阅读说明：本技术 一种利用南极磷虾脂质制备的宠物营养膏及其制备方法 (Pet nutrition paste prepared from euphausia superba lipid and preparation method thereof ) 是由 周庆新 谷彩霞 袁溢旋 杨柳 王润雨 陈芳甜 于 2020-12-24 设计创作，主要内容包括：本发明涉及宠物饲料技术领域,具体涉及一种利用南极磷虾脂质制备的宠物营养膏及其制备方法,所述宠物营养膏的制备方法包括南极磷虾脂质制备、南极磷虾脂质递送体系设计、营养膏赋型基质构建和营养膏混合均质。本发明以南极磷虾资源的全方位综合利用为出发点,鉴于南极磷虾中功能脂质的种类-功能-适口性等之间的相互关系,建立南极磷虾中功能脂质的富集方法,同时设计科学的功能脂质营养递送体系,以提高功能脂质的生物学效价,并结合配方基质效应和赋型设计,制备一种营养、功效和适口性良好的富含南极磷虾功能脂质的营养膏产品,推动高端宠物营养品的发展,并以此提高南极磷虾的综合利用途径,拓宽应用领域。(The invention relates to the technical field of pet feed, and particularly relates to a pet nutrition paste prepared from antarctic krill lipid and a preparation method thereof. The invention takes the comprehensive utilization of antarctic krill resources as a starting point, establishes the enrichment method of the functional lipids in the antarctic krill in view of the interrelation between the types, functions, palatability and the like of the functional lipids in the antarctic krill, simultaneously designs a scientific functional lipid nutrition delivery system to improve the biological potency of the functional lipids, and combines the formula matrix effect and the forming design to prepare a nutritional paste product which is good in nutrition, efficacy and palatability and is rich in the antarctic krill functional lipids, promotes the development of high-end pet nutriments, improves the comprehensive utilization approach of the antarctic krill and widens the application field.)

1. A method for preparing a pet nutrition paste by using Antarctic krill shrimp meal lipid is characterized by comprising the following steps:

s1 Antarctic krill lipid preparation: adding an extraction solvent into the euphausia superba powder, collecting an extracting solution after extraction to obtain crude euphausia superba lipid, adding an ethylene diamine tetraacetic acid aqueous solution into the crude euphausia superba lipid, fully stirring and dissolving, concentrating, and separating a concentrated solution oil phase to obtain the euphausia superba lipid;

s2 Antarctic krill lipid delivery system design: adding an emulsifier, an auxiliary emulsifier and a protective agent into the antarctic krill lipid, and fully emulsifying and dispersing the antarctic leaching lipid;

s3 construction of a forming matrix of the nutritional paste: the nutritional paste forming matrix comprises a water system base material and an oil system material, and the water system base material and the oil system material are mixed to form an oil-in-water system, so that the nutritional paste forming matrix is obtained;

s4 mixing and homogenizing the nutrient paste: antarctic krill lipid delivery systems were added to a delivery nutritional paste forming matrix.

2. The method of claim 1, wherein the S1 Euphausia superba lipid preparation is specifically:

(1) coarse extraction: adding an extraction solvent into the Antarctic krill powder, wherein the volume ratio of the extraction solvent to the Antarctic krill powder is 2-10: 1, extracting for 1-3 times for 0.5-4 h, and concentrating the extracting solution under reduced pressure to obtain crude antarctic krill lipid;

the extraction solvent is an ethanol water solution with the concentration of 0.85-1 g/L;

(2) refining: adding an ethylene diamine tetraacetic acid aqueous solution with the concentration of 0.001-0.1 g/L into the crude antarctic krill lipid, wherein the mass ratio of the ethylene diamine tetraacetic acid to the crude antarctic krill lipid is 5-10: and 100, stirring and dissolving, concentrating under the conditions of vacuum degree of-0.06-0.09 MPa and temperature of 40-90 ℃, and centrifuging the concentrated solution to obtain an oil phase, namely the antarctic krill lipid.

3. The method of claim 1, wherein the S2 Antarctic krill lipid delivery system is specifically designed to:

sequentially adding an emulsifier, a co-emulsifier and a protective agent into the Euphausia superba lipid, wherein the mass ratio of the emulsifier to the co-emulsifier to the protective agent to the Euphausia superba lipid is (0.5-10): 0.01-5: 0.001-0.1: 100, and emulsifying and dispersing at 40-70 ℃;

in the S2 Antarctic krill lipid delivery system design, the emulsifier comprises at least one of Tween 80 and polyglycerol fatty acid ester, the co-emulsifier comprises at least one of ethylene glycol, 1, 2-propylene glycol, 1, 3-propylene glycol and glycerol, and the protective agent comprises at least one of rosemary, coenzyme Q10, vitamin E and vitamin E acetate;

in the S2 Antarctic krill lipid delivery system design, the emulsification mode is at least one of shearing homogenizing emulsification, ultrasonic homogenizing emulsification and high-pressure microjet homogenizing emulsification, the rotation speed of the shearing homogenizing emulsification is 1000-30000 rpm, and the time is 1-10 min;

the power of the ultrasonic homogeneous emulsification is 100-900 w, and the time is 0.5-10 min;

the pressure of the high-pressure microjet homogenizing and emulsifying is 40-120 MPa, and the treatment times are 1-3 times.

4. The method of claim 1, wherein the S3 nutritional paste forming matrix is configured to:

(1) preparing a water system base material: weighing 100 parts of water, 3-10 parts of excipient, 10-30 parts of peptone, 5-20 parts of hydrolyzed animal protein, 10-20 parts of milk powder, 10-20 parts of glucose, 5-10 parts of maltose, 10-20 parts of white granulated sugar and 0.5-2 parts of vitamin according to parts by weight, heating and dissolving at 40-90 ℃ after complete hydration, and uniformly stirring to prepare a water system base material;

(2) preparing oil materials: weighing 100 parts of animal fat, 1-10 parts of emulsifier, 0.1-5 parts of co-emulsifier and 0.1-2 parts of fat-soluble vitamin according to parts by weight, and uniformly stirring to prepare an oil material;

(3) mixing the forming base of the nutrient paste: respectively heating a water system base material and an oil system material to 70-100 ℃, and then adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.1-0.5, and homogenizing and emulsifying while adding until the system is uniform to form a stable oil-in-water system without free oil materials, thereby obtaining the forming matrix of the nutritional paste.

5. The method of claim 4, wherein in the S3 nutritional paste excipient matrix construction, the excipient comprises at least one of pectin, sodium alginate, gelatin, agar, and xanthan gum, the animal fat comprises at least one of fish oil, shrimp sauce, pork, chicken, beef tallow, and mutton fat, the emulsifier comprises at least one of sucrose ester, polyglycerol stearate, monoglyceride, diglycerol fatty acid ester, tween, and lecithin, and the co-emulsifier comprises at least one of ethanol, 1, 2-propylene glycol, 1, 3-propylene glycol, and glycerol;

the emulsification method is shearing homogenizing emulsification, the rotating speed of the shearing homogenizing emulsification is 1000-30000 rpm, and the time is 5-30 min.

6. The method of claim 1, wherein the S4 nutritional paste mixing and homogenizing specifically comprises:

cooling the nutritional paste forming matrix to 60-70 ℃, then adding the antarctic krill lipid delivery system into the nutritional paste forming matrix, and performing homogeneous emulsification while adding until the paste is uniform and stable, wherein the mass fraction of the antarctic krill lipid in the finally obtained nutritional paste is not less than 2%.

7. The method of claim 1, wherein the method comprises S5 sterilizing, packaging: sterilizing the nutrient paste, and subpackaging according to production requirements.

8. The method of claim 7, wherein the S5 sterilization and packaging specifically comprises: and (3) preserving heat and sterilizing at 65-70 ℃ for 15min, and then subpackaging according to production requirements.

9. A pet nutrition paste prepared by the method of claim 1.

Technical Field

The invention relates to the technical field of pet feeds, and particularly relates to a pet nutrition paste prepared from antarctic krill lipid and a preparation method thereof.

Background

Pets, also known as "companion animals," play an increasingly important role in our lives as important human emotional setbacks, often viewed as one of the family members. Their nutrition and health have become a prominent challenge. Particularly, with the social progress, the concept of pet feeding is greatly changed, and the diet requirements of people for pets are higher and higher. Therefore, the pet food industry is in need of transformation and upgrading, particularly, the innovation of products is needed, and a pet food industry chain which is more nutritional, healthier and more targeted is developed according to different types and requirements of pets.

The biological reserves of the antarctic krill are huge, and the antarctic krill are listed as important strategic resources in various countries, and belong to one of three extremely important marine resources to be developed. The euphausia superba oil is the most representative of a plurality of products developed by taking the euphausia superba as a raw material. In recent years, Antarctic krill oil has attracted attention because of its unique composition and functional properties. Especially higher polyunsaturated fatty acid (DHA, EPA, etc.), astaxanthin/ester, phospholipid, fat-soluble vitamin, sterol, etc., so that the compound has multiple clinical effects and great development value. However, most of residues left after the Antarctic krill oil is extracted are used as feed raw materials, and the added value of products is low.

Based on the above, the pet nutrition paste product which takes the euphausia superba powder as the raw material, is easy to digest and absorb by pets and is practical and the preparation method thereof have important significance for widening the comprehensive development approach of the euphausia superba resource.

Disclosure of Invention

The invention provides a pet nutrition paste prepared by using Antarctic krill lipid and a preparation method thereof, aiming at the technical problem that the existing pet feed is difficult to meet the feeding requirement.

In a first aspect, the present invention provides a method for preparing a pet nutritional paste using krill meal lipids from Antarctic krill, the method comprising:

s1 Antarctic krill lipid preparation: adding an extraction solvent into the euphausia superba powder, collecting an extracting solution after extraction to obtain crude euphausia superba lipid, adding an ethylene diamine tetraacetic acid aqueous solution into the crude euphausia superba lipid, fully stirring and dissolving, concentrating, and separating a concentrated solution oil phase to obtain the euphausia superba lipid;

s2 Antarctic krill lipid delivery system design: adding an emulsifier, an auxiliary emulsifier and a protective agent into the antarctic krill lipid, and fully emulsifying and dispersing the antarctic leaching lipid;

s3 construction of a forming matrix of the nutritional paste: the nutritional paste forming matrix comprises a water system base material and an oil system material, and the water system base material and the oil system material are mixed to form an oil-in-water system, so that the nutritional paste forming matrix is obtained;

s4 mixing and homogenizing the nutrient paste: antarctic krill lipid delivery systems were added to a delivery nutritional paste forming matrix.

Further, the preparation of the S1 antarctic krill lipid specifically comprises the following steps:

(1) coarse extraction: adding an extraction solvent into the Antarctic krill powder, wherein the volume ratio of the extraction solvent to the Antarctic krill powder is 2-10: 1, extracting for 1-3 times for 0.5-4 h, and concentrating the extracting solution under reduced pressure to obtain crude antarctic krill lipid;

(2) refining: adding an ethylene diamine tetraacetic acid aqueous solution with the concentration of 0.001-0.1 g/L into the crude antarctic krill lipid, wherein the mass ratio of the ethylene diamine tetraacetic acid to the crude antarctic krill lipid is 5-10: and 100, stirring and dissolving, concentrating under the conditions of vacuum degree of-0.06-0.09 MPa and temperature of 40-90 ℃, and centrifuging the concentrated solution to obtain an oil phase, namely the antarctic krill lipid.

Furthermore, in the preparation of the S1 Antarctic krill lipid, the extraction solvent is an ethanol water solution with the concentration of 0.85-1 g/L.

Further, the S2 antarctic krill lipid delivery system is specifically designed as follows:

sequentially adding an emulsifier, a co-emulsifier and a protective agent into the Euphausia superba lipid, wherein the mass ratio of the emulsifier to the co-emulsifier to the protective agent to the Euphausia superba lipid is (0.5-10): 0.01-5: 0.001-0.1: 100, and emulsifying and dispersing at 40-70 ℃.

Further, in the S2 antarctic krill lipid delivery system design, the emulsifier includes at least one of tween 80 and polyglycerol fatty acid ester, the co-emulsifier includes at least one of ethylene glycol, 1, 2-propylene glycol, 1, 3-propylene glycol and glycerol, and the protective agent includes at least one of rosemary, coenzyme Q10, vitamin E and vitamin E acetate.

Further, in the S2 Antarctic krill lipid delivery system design, the emulsification mode is at least one of shearing homogenizing emulsification, ultrasonic homogenizing emulsification and high-pressure microjet homogenizing emulsification, the rotation speed of the shearing homogenizing emulsification is 1000-30000 rpm, and the time is 1-10 min;

the power of the ultrasonic homogeneous emulsification is 100-900 w, and the time is 0.5-10 min;

the pressure of the high-pressure microjet homogenizing and emulsifying is 40-120 MPa, and the treatment times are 1-3 times.

Further, the S3 nutritional paste forming matrix is specifically constructed by the following steps:

(1) preparing a water system base material: weighing 100 parts of water, 3-10 parts of excipient, 10-30 parts of peptone, 5-20 parts of hydrolyzed animal protein, 10-20 parts of milk powder, 10-20 parts of glucose, 5-10 parts of maltose, 10-20 parts of white granulated sugar and 0.5-2 parts of vitamin according to parts by weight, heating and dissolving at 40-90 ℃ after complete hydration, and uniformly stirring to prepare a water system base material;

(2) preparing oil materials: weighing 100 parts of animal fat, 1-10 parts of emulsifier, 0.1-5 parts of co-emulsifier and 0.1-2 parts of fat-soluble vitamin according to parts by weight, and uniformly stirring to prepare an oil material;

(3) mixing the forming base of the nutrient paste: respectively heating a water system base material and an oil system material to 70-100 ℃, and then adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.1-0.5, and homogenizing and emulsifying while adding until the system is uniform to form a stable oil-in-water system without free oil materials, thereby obtaining the forming matrix of the nutritional paste.

Furthermore, in the construction of the S3 nutritional paste excipient matrix, the excipient comprises at least one of pectin, sodium alginate, gelatin, agar and gum arabic, the animal fat comprises at least one of fish oil, shrimp oil, pork, chicken, beef tallow and mutton fat, the emulsifier comprises at least one of sucrose ester, polyglycerol stearate, monoglyceride, diglycerol fatty acid ester, tween and lecithin, and the co-emulsifier comprises at least one of ethanol, 1, 2-propylene glycol, 1, 3-propylene glycol and glycerol.

Furthermore, in the construction of the S3 nutritional paste forming matrix, the emulsification mode is shearing homogenizing emulsification, the rotating speed of the shearing homogenizing emulsification is 1000-30000 rpm, and the time is 5-30 min.

Further, the S4 mixing and homogenizing method specifically comprises the following steps:

cooling the nutritional paste forming matrix to 60-70 ℃, then adding the antarctic krill lipid delivery system into the nutritional paste forming matrix, and performing homogeneous emulsification while adding until the paste is uniform and stable, wherein the mass fraction of the antarctic krill lipid in the finally obtained nutritional paste is not less than 2%. The mass fraction of the antarctic krill lipid in the nutritional paste can be adjusted according to production needs, and can reach up to 20%, and the conventional health care dosage of the pets on polyunsaturated fatty acids (especially phospholipid type DHA/EPA) and astaxanthin can be effectively met.

Further, the method comprises S5 sterilization and packaging: sterilizing the nutrient paste, and subpackaging according to production requirements.

Further, the S5 sterilization and packaging specifically comprises: and (3) preserving heat and sterilizing at 65-70 ℃ for 15min, and then subpackaging according to production requirements.

In a second aspect, the present invention provides a pet nutrition paste prepared according to the above method.

The beneficial effect of the invention is that,

the invention takes the comprehensive utilization of antarctic krill resources as a starting point, establishes the enrichment method of the functional lipids in the antarctic krill in view of the interrelation between the types, functions, palatability and the like of the functional lipids in the antarctic krill, simultaneously designs a scientific functional lipid nutrition delivery system to improve the biological potency of the functional lipids, and combines the formula matrix effect and the forming design to prepare a nutritional paste product which is good in nutrition, efficacy and palatability and is rich in the antarctic krill functional lipids, promotes the development of high-end pet nutriments, improves the comprehensive utilization approach of the antarctic krill and widens the application field.

The pet nutrition paste provided by the invention has the characteristics of natural raw materials, comprehensive nutrition function and rich flavor, is rich in phospholipid and polyunsaturated fatty acid, effectively promotes the development of the brain of a pet, protects the health of optic nerves, enables the hair of the pet to be brighter, improves the immunity, protects the health of skin, enhances the vitality and delays the aging process of the pet; the accelerated test predicts that after the nutritional paste is stored for 12 months at 25 ℃, the retention rate of astaxanthin in the nutritional paste is more than 90%, even more than 95%, the paste is uniform, no oil-water separation phenomenon can be seen by naked eyes, and the stability is good; the bioavailability of DHA and astaxanthin in the nutrient paste is remarkably improved, and the in-vitro three-section mode digestion and absorption test shows that the bioavailability of DHA is more than 90%, and the bioavailability of astaxanthin is more than 70%, which indicates that the DHA and astaxanthin rich in the product can be effectively absorbed and utilized by organisms, and further the biological activity of the DHA and astaxanthin can be fully exerted.

The preparation method of the pet nutrition paste provided by the invention is easy to operate, and the final product has good stability and no water separation phenomenon after long-time storage through designing a formula and optimizing a process; the method is simple and mild, greatly improves the processing convenience of heat-sensitive substances such as astaxanthin and the like, does not generate chemical change, does not change the structure and the physiological activity of the heat-sensitive substances, ensures that the whole process is green and environment-friendly, has low energy consumption, is beneficial to industrial popularization, and has positive significance for promoting the development of high-end pet nutrition functional product industry in China.

Drawings

In order to more clearly illustrate the embodiments or technical solutions in the prior art of the present invention, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.

Fig. 1 is a flow chart of a method for preparing a pet nutrition paste by using antarctic krill lipid in the embodiment of the invention.

Detailed Description

In order to make those skilled in the art better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

A pet nutrition paste prepared from antarctic krill shrimp meal lipid is prepared by the following steps:

s1 Antarctic krill lipid preparation:

(1) coarse extraction: adding an ethanol water solution with the concentration of 0.85g/L into the Antarctic krill powder, wherein the volume ratio of the ethanol water solution to the Antarctic krill powder is 2: 1, extracting for 1 time for 0.5h, and concentrating the extracting solution under reduced pressure to obtain crude antarctic krill lipid;

(2) refining: adding an ethylene diamine tetraacetic acid aqueous solution with the concentration of 0.001g/L into the crude antarctic krill lipid, wherein the mass ratio of the ethylene diamine tetraacetic acid to the crude antarctic krill lipid is 5: 100, stirring and dissolving, concentrating under the conditions of vacuum degree of-0.06 MPa and 40 ℃, and centrifuging the concentrated solution to obtain an oil phase, namely the antarctic krill lipid;

s2 Antarctic krill lipid delivery system design: sequentially adding an emulsifier, a co-emulsifier and a protective agent into the Euphausia superba lipid, wherein the mass ratio of the emulsifier to the co-emulsifier to the protective agent to the Euphausia superba lipid is 0.5: 0.01: 0.001: 100, emulsifying and dispersing at 40 ℃, wherein the emulsifier is Tween 80, the co-emulsifier is ethylene glycol, the protective agent is rosemary, the emulsifying mode is shearing, homogenizing and emulsifying, the rotating speed is 1000rpm, and the time is 1 min;

s3 construction of a forming matrix of the nutritional paste:

(1) preparing a water system base material: weighing 100 parts of water, 3 parts of excipient, 10 parts of peptone, 5 parts of hydrolyzed animal protein, 10 parts of milk powder, 10 parts of glucose, 5 parts of maltose, 10 parts of white granulated sugar and 0.5 part of vitamin according to parts by weight, completely hydrating, heating and dissolving at 40 ℃, and uniformly stirring to prepare a water-based base material, wherein the excipient is pectin;

(2) preparing oil materials: weighing 100 parts of animal fat, 1 part of emulsifier, 0.1 part of co-emulsifier and 0.1 part of fat-soluble vitamin according to the parts by weight, and uniformly stirring to prepare an oil material, wherein the animal fat is fish oil, the emulsifier is sucrose ester, and the co-emulsifier is ethanol;

(3) mixing the forming base of the nutrient paste: respectively heating a water system base material and an oil system material to 70 ℃, and then adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.1, homogenizing and emulsifying while adding until the system is uniform to form a stable oil-in-water system without free oil materials to obtain a nutritional paste forming matrix, wherein the emulsifying mode is shearing homogenizing and emulsifying, the rotating speed of the shearing homogenizing and emulsifying is 1000rpm, and the time is 5 min;

s4 mixing and homogenizing the nutrient paste: cooling the nutritional paste forming matrix to 60 ℃, then adding the antarctic krill lipid delivery system into the nutritional paste forming matrix, and performing homogeneous emulsification while adding until the paste is uniform and stable, wherein the mass fraction of the antarctic krill lipid in the finally obtained nutritional paste is 2%;

s5, sterilization and packaging: sterilizing at 65 deg.C for 15min, and packaging according to production requirement.

Example 1 the eating method of the pet nutrition paste is to eat the paste instantly after uncovering, and about 1-5 g of the paste is taken every day according to the nutrition requirement.

Example 1 sensory indicators of the pet nutritional cream are as follows:

color: the color is brown or reddish brown, and is uniform and consistent;

taste and smell: the flavor is strong, and the product can be melted in the mouth;

the form is as follows: the paste is semisolid, and the phenomenon of phase separation does not occur after the paste is placed for a long time;

impurities: no visible impurities.

Example 2

A pet nutrition paste prepared from antarctic krill shrimp meal lipid is prepared by the following steps:

s1 Antarctic krill lipid preparation:

(1) coarse extraction: adding 1g/L ethanol water solution into the Antarctic krill powder, wherein the volume ratio of the ethanol water solution to the Antarctic krill powder is 10: 1, extracting for 3 times for 4 hours, and concentrating the extracting solution under reduced pressure to obtain crude antarctic krill lipid;

(2) refining: adding an ethylene diamine tetraacetic acid aqueous solution with the concentration of 0.1g/L into the crude antarctic krill lipid, wherein the mass ratio of the ethylene diamine tetraacetic acid to the crude antarctic krill lipid is 10: 100, stirring and dissolving, concentrating under the conditions of vacuum degree of 0.09MPa and 90 ℃, and centrifuging the concentrated solution to obtain an oil phase, namely the antarctic krill lipid;

s2 Antarctic krill lipid delivery system design: sequentially adding an emulsifier, a co-emulsifier and a protective agent into the Euphausia superba lipid, wherein the mass ratio of the emulsifier to the co-emulsifier to the protective agent to the Euphausia superba lipid is 10: 5: 0.1: 100, emulsifying and dispersing at 70 ℃, wherein the emulsifier is polyglycerol fatty acid ester, emulsification is assisted to 1, 2-propylene glycol, the protective agent is vitamin E acetate, the emulsification mode is ultrasonic homogenization emulsification, the power is 600w, and the time is 5 min;

s3 construction of a forming matrix of the nutritional paste:

(1) preparing a water system base material: weighing 100 parts of water, 10 parts of excipient, 30 parts of peptone, 20 parts of hydrolyzed animal protein, 20 parts of milk powder, 20 parts of glucose, 10 parts of maltose, 20 parts of white granulated sugar and 2 parts of vitamin according to parts by weight, heating and dissolving at 90 ℃, and uniformly stirring to prepare a water-system base material, wherein the excipient is sodium alginate;

(2) preparing oil materials: weighing 100 parts of animal fat, 10 parts of emulsifier, 5 parts of co-emulsifier and 2 parts of fat-soluble vitamin according to the parts by weight, and uniformly stirring to prepare an oil material, wherein the animal fat is pork, the emulsifier comprises monoglyceride and diglycerol fatty acid ester, and the co-emulsifier comprises 1, 2-propylene glycol;

(3) mixing the forming base of the nutrient paste: respectively heating a water system base material and an oil system material to 100 ℃, and then adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.5, homogenizing and emulsifying while adding until the system is uniform to form a stable oil-in-water system without free oil materials to obtain a nutritional paste forming matrix, wherein the emulsifying mode is shearing homogenizing and emulsifying, the rotating speed of the shearing homogenizing and emulsifying is 30000rpm, and the time is 30 min;

s4 mixing and homogenizing the nutrient paste: cooling the nutritional paste forming matrix to 70 ℃, then adding the antarctic krill lipid delivery system into the nutritional paste forming matrix, and performing homogeneous emulsification while adding until the paste is uniform and stable, wherein the mass fraction of the antarctic krill lipid in the finally obtained nutritional paste is 20%;

s5, sterilization and packaging: sterilizing at 70 deg.C for 15min, and packaging according to production requirement.

Example 2 the eating method of the pet nutrition paste is to eat the paste instantly after uncovering, and about 1-5 g of the paste is taken every day according to the nutrition requirement.

Example 2 sensory indicators of the pet nutritional cream are as follows:

color: the color is brown or reddish brown, and is uniform and consistent;

taste and smell: the flavor is strong, and the product can be melted in the mouth;

the form is as follows: the paste is semisolid, and the phenomenon of phase separation does not occur after the paste is placed for a long time;

impurities: no visible impurities.

Example 3

A pet nutrition paste prepared from antarctic krill shrimp meal lipid is prepared by the following steps:

s1 Antarctic krill lipid preparation:

(1) coarse extraction: adding 1g/L ethanol water solution into the Antarctic krill powder, wherein the volume ratio of the ethanol water solution to the Antarctic krill powder is 6: 1, extracting for 2 times for 2 hours, and concentrating the extracting solution under reduced pressure to obtain crude antarctic krill lipid;

(2) refining: adding an ethylene diamine tetraacetic acid aqueous solution with the concentration of 0.05g/L into the crude antarctic krill lipid, wherein the mass ratio of the ethylene diamine tetraacetic acid to the crude antarctic krill lipid is 8: 100, stirring and dissolving, concentrating under the conditions of vacuum degree of 0.05MPa and 60 ℃, and centrifuging the concentrated solution to obtain an oil phase, namely the antarctic krill lipid;

s2 Antarctic krill lipid delivery system design: sequentially adding an emulsifier, a co-emulsifier and a protective agent into the Euphausia superba lipid, wherein the mass ratio of the emulsifier to the co-emulsifier to the protective agent to the Euphausia superba lipid is 5: 1: 0.05: 100 and emulsifying and dispersing at 60 ℃, wherein the emulsifier comprises the following components in a volume ratio of 1: 1 of tween 80 and polyglycerol fatty acid ester, wherein the auxiliary emulsifier comprises a mixture of 2: 1, 3-propylene glycol and glycerol, wherein the protective agent comprises the following components in a mass ratio of 1: 1, coenzyme Q10 and vitamin E acetate, wherein the emulsification mode is high-pressure micro-jet homogenization and emulsification, the pressure is 100MPa, and the treatment times are 3 times;

s3 construction of a forming matrix of the nutritional paste:

(1) preparing a water system base material: weighing 100 parts of water, 8 parts of excipient, 15 parts of peptone, 10 parts of hydrolyzed animal protein, 15 parts of milk powder, 15 parts of glucose, 8 parts of maltose, 15 parts of white granulated sugar and 1 part of vitamin according to parts by weight, heating and dissolving at 60 ℃ after complete hydration, and uniformly stirring to prepare a water-system base material, wherein the excipient is agar;

(2) preparing oil materials: weighing 100 parts of animal fat, 5 parts of emulsifier, 2.5 parts of co-emulsifier and 1 part of fat-soluble vitamin according to the parts by weight, and uniformly stirring to prepare an oil material, wherein the animal fat is shrimp oil, and the emulsifier comprises the components in a volume ratio of 1: 3, and the auxiliary emulsifier comprises the components in a volume ratio of 1: 1, 3-propanediol, glycerol;

(3) mixing the forming base of the nutrient paste: respectively heating a water system base material and an oil system material to 80 ℃, and then adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.3, homogenizing and emulsifying while adding until the system is uniform to form a stable oil-in-water system without free oil materials to obtain a nutritional paste forming matrix, wherein the emulsifying mode is shearing homogenizing and emulsifying, the rotating speed of the shearing homogenizing and emulsifying is 10000rpm, and the time is 20 min;

s4 mixing and homogenizing the nutrient paste: cooling the nutritional paste forming matrix to 60 ℃, then adding the antarctic krill lipid delivery system into the nutritional paste forming matrix, and performing homogeneous emulsification while adding until the paste is uniform and stable, wherein the mass fraction of the antarctic krill lipid in the finally obtained nutritional paste is 10%;

s5, sterilization and packaging: sterilizing at 70 deg.C for 15min, and packaging according to production requirement.

Example 3 the eating method of the pet nutrition paste is to eat the paste instantly after uncovering, and about 1-5 g of the paste is taken every day according to the nutrition requirement.

Example 3 sensory indicators of the pet nutritional cream are as follows:

color: the color is brown or reddish brown, and is uniform and consistent;

taste and smell: the flavor is strong, and the product can be melted in the mouth;

the form is as follows: the paste is semisolid, and the phenomenon of phase separation does not occur after the paste is placed for a long time;

impurities: no visible impurities.

Comparative example 1

Comparative example 1 differs from example 3 in that S1 antarctic krill lipid preparation differs, and S1 antarctic krill lipid preparation in comparative example 1 lacks a refining step.

Comparative example 2

Comparative example 2 differs from example 3 in that comparative example 2 lacks the step of S2 antarctic krill lipid delivery system design, and antarctic krill lipids are directly added to the nutritional paste forming matrix and mixed for homogenization.

Test example 1

(1) Total astaxanthin determination

Weighing a certain amount of the samples in the example 3 and the comparative example 1, placing the samples in a glass centrifuge tube with a scale plug, adding 2mL of a mixed solution of dimethyl sulfoxide and acetic acid into the centrifuge tube (a preparation method is that 2.5mL of an acetic acid solution is measured and added into 100mL of the dimethyl sulfoxide solution to obtain a mixed solution of dimethyl sulfoxide and acetic acid), shaking uniformly, shaking and keeping the temperature for 5min under the condition of 70 ℃ water bath, transferring the liquid into a 100mL volumetric flask, fixing the volume to a scale by using the mixed solution of dimethyl sulfoxide and acetic acid, filtering, and measuring the absorbance value at the wavelength of 489nm and marking the absorbance value as A. A mixed solution of dimethyl sulfoxide and acetic acid was used as a blank. The total astaxanthin content in the sample was calculated as follows:

X＝(A_489nm×V×A×10000)/1908×m

in the formula: x is the total content of astaxanthin, mg/kg; a. the_489nmIs the absorbance of the sample at 489 nm; v is the volume of the sample, mL; a is the dilution multiple; 1908 is extinction coefficient; m is the sample mass, g.

(2) Phospholipid assay

2g of samples in example 3 and comparative example 1 are accurately weighed respectively, 25mL of mixed solution of concentrated nitric acid and perchloric acid (concentrated nitric acid: perchloric acid is 4: 1, V/V) is added for soaking, cold digestion is carried out for 2h, ashing is carried out, the mixed solution reacts with ammonium molybdate in the presence of ascorbic acid to generate molybdenum blue, after reaction liquid is properly diluted, the absorbance of the dilution liquid at 650nm is measured, the phospholipid content of the dilution liquid is calculated by a standard curve method, and then the actual phospholipid content is calculated according to the dilution times. The standard curve equation for phospholipids is:

Y＝3.707X-0.026，R＝0.9999

in the formula: x is the absorbance of the sample at 650 nm; y is the concentration of phospholipids in the sample in mg/kg.

The results of the tests by analyzing the main functional components of the samples of example 1 and comparative example 1 are shown in table 1 below.

Table 1 contents of main functional ingredients of example 3 and comparative example 1

Nutritional functional factor	Example 3	Comparative example 1
			Total astaxanthin (mg/1000g x nutritious cream)	31	11
Total phosphate (mg/1000g X nourishing cream)	5204	2053

The test result shows that the content of total astaxanthin and total phospholipid in the nutritional paste prepared by the method for refining and enriching the lipid of the antarctic krill in the embodiment 3 is obviously higher than that in the comparative example 1, which indicates that the nutritional paste prepared by the invention is rich in efficacy factors such as astaxanthin and phospholipid, and can better exert the effect of the nutritional paste.

Test example 2 storage stability test

And (3) taking an equal amount of samples of the example 3 and the comparative example 2, packaging the samples in an aluminum plastic hose, placing the samples in a constant temperature box at 40 ℃ for storage, sampling the samples after 30 days, observing the state of paste, measuring the content of the astaxanthin in the samples, and calculating the retention rate of the astaxanthin in the storage process according to the following formula to evaluate the stability of the samples.

The retention rate (%) of astaxanthin ═ C_n/C₀×100％

Wherein, C_nThe astaxanthin content in the sample after 30 days of storage; c₀The astaxanthin content in the starting sample.

The test results are shown in table 2 below.

Table 2 stability test results of example 3 and comparative example 2

After storage for 30 days, the retention rate of astaxanthin in the sample of example 3 is higher than 90%, which is significantly higher than that of the sample of comparative example 2 (78.6%), and the test results show that: the nutritional paste prepared by the method for designing the delivery system has good stability.

Although the present invention has been described in detail by way of preferred embodiments, the present invention is not limited thereto. Various equivalent modifications or substitutions can be made on the embodiments of the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and these modifications or substitutions are within the scope of the present invention/any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.