, heating to 30 ℃, preserving heat for 15h, filtering, collecting a filter cake, washing with petroleum ether, drying, and collecting a dried substance;

(5) and (3) soaking the dried substance in 1.2mol/L hydrochloric acid solution for 1h, filtering, washing and drying to obtain basic particles, uniformly stirring the basic particles, the auxiliary materials, the Arabic gum and the leacheate according to the mass ratio of 10:3:5: 20-25, standing at room temperature for 10h, and freeze-drying to obtain the probiotic additive for the pig feed.

Example 1

The nutrient solution is prepared by taking 330 parts of water, 60 parts of peptone, 30 parts of yeast extract, 13 parts of diammonium citrate, 12 parts of glucose and 9 parts of sodium chloride in parts by weight, uniformly stirring, and sterilizing and disinfecting.

The mixed microbial inoculum is prepared by mixing bacillus subtilis and lactobacillus according to any mass ratio.

The additive is prepared by mixing disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium chloride according to the mass ratio of 6:5: 3.

The surfactant is prepared by mixing sodium stearate and lecithin according to any mass ratio.

The auxiliary material is formed by mixing peptone and glucose according to the mass ratio of 10: 1.

A preparation method of a probiotic additive for pig feed comprises the following steps:

(1) putting fresh pork intestines and the nutrient solution into a grinder according to the mass ratio of 1: 15, grinding for 30min at the speed of 800r/min, adjusting the pH value to 7, collecting ground materials, standing the ground materials at the temperature of 30 ℃ overnight, filtering, collecting filtrate, and uniformly mixing the filtrate and the mixed microbial inoculum according to the mass ratio of 15: 3 to obtain base solution;

(2) taking 90 parts of peptone, 50 parts of agar, 25 parts of water, 10 parts of glucose and 12 parts of additives in parts by weight, uniformly stirring, sterilizing and disinfecting to obtain solid nutrient, and uniformly mixing the solid nutrient and base liquid in a mass ratio of 18: 9 to obtain a culture;

(3) culturing the culture at 35 deg.C with air humidity of 65% for 2 days, rinsing the surface of the culture with distilled water until no mycelium exists on the surface, and collecting eluate;

(4) uniformly mixing dietary fiber, calcium carbonate and water according to a mass ratio of 3:2: 5, adding acetone with the same volume as water, adding a surfactant accounting for 30% of the mass of the calcium carbonate, performing ultrasonic oscillation for 30min, adding liquid paraffin accounting for 85% of the volume of the water, stirring for 2h at 700r/min, heating to 30 ℃, preserving heat for 15h, filtering, collecting a filter cake, washing with petroleum ether, drying, and collecting a dried substance;

(5) and (3) soaking the dried substance in 1.2mol/L hydrochloric acid solution for 1h, filtering, washing and drying to obtain basic particles, uniformly stirring the basic particles, the auxiliary materials, the Arabic gum and the leacheate according to the mass ratio of 10:3:5: 25, standing at room temperature for 10h, and freeze-drying to obtain the probiotic additive for the pig feed.

Example 2

The nutrient solution is prepared by taking 280 parts of water, 55 parts of peptone, 25 parts of yeast extract, 12 parts of diammonium citrate, 10 parts of glucose and 8 parts of sodium chloride in parts by weight, uniformly stirring, and sterilizing and disinfecting.

The mixed microbial inoculum is prepared by mixing bacillus subtilis and lactobacillus according to any mass ratio.

The additive is prepared by mixing disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium chloride according to the mass ratio of 6:5: 2.

The surfactant is prepared by mixing sodium stearate and lecithin according to any mass ratio.

The auxiliary material is formed by mixing peptone and glucose according to the mass ratio of 10: 1.

A preparation method of a probiotic additive for pig feed comprises the following steps:

(1) putting fresh pork intestines and the nutrient solution into a grinder according to the mass ratio of 1:13, grinding for 30min at the speed of 800r/min, adjusting the pH value to 6.5, collecting ground materials, standing the ground materials at the temperature of 28 ℃ overnight, filtering, collecting filtrate, and uniformly mixing the filtrate and the mixed microbial inoculum according to the mass ratio of 15:2 to obtain base solution;

(2) taking 85 parts of peptone, 48 parts of agar, 20 parts of water, 8 parts of glucose and 8 parts of additives in parts by weight, uniformly stirring, sterilizing and disinfecting to obtain solid nutrient, and uniformly mixing the solid nutrient and base liquid according to the mass ratio of 18:8 to obtain a culture;

(3) culturing the culture at 32 deg.C with air humidity of 63% for 1.5 days, rinsing the surface of the culture with distilled water until no mycelium exists on the surface, and collecting eluate;

(4) uniformly mixing dietary fiber, calcium carbonate and water according to a mass ratio of 3:2:4, adding acetone with the same volume as water, adding a surfactant accounting for 25% of the mass of the calcium carbonate, performing ultrasonic oscillation for 30min, adding liquid paraffin accounting for 83% of the volume of the water, stirring for 2h at 700r/min, heating to 30 ℃, preserving heat for 15h, filtering, collecting a filter cake, washing with petroleum ether, drying, and collecting a dried substance;

(5) and (3) soaking the dried substance in 1.2mol/L hydrochloric acid solution for 1h, filtering, washing and drying to obtain basic particles, uniformly stirring the basic particles, the auxiliary materials, the Arabic gum and the leacheate according to the mass ratio of 10:3:5:23, standing at room temperature for 10h, and freeze-drying to obtain the probiotic additive for the pig feed.

Example 3

The nutrient solution is prepared by uniformly stirring 230 parts of water, 50 parts of peptone, 20 parts of yeast extract, 10 parts of diammonium citrate, 8 parts of glucose and 6 parts of sodium chloride in parts by weight, and sterilizing.

The mixed microbial inoculum is prepared by mixing bacillus subtilis and lactobacillus according to any mass ratio.

The additive is prepared by mixing disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium chloride according to the mass ratio of 6:5: 1.

The surfactant is prepared by mixing sodium stearate and lecithin according to any mass ratio.

The auxiliary material is formed by mixing peptone and glucose according to the mass ratio of 10: 1.

A preparation method of a probiotic additive for pig feed comprises the following steps:

(1) putting fresh pork intestines and the nutrient solution into a grinder according to the mass ratio of 1:10, grinding for 30min at the speed of 800r/min, adjusting the pH value to 6, collecting ground materials, standing the ground materials at the temperature of 25 ℃ overnight, filtering, collecting filtrate, and uniformly mixing the filtrate and the mixed microbial inoculum according to the mass ratio of 15:1 to obtain base solution;

(2) taking 80 parts of peptone, 45 parts of agar, 15 parts of water, 5 parts of glucose and 3 parts of additives by weight, uniformly stirring, sterilizing and disinfecting to obtain solid nutrient, and uniformly mixing the solid nutrient and base liquid according to the mass ratio of 18:7 to obtain a culture;

(3) culturing the culture at 28 deg.C with air humidity of 60% for 1 day, rinsing the culture surface with distilled water until no mycelium exists on the surface, and collecting eluate;

(4) uniformly mixing dietary fiber, calcium carbonate and water according to a mass ratio of 3:2:3, adding acetone with the same volume as water, adding a surfactant accounting for 20% of the mass of the calcium carbonate, performing ultrasonic oscillation for 30min, adding liquid paraffin accounting for 80% of the volume of the water, stirring for 2h at 700r/min, heating to 30 ℃, preserving heat for 15h, filtering, collecting a filter cake, washing with petroleum ether, drying, and collecting a dried substance;

(5) and (3) soaking the dried substance in 1.2mol/L hydrochloric acid solution for 1h, filtering, washing and drying to obtain basic particles, uniformly stirring the basic particles, the auxiliary materials, the Arabic gum and the leacheate according to the mass ratio of 10:3:5:20, standing at room temperature for 10h, and freeze-drying to obtain the probiotic additive for the pig feed.

Comparative example 1

The mixed microbial inoculum is prepared by mixing bacillus subtilis and lactobacillus according to any mass ratio.

The additive is prepared by mixing disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium chloride according to the mass ratio of 6:5: 2.

The surfactant is prepared by mixing sodium stearate and lecithin according to any mass ratio.

The auxiliary material is formed by mixing peptone and glucose according to the mass ratio of 10: 1.

A preparation method of a probiotic additive for pig feed comprises the following steps:

(1) uniformly mixing water and the mixed microbial inoculum according to the mass ratio of 15:2 to obtain a base solution;

(2) taking 85 parts of peptone, 48 parts of agar, 20 parts of water, 8 parts of glucose and 8 parts of additives in parts by weight, uniformly stirring, sterilizing and disinfecting to obtain solid nutrient, and uniformly mixing the solid nutrient and base liquid according to the mass ratio of 18:8 to obtain a culture;

(3) culturing the culture at 32 deg.C with air humidity of 63% for 1.5 days, rinsing the surface of the culture with distilled water until no mycelium exists on the surface, and collecting eluate;

(4) uniformly mixing dietary fiber, calcium carbonate and water according to a mass ratio of 3:2:4, adding acetone with the same volume as water, adding a surfactant accounting for 25% of the mass of the calcium carbonate, performing ultrasonic oscillation for 30min, adding liquid paraffin accounting for 83% of the volume of the water, stirring for 2h at 700r/min, heating to 30 ℃, preserving heat for 15h, filtering, collecting a filter cake, washing with petroleum ether, drying, and collecting a dried substance;

(5) and (3) soaking the dried substance in 1.2mol/L hydrochloric acid solution for 1h, filtering, washing and drying to obtain basic particles, uniformly stirring the basic particles, the auxiliary materials, the Arabic gum and the leacheate according to the mass ratio of 10:3:5:23, standing at room temperature for 10h, and freeze-drying to obtain the probiotic additive for the pig feed.

Comparative example 2

The nutrient solution is prepared by taking 280 parts of water, 55 parts of peptone, 25 parts of yeast extract, 12 parts of diammonium citrate, 10 parts of glucose and 8 parts of sodium chloride in parts by weight, uniformly stirring, and sterilizing and disinfecting.

The mixed microbial inoculum is prepared by mixing bacillus subtilis and lactobacillus according to any mass ratio.

The additive is prepared by mixing disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium chloride according to the mass ratio of 6:5: 2.

The surfactant is prepared by mixing sodium stearate and lecithin according to any mass ratio.

The auxiliary material is formed by mixing peptone and glucose according to the mass ratio of 10: 1.

A preparation method of a probiotic additive for pig feed comprises the following steps:

(1) putting fresh pork intestines and the nutrient solution into a grinder according to the mass ratio of 1:13, grinding for 30min at the speed of 800r/min, adjusting the pH value to 6.5, collecting ground materials, standing the ground materials at the temperature of 28 ℃ overnight, filtering, collecting filtrate, and uniformly mixing the filtrate and the mixed microbial inoculum according to the mass ratio of 15:2 to obtain base solution;

(2) taking 85 parts of peptone, 48 parts of agar, 20 parts of water, 8 parts of glucose and 8 parts of additives in parts by weight, uniformly stirring, sterilizing and disinfecting to obtain solid nutrient, and uniformly mixing the solid nutrient and base liquid according to the mass ratio of 18:8 to obtain a culture;

(3) culturing the culture at 32 deg.C with air humidity of 63% for 1.5 days, rinsing the surface of the culture with distilled water until no mycelium exists on the surface, and collecting eluate;

(4) uniformly stirring the auxiliary material, the Arabic gum and the leacheate according to the mass ratio of 3:5:23, standing at room temperature for 10 hours, and freeze-drying to obtain the probiotic additive for the pig feed.

Adding the probiotic additives for the pig feeds prepared in the examples 1-3 and the comparative examples 1-2 into the basic pig feed according to the addition amount of 2 mass percent, and adding no probiotic additives for the pig feeds into the blank control, normally feeding the domestic pigs, feeding 30 pigs per group for 30 days on average, and detecting results as follows:

	example 1	Example 2	Example 3	Comparative example 1	Comparative example 2	Blank control
							Average daily food intake/kg	1.53	1.62	1.53	0.92	1.11	0.89
Average daily weight gain/kg	0.61	0.72	0.65	0.50	0.55	0.49
							Rate of diarrhea/%)	2.6	2.1	2.3	4.6	4.3	22
Total incidence/%)	3.2	2.6	2.9	4.5	5.2	31.3

In conclusion, the probiotic additive for the pig feed prepared by the invention has better effect.