阅读说明：本技术 一种螟黄足盘绒茧蜂的室内繁殖方法 (Indoor propagation method of Aphidius gifuensis ) 是由 陆明星 何馥晶 杜予州 于 2020-12-17 设计创作，主要内容包括：本发明涉及一种螟黄足盘绒茧蜂的室内繁殖方法,先将单头4-5龄大螟幼虫放置于指形玻璃管中,接入雌雄比1:1-2:1的螟黄足盘绒茧蜂,再用100-200目细目网纱封住玻璃管口,再在100-200目细目网纱上放上蘸取营养液的脱脂棉球后,用黑布包裹指形玻璃管让螟黄足盘绒茧蜂寄生大螟幼虫；然后将寄生6-8小时后的大螟幼虫从指形玻璃管中取出,再接入未寄生的大螟幼虫到有螟黄足盘绒茧蜂的指形玻璃管中；在螟黄足盘绒茧蜂寄生过程中,如果单个指形玻璃管中部分螟黄足盘绒茧蜂死亡,合并不同指形玻璃管中的螟黄足盘绒茧蜂；最后将被寄生的大螟幼虫取出置于饲养瓶中饲养直至螟黄足盘绒茧蜂从被寄生的大螟幼虫体内啮出；通过本发明,可有效寄生繁殖大螟,有助于螟黄足盘绒茧蜂种群数量的扩繁。(The invention relates to an indoor propagation method of snout moth larva, which comprises the steps of placing single-head larva of the snout moth larva of the large-foot moth larva with age 4-5 in a finger-shaped glass tube, inoculating snout moth larva with male-female ratio of 1:1-2:1, sealing the opening of the glass tube by using 100-plus-200-mesh gauze, placing a degreasing cotton ball dipped with nutrient solution on the 100-plus-200-mesh gauze, and wrapping the finger-shaped glass tube by using black cloth to enable the larva of the large-foot snout moth larva to be parasitized by the snout moth larva of the large-foot moth larva; then, the larvae of the sesamia inferens which are parasitic for 6 to 8 hours are taken out from the finger-shaped glass tube, and the larvae of the sesamia inferens which are not parasitic are inoculated into the finger-shaped glass tube of the sesamia inferens with the yellow foot disc cocoon bee; in the parasitic process of the borer yellow-foot disc cocoon bees, if a part of the borer yellow-foot disc cocoon bees die in a single finger-shaped glass tube, the borer yellow-foot disc cocoon bees in different finger-shaped glass tubes are combined; finally, the parasitized larva of the sesamia inferens is taken out and placed in a feeding bottle to be fed until the sesamia inferens hubner is meshed out from the parasitized larva of the sesamia inferens hubner; the invention can effectively parasitize and propagate the pink borer and is beneficial to the propagation of the population quantity of the borer yellow foot disc cocoon bee.)

1. An indoor propagation method of Aphidius gifuensis Gekko Swinhi is characterized by comprising the following steps:

step 1), placing single-head 4-5-year old sesamiella inferens larvae into a finger-shaped glass tube, inoculating Aphidius gifuensis Georgi with a male-female ratio of 1:1-2:1 by using an insect suction tube, sealing the opening of the glass tube by using 100-mesh gauze with 200 meshes, placing absorbent cotton balls dipped with nutrient solution on the 100-mesh gauze with 200 meshes, and wrapping the finger-shaped glass tube by using black cloth to enable the Aphidius gifuensis Georgi to parasitize the sesamiella inferens larvae for 6-8 hours;

step 2), taking out the larvae of the sesamia inferens which are parasitic for 6 to 8 hours from the finger-shaped glass tube, then inoculating the larvae of the sesamia inferens which are not parasitic into the finger-shaped glass tube with the sesamia inferens Hemsl cocoon bee, and repeating the step until the sesamia inferens Hemsl cocoon bee dies completely;

step 3), in the parasitic process of the borer yellow-foot disc cocoon bee, if a part of the borer yellow-foot disc cocoon bee dies in a single finger-shaped glass tube, the borer yellow-foot disc cocoon bee in different finger-shaped glass tubes is combined to ensure that the borer yellow-foot disc cocoon bee parasitic larva with a proper male-female ratio exists in each finger-shaped glass tube, so that the borer yellow-foot disc cocoon bee is efficiently and quickly propagated;

step 4), taking out the parasitized larva of the sesamia inferens, placing the parasitized larva of the sesamia inferens in a feeding bottle for feeding until the sesamia inferens and the pelamia filiformis are meshed out from the parasitized larva of the sesamia inferens; feeding 15-20 head parasitic larva of Sesamia inferens in each feeding bottle; placing 1-2 pieces of Zizania latifolia at the bottom of the feeding bottle, replacing Zizania latifolia once for 2-3 days, and sealing the feeding bottle mouth with black cloth.

2. The indoor propagation method of the Aphidius gifuensis Koch as claimed in claim 1, wherein the finger-shaped glass tube has the specification: the diameter is 1.5-2cm, and the height is 9.5-10 cm.

3. The indoor propagation method of the Aphidius gifuensis Koch as claimed in claim 1, wherein the specifications of the feeding bottles in the step 4) are as follows: the diameter is 7.5-8cm, and the height is 5.5-6 cm.

4. The indoor propagation method of the borer yellow-foot disc cocoon bee according to the claim 1, characterized in that when the borer yellow-foot disc cocoon bee parasitizes the larva of the borer yellow-foot disc cocoon bee and the parasitized larva of the borer are taken out and put into a breeding bottle for breeding, the finger-shaped glass tube and the breeding bottle are both put into an artificial climate incubator, the artificial climate incubator has the air temperature set at 27 plus or minus 1 ℃, the humidity kept at 75 plus or minus 5%, the illumination L is 16:8, and the light intensity is 1200 lx;

when in parasitism, the finger-shaped glass tube is completely wrapped by black cloth and then put into an incubator;

when breeding the larvae of the sesamia inferens after being parasitized for 6-8h, only the bottle mouth of the transparent glass breeding bottle needs to be sealed by the black cloth; the feeding bottle needs to be filled with absorbent paper to prevent overhigh humidity.

5. The indoor propagation method of the borer yellow-foot disc cotesia fudgensis mason according to claim 1, wherein the male-female parasitic ratio of the borer yellow-foot disc cotesia fudgensis mason is 1:1-2:1, and the death of part of the borer yellow-foot disc cotesia fudgensis mason merges the borer yellow-foot disc cotesia fudgensis mason living in different test tubes so as to ensure that each test tube contains the borer yellow-foot disc cotesia fudgensis mason with the appropriate male-female ratio.

6. The indoor propagation method of the Aphidius gifuensis ashmead as claimed in claim 1, wherein the nutrient solution is 8-12% of sophora japonica honey water, the sophora japonica honey water is soaked in the absorbent cotton ball, and the absorbent cotton ball is placed on 100-mesh and 200-mesh gauze at the test tube opening.

Technical Field

The invention relates to an indoor propagation method of Aphidius gifuensis Gekko Swinhi, and belongs to the field of biological control research of plant protection pests.

Background

Greater borerSesamiainferens(Walker) belonging to Lepidoptera (Lepidotera), Noctuoidae, Heliothis (Heliothis virescens) ((Walker))Sesamia) Is an important boring pest on rice. The rice borer is a secondary pest on rice originallyThe interest on the protein is far lower than that of chilo suppressalis and tryporyza incertulas. However, in recent years, with the global warming and the change of the rice cultivation and planting system, especially the wide popularization of hybrid rice, the population quantity of the sesamia inferens in the paddy field gradually increases, the damage of the sesamia inferens in local areas along the river, the coast and the northwest of Jiangsu is gradually increased year by year, the damage of the sesamia inferens even exceeds that of the chilo suppressalis in the southwest area, the sesamia suppressalis is increased to be a main pest from a secondary pest, the damage is not limited to the edge of the paddy field, and the sesamia suppressalis is found in the center. At present, the main means for controlling the rice stem borer in China is chemical prevention and control, but the 3R problem caused by excessive use of chemical agents and the environmental and agricultural product safety problems are more and more serious, so that the effective and safe green prevention and control technology is inevitably explored.

Biological control has been developed in recent years as an effective and safe green control technology. Natural control of natural enemies to pests and large-scale release of the natural enemies are utilized, particularly different natural enemies of the pests at different growth and development stages are utilized to cooperatively perform green comprehensive control on the pests, and the method is an important means for biological control. China has achieved good results in the aspect of controlling pests by using natural enemies, for example, natural enemies such as trichogramma, encarsia formosa, predatory mites, ladybug, parasitic flies and the like are used to successfully control pests such as corn borer, pine moth, cotton bollworm, whitefly, spider mites, aphids and the like. In addition, while the natural enemy resources of the country are utilized, the natural enemy species are introduced from abroad, and the method plays an important role in controlling related pests.

Borer yellow foot disc floss cocoon bee (CotesiaflavipesCameron) belonging to the order Hymenoptera (Hymenoptera), the family Braconidae (Braconidae), the genus Braconidae: (Combrella)Cotesia) It is a natural enemy of endoparasitic dominant species in the larva stage of the sesamia inferens, and is distributed in a plurality of countries in the world. According to rice field survey in Yangzhou areas, only one kind of the borer yellow-foot disc cocoon bee is found in the endoparasitic bee in the larva period of the sesamia inferens, the parasitic rate of the endoparasitic bee to the sesamia inferens larva in the field reaches 33.33%, and the population growth of the sesamia inferens larva in the field can be effectively controlled. However, the breeding and propagation technology of the parasitic wasps is not reported so far. Therefore, the invention provides an indoor propagation method of the Aphidius gifuensis Gekko Swinhi.

Disclosure of Invention

The invention aims to solve the problems and provides an indoor propagation method of the borer yellow foot disc cotesia fudgensis, and the indoor propagation method of the borer yellow foot disc cotesia fudgensis can be used for rapidly propagating the borer yellow foot disc cotesia fudgensis population indoors.

The technical scheme of the invention is as follows: an indoor propagation method of Aphidius gifuensis Gekko Swinhi is characterized by comprising the following steps:

step 1), placing single-head 4-5-year old sesamiella inferens larvae into a finger-shaped glass tube, inoculating Aphidius gifuensis Georgi with a male-female ratio of 1:1-2:1 by using an insect suction tube, sealing the opening of the glass tube by using 100-mesh gauze with 200 meshes, placing absorbent cotton balls dipped with nutrient solution on the 100-mesh gauze with 200 meshes, and wrapping the finger-shaped glass tube by using black cloth to enable the Aphidius gifuensis Georgi to parasitize the sesamiella inferens larvae for 6-8 hours;

step 2), taking out the larvae of the sesamia inferens which are parasitic for 6 to 8 hours from the finger-shaped glass tube, then inoculating the larvae of the sesamia inferens which are not parasitic into the finger-shaped glass tube with the sesamia inferens Hemsl cocoon bee, and repeating the step until the sesamia inferens Hemsl cocoon bee dies completely;

step 3), in the parasitic process of the borer yellow-foot disc cocoon bee, if a part of the borer yellow-foot disc cocoon bee dies in a single finger-shaped glass tube, the borer yellow-foot disc cocoon bee in different finger-shaped glass tubes is combined to ensure that the borer yellow-foot disc cocoon bee parasitic larva with a proper male-female ratio exists in each finger-shaped glass tube, so that the borer yellow-foot disc cocoon bee is efficiently and quickly propagated;

step 4), taking out the parasitized larva of the sesamia inferens, placing the parasitized larva of the sesamia inferens in a feeding bottle for feeding until the sesamia inferens and the pelamia filiformis are meshed out from the parasitized larva of the sesamia inferens; feeding 15-20 head parasitic larva of Sesamia inferens in each feeding bottle; placing 1-2 pieces of Zizania latifolia at the bottom of the feeding bottle, replacing Zizania latifolia once for 2-3 days, and sealing the feeding bottle mouth with black cloth.

The specification of the finger-shaped glass tube is as follows: the diameter is 1.5-2cm, and the height is 9.5-10 cm.

The specification of the feeding bottle in the step 4) is as follows: the diameter is 7.5-8cm, and the height is 5.5-6 cm.

When the striped callosobruchus flavopodus parasitizes the larva of the pink borer and the parasitized larva of the pink borer are taken out and placed in a breeding bottle for breeding, the finger-shaped glass tube and the breeding bottle are placed in an artificial climate incubator, the temperature is set to be 27 +/-1 ℃, the humidity is kept at 75 +/-5%, the illumination L is 16:8, and the light intensity is 1200 lx;

when in parasitism, the finger-shaped glass tube is completely wrapped by black cloth and then put into an incubator;

when breeding the larvae of the sesamia inferens after being parasitized for 6-8h, only the bottle mouth of the transparent glass breeding bottle needs to be sealed by the black cloth; the feeding bottle needs to be filled with absorbent paper to prevent overhigh humidity.

The male-female parasitic ratio of the breeding borer yellow-foot disc cotesia fuensis is 1:1-2:1, and when part of the borer yellow-foot disc cotesia fuensis dies, the borer yellow-foot disc cotesia fuensis living in different test tubes are combined to ensure that each test tube contains the borer yellow-foot disc cotesia fuensis with the appropriate male-female ratio.

The nutrient solution is 8-12% of sophora flower honey water, the sophora flower honey water is soaked into the absorbent cotton ball, and the absorbent cotton ball is placed on 100-mesh 200-mesh fine-mesh gauze at the opening of the test tube.

The method is advanced and scientific, and comprises the steps of (1) placing single-head 4-5-year old larva of the sesamia inferens in a finger-shaped glass tube, inoculating the sesamia inferens in a male-female ratio of 1:1-2:1 by using an insect suction tube, sealing the opening of the glass tube by using fine-mesh 100-plus-powder 200-mesh gauze, and wrapping the glass tube by using black cloth after nutrient solution is put on the glass tube to parasitize the larva of the sesamia inferens for 6-8 hours; (2) taking out the larva of the sesamia inferens which is parasitic for 6 to 8 hours from the finger-shaped glass tube, inoculating the larva of the sesamia inferens which is not parasitic into the finger-shaped glass tube with the parasitic wasps, and repeating the steps until the sesamia inferens byssus is completely dead; (3) if some parasitic wasps in a single tube (finger-shaped glass tube) die, the borer yellow foot disc cocoon wasps in different finger-shaped glass tubes are combined to ensure that each tube (finger-shaped glass tube) has a proper larva of the borer with a male-female ratio. (4) Taking out the parasitized larva of the sesamia inferens, placing the parasitized larva of the sesamia inferens in a feeding bottle, and feeding 15-20 heads of the parasitized larva of the sesamia inferens in each feeding bottle. Placing 1-2 zizania latifolia blocks at the bottom of the feeding bottle, and sealing the feeding bottle mouth with black cloth; (5) and (4) replacing the cane shoots regularly until the yellow-foot disc cocoon bee of the borer is bitten out of the parasitized larva of the sesamia inferens.

By adopting the method, the sesamia inferens can be effectively parasitized and propagated, the parasitization rate and the emergence rate respectively reach 50 percent and 94 percent, and the method is favorable for the propagation of the population quantity of the sesamia inferens.

Drawings

FIG. 1 is a schematic illustration of the parasitics in a glass finger according to the present invention;

FIG. 2 is a schematic view of the feeding in the feeding bottle of the present invention;

FIG. 3 is a graph showing the effect of different nutrients on the longevity of Aphidius gifuensis Gekko suppressalis;

FIG. 4 is a graph showing the effect of sunflower nectar of different concentrations on the life of a borer yellow foot disc cocoon bee.

In the figure: 1, 2, degreasing cotton balls dipped with nutrient solution, 3, and 4, water bamboo.

Detailed description of the invention

The following embodiments are further illustrative of the present invention with reference to the accompanying drawings.

The specific implementation steps of the indoor propagation technology of the borer yellow foot disc cotesia esculenta comprise:

1. referring to fig. 1, a single-head 4-5-year old borer larva is placed in a finger-shaped glass tube, a moth-killing bracon hebetor with a male-female ratio of 1:1-2:1 is inoculated into the finger-shaped glass tube by using an insect sucking tube, the opening of the glass tube is sealed by using 100-mesh gauze, and after a degreasing cotton ball dipped with nutrient solution is placed, the finger-shaped glass tube is wrapped by black cloth to enable the parasitic wasp to parasitize the larva of the borer for 6-8 hours.

2. And (3) taking out the larvae of the sesamia inferens which are parasitic for 6-8 hours from the finger-shaped glass tube, inoculating the larvae of the sesamia inferens which are not parasitic into the finger-shaped glass tube with the sesamia inferens Hemsl cocoon bee, and repeating the steps until the sesamia inferens Hemsl cocoon bee dies completely.

3. In the parasitic process of the borer yellow-foot disc cocoon bee, if a part of the borer yellow-foot disc cocoon bee dies in a single finger-shaped glass tube, the borer yellow-foot disc cocoon bee in different finger-shaped glass tubes is combined to ensure that each finger-shaped glass tube has a proper male-female ratio parasitic diapause larva, so that the borer yellow-foot disc cocoon bee can be efficiently and quickly propagated.

4. Taking out the parasitized larva of the sesamia inferens, and placing the larva into a breeding bottle for breeding. Each breeding bottle is used for breeding 15-20 head parasitic larva of Sesamia inferens. Placing 1-2 pieces of Zizania latifolia at the bottom of the feeding bottle, replacing Zizania latifolia once for 2-3 days, and sealing the feeding bottle mouth with black cloth (see figure 2). Until the yellow foot disc cocoon bee is bitten out from the parasitized larva of the sesamia inferens.

As shown in fig. 1 and 2, 1-borer yellow foot disc cocoon bee, 2-dipped absorbent cotton ball with nutrient solution, 3-borer and 4-cane shoot.

The selection of the nutrient solution in the present invention is based on the previous experimental data. In order to explore the nutrient source most suitable for breeding the borer yellow-foot disc cotesia fudgensis, the invention explores the influence of different nutrient sources on the service life of the borer yellow-foot disc cotesia fudgensis.

A total of 5 treatments (including control) were set up in this experiment: treatment 1: no nutrient was fed for control treatment; and (3) treatment 2: feeding distilled water; and (3) treatment: feeding 10% of flos Sophorae honey water; and (4) treatment: feeding 10% pollen oral liquid; and (4) treatment 5: feeding 10% linden honey water, providing corresponding nutrient substances every 6-8h, observing and recording the death number of the borer yellow foot disc velvet cocoon bee, and recording the service life of the borer yellow foot disc velvet cocoon bee under different nutrient sources. Each was repeated 3 times.

The research of the test results shows that: the life of the borer yellow foot disc cotesia fudgensis treated by the 3, 4 and 5 is higher whether the female bees or the male bees are higher, and the control group has significant difference (P<0.01); the life of the male and female borer cotesia lutescens in treatment 3 was the longest in the five treatments, about 36 hours. The service life of the borer yellow-foot disc cocoon bee of the treatment 1 is the shortest in five treatments, namely about 6 hours, regardless of a female bee or a male peak; there was no significant difference in the life of the males and females of the borer cotesia lutescens of treatments 2 and 5 (fig. 3). However, it is consistent that the life of the female bee of the borer yellow foot disc cocoon bee is generally higher than the life of the male bee produced by the amphoteric reproduction and parthenogenesis of the borer yellow foot disc cocoon bee in the five treatments, and the life of the male bee produced by the amphoteric reproduction and the male bee produced by parthenogenesis of the bee is not significantly different in the five treatments.

And setting five concentrations of sunflower honey water of 8%, 9%, 10%,11% and 12%, and recording the service life of the five concentrations of sunflower honey water for feeding the Sesamia suppressalis yellow foot disc cocoon bee. Each was repeated 3 times.

The research of the test results shows that: the life of the borer yellow-foot disc cotesia esculenta raised under the five concentrations is no matter the sexual reproductionNo significant difference between production and parthenogenesis (P>0.05) and the lifetime durations are all very close (fig. 4). Also, in these treatments, the life of the female of the borer yellow-foot disc cocoon bee is longer than that of the male of the amphoteric reproduction of the borer yellow-foot disc cocoon bee and the male produced by parthenogenesis.

The reason for finally selecting 8-12% of sophora flower honey water as the nutrient source is as follows: (1) the life of the female and male bombesi crocea walsingham is the longest in five treatments and is obviously different from that of a control group. (2) The life of the borer yellow foot disc cocoon bee bred by the sophora japonica honey water in the concentration range of 8-12% is longer, and no obvious difference exists.