阅读说明：本技术 一种甘蔗滤泥发酵菌剂及甘蔗滤泥发酵方法 (Sugarcane filter mud fermentation microbial inoculum and sugarcane filter mud fermentation method ) 是由 李长宁 农倩 林丽 谢金兰 莫璋红 于 2020-12-09 设计创作，主要内容包括：本发明公开了一种甘蔗滤泥发酵菌剂及甘蔗滤泥发酵方法,包括低温菌种、中温菌种和高温菌种的多种复合菌组合成的甘蔗滤泥发酵剂对甘蔗滤泥进行预处理、混合堆料和发酵处理。本发明通过甘蔗滤泥快速生长繁殖,形成微生物发酵优势菌群,使得甘蔗滤泥可快速升温至32-58℃,从而缩短了甘蔗滤泥升温至所需发酵温度的时间,加快了起温速度,从而提高了发酵的效率非常适合在甘蔗滤泥上生长繁殖,并能在气温较低的条件下正常发酵,堆肥温度可达68℃,如此高的温度,提高滤泥中生物酶的转化率；分解作物,上述甘蔗滤泥发酵菌剂,多种菌种以合适的比例配合因而起到温速度快,活性高,高温时间长,因而发酵时间短,发酵完全。(The invention discloses a sugarcane filter mud fermentation microbial inoculum and a sugarcane filter mud fermentation method. According to the invention, the sugarcane filter mud rapidly grows and breeds to form microbial fermentation dominant flora, so that the sugarcane filter mud can be rapidly heated to 32-58 ℃, the time for heating the sugarcane filter mud to the required fermentation temperature is shortened, the heating speed is accelerated, the fermentation efficiency is improved, the sugarcane filter mud is very suitable for growth and breeding on the sugarcane filter mud, the normal fermentation can be carried out under the condition of low air temperature, the composting temperature can reach 68 ℃, and the conversion rate of biological enzymes in the filter mud is improved at such a high temperature; the sugarcane mud-filtering fermentation inoculum is characterized in that a plurality of strains are mixed in a proper proportion to decompose crops, so that the temperature rising speed is high, the activity is high, the high-temperature time is long, the fermentation time is short, and the fermentation is complete.)

1. A sugarcane filter mud fermentation bacterial agent and a sugarcane filter mud fermentation method are characterized in that the sugarcane filter mud fermentation agent comprises a low-temperature strain suitable for growing at 13-35 ℃, a medium-temperature strain suitable for growing at 32-46 ℃ and a high-temperature strain suitable for growing at above 60 ℃, the low-temperature strain is chlamydomonas, the medium-temperature strain is yeast or aspergillus oryzae, the high-temperature strain is thermophilic sporotrichium, the number of chlamydomonas is not less than 1.6 x 106CFU, the number of yeast is not less than 1.6 x 105CFU, the number of aspergillus oryzae is not less than 106CFU, the number of thermophilic sporotrichium is not less than 1.8 x 108CFU, and the total number of bacteria is not less than 1.6 x 109 CFU.

2. The sugarcane filter mud fermentation inoculant and the sugarcane filter mud fermentation method according to claim 1, wherein the method specifically comprises the following steps:

a. pretreatment: adding organic raw materials into the sugarcane filter mud to adjust the water content of the sugarcane filter mud to 50-60% and the carbon-nitrogen ratio of the sugarcane filter mud to 26-29: 1;

b. mixing and stacking: uniformly mixing the sugarcane filter mud leavening agent and the sugarcane filter mud, and then piling the mixture into a stockpile, wherein the mass ratio of the sugarcane filter mud leavening agent to the sugarcane filter mud is 1: 1600-;

c. fermentation: and (3) raising the temperature of the stockpile fermentation to be more than 45-68 ℃ and maintaining for 7-10 days, and then naturally cooling to finish the fermentation.

3. The sugarcane filter mud zymophyte agent and the sugarcane filter mud fermentation method according to claim 1, wherein the mass ratio of chlamydomonas, saccharomycetes, aspergillus oryzae and Thermomyces thermophilus is (15-18): (9-10): (5-9): (5-8).

4. The sugarcane filter mud fermentation inoculum and the sugarcane filter mud fermentation method according to claim 1, wherein in the step c, when the temperature of the compost reaches 60 ℃, the temperature of the compost is controlled not to be higher than 65 ℃ by turning or adjusting the ventilation quantity of the compost, and the turning frequency is that the compost is not turned within 48 hours of the initial mixing and is twice in 1 day thereafter.

Technical Field

The invention relates to the field of fermentation of sugarcane filter mud, in particular to a sugarcane filter mud fermentation microbial inoculum and a sugarcane filter mud fermentation method.

Background

In the process of producing cane sugar, the cane sugar factory can produce some waste materials, such as bagasse, waste molasses, filter mud, leftover materials and the like, besides main products such as white granulated sugar, brown granulated sugar and the like, wherein the filter mud accounts for 2 percent of the total amount of squeezing. The filter mud is difficult to dry naturally due to the characteristics of high water content, difficult ventilation and the like, and is very easy to be polluted by mixed bacteria, and the polluted filter mud enters the soil and further influences the growth of crops; the filter mud is randomly stacked, dumped and buried, land resources are occupied, air is polluted, underground water sources are polluted, the filter mud becomes wastes which are 'placed in a sink and cannot be used', a sugar refinery pays a great deal of filter mud transportation cost and land occupation cost every year, and the filter mud becomes an environmental protection problem which needs to be solved by the sugar refinery and an environmental protection department. At present, the sugarcane filter mud can be quickly decomposed by adding the compound biological agent containing the trichoderma longibrachiatum, so that the sugarcane filter mud can become a biological organic fertilizer absorbed and utilized by crops, and waste is changed into valuable.

The filter mud is generally utilized by a method of adding a zymophyte agent into the filter mud to ferment and decompose the filter mud. However, the common zymophyte agent has slow temperature rise which is generally over three days, the highest temperature is less than 80 ℃, the fermentation period is long, and the decomposition is incomplete.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides a sugarcane filtered mud fermentation microbial inoculum and a sugarcane filtered mud fermentation method.

In order to solve the technical problems, the invention provides the following technical scheme:

the invention relates to a sugarcane mud fermentation inoculant and a sugarcane mud fermentation method, wherein the sugarcane mud fermentation inoculant comprises a low-temperature strain suitable for growing at 13-35 ℃, a medium-temperature strain suitable for growing at 32-46 ℃ and a high-temperature strain suitable for growing at above 60 ℃, the low-temperature strain is chlamydomonas, the medium-temperature strain is yeast or aspergillus oryzae, the high-temperature strain is Thermophilic sporotrichum, in the sugarcane mud fermentation inoculant, the number of chlamydomonas is not less than 1.6 x 106CFU, the number of yeast is not less than 1.6 x 105CFU, the number of aspergillus oryzae is not less than 106CFU, the number of Thermophilic sporotrichum is not less than 1.8 x 108CFU, and the total number of bacteria is not less than 1.6 x 109 CFU.

As a preferred technical scheme of the invention, the method specifically comprises the following steps:

a. pretreatment: adding organic raw materials into the sugarcane filter mud to adjust the water content of the sugarcane filter mud to 50-60% and the carbon-nitrogen ratio of the sugarcane filter mud to 26-29: 1;

b. mixing and stacking: uniformly mixing the sugarcane filter mud leavening agent and the sugarcane filter mud, and then piling the mixture into a stockpile, wherein the mass ratio of the sugarcane filter mud leavening agent to the sugarcane filter mud is 1: 1600-;

c. fermentation: and (3) raising the temperature of the stockpile fermentation to be more than 45-68 ℃ and maintaining for 7-10 days, and then naturally cooling to finish the fermentation.

As a preferred technical scheme of the invention, the mass ratio of the chlamydomonas to the yeast to the aspergillus oryzae to the thermophilic sporotrichum is (15-18): (9-10): (5-9): (5-8).

In a preferred embodiment of the present invention, in the step c, when the temperature of the pile reaches 60 ℃, the temperature of the pile is controlled to be not higher than 65 ℃ by turning or adjusting the ventilation amount of the pile, and the pile turning frequency is that the pile is not turned within 48 hours of the initial mixing, and is twice in 1 day thereafter.

Compared with the prior art, the invention has the following beneficial effects:

1: according to the invention, the sugarcane filter mud rapidly grows and breeds to form a microbial fermentation dominant flora, so that the sugarcane filter mud can be rapidly heated to 32-58 ℃, the time for heating the sugarcane filter mud to the required fermentation temperature is shortened, the heating speed is accelerated, the fermentation efficiency is improved, the sugarcane filter mud is very suitable for growth and breeding on the sugarcane filter mud, the sugarcane filter mud can be normally fermented under the condition of low temperature, the composting temperature can reach 68 ℃, and at such a high temperature, pathogenic bacteria, ova, weed seeds and the like carried in the filter mud can be effectively killed, toxic and harmful substances are degraded, and the conversion rate of biological enzymes in the filter mud is improved; the sugarcane mud-filtering fermentation inoculum is characterized in that a plurality of strains are mixed in a proper proportion to decompose crops, so that the temperature rising speed is high, the activity is high, the high-temperature time is long, the fermentation time is short, and the fermentation is complete.

Detailed Description

The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention.

Example 1

The invention provides a sugarcane mud fermentation inoculant and a sugarcane mud fermentation method, wherein the sugarcane mud fermentation inoculant comprises a low-temperature strain suitable for growing at 13-35 ℃, a medium-temperature strain suitable for growing at 32-46 ℃ and a high-temperature strain suitable for growing at above 60 ℃, the low-temperature strain is chlamydomonas, the medium-temperature strain is yeast or aspergillus oryzae, the high-temperature strain is Thermophilus sp, in the sugarcane mud fermentation inoculant, the number of chlamydomonas is not less than 1.6 multiplied by 106CFU, the number of yeast is not less than 1.6 multiplied by 105CFU, the number of aspergillus oryzae is not less than 106CFU, and the number of Thermophilus sp is not less than 1.8

108CFU, and the total bacteria number is not less than 1.6 × 109 CFU.

The method specifically comprises the following steps:

a. pretreatment: adding organic raw materials into the sugarcane filter mud to adjust the water content of the sugarcane filter mud to 50-60% and the carbon-nitrogen ratio of the sugarcane filter mud to 26-29: 1;

b. mixing and stacking: uniformly mixing the sugarcane filter mud leavening agent and the sugarcane filter mud, and then piling the mixture into a stockpile, wherein the mass ratio of the sugarcane filter mud leavening agent to the sugarcane filter mud is 1: 1600-;

c. fermentation: the temperature of the stockpile fermentation is raised to 13-35 ℃ and maintained for 7-10 days, and then the temperature is naturally reduced, thus completing the fermentation;

specifically, the mass ratio of the chlamydomonas, the saccharomycetes, the aspergillus oryzae and the thermophilic sporotrichum is (15-18): (9-10): (5-9): (5-8), when the temperature of the stack in the step c reaches 30 ℃, controlling the temperature of the stack to be not higher than 35 ℃ by turning or adjusting the ventilation quantity of the stack, wherein the turning frequency is that the stack is not turned within 48 hours after the initial mixing, and the turning frequency is twice in 1 day.

Example 2

The method specifically comprises the following steps:

a. pretreatment: adding organic raw materials into the sugarcane filter mud to adjust the water content of the sugarcane filter mud to 60% and the carbon-nitrogen ratio of the sugarcane filter mud to 25-30: 1;

b. mixing and stacking: uniformly mixing the sugarcane filter mud leavening agent and the sugarcane filter mud, and then piling the mixture into a stockpile, wherein the mass ratio of the sugarcane filter mud leavening agent to the sugarcane filter mud is 1: 1800-2000;

c. fermentation: and (3) raising the temperature of the stockpile fermentation to 32-46 ℃ and maintaining for 7-10 days, and then naturally cooling to finish the fermentation.

Specifically, the mass ratio of the chlamydomonas, the saccharomycetes, the aspergillus oryzae and the thermophilic sporotrichum is (16-18): (8-10): (5-8): (5-8), when the temperature of the stack in the step c reaches 42 ℃, controlling the temperature of the stack to be not higher than 46 ℃ by turning or adjusting the ventilation quantity of the stack, wherein the turning frequency is that the stack is not turned within 48 hours after the initial mixing, and the turning frequency is twice in 1 day;

example 3

The method specifically comprises the following steps:

a. pretreatment: adding organic raw materials into the sugarcane filter mud to adjust the water content of the sugarcane filter mud to 50-60% and the carbon-nitrogen ratio of the sugarcane filter mud to 26-29: 1;

b. mixing and stacking: uniformly mixing the sugarcane filter mud leavening agent and the sugarcane filter mud, and then piling the mixture into a stockpile, wherein the mass ratio of the sugarcane filter mud leavening agent to the sugarcane filter mud is 1: 1600-;

c. fermentation: the temperature of the stockpile fermentation is raised to above 60 ℃ and maintained for 7-10 days, and then natural cooling is started, thus completing the fermentation;

specifically, the mass ratio of the chlamydomonas, the saccharomycetes, the aspergillus oryzae and the thermophilic sporotrichum is (16-19): (8-10): (5-6): (5-8), when the temperature of the stack in the step c reaches 58 ℃, controlling the temperature of the stack to be not higher than 68 ℃ by turning or adjusting the ventilation quantity of the stack, wherein the turning frequency is that the stack is not turned within 48 hours after the initial mixing and is twice in 1 day.

According to the invention, the sugarcane filter mud rapidly grows and breeds to form a microbial fermentation dominant flora, so that the sugarcane filter mud can be rapidly heated to 32-58 ℃, the time for heating the sugarcane filter mud to the required fermentation temperature is shortened, the heating speed is accelerated, the fermentation efficiency is improved, the sugarcane filter mud is very suitable for growth and breeding on the sugarcane filter mud, the sugarcane filter mud can be normally fermented under the condition of low temperature, the composting temperature can reach 68 ℃, and at such a high temperature, pathogenic bacteria, ova, weed seeds and the like carried in the filter mud can be effectively killed, toxic and harmful substances are degraded, and the conversion rate of biological enzymes in the filter mud is improved; the sugarcane mud-filtering fermentation inoculum is characterized in that a plurality of strains are mixed in a proper proportion to decompose crops, so that the temperature rising speed is high, the activity is high, the high-temperature time is long, the fermentation time is short, and the fermentation is complete.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.