Full-automatic immunoblotting instrument and detection method
阅读说明：本技术 一种全自动免疫印迹仪及检测方法 (Full-automatic immunoblotting instrument and detection method ) 是由 李东琦 于 2020-07-04 设计创作，主要内容包括：本发明涉及一种全自动免疫印迹仪及检测方法,其中,全自动免疫印迹仪包括机架,机架上设置有试剂盒、试剂加注装置,装有膜条的反应器皿、样品盒、用于将样品盒内样本液体加入反应器皿内的样本加注机构,机架上转动设置有同轴线的大盘和小盘,试剂盒有多个且沿小盘的周向设置于小盘上,试剂加注装置位于小盘的外边缘处,反应器皿和样品盒均设置有多个,且均沿大盘的周向设置,样品盒安装于大盘的边缘,反应器皿安装于大盘的中心与样品盒之间,样本加注机构设置于短行程轨道上,且沿着大盘的径向移动,合理、高效利用大盘和小盘的空间构造,整个仪器设备趋向于小型化、高效化和自动化,夹持一次性吸嘴,避免交叉感染。(The invention relates to a full-automatic immunoblotting instrument and a detection method, wherein the full-automatic immunoblotting instrument comprises a rack, a kit, a reagent filling device, a reaction vessel provided with a membrane strip, a sample box and a sample filling mechanism for filling sample liquid in the sample box into the reaction vessel are arranged on the rack, a large disc and a small disc which are coaxial are rotatably arranged on the rack, the kit is provided with a plurality of reagent containers and are arranged on the small disc along the circumferential direction of the small disc, the reagent filling device is positioned at the outer edge of the small disc, the reaction vessel and the sample box are both provided with a plurality of reagent containers and are both arranged along the circumferential direction of the large disc, the sample box is arranged at the edge of the large disc, the reaction vessel is arranged between the center of the large disc and the sample box, the sample filling mechanism is arranged on a short-stroke track and moves along the radial direction of the large disc, the space structure of the large disc and the small disc is reasonably and efficiently, High efficiency and automation, and the disposable suction nozzle is clamped to avoid cross infection.)
1. The utility model provides a full-automatic immunoblotting appearance, includes frame (1), be provided with kit, reagent filling device (13) in frame (1), be equipped with reaction household utensils (6), sample box (8.2) of membrane strip, be used for adding sample liquid in sample box (8.2) sample filling mechanism (16) in reaction household utensils (6), its characterized in that: the kit is characterized in that a large disc (2.1) and a small disc (9) which are coaxial are arranged on the rack (1) in a rotating mode, the small disc (9) is higher than the large disc (2.1) in arrangement, the kit is provided with a plurality of reagent filling devices and arranged on the small disc (9) along the circumferential direction of the small disc (9), the reagent filling devices (13) are located at the outer edge of the small disc (9), the reaction vessels (6) and the sample boxes (8.2) are arranged in a plurality of positions and arranged along the circumferential direction of the large disc (2.1), the sample boxes (8.2) are arranged at the edge of the large disc (2.1), the reaction vessels (6) are arranged between the center of the large disc (2.1) and the sample boxes (8.2), one side, located on the small disc (9), of the rack (1) is provided with a short stroke track (15.8), and the sample filling module is arranged on the short stroke track (15.8) and moves along the radial direction of the large disc (2.1).
2. The full-automatic immunoblotting instrument of claim 1, wherein: the rack (1) is provided with a first driving mechanism (3) for driving the large disc (2.1) to rotate and a second driving mechanism (10) for driving the small disc (9) to rotate.
3. The full-automatic immunoblotting instrument according to claim 1 or 2, wherein: reaction household utensils (6) surface is seted up by deep bid (2.1) edge to deep bid (2.1) center reaction tank (6.3) that extends, reaction household utensils (6) are close to the one end of deep bid (2.1) edge and are rotated with deep bid (2.1) and be connected, and the other end is connected with swing span part (5), be provided with lift actuating mechanism (4) on frame (1), lift actuating mechanism (4) drive swing span part (5) go up and down in the axial direction of deep bid (2.1) to the one end swing of being connected with swing span part (5) on the drive reaction household utensils (6).
4. The full-automatic immunoblotting instrument of claim 3, wherein: limiting groove (7.3) that run through to deep bid (2.1) bottom surface are seted up to deep bid (2.1) edge surface, install rotor plate (7.2) that run through limiting groove (7.3) on deep bid (2.1), the one end of rotor plate (7.2) be provided with the first rotation piece of being connected is rotated to deep bid (2.1) bottom, the other end is provided with rotates the second rotation piece of being connected with reaction household utensils (6) one end rotation, rotor plate (7.2) rotate to the center of deep bid (2.1) or to the edge of deep bid (2.1) round the axis of first rotation piece in limiting groove (7.3).
5. The full-automatic immunoblotting instrument of claim 4, wherein: the surface of the large disc (2.1) is fixedly provided with an elastic piece for elastically tensioning the rotating plate (7.2) to the central area of the large disc (2.1).
6. The full-automatic immunoblotting instrument of claim 4, wherein: the border of deep bid (2.1) can be dismantled and be provided with a plurality of sample mounting bracket (8), sample mounting bracket (8) with spacing groove (7.3) one-to-one, sample box (8.2) can be dismantled and place on sample mounting bracket (8).
7. The full-automatic immunoblotting instrument of claim 1, wherein: the kit comprises a cleaning solution kit (11.1), a substrate solution kit (11.2), a stop solution kit (11.3), a diluent kit (11.4), a distilled water kit (11.5) and a CON binding solution kit (11.6).
8. The full-automatic immunoblotting instrument of claim 1, wherein: each reagent kit is provided with a first injector (12.1) with a needle extending into the reagent kit, and the reagent filling device (13) comprises a first clamping component (13.1) for clamping or separating the first injector (12.1) and a first filling lifting component (13.2) for driving the first clamping component (13.1) to lift.
9. The full-automatic immunoblotting instrument of claim 8, wherein: the reagent filling device (13) further comprises a first pressurizing mechanism (13.3) for pressurizing and injecting the first injector (12.1) in a clamped state.
10. A full-automatic immunoblotting detection method, utilize any one of the above-mentioned 1-6 full-automatic immunoblotting appearance to detect, characterized by that: the detection steps are as follows,
step 1: before detection, a plurality of kits are arranged on a small plate (9), the kits are divided into a cleaning solution kit (11.1), a substrate solution kit (11.2), a stop solution kit (11.3), a diluent kit (11.4), a distilled water kit (11.5) and a CON binding solution kit (11.6), and a reaction vessel (6) and a sample box (8.2) are arranged on a large plate (2.1);
step 2: the large disc (2.1) rotates, the reaction vessel (6) is rotated to a reagent filling station below the reagent filling device (13), the reagent filling device (13) works, cleaning liquid in the cleaning liquid kit (11.1) is filled into a reaction tank (6.3) of the reaction vessel (6), the filling amount is 1ml, the reagent filling device (13) resets, the large disc (2.1) rotates, the next reaction vessel (6) moves to the reagent filling station, the reagent filling device (13) works, and cleaning liquid is filled into the reaction vessel (6) at the reagent filling station;
And step 3: after cleaning liquid is added into all the reaction vessels (6), the reaction vessels (6) are shaken for 1min, and waste liquid in the reaction vessels (6) is sucked and extracted after the cleaning is finished;
and 4, step 4: the large plate (2.1) rotates, the reaction vessel (6) is rotated to a sample filling station below the sample filling mechanism (16), the sample filling mechanism (16) works to suck 10ul of serum samples in the sample box (8.2), the reaction vessel moves to the position, corresponding to the membrane strip, in the reaction vessel (6), the serum samples are filled to the position of the membrane strip, after the completion, the large plate (2.1) rotates, the reaction vessel (6) moves to a reagent filling station, the reagent filling device (13) works to fill 1ml of diluent into the reaction vessel (6) at the reagent filling station, and the actions of adding the diluent and the serum samples are repeated until all the reaction vessels (6) are filled with the serum samples and the diluent;
and 5: shaking the reaction vessel (6) for 30min, diluting the serum sample in the reaction vessel (6) with the diluent, and sucking and extracting waste liquid in the reaction vessel (6) after the dilution is finished;
step 6: the step 2 of filling cleaning solution into the reaction vessel (6) for cleaning is repeated, the reaction vessel (6) is shaken for 5min, after the cleaning is finished, waste liquid in the reaction vessel (6) is sucked and extracted, and the cleaning action in the step is repeated for three times;
And 7: the large disc (2.1) rotates to a reagent filling station, the reagent filling device (13) fills con reagent liquid into a reaction tank (6.3) of the reaction vessel (6), the reaction vessel is shaken for 30min, and waste liquid in the reaction vessel (6) is sucked and extracted after the completion;
and 8: repeating the process of step 6;
and step 9: the large disc (2.1) rotates, the reaction vessel (6) moves to a reagent filling station to be added with a substrate liquid reagent, the reaction vessel (6) is shaken for 10min, and after the reaction vessel (6) is finished, waste liquid in the reaction vessel (6) is sucked and extracted;
step 10: the large disc (2.1) rotates, the reaction vessel (6) moves to a reagent filling station, distilled water is added, the reaction vessel (6) shakes for 1min, and after the reaction vessel (6) is finished, waste liquid in the reaction vessel (6) is sucked and extracted;
step 11: the large disc (2.1) rotates, the reaction vessel (6) moves to the reagent filling station to be added with the stop solution, the reaction vessel (6) shakes for 1min, and after the reaction vessel (6) is finished, waste liquid in the reaction vessel (6) is sucked and extracted.
Technical Field
The invention relates to the technical field of full-automatic immunoblot detection, in particular to a full-automatic immunoblot analyzer and a detection method.
Background
The immunoblotting method is widely used for autoantibody detection, allergen detection and protein detection, and mainly carries out qualitative and semi-quantitative analysis on a detected object.
In order to solve the above problems, in a chinese patent document with an issued publication number of CN207992247U, the full-automatic immunoblotting instrument comprises an instrument body, wherein a moving track is arranged on the instrument body, a membrane strip groove and a sample rack, the membrane strip groove is used for accommodating a blotting membrane, a plurality of sample test tube grooves are formed in the sample rack, the sample test tube grooves are used for placing sample test tubes, a second moving arm is arranged on the moving track, a plurality of waste suction channels and a plurality of reagent adding channels are arranged on the second moving arm, a sample adding needle head is arranged on the first moving arm and is used for extracting sample liquid in the sample test tubes and moving along a first direction to drip the sample liquid onto the blotting membrane, an operator edits a program of an experimental project on a control device in advance, scans bar codes on the sample test tubes before an experiment begins, sequentially places the sample test tubes on the sample rack and places the blotting strips in the membrane strip groove; after starting the instrument, automatically completing the whole experimental process; and finally, shooting by using the CCD, and generating and printing a result report by using a computer to assist in interpreting an experiment result.
The above prior art solutions have the following drawbacks: the film strip grooves are arranged along the length direction of the machine body, so that a large space is occupied, and a long moving track is arranged, so that the whole equipment is large in size and large in occupied space.
Disclosure of Invention
Aiming at the defects in the prior art, the first purpose of the invention is to provide a full-automatic immunity tracing instrument, which reasonably and efficiently utilizes the space structures of a large disk and a small disk, optimizes the space distribution of a reaction tank and a kit, reduces the volume of the whole full-automatic immunity tracing instrument and reduces the occupied space.
The first object of the invention is achieved by the following technical solutions:
a full-automatic immunoblotting instrument comprises a frame, a kit, a reagent filling device, a reaction vessel provided with a membrane strip, a sample box, and a sample filling mechanism for adding sample liquid in the sample box into the reaction vessel are arranged on the frame, the rack is rotatably provided with a large disc and a small disc which are coaxial, the setting height of the small disc is higher than that of the large disc, the reagent kit is provided with a plurality of reagent containers which are arranged on the small disc along the circumferential direction of the small disc, the reagent filling device is arranged at the outer edge of the small disc, the reaction vessels and the sample boxes are provided with a plurality of reagent containers, and are all arranged along the circumference of the large disc, the sample box is arranged at the edge of the large disc, the reaction vessel is arranged between the center of the large disc and the sample box, one side of the rack, which is positioned on the small disc, is provided with a short-stroke track, and the sample filling module is arranged on the short-stroke track and moves along the radial direction of the large disc.
Through adopting above-mentioned technical scheme, the circumference array distribution of reaction tank edge deep bid, the circumference array distribution of reagent box edge deep bid, adopt the deep bid, the deep bid cooperation, it is reasonable, the space structure of deep bid and deep bid is utilized to the high efficiency, the space distribution of reaction tank and reagent box has been optimized, make the volume of whole full-automatic immune mark appearance reduce, reduce occupation space, sample filling mechanism and reagent filling device distribute in the deep bid, the edge position of deep bid, further space occupation volume of each part of full-automatic immune mark appearance has been optimized, whole equipment tends to the miniaturization, high efficiency and automation.
The invention is further configured to: the rack is provided with a first driving mechanism for driving the large disc to rotate and a second driving mechanism for driving the small disc to rotate.
Through adopting above-mentioned technical scheme, first actuating mechanism and second actuating mechanism realize the automated control to big dish, minidisc, realize the automatic rotation of big dish, minidisc, promote whole immunity detector's automation level.
The invention is further configured to: the reaction vessel surface is provided with a reaction tank extending from the edge of the large plate to the center of the large plate, one end of the reaction vessel close to the edge of the large plate is rotatably connected with the large plate, the other end of the reaction vessel is connected with a swing frame component, the rack is provided with a lifting driving mechanism, and the lifting driving mechanism drives the swing frame component to lift in the axial direction of the large plate so as to drive one end of the reaction vessel connected with the swing frame component to swing.
Through adopting above-mentioned technical scheme, the swing span part goes up and down under lift actuating mechanism's drive to the one end luffing motion of drive reaction household utensils, the other end and deep bid edge rotate to be connected, at the wobbling in-process, the liquid in the reaction tank reciprocates along the length direction of reaction tank on the reaction household utensils, thereby the abundant contact that can be abundant with reagent or sample liquid of membrane strip in the messenger reaction tank, the detected data that obtains is more accurate, science, and detection effect is better.
The invention is further configured to: the surface of the edge of the large plate is provided with a limiting groove which penetrates through the bottom surface of the large plate, a rotating plate which penetrates through the limiting groove is installed on the large plate, one end of the rotating plate is provided with a first rotating piece which is rotatably connected with the bottom of the large plate, the other end of the rotating plate is provided with a second rotating piece which is rotatably connected with one end of a reaction vessel, and the rotating plate rotates towards the center of the large plate or towards the edge of the large plate around the axis of the first rotating piece in the limiting groove.
Through adopting above-mentioned technical scheme, the one end that reaction household utensils and rotor plate are connected can be close to the deep bid or keep away from the deep bid center at the wobbling in-process, and the rotor plate rotates with the deep bid to be connected the setting, can make the whole deep bid center of being close to of reaction household utensils or keep away from, ensures the wobbling normal clear of reaction household utensils.
The invention is further configured to: and an elastic piece for elastically tensioning the rotating plate to the central area of the large disc is fixedly arranged on the surface of the large disc.
Through adopting above-mentioned technical scheme, the elastic component is taut with the rotor plate to the regional elasticity in big dish center, makes to receive the effect of elastic tension under the reaction household utensils is in quiescent condition, and the quiescent condition that keeps stable is motionless, and during the swing, the elastic force also makes the rotor plate carry out normal rotation not influenced.
The invention is further configured to: the border of deep bid can be dismantled and be provided with a plurality of sample mounting bracket, the sample mounting bracket with the spacing groove one-to-one, the sample box can be dismantled and place on the sample mounting bracket.
Through adopting above-mentioned technical scheme, the sample mounting bracket is located the deep bid edge, and corresponding with placing the household utensils for the position of sample mounting bracket is very close with placing the household utensils distance, and sample filling mechanism only need move short stroke, alright realize adding the serum sample liquid in the sample box in the reaction household utensils, makes filling efficiency higher, and whole instrument operation is got up more high-efficient.
The invention is further configured to: the kit comprises a cleaning solution kit, a substrate solution kit, a stop solution kit, a diluent kit, a distilled water kit and a CON binding solution kit.
By adopting the technical scheme, the kit is divided into a plurality of types, the cleaning solution in the cleaning solution kit is mainly used for cleaning the reaction tank, the substrate solution in the substrate solution kit is mainly used for reacting with the membrane strip for color development, the distilled water in the distilled water kit is also colored, the reading is inaccurate, the substrate solution is cleaned by the distilled water so as to be convenient for carrying out next test detection quickly, the stop solution in the stop solution kit is used for stopping the reaction after the substrate solution reacts on the membrane strip, and the binding solution in the CON binding solution kit refers to the binding of the anti-human IgG monoclonal antibody and the antibody marked by horseradish peroxidase.
The invention is further configured to: each reagent kit is provided with a first injector with a needle extending into the reagent kit, and the reagent filling device comprises a first clamping part for clamping or separating the first injector and a first filling lifting part for driving the first clamping part to lift.
By adopting the technical scheme, the reagent filling device can not only clamp the first injector, but also drive the clamped first injector to move up and down, the lifted first injector can be closer to the reaction tank on the reaction vessel, so that the liquid in the first injector can be accurately injected into the reaction tank of the reaction vessel, and the detection accuracy is improved.
The invention is further configured to: the reagent filling device further comprises a first pressurizing mechanism for pressurizing and injecting the first injector in the clamped state.
Through adopting above-mentioned technical scheme, first loading system is to first syringe pressurization injection, makes in the liquid high efficiency injection reaction tank in the first syringe, and first loading system's setting for automatic injection serum sample and reagent are accomplished to first syringe, reach better automatic level.
The second purpose of the invention is to provide a full-automatic immunoblotting detection method, which is efficient and automatic, the whole detection process is realized by a full-automatic immunoblotting instrument, and more accurate and scientific detection results can be obtained.
The second objective of the invention is achieved by the following technical solutions:
a full-automatic immunoblotting detection method utilizes any one of the above-mentioned full-automatic immunoblotting instruments to make detection, and its detection step is as follows,
step 1: before detection, a plurality of kits are arranged on a small disc, the kits are divided into a cleaning solution kit, a substrate solution kit, a stop solution kit, a diluent kit, a distilled water kit and a CON binding solution kit, and a reaction vessel and a sample box are arranged on a large disc;
Step 2: rotating the large disc, rotating the reaction vessel to a reagent filling station below the reagent filling device, operating the reagent filling device, filling the cleaning solution in the cleaning solution reagent box into a reaction tank of the reaction vessel, wherein the filling amount is 1ml, resetting the reagent filling device, rotating the large disc, moving the next reaction vessel to the reagent filling station, operating the reagent filling device, and filling the cleaning solution into the reaction vessel at the reagent filling station; and step 3: after cleaning liquid is added into all reaction vessels, shaking the reaction vessels for 1min, and sucking and extracting waste liquid in the reaction vessels after cleaning is finished;
and 4, step 4: rotating the large plate, rotating the reaction vessel to a sample filling station below the sample filling mechanism, operating the sample filling mechanism, sucking 10ul of serum samples in the sample box, moving the serum samples to a position corresponding to the membrane strip in the reaction vessel, filling the serum samples to the position of the membrane strip, after the completion, rotating the large plate, moving the reaction vessel to a reagent filling station, operating the reagent filling device, filling 1ml of diluent into the reaction vessel at the reagent filling station, and repeatedly adding the diluent and the serum samples until all the reaction vessels are filled with the serum samples and the diluent;
And 5: shaking the reaction vessel for 30min, diluting the serum sample in the reaction vessel with the diluent, and sucking and extracting waste liquid in the reaction vessel after dilution is completed;
step 6: the
and 7: rotating the large disc to a reagent filling station, filling con reagent liquid into a reaction tank of a reaction vessel by a reagent filling device, shaking for 30min, and sucking and extracting waste liquid in the reaction vessel after completion;
and 8: repeating the process of
and step 9: rotating the large disc, moving the reaction vessel to a reagent filling station, adding a substrate solution reagent, shaking the reaction vessel for 10min, and sucking and extracting waste liquid in the reaction vessel after the reaction vessel is finished;
step 10: rotating the large disc, moving the reaction vessel to a reagent filling station, adding distilled water, shaking the reaction vessel for 1min, and sucking and extracting waste liquid in the reaction vessel after the reaction vessel is finished;
step 11: and (3) rotating the large disc, moving the reaction vessel to a reagent filling station, adding the stop solution, shaking the reaction vessel for 1min, and sucking and extracting the waste liquid in the reaction vessel after the completion.
Through adopting above-mentioned technical scheme, reagent and serum sample add the reaction tank through reagent filling mechanism and sample filling mechanism respectively in, wave the reaction tank through automatic for membrane strip and reagent, the serum sample contact in the reaction tank is more abundant, and multiple kit uses according to different detection needs, and the condition to antibody detection is more sufficient, and the data that obtain are more complete.
In conclusion, the beneficial technical effects of the invention are as follows:
1. the space structure of the large disc and the small disc is reasonably and efficiently utilized, the space distribution of the reaction tank and the reagent kit is optimized, the volume of the whole full-automatic immunoblotting instrument is reduced, and the occupied space is reduced;
2. in the process of swinging the reaction vessel, the liquid in the reaction tank flows back and forth along the length direction of the reaction tank, so that the membrane strip in the reaction tank can be fully contacted with the reagent or sample liquid, the obtained detection data is more accurate and scientific, and the detection effect is better;
3. the detection method in the invention is efficient and automatic, the whole detection process is realized by a full-automatic immunoblotting instrument, and more accurate and scientific detection results can be obtained;
4. the invention adopts the clamping of the disposable syringe as a method for filling the reagent, is different from the pipeline structure adopted by the prior full-automatic immunoblotting instrument, and avoids cross contamination.
Drawings
FIG. 1 is a schematic diagram of the overall structure of example 1, which is used for embodying the overall structure of the fully automatic immunoblotting apparatus;
FIG. 2 is an enlarged schematic view of section A of FIG. 1, illustrating the construction and mounting location of the swing frame assembly and reaction vessel;
FIG. 3 is a partial schematic structural view of the
FIG. 4 is a schematic view showing a partial explosion of
FIG. 5 is a second schematic view of the partial explosion of
FIG. 6 is a schematic view showing a part of the explosion of the third embodiment of the present invention, which is used to show the structural components of the lifting and lowering of the head assembly and the rotation of the large plate;
FIG. 7 is an enlarged view of the portion B of FIG. 6 for showing the installation and shape configuration of the reaction vessel and the installation chassis;
FIG. 8 is a second partial schematic structural view of
FIG. 9 is an enlarged view of the portion C of FIG. 8, showing the construction of a second pulley assembly for driving the rotation of the small disk;
FIG. 10 is a schematic view showing the structure of a second side plate, a second carrier, a tray and a reagent cartridge in example 1, for embodying the reagent cartridge containing different reagent liquids;
FIG. 11 is a fourth schematic view of a partial explosion of example 1 for embodying the installation position of the waste liquid suction mechanism;
FIG. 12 is an enlarged view of the portion D in FIG. 11, showing the configuration of the waste liquid suction mechanism;
fig. 13 is a second schematic view of the whole structure of the
fig. 14 is a schematic structural view of the second side plate, the inclined support plate and the reagent filling device in
fig. 15 is an exploded schematic view of the second side plate and the reagent filling device in
FIG. 16 is an enlarged view of section E of FIG. 15 illustrating the mounting engagement of the worm gear;
FIG. 17 is a schematic structural view of a second holding member for embodying a second jaw-shaped configuration for holding an injection rod of the first syringe in accordance with
fig. 18 is a schematic partial structural view three of the
FIG. 19 is a schematic view showing a partial explosion of the bottom of the nozzle placement frame in accordance with example 1, showing the position and configuration of the respective parts;
fig. 20 is an enlarged schematic view of part F of fig. 19 for illustrating a connection relationship between the sixth driving mechanism and the nozzle placement frame;
fig. 21 is a schematic diagram six of the partial explosion of the
FIG. 22 is an enlarged view of section G of FIG. 21, illustrating the mounting engagement between the sample filling mechanism and the lateral mount;
FIG. 23 is a fourth partial schematic structural view showing the constitution and mounting position of a seventh driving mechanism in accordance with
fig. 24 is a schematic structural view of the sample filling mechanism in example 1, which is used to embody the constitution of each component of the sample filling mechanism;
fig. 25 is an exploded view of the second syringe and the mouthpiece in example 1 for embodying the respective component constitutions of the second syringe.
In the figure, 1, a frame; 1.1, a first side plate; 1.2, a bottom plate; 1.3, a first bearing platform; 1.4, a second bearing platform; 1.5, a second through hole; 1.6, a second mounting shaft sleeve; 1.7, a second bearing; 1.8, a rotating shaft; 1.10, a second side plate; 1.11, an inclined support plate; 2.1, a large disc; 2.2, a first through hole; 2.3, a first mounting shaft sleeve; 2.4, a first bearing; 3. a first drive mechanism; 3.1, a first driving motor; 3.2, a first pulley assembly; 3.3, a driving shaft; 3.4, a first base sleeve disc; 3.5, a first driving wheel; 3.6, a first driven wheel; 3.7, a first belt; 4. a lifting drive mechanism; 4.1, a lifting driving motor; 4.2, a transmission component; 4.3, a telescopic shaft; 4.4, a chassis; 4.5, a telescopic sleeve; 4.6, a transmission disc; 4.7, a transmission joint; 4.8, a first connecting shaft; 4.9, a second connecting shaft; 4.10, a first connection hole; 4.11, a second connecting hole; 4.12, a third connecting shaft; 4.13, lifting holes; 4.14, telescopic holes; 4.15, a limiting sleeve; 5. a swing frame member; 5.1, a lifting disc; 5.2, swinging the mounting rack; 5.3, a first guide rod; 5.4, a first guide hole; 5.5, a frame body; 5.6, swinging the rotating shaft; 5.7, supporting rods; 5.8, supporting the roller; 5.9, a first elastic limiting sheet; 6. a reaction vessel; 6.1, a first hook; 6.2, a first card slot; 6.3, a reaction tank; 6.4, a second hook; 6.5, a second card slot; 7.1, installing a bottom frame; 7.2, rotating the plate; 7.3, a limiting groove; 7.4, extending grooves; 7.5, extending the side plate; 7.6, a first articulated shaft; 7.7, a second articulated shaft; 7.8, a first spring; 7.9, a first connecting plate; 7.10, a partition plate; 8. a sample mounting rack; 8.1, installing a cavity; 8.2, a sample box; 8.3, placing holes; 8.4, a serum sample test tube; 8.5, an elastic clamping piece; 8.6, clamping a groove; 9. a small disc; 9.1, through holes; 9.2, a notch; 10. a second drive mechanism; 10.1, a second driving motor; 10.2, a second pulley assembly; 10.3, a second driving wheel; 10.4, a second driven wheel; 10.5, a second belt; 11.1, a cleaning solution kit; 11.2, a substrate solution kit; 11.3, stop solution kit; 11.4, diluent kit; 11.5, a distilled water kit; 11.6, CON binding solution kit; 12.1, a first syringe; 12.2, liquid outlet holes; 12.3, a first syringe; 12.4, fixing the sleeve; 13. a reagent filling device; 13.1, a first clamping component; 13.2, a first filling lifting component; 13.3, a first pressurizing mechanism; 13.4, filling a lifting motor; 13.5, a first ball screw pair; 13.6, a first subplate; 13.7, a third driving motor; 13.8, a third pulley assembly; 13.9, a first clamping seat; 13.10, a third driving wheel; 13.11, a third driven wheel; 13.12, a third belt; 13.13, a tension wheel A; 13.14, a through hole A; 13.15, mounting a bearing A; 13.16, a first threaded bushing; 13.17, a second guide column; 13.18, a third guide column; 13.19, a second guide hole; 13.20, a third guide hole; 13.21, a first clamping cushion block; 13.22, a first clamping groove; 13.23, elastic gaskets; 13.24, a fifth driving motor; 13.25, turbine; 13.26, a worm; 13.27, a first bushing; 13.29, a first drive rod; 13.30, a first mounting block; 13.31, caulking grooves; 13.32, a fourth bearing; 13.33, a second mounting block; 13.34, a fourth guide rod; 13.35, a guide slide block; 13.36, a fourth guide hole; 13.37, a second clamping seat; 13.38, ball nut; 13.39, a first slider; 13.40, a second clamping member; 13.41, a second jaw; 13.42, a second bidirectional screw rod; 13.43, a fourth pulley assembly; 13.44, a fourth driving motor; 13.45, a fourth driving wheel; 13.46, a fourth driven wheel; 13.47, a fourth belt; 13.48, a tensioning wheel B; 13.49, through hole B; 13.50, mounting a bearing B; 13.51, a second threaded bushing; 13.52, a fourth guide post; 13.53, fourth guide hole; 13.54, a second clamping cushion block; 13.55, a second clamping groove; 13.56, mounting plate; 13.57, fixing blocks; 13.58, a compression bar; 13.59, a first jaw; 13.60, a first bidirectional screw rod; 14. a carriage; 14.1, a third side plate; 14.2, a transverse mounting seat; 14.3, a channel; 14.4, a track plate; 14.5, a first slide rail; 14.6, placing a suction nozzle rack; 14.7, a first track slider; 14.8, a first sliding groove; 14.9, a first elongated groove; 14.10, a first extension block; 14.11, a first limiting block; 14.12, a first bolt; 15. a sixth drive mechanism; 15.1, a sixth driving motor; 15.2, a sixth pulley assembly; 15.3, a sixth driving wheel; 15.4, a sixth driven wheel; 15.5, a sixth belt; 15.6, suction nozzle placing holes; 15.7, a suction nozzle; 15.8, short stroke track; 15.9, a second slide rail; 16. a sample filling mechanism; 16.1, connecting a backing plate; 16.2, a second track slider; 16.3, a second sliding groove; 16.4, a second elongated groove; 16.5, a second extension block; 16.6, a second bolt; 16.7, a second limiting block; 16.8, a seventh drive mechanism; 16.9, a seventh driving motor; 16.10, a seventh pulley assembly; 16.11, a seventh driving wheel; 16.12, a seventh driven wheel; 16.13, a seventh belt; 16.14, a work box; 16.15, a second syringe; 16.16, an injection control assembly; 16.17, a lifting driving component; 16.18, moving the driving motor; 16.19, a second ball screw pair; 16.20, a linkage plate; 16.21, a fifth guide rod; 16.22, embedding holes; 16.23, a guide shaft sleeve; 16.24, a fifth guide hole; 16.25, controlling the motor; 16.26, a control screw rod; 16.28, round holes; 16.29, telescopic guide rods; 16.30, ball nut; 16.31, a driving block; 16.32, a sixth guide hole; 16.33, syringe barrel; 16.34, a piston; 16.35, an injection rod; 16.36, moving the hole; 16.37, connecting hole; 17. a waste liquid suction mechanism; 17.1, a suction driving motor; 17.2, a straw; 17.4, connecting rod assemblies; 17.5, a window; 17.6, a first connecting rod; 17.7, a second connecting rod; 17.8, connecting rods; 17.9, a third hinge shaft; 17.10, a fourth articulated shaft; 17.11, a hinged seat; 17.12, connecting lugs; 17.13, a fifth articulated shaft; 17.14, fixing the chuck.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
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